BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 16 140 138 261.09 0.18 2 16 142 140 310.18 2.40 3 16 143 141 313.25 3.00 4 16 144 142 313.38 3.04 5 16 156 154 289.87 7.73 6 16 158 156 253.96 3.35 7 16 189 191 44.59 0.18 8 16 190 192 35.74 0.79 9 16 194 196 35.74 0.80 10 17 143 140 313.40 3.04 11 17 144 141 313.39 3.04 12 17 145 142 313.48 3.02 13 17 146 143 313.75 3.10 14 17 187 188 47.40 1.12 15 17 188 189 47.04 1.05 16 17 189 190 46.54 1.15 17 17 191 192 46.73 1.21 18 17 220 220 46.88 1.13 19 17 221 221 47.00 1.07 20 18 156 152 313.81 3.12 21 18 188 188 47.16 1.06 22 19 157 152 312.57 2.90 23 19 158 153 273.44 2.19 24 19 188 187 47.52 1.06 25 20 156 150 319.97 0.25 26 20 157 151 313.70 3.19 27 21 144 137 330.25 2.17 28 21 152 145 334.41 0.18 29 21 154 147 334.16 0.31 30 21 155 148 329.94 1.84 31 21 156 149 320.11 0.18 32 22 26 4 826.52 1.11 33 22 154 146 335.63 0.43 34 22 155 147 334.20 0.27 35 22 156 148 326.53 0.15 36 22 157 149 325.94 0.23 37 23 26 3 853.57 3.04 38 23 71 56 475.51 1.70 39 23 154 145 335.76 0.45 40 23 155 146 335.51 0.37 41 24 27 3 841.45 4.33 42 24 28 4 798.26 5.11 43 24 30 6 756.06 9.35 44 24 69 53 475.12 1.87 45 24 70 54 475.53 1.71 46 24 71 55 475.56 1.70 47 24 117 107 474.76 1.70 48 24 155 145 335.74 0.42 49 25 28 3 836.39 4.67 50 25 116 105 475.19 1.91 51 25 117 106 475.13 1.88 52 26 157 145 335.69 0.41 53 27 30 3 846.44 0.98 54 27 137 124 335.55 0.48 55 27 139 126 336.75 0.07 56 28 117 103 475.77 1.75 57 28 118 104 475.73 1.76 58 28 119 105 475.72 1.76 59 29 45 20 553.48 1.11 60 29 46 21 546.98 1.05 61 29 119 104 475.74 1.76 62 29 120 105 475.72 1.76 63 29 121 106 475.61 1.75 64 29 198 187 210.63 3.46 65 29 200 189 194.94 0.74 66 30 40 14 626.89 23.85 67 30 43 17 562.90 2.73 68 30 44 18 561.12 1.94 69 30 139 123 363.97 0.37 70 30 140 124 371.37 1.09 71 30 141 125 373.22 0.30 72 30 155 139 373.10 0.32 73 30 197 185 215.26 3.86 74 30 198 186 214.33 3.51 75 31 43 16 568.09 0.15 76 31 44 17 561.14 1.95 77 31 45 18 561.16 1.96 78 31 52 25 561.08 1.93 79 31 66 43 617.70 0.02 80 31 67 44 617.68 0.03 81 31 68 45 617.68 0.03 82 32 40 12 626.84 23.88 83 32 42 14 623.44 24.61 84 32 66 42 618.07 0.14 85 32 67 43 618.02 0.14 86 32 141 123 373.24 0.30 87 33 40 11 626.90 23.84 88 33 41 12 626.59 23.94 89 33 42 13 625.30 23.69 90 33 43 14 622.56 24.16 91 33 44 15 622.56 24.16 92 33 54 25 571.08 1.18 93 33 59 30 578.45 0.38 94 33 64 39 618.08 0.14 95 33 65 40 618.09 0.14 96 33 66 41 618.08 0.14 97 33 106 83 570.43 0.95 98 34 38 8 641.22 18.04 99 34 39 9 633.65 20.48 100 34 40 10 626.90 23.84 101 34 41 11 626.83 23.88 102 34 64 38 618.09 0.14 103 34 65 39 618.09 0.14 104 34 67 41 618.37 0.09 105 34 70 44 619.73 0.02 106 34 73 47 619.72 0.03 107 35 38 7 641.32 17.99 108 35 39 8 641.02 17.97 109 35 41 10 629.54 22.53 110 35 60 31 615.85 0.82 111 35 63 36 618.10 0.14 112 35 64 37 618.09 0.14 113 36 37 5 691.00 3.09 114 36 38 6 670.74 3.28 115 36 62 34 618.27 0.10 116 36 63 35 618.21 0.10 117 36 65 37 618.90 0.35 118 36 84 56 618.95 0.36 119 37 37 3 855.73 2.04 120 37 37 4 784.03 20.25 121 37 39 6 679.01 3.46 122 38 65 31 621.32 0.60 123 40 73 37 622.18 0.73 124 40 141 111 373.82 0.12 125 40 151 121 373.92 0.15 126 40 193 167 224.81 8.04 127 41 58 17 623.34 0.13 128 41 64 27 622.34 0.46 129 42 58 16 624.34 0.62 130 42 195 167 224.61 7.96 131 43 54 11 637.20 6.75 132 43 195 166 224.64 7.98 133 43 196 167 224.05 8.21 134 43 197 168 223.72 8.09 135 44 52 8 649.83 13.02 136 44 53 9 637.31 6.75 137 44 54 10 637.20 6.75 138 44 196 166 224.07 8.19 139 44 197 167 223.71 8.08 140 44 198 168 223.71 8.08 141 45 52 7 671.04 2.43 142 45 53 8 637.32 6.76 143 45 121 86 477.17 1.78 144 45 198 167 221.51 7.10 145 45 209 178 186.31 1.29 146 46 49 3 893.16 0.66 147 46 51 5 761.32 14.09 148 46 52 6 671.47 2.23 149 46 68 26 625.12 0.67 150 46 108 68 625.08 0.68 151 46 112 76 478.50 1.74 152 46 118 82 477.51 1.76 153 46 120 84 477.22 1.76 154 46 121 85 477.22 1.76 155 47 51 4 795.94 3.21 156 47 53 6 700.19 0.77 157 47 105 64 625.38 0.69 158 47 120 83 477.25 1.76 159 47 121 84 477.23 1.76 160 47 123 86 477.19 1.77 161 47 209 176 186.26 1.30 162 47 238 205 66.19 1.61 163 48 51 3 824.09 9.96 164 48 120 82 477.45 1.78 165 49 108 65 625.20 0.69 166 49 111 72 479.03 1.59 167 49 112 73 478.55 1.73 168 49 120 81 477.53 1.77 169 50 107 63 625.24 0.67 170 50 108 64 625.19 0.69 171 50 111 71 478.94 1.53 172 51 89 42 625.86 0.72 173 51 105 60 625.84 0.71 174 51 106 61 625.57 0.58 175 51 107 62 625.48 0.55 176 51 241 204 37.76 1.47 177 51 242 205 21.61 0.32 178 52 66 18 625.63 0.62 179 52 68 20 625.77 0.69 180 52 105 59 625.84 0.71 181 52 106 60 625.61 0.61 182 52 108 62 625.54 0.58 183 53 103 56 638.37 2.96 184 53 104 57 632.25 2.41 185 53 105 58 625.84 0.71 186 53 209 170 186.55 1.23 187 53 212 173 184.52 1.20 188 53 238 199 66.17 1.61 189 54 59 5 837.65 3.25 190 54 103 55 638.40 2.94 191 54 104 56 637.33 2.79 192 54 106 58 626.87 0.33 193 54 196 156 224.02 8.15 194 54 212 172 184.47 1.12 195 55 58 3 845.68 0.43 196 55 59 4 844.13 5.41 197 55 102 53 639.13 3.20 198 55 103 54 638.40 2.95 199 55 104 55 638.40 2.94 200 55 196 155 224.36 7.99 201 55 212 171 185.55 1.55 202 56 59 3 849.88 2.53 203 56 60 4 836.57 2.71 204 56 90 38 638.93 2.92 205 56 91 39 641.23 2.90 206 56 94 42 641.96 2.94 207 56 102 52 640.08 2.75 208 56 103 53 639.70 2.75 209 56 104 54 638.63 3.06 210 57 60 3 843.85 1.28 211 57 94 41 642.35 2.77 212 57 99 48 642.23 2.73 213 57 102 51 641.90 2.86 214 57 195 152 224.87 8.00 215 57 214 171 185.43 1.54 216 58 195 151 224.81 8.02 217 58 196 152 224.56 7.89 218 59 60 3 823.38 3.42 219 59 60 4 777.58 6.44 220 59 64 9 678.82 2.71 221 59 88 33 641.95 2.83 222 59 90 35 642.23 2.96 223 59 104 51 642.09 2.90 224 59 106 53 641.98 2.85 225 60 60 3 846.06 1.08 226 60 90 34 642.59 2.79 227 60 94 38 643.34 2.46 228 60 64 6 783.88 1.64 229 60 88 29 643.54 2.48 230 61 63 3 847.75 0.12 231 61 64 4 848.11 5.71 232 62 64 3 852.25 3.63 233 62 85 24 643.50 2.48 234 62 86 25 644.32 2.83 235 62 87 26 643.58 2.46 236 62 88 27 643.57 2.48 237 64 85 21 645.94 2.04 238 64 88 24 645.37 2.31 239 65 82 17 649.72 3.18 240 65 83 18 649.28 3.33 241 66 82 16 649.72 3.18 242 66 83 17 649.47 3.25 243 66 85 19 649.26 3.35 244 66 106 42 644.66 2.37 245 66 108 44 643.84 2.48 246 67 70 3 858.99 1.05 247 67 80 13 650.31 3.14 248 67 81 14 650.21 3.18 249 67 82 15 649.72 3.18 250 68 80 12 650.32 3.14 251 68 81 13 650.23 3.18 252 68 83 15 650.18 3.20 253 68 108 42 644.39 2.25 254 68 112 50 478.19 1.84 255 68 118 56 477.28 1.77 256 69 77 8 653.70 4.71 257 69 80 11 650.35 3.15 258 69 117 54 477.27 1.79 259 69 118 55 477.24 1.77 260 70 73 3 921.65 0.52 261 70 74 4 887.31 2.06 262 70 77 7 653.63 4.69 263 70 80 10 650.51 3.23 264 70 141 77 373.38 0.30 265 71 77 6 690.26 3.20 266 71 141 76 373.49 0.26 267 71 154 89 373.38 0.30 268 71 155 90 372.97 0.35 269 72 75 3 861.80 1.88 270 72 77 5 764.93 3.62 271 72 141 75 373.51 0.24 272 72 153 87 373.57 0.24 273 72 154 88 373.41 0.30 274 73 141 74 373.53 0.24 275 73 151 84 373.88 0.15 276 73 152 85 373.82 0.18 277 73 153 86 373.68 0.24 278 74 153 85 373.81 0.18 279 76 80 4 840.30 0.89 280 77 80 3 846.65 2.27 281 79 118 45 477.36 1.79 282 81 118 43 477.40 1.78 283 82 110 30 643.74 2.52 284 83 108 27 644.06 2.38 285 83 109 28 643.93 2.44 286 83 110 29 643.61 2.54 287 83 111 34 479.28 1.65 288 83 112 35 479.26 1.65 289 84 108 26 644.27 2.29 290 84 109 27 644.21 2.30 291 85 88 3 880.17 1.63 292 85 106 23 644.84 2.38 293 85 107 24 644.77 2.35 294 86 107 23 644.82 2.37 295 86 109 25 644.73 2.37 296 87 106 21 645.00 2.42 297 87 107 22 644.83 2.34 298 88 105 19 645.04 2.42 299 88 106 20 645.00 2.41 300 89 104 17 645.08 2.43 301 89 105 18 645.07 2.43 302 89 237 158 69.43 1.16 303 89 241 162 23.09 0.30 304 90 104 16 645.08 2.43 305 91 94 3 838.08 6.09 306 91 101 12 646.14 2.37 307 91 103 14 645.34 2.40 308 91 234 153 99.70 0.77 309 91 237 156 71.64 0.05 310 93 101 10 646.25 2.37 311 94 99 7 679.00 1.66 312 94 100 8 656.75 6.21 313 94 101 9 652.79 5.64 314 94 102 10 646.22 2.39 315 95 99 6 687.86 2.77 316 95 100 7 656.98 6.20 317 96 98 4 779.41 1.11 318 96 99 5 687.87 2.77 319 97 98 3 808.02 4.48 320 97 175 84 231.61 7.11 321 97 215 126 186.58 1.23 322 98 175 83 231.56 7.08 323 98 180 88 231.41 7.09 324 98 215 125 186.36 1.29 325 99 102 3 853.07 0.17 326 99 180 87 231.06 6.96 327 99 214 123 186.40 1.27 328 99 215 124 186.34 1.27 329 100 175 81 231.62 7.11 330 100 180 86 230.11 6.73 331 101 179 84 230.01 6.77 332 101 234 141 102.01 0.06 333 102 214 120 185.44 1.54 334 102 229 135 120.97 0.18 335 103 212 117 186.26 1.30 336 103 229 134 120.29 0.47 337 103 234 139 93.00 0.29 338 103 237 142 71.64 0.05 339 103 238 143 68.00 0.82 340 104 237 141 71.64 0.06 341 105 237 140 68.06 0.81 342 105 238 141 66.18 1.60 343 105 241 144 23.08 0.30 344 105 242 145 23.04 0.32 345 108 112 8 564.09 40.93 346 109 110 4 739.24 13.61 347 109 110 5 674.47 5.07 348 110 110 3 815.05 0.15 349 110 110 4 744.51 10.97 350 112 118 6 700.37 6.40 351 114 118 4 754.10 4.87 352 115 118 3 823.62 9.21 353 115 119 4 789.47 1.24 354 121 200 83 201.75 6.09 355 121 207 90 186.62 1.18 356 121 208 91 186.27 1.28 357 121 209 92 186.10 1.23 358 122 206 88 186.78 1.18 359 122 207 89 186.66 1.19 360 122 208 90 186.30 1.27 361 122 209 91 186.19 1.26 362 123 208 89 186.64 1.19 363 123 209 90 186.56 1.20 364 123 231 112 102.17 0.26 365 123 235 116 81.24 0.18 366 123 238 119 67.81 0.83 367 124 128 4 793.37 5.88 368 124 203 83 191.61 1.07 369 124 208 88 186.72 1.18 370 124 209 89 186.64 1.19 371 124 210 90 186.57 1.19 372 124 231 111 102.18 0.26 373 124 232 112 101.19 0.35 374 124 235 115 87.75 1.73 375 125 128 3 827.90 0.59 376 125 235 114 92.16 0.12 377 126 235 113 95.31 0.33 378 126 236 114 93.29 0.68 379 126 239 117 78.86 0.73 380 127 232 109 102.53 0.12 381 127 233 110 102.40 0.18 382 128 203 79 214.98 12.69 383 128 204 80 214.97 12.69 384 129 203 78 215.00 12.70 385 129 230 105 119.40 0.06 386 129 232 107 102.42 0.20 387 130 134 4 802.73 7.80 388 130 162 32 373.28 0.42 389 130 201 75 216.75 12.04 390 130 202 76 215.16 12.77 391 130 203 77 214.89 12.76 392 130 210 84 186.78 1.19 393 131 134 3 855.03 4.96 394 131 162 31 373.31 0.41 395 132 162 30 373.26 0.42 396 132 163 31 373.25 0.42 397 132 164 32 373.24 0.42 398 132 165 33 373.24 0.42 399 132 181 51 229.71 7.01 400 133 162 29 373.32 0.41 401 134 161 27 373.51 0.38 402 134 162 28 373.45 0.39 403 134 201 71 224.18 8.36 404 134 202 72 222.41 9.14 405 135 159 24 374.20 0.21 406 135 160 25 374.08 0.17 407 136 159 23 374.26 0.19 408 136 160 24 374.09 0.17 409 136 162 26 373.88 0.20 410 136 199 67 224.15 8.27 411 136 200 68 224.08 8.25 412 136 201 69 222.51 8.88 413 137 158 21 374.32 0.21 414 137 159 22 374.26 0.20 415 137 199 66 224.12 8.23 416 137 200 67 223.88 8.29 417 137 207 74 186.85 1.19 418 138 157 19 374.47 0.24 419 138 158 20 374.43 0.23 420 138 160 22 374.31 0.24 421 138 189 55 225.56 8.01 422 138 190 56 225.52 8.01 423 138 198 64 224.12 8.23 424 138 199 65 223.98 8.23 425 138 228 94 120.96 0.15 426 139 155 16 375.02 0.18 427 139 156 17 374.65 0.29 428 139 157 18 374.48 0.24 429 139 198 63 224.11 8.24 430 140 155 15 375.03 0.19 431 140 156 16 374.99 0.20 432 140 194 58 224.83 8.06 433 141 151 10 376.85 0.36 434 141 152 11 376.35 0.58 435 141 153 12 376.34 0.58 436 141 154 13 376.25 0.62 437 141 155 14 375.06 0.18 438 141 194 57 224.83 8.06 439 142 151 9 376.88 0.35 440 142 152 10 376.86 0.35 441 142 192 54 225.34 7.96 442 142 193 55 224.87 8.08 443 142 194 56 224.83 8.06 444 143 146 3 866.61 5.32 445 143 148 5 754.47 8.50 446 143 151 8 376.89 0.34 447 143 152 9 376.89 0.34 448 143 191 52 225.41 7.97 449 143 192 53 225.36 7.95 450 144 148 4 816.37 2.30 451 144 152 8 376.91 0.33 452 144 156 12 376.25 0.63 453 145 148 3 841.57 5.81 454 146 191 49 225.54 7.91 455 146 220 77 138.58 1.31 456 151 192 45 225.37 7.96 457 151 193 46 224.88 8.07 458 152 156 4 843.02 1.86 459 158 188 34 225.62 7.98 460 159 188 33 225.74 8.03 461 160 183 25 228.36 6.99 462 160 184 26 227.84 7.06 463 160 185 27 226.78 7.58 464 160 188 32 225.82 8.01 465 160 199 43 224.21 8.23 466 161 183 24 228.49 7.02 467 161 184 25 227.88 7.04 468 161 185 27 227.44 7.27 469 162 181 21 229.06 6.94 470 162 182 22 228.88 6.88 471 162 183 23 228.49 7.02 472 162 199 41 224.47 8.36 473 163 167 4 828.86 4.47 474 163 168 5 811.52 2.68 475 163 182 21 228.94 6.90 476 163 183 22 228.66 6.97 477 163 184 23 228.74 6.98 478 163 225 66 125.20 0.31 479 164 167 3 838.32 0.26 480 164 168 4 820.57 1.83 481 165 168 3 822.08 1.08 482 165 169 4 811.55 2.67 483 165 177 14 462.02 107.74 484 165 178 15 231.11 7.24 485 165 180 17 231.05 7.21 486 165 181 18 229.75 7.00 487 165 230 69 125.03 0.30 488 166 169 3 839.01 0.61 489 166 170 4 820.56 1.84 490 167 170 3 847.57 4.89 491 167 170 5 809.06 1.40 492 167 171 6 764.80 2.15 493 168 171 5 761.56 3.75 494 168 172 6 761.54 3.75 495 168 176 10 710.16 7.48 496 168 182 16 229.56 6.91 497 168 225 61 126.64 0.18 498 169 173 6 717.54 3.79 499 169 176 9 462.45 107.95 500 169 177 10 462.05 107.75 501 170 171 3 831.98 3.49 502 170 172 4 778.27 11.82 503 170 172 3 826.13 4.65 504 170 223 55 138.98 1.29 505 171 186 17 229.50 6.88 506 171 223 54 138.82 1.37 507 171 224 55 138.81 1.37 508 171 225 56 138.80 1.36 509 172 175 3 909.32 1.08 510 172 176 4 832.21 2.52 511 172 215 45 186.81 1.21 512 172 216 46 186.59 1.24 513 172 217 47 186.24 1.31 514 172 224 54 138.80 1.36 515 172 225 55 138.79 1.35 516 172 227 57 137.18 1.11 517 173 176 3 861.06 2.04 518 175 180 5 822.55 2.93 519 175 215 42 186.78 1.24 520 176 180 4 807.90 1.78 521 176 215 41 186.74 1.23 522 177 180 3 828.00 1.86 523 178 230 54 125.08 0.31 524 178 233 57 120.95 0.14 525 179 229 52 125.09 0.31 526 179 230 53 124.59 0.32 527 179 233 56 110.20 2.12 528 179 234 57 110.20 2.12 529 180 215 37 186.34 1.38 530 180 227 49 125.14 0.32 531 180 228 50 125.09 0.31 532 180 230 52 121.66 0.49 533 181 199 20 224.98 8.09 534 181 210 31 186.88 1.21 535 181 227 48 125.06 0.30 536 181 228 49 124.91 0.37 537 181 230 51 120.94 0.15 538 182 225 45 125.20 0.31 539 182 226 46 125.15 0.30 540 182 227 47 124.91 0.36 541 183 199 18 224.69 8.23 542 183 225 44 125.14 0.29 543 183 226 45 125.06 0.30 544 183 228 47 124.78 0.42 545 184 186 4 788.61 7.51 546 184 225 43 125.12 0.29 547 185 186 3 811.24 3.81 548 185 187 4 788.60 7.51 549 185 188 5 736.38 7.85 550 186 221 34 138.84 1.36 551 186 222 35 138.84 1.36 552 186 223 36 138.62 1.29 553 186 223 37 138.62 1.29 554 186 224 38 138.28 1.28 555 186 225 39 127.12 0.21 556 187 221 33 138.84 1.36 557 189 220 30 138.84 1.38 558 189 221 31 138.74 1.33 559 189 226 37 124.69 0.29 560 190 194 4 760.09 6.28 561 190 213 23 184.33 1.36 562 190 220 29 138.83 1.38 563 190 228 38 123.36 0.03 564 191 194 3 823.38 0.27 565 191 220 28 138.72 1.35 566 192 220 27 138.75 1.36 567 194 197 3 836.44 1.21 568 194 213 19 180.83 0.49 569 195 213 18 180.85 0.50 570 196 212 16 184.53 1.11 571 196 213 17 180.85 0.50 572 197 212 15 181.46 0.42 573 197 213 16 179.06 0.67 574 198 209 11 186.47 1.27 575 198 211 13 185.60 1.54 576 199 209 10 186.59 1.21 577 199 210 11 186.30 1.33 578 199 211 12 185.56 1.57 579 199 228 29 120.30 0.47 580 200 207 7 202.00 8.76 581 200 208 8 201.94 8.75 582 201 206 5 737.27 3.71 583 201 207 6 670.83 1.19 584 201 208 7 202.00 8.76 585 201 210 9 186.24 1.28 586 202 205 3 837.05 1.78 587 202 206 4 735.01 2.87 588 203 206 3 837.05 1.78 589 203 208 5 716.54 5.00 590 209 231 22 102.17 0.26 591 210 230 20 103.62 0.45 592 210 231 21 102.17 0.26 593 211 228 17 120.28 0.47 594 211 229 18 119.27 0.21 595 211 231 20 102.41 0.14 596 212 228 16 120.30 0.47 597 212 229 17 119.74 0.56 598 212 231 19 102.28 0.07 599 213 228 15 120.30 0.47 600 214 227 13 123.80 0.41 601 214 228 14 121.39 0.04 602 214 229 15 120.96 0.15 603 215 226 11 125.09 0.27 604 215 227 12 124.86 0.37 605 217 222 4 820.11 1.20 606 217 224 7 130.82 1.65 607 219 222 3 834.40 0.63 608 222 224 3 813.45 4.55 609 229 234 5 829.77 3.55 610 230 233 3 846.84 4.98 611 230 234 4 833.98 1.46 612 231 234 3 853.43 1.68 613 231 235 4 831.81 2.54 614 231 238 7 800.41 0.96 615 232 235 3 841.20 5.07 616 234 237 3 836.50 0.97 617 234 238 4 818.89 1.41 618 235 238 3 830.53 3.92 619 235 239 4 818.84 1.38 620 236 239 3 830.59 3.94 621 236 240 4 824.70 1.01 622 237 240 3 835.90 3.25 623 237 241 4 824.79 0.96 624 238 241 3 839.72 1.28 625 238 242 4 824.74 0.94 626 239 242 3 831.87 2.43 627 239 243 4 828.86 3.93 628 240 243 3 842.50 0.11