BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 202 199 176.59 1.44 2 4 189 185 174.94 0.60 3 4 202 198 176.59 1.44 4 4 203 199 174.93 0.61 5 4 204 200 174.91 0.61 6 4 205 201 172.74 0.24 7 4 224 220 119.42 0.35 8 4 227 223 119.12 0.25 9 5 35 30 821.80 1.76 10 5 36 31 821.77 1.77 11 5 37 32 821.75 1.77 12 5 38 33 821.72 1.78 13 5 205 200 175.26 0.79 14 6 36 30 821.79 1.76 15 6 37 31 821.76 1.77 16 6 38 32 821.75 1.77 17 6 221 215 119.29 0.28 18 6 227 221 119.47 0.37 19 6 228 222 119.29 0.29 20 6 229 223 119.30 0.29 21 6 243 237 48.59 1.06 22 6 246 240 41.55 0.29 23 6 247 241 31.95 0.04 24 7 38 31 821.76 1.77 25 7 40 33 821.75 1.77 26 7 90 83 478.34 58.87 27 7 124 117 403.13 28.77 28 7 161 154 330.62 0.55 29 7 205 198 181.03 0.60 30 7 207 200 175.27 0.82 31 7 227 220 119.59 0.39 32 7 228 221 119.34 0.30 33 7 229 222 119.30 0.29 34 8 37 29 821.81 1.76 35 8 38 30 821.78 1.77 36 8 39 31 821.76 1.77 37 8 40 32 821.76 1.77 38 8 229 221 119.48 0.36 39 8 240 232 61.50 0.56 40 8 243 235 48.04 1.40 41 8 244 236 43.33 0.73 42 9 40 31 821.76 1.77 43 9 229 220 120.20 0.57 44 9 230 221 119.30 0.29 45 9 231 222 119.29 0.28 46 9 240 231 61.62 0.55 47 10 40 30 821.79 1.77 48 10 41 31 821.76 1.77 49 10 42 32 821.75 1.77 50 10 231 221 119.30 0.29 51 10 235 225 84.17 0.97 52 10 240 230 61.62 0.55 53 11 42 31 821.76 1.77 54 11 64 53 527.71 83.36 55 11 95 84 440.18 40.97 56 11 230 219 121.15 0.33 57 11 231 220 119.67 0.44 58 11 232 221 119.30 0.29 59 11 235 224 89.55 1.67 60 12 15 3 855.93 2.41 61 12 21 9 822.69 1.79 62 12 24 12 822.18 1.67 63 12 25 13 822.18 1.67 64 12 41 29 821.81 1.77 65 12 42 30 821.78 1.77 66 12 43 31 821.77 1.77 67 12 44 32 821.75 1.77 68 12 51 39 696.64 11.64 69 12 55 43 634.40 32.32 70 12 64 52 528.02 83.22 71 12 235 223 109.05 1.43 72 12 236 224 88.06 0.93 73 13 44 31 821.77 1.77 74 13 64 51 527.86 83.14 75 13 95 82 474.50 56.71 76 13 97 84 430.94 35.17 77 13 232 219 121.16 0.33 78 14 44 30 821.78 1.77 79 15 21 6 825.34 1.60 80 15 44 29 821.80 1.77 81 15 236 221 119.19 0.25 82 16 20 4 829.35 3.60 83 16 21 5 825.34 1.60 84 16 47 31 821.73 1.75 85 17 20 3 851.11 1.87 86 17 21 4 837.34 3.26 87 17 47 30 821.72 1.76 88 18 21 3 850.66 2.09 89 18 22 4 837.42 3.22 90 18 47 29 821.65 1.76 91 18 50 32 751.97 5.18 92 19 22 3 852.23 2.88 93 19 23 4 830.44 1.23 94 20 23 3 845.05 0.03 95 20 24 4 843.08 1.34 96 21 24 3 847.08 0.66 97 21 25 4 847.12 0.67 98 21 44 23 821.81 1.76 99 21 47 26 820.83 1.89 100 21 50 29 731.48 4.34 101 21 236 215 111.16 2.56 102 22 25 3 856.57 0.96 103 22 26 4 847.49 0.85 104 22 50 28 731.43 4.32 105 23 26 3 847.50 0.85 106 23 27 4 847.53 0.87 107 24 27 3 856.75 3.67 108 24 28 4 847.52 0.88 109 24 235 211 116.81 0.23 110 24 236 212 94.66 1.50 111 25 28 3 852.37 1.54 112 25 29 4 842.40 3.43 113 25 50 25 719.27 0.95 114 25 51 26 685.79 6.21 115 25 54 29 531.50 82.93 116 25 55 30 531.40 82.91 117 26 29 3 848.02 0.62 118 26 30 4 842.38 3.43 119 27 30 3 843.48 2.89 120 27 31 4 840.63 4.30 121 27 238 211 73.87 3.88 122 27 241 214 62.12 0.74 123 28 31 3 838.58 5.33 124 28 41 13 821.82 1.77 125 28 58 30 530.38 83.20 126 28 59 31 530.04 83.27 127 28 240 212 62.08 0.73 128 28 241 213 61.54 0.57 129 28 244 216 56.61 0.38 130 29 54 25 531.49 82.94 131 29 55 26 531.34 82.92 132 29 56 27 530.54 83.23 133 31 58 27 530.34 83.20 134 31 241 210 61.16 0.47 135 32 58 26 530.08 83.27 136 33 37 4 827.07 0.50 137 33 39 6 822.06 1.84 138 33 58 25 528.81 83.22 139 33 244 211 47.74 1.54 140 34 37 3 835.20 2.54 141 34 244 210 45.62 0.30 142 34 245 211 44.85 0.43 143 34 248 214 35.35 0.07 144 36 248 212 32.44 0.17 145 37 244 207 44.02 0.85 146 38 90 52 478.59 58.83 147 38 205 167 182.34 0.15 148 39 59 20 528.34 83.12 149 39 60 21 528.33 83.12 150 39 240 201 61.59 0.55 151 39 244 205 43.32 0.73 152 40 59 19 528.55 83.22 153 40 60 20 528.33 83.12 154 40 61 21 528.32 83.12 155 40 62 22 528.19 83.17 156 40 90 50 478.88 58.76 157 40 124 84 426.53 33.74 158 41 55 14 530.52 83.24 159 41 59 18 528.62 83.23 160 41 60 19 528.56 83.22 161 41 61 20 528.33 83.12 162 41 62 21 528.26 83.15 163 41 240 199 61.69 0.57 164 42 62 20 528.24 83.16 165 42 64 22 527.97 83.23 166 42 92 50 478.09 58.56 167 43 47 4 824.34 0.91 168 43 48 5 769.55 24.97 169 43 51 8 684.98 6.87 170 43 61 18 528.61 83.23 171 43 62 19 528.55 83.22 172 43 64 21 528.19 83.17 173 44 47 3 855.20 10.48 174 44 48 4 829.44 2.39 175 44 64 20 528.30 83.20 176 45 48 3 870.23 2.96 177 45 78 33 479.49 59.63 178 47 50 3 834.05 1.31 179 47 51 4 831.39 2.65 180 48 51 3 857.32 0.40 181 48 52 4 837.84 5.84 182 48 61 13 528.84 83.22 183 48 63 15 528.56 83.22 184 48 78 30 480.60 59.08 185 48 86 38 480.34 58.39 186 48 88 40 480.23 58.33 187 49 52 3 847.30 1.12 188 49 53 4 836.63 0.12 189 49 86 37 480.25 58.40 190 50 53 3 847.28 1.11 191 50 54 4 837.23 0.17 192 51 54 3 842.37 0.30 193 51 55 4 836.61 0.12 194 51 61 10 529.03 83.12 195 52 55 3 840.41 1.28 196 52 56 4 860.56 1.97 197 52 57 5 831.35 2.71 198 52 59 7 631.09 33.08 199 52 60 8 530.42 83.23 200 52 61 9 529.04 83.12 201 52 86 34 480.48 58.28 202 52 87 35 480.42 58.25 203 55 59 4 777.70 22.87 204 55 61 6 561.16 67.77 205 56 59 3 873.06 1.52 206 60 89 29 509.75 43.52 207 60 90 30 509.68 43.50 208 61 88 27 509.85 43.50 209 61 89 28 509.78 43.52 210 61 90 29 509.69 43.49 211 62 88 26 509.96 43.45 212 62 90 28 509.68 43.48 213 62 91 29 509.56 43.42 214 62 124 62 458.58 17.94 215 63 78 15 511.66 43.55 216 63 83 20 510.29 43.41 217 63 90 27 509.85 43.49 218 63 91 28 509.62 43.38 219 63 92 29 509.62 43.38 220 64 92 28 509.49 43.32 221 65 78 13 511.88 43.45 222 65 83 18 511.51 43.46 223 65 91 26 510.11 43.35 224 65 92 27 509.76 43.33 225 65 93 28 509.70 43.34 226 65 98 33 462.69 19.84 227 65 104 39 462.32 19.43 228 65 112 47 462.00 19.14 229 66 78 12 511.85 43.47 230 66 80 14 511.60 43.52 231 66 83 17 511.58 43.45 232 66 91 25 510.19 43.34 233 66 93 27 510.04 43.30 234 66 112 46 462.07 19.13 235 66 116 50 461.67 18.80 236 67 74 7 512.10 43.47 237 67 77 10 512.03 43.47 238 67 78 11 511.94 43.45 239 67 80 13 511.93 43.45 240 67 108 41 462.25 19.21 241 67 112 45 462.13 19.14 242 68 108 40 462.26 19.20 243 68 111 43 462.15 19.14 244 68 112 44 462.13 19.14 245 68 115 47 462.24 19.05 246 69 79 10 512.04 43.47 247 69 115 46 462.46 18.98 248 70 79 9 512.04 43.47 249 70 115 45 462.56 18.94 250 70 119 49 462.74 18.84 251 71 82 11 512.10 43.45 252 74 77 3 857.21 5.72 253 78 82 4 837.25 1.54 254 78 83 5 834.11 0.01 255 79 82 3 863.91 2.88 256 79 83 4 837.52 1.41 257 80 83 3 857.74 3.14 258 80 84 4 838.27 0.49 259 80 115 35 466.27 16.68 260 80 116 36 462.46 18.05 261 80 119 39 476.82 10.54 262 80 121 41 464.18 15.46 263 81 84 3 839.80 0.27 264 81 85 4 835.70 0.79 265 81 119 38 476.91 10.56 266 82 85 3 840.91 3.08 267 82 86 4 835.62 0.82 268 83 86 3 846.94 0.07 269 83 87 4 836.00 0.63 270 83 88 5 658.15 17.69 271 83 90 7 541.88 28.96 272 83 91 8 526.42 35.34 273 83 121 38 489.62 2.73 274 84 87 3 840.91 3.08 275 84 88 4 761.58 36.59 276 84 119 35 489.72 2.75 277 84 121 37 489.64 2.72 278 88 121 33 526.88 15.97 279 89 121 32 526.87 15.96 280 89 122 33 526.78 15.97 281 90 121 31 526.88 15.97 282 90 122 32 526.78 15.97 283 90 123 33 526.70 16.02 284 90 124 34 526.60 16.07 285 90 159 69 461.72 48.55 286 90 160 70 461.66 48.56 287 90 161 71 461.63 48.55 288 91 116 25 528.75 15.11 289 91 121 30 526.94 16.00 290 91 122 31 526.90 16.00 291 91 123 32 526.73 16.04 292 91 124 33 526.61 16.07 293 92 95 3 914.52 3.81 294 92 124 32 526.64 16.08 295 92 126 34 526.58 16.07 296 92 230 138 121.27 0.35 297 93 112 19 529.44 14.78 298 93 113 20 529.33 14.81 299 93 116 23 529.34 14.87 300 93 123 30 526.88 16.02 301 93 124 31 526.74 16.05 302 93 125 32 526.60 16.07 303 93 126 33 526.58 16.07 304 94 99 5 621.52 16.82 305 94 109 15 529.61 14.67 306 94 126 32 526.54 16.10 307 95 98 3 848.43 0.41 308 95 99 4 782.58 28.46 309 95 126 31 526.52 16.14 310 95 165 70 460.44 49.12 311 95 230 135 122.39 0.54 312 96 99 3 856.52 3.63 313 96 100 4 729.28 1.80 314 96 165 69 460.26 49.21 315 96 167 71 456.88 48.72 316 97 100 3 847.95 4.61 317 97 101 4 666.09 5.48 318 97 102 5 654.20 33.14 319 98 102 4 640.80 18.09 320 98 103 5 580.83 10.62 321 98 104 6 530.58 14.41 322 99 103 4 530.95 14.30 323 99 104 5 530.58 14.41 324 99 106 7 530.58 14.41 325 99 109 10 530.58 14.41 326 99 126 27 526.78 16.15 327 99 127 28 526.76 16.15 328 99 146 47 462.80 48.15 329 100 103 3 662.34 5.45 330 100 130 30 526.10 16.36 331 100 167 67 458.78 48.76 332 104 108 4 846.22 1.54 333 104 109 5 831.23 1.04 334 104 112 8 710.76 1.08 335 105 108 3 855.90 3.29 336 105 109 4 838.23 2.45 337 106 109 3 842.85 0.72 338 106 110 4 837.45 2.06 339 106 146 40 462.80 48.14 340 106 149 43 462.51 48.38 341 107 110 3 861.19 5.69 342 107 111 4 850.30 0.24 343 108 111 3 853.62 1.90 344 108 112 4 842.02 4.38 345 109 112 3 857.82 0.20 346 109 113 4 840.52 5.12 347 110 113 3 857.49 0.03 348 110 114 4 840.53 5.12 349 110 149 39 462.48 48.41 350 110 153 43 462.67 48.66 351 111 114 3 844.14 3.27 352 111 115 4 790.51 30.09 353 112 115 3 847.68 1.51 354 112 116 4 790.48 30.09 355 113 116 3 854.69 2.02 356 113 117 4 839.40 3.00 357 113 123 10 527.60 16.23 358 113 150 37 462.56 48.45 359 113 153 40 462.78 48.73 360 113 154 41 462.81 48.79 361 114 117 3 860.01 2.71 362 114 118 4 839.42 2.99 363 114 153 39 462.67 48.69 364 115 118 3 858.22 3.61 365 115 119 4 839.44 3.00 366 116 119 3 847.47 1.74 367 116 120 4 839.45 3.00 368 116 121 5 709.08 2.38 369 116 123 7 527.84 16.36 370 117 120 3 848.50 7.04 371 117 121 4 829.82 1.80 372 117 123 6 529.01 16.93 373 117 153 36 464.56 49.63 374 117 154 37 464.51 49.64 375 117 159 42 464.46 49.89 376 117 160 43 464.24 49.78 377 122 159 37 511.26 73.36 378 123 159 36 511.42 73.39 379 123 160 37 511.28 73.38 380 123 161 38 511.28 73.38 381 124 161 37 511.28 73.39 382 124 163 39 510.94 73.54 383 124 207 83 195.97 1.64 384 124 230 106 120.95 0.41 385 125 150 25 511.83 73.30 386 125 161 36 511.38 73.42 387 125 162 37 511.20 73.42 388 125 163 38 511.15 73.44 389 126 163 37 511.17 73.45 390 126 165 39 510.23 73.89 391 126 230 104 122.32 0.50 392 127 143 16 512.66 73.07 393 127 146 19 512.23 73.24 394 127 147 20 512.24 73.28 395 127 150 23 512.16 73.22 396 127 162 35 511.41 73.42 397 127 163 36 511.38 73.43 398 127 164 37 511.03 73.56 399 127 165 38 510.81 73.61 400 127 188 61 242.13 3.33 401 128 143 15 512.70 73.08 402 128 146 18 512.65 73.07 403 128 164 36 511.23 73.48 404 128 165 37 510.82 73.61 405 129 133 4 720.77 2.27 406 129 134 5 720.72 2.26 407 129 139 10 512.82 73.07 408 129 142 13 512.78 73.09 409 129 143 14 512.76 73.09 410 129 164 35 511.30 73.50 411 129 167 38 509.98 74.02 412 129 168 39 509.85 74.03 413 130 133 3 852.43 3.71 414 130 134 4 782.16 26.53 415 130 167 37 509.92 74.05 416 130 168 38 509.85 74.04 417 131 134 3 852.19 1.07 418 131 135 4 836.82 0.79 419 131 168 37 509.85 74.04 420 131 172 41 483.73 65.99 421 131 174 43 448.09 65.19 422 132 135 3 852.81 1.39 423 132 136 4 836.83 0.78 424 133 136 3 866.05 7.99 425 133 137 4 836.83 0.78 426 133 138 5 584.25 39.16 427 133 142 9 524.13 67.54 428 134 137 3 841.02 1.43 429 134 139 5 586.72 36.30 430 134 164 30 511.50 73.44 431 134 168 34 509.91 74.06 432 134 180 46 245.83 3.02 433 134 183 49 245.73 2.98 434 134 184 50 244.86 2.90 435 138 141 3 842.60 3.43 436 138 142 4 717.99 0.40 437 139 142 3 861.31 5.92 438 139 143 4 839.35 1.91 439 139 184 45 243.32 3.31 440 140 143 3 859.55 2.79 441 140 144 4 841.29 1.79 442 140 187 47 242.65 3.29 443 141 144 3 857.32 3.92 444 141 145 4 850.50 2.13 445 142 145 3 864.12 0.52 446 142 146 4 857.31 3.92 447 143 146 3 852.06 1.52 448 143 147 4 840.40 2.26 449 143 164 21 511.45 73.42 450 143 188 45 242.26 3.31 451 144 147 3 845.44 0.40 452 144 148 4 834.28 0.84 453 144 187 43 242.58 3.33 454 144 191 47 242.17 3.34 455 145 148 3 847.53 1.45 456 145 149 4 840.39 2.21 457 146 149 3 852.22 0.89 458 146 150 4 840.39 2.21 459 147 150 3 849.41 2.29 460 147 151 4 840.38 2.21 461 147 162 15 511.51 73.40 462 147 191 44 242.15 3.35 463 147 192 45 242.09 3.32 464 148 151 3 838.04 3.57 465 148 152 4 836.32 4.25 466 148 191 43 242.19 3.33 467 149 152 3 856.23 3.73 468 149 153 4 841.75 3.48 469 150 153 3 870.13 10.30 470 150 154 4 836.05 0.63 471 150 162 12 511.49 73.39 472 151 154 3 847.96 0.38 473 151 157 6 669.04 10.47 474 151 191 40 242.16 3.31 475 154 157 3 887.06 0.17 476 154 160 6 649.85 4.69 477 156 160 4 857.02 2.84 478 157 160 3 853.59 1.12 479 157 162 5 864.69 0.30 480 157 192 35 241.25 3.32 481 157 196 39 197.75 1.48 482 157 200 43 196.99 1.53 483 157 204 47 196.21 1.74 484 161 205 44 196.19 1.73 485 161 207 46 195.97 1.64 486 162 188 26 242.00 3.36 487 162 205 43 196.25 1.72 488 162 206 44 196.09 1.70 489 162 207 45 196.09 1.70 490 163 207 44 195.97 1.64 491 163 230 67 121.95 0.68 492 164 184 20 242.30 3.29 493 164 185 21 242.15 3.33 494 164 188 24 242.12 3.34 495 164 207 43 196.16 1.71 496 164 208 44 195.90 1.60 497 164 209 45 195.63 1.49 498 165 209 44 194.53 0.96 499 166 169 3 877.36 0.10 500 166 170 4 855.80 2.34 501 166 176 10 244.73 2.89 502 166 208 42 196.00 1.59 503 166 209 43 195.88 1.60 504 166 210 44 194.31 0.83 505 167 170 3 855.81 2.34 506 167 172 5 838.80 2.60 507 168 172 4 842.68 2.07 508 168 174 6 379.00 36.13 509 168 175 7 378.99 36.13 510 168 176 8 252.16 0.15 511 168 180 12 252.13 0.15 512 169 172 3 863.53 1.67 513 169 174 5 263.73 2.10 514 169 175 6 252.16 0.16 515 169 176 7 252.15 0.16 516 175 180 5 853.82 1.52 517 176 180 4 851.03 2.90 518 176 208 32 197.52 1.36 519 177 180 3 865.99 3.70 520 177 181 4 849.39 3.72 521 178 181 3 857.72 0.44 522 178 182 4 849.39 3.72 523 178 220 42 125.45 0.07 524 178 223 45 125.31 0.01 525 179 182 3 850.25 3.32 526 179 183 4 850.26 3.31 527 180 183 3 850.27 3.31 528 180 184 4 850.23 3.29 529 181 184 3 864.61 3.85 530 181 185 4 841.10 1.28 531 181 208 27 197.02 1.62 532 181 220 39 125.45 0.07 533 182 185 3 845.22 3.02 534 182 186 4 841.08 1.27 535 182 223 41 124.45 0.18 536 182 224 42 124.42 0.17 537 182 225 43 124.38 0.19 538 182 226 44 124.34 0.21 539 183 186 3 851.00 4.12 540 183 187 4 846.57 1.90 541 184 187 3 865.49 3.40 542 184 188 4 840.68 1.04 543 185 188 3 861.33 5.49 544 185 189 4 840.68 1.04 545 185 206 21 197.67 1.36 546 185 207 22 197.67 1.36 547 185 208 23 197.59 1.39 548 185 225 40 124.23 0.24 549 185 226 41 124.14 0.24 550 186 189 3 861.33 5.49 551 186 190 4 840.51 0.97 552 186 225 39 124.26 0.22 553 187 190 3 839.59 1.44 554 187 191 4 839.59 1.44 555 188 191 3 840.95 1.19 556 188 192 4 839.79 1.53 557 188 206 18 197.59 1.41 558 189 192 3 863.53 13.40 559 189 193 4 838.86 1.06 560 189 201 12 197.88 1.38 561 189 204 15 197.74 1.39 562 189 206 17 197.68 1.36 563 189 225 36 124.13 0.25 564 189 227 38 124.09 0.23 565 190 193 3 842.50 2.90 566 190 194 4 779.62 28.54 567 191 194 3 854.82 3.44 568 191 195 4 778.40 28.14 569 191 196 5 770.65 32.02 570 192 195 3 852.34 4.67 571 192 196 4 675.49 12.30 572 192 197 5 673.36 13.37 573 192 201 9 197.91 1.37 574 192 204 12 197.77 1.36 575 192 206 14 197.68 1.36 576 193 197 4 452.19 79.94 577 193 198 5 303.04 50.12 578 193 201 8 197.94 1.38 579 196 200 4 837.32 0.78 580 197 200 3 862.89 12.01 581 197 201 4 837.34 0.77 582 198 201 3 857.87 3.70 583 198 202 4 837.34 0.76 584 199 202 3 857.87 3.70 585 199 203 4 837.34 0.76 586 200 203 3 844.70 1.93 587 200 204 4 772.50 33.16 588 205 227 22 123.62 0.35 589 206 225 19 124.19 0.27 590 206 226 20 123.84 0.34 591 206 227 21 123.83 0.35 592 207 227 20 123.84 0.35 593 207 228 21 122.55 0.55 594 207 229 22 122.55 0.54 595 207 230 23 122.55 0.54 596 208 212 4 875.37 1.27 597 208 220 12 124.38 0.45 598 208 226 18 124.20 0.28 599 208 228 20 123.84 0.35 600 208 229 21 123.00 0.39 601 208 230 22 122.99 0.39 602 209 230 21 122.97 0.39 603 211 220 9 237.02 11.63 604 212 217 5 274.56 7.13 605 212 220 8 204.81 3.68 606 212 229 17 124.24 0.27 607 212 231 19 123.98 0.32 608 212 234 22 122.00 0.90 609 212 243 31 55.30 0.20 610 213 216 3 873.27 5.71 611 213 217 4 753.97 40.98 612 213 234 21 121.93 0.93 613 213 239 26 65.28 1.20 614 213 243 30 55.33 0.21 615 214 217 3 868.25 7.25 616 214 239 25 61.63 0.61 617 214 242 28 56.04 0.14 618 214 243 29 55.73 0.01 619 215 219 4 880.74 4.28 620 216 219 3 865.77 3.21 621 216 220 4 847.32 2.81 622 217 220 3 856.64 0.80 623 217 221 4 844.88 4.04 624 217 229 12 124.45 0.20 625 217 242 25 55.77 0.01 626 217 243 26 50.66 0.10 627 217 246 29 42.05 0.09 628 218 221 3 852.00 3.14 629 218 222 4 844.90 4.03 630 218 245 27 46.13 0.44 631 218 246 28 41.97 0.14 632 218 248 30 41.63 0.30 633 219 222 3 852.11 0.43 634 219 223 4 844.91 4.03 635 220 223 3 854.64 5.58 636 220 226 6 260.67 2.29 637 220 229 9 124.47 0.21 638 221 226 5 211.63 2.43 639 221 229 8 124.47 0.21 640 221 246 25 41.59 0.29 641 221 247 26 39.32 0.71 642 223 226 3 848.36 1.80 643 223 247 24 41.45 0.31 644 224 227 3 917.77 2.23 645 226 229 3 1006.45 3.34 646 226 247 21 38.91 0.96 647 227 247 20 31.59 0.24 648 229 243 14 48.66 1.09 649 229 246 17 41.69 0.27 650 231 234 3 885.40 1.28 651 231 235 4 693.74 4.94 652 231 240 9 94.32 15.75 653 231 243 12 51.94 0.29 654 232 235 3 870.95 2.56 655 233 236 3 875.10 0.48 656 234 237 3 851.84 0.03 657 234 239 5 243.38 20.12 658 234 240 6 151.80 44.45 659 234 243 9 126.86 0.14 660 235 240 5 341.31 50.30 661 237 240 3 860.15 2.37 662 238 241 3 866.35 2.15 663 239 242 3 849.36 4.74 664 239 243 4 792.65 24.23 665 240 243 3 840.23 5.45 666 240 244 4 738.95 2.62 667 241 244 3 836.60 3.14 668 241 245 4 768.58 30.88 669 242 245 3 841.20 0.34 670 242 246 4 756.31 41.88 671 243 246 3 718.00 6.77 672 245 248 3 850.62 4.05