BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 673 676 3 848.86 4.45 2 673 677 4 838.55 0.70 3 673 723 50 110.03 42.47 4 673 806 133 109.94 42.52 5 673 834 161 109.88 42.55 6 673 837 164 109.88 42.55 7 674 677 3 838.56 0.70 8 674 678 4 837.16 1.40 9 674 802 128 110.01 42.49 10 674 806 132 109.94 42.52 11 674 841 167 110.05 42.54 12 675 678 3 838.55 0.70 13 675 679 4 820.62 0.18 14 676 679 3 837.15 1.40 15 676 680 4 819.64 0.32 16 676 723 47 114.06 41.99 17 677 680 3 867.89 4.90 18 677 681 4 819.62 0.30 19 677 722 45 116.37 42.59 20 677 723 46 117.69 43.27 21 677 805 128 113.79 42.11 22 677 806 129 113.80 42.11 23 678 681 3 838.84 0.59 24 678 802 124 114.78 42.59 25 678 805 127 113.81 42.11 26 680 718 38 118.61 43.49 27 680 721 41 118.69 43.52 28 680 722 42 118.67 43.52 29 681 718 37 118.71 43.53 30 681 804 123 118.61 43.51 31 681 805 124 118.59 43.51 32 681 808 127 118.59 43.50 33 682 714 32 121.66 42.61 34 682 718 36 118.73 43.52 35 684 766 82 254.91 86.06 36 685 707 22 254.72 86.02 37 685 710 25 252.83 86.23 38 685 711 26 247.80 86.70 39 685 714 29 247.80 86.71 40 685 765 80 254.78 86.02 41 685 766 81 254.99 86.03 42 686 707 21 254.88 85.98 43 686 710 24 254.84 86.00 44 686 765 79 254.91 86.06 45 686 766 80 254.99 86.03 46 686 767 81 254.99 86.03 47 687 703 16 254.97 86.03 48 687 706 19 254.96 86.02 49 687 707 20 254.88 85.98 50 687 710 23 254.89 85.98 51 687 765 78 254.99 86.03 52 687 767 80 255.33 85.89 53 687 768 81 255.02 86.03 54 688 710 22 255.34 85.86 55 688 767 79 255.49 85.85 56 689 695 6 749.46 8.46 57 689 699 10 255.56 85.83 58 689 700 11 255.54 85.84 59 689 703 14 255.49 85.84 60 689 767 78 255.51 85.86 61 689 768 79 255.49 85.85 62 689 774 85 375.74 26.42 63 690 693 3 850.34 1.27 64 691 767 76 255.64 85.86 65 691 774 83 439.43 1.51 66 695 700 5 789.91 5.57 67 695 773 78 490.94 0.24 68 695 774 79 487.28 1.99 69 696 699 3 837.82 0.99 70 696 700 4 816.11 7.27 71 696 777 81 496.10 2.27 72 697 700 3 841.24 1.55 73 697 701 4 819.83 9.14 74 697 777 80 496.12 2.27 75 697 778 81 491.04 0.27 76 697 779 82 496.12 2.27 77 697 880 183 165.28 8.10 78 697 887 190 137.77 7.03 79 697 889 192 129.50 11.16 80 698 701 3 841.25 1.55 81 698 702 4 836.23 4.07 82 698 887 189 137.77 7.03 83 698 888 190 129.48 11.18 84 698 889 191 127.70 12.04 85 699 702 3 841.25 1.56 86 699 703 4 819.23 5.72 87 700 703 3 846.57 5.49 88 700 704 4 814.27 2.12 89 700 770 70 501.22 2.46 90 700 774 74 501.81 2.31 91 700 777 77 502.19 2.48 92 700 778 78 501.82 2.31 93 701 704 3 828.95 3.33 94 701 705 4 814.27 2.12 95 701 778 77 502.19 2.48 96 701 780 79 501.88 2.33 97 701 876 175 195.93 4.07 98 701 880 179 180.09 3.80 99 701 889 188 141.91 22.65 100 702 705 3 839.55 1.51 101 702 706 4 831.30 2.19 102 702 889 187 140.91 23.16 103 703 706 3 837.89 1.10 104 703 707 4 825.98 4.86 105 703 768 65 504.41 1.86 106 704 707 3 838.16 1.23 107 704 708 4 825.98 4.86 108 704 764 60 504.25 1.80 109 704 768 64 506.04 1.32 110 704 770 66 506.75 1.15 111 704 780 76 504.24 2.38 112 705 708 3 825.99 4.86 113 705 709 4 825.99 4.86 114 705 889 184 141.03 23.21 115 705 890 185 140.64 23.41 116 705 891 186 140.25 23.50 117 705 892 187 140.21 23.49 118 705 895 190 140.00 23.39 119 706 709 3 838.59 1.02 120 706 710 4 837.36 0.83 121 706 892 186 140.21 23.49 122 707 710 3 837.14 0.95 123 707 711 4 837.14 0.95 124 707 764 57 507.08 1.24 125 707 765 58 507.66 1.41 126 707 768 61 507.72 1.43 127 708 711 3 844.13 4.44 128 708 712 4 837.13 0.94 129 708 741 33 506.06 1.30 130 708 745 37 505.95 1.35 131 709 712 3 836.10 0.43 132 709 713 4 833.99 1.49 133 709 892 183 140.31 23.55 134 709 893 184 140.31 23.55 135 709 894 185 140.12 23.55 136 709 895 186 139.77 23.53 137 710 713 3 853.47 3.33 138 710 714 4 832.46 0.72 139 711 714 3 839.40 0.06 140 711 715 4 826.62 2.20 141 711 745 34 507.62 1.34 142 712 715 3 837.50 3.24 143 712 716 4 826.62 2.21 144 712 741 29 507.69 1.28 145 712 894 182 140.21 23.59 146 712 898 186 139.76 23.52 147 713 716 3 839.86 2.06 148 713 717 4 834.84 0.46 149 714 717 3 842.32 0.82 150 714 718 4 826.73 2.22 151 715 718 3 842.32 0.83 152 715 719 4 826.73 2.23 153 715 737 22 512.90 1.43 154 715 744 29 512.90 1.43 155 716 719 3 839.95 2.00 156 716 720 4 826.75 2.23 157 716 734 18 513.74 1.60 158 716 737 21 512.91 1.42 159 717 720 3 845.83 1.41 160 717 721 4 824.93 3.13 161 718 721 3 834.11 0.92 162 718 722 4 824.91 3.12 163 718 744 26 514.77 1.88 164 718 808 90 514.75 1.88 165 718 809 91 514.58 1.93 166 718 812 94 514.56 1.93 167 719 722 3 829.18 0.99 168 719 725 6 635.39 27.03 169 719 737 18 514.63 1.94 170 719 812 93 514.56 1.93 171 720 725 5 625.87 31.80 172 720 726 6 615.59 36.93 173 720 733 13 514.84 1.88 174 720 734 14 514.86 1.90 175 720 737 17 514.85 1.89 176 722 725 3 829.44 1.75 177 722 809 87 514.82 1.86 178 722 812 90 514.82 1.86 179 722 834 112 280.48 5.50 180 723 830 107 301.43 15.86 181 723 834 111 280.50 5.50 182 724 727 3 839.82 2.04 183 724 830 106 301.41 15.87 184 725 728 3 847.69 3.54 185 725 733 8 565.90 2.30 186 725 736 11 553.02 4.12 187 725 737 12 540.03 10.62 188 725 812 87 514.90 1.83 189 725 813 88 514.86 1.84 190 725 830 105 301.40 15.87 191 726 733 7 743.27 4.62 192 727 826 99 331.57 5.12 193 728 732 4 816.53 2.21 194 728 733 5 809.39 3.07 195 728 813 85 514.89 1.86 196 728 826 98 337.48 2.06 197 728 827 99 339.12 1.05 198 728 830 102 320.55 8.77 199 729 732 3 834.44 9.30 200 729 733 4 820.53 0.52 201 729 822 93 347.01 1.45 202 729 823 94 346.98 1.44 203 730 733 3 844.63 4.21 204 730 734 4 831.06 5.78 205 731 734 3 853.92 0.43 206 731 735 4 820.54 0.53 207 731 822 91 347.02 1.45 208 732 735 3 820.55 0.54 209 732 736 4 823.35 1.94 210 732 821 89 347.04 1.45 211 732 822 90 347.01 1.45 212 732 823 91 346.99 1.44 213 733 736 3 851.16 1.08 214 733 737 4 825.12 4.20 215 734 737 3 840.20 3.16 216 734 738 4 825.15 4.19 217 735 738 3 840.20 3.16 218 735 739 4 825.75 0.70 219 735 821 86 347.04 1.45 220 736 739 3 840.19 3.16 221 736 740 4 834.46 0.47 222 736 812 76 515.26 1.97 223 736 816 80 489.51 8.18 224 736 821 85 347.04 1.46 225 736 827 91 296.76 12.96 226 737 740 3 842.06 3.33 227 737 741 4 807.22 7.09 228 737 812 75 515.26 1.98 229 738 741 3 849.11 7.78 230 738 897 159 140.02 23.68 231 738 898 160 139.49 23.67 232 738 902 164 127.73 25.92 233 739 815 76 514.70 1.71 234 739 817 78 514.65 1.69 235 739 902 163 127.71 25.93 236 739 905 166 124.33 26.77 237 739 906 167 123.77 26.83 238 739 911 172 97.15 28.05 239 740 743 3 823.05 2.07 240 740 744 4 809.02 3.96 241 740 811 71 515.38 1.97 242 740 812 72 515.37 1.98 243 740 815 75 514.65 1.69 244 741 744 3 836.83 4.82 245 741 745 4 825.05 4.04 246 741 895 154 140.08 23.64 247 741 898 157 139.65 23.51 248 742 745 3 835.86 0.97 249 742 746 4 825.64 3.75 250 742 870 128 217.36 4.55 251 742 873 131 215.28 5.50 252 742 874 132 215.18 5.54 253 742 877 135 201.10 12.32 254 743 746 3 835.39 0.06 255 743 747 4 825.20 3.97 256 743 811 68 516.01 2.01 257 743 866 123 247.50 12.26 258 743 870 127 217.34 4.57 259 744 747 3 840.07 3.46 260 744 748 4 820.18 1.60 261 744 808 64 516.01 2.01 262 744 811 67 516.01 2.02 263 744 812 68 516.00 2.02 264 745 748 3 839.18 1.95 265 745 749 4 810.01 4.54 266 746 749 3 843.62 4.17 267 746 750 4 810.18 4.50 268 746 787 41 537.07 1.54 269 746 790 44 537.06 1.54 270 746 866 120 247.49 12.27 271 746 869 123 221.59 2.50 272 746 873 127 215.93 5.17 273 747 750 3 835.87 0.60 274 747 751 4 821.10 5.98 275 747 790 43 537.07 1.54 276 747 794 47 537.06 1.54 277 747 804 57 516.26 2.05 278 747 807 60 516.16 2.06 279 747 808 61 516.13 2.05 280 747 811 64 516.11 2.04 281 747 862 115 255.93 10.03 282 747 863 116 255.77 9.96 283 747 866 119 247.50 12.26 284 748 751 3 835.11 1.00 285 748 752 4 822.59 5.24 286 748 804 56 516.22 2.05 287 748 808 60 516.12 2.05 288 749 752 3 837.93 2.44 289 749 753 4 821.10 5.98 290 749 762 13 540.92 1.33 291 749 763 14 540.83 1.34 292 749 764 15 540.52 1.45 293 749 787 38 537.28 1.45 294 750 753 3 840.48 2.48 295 750 754 4 824.39 4.33 296 750 791 41 537.23 1.46 297 750 794 44 537.25 1.45 298 751 754 3 839.87 3.41 299 751 755 4 817.73 1.00 300 751 794 43 537.23 1.46 301 751 799 48 516.67 2.03 302 751 800 49 516.63 2.04 303 751 801 50 516.45 2.08 304 751 804 53 516.22 2.05 305 752 755 3 838.08 4.30 306 752 756 4 817.73 1.01 307 752 763 11 540.93 1.33 308 752 764 12 540.92 1.34 309 753 756 3 833.21 6.73 310 753 757 4 810.55 4.59 311 753 758 5 740.33 15.38 312 753 759 6 605.13 21.30 313 753 761 8 541.39 1.54 314 753 762 9 541.02 1.37 315 753 763 10 540.91 1.33 316 753 791 38 537.37 1.49 317 754 758 4 673.81 1.72 318 754 759 5 594.07 15.77 319 754 791 37 537.39 1.50 320 754 794 40 537.29 1.47 321 754 795 41 537.26 1.46 322 756 763 7 541.07 1.38 323 757 760 3 863.25 2.50 324 757 761 4 784.54 3.89 325 757 763 6 543.96 1.50 326 759 788 29 537.71 1.60 327 759 791 32 537.55 1.54 328 759 792 33 537.51 1.55 329 760 788 28 537.91 1.53 330 761 769 8 541.75 0.83 331 761 770 9 540.40 1.42 332 761 771 10 540.37 1.44 333 761 775 14 540.40 1.42 334 762 769 7 541.68 0.79 335 762 770 8 540.37 1.43 336 762 775 13 540.34 1.43 337 762 784 22 538.14 1.62 338 762 787 25 537.89 1.52 339 762 788 26 537.55 1.52 340 763 766 3 888.63 0.70 341 763 769 6 543.02 1.46 342 764 768 4 807.51 5.24 343 764 770 6 650.83 38.12 344 764 787 23 538.15 1.65 345 765 768 3 821.58 1.48 346 765 769 4 807.53 5.25 347 766 769 3 873.96 3.90 348 768 774 6 804.11 2.62 349 769 774 5 813.70 2.18 350 770 774 4 804.11 2.62 351 770 775 5 804.10 2.62 352 770 778 8 770.59 3.06 353 770 780 10 719.95 7.92 354 770 784 14 541.83 1.41 355 771 774 3 850.15 0.54 356 771 775 4 820.15 1.81 357 772 775 3 833.88 8.67 358 772 776 4 837.80 6.71 359 772 781 9 704.77 0.35 360 772 788 16 546.13 1.28 361 773 776 3 850.06 0.58 362 773 777 4 836.49 7.36 363 774 777 3 823.05 0.69 364 774 778 4 815.53 3.12 365 775 778 3 848.80 0.12 366 775 779 4 827.47 2.90 367 775 780 5 767.81 13.66 368 775 781 6 704.76 0.35 369 775 784 9 549.06 1.14 370 775 788 13 547.45 1.94 371 776 779 3 828.05 3.19 372 776 781 5 704.78 0.34 373 779 876 97 196.32 4.27 374 780 783 3 840.41 7.94 375 780 784 4 807.90 0.43 376 780 876 96 196.28 4.26 377 781 784 3 831.22 8.90 378 781 785 4 811.09 1.16 379 782 785 3 834.76 8.22 380 782 786 4 816.96 3.52 381 783 786 3 847.20 2.00 382 783 787 4 818.75 2.62 383 783 872 89 213.16 2.47 384 783 873 90 212.26 2.02 385 783 876 93 210.16 2.67 386 784 787 3 830.63 3.48 387 784 788 4 818.79 2.65 388 785 788 3 823.93 0.12 389 785 789 4 825.73 0.79 390 786 789 3 840.13 5.91 391 786 790 4 828.95 0.83 392 786 868 82 224.73 0.94 393 786 869 83 221.55 2.43 394 787 790 3 835.77 4.24 395 787 791 4 830.78 1.75 396 787 869 82 221.50 2.46 397 787 873 86 217.23 4.51 398 788 791 3 835.77 4.25 399 788 792 4 830.77 1.75 400 789 792 3 832.13 1.07 401 789 793 4 826.17 4.05 402 789 796 7 802.82 2.93 403 790 793 3 847.00 6.37 404 790 794 4 827.52 3.38 405 790 862 72 255.77 9.96 406 790 866 76 248.81 11.61 407 790 869 79 223.81 1.33 408 791 794 3 848.53 5.59 409 792 795 3 842.58 6.91 410 792 796 4 819.97 1.83 411 793 796 3 825.79 4.49 412 793 797 4 809.42 3.43 413 793 862 69 255.84 10.00 414 794 798 4 792.27 0.90 415 794 799 5 640.95 16.50 416 794 804 10 526.28 6.96 417 794 862 68 256.00 10.08 418 795 798 3 825.50 4.63 419 797 855 58 256.46 10.10 420 797 858 61 256.12 10.11 421 797 862 65 256.08 10.11 422 798 855 57 256.55 10.08 423 799 803 4 823.95 2.21 424 799 804 5 808.19 0.10 425 799 807 8 778.47 5.13 426 799 855 56 256.49 10.11 427 799 859 60 256.11 10.11 428 799 862 63 256.09 10.11 429 800 803 3 838.21 4.87 430 800 804 4 818.34 5.05 431 801 804 3 828.99 0.27 432 801 805 4 826.16 1.15 433 802 805 3 831.28 1.40 434 802 806 4 828.46 0.00 435 802 841 39 263.37 11.46 436 803 806 3 837.60 2.75 437 803 807 4 826.15 1.15 438 803 841 38 261.68 10.65 439 803 855 52 256.52 10.12 440 803 859 56 256.13 10.10 441 804 807 3 838.82 5.17 442 804 808 4 826.15 1.16 443 805 808 3 848.45 0.35 444 805 809 4 823.84 3.89 445 806 809 3 841.77 3.69 446 806 810 4 819.10 2.00 447 806 838 32 267.10 9.62 448 806 841 35 261.77 10.62 449 807 810 3 845.27 2.37 450 807 811 4 825.00 3.41 451 807 838 31 272.27 7.04 452 807 859 52 256.13 10.10 453 807 862 55 256.12 10.09 454 807 863 56 256.01 10.07 455 808 811 3 840.92 4.55 456 808 812 4 826.42 2.70 457 809 812 3 852.59 1.28 458 809 813 4 839.77 2.69 459 809 834 25 279.36 5.38 460 810 813 3 838.13 4.71 461 810 814 4 825.04 3.40 462 810 831 21 280.07 5.26 463 810 834 24 279.35 5.38 464 810 835 25 279.03 5.48 465 810 838 28 273.67 7.45 466 810 863 53 256.01 10.07 467 810 916 106 87.90 28.24 468 811 814 3 821.53 0.26 469 811 863 52 255.90 10.02 470 811 866 55 255.27 10.18 471 811 867 56 255.25 10.18 472 813 827 14 295.68 13.02 473 813 830 17 280.97 5.68 474 813 831 18 280.07 5.25 475 813 834 21 279.97 5.23 476 813 914 101 88.53 28.25 477 814 831 17 280.80 5.59 478 814 867 53 255.30 10.21 479 814 913 99 88.56 28.24 480 814 914 100 88.16 28.07 481 815 906 91 123.78 26.84 482 815 911 96 97.88 28.41 483 815 912 97 94.70 29.09 484 815 913 98 88.56 28.23 485 815 914 99 88.55 28.23 486 816 821 5 598.73 16.86 487 816 824 8 346.90 1.40 488 816 827 11 344.56 2.00 489 816 911 95 98.73 28.84 490 816 912 96 97.83 28.42 491 816 914 98 93.64 29.30 492 817 820 3 848.19 0.24 493 817 821 4 793.41 3.51 494 817 910 93 106.81 31.36 495 817 911 94 99.06 29.00 496 818 910 92 109.02 32.46 497 818 911 93 106.81 31.37 498 818 912 94 106.67 31.35 499 820 824 4 801.73 10.78 500 821 824 3 813.70 4.79 501 821 827 6 775.65 1.80 502 823 826 3 856.20 4.15 503 823 827 4 835.99 2.19 504 824 827 3 848.69 6.87 505 824 828 4 834.90 1.66 506 825 828 3 850.25 3.80 507 825 829 4 834.90 1.66 508 826 829 3 841.55 0.56 509 826 830 4 834.90 1.66 510 827 830 3 845.69 1.51 511 827 831 4 821.62 3.64 512 827 914 87 93.39 30.05 513 828 831 3 840.93 0.71 514 828 832 4 821.62 3.65 515 829 832 3 843.65 2.07 516 829 833 4 826.81 1.04 517 830 833 3 843.65 2.07 518 830 834 4 826.71 0.99 519 831 834 3 838.68 4.54 520 831 835 4 819.31 4.69 521 831 914 83 92.48 30.23 522 831 916 85 92.08 30.18 523 832 835 3 842.90 2.42 524 832 836 4 828.68 0.85 525 833 836 3 839.30 4.46 526 833 837 4 828.68 0.85 527 834 837 3 828.69 0.85 528 834 838 4 828.68 0.86 529 835 838 3 849.42 2.17 530 835 839 4 828.66 0.86 531 835 856 21 256.49 10.10 532 835 916 81 84.72 29.94 533 836 839 3 820.98 2.98 534 836 840 4 820.98 2.98 535 837 840 3 839.11 0.23 536 837 841 4 819.45 2.21 537 838 841 3 832.58 2.88 538 838 842 4 831.10 3.61 539 838 856 18 256.50 10.10 540 838 859 21 256.50 10.10 541 839 842 3 841.82 0.38 542 839 843 4 819.44 2.21 543 840 843 3 830.86 3.26 544 840 844 4 805.40 9.06 545 841 844 3 847.38 0.82 546 842 847 5 593.47 9.81 547 842 855 13 256.64 10.10 548 842 856 14 256.63 10.10 549 842 859 17 256.62 10.10 550 843 847 4 656.32 28.30 551 847 852 5 256.83 10.16 552 847 855 8 256.77 10.16 553 852 855 3 863.67 1.50 554 852 856 4 831.27 0.87 555 853 856 3 844.88 3.90 556 853 857 4 803.84 8.12 557 854 857 3 830.31 1.35 558 854 858 4 826.54 3.23 559 855 858 3 839.81 1.16 560 855 859 4 824.01 1.36 561 856 859 3 852.34 4.19 562 856 860 4 824.01 1.36 563 857 860 3 851.64 2.90 564 857 861 4 823.62 1.55 565 857 917 60 83.80 30.14 566 858 861 3 824.84 0.94 567 858 862 4 822.93 1.22 568 859 862 3 825.32 1.18 569 859 863 4 832.43 2.38 570 860 863 3 843.62 1.18 571 860 864 4 837.23 0.03 572 860 916 56 83.74 30.12 573 860 917 57 83.74 30.12 574 861 864 3 841.25 0.01 575 861 865 4 837.24 0.03 576 861 917 56 83.72 30.03 577 862 865 3 847.26 5.04 578 862 866 4 842.60 0.70 579 863 866 3 849.31 4.04 580 864 867 3 855.72 0.83 581 864 868 4 852.36 2.51 582 864 915 51 85.90 29.07 583 864 916 52 85.62 29.18 584 864 917 53 83.84 29.89 585 865 868 3 865.62 2.25 586 865 869 4 848.52 3.66 587 866 869 3 850.35 2.75 588 866 870 4 836.02 2.99 589 867 870 3 841.45 0.29 590 867 871 4 836.02 2.99 591 867 913 46 89.58 27.24 592 867 915 48 88.02 27.98 593 868 871 3 836.03 2.99 594 868 872 4 841.44 0.29 595 868 915 47 89.58 27.23 596 869 872 3 841.74 0.43 597 869 873 4 834.39 3.24 598 870 873 3 821.15 3.38 599 870 874 4 816.09 5.90 600 871 874 3 824.45 1.72 601 871 875 4 821.33 3.27 602 871 906 35 122.95 26.07 603 871 907 36 121.14 25.30 604 872 875 3 837.58 2.81 605 872 876 4 821.18 3.19 606 873 876 3 833.92 3.18 607 873 877 4 810.34 2.75 608 874 877 3 849.25 3.54 609 874 878 4 810.38 2.76 610 874 903 29 126.94 25.46 611 874 906 32 122.97 26.07 612 874 907 33 121.28 25.24 613 875 878 3 838.17 1.80 614 875 879 4 810.38 2.76 615 875 907 32 121.28 25.23 616 876 879 3 841.31 0.26 617 876 880 4 810.54 2.68 618 877 880 3 829.22 4.55 619 877 881 4 818.00 1.06 620 877 899 22 138.59 22.92 621 878 881 3 849.25 4.22 622 878 882 4 826.90 2.90 623 878 899 21 138.62 22.93 624 878 903 25 131.09 23.39 625 878 904 26 129.78 24.05 626 878 907 29 122.28 24.77 627 879 882 3 837.77 1.25 628 879 883 4 838.47 1.59 629 880 883 3 841.36 1.28 630 880 884 4 818.16 1.06 631 880 887 7 734.19 8.41 632 880 891 11 141.48 23.05 633 881 884 3 839.62 2.16 634 881 885 4 852.78 3.98 635 881 891 10 141.35 23.11 636 881 896 15 140.11 23.34 637 881 899 18 138.86 22.82 638 881 900 19 138.80 22.80 639 882 885 3 913.43 0.53 640 883 886 3 845.89 0.20 641 883 887 4 837.58 2.02 642 884 887 3 838.24 3.58 643 884 888 4 817.88 3.64 644 884 889 5 207.54 51.33 645 884 891 7 146.21 20.68 646 891 895 4 809.19 2.68 647 891 896 5 734.98 7.16 648 891 899 8 713.09 18.07 649 892 895 3 860.68 1.29 650 892 896 4 809.19 2.68 651 893 896 3 854.02 4.62 652 893 897 4 823.03 3.14 653 894 897 3 854.02 4.62 654 894 898 4 829.83 0.27 655 895 898 3 831.14 0.19 656 895 899 4 822.53 3.92 657 896 899 3 836.91 3.27 658 896 900 4 828.80 0.79 659 897 900 3 836.91 3.27 660 897 901 4 802.46 13.94 661 898 901 3 829.80 0.27 662 898 902 4 743.23 4.46 663 898 903 5 733.40 9.38 664 899 903 4 805.46 3.36 665 899 904 5 819.06 3.45 666 900 904 4 823.85 4.16 667 901 904 3 868.60 4.05 668 901 905 4 844.50 0.70 669 902 905 3 853.21 3.64 670 902 906 4 844.52 0.71 671 903 906 3 854.50 4.28 672 903 907 4 844.51 0.70 673 904 907 3 842.47 1.74 674 904 908 4 830.75 7.57 675 905 908 3 842.32 1.81 676 905 909 4 830.77 7.58 677 905 910 5 627.03 9.33 678 905 911 6 565.32 14.30 679 906 909 3 844.39 0.74 680 906 911 5 429.49 82.22 681 909 912 3 869.93 0.99