BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 168.24 1.28 2 3 204 201 168.24 1.28 3 3 205 202 166.31 0.60 4 3 224 221 112.39 0.67 5 3 227 224 112.22 0.63 6 4 35 31 669.82 1.26 7 4 36 32 669.76 1.26 8 4 37 33 669.75 1.26 9 4 38 34 669.74 1.27 10 4 205 201 168.23 1.28 11 5 37 32 669.76 1.26 12 5 38 33 669.75 1.27 13 5 221 216 112.36 0.67 14 5 227 222 112.37 0.67 15 5 247 242 19.77 0.31 16 6 38 32 669.76 1.26 17 6 40 34 669.75 1.27 18 6 90 84 351.21 0.32 19 6 124 118 302.88 4.05 20 6 161 155 297.49 6.56 21 6 205 199 169.75 1.04 22 6 207 201 167.87 1.36 23 6 227 221 112.45 0.69 24 6 228 222 112.25 0.62 25 6 229 223 112.25 0.62 26 7 37 30 669.84 1.26 27 7 38 31 669.81 1.26 28 7 39 32 669.78 1.26 29 7 40 33 669.78 1.26 30 7 229 222 112.36 0.67 31 7 240 233 50.66 0.51 32 7 243 236 43.75 0.66 33 7 244 237 35.97 0.07 34 7 247 240 21.16 0.40 35 8 40 32 669.78 1.26 36 8 42 34 669.77 1.26 37 8 229 221 112.64 0.62 38 8 230 222 112.24 0.62 39 8 231 223 112.23 0.62 40 9 40 31 669.81 1.26 41 9 41 32 669.79 1.26 42 9 42 33 669.77 1.26 43 9 231 222 112.34 0.67 44 9 235 226 83.92 1.01 45 9 240 231 50.66 0.51 46 10 42 32 669.79 1.26 47 10 64 54 456.17 0.94 48 10 92 82 356.12 0.09 49 10 95 85 355.50 0.32 50 10 230 220 113.97 0.15 51 10 231 221 112.59 0.63 52 10 232 222 112.28 0.65 53 10 235 225 86.27 0.02 54 11 14 3 886.73 4.26 55 11 20 9 670.82 1.28 56 11 23 12 670.75 1.25 57 11 24 13 670.78 1.28 58 11 27 16 670.74 1.26 59 11 41 30 669.84 1.26 60 11 42 31 669.82 1.26 61 11 43 32 669.79 1.26 62 11 51 40 556.35 1.42 63 11 235 224 107.62 2.22 64 11 236 225 86.20 0.04 65 12 44 32 669.81 1.26 66 12 64 52 456.30 0.99 67 12 95 83 356.28 0.04 68 12 97 85 356.42 0.10 69 12 232 220 113.97 0.15 70 13 44 31 669.82 1.26 71 14 44 30 669.82 1.26 72 14 236 222 112.22 0.62 73 15 19 4 837.09 0.45 74 15 20 5 835.30 0.44 75 15 47 32 669.45 1.17 76 16 19 3 850.38 0.58 77 16 20 4 839.88 0.66 78 17 20 3 870.61 3.89 79 17 21 4 843.47 1.13 80 17 50 33 626.93 3.04 81 18 21 3 851.77 5.28 82 18 22 4 843.47 1.13 83 19 22 3 859.39 6.83 84 19 23 4 841.73 0.27 85 20 23 3 851.98 4.68 86 20 24 4 840.16 1.04 87 20 47 27 668.31 0.65 88 20 50 30 577.47 3.50 89 20 51 31 565.58 6.04 90 20 54 34 544.06 1.38 91 21 24 3 879.80 3.74 92 21 25 4 841.66 1.79 93 21 54 33 542.14 2.23 94 22 25 3 857.61 7.35 95 22 26 4 843.82 0.73 96 23 26 3 857.63 3.79 97 23 27 4 848.84 0.57 98 23 235 212 111.31 0.87 99 23 236 213 90.27 1.52 100 23 237 214 86.25 0.03 101 23 238 215 83.05 1.40 102 24 27 3 856.15 3.08 103 24 28 4 851.67 0.85 104 24 54 30 502.79 21.80 105 24 55 31 502.79 21.76 106 25 28 3 865.58 1.62 107 25 29 4 856.15 3.09 108 26 29 3 871.81 4.74 109 26 238 212 77.22 0.42 110 26 241 215 58.36 0.12 111 27 30 3 883.76 4.29 112 27 55 28 502.13 22.09 113 27 59 32 458.44 0.54 114 27 235 208 102.48 0.10 115 27 237 210 86.15 0.06 116 27 240 213 57.41 0.09 117 27 241 214 50.55 0.46 118 28 54 26 502.78 21.79 119 28 55 27 502.12 22.08 120 28 56 28 502.03 22.09 121 28 58 30 501.63 22.11 122 28 59 31 458.56 0.59 123 30 58 28 458.42 0.55 124 30 241 211 50.52 0.44 125 30 244 214 47.79 0.32 126 30 245 215 47.78 0.33 127 30 248 218 44.54 0.27 128 32 39 7 670.84 1.15 129 32 58 26 456.78 0.82 130 32 59 27 456.78 0.82 131 32 248 216 30.19 0.58 132 33 36 3 873.35 12.54 133 33 37 4 836.38 1.25 134 34 37 3 849.25 2.66 135 34 58 24 456.64 0.88 136 37 244 207 40.97 2.02 137 37 247 210 22.46 0.91 138 37 248 211 16.91 0.20 139 38 205 167 169.81 1.01 140 39 59 20 456.64 0.88 141 39 60 21 456.62 0.88 142 39 244 205 35.76 0.17 143 40 60 20 456.62 0.88 144 40 61 21 456.62 0.88 145 40 62 22 456.61 0.88 146 40 90 50 356.57 0.10 147 41 51 10 501.57 22.02 148 41 55 14 457.74 1.09 149 41 60 19 456.68 0.89 150 41 61 20 456.63 0.89 151 41 62 21 456.62 0.88 152 42 62 20 456.62 0.89 153 42 63 21 456.57 0.87 154 42 64 22 456.25 0.98 155 42 90 48 356.82 0.04 156 42 91 49 356.69 0.09 157 42 92 50 356.38 0.01 158 42 124 82 356.23 0.03 159 42 230 188 113.97 0.15 160 43 47 4 823.75 9.62 161 43 48 5 556.74 1.62 162 43 51 8 545.57 3.96 163 43 62 19 456.66 0.89 164 43 64 21 456.58 0.86 165 44 47 3 847.65 3.52 166 44 48 4 836.25 3.38 167 44 64 20 456.60 0.87 168 45 48 3 851.16 3.71 169 47 50 3 854.83 4.05 170 47 51 4 854.20 4.39 171 48 51 3 854.21 4.40 172 48 52 4 850.62 6.19 173 48 61 13 457.19 0.86 174 48 62 14 457.09 0.89 175 48 63 15 456.85 0.82 176 48 88 40 357.13 0.04 177 49 52 3 856.16 3.42 178 49 53 4 849.95 6.52 179 50 53 3 868.32 2.64 180 50 54 4 856.18 3.42 181 51 54 3 862.79 0.12 182 51 55 4 844.90 2.21 183 51 61 10 457.28 0.90 184 52 55 3 862.78 0.12 185 52 56 4 856.47 3.55 186 52 57 5 838.20 0.63 187 52 59 7 674.83 0.35 188 52 60 8 458.51 0.65 189 52 61 9 457.27 0.90 190 55 59 4 842.37 1.41 191 55 61 6 606.38 31.02 192 56 59 3 887.97 0.29 193 59 244 185 42.16 2.31 194 60 89 29 356.80 0.04 195 60 90 30 356.75 0.06 196 61 89 28 356.82 0.04 197 61 90 29 356.77 0.05 198 62 88 26 356.92 0.04 199 62 90 28 356.77 0.05 200 63 78 15 358.25 0.03 201 63 83 20 357.51 0.06 202 63 88 25 357.25 0.01 203 63 90 27 356.85 0.04 204 63 91 28 356.80 0.04 205 63 92 29 356.81 0.04 206 64 92 28 356.81 0.04 207 65 92 27 356.90 0.06 208 65 98 33 357.09 0.10 209 65 112 47 357.50 0.04 210 66 78 12 358.40 0.03 211 66 79 13 358.27 0.01 212 66 80 14 358.25 0.01 213 66 91 25 357.22 0.01 214 66 93 27 357.17 0.03 215 66 112 46 357.54 0.04 216 66 116 50 357.60 0.01 217 67 77 10 358.69 0.08 218 67 78 11 358.54 0.01 219 67 79 12 358.28 0.01 220 67 112 45 357.62 0.08 221 68 108 40 357.60 0.07 222 68 111 43 357.60 0.07 223 68 112 44 357.62 0.08 224 69 74 5 701.54 65.00 225 69 79 10 358.80 0.14 226 70 74 4 892.26 2.50 227 70 79 9 358.81 0.15 228 70 81 11 358.81 0.13 229 71 79 8 358.84 0.14 230 71 81 10 358.84 0.14 231 71 82 11 358.92 0.18 232 74 77 3 864.82 0.56 233 78 82 4 846.50 3.91 234 79 82 3 862.82 4.25 235 79 83 4 846.50 3.90 236 80 83 3 862.44 0.10 237 80 84 4 846.50 3.90 238 80 115 35 363.58 0.36 239 80 116 36 362.90 0.02 240 80 119 39 367.06 1.20 241 80 121 41 367.01 1.18 242 81 84 3 846.85 3.73 243 81 85 4 846.49 3.90 244 81 119 38 462.30 0.41 245 82 85 3 859.24 1.54 246 82 86 4 835.19 1.60 247 83 86 3 852.50 4.91 248 83 87 4 832.20 3.10 249 83 88 5 685.50 69.10 250 83 121 38 471.74 2.37 251 84 87 3 840.66 1.01 252 84 119 35 483.00 2.96 253 84 120 36 489.31 0.18 254 84 121 37 480.94 2.10 255 88 121 33 526.40 1.57 256 89 122 33 526.33 1.53 257 90 122 32 526.41 1.57 258 90 161 71 526.36 1.54 259 91 116 25 526.66 1.59 260 91 121 30 526.64 1.58 261 91 122 31 526.58 1.60 262 91 123 32 526.48 1.59 263 91 124 33 526.47 1.59 264 92 95 3 906.94 1.52 265 92 124 32 526.49 1.60 266 92 126 34 526.46 1.59 267 92 230 138 113.95 0.15 268 93 109 16 526.67 1.63 269 93 112 19 526.85 1.55 270 93 113 20 526.82 1.55 271 93 116 23 526.88 1.56 272 93 123 30 526.69 1.61 273 93 124 31 526.54 1.59 274 93 125 32 526.49 1.60 275 93 126 33 526.47 1.60 276 94 99 5 750.12 40.34 277 94 109 15 526.67 1.63 278 94 126 32 526.49 1.60 279 95 98 3 863.36 3.71 280 95 99 4 830.50 3.35 281 95 126 31 526.54 1.59 282 95 165 70 526.51 1.59 283 96 99 3 843.10 2.02 284 96 100 4 838.87 0.09 285 96 165 69 526.52 1.59 286 96 167 71 526.25 1.53 287 96 170 74 493.53 1.30 288 97 100 3 848.50 0.68 289 97 101 4 822.97 7.11 290 98 102 4 639.83 26.45 291 98 103 5 639.82 26.45 292 98 104 6 541.57 8.11 293 99 102 3 871.52 0.07 294 99 104 5 527.63 1.14 295 99 106 7 528.54 1.59 296 99 109 10 527.72 1.18 297 99 126 27 526.76 1.60 298 99 127 28 526.73 1.59 299 104 108 4 826.68 1.21 300 104 112 8 821.28 3.85 301 105 108 3 851.57 1.08 302 105 109 4 845.02 2.63 303 106 109 3 852.00 0.86 304 106 110 4 852.00 0.86 305 106 145 39 527.12 1.57 306 106 146 40 527.01 1.60 307 106 149 43 527.12 1.57 308 107 110 3 874.63 9.63 309 107 111 4 853.44 0.15 310 108 111 3 853.44 0.14 311 108 112 4 846.93 0.06 312 109 112 3 855.48 1.17 313 109 113 4 848.24 0.59 314 109 125 16 527.06 1.64 315 109 150 41 527.31 1.56 316 110 113 3 856.12 2.87 317 110 114 4 855.62 3.10 318 110 149 39 527.30 1.56 319 110 150 40 527.31 1.56 320 110 152 42 532.35 0.57 321 110 153 43 532.34 0.55 322 111 114 3 856.11 2.87 323 111 115 4 853.49 4.17 324 112 115 3 859.23 1.29 325 112 116 4 853.49 4.17 326 113 116 3 863.32 0.75 327 113 117 4 844.90 2.29 328 113 123 10 528.31 1.41 329 113 125 12 527.29 1.56 330 113 153 40 539.70 3.06 331 113 160 47 528.89 1.52 332 114 117 3 854.03 5.40 333 114 118 4 831.63 4.34 334 114 153 39 533.03 0.28 335 115 118 3 843.24 1.45 336 115 119 4 841.40 0.54 337 116 119 3 856.88 0.55 338 116 120 4 843.24 1.46 339 116 121 5 787.49 4.10 340 116 123 7 539.25 2.83 341 117 120 3 841.39 0.55 342 117 121 4 810.46 3.63 343 117 153 36 703.89 2.10 344 117 154 37 695.62 2.04 345 117 155 38 719.22 2.98 346 117 160 43 695.43 1.98 347 122 159 37 778.53 0.25 348 123 159 36 778.65 0.24 349 123 160 37 778.59 0.23 350 123 161 38 778.61 0.24 351 124 161 37 778.61 0.24 352 124 163 39 778.61 0.24 353 124 230 106 113.94 0.17 354 125 150 25 778.91 0.24 355 125 160 35 778.72 0.25 356 125 161 36 778.67 0.26 357 125 162 37 778.62 0.24 358 125 163 38 778.62 0.24 359 126 163 37 778.62 0.24 360 126 165 39 778.62 0.24 361 126 230 104 114.02 0.14 362 127 143 16 779.22 0.17 363 127 146 19 779.14 0.17 364 127 147 20 779.11 0.19 365 127 150 23 779.11 0.19 366 127 162 35 778.73 0.25 367 127 163 36 778.66 0.26 368 127 164 37 778.63 0.24 369 127 165 38 778.64 0.26 370 127 188 61 229.07 4.00 371 128 143 15 779.24 0.18 372 128 146 18 779.22 0.17 373 128 164 36 778.66 0.26 374 128 165 37 778.64 0.26 375 128 167 39 778.58 0.26 376 129 133 4 828.09 0.27 377 129 134 5 828.05 0.26 378 129 139 10 779.34 0.15 379 129 142 13 779.29 0.18 380 129 143 14 779.28 0.18 381 129 164 35 778.73 0.25 382 129 167 38 778.61 0.26 383 129 168 39 778.58 0.26 384 130 133 3 859.35 1.33 385 130 134 4 856.91 0.12 386 130 167 37 778.62 0.27 387 130 168 38 778.59 0.27 388 131 134 3 856.91 0.12 389 131 135 4 842.12 7.29 390 131 168 37 778.60 0.27 391 131 172 41 742.11 4.11 392 131 174 43 707.85 3.18 393 132 135 3 867.18 3.31 394 132 136 4 830.25 0.80 395 132 138 6 824.80 0.18 396 133 136 3 866.99 3.46 397 133 137 4 837.22 4.29 398 133 138 5 790.72 5.29 399 133 142 9 780.60 0.67 400 134 137 3 849.53 1.86 401 134 139 5 789.53 5.13 402 134 168 34 778.64 0.26 403 134 180 46 236.57 1.95 404 138 141 3 845.01 7.79 405 138 142 4 827.77 0.21 406 139 142 3 851.19 0.78 407 139 143 4 827.77 0.21 408 140 143 3 856.28 2.14 409 140 144 4 827.76 0.21 410 140 187 47 229.27 4.04 411 141 144 3 856.29 2.14 412 141 145 4 830.53 1.59 413 142 145 3 848.98 1.52 414 142 146 4 838.03 4.70 415 143 146 3 857.02 1.79 416 143 147 4 849.85 1.21 417 143 164 21 778.75 0.24 418 143 184 41 229.29 4.04 419 143 188 45 229.12 4.00 420 143 191 48 229.06 4.00 421 144 147 3 871.55 7.27 422 144 148 4 849.85 1.22 423 144 191 47 229.06 4.00 424 145 148 3 871.03 7.53 425 145 149 4 849.88 1.23 426 146 149 3 858.95 0.87 427 146 150 4 861.88 0.59 428 147 150 3 871.33 1.68 429 147 151 4 840.58 5.78 430 147 188 41 229.08 4.00 431 147 191 44 229.05 3.99 432 148 151 3 868.88 2.90 433 148 195 47 228.30 3.85 434 150 153 3 862.13 0.74 435 150 162 12 778.75 0.24 436 151 154 3 866.14 2.75 437 151 192 41 228.63 3.97 438 151 195 44 227.47 4.19 439 151 196 45 227.12 4.07 440 154 157 3 892.35 4.66 441 154 160 6 778.77 0.24 442 154 204 50 203.44 0.96 443 156 159 3 850.89 0.98 444 157 192 35 228.08 3.93 445 157 196 39 226.16 3.58 446 157 200 43 203.55 0.92 447 157 203 46 203.44 0.95 448 157 204 47 203.36 0.92 449 158 203 45 203.44 0.96 450 158 205 47 203.35 0.92 451 160 204 44 203.28 0.94 452 161 205 44 200.81 0.94 453 161 207 46 195.34 0.99 454 162 188 26 228.69 3.93 455 162 192 30 228.15 3.96 456 162 204 42 203.36 0.92 457 162 205 43 202.85 1.12 458 162 206 44 196.20 0.58 459 162 207 45 195.35 1.00 460 163 207 44 195.34 1.00 461 163 208 45 195.32 1.01 462 163 209 46 194.97 0.97 463 163 230 67 113.95 0.16 464 164 184 20 228.88 3.89 465 164 185 21 229.03 3.99 466 164 188 24 228.86 3.90 467 164 207 43 195.62 0.89 468 164 208 44 195.25 1.03 469 164 209 45 195.02 0.97 470 165 170 5 837.40 3.40 471 166 169 3 876.26 1.54 472 166 170 4 841.23 1.49 473 166 176 10 229.60 4.03 474 166 208 42 195.80 1.02 475 166 209 43 195.33 1.01 476 166 210 44 193.63 1.33 477 167 170 3 875.33 4.79 478 167 172 5 825.80 2.41 479 168 172 4 840.28 2.39 480 168 174 6 779.42 0.27 481 168 176 8 255.50 10.70 482 168 180 12 235.11 1.50 483 169 172 3 858.75 6.09 484 169 174 5 616.70 36.87 485 169 176 7 235.00 1.48 486 176 180 4 833.79 5.44 487 176 208 32 200.68 0.64 488 177 180 3 848.02 0.83 489 177 181 4 843.75 0.47 490 178 181 3 843.75 0.47 491 178 182 4 843.77 0.47 492 178 219 41 120.73 1.00 493 178 220 42 120.26 0.77 494 178 223 45 120.28 0.78 495 179 182 3 859.53 0.43 496 179 183 4 845.94 1.53 497 180 183 3 861.49 5.76 498 180 184 4 861.49 5.75 499 181 184 3 861.49 5.75 500 181 185 4 846.44 1.78 501 181 208 27 201.81 0.57 502 181 226 45 117.75 0.14 503 182 185 3 856.12 3.06 504 182 186 4 846.52 1.73 505 182 223 41 117.97 0.06 506 182 224 42 117.98 0.07 507 182 225 43 117.89 0.07 508 182 226 44 117.64 0.19 509 183 186 3 864.77 1.27 510 183 187 4 855.19 6.07 511 184 187 3 864.77 1.28 512 184 188 4 855.19 6.07 513 185 188 3 855.03 5.33 514 185 189 4 852.75 4.84 515 185 206 21 203.38 0.93 516 185 207 22 203.37 0.93 517 185 208 23 203.33 0.93 518 185 225 40 117.17 0.27 519 185 226 41 116.91 0.11 520 186 189 3 864.08 0.81 521 186 190 4 850.83 5.81 522 186 225 39 117.33 0.24 523 187 190 3 866.46 0.38 524 187 191 4 851.50 6.17 525 188 191 3 858.23 2.81 526 188 192 4 851.60 6.12 527 188 206 18 203.47 0.93 528 189 192 3 856.68 4.49 529 189 193 4 841.38 10.52 530 189 201 12 204.56 0.47 531 189 204 15 203.91 0.78 532 189 206 17 203.51 0.92 533 189 225 36 117.12 0.25 534 189 227 38 117.09 0.24 535 190 193 3 859.17 2.33 536 190 194 4 852.76 4.83 537 191 194 3 860.03 1.19 538 191 195 4 837.50 2.79 539 192 195 3 853.43 5.16 540 192 196 4 825.69 3.12 541 192 197 5 824.12 3.06 542 192 201 9 204.55 0.46 543 192 204 12 203.75 0.84 544 193 196 3 852.96 2.62 545 193 197 4 790.26 0.29 546 193 198 5 463.10 41.80 547 193 201 8 234.09 12.57 548 196 200 4 825.90 2.13 549 197 200 3 866.99 2.16 550 197 201 4 840.88 4.11 551 198 201 3 854.44 2.49 552 198 202 4 840.89 4.11 553 199 202 3 861.51 9.03 554 199 203 4 846.12 6.65 555 200 203 3 855.25 2.69 556 200 204 4 840.95 4.07 557 201 204 3 863.83 2.72 558 205 227 22 115.93 0.03 559 206 225 19 116.74 0.10 560 206 226 20 116.62 0.15 561 206 227 21 116.40 0.14 562 207 227 20 116.03 0.05 563 207 228 21 115.66 0.04 564 207 229 22 114.96 0.30 565 208 212 4 884.53 1.01 566 208 220 12 117.92 0.48 567 208 226 18 116.66 0.14 568 208 228 20 116.03 0.05 569 208 229 21 115.67 0.05 570 209 230 21 114.50 0.07 571 211 216 5 817.86 2.21 572 212 216 4 817.86 2.21 573 212 217 5 417.94 69.28 574 212 220 8 256.48 1.98 575 212 229 17 116.20 0.12 576 212 231 19 115.99 0.06 577 212 234 22 115.03 0.34 578 212 243 31 50.69 0.49 579 213 216 3 870.10 3.27 580 213 217 4 817.85 2.21 581 213 234 21 115.69 0.01 582 213 243 30 50.70 0.49 583 214 217 3 871.29 2.68 584 214 239 25 54.70 2.44 585 214 242 28 50.69 0.49 586 214 243 29 50.69 0.49 587 216 219 3 862.78 0.70 588 216 220 4 846.26 2.67 589 217 220 3 852.97 0.68 590 217 221 4 839.27 2.12 591 217 229 12 117.54 0.04 592 217 243 26 45.52 0.44 593 217 246 29 33.54 0.12 594 218 221 3 872.00 4.75 595 218 222 4 843.31 4.15 596 218 246 28 31.91 0.69 597 219 222 3 856.98 2.76 598 219 223 4 851.79 5.36 599 220 223 3 863.70 0.60 600 220 226 6 121.34 1.01 601 220 229 9 117.83 0.17 602 221 226 5 120.16 0.42 603 221 227 6 119.49 0.09 604 221 228 7 118.02 0.14 605 221 229 8 118.02 0.14 606 221 246 25 31.64 0.59 607 221 247 26 29.22 1.42 608 223 226 3 844.55 4.09 609 229 243 14 43.95 0.61 610 229 246 17 32.60 0.15 611 231 234 3 895.46 1.79 612 231 235 4 843.31 4.27 613 231 240 9 84.23 17.21 614 231 243 12 50.10 0.53 615 232 235 3 874.66 3.47 616 232 236 4 857.27 2.48 617 233 236 3 873.08 2.68 618 234 237 3 860.08 2.60 619 234 240 6 199.66 34.83 620 234 243 9 146.09 48.15 621 235 240 5 267.62 68.81 622 237 240 3 869.98 0.60 623 238 241 3 893.65 4.67 624 239 242 3 863.52 0.67 625 240 243 3 851.73 1.32 626 240 244 4 829.99 2.45 627 241 244 3 845.41 0.49 628 241 245 4 829.63 2.63 629 242 245 3 844.47 4.41 630 242 246 4 829.98 2.46 631 243 246 3 852.27 0.51 632 244 247 3 868.71 1.39 633 244 248 4 862.66 4.40 634 245 248 3 862.66 4.40