BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 66 65 295.75 0.56 2 1 67 66 293.03 0.07 3 2 65 63 296.20 0.54 4 2 66 64 295.86 0.51 5 2 68 66 295.75 0.56 6 2 69 67 295.03 0.90 7 2 70 68 293.13 0.04 8 2 71 69 293.01 0.02 9 2 72 70 292.97 0.01 10 2 94 92 217.21 0.24 11 3 63 60 297.43 0.53 12 3 64 61 296.69 0.57 13 3 65 62 296.31 0.49 14 3 94 91 218.25 0.61 15 3 95 92 217.05 0.29 16 3 96 93 216.61 0.50 17 3 97 94 216.21 0.42 18 3 98 95 216.21 0.42 19 3 99 96 215.38 0.29 20 4 63 59 297.85 0.33 21 4 64 60 296.98 0.50 22 4 66 62 296.41 0.47 23 4 72 68 292.97 0.03 24 4 75 71 292.95 0.03 25 4 93 89 218.58 0.61 26 4 94 90 218.33 0.64 27 4 95 91 217.43 0.20 28 5 61 56 300.53 0.65 29 5 62 57 299.68 1.00 30 5 92 87 218.97 0.62 31 5 93 88 218.83 0.66 32 5 95 90 218.42 0.61 33 5 99 94 216.46 0.51 34 6 32 26 300.23 0.89 35 6 36 30 299.67 1.00 36 6 61 55 301.39 1.07 37 6 62 56 299.81 0.99 38 6 64 58 299.68 0.99 39 6 75 69 292.98 0.01 40 6 79 73 292.58 0.10 41 6 89 83 253.70 2.35 42 6 91 85 226.00 1.35 43 6 92 86 218.94 0.60 44 7 59 52 302.85 1.24 45 7 60 53 302.12 0.82 46 7 61 54 300.90 0.77 47 7 89 82 257.87 0.68 48 7 90 83 250.22 0.67 49 7 91 84 241.06 0.93 50 8 32 24 302.00 0.86 51 8 58 50 338.92 10.74 52 8 59 51 313.50 0.55 53 8 60 52 303.50 1.50 54 8 62 54 302.13 0.81 55 8 87 79 264.05 0.61 56 8 88 80 263.81 0.53 57 8 89 81 261.83 1.06 58 8 90 82 257.87 0.68 59 9 57 48 357.95 1.22 60 9 58 49 338.92 10.74 61 9 87 78 265.56 0.14 62 9 88 79 263.99 0.59 63 9 90 81 263.24 0.42 64 10 15 5 814.91 6.87 65 10 18 8 422.84 1.95 66 10 28 18 322.74 2.72 67 10 51 41 375.94 1.16 68 10 55 45 382.13 0.38 69 10 56 46 376.05 1.06 70 10 57 47 362.73 0.41 71 10 60 50 356.05 2.21 72 10 86 76 284.80 0.27 73 10 87 77 267.69 1.20 74 11 15 4 864.00 4.87 75 11 56 45 376.08 1.07 76 11 86 75 287.97 1.28 77 11 127 116 208.19 0.55 78 12 15 3 864.00 4.87 79 12 54 42 386.12 2.35 80 12 55 43 382.20 0.41 81 12 56 44 380.22 1.32 82 13 54 41 390.31 3.06 83 13 56 43 396.31 0.48 84 13 126 113 208.31 0.61 85 13 127 114 208.34 0.60 86 14 26 12 465.79 6.30 87 14 86 72 291.40 0.06 88 15 26 11 465.79 6.31 89 15 27 12 465.77 6.30 90 15 28 13 465.67 6.19 91 15 31 16 465.44 6.15 92 15 53 38 413.80 1.06 93 15 54 39 413.79 1.06 94 15 86 71 291.36 0.04 95 15 87 72 291.25 0.08 96 16 21 5 749.05 12.54 97 16 25 9 465.95 6.29 98 16 26 10 465.95 6.28 99 16 27 11 465.95 6.28 100 16 53 37 413.80 1.06 101 17 21 4 854.86 0.46 102 17 27 10 466.07 6.21 103 17 28 11 466.09 6.22 104 17 51 34 413.91 1.04 105 17 52 35 413.88 1.05 106 17 53 36 413.88 1.05 107 18 27 9 466.10 6.22 108 18 28 10 466.10 6.21 109 18 29 11 466.10 6.22 110 18 47 29 414.36 1.09 111 18 49 31 414.26 1.12 112 18 50 32 413.96 1.03 113 18 51 33 413.94 1.03 114 18 60 42 413.80 1.09 115 18 62 44 413.67 1.10 116 19 27 8 466.19 6.18 117 19 29 10 466.14 6.20 118 19 47 28 414.42 1.07 119 19 49 30 414.36 1.08 120 19 50 31 413.97 1.03 121 20 27 7 466.25 6.17 122 20 29 9 466.18 6.19 123 20 30 10 466.13 6.21 124 20 33 13 466.17 6.19 125 21 25 4 816.83 16.09 126 21 27 6 467.76 5.42 127 22 25 3 877.11 0.79 128 26 31 5 802.38 10.35 129 26 84 58 292.10 0.17 130 26 86 60 291.49 0.01 131 27 30 3 818.53 16.46 132 27 31 4 849.93 0.77 133 28 31 3 867.74 2.38 134 28 32 4 847.69 1.89 135 28 62 34 413.71 1.08 136 28 87 59 266.50 0.38 137 29 32 3 867.74 2.38 138 29 33 4 847.15 1.62 139 29 45 16 414.56 1.13 140 30 33 3 849.99 0.82 141 30 34 4 847.70 1.89 142 31 34 3 858.78 3.33 143 31 35 4 862.35 1.54 144 31 83 52 291.27 0.09 145 31 84 53 291.25 0.08 146 31 87 56 272.14 0.91 147 32 35 3 862.35 1.54 148 32 36 4 862.35 1.54 149 32 45 13 414.86 1.24 150 32 62 30 413.74 1.08 151 32 79 47 292.62 0.12 152 32 83 51 291.22 0.10 153 32 87 55 266.48 0.36 154 33 36 3 868.69 1.79 155 33 37 4 869.34 4.08 156 33 43 10 414.87 1.24 157 33 44 11 414.89 1.24 158 33 45 12 414.87 1.25 159 34 37 3 914.47 0.01 160 35 38 3 879.49 0.55 161 35 79 44 292.58 0.14 162 35 82 47 291.20 0.09 163 35 83 48 291.12 0.09 164 36 39 3 872.95 1.75 165 36 40 4 841.34 5.94 166 36 41 5 741.86 28.41 167 36 42 6 443.25 14.91 168 36 43 7 416.51 1.85 169 36 44 8 416.51 1.85 170 36 45 9 416.47 1.88 171 36 64 28 413.52 1.17 172 36 79 43 291.95 0.24 173 37 40 3 818.75 11.51 174 37 41 4 759.06 19.80 175 37 43 6 416.81 1.72 176 38 82 44 291.13 0.08 177 39 66 27 413.43 1.15 178 39 68 29 413.30 1.18 179 39 70 31 400.36 6.00 180 39 75 36 292.99 0.01 181 39 78 39 291.95 0.24 182 39 79 40 291.86 0.21 183 40 66 26 413.43 1.15 184 40 67 27 413.38 1.15 185 40 68 28 413.22 1.14 186 41 65 24 413.50 1.18 187 41 66 25 413.38 1.15 188 41 67 26 413.38 1.15 189 42 65 23 413.45 1.16 190 42 67 25 413.39 1.15 191 44 64 20 413.58 1.11 192 45 62 17 413.78 1.08 193 45 63 18 413.73 1.10 194 45 64 19 413.50 1.10 195 46 62 16 413.80 1.09 196 46 63 17 413.74 1.11 197 46 100 54 216.18 0.42 198 46 101 55 215.96 0.49 199 46 102 56 215.43 0.26 200 47 60 13 413.88 1.07 201 47 61 14 413.81 1.09 202 47 62 15 413.69 1.13 203 47 101 54 216.12 0.46 204 47 102 55 215.96 0.53 205 48 60 12 413.88 1.07 206 48 61 13 413.81 1.09 207 48 101 53 216.18 0.42 208 48 102 54 215.43 0.26 209 48 108 60 208.13 0.58 210 48 244 196 42.18 0.15 211 48 246 198 39.85 0.55 212 49 60 11 413.88 1.07 213 50 110 60 208.38 0.59 214 50 195 145 208.05 0.61 215 51 55 4 758.22 24.28 216 51 60 9 413.88 1.10 217 51 241 190 66.81 0.53 218 52 55 3 867.99 5.55 219 52 199 147 208.19 0.59 220 53 236 183 77.96 0.33 221 54 236 182 77.77 0.26 222 54 237 183 77.41 0.09 223 55 199 144 208.17 0.56 224 55 236 181 77.62 0.21 225 55 239 184 70.53 0.43 226 56 126 70 208.31 0.61 227 56 127 71 208.25 0.58 228 56 237 181 77.27 0.07 229 56 239 183 70.70 0.35 230 56 240 184 67.49 0.48 231 56 241 185 67.47 0.47 232 57 60 3 868.19 3.10 233 57 241 184 66.08 0.22 234 58 90 32 263.90 0.61 235 58 111 53 208.04 0.49 236 58 240 182 67.51 0.48 237 58 241 183 67.45 0.49 238 58 242 184 62.33 1.38 239 59 241 182 66.41 0.06 240 59 242 183 62.07 1.27 241 59 243 184 50.45 0.81 242 60 241 181 62.55 1.28 243 61 241 180 63.98 0.79 244 61 244 183 42.03 0.22 245 61 246 185 39.02 0.53 246 62 101 39 215.91 0.56 247 63 99 36 216.24 0.42 248 63 100 37 216.16 0.43 249 63 101 38 215.18 0.29 250 66 70 4 860.88 0.88 251 70 74 4 863.09 2.06 252 70 75 5 841.22 1.10 253 70 78 8 770.97 16.73 254 71 74 3 871.96 0.36 255 71 75 4 867.53 0.16 256 72 75 3 881.74 4.37 257 72 76 4 885.11 2.69 258 72 92 20 220.09 0.12 259 72 93 21 219.22 0.48 260 72 94 22 218.77 0.69 261 73 76 3 935.68 1.49 262 74 77 3 886.96 1.78 263 74 78 4 853.65 1.02 264 75 78 3 862.77 5.13 265 75 79 4 852.05 0.23 266 75 92 17 228.72 2.65 267 76 79 3 863.66 2.32 268 76 80 4 823.90 14.04 269 76 89 13 261.51 1.51 270 76 91 15 245.53 0.29 271 76 92 16 238.56 2.18 272 77 80 3 855.93 1.55 273 77 81 4 846.94 0.64 274 78 81 3 883.15 3.27 275 78 82 4 851.43 2.90 276 79 82 3 856.92 2.11 277 79 83 4 839.15 5.05 278 79 89 10 262.10 1.23 279 80 83 3 854.94 1.14 280 80 88 8 263.58 0.58 281 80 89 9 263.49 0.62 282 83 87 4 817.22 16.14 283 83 88 5 747.39 8.14 284 83 89 6 747.39 8.14 285 84 87 3 894.21 3.46 286 84 88 4 869.94 0.62 287 85 88 3 931.19 3.77 288 95 99 4 746.88 20.57 289 96 99 3 835.58 4.03 290 99 104 5 716.01 13.11 291 99 246 147 37.69 0.04 292 100 103 3 854.73 3.09 293 100 104 4 746.48 11.02 294 101 105 4 835.33 10.80 295 101 246 145 38.77 0.41 296 102 105 3 862.86 0.97 297 102 108 6 791.37 24.58 298 104 246 142 39.33 0.33 299 104 247 143 38.76 0.35 300 105 108 3 931.22 1.54 301 105 245 140 42.08 0.24 302 105 246 141 39.38 0.31 303 106 191 85 579.38 0.21 304 106 245 139 42.48 0.30 305 106 246 140 41.75 0.40 306 106 247 141 39.54 0.39 307 107 169 62 580.08 0.04 308 107 189 82 580.09 0.04 309 107 190 83 579.88 0.05 310 107 191 84 579.39 0.21 311 107 243 136 61.17 2.23 312 107 244 137 45.27 0.51 313 107 245 138 44.10 0.41 314 108 189 81 580.11 0.03 315 108 190 82 580.08 0.03 316 108 191 83 580.06 0.01 317 108 192 84 579.84 0.04 318 108 193 85 579.51 0.20 319 108 241 133 67.48 0.49 320 108 243 135 59.20 1.27 321 108 244 136 47.23 0.09 322 109 187 78 580.39 0.04 323 109 188 79 580.20 0.04 324 109 189 80 580.11 0.03 325 109 241 132 67.49 0.49 326 109 242 133 67.38 0.53 327 109 243 134 62.58 1.54 328 110 187 77 580.47 0.07 329 110 188 78 580.23 0.04 330 110 193 83 580.11 0.03 331 110 198 88 579.47 0.15 332 110 239 129 79.61 2.07 333 110 240 130 75.24 3.35 334 110 241 131 67.50 0.48 335 110 242 132 67.39 0.53 336 111 185 74 580.85 0.05 337 111 186 75 580.56 0.07 338 111 187 76 580.48 0.07 339 111 239 128 80.36 1.78 340 111 240 129 79.22 2.12 341 111 242 131 69.96 0.71 342 112 129 17 759.66 40.23 343 112 185 73 580.94 0.01 344 112 186 74 580.56 0.07 345 112 202 90 579.46 0.15 346 112 234 122 116.42 0.23 347 112 235 123 116.39 0.21 348 112 238 126 86.78 1.23 349 112 239 127 80.38 1.79 350 113 124 11 759.87 40.27 351 113 127 14 759.73 40.23 352 113 128 15 759.70 40.22 353 113 129 16 759.66 40.23 354 113 183 70 581.50 0.14 355 113 184 71 581.09 0.01 356 113 185 72 580.95 0.01 357 113 238 125 86.80 1.24 358 113 240 127 86.88 1.19 359 114 129 15 759.66 40.23 360 114 131 17 759.60 40.20 361 114 135 21 652.41 35.04 362 114 140 26 581.27 0.10 363 114 183 69 581.58 0.14 364 114 184 70 581.18 0.03 365 114 186 72 581.04 0.01 366 114 206 92 577.48 1.10 367 115 121 6 759.89 40.27 368 115 124 9 759.88 40.27 369 115 128 13 759.76 40.23 370 115 129 14 759.73 40.23 371 115 130 15 759.70 40.22 372 115 131 16 759.69 40.22 373 115 180 65 617.50 1.91 374 115 181 66 603.51 1.55 375 115 182 67 594.47 6.04 376 115 183 68 581.64 0.11 377 116 131 15 759.70 40.22 378 116 132 16 759.68 40.21 379 116 133 17 759.61 40.20 380 116 134 18 748.33 36.77 381 116 135 19 717.04 36.80 382 116 181 65 613.98 3.68 383 116 182 66 594.49 6.05 384 116 184 68 594.46 6.05 385 117 120 3 941.42 2.20 386 117 121 4 875.71 1.76 387 117 130 13 759.76 40.23 388 117 131 14 759.77 40.23 389 117 132 15 759.70 40.22 390 117 180 63 629.62 0.13 391 117 181 64 624.70 2.49 392 118 130 12 759.87 40.25 393 118 132 14 759.75 40.24 394 119 130 11 759.87 40.25 395 119 132 13 759.78 40.23 396 120 130 10 759.86 40.24 397 121 124 3 849.27 1.49 398 121 125 4 810.87 14.84 399 122 125 3 847.00 2.62 400 124 127 3 854.49 4.62 401 124 128 4 835.26 2.66 402 124 180 56 624.72 2.52 403 125 237 112 116.45 0.25 404 126 237 111 116.10 0.31 405 127 237 110 115.79 0.46 406 127 238 111 115.45 0.41 407 127 240 113 115.36 0.39 408 128 237 109 115.76 0.48 409 128 238 110 113.03 0.20 410 129 206 77 579.41 0.15 411 129 235 106 116.50 0.24 412 129 237 108 116.30 0.29 413 129 238 109 114.84 0.71 414 131 135 4 871.81 2.13 415 131 206 75 579.46 0.17 416 131 210 79 575.25 0.63 417 133 210 77 575.25 0.62 418 135 140 5 849.77 1.87 419 135 184 49 676.56 12.32 420 135 206 71 579.37 0.18 421 135 209 74 573.13 0.35 422 135 210 75 569.37 0.27 423 136 139 3 864.31 0.21 424 136 140 4 849.88 1.81 425 136 209 73 569.44 0.30 426 137 140 3 859.04 2.75 427 137 141 4 849.88 1.81 428 137 173 36 680.19 13.87 429 137 182 45 692.87 13.11 430 137 184 47 692.18 13.39 431 138 141 3 879.75 1.35 432 138 142 4 849.78 1.86 433 139 142 3 849.88 1.82 434 139 143 4 849.75 1.88 435 139 208 69 575.35 0.42 436 139 215 76 153.08 0.55 437 140 143 3 846.51 0.17 438 140 144 4 843.32 1.32 439 140 173 33 692.96 13.05 440 140 186 46 694.56 13.87 441 140 205 65 578.14 0.21 442 140 206 66 578.07 0.24 443 140 208 68 569.44 0.30 444 140 209 69 562.79 1.71 445 141 144 3 848.97 4.14 446 141 145 4 843.31 1.31 447 141 172 31 692.96 13.05 448 141 173 32 692.96 13.05 449 142 145 3 865.51 3.95 450 142 146 4 854.81 2.75 451 142 215 73 152.88 0.65 452 143 146 3 859.26 0.53 453 143 147 4 854.81 2.75 454 143 205 62 577.23 0.25 455 143 208 65 569.12 0.17 456 143 215 72 151.72 0.53 457 143 218 75 150.67 0.58 458 144 147 3 866.47 1.26 459 144 148 4 858.81 0.76 460 144 171 27 696.96 13.71 461 144 172 28 703.40 16.12 462 144 173 29 705.14 16.93 463 144 186 42 703.47 16.16 464 144 188 44 697.00 13.70 465 144 205 61 576.05 0.77 466 145 148 3 874.25 5.15 467 146 151 5 867.99 2.75 468 146 215 69 152.43 0.87 469 146 216 70 151.68 0.52 470 146 218 72 150.38 0.71 471 146 219 73 148.60 0.95 472 147 151 4 873.47 0.01 473 147 153 6 765.59 8.72 474 147 156 9 689.38 15.92 475 147 168 21 706.51 16.47 476 147 201 54 579.48 0.15 477 147 205 58 576.05 0.77 478 147 218 71 149.64 0.75 479 147 222 75 140.58 0.24 480 148 168 20 706.50 16.48 481 148 169 21 706.71 16.44 482 148 170 22 706.57 16.51 483 148 171 23 706.51 16.54 484 149 153 4 969.42 0.71 485 149 167 18 706.73 16.43 486 149 168 19 706.69 16.41 487 150 166 16 706.79 16.39 488 150 167 17 706.75 16.43 489 151 165 14 706.88 16.40 490 151 166 15 706.84 16.41 491 151 222 71 138.39 0.09 492 152 164 12 706.90 16.39 493 152 165 13 706.88 16.40 494 152 225 73 129.59 0.45 495 153 156 3 866.02 3.71 496 153 157 4 809.10 15.52 497 153 164 11 706.95 16.41 498 153 166 13 707.02 16.46 499 153 168 15 707.08 16.48 500 153 194 41 706.83 16.46 501 153 197 44 666.59 9.29 502 153 201 48 593.14 4.58 503 153 222 69 138.31 0.18 504 154 157 3 809.10 15.52 505 154 160 6 711.85 14.31 506 154 164 10 707.03 16.45 507 154 194 40 706.91 16.43 508 154 225 71 129.55 0.47 509 155 221 66 140.02 0.84 510 155 225 70 129.59 0.45 511 155 226 71 129.52 0.46 512 155 227 72 129.09 0.45 513 155 230 75 127.43 0.21 514 156 196 40 706.79 16.43 515 156 197 41 701.50 15.38 516 156 200 44 593.54 4.75 517 156 201 45 593.53 4.76 518 156 204 48 592.71 4.73 519 156 218 62 149.32 0.59 520 156 221 65 140.03 0.84 521 156 222 66 139.96 0.86 522 157 160 3 883.43 0.38 523 157 194 37 707.04 16.50 524 157 195 38 706.91 16.47 525 157 196 39 706.74 16.45 526 157 197 40 705.53 16.17 527 157 227 70 129.09 0.45 528 158 195 37 707.03 16.50 529 158 196 38 706.74 16.45 530 158 227 69 129.09 0.44 531 159 195 36 707.06 16.50 532 160 194 34 707.08 16.50 533 160 195 35 707.07 16.50 534 162 166 4 973.85 3.65 535 162 193 31 707.15 16.48 536 163 194 31 707.07 16.53 537 164 194 30 707.02 16.55 538 166 192 26 707.24 16.54 539 166 193 27 707.17 16.56 540 166 194 28 707.11 16.59 541 167 190 23 707.54 16.46 542 167 191 24 707.46 16.48 543 168 171 3 924.56 4.00 544 168 188 20 707.97 16.48 545 168 189 21 707.93 16.48 546 168 190 22 707.54 16.46 547 168 193 25 707.47 16.48 548 168 201 33 589.19 5.00 549 169 189 20 707.96 16.48 550 169 190 21 707.84 16.52 551 169 191 22 707.88 16.50 552 170 187 17 708.02 16.48 553 170 188 18 708.00 16.48 554 170 189 19 707.96 16.48 555 171 187 16 708.02 16.49 556 171 188 17 708.01 16.48 557 172 186 14 708.11 16.50 558 172 187 15 708.08 16.48 559 173 184 11 708.65 16.46 560 173 185 12 708.50 16.45 561 173 186 13 708.11 16.50 562 174 183 9 708.90 16.48 563 174 184 10 708.66 16.46 564 174 185 11 708.51 16.46 565 175 182 7 708.99 16.50 566 175 183 8 708.90 16.48 567 175 184 9 708.66 16.46 568 176 182 6 709.00 16.50 569 176 183 7 708.95 16.49 570 177 182 5 708.99 16.50 571 178 181 3 830.09 14.78 572 186 202 16 579.46 0.15 573 186 205 19 576.02 0.76 574 188 193 5 865.16 1.43 575 188 198 10 579.49 0.17 576 188 201 13 579.47 0.15 577 188 202 14 579.44 0.15 578 188 205 17 576.04 0.76 579 189 193 4 904.83 3.35 580 190 193 3 918.40 1.05 581 193 197 4 772.85 10.43 582 193 198 5 766.24 7.12 583 193 201 8 760.72 1.73 584 194 197 3 808.91 19.33 585 196 199 3 869.07 7.15 586 196 200 4 809.93 18.02 587 197 200 3 853.89 3.32 588 197 201 4 856.74 1.90 589 198 201 3 857.86 2.46 590 198 202 4 856.73 1.90 591 198 239 41 76.25 3.83 592 199 202 3 856.74 1.90 593 199 203 4 813.36 19.79 594 199 233 34 116.43 0.22 595 199 234 35 116.38 0.21 596 199 235 36 116.39 0.21 597 199 236 37 115.12 0.78 598 199 239 40 86.55 1.31 599 200 203 3 857.85 2.44 600 200 204 4 820.61 16.16 601 200 233 33 116.42 0.22 602 201 204 3 864.57 0.91 603 201 205 4 807.68 16.95 604 202 205 3 847.35 1.70 605 202 206 4 808.38 16.60 606 202 234 32 116.42 0.23 607 203 206 3 846.38 1.34 608 203 207 4 817.40 12.18 609 203 232 29 117.23 0.42 610 203 234 31 116.42 0.23 611 204 207 3 850.89 1.41 612 204 208 4 817.41 12.19 613 204 212 8 732.60 16.16 614 204 218 14 150.94 0.44 615 204 221 17 147.44 1.24 616 204 230 26 127.62 0.37 617 204 231 27 122.73 1.28 618 205 208 3 829.65 18.32 619 205 209 4 812.61 14.59 620 205 218 13 150.95 0.44 621 206 209 3 818.86 17.71 622 206 210 4 812.60 14.59 623 207 210 3 808.55 16.62 624 207 211 4 801.43 16.43 625 207 212 5 729.80 14.73 626 207 231 24 126.87 0.74 627 207 232 25 126.88 0.74 628 208 211 3 823.28 15.42 629 208 212 4 774.39 2.95 630 208 215 7 155.74 2.06 631 208 218 10 152.37 0.89 632 212 217 5 814.01 13.60 633 212 221 9 770.43 9.79 634 212 229 17 129.06 0.42 635 213 217 4 809.41 11.30 636 214 217 3 885.05 0.29 637 215 218 3 891.40 2.61 638 216 219 3 881.11 2.54 639 217 220 3 885.25 0.47 640 217 221 4 861.42 0.53 641 218 221 3 832.80 14.84 642 218 222 4 813.40 12.80 643 219 222 3 862.19 1.22 644 219 223 4 812.86 13.07 645 220 223 3 854.91 4.86 646 220 224 4 806.18 16.41 647 220 229 9 130.68 0.90 648 221 224 3 864.39 0.96 649 221 225 4 792.87 10.35 650 221 229 8 129.10 0.44 651 221 230 9 128.68 0.34 652 222 225 3 832.93 14.79 653 224 229 5 813.36 5.01 654 226 229 3 871.91 6.74 655 227 230 3 847.73 0.26 656 235 238 3 888.26 2.33 657 236 239 3 856.07 0.68