BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 140.09 14.52 2 3 204 201 139.83 14.65 3 3 205 202 139.33 14.43 4 3 224 221 112.67 1.08 5 3 227 224 112.45 1.02 6 4 34 30 812.36 1.17 7 4 35 31 812.35 1.17 8 4 36 32 812.27 1.16 9 4 37 33 812.27 1.17 10 4 38 34 812.27 1.17 11 4 205 201 139.83 14.65 12 5 37 32 812.30 1.17 13 5 38 33 812.30 1.17 14 5 221 216 112.67 1.08 15 5 227 222 112.67 1.08 16 5 247 242 18.79 0.26 17 6 37 31 812.32 1.17 18 6 38 32 812.30 1.17 19 6 40 34 812.30 1.17 20 6 90 84 351.40 0.11 21 6 124 118 307.28 6.03 22 6 161 155 287.74 15.46 23 6 205 199 143.62 15.53 24 6 207 201 139.73 14.61 25 6 227 221 112.73 1.08 26 6 228 222 112.69 1.08 27 6 229 223 112.69 1.08 28 7 37 30 812.36 1.17 29 7 38 31 812.33 1.17 30 7 39 32 812.30 1.17 31 7 40 33 812.30 1.17 32 7 229 222 112.69 1.08 33 7 240 233 49.62 0.18 34 7 243 236 42.16 0.18 35 7 244 237 33.42 0.75 36 7 247 240 20.82 0.88 37 8 40 32 812.30 1.17 38 8 42 34 812.28 1.17 39 8 229 221 112.97 0.98 40 8 230 222 112.67 1.08 41 8 231 223 112.67 1.08 42 9 40 31 812.33 1.17 43 9 41 32 812.30 1.18 44 9 42 33 812.28 1.17 45 9 231 222 112.69 1.08 46 9 235 226 82.01 0.30 47 9 240 231 52.85 1.43 48 10 42 32 812.30 1.17 49 10 64 54 452.47 0.35 50 10 92 82 351.91 0.09 51 10 95 85 351.64 0.01 52 10 230 220 114.19 0.69 53 10 231 221 112.98 0.96 54 10 232 222 112.68 1.08 55 10 235 225 87.78 0.06 56 11 14 3 879.63 1.03 57 11 20 9 812.96 1.18 58 11 23 12 812.95 1.18 59 11 24 13 812.94 1.19 60 11 27 16 812.91 1.19 61 11 41 30 812.36 1.17 62 11 42 31 812.33 1.16 63 11 43 32 812.31 1.17 64 11 51 40 623.94 38.02 65 11 64 53 452.71 0.41 66 11 235 224 106.62 1.54 67 11 236 225 82.61 0.04 68 12 44 32 812.32 1.17 69 12 64 52 452.67 0.39 70 12 95 83 351.91 0.06 71 12 97 85 352.13 0.04 72 12 232 220 114.19 0.69 73 13 44 31 812.34 1.16 74 14 44 30 812.36 1.17 75 14 236 222 112.37 0.99 76 15 19 4 836.03 1.01 77 15 20 5 834.83 0.39 78 15 47 32 812.07 1.29 79 16 19 3 858.69 1.33 80 16 20 4 851.04 5.08 81 16 47 31 812.08 1.29 82 17 20 3 866.03 2.42 83 17 21 4 855.83 2.68 84 17 50 33 759.51 6.15 85 18 21 3 861.31 0.06 86 18 22 4 855.82 2.68 87 19 22 3 866.38 2.60 88 19 23 4 842.22 2.81 89 20 23 3 853.44 2.81 90 20 24 4 851.59 0.01 91 20 47 27 811.95 1.27 92 20 50 30 751.09 6.52 93 20 51 31 644.62 27.69 94 21 24 3 860.39 0.41 95 21 25 4 844.08 3.77 96 21 54 33 500.59 22.38 97 22 25 3 865.67 4.11 98 22 26 4 848.04 5.75 99 23 26 3 862.65 2.64 100 23 27 4 856.82 1.36 101 23 235 212 110.48 1.83 102 23 236 213 90.14 2.17 103 23 237 214 84.62 1.21 104 23 238 215 79.02 0.91 105 24 27 3 856.84 1.35 106 24 28 4 852.80 0.65 107 24 51 27 621.00 37.71 108 24 54 30 456.10 0.17 109 24 55 31 455.61 0.26 110 25 28 3 864.75 3.88 111 25 29 4 858.22 2.06 112 26 29 3 873.00 5.48 113 26 30 4 865.88 3.31 114 26 238 212 72.84 0.15 115 27 30 3 870.07 4.00 116 27 55 28 455.61 0.25 117 27 59 32 454.61 0.14 118 27 235 208 91.15 1.66 119 27 237 210 82.00 0.31 120 27 238 211 72.88 0.18 121 27 240 213 58.43 0.91 122 27 241 214 49.38 0.04 123 28 54 26 455.94 0.10 124 28 55 27 455.52 0.21 125 28 56 28 455.50 0.21 126 28 58 30 455.28 0.17 127 28 59 31 454.98 0.04 128 30 58 28 454.48 0.13 129 30 241 211 49.34 0.03 130 30 244 214 48.22 0.42 131 30 245 215 46.43 0.48 132 31 248 217 34.34 1.17 133 32 37 5 831.70 3.04 134 32 39 7 813.47 1.21 135 32 58 26 453.15 0.45 136 32 59 27 453.15 0.45 137 32 248 216 30.10 0.46 138 33 36 3 852.01 6.38 139 33 37 4 831.72 3.04 140 34 37 3 855.00 4.89 141 34 58 24 452.74 0.35 142 37 244 207 37.32 0.01 143 37 247 210 22.45 0.47 144 37 248 211 17.27 0.48 145 38 205 167 143.68 15.54 146 39 59 20 452.79 0.39 147 39 60 21 452.79 0.39 148 39 244 205 33.40 0.76 149 40 60 20 452.79 0.39 150 40 61 21 452.78 0.39 151 40 62 22 452.76 0.39 152 40 90 50 351.87 0.07 153 41 51 10 455.82 0.07 154 41 55 14 455.00 0.01 155 41 59 18 453.14 0.45 156 41 60 19 453.09 0.47 157 41 61 20 452.78 0.39 158 41 62 21 452.78 0.39 159 42 62 20 452.78 0.39 160 42 63 21 452.75 0.39 161 42 64 22 452.68 0.42 162 42 90 48 352.05 0.10 163 42 91 49 351.97 0.07 164 42 92 50 351.94 0.07 165 42 124 82 351.75 0.09 166 43 47 4 819.12 1.05 167 43 48 5 725.46 6.25 168 43 51 8 620.71 37.80 169 43 62 19 453.07 0.48 170 43 64 21 452.76 0.40 171 44 47 3 836.62 0.16 172 44 48 4 826.75 4.78 173 44 64 20 452.81 0.40 174 45 48 3 858.14 2.20 175 47 50 3 852.88 0.45 176 47 51 4 839.03 1.96 177 48 51 3 862.19 5.10 178 48 52 4 839.07 1.95 179 48 61 13 453.48 0.41 180 48 62 14 453.45 0.40 181 48 63 15 453.29 0.40 182 48 88 40 352.60 0.03 183 49 52 3 852.98 0.42 184 49 53 4 852.89 0.47 185 50 53 3 860.11 0.70 186 50 54 4 852.91 0.45 187 51 54 3 858.30 2.23 188 51 55 4 846.39 2.79 189 52 55 3 859.34 1.72 190 52 56 4 862.74 4.46 191 52 57 5 846.38 2.79 192 52 59 7 551.85 1.19 193 52 60 8 454.83 0.08 194 52 61 9 454.67 0.12 195 55 59 4 764.77 33.57 196 55 61 6 504.59 24.96 197 56 59 3 881.39 1.02 198 60 89 29 351.94 0.07 199 60 90 30 351.90 0.08 200 61 89 28 352.03 0.08 201 61 90 29 351.91 0.08 202 62 88 26 352.14 0.12 203 62 90 28 351.93 0.07 204 63 78 15 354.07 0.12 205 63 83 20 353.05 0.01 206 63 88 25 352.67 0.01 207 63 90 27 352.07 0.10 208 63 91 28 352.00 0.10 209 63 92 29 352.03 0.10 210 64 92 28 352.02 0.10 211 65 92 27 352.31 0.04 212 65 98 33 352.35 0.02 213 65 112 47 352.89 0.04 214 66 78 12 354.49 0.03 215 66 79 13 354.14 0.14 216 66 80 14 354.05 0.10 217 66 91 25 352.75 0.06 218 66 93 27 352.58 0.03 219 66 112 46 352.88 0.03 220 66 116 50 352.98 0.07 221 67 77 10 354.73 0.13 222 67 78 11 354.66 0.10 223 67 79 12 354.35 0.04 224 67 112 45 352.97 0.07 225 68 108 40 352.97 0.07 226 68 111 43 352.99 0.07 227 68 112 44 352.97 0.07 228 69 74 5 559.17 3.00 229 69 79 10 355.16 0.12 230 70 74 4 880.82 1.29 231 70 79 9 355.16 0.12 232 70 81 11 355.18 0.12 233 71 79 8 355.28 0.11 234 71 81 10 355.28 0.10 235 71 82 11 355.27 0.10 236 74 77 3 868.99 4.66 237 78 82 4 839.66 0.17 238 79 82 3 859.38 7.32 239 79 83 4 841.19 0.26 240 80 83 3 851.82 3.50 241 80 84 4 841.20 0.26 242 80 115 35 357.23 0.50 243 80 116 36 355.99 0.12 244 80 119 39 358.93 0.30 245 80 121 41 358.89 0.33 246 81 84 3 851.78 3.52 247 81 85 4 841.21 0.25 248 81 119 38 360.17 0.56 249 82 85 3 856.55 0.15 250 82 86 4 841.20 0.25 251 83 86 3 850.14 3.35 252 83 87 4 831.60 0.42 253 83 88 5 608.17 33.10 254 83 121 38 392.31 3.03 255 84 87 3 830.08 0.34 256 84 119 35 458.95 0.35 257 84 121 37 391.56 3.41 258 88 91 3 995.00 0.91 259 88 121 33 536.00 1.47 260 89 121 32 536.00 1.47 261 89 122 33 536.00 1.48 262 90 122 32 536.01 1.47 263 90 124 34 535.99 1.48 264 90 161 71 536.00 1.48 265 91 116 25 536.24 1.44 266 91 121 30 536.23 1.44 267 91 122 31 536.17 1.44 268 91 123 32 536.00 1.47 269 91 124 33 536.00 1.48 270 92 95 3 901.08 0.14 271 92 124 32 536.01 1.47 272 92 126 34 535.97 1.48 273 92 230 138 115.57 0.01 274 93 109 16 536.32 1.43 275 93 112 19 536.45 1.47 276 93 113 20 536.42 1.47 277 93 116 23 536.46 1.47 278 93 123 30 536.23 1.44 279 93 124 31 536.21 1.43 280 93 125 32 535.97 1.48 281 93 126 33 535.97 1.48 282 94 99 5 679.23 0.32 283 94 104 10 536.41 1.46 284 94 109 15 536.40 1.45 285 94 126 32 535.98 1.48 286 95 98 3 860.12 1.90 287 95 99 4 833.18 2.86 288 95 126 31 535.98 1.49 289 95 165 70 535.81 1.45 290 95 230 135 116.92 0.45 291 96 99 3 851.41 6.25 292 96 100 4 830.62 3.63 293 96 165 69 535.78 1.46 294 96 167 71 533.26 1.49 295 97 100 3 849.93 1.52 296 97 101 4 832.68 2.60 297 98 102 4 547.67 2.96 298 98 103 5 538.40 1.68 299 98 104 6 537.39 1.65 300 99 103 4 537.42 1.63 301 99 104 5 537.11 1.51 302 99 106 7 537.14 1.52 303 99 109 10 537.11 1.50 304 99 126 27 536.42 1.45 305 99 127 28 536.38 1.44 306 99 146 47 536.45 1.45 307 100 103 3 849.88 3.18 308 104 108 4 786.51 29.74 309 104 112 8 702.03 5.94 310 105 108 3 853.80 0.14 311 105 109 4 845.20 2.07 312 106 109 3 851.91 1.08 313 106 110 4 845.22 2.08 314 106 145 39 536.57 1.47 315 106 146 40 536.49 1.48 316 106 149 43 536.83 1.44 317 107 110 3 849.56 0.09 318 107 111 4 845.21 2.08 319 108 111 3 853.44 5.99 320 108 112 4 843.56 2.90 321 109 112 3 860.36 5.49 322 109 113 4 843.56 2.90 323 109 125 16 536.66 1.54 324 109 150 41 537.19 1.32 325 110 113 3 850.90 4.00 326 110 114 4 841.85 1.96 327 110 149 39 537.14 1.33 328 110 150 40 537.13 1.33 329 110 152 42 549.62 7.13 330 110 153 43 549.63 7.12 331 111 114 3 850.91 4.00 332 111 115 4 839.77 0.92 333 112 115 3 859.50 0.29 334 112 116 4 844.73 1.75 335 113 116 3 857.49 0.71 336 113 117 4 854.69 2.62 337 113 123 10 537.60 1.42 338 113 125 12 537.26 1.27 339 113 150 37 542.12 3.39 340 113 153 40 549.67 7.12 341 113 154 41 550.37 6.78 342 113 160 47 541.96 3.52 343 114 117 3 859.19 0.40 344 114 118 4 854.68 2.64 345 114 153 39 549.67 7.12 346 115 118 3 855.61 2.19 347 115 119 4 854.70 2.65 348 116 119 3 861.67 0.84 349 116 120 4 850.54 4.70 350 116 121 5 688.75 7.88 351 117 120 3 848.83 0.32 352 117 121 4 707.43 3.42 353 117 153 36 588.34 0.86 354 117 154 37 589.81 1.59 355 117 155 38 597.62 2.31 356 117 160 43 588.12 0.92 357 122 159 37 666.95 1.21 358 123 159 36 667.00 1.19 359 123 160 37 666.96 1.21 360 123 161 38 666.97 1.22 361 124 161 37 666.99 1.22 362 124 163 39 666.97 1.21 363 124 230 106 115.37 0.12 364 125 150 25 667.28 1.20 365 125 160 35 667.07 1.21 366 125 161 36 667.04 1.21 367 125 162 37 667.00 1.21 368 125 163 38 667.00 1.21 369 126 163 37 667.01 1.21 370 126 165 39 666.99 1.20 371 126 230 104 116.69 0.55 372 127 143 16 667.75 1.14 373 127 146 19 667.66 1.15 374 127 147 20 667.61 1.11 375 127 150 23 667.54 1.15 376 127 162 35 667.08 1.21 377 127 163 36 667.03 1.21 378 127 164 37 667.01 1.21 379 127 165 38 667.01 1.21 380 127 188 61 222.12 1.28 381 128 143 15 667.79 1.15 382 128 146 18 667.81 1.19 383 128 164 36 667.02 1.21 384 128 165 37 667.01 1.21 385 128 167 39 666.79 1.13 386 129 133 4 828.72 2.18 387 129 134 5 770.10 26.21 388 129 139 10 667.88 1.19 389 129 142 13 667.87 1.18 390 129 143 14 667.88 1.19 391 129 164 35 667.08 1.21 392 129 166 37 667.02 1.21 393 129 167 38 667.00 1.21 394 129 168 39 666.94 1.20 395 130 133 3 848.90 3.42 396 130 134 4 837.56 2.29 397 130 167 37 667.00 1.21 398 130 168 38 666.97 1.21 399 131 134 3 854.50 0.61 400 131 135 4 837.56 2.29 401 131 168 37 666.98 1.22 402 131 172 41 621.25 0.85 403 131 174 43 561.77 0.20 404 132 135 3 846.24 4.38 405 132 136 4 843.99 5.50 406 133 136 3 861.18 3.09 407 133 137 4 837.53 2.31 408 133 138 5 715.22 3.83 409 133 139 6 696.63 13.12 410 133 142 9 668.07 1.14 411 134 137 3 838.88 8.06 412 134 139 5 696.63 13.12 413 134 168 34 667.01 1.20 414 134 180 46 228.35 2.77 415 138 141 3 851.68 5.36 416 138 142 4 845.23 5.24 417 139 142 3 856.49 0.39 418 139 143 4 848.00 3.86 419 139 184 45 222.70 1.33 420 140 143 3 856.42 0.35 421 140 144 4 843.00 2.30 422 140 187 47 222.53 1.41 423 141 144 3 863.46 3.87 424 141 145 4 846.72 1.74 425 142 145 3 853.58 1.77 426 142 146 4 846.72 1.74 427 143 146 3 853.52 1.75 428 143 147 4 846.68 1.72 429 143 164 21 667.10 1.20 430 143 184 41 222.58 1.39 431 143 188 45 222.16 1.29 432 144 147 3 860.13 5.04 433 144 148 4 845.13 2.48 434 144 191 47 222.07 1.28 435 145 148 3 846.68 1.73 436 145 149 4 845.14 2.49 437 146 149 3 853.46 0.94 438 146 150 4 858.44 1.55 439 147 150 3 877.30 1.61 440 147 151 4 835.21 1.48 441 147 188 41 222.16 1.28 442 147 191 44 222.06 1.29 443 148 151 3 857.01 3.92 444 148 195 47 221.68 1.16 445 150 153 3 859.78 1.95 446 150 154 4 856.64 0.38 447 150 162 12 667.12 1.18 448 151 154 3 859.78 1.95 449 151 192 41 221.74 1.15 450 151 195 44 221.27 0.97 451 151 196 45 220.31 1.42 452 154 157 3 892.43 3.20 453 154 160 6 667.20 1.16 454 154 204 50 203.77 0.13 455 156 159 3 861.58 6.26 456 157 192 35 221.72 1.17 457 157 196 39 218.69 0.76 458 157 200 43 203.99 0.22 459 157 203 46 203.78 0.13 460 157 204 47 203.69 0.16 461 158 203 45 203.78 0.13 462 158 205 47 203.67 0.18 463 160 204 44 203.60 0.19 464 161 205 44 202.37 0.15 465 161 207 46 199.15 0.77 466 162 188 26 221.81 1.18 467 162 192 30 221.72 1.16 468 162 204 42 203.71 0.16 469 162 205 43 202.96 0.07 470 162 206 44 194.18 1.75 471 162 207 45 194.16 1.75 472 163 207 44 194.09 1.72 473 163 208 45 194.06 1.72 474 163 209 46 193.72 1.66 475 163 230 67 116.70 0.57 476 164 184 20 222.21 1.29 477 164 185 21 222.09 1.28 478 164 188 24 222.06 1.28 479 164 207 43 194.22 1.74 480 164 208 44 194.13 1.76 481 164 209 45 193.81 1.64 482 165 209 44 193.56 1.74 483 166 169 3 871.79 3.65 484 166 170 4 847.53 2.60 485 166 176 10 228.00 2.75 486 166 208 42 194.22 1.74 487 166 210 44 193.56 1.74 488 167 170 3 855.68 6.31 489 168 172 4 775.75 25.14 490 168 174 6 374.56 3.48 491 168 176 8 228.19 2.84 492 169 172 3 865.36 2.61 493 169 174 5 307.70 36.90 494 169 175 6 228.22 2.85 495 169 176 7 228.15 2.82 496 170 209 39 194.18 1.77 497 170 210 40 193.47 1.93 498 170 211 41 193.33 1.86 499 175 180 5 843.47 0.48 500 176 180 4 838.80 1.84 501 176 208 32 200.70 0.57 502 177 180 3 860.69 0.23 503 177 181 4 843.84 4.36 504 178 181 3 864.24 2.00 505 178 182 4 843.84 4.36 506 178 219 41 134.32 2.83 507 178 220 42 133.81 2.58 508 178 223 45 131.54 2.77 509 179 182 3 863.07 2.59 510 179 183 4 847.47 2.53 511 180 183 3 858.62 3.03 512 180 184 4 858.62 3.04 513 181 184 3 858.62 3.04 514 181 185 4 858.59 3.04 515 181 208 27 203.09 0.42 516 181 220 39 133.81 2.58 517 181 226 45 119.84 0.50 518 182 185 3 858.62 3.02 519 182 186 4 842.41 0.04 520 182 223 41 119.97 0.53 521 182 224 42 119.91 0.50 522 182 225 43 119.88 0.49 523 182 226 44 119.81 0.49 524 183 186 3 855.57 4.55 525 183 187 4 843.01 0.26 526 184 187 3 837.16 3.19 527 184 188 4 840.82 5.12 528 185 188 3 858.13 6.83 529 185 189 4 847.54 1.76 530 185 206 21 203.77 0.18 531 185 207 22 203.75 0.18 532 185 208 23 203.71 0.20 533 185 225 40 118.85 0.06 534 185 226 41 118.84 0.06 535 186 189 3 847.56 1.76 536 186 190 4 847.02 2.04 537 186 225 39 119.73 0.48 538 187 190 3 847.16 1.97 539 187 191 4 847.02 2.03 540 188 191 3 847.78 2.42 541 188 192 4 847.01 2.04 542 188 206 18 203.81 0.15 543 189 192 3 847.34 1.88 544 189 193 4 847.01 2.04 545 189 201 12 204.41 0.14 546 189 204 15 204.33 0.14 547 189 205 16 204.26 0.14 548 189 206 17 203.82 0.14 549 189 225 36 118.85 0.07 550 189 227 38 118.19 0.27 551 190 193 3 842.48 0.23 552 190 194 4 842.39 0.27 553 191 194 3 857.55 5.68 554 191 195 4 788.22 26.89 555 192 195 3 844.62 1.29 556 192 196 4 709.03 5.41 557 192 197 5 764.82 38.58 558 192 201 9 215.67 5.52 559 192 204 12 204.30 0.11 560 193 196 3 839.89 3.60 561 193 197 4 760.22 40.88 562 193 198 5 397.11 47.73 563 193 201 8 215.75 5.49 564 196 200 4 787.38 28.59 565 197 200 3 863.01 3.26 566 197 201 4 836.98 3.80 567 198 201 3 841.75 4.46 568 198 202 4 787.43 28.57 569 199 202 3 847.27 1.73 570 199 203 4 792.09 26.24 571 200 203 3 842.56 0.62 572 200 204 4 787.07 23.73 573 201 204 3 860.11 5.86 574 205 227 22 117.50 0.26 575 206 225 19 118.15 0.26 576 206 226 20 118.11 0.26 577 206 227 21 117.59 0.23 578 207 227 20 117.59 0.23 579 207 228 21 117.16 0.41 580 207 229 22 116.74 0.59 581 208 212 4 859.50 3.33 582 208 220 12 121.39 0.11 583 208 226 18 118.17 0.27 584 208 228 20 117.59 0.23 585 208 229 21 116.98 0.48 586 208 230 22 116.98 0.48 587 209 230 21 116.95 0.47 588 211 216 5 841.82 4.50 589 212 216 4 824.78 2.85 590 212 217 5 553.00 2.27 591 212 220 8 405.22 71.63 592 212 229 17 117.87 0.14 593 212 231 19 117.56 0.21 594 212 234 22 116.54 0.66 595 212 243 31 49.58 0.17 596 213 216 3 854.52 1.07 597 213 217 4 697.46 1.68 598 213 234 21 117.28 0.32 599 213 243 30 49.61 0.18 600 214 217 3 873.00 10.31 601 214 239 25 53.22 1.69 602 214 242 28 49.62 0.18 603 214 243 29 49.59 0.16 604 216 219 3 856.77 4.07 605 216 220 4 842.71 0.27 606 217 220 3 852.65 2.01 607 217 221 4 799.63 28.54 608 217 229 12 119.56 0.34 609 217 243 26 47.11 0.93 610 217 246 29 32.04 0.39 611 218 221 3 853.11 1.81 612 218 222 4 796.71 30.00 613 218 246 28 30.47 0.40 614 219 222 3 853.11 1.81 615 219 223 4 853.11 1.80 616 220 223 3 853.11 1.81 617 220 226 6 237.62 5.51 618 220 229 9 120.17 0.41 619 221 226 5 259.23 5.28 620 221 227 6 175.27 27.14 621 221 228 7 120.03 0.43 622 221 229 8 120.17 0.41 623 221 246 25 30.46 0.39 624 221 247 26 28.30 0.56 625 223 226 3 841.26 5.09 626 229 243 14 42.34 0.26 627 229 246 17 34.41 0.95 628 231 234 3 866.84 0.80 629 231 235 4 701.99 11.04 630 231 240 9 52.86 1.42 631 231 243 12 49.45 0.12 632 232 235 3 853.59 0.96 633 233 236 3 839.43 2.89 634 234 237 3 854.48 2.54 635 234 240 6 144.10 42.54 636 234 243 9 138.91 44.52 637 235 240 5 143.28 43.80 638 237 240 3 858.56 0.48 639 238 241 3 892.06 3.18 640 239 242 3 864.46 2.43 641 240 243 3 864.47 2.43 642 240 244 4 864.47 2.42 643 241 244 3 852.29 3.67 644 241 245 4 852.28 3.67 645 242 245 3 852.28 3.67 646 242 246 4 852.25 3.69 647 243 246 3 852.25 3.69 648 244 247 3 856.88 1.34 649 244 248 4 848.95 0.20 650 245 248 3 854.45 2.56