BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 7 3 838.46 9.59 2 4 8 4 840.47 8.58 3 4 134 130 106.34 0.95 4 4 135 131 110.80 1.23 5 4 138 134 106.50 0.87 6 4 139 135 101.65 0.30 7 4 140 136 98.23 1.01 8 4 174 170 98.21 1.02 9 5 8 3 865.55 3.96 10 5 9 4 840.47 8.58 11 5 174 169 98.21 1.02 12 5 196 191 111.92 0.41 13 5 198 193 98.22 1.01 14 6 9 3 872.44 3.48 15 6 10 4 847.00 2.18 16 7 10 3 872.44 3.48 17 7 11 4 847.00 2.18 18 7 138 131 114.42 0.72 19 7 140 133 113.75 0.51 20 8 11 3 872.44 3.48 21 8 12 4 847.00 2.17 22 8 76 68 113.40 0.45 23 8 140 132 113.78 0.53 24 8 142 134 113.74 0.51 25 8 175 167 113.74 0.51 26 8 200 192 113.54 0.41 27 8 226 218 113.67 0.32 28 9 12 3 853.99 1.87 29 9 13 4 807.90 2.68 30 9 14 5 716.00 48.66 31 9 198 189 114.07 0.44 32 9 226 217 113.51 0.40 33 10 14 4 714.84 49.25 34 11 74 63 114.92 0.51 35 11 107 96 114.95 0.51 36 12 18 6 114.94 0.51 37 12 20 8 114.92 0.51 38 12 56 44 114.92 0.51 39 12 57 45 114.92 0.51 40 12 74 62 114.95 0.49 41 12 76 64 114.98 0.51 42 12 107 95 115.00 0.51 43 12 108 96 115.00 0.51 44 13 16 3 829.44 4.09 45 13 17 4 114.96 0.52 46 13 18 5 114.95 0.51 47 13 226 213 115.01 0.51 48 15 54 39 546.16 28.28 49 15 56 41 494.98 23.50 50 15 252 237 27.06 1.05 51 16 226 210 163.47 0.07 52 17 221 204 163.46 0.07 53 17 225 208 163.53 0.07 54 17 226 209 163.47 0.07 55 17 251 234 27.15 1.04 56 17 252 235 27.10 1.05 57 18 56 38 546.02 28.21 58 18 200 182 163.60 0.03 59 18 226 208 163.61 0.03 60 18 227 209 163.47 0.07 61 18 228 210 163.47 0.07 62 18 251 233 27.15 1.04 63 18 252 234 27.11 1.05 64 19 55 36 546.23 28.28 65 19 56 37 546.22 28.28 66 19 57 38 546.19 28.26 67 19 228 209 163.50 0.08 68 19 230 211 163.45 0.09 69 19 247 228 41.70 0.15 70 19 248 229 38.29 0.20 71 19 251 232 27.12 1.05 72 19 252 233 27.11 1.05 73 20 56 36 546.24 28.29 74 20 57 37 546.19 28.26 75 20 58 38 546.14 28.25 76 20 59 39 546.12 28.25 77 20 76 56 544.42 29.02 78 20 200 180 163.94 0.07 79 20 228 208 163.66 0.04 80 20 229 209 163.50 0.09 81 20 230 210 163.50 0.09 82 21 46 25 546.61 28.36 83 21 49 28 546.92 28.44 84 21 50 29 546.45 28.32 85 21 55 34 546.43 28.30 86 21 57 36 546.25 28.29 87 21 58 37 546.21 28.27 88 21 59 38 546.19 28.26 89 21 230 209 163.60 0.03 90 21 231 210 163.46 0.09 91 21 234 213 148.69 0.11 92 21 235 214 148.58 0.15 93 22 25 3 972.83 0.65 94 22 59 37 546.20 28.26 95 22 230 208 163.66 0.04 96 22 231 209 163.48 0.08 97 22 235 213 149.78 0.44 98 23 34 11 547.52 28.41 99 23 40 17 547.23 28.47 100 23 41 18 547.23 28.47 101 23 42 19 547.13 28.46 102 23 43 20 546.99 28.43 103 23 46 23 547.00 28.44 104 23 59 36 546.35 28.32 105 23 60 37 546.34 28.31 106 23 64 41 546.35 28.31 107 23 235 212 148.69 0.11 108 24 27 3 875.68 1.48 109 24 28 4 832.22 6.61 110 24 59 35 546.45 28.34 111 24 61 37 546.34 28.31 112 24 78 54 546.32 28.31 113 25 28 3 845.40 9.79 114 25 29 4 716.88 52.37 115 25 30 5 557.52 27.31 116 25 33 8 549.58 27.77 117 25 231 206 164.46 0.16 118 25 232 207 163.79 0.06 119 25 235 210 163.43 0.10 120 26 30 4 554.88 28.63 121 26 34 8 549.74 27.73 122 27 61 34 547.01 28.46 123 27 81 54 546.75 28.46 124 27 82 55 547.00 28.52 125 27 83 56 547.04 28.50 126 28 78 50 547.07 28.49 127 28 81 53 546.98 28.52 128 28 82 54 547.00 28.52 129 28 83 55 547.02 28.52 130 28 84 56 547.06 28.50 131 29 33 4 853.65 0.14 132 29 84 55 547.06 28.50 133 29 232 203 165.65 0.08 134 30 33 3 892.02 2.35 135 31 34 3 870.98 0.75 136 31 35 4 867.62 2.43 137 32 35 3 870.98 0.75 138 33 232 199 164.97 0.04 139 33 235 202 163.69 0.04 140 33 236 203 162.95 0.01 141 34 37 3 871.28 1.07 142 34 38 4 691.35 36.69 143 34 40 6 547.49 28.48 144 34 41 7 547.24 28.48 145 34 235 201 163.66 0.05 146 35 38 3 846.32 3.43 147 36 206 170 164.50 0.18 148 36 236 200 163.65 0.05 149 36 238 202 162.35 0.04 150 37 40 3 865.51 2.68 151 37 45 8 693.31 38.48 152 37 235 198 163.67 0.04 153 37 236 199 162.65 0.29 154 37 237 200 162.31 0.46 155 37 238 201 154.03 0.24 156 38 41 3 865.62 2.63 157 39 42 3 865.21 2.83 158 39 45 6 693.15 38.40 159 40 45 5 723.25 26.73 160 40 46 6 696.52 40.09 161 40 235 195 161.87 0.24 162 42 45 3 858.21 2.79 163 42 46 4 854.28 0.96 164 43 46 3 854.29 0.96 165 43 47 4 859.48 0.36 166 43 64 21 550.24 28.91 167 43 68 25 550.66 28.91 168 44 47 3 861.37 0.58 169 44 48 4 855.56 1.60 170 44 68 24 550.81 28.88 171 45 48 3 855.35 3.58 172 45 49 4 833.06 0.58 173 45 240 195 92.61 12.79 174 46 49 3 852.62 3.06 175 46 50 4 843.76 3.50 176 46 58 12 551.00 28.88 177 46 60 14 550.91 28.83 178 46 235 189 151.72 1.39 179 47 50 3 862.89 4.67 180 47 51 4 840.85 2.04 181 47 58 11 550.90 28.82 182 47 60 13 550.91 28.82 183 47 68 21 551.24 28.80 184 47 69 22 551.28 28.82 185 47 70 23 551.58 28.94 186 47 72 25 551.63 28.96 187 48 51 3 848.62 1.07 188 48 52 4 834.11 1.32 189 49 52 3 860.43 5.91 190 49 53 4 834.92 1.76 191 49 234 185 158.34 1.46 192 49 235 186 148.80 0.20 193 49 240 191 56.76 2.95 194 49 241 192 56.66 2.94 195 50 54 4 824.78 6.83 196 50 55 5 555.08 27.23 197 50 58 8 551.25 28.80 198 50 73 23 551.63 28.97 199 51 54 3 841.18 2.18 200 51 72 21 569.52 25.95 201 51 73 22 551.75 28.91 202 52 241 189 56.68 2.94 203 52 245 193 52.30 1.73 204 53 241 188 56.61 2.91 205 53 244 191 50.97 1.74 206 53 245 192 50.80 1.66 207 53 248 195 41.19 0.33 208 54 252 198 28.22 0.60 209 55 234 179 149.25 0.38 210 55 244 189 50.84 1.67 211 55 248 193 37.64 0.52 212 55 252 197 27.30 0.96 213 56 73 17 676.71 29.54 214 56 74 18 676.51 29.51 215 56 252 196 27.14 1.03 216 57 73 16 676.78 29.51 217 57 74 17 676.55 29.50 218 58 73 15 676.89 29.54 219 58 74 16 676.82 29.50 220 58 75 17 676.66 29.51 221 58 76 18 676.56 29.50 222 59 76 17 676.70 29.54 223 59 78 19 676.54 29.50 224 60 64 4 847.96 4.88 225 60 65 5 819.54 2.51 226 60 69 9 677.13 29.50 227 60 75 15 676.90 29.53 228 60 76 16 676.81 29.53 229 60 77 17 676.77 29.50 230 60 78 18 676.74 29.52 231 61 64 3 870.72 3.12 232 61 65 4 852.62 2.54 233 61 78 17 676.77 29.50 234 61 80 19 676.77 29.50 235 61 81 20 676.75 29.50 236 61 95 34 676.79 29.51 237 62 65 3 856.03 0.84 238 62 66 4 851.16 3.27 239 62 77 15 676.90 29.53 240 62 95 33 676.82 29.52 241 62 96 34 676.82 29.52 242 62 100 38 676.87 29.53 243 62 104 42 676.98 29.55 244 63 66 3 863.49 2.89 245 63 67 4 842.55 1.03 246 64 67 3 863.17 2.71 247 64 68 4 833.94 5.33 248 64 69 5 827.38 5.34 249 65 68 3 856.49 1.08 250 65 69 4 820.30 1.80 251 65 75 10 676.99 29.55 252 65 77 12 676.90 29.52 253 65 104 39 677.00 29.55 254 66 104 38 677.01 29.55 255 69 73 4 892.62 2.69 256 69 74 5 851.11 2.76 257 69 75 6 676.98 29.55 258 69 104 35 677.24 29.52 259 69 105 36 677.24 29.52 260 74 107 33 743.39 26.22 261 75 104 29 743.85 26.13 262 75 105 30 743.75 26.14 263 75 106 31 743.54 26.21 264 75 107 32 743.49 26.23 265 75 108 33 743.43 26.22 266 76 108 32 743.42 26.21 267 76 109 33 743.39 26.22 268 76 110 34 743.36 26.21 269 76 142 66 589.47 50.73 270 77 96 19 744.12 26.14 271 77 101 24 743.90 26.09 272 77 104 27 743.98 26.09 273 77 106 29 743.77 26.16 274 77 108 31 743.66 26.19 275 77 109 32 743.44 26.21 276 77 110 33 743.43 26.21 277 78 110 32 743.44 26.21 278 78 111 33 743.43 26.21 279 78 112 34 743.43 26.21 280 78 144 66 589.50 50.75 281 79 94 15 744.19 26.14 282 79 95 16 744.21 26.14 283 79 96 17 744.15 26.12 284 79 97 18 744.12 26.14 285 79 98 19 744.09 26.14 286 79 101 22 744.11 26.14 287 79 109 30 743.78 26.15 288 79 110 31 743.74 26.14 289 79 111 32 743.48 26.20 290 79 130 51 590.38 50.66 291 80 92 12 744.32 26.18 292 80 93 13 744.26 26.17 293 80 94 14 744.20 26.14 294 80 95 15 744.21 26.14 295 80 111 31 743.96 26.04 296 80 112 32 743.96 26.03 297 81 92 11 744.33 26.18 298 81 93 12 744.33 26.18 299 81 95 14 744.27 26.16 300 82 86 4 856.02 2.87 301 82 91 9 744.35 26.18 302 82 92 10 744.34 26.18 303 82 114 32 744.06 26.01 304 83 86 3 874.71 6.47 305 86 90 4 878.84 1.23 306 86 91 5 825.52 0.34 307 86 119 33 773.19 10.53 308 89 117 28 773.86 10.86 309 89 118 29 772.10 9.98 310 89 119 30 770.69 9.28 311 89 149 60 582.86 54.02 312 89 150 61 582.86 54.02 313 90 114 24 774.12 10.98 314 90 117 27 772.17 10.01 315 90 119 29 758.69 3.21 316 90 123 33 709.27 21.45 317 90 148 58 584.54 53.22 318 90 149 59 582.65 53.93 319 91 114 23 774.13 10.95 320 91 119 28 701.41 25.43 321 91 122 31 690.65 30.80 322 91 123 32 686.84 32.66 323 92 111 19 774.22 10.96 324 92 114 22 774.15 10.94 325 92 123 31 621.84 65.22 326 93 122 29 621.90 65.20 327 93 126 33 621.74 65.27 328 94 111 17 774.20 10.94 329 94 126 32 621.74 65.28 330 94 130 36 620.48 65.67 331 96 100 4 840.70 3.60 332 96 101 5 832.79 0.35 333 96 104 8 810.32 5.83 334 97 100 3 848.51 0.29 335 97 101 4 853.13 2.62 336 98 101 3 853.13 2.62 337 98 102 4 853.14 2.62 338 98 109 11 774.35 10.94 339 98 130 32 620.48 65.67 340 98 133 35 617.85 64.55 341 98 134 36 617.78 64.52 342 98 137 39 613.21 62.23 343 98 139 41 610.26 60.84 344 99 102 3 859.70 0.65 345 99 103 4 853.14 2.63 346 99 137 38 613.31 62.29 347 100 103 3 856.88 0.76 348 100 104 4 853.12 2.64 349 101 104 3 872.93 8.25 350 101 105 4 840.26 3.72 351 101 106 5 808.36 5.83 352 101 109 8 774.34 10.93 353 101 139 38 610.24 60.88 354 102 105 3 865.26 4.69 355 102 137 35 613.29 62.30 356 102 139 37 613.16 62.34 357 106 139 33 613.14 62.35 358 107 139 32 610.21 60.88 359 107 140 33 610.18 60.88 360 108 139 31 609.95 60.76 361 108 140 32 602.06 56.83 362 108 142 34 590.62 51.26 363 109 134 25 613.38 62.47 364 109 139 30 604.62 58.09 365 109 140 31 597.68 54.62 366 109 141 32 590.45 51.15 367 109 142 33 590.41 51.16 368 110 142 32 590.34 51.16 369 111 123 12 622.25 65.47 370 111 126 15 621.07 65.88 371 111 127 16 620.95 65.86 372 111 130 19 620.10 65.64 373 111 141 30 590.56 51.08 374 111 142 31 590.25 51.00 375 111 143 32 589.87 50.93 376 111 144 33 589.85 50.91 377 112 144 32 589.85 50.91 378 112 146 34 589.81 50.91 379 113 123 10 622.26 65.46 380 113 124 11 622.26 65.46 381 113 127 14 621.94 65.60 382 113 143 30 590.08 50.84 383 113 144 31 589.91 50.90 384 113 145 32 589.85 50.91 385 113 146 33 589.83 50.92 386 113 167 54 169.43 0.42 387 113 171 58 169.42 0.44 388 114 117 3 860.05 1.91 389 114 120 6 691.31 31.10 390 114 123 9 691.18 31.07 391 114 146 32 589.66 50.86 392 114 147 33 589.55 50.81 393 114 148 34 586.01 52.52 394 115 120 5 710.19 21.68 395 115 145 30 589.93 50.88 396 115 146 31 589.84 50.91 397 115 147 32 589.63 50.86 398 115 148 33 589.63 50.86 399 115 167 52 169.53 0.40 400 116 120 4 831.00 1.07 401 116 149 33 589.46 50.87 402 116 150 34 581.23 53.13 403 117 120 3 845.22 2.12 404 117 123 6 838.01 1.79 405 117 148 31 589.41 50.84 406 117 149 32 586.63 52.02 407 117 150 33 584.26 53.05 408 118 121 3 950.72 0.65 409 119 122 3 866.50 1.30 410 119 123 4 856.40 1.44 411 120 123 3 864.53 0.32 412 120 124 4 852.29 1.64 413 121 124 3 854.52 0.52 414 121 125 4 851.00 1.00 415 122 125 3 854.51 0.53 416 122 126 4 843.60 0.25 417 123 126 3 848.81 2.33 418 123 127 4 843.93 4.44 419 124 127 3 864.18 8.64 420 124 128 4 847.95 2.81 421 124 167 43 169.53 0.40 422 124 171 47 169.31 0.49 423 125 128 3 868.31 6.58 424 125 129 4 868.31 6.58 425 126 129 3 870.57 5.45 426 126 130 4 853.10 0.22 427 127 130 3 846.84 2.91 428 127 131 4 846.84 2.91 429 127 141 14 590.70 51.02 430 127 143 16 590.26 50.91 431 127 171 44 168.70 0.56 432 128 131 3 872.70 10.02 433 128 132 4 847.38 2.64 434 129 132 3 858.29 2.81 435 129 133 4 847.38 2.64 436 130 133 3 872.57 3.64 437 130 134 4 847.03 2.76 438 130 141 11 590.69 51.02 439 131 134 3 864.55 0.38 440 131 135 4 864.55 0.38 441 131 141 10 590.71 51.02 442 131 172 41 167.97 0.26 443 131 174 43 167.94 0.26 444 132 135 3 864.56 0.38 445 132 136 4 842.77 0.62 446 133 136 3 848.63 2.30 447 133 137 4 840.63 0.95 448 134 137 3 856.63 1.70 449 134 138 4 748.12 33.37 450 134 139 5 675.55 28.19 451 134 141 7 590.71 51.02 452 135 138 3 844.45 2.56 453 140 174 34 167.12 0.23 454 141 173 32 167.90 0.24 455 141 174 33 167.12 0.24 456 141 175 34 167.12 0.23 457 142 175 33 167.10 0.22 458 142 176 34 167.04 0.24 459 142 177 35 167.03 0.24 460 143 168 25 168.00 0.21 461 143 173 30 167.94 0.25 462 143 175 32 167.65 0.14 463 143 176 33 167.09 0.22 464 143 177 34 167.06 0.24 465 144 177 33 167.04 0.24 466 145 164 19 168.11 0.14 467 145 168 23 168.69 0.44 468 145 177 32 167.53 0.07 469 145 178 33 167.47 0.05 470 146 177 31 168.57 0.39 471 146 178 32 167.98 0.18 472 146 179 33 167.90 0.17 473 146 202 56 167.93 0.21 474 147 152 5 190.97 0.49 475 147 155 8 168.35 0.27 476 147 160 13 168.74 0.41 477 147 164 17 168.78 0.40 478 148 152 4 803.37 8.79 479 148 155 7 168.26 0.20 480 148 179 31 168.78 0.40 481 149 152 3 858.54 2.81 482 152 155 3 894.34 2.62 483 152 160 8 172.37 0.22 484 154 157 3 849.78 3.53 485 154 160 6 717.38 38.99 486 155 160 5 717.38 38.99 487 155 161 6 720.01 37.68 488 155 179 24 172.47 0.20 489 156 161 5 800.48 2.55 490 156 179 23 172.88 0.31 491 156 180 24 172.57 0.15 492 156 181 25 172.47 0.20 493 157 160 3 868.88 1.67 494 157 161 4 857.22 7.51 495 158 161 3 874.66 1.21 496 158 162 4 848.28 4.17 497 158 181 23 177.04 0.95 498 158 186 28 182.69 1.79 499 159 162 3 868.88 1.68 500 159 163 4 864.43 3.90 501 160 163 3 864.81 3.71 502 160 164 4 857.23 7.50 503 161 164 3 857.72 0.87 504 161 165 4 846.99 6.26 505 161 178 17 188.16 1.20 506 161 179 18 188.12 1.20 507 161 181 20 188.21 1.21 508 161 187 26 188.41 1.26 509 161 190 29 188.59 1.26 510 162 165 3 871.06 3.00 511 162 166 4 849.66 4.92 512 162 189 27 188.60 1.24 513 162 190 28 188.55 1.24 514 162 193 31 189.20 1.05 515 163 166 3 861.04 1.56 516 163 167 4 849.78 4.87 517 164 167 3 865.62 1.03 518 164 168 4 871.30 5.87 519 164 178 14 188.30 1.17 520 165 168 3 874.33 4.36 521 165 169 4 874.34 4.35 522 165 176 11 189.09 1.06 523 165 178 13 188.57 1.26 524 165 190 25 189.09 1.06 525 165 193 28 201.13 6.98 526 165 194 29 189.33 1.07 527 166 169 3 874.35 4.34 528 166 170 4 864.62 0.52 529 166 193 27 201.13 6.98 530 167 170 3 864.63 0.52 531 167 171 4 852.86 6.39 532 168 171 3 877.25 1.38 533 168 172 4 864.62 0.51 534 168 173 5 718.31 45.85 535 168 176 8 189.11 1.05 536 169 172 3 872.83 3.59 537 169 176 7 201.30 6.97 538 169 193 24 238.23 2.60 539 169 194 25 225.03 4.87 540 169 197 28 219.67 2.21 541 173 176 3 884.90 5.25 542 173 197 24 291.79 0.57 543 174 197 23 291.80 0.57 544 174 198 24 291.80 0.57 545 175 197 22 291.82 0.58 546 175 198 23 291.79 0.57 547 176 194 18 292.01 0.57 548 176 197 21 291.96 0.58 549 176 198 22 291.83 0.58 550 176 199 23 291.76 0.57 551 176 200 24 291.76 0.57 552 177 200 23 291.76 0.57 553 177 202 25 291.72 0.58 554 177 229 52 291.62 0.59 555 178 187 9 295.37 1.58 556 178 190 12 292.28 0.62 557 178 199 21 291.96 0.58 558 178 200 22 291.93 0.58 559 178 201 23 291.80 0.58 560 178 202 24 291.77 0.57 561 179 187 8 336.87 22.33 562 179 202 23 291.81 0.58 563 180 183 3 951.41 1.12 564 180 184 4 891.32 4.09 565 180 187 7 336.88 22.33 566 180 201 21 291.95 0.58 567 180 216 36 291.93 0.55 568 180 219 39 292.00 0.57 569 180 220 40 292.00 0.57 570 181 186 5 707.49 42.40 571 181 187 6 410.04 57.52 572 183 186 3 878.75 2.21 573 183 187 4 839.25 6.62 574 184 187 3 853.11 1.22 575 184 188 4 839.25 6.62 576 184 219 35 292.17 0.60 577 184 222 38 292.70 0.52 578 184 223 39 292.70 0.52 579 185 188 3 849.91 3.64 580 185 189 4 852.97 5.17 581 186 189 3 852.20 2.50 582 186 190 4 849.92 3.64 583 187 190 3 859.19 2.02 584 187 191 4 849.89 3.63 585 187 199 12 293.00 0.57 586 187 201 14 292.50 0.44 587 187 220 33 292.69 0.46 588 187 223 36 292.92 0.52 589 187 225 38 293.01 0.56 590 188 191 3 849.64 4.83 591 188 192 4 846.06 3.16 592 188 223 35 292.81 0.57 593 189 192 3 848.53 4.28 594 189 193 4 847.36 1.70 595 190 193 3 853.93 1.58 596 190 194 4 853.93 1.58 597 191 194 3 858.88 2.02 598 191 195 4 853.93 1.58 599 191 199 8 293.04 0.57 600 191 223 32 293.08 0.57 601 191 224 33 293.08 0.57 602 191 225 34 293.07 0.58 603 192 195 3 854.96 1.05 604 194 197 3 840.85 3.45 605 194 199 5 333.09 20.57 606 198 226 28 373.14 0.70 607 199 225 26 373.29 0.74 608 199 226 27 373.23 0.73 609 199 227 28 373.15 0.70 610 200 226 26 373.31 0.73 611 200 227 27 373.16 0.71 612 200 228 28 373.12 0.71 613 200 229 29 373.11 0.71 614 201 213 12 373.55 0.74 615 201 216 15 373.44 0.72 616 201 220 19 373.39 0.74 617 201 227 26 373.32 0.73 618 201 228 27 373.14 0.71 619 201 229 28 373.12 0.71 620 202 229 27 373.23 0.74 621 203 213 10 373.55 0.73 622 203 229 26 373.33 0.73 623 203 230 27 373.14 0.71 624 203 233 30 371.11 0.26 625 204 232 28 373.28 0.71 626 204 233 29 373.13 0.72 627 205 232 27 373.29 0.71 628 205 233 28 373.21 0.71 629 206 232 26 373.29 0.71 630 206 233 27 373.15 0.71 631 206 236 30 364.16 2.33 632 206 237 31 358.05 0.80 633 207 212 5 804.43 1.70 634 207 213 6 567.12 96.03 635 207 216 9 382.14 3.49 636 207 233 26 373.27 0.70 637 207 237 30 369.53 0.36 638 208 212 4 853.82 4.83 639 209 212 3 859.69 1.89 640 209 213 4 853.82 4.83 641 210 213 3 859.69 1.89 642 210 214 4 853.82 4.83 643 210 237 27 359.35 0.97 644 210 239 29 345.68 0.40 645 210 243 33 59.59 1.27 646 211 214 3 863.28 0.10 647 211 215 4 853.82 4.83 648 212 215 3 865.76 3.45 649 212 216 4 843.92 0.10 650 213 216 3 865.59 6.54 651 213 217 4 843.91 0.10 652 213 228 15 373.47 0.78 653 213 230 17 373.39 0.74 654 213 233 20 373.12 0.71 655 213 243 30 58.51 1.82 656 213 247 34 50.47 0.07 657 214 217 3 844.79 3.86 658 214 218 4 838.35 0.98 659 214 243 29 58.37 1.89 660 214 247 33 50.47 0.07 661 214 250 36 40.91 0.05 662 215 218 3 844.68 1.99 663 215 219 4 844.68 2.00 664 216 219 3 861.52 5.17 665 216 220 4 844.69 2.00 666 217 220 3 844.69 2.00 667 217 221 4 843.52 2.58 668 217 228 11 373.49 0.78 669 217 247 30 46.80 0.02 670 217 250 33 39.33 0.24 671 217 251 34 37.01 0.83 672 218 221 3 863.86 5.83 673 218 222 4 834.05 2.14 674 218 250 32 39.32 0.24 675 219 222 3 837.90 0.21 676 219 223 4 830.70 3.81 677 220 223 3 849.81 5.34 678 220 224 4 831.59 3.76 679 220 225 5 648.55 75.60 680 220 227 7 373.87 0.67 681 220 228 8 373.87 0.66 682 221 224 3 832.69 3.21 683 221 227 6 373.87 0.66 684 221 250 29 37.02 0.83 685 221 251 30 35.72 1.21 686 228 247 19 44.72 0.51 687 230 233 3 889.15 3.25 688 230 234 4 850.47 4.58 689 231 234 3 879.75 1.24 690 231 235 4 844.30 1.50 691 232 235 3 865.94 1.71 692 232 236 4 648.12 67.05 693 233 237 4 842.04 0.39 694 233 243 10 57.77 2.19 695 234 237 3 861.41 2.23 696 234 240 6 56.77 2.93 697 234 244 10 52.88 1.36 698 234 247 13 50.45 0.07 699 235 240 5 120.62 34.87 700 237 240 3 728.23 42.09 701 237 243 6 581.02 100.53 702 239 242 3 853.37 2.08 703 239 243 4 847.44 0.01 704 240 243 3 868.23 10.35 705 240 244 4 847.43 0.01 706 241 244 3 853.45 2.98 707 241 245 4 847.43 0.01 708 242 245 3 867.05 8.92 709 242 246 4 843.19 2.10 710 243 246 3 849.78 0.61 711 243 247 4 837.99 0.51 712 244 247 3 851.45 5.30 713 244 248 4 832.16 3.41 714 245 248 3 832.12 4.37 715 245 249 4 823.49 7.75 716 246 249 3 840.68 0.14 717 246 250 4 839.94 0.49 718 247 250 3 840.22 0.04 719 247 251 4 820.31 9.30 720 248 251 3 838.80 0.76 721 248 252 4 799.26 0.23