BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 141.88 14.82 2 3 204 201 141.59 14.69 3 3 205 202 140.33 14.07 4 3 224 221 113.63 0.68 5 3 225 222 113.59 0.69 6 3 227 224 113.50 0.66 7 4 34 30 738.78 33.87 8 4 35 31 738.77 33.87 9 4 36 32 738.75 33.88 10 4 37 33 738.73 33.87 11 4 38 34 738.72 33.87 12 4 205 201 140.81 14.29 13 5 37 32 738.75 33.87 14 5 38 33 738.75 33.87 15 5 221 216 113.59 0.69 16 5 227 222 113.59 0.69 17 5 247 242 18.14 0.23 18 6 37 31 738.77 33.87 19 6 38 32 738.75 33.87 20 6 40 34 738.73 33.87 21 6 90 84 152.21 18.43 22 6 124 118 143.13 15.16 23 6 161 155 143.11 15.17 24 6 205 199 142.92 15.12 25 6 207 201 142.08 14.93 26 6 227 221 113.64 0.68 27 6 228 222 113.59 0.70 28 6 229 223 113.58 0.70 29 7 37 30 738.81 33.87 30 7 38 31 738.77 33.87 31 7 39 32 738.74 33.87 32 7 40 33 738.74 33.87 33 7 229 222 113.59 0.70 34 7 240 233 51.31 0.11 35 7 243 236 42.88 0.25 36 7 244 237 36.09 0.78 37 7 247 240 21.77 0.04 38 8 40 32 738.75 33.87 39 8 42 34 738.73 33.88 40 8 229 221 113.83 0.67 41 8 230 222 113.59 0.70 42 8 231 223 113.57 0.70 43 9 40 31 738.77 33.87 44 9 41 32 738.73 33.88 45 9 42 33 738.73 33.88 46 9 231 222 113.59 0.70 47 9 235 226 79.41 0.00 48 9 240 231 51.31 0.12 49 10 42 32 738.74 33.88 50 10 64 54 455.04 0.89 51 10 92 82 154.53 19.50 52 10 95 85 153.24 19.83 53 10 230 220 115.29 1.26 54 10 231 221 113.77 0.65 55 10 232 222 113.58 0.70 56 10 235 225 84.45 0.03 57 11 14 3 867.71 3.89 58 11 20 9 739.88 33.99 59 11 23 12 739.78 34.04 60 11 24 13 739.77 34.05 61 11 27 16 739.75 34.05 62 11 41 30 738.81 33.87 63 11 42 31 738.77 33.87 64 11 43 32 738.74 33.88 65 11 51 40 619.79 28.53 66 11 64 53 455.24 0.96 67 11 235 224 106.37 0.46 68 11 236 225 79.73 0.06 69 12 44 32 738.76 33.87 70 12 64 52 455.17 0.94 71 12 95 83 154.57 19.51 72 12 97 85 154.50 19.55 73 12 232 220 115.30 1.26 74 13 44 31 738.77 33.87 75 14 44 30 738.79 33.87 76 14 236 222 113.47 0.72 77 15 19 4 835.31 1.49 78 15 20 5 820.50 2.98 79 15 47 32 737.96 34.02 80 16 19 3 860.30 5.35 81 16 20 4 835.31 1.49 82 16 47 31 737.96 34.02 83 17 20 3 854.15 1.32 84 17 21 4 840.57 0.64 85 17 47 30 737.40 34.12 86 17 50 33 676.35 31.04 87 18 21 3 844.70 2.45 88 18 22 4 840.57 0.64 89 19 22 3 857.44 0.42 90 19 23 4 839.24 3.18 91 20 23 3 845.29 3.52 92 20 24 4 841.92 1.84 93 20 47 27 737.32 34.11 94 20 50 30 669.33 27.57 95 20 51 31 619.30 27.51 96 20 54 34 462.75 0.07 97 21 24 3 862.75 0.38 98 21 25 4 841.92 1.84 99 21 54 33 462.67 0.03 100 22 25 3 852.60 0.14 101 22 26 4 846.61 0.49 102 23 26 3 862.15 6.29 103 23 27 4 844.25 2.78 104 23 235 212 109.78 0.55 105 23 236 213 97.13 3.04 106 23 237 214 80.10 0.21 107 23 238 215 79.47 0.04 108 24 27 3 851.66 0.63 109 24 28 4 844.30 2.75 110 24 51 27 504.31 20.84 111 24 54 30 461.73 0.43 112 24 55 31 460.34 0.20 113 25 28 3 856.68 3.44 114 25 29 4 850.11 0.14 115 26 29 3 853.18 1.69 116 26 30 4 859.48 3.31 117 26 238 212 75.06 0.79 118 27 30 3 866.15 2.00 119 27 55 28 460.36 0.21 120 27 59 32 457.26 0.87 121 27 235 208 96.17 3.38 122 27 237 210 79.58 0.01 123 27 240 213 55.15 1.80 124 27 241 214 50.41 0.48 125 28 54 26 461.66 0.47 126 28 55 27 460.25 0.16 127 28 56 28 460.18 0.12 128 28 58 30 459.76 0.29 129 28 59 31 459.56 0.28 130 30 58 28 459.58 0.32 131 30 241 211 50.94 0.18 132 30 244 214 46.88 0.69 133 30 245 215 42.98 0.21 134 30 248 218 41.14 0.18 135 31 58 27 456.28 0.80 136 31 248 217 37.19 0.36 137 32 39 7 739.19 33.90 138 32 58 26 456.22 0.77 139 32 59 27 456.19 0.76 140 32 248 216 28.82 0.14 141 33 36 3 858.27 0.89 142 33 37 4 814.85 2.94 143 34 37 3 857.64 0.57 144 34 58 24 455.55 0.96 145 37 244 207 38.59 1.05 146 37 247 210 22.03 0.09 147 37 248 211 18.18 0.21 148 38 205 167 143.09 15.19 149 39 59 20 455.65 0.92 150 39 60 21 455.63 0.93 151 39 244 205 36.11 1.06 152 40 60 20 455.63 0.93 153 40 61 21 455.62 0.93 154 40 62 22 455.51 0.88 155 40 90 50 154.30 19.34 156 41 51 10 460.53 0.08 157 41 55 14 458.12 0.71 158 41 59 18 456.13 0.81 159 41 60 19 455.92 0.88 160 41 61 20 455.62 0.94 161 41 62 21 455.62 0.93 162 42 62 20 455.62 0.94 163 42 63 21 455.49 0.88 164 42 64 22 455.13 0.93 165 42 90 48 154.90 19.32 166 42 91 49 154.91 19.34 167 42 92 50 154.62 19.48 168 42 124 82 154.47 19.41 169 42 230 188 115.34 1.24 170 43 47 4 752.09 32.36 171 43 48 5 691.08 62.86 172 43 51 8 462.59 0.03 173 43 62 19 455.94 0.89 174 43 64 21 455.47 0.87 175 44 47 3 846.22 2.72 176 44 48 4 832.14 4.83 177 44 64 20 455.53 0.89 178 45 48 3 848.40 3.81 179 47 50 3 857.72 2.41 180 47 51 4 840.64 1.04 181 48 51 3 854.10 3.01 182 48 52 4 840.64 1.04 183 48 61 13 456.38 0.83 184 48 62 14 456.36 0.83 185 48 63 15 456.21 0.84 186 48 88 40 155.19 19.28 187 49 52 3 853.63 0.24 188 49 53 4 840.61 1.04 189 50 53 3 863.73 4.75 190 50 54 4 842.28 0.21 191 51 54 3 859.63 2.71 192 51 55 4 842.27 0.21 193 52 55 3 860.54 1.27 194 52 56 4 859.64 2.71 195 52 57 5 840.51 0.67 196 52 59 7 615.96 30.54 197 52 60 8 459.53 0.29 198 52 61 9 456.39 0.83 199 55 59 4 743.50 35.57 200 55 61 6 457.44 0.73 201 56 59 3 855.84 1.33 202 59 244 185 41.74 0.23 203 60 89 29 154.53 19.39 204 60 90 30 154.40 19.43 205 61 89 28 154.81 19.34 206 61 90 29 154.20 19.39 207 62 88 26 154.94 19.36 208 62 90 28 154.27 19.36 209 63 78 15 157.92 19.11 210 63 83 20 156.79 19.37 211 63 88 25 155.50 19.14 212 63 90 27 155.00 19.30 213 63 91 28 154.85 19.37 214 63 92 29 154.77 19.41 215 64 92 28 154.85 19.37 216 65 92 27 155.25 19.44 217 65 98 33 155.31 19.53 218 65 112 47 156.68 19.41 219 66 78 12 158.13 19.07 220 66 79 13 157.91 19.12 221 66 80 14 157.68 19.02 222 66 91 25 155.93 19.23 223 66 93 27 155.62 19.39 224 66 112 46 156.68 19.41 225 66 116 50 157.34 19.12 226 67 77 10 158.33 19.10 227 67 78 11 158.25 19.09 228 67 79 12 158.09 19.09 229 67 112 45 157.18 19.20 230 68 108 40 157.16 19.21 231 68 111 43 157.21 19.18 232 68 112 44 157.18 19.20 233 69 74 5 498.30 21.31 234 69 79 10 159.43 18.86 235 70 74 4 870.42 1.35 236 70 79 9 159.56 18.80 237 70 81 11 159.65 18.77 238 71 79 8 159.69 18.75 239 71 81 10 159.69 18.76 240 71 82 11 159.70 18.76 241 74 77 3 871.60 1.82 242 78 82 4 851.26 3.60 243 79 82 3 856.34 1.07 244 79 83 4 826.26 5.51 245 80 83 3 840.33 1.88 246 80 84 4 826.31 5.54 247 80 115 35 286.18 24.12 248 80 116 36 285.94 24.23 249 80 119 39 370.99 6.04 250 80 121 41 370.94 6.04 251 81 84 3 847.70 4.58 252 81 85 4 826.32 5.55 253 81 119 38 373.65 6.46 254 82 85 3 856.23 1.10 255 82 86 4 826.26 5.58 256 83 86 3 847.12 2.41 257 83 87 4 826.25 5.58 258 83 88 5 697.13 57.65 259 83 121 38 434.26 14.71 260 84 87 3 832.28 2.56 261 84 119 35 443.78 19.48 262 84 120 36 468.87 6.94 263 84 121 37 434.26 14.71 264 88 121 33 537.66 0.91 265 89 121 32 537.66 0.91 266 89 122 33 537.64 0.92 267 90 122 32 537.65 0.92 268 90 124 34 537.61 0.91 269 90 161 71 537.64 0.92 270 91 116 25 537.83 0.89 271 91 121 30 537.81 0.90 272 91 122 31 537.77 0.89 273 91 123 32 537.64 0.92 274 91 124 33 537.64 0.92 275 92 95 3 902.90 2.71 276 92 124 32 537.65 0.92 277 92 126 34 537.62 0.92 278 92 230 138 163.05 22.43 279 93 109 16 537.77 0.88 280 93 112 19 537.92 0.90 281 93 113 20 537.83 0.89 282 93 116 23 537.97 0.90 283 93 123 30 537.83 0.89 284 93 124 31 537.77 0.88 285 93 125 32 537.68 0.90 286 93 126 33 537.62 0.92 287 94 99 5 818.00 1.01 288 94 109 15 537.77 0.88 289 94 126 32 537.69 0.90 290 95 98 3 852.64 0.06 291 95 99 4 842.22 4.13 292 95 126 31 537.69 0.90 293 95 165 70 537.67 0.89 294 95 230 135 163.42 22.37 295 96 99 3 842.22 4.13 296 96 100 4 842.21 4.14 297 96 165 69 537.69 0.90 298 96 167 71 536.66 0.79 299 96 170 74 495.54 0.64 300 97 100 3 850.13 0.18 301 97 101 4 827.01 3.46 302 97 102 5 827.01 3.46 303 98 102 4 768.28 23.86 304 98 103 5 768.27 23.86 305 98 104 6 539.86 0.02 306 99 103 4 739.87 36.12 307 99 104 5 538.38 0.76 308 99 106 7 538.38 0.77 309 99 109 10 538.38 0.76 310 99 126 27 537.93 0.87 311 99 127 28 537.89 0.88 312 99 146 47 538.00 0.83 313 104 108 4 829.33 0.18 314 104 112 8 751.51 22.96 315 105 108 3 835.75 0.90 316 105 109 4 833.64 1.95 317 106 109 3 839.00 2.52 318 106 110 4 829.38 0.20 319 106 145 39 538.69 0.77 320 106 146 40 538.03 0.84 321 106 149 43 538.69 0.76 322 107 110 3 843.39 6.81 323 107 111 4 829.38 0.20 324 108 111 3 861.12 2.04 325 108 112 4 843.39 6.81 326 109 112 3 850.69 3.17 327 109 113 4 843.38 6.80 328 109 125 16 538.42 0.70 329 109 150 41 538.97 0.70 330 110 113 3 858.89 0.95 331 110 114 4 843.38 6.80 332 110 149 39 538.95 0.70 333 110 150 40 538.95 0.70 334 110 152 42 539.45 0.67 335 110 153 43 539.45 0.67 336 111 114 3 858.03 0.52 337 111 115 4 843.30 6.84 338 112 115 3 858.31 5.48 339 112 116 4 833.73 2.06 340 113 116 3 866.33 5.53 341 113 117 4 846.33 4.48 342 113 123 10 539.07 0.76 343 113 125 12 538.93 0.70 344 113 150 37 539.09 0.73 345 113 153 40 546.98 2.83 346 113 160 47 539.38 0.64 347 114 117 3 850.85 2.22 348 114 118 4 846.35 4.45 349 114 153 39 550.40 1.11 350 115 118 3 852.68 1.29 351 115 119 4 846.36 4.45 352 116 119 3 852.68 1.29 353 116 120 4 846.35 4.45 354 116 121 5 635.23 23.78 355 117 120 3 836.14 0.66 356 117 121 4 774.65 31.40 357 117 153 36 638.91 22.02 358 117 154 37 640.62 21.18 359 117 155 38 655.02 28.38 360 117 160 43 591.24 1.76 361 122 159 37 725.66 33.24 362 123 159 36 725.75 33.23 363 123 160 37 725.71 33.23 364 123 161 38 725.70 33.23 365 124 161 37 725.70 33.23 366 124 163 39 725.68 33.23 367 124 207 83 298.38 0.46 368 124 230 106 162.97 22.47 369 125 150 25 725.99 33.20 370 125 160 35 725.79 33.23 371 125 161 36 725.75 33.23 372 125 162 37 725.70 33.23 373 125 163 38 725.70 33.23 374 126 163 37 725.70 33.23 375 126 165 39 725.67 33.23 376 126 230 104 163.15 22.39 377 127 143 16 726.23 33.23 378 127 146 19 726.17 33.21 379 127 147 20 726.16 33.21 380 127 162 35 725.81 33.23 381 127 163 36 725.75 33.23 382 127 164 37 725.73 33.24 383 127 165 38 725.72 33.24 384 127 188 61 313.53 1.05 385 128 143 15 726.24 33.23 386 128 146 18 726.23 33.23 387 128 164 36 725.75 33.23 388 128 165 37 725.73 33.24 389 128 167 39 725.62 33.20 390 129 133 4 840.85 0.71 391 129 134 5 754.39 24.71 392 129 139 10 726.29 33.22 393 129 142 13 726.31 33.23 394 129 164 35 725.81 33.23 395 129 166 37 725.73 33.24 396 129 167 38 725.71 33.23 397 129 168 39 725.63 33.22 398 130 133 3 850.53 4.12 399 130 134 4 842.35 0.01 400 130 167 37 725.72 33.23 401 130 168 38 725.69 33.23 402 131 134 3 852.07 1.40 403 131 135 4 842.36 0.02 404 131 168 37 725.71 33.23 405 131 172 41 686.33 25.96 406 131 174 43 641.90 30.40 407 132 135 3 856.73 0.93 408 132 136 4 842.36 0.01 409 133 136 3 846.36 1.12 410 133 137 4 840.49 0.90 411 133 138 5 738.49 27.82 412 133 142 9 726.49 33.29 413 134 137 3 840.00 1.11 414 134 139 5 726.77 33.15 415 134 164 30 725.97 33.20 416 134 168 34 725.73 33.24 417 134 184 50 314.07 0.96 418 135 174 39 705.76 30.21 419 138 141 3 850.99 3.57 420 138 142 4 834.21 0.20 421 139 142 3 856.49 0.83 422 139 143 4 835.87 0.98 423 139 184 45 313.75 1.07 424 140 143 3 855.99 3.10 425 140 144 4 841.62 3.87 426 140 187 47 313.68 1.05 427 141 144 3 855.99 3.10 428 141 145 4 841.63 3.88 429 142 145 3 848.61 0.55 430 142 146 4 843.05 3.19 431 143 146 3 843.08 3.18 432 143 147 4 843.16 3.12 433 143 164 21 725.82 33.23 434 143 184 41 313.69 1.07 435 143 188 45 313.58 1.04 436 143 191 48 313.50 1.07 437 144 147 3 863.57 4.88 438 144 148 4 843.16 3.12 439 144 191 47 313.48 1.06 440 145 148 3 855.91 1.04 441 145 149 4 852.15 0.83 442 146 149 3 865.24 2.40 443 146 150 4 855.91 1.04 444 147 150 3 884.72 0.16 445 147 151 4 838.54 1.05 446 147 188 41 313.50 1.05 447 147 191 44 313.46 1.06 448 148 151 3 852.14 0.81 449 148 191 43 313.43 1.05 450 148 195 47 308.05 2.60 451 150 153 3 876.70 3.61 452 150 162 12 725.80 33.23 453 151 154 3 858.89 1.90 454 151 192 41 312.56 0.96 455 151 195 44 302.68 0.14 456 151 196 45 302.43 0.16 457 154 157 3 877.19 0.12 458 154 160 6 725.97 33.16 459 154 204 50 300.38 0.04 460 156 159 3 869.16 1.09 461 156 160 4 850.77 0.72 462 157 192 35 312.24 0.69 463 157 196 39 300.87 0.09 464 157 200 43 300.47 0.08 465 157 203 46 300.41 0.06 466 157 204 47 300.33 0.06 467 158 161 3 927.65 3.25 468 158 203 45 300.39 0.07 469 158 205 47 300.33 0.06 470 160 204 44 300.32 0.04 471 161 205 44 300.15 0.01 472 161 207 46 298.44 0.47 473 162 188 26 313.33 1.10 474 162 192 30 312.38 0.77 475 162 204 42 300.36 0.07 476 162 205 43 300.30 0.06 477 162 206 44 298.44 0.47 478 162 207 45 298.44 0.47 479 163 207 44 298.41 0.47 480 163 208 45 298.39 0.48 481 163 209 46 296.87 0.66 482 163 230 67 163.20 22.37 483 164 184 20 313.58 1.03 484 164 185 21 313.54 1.02 485 164 188 24 313.53 1.03 486 164 207 43 298.46 0.46 487 164 208 44 298.31 0.48 488 164 209 45 298.30 0.49 489 165 209 44 297.31 0.42 490 166 169 3 865.23 0.49 491 166 170 4 850.14 5.74 492 166 176 10 314.56 0.90 493 166 208 42 298.47 0.48 494 166 209 43 298.37 0.47 495 166 210 44 297.25 0.43 496 167 170 3 852.51 4.55 497 168 172 4 833.69 2.50 498 168 174 6 572.84 3.97 499 168 176 8 315.48 0.49 500 169 172 3 861.80 0.94 501 169 174 5 410.13 32.90 502 169 175 6 316.51 1.01 503 169 176 7 315.48 0.49 504 176 180 4 835.06 0.22 505 176 208 32 299.31 0.12 506 177 180 3 843.39 3.05 507 177 181 4 839.51 2.00 508 178 181 3 839.52 2.00 509 178 182 4 842.71 0.40 510 178 219 41 221.46 1.31 511 178 220 42 170.09 24.37 512 178 223 45 168.68 23.86 513 179 182 3 842.73 0.39 514 179 183 4 844.84 0.67 515 180 183 3 855.42 1.02 516 180 184 4 847.65 2.86 517 181 184 3 855.42 1.02 518 181 185 4 832.68 1.00 519 181 208 27 300.18 0.01 520 181 220 39 170.08 24.37 521 181 226 45 167.23 23.77 522 182 185 3 855.42 1.02 523 182 186 4 834.70 0.10 524 182 223 41 167.54 23.89 525 182 224 42 167.52 23.89 526 182 225 43 167.38 23.84 527 182 226 44 167.06 23.86 528 183 186 3 847.57 0.40 529 183 187 4 839.84 4.16 530 184 187 3 846.22 7.04 531 184 188 4 839.83 4.17 532 185 188 3 844.57 1.79 533 185 189 4 833.22 0.89 534 185 206 21 300.37 0.07 535 185 207 22 300.37 0.07 536 185 208 23 300.36 0.07 537 185 225 40 166.90 23.84 538 185 226 41 166.77 23.82 539 186 189 3 847.08 0.55 540 186 190 4 840.50 2.74 541 186 225 39 167.01 23.88 542 187 190 3 841.69 2.15 543 187 191 4 839.82 4.19 544 188 191 3 847.12 0.57 545 188 192 4 842.89 5.27 546 188 206 18 300.37 0.07 547 189 192 3 853.38 0.03 548 189 193 4 840.27 3.96 549 189 201 12 300.70 0.16 550 189 204 15 300.63 0.12 551 189 205 16 300.61 0.12 552 189 206 17 300.37 0.07 553 189 225 36 166.85 23.86 554 189 227 38 166.78 23.82 555 190 193 3 857.00 1.51 556 190 194 4 833.24 0.44 557 191 194 3 839.94 2.59 558 191 195 4 824.42 3.97 559 192 195 3 849.11 7.32 560 192 196 4 823.80 3.71 561 192 197 5 814.69 8.26 562 192 201 9 301.25 0.43 563 192 204 12 300.63 0.10 564 193 196 3 838.16 3.48 565 193 197 4 690.77 70.22 566 193 198 5 480.68 41.40 567 193 201 8 301.33 0.47 568 196 200 4 830.50 0.03 569 197 200 3 852.02 2.69 570 197 201 4 830.93 0.24 571 198 201 3 851.78 3.48 572 198 202 4 830.94 0.24 573 199 202 3 861.69 1.47 574 199 203 4 833.10 1.31 575 200 203 3 850.45 7.81 576 200 204 4 833.07 1.32 577 201 204 3 852.72 0.39 578 205 227 22 164.62 22.95 579 206 225 19 166.69 23.82 580 206 226 20 165.67 23.37 581 206 227 21 165.55 23.32 582 207 227 20 165.56 23.31 583 207 228 21 163.62 22.45 584 207 229 22 163.48 22.39 585 207 230 23 163.44 22.39 586 208 212 4 856.33 4.71 587 208 220 12 167.74 24.28 588 208 226 18 166.65 23.80 589 208 228 20 164.69 22.89 590 208 229 21 163.49 22.39 591 208 230 22 163.49 22.39 592 209 230 21 163.47 22.39 593 211 216 5 838.68 1.81 594 212 216 4 830.60 2.23 595 212 217 5 696.25 64.94 596 212 220 8 342.68 7.84 597 212 229 17 165.50 23.25 598 212 231 19 163.61 22.36 599 212 234 22 163.43 22.39 600 212 243 31 98.13 23.30 601 213 216 3 861.05 6.38 602 213 217 4 830.60 2.23 603 213 234 21 163.47 22.41 604 213 243 30 98.13 23.30 605 214 217 3 865.36 7.77 606 214 239 25 115.56 32.00 607 214 242 28 98.21 23.33 608 214 243 29 98.20 23.32 609 216 219 3 875.12 2.89 610 216 220 4 832.25 1.34 611 217 220 3 847.68 1.17 612 217 221 4 836.21 3.32 613 217 229 12 166.81 23.68 614 217 243 26 97.56 23.57 615 217 246 29 87.09 27.79 616 218 221 3 861.92 5.96 617 218 222 4 843.98 0.57 618 218 246 28 86.26 28.16 619 219 222 3 848.21 1.54 620 219 223 4 847.55 1.22 621 220 223 3 859.88 4.95 622 220 226 6 223.69 2.17 623 220 229 9 167.46 23.84 624 221 226 5 378.64 81.14 625 221 227 6 169.22 23.09 626 221 228 7 167.45 23.84 627 221 229 8 168.99 23.07 628 221 246 25 84.76 27.41 629 221 247 26 26.42 0.32 630 223 226 3 834.78 4.59 631 229 243 14 92.28 24.95 632 231 234 3 890.62 6.14 633 231 235 4 707.13 60.64 634 231 240 9 98.12 23.29 635 231 243 12 97.34 23.63 636 232 235 3 853.52 0.50 637 233 236 3 844.83 3.85 638 234 237 3 860.25 2.10 639 234 240 6 236.12 44.78 640 234 243 9 98.14 23.29 641 235 240 5 236.13 44.78 642 237 240 3 868.83 1.95 643 238 241 3 881.45 0.01 644 239 242 3 862.80 4.96 645 239 243 4 840.13 1.97 646 240 243 3 839.64 1.73 647 240 244 4 838.25 1.02 648 241 244 3 838.25 1.02 649 241 245 4 838.25 1.02 650 242 245 3 838.25 1.02 651 242 246 4 838.25 1.02 652 243 246 3 851.55 0.46 653 244 247 3 859.16 4.49 654 244 248 4 843.16 3.52 655 245 248 3 847.88 2.23