BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 218 214 154.90 1.01 2 4 219 215 154.84 1.01 3 4 246 242 27.52 1.44 4 4 247 243 26.91 1.12 5 4 248 244 23.53 1.02 6 5 45 40 797.44 1.56 7 5 46 41 797.43 1.56 8 5 99 94 224.93 34.23 9 5 167 162 191.66 18.27 10 5 197 192 185.86 16.23 11 5 214 209 154.92 1.01 12 5 219 214 154.91 1.01 13 5 220 215 154.84 1.01 14 5 221 216 154.83 1.01 15 6 44 38 797.48 1.55 16 6 45 39 797.47 1.55 17 6 46 40 797.47 1.55 18 6 210 204 154.88 1.02 19 6 214 208 155.19 0.99 20 6 221 215 154.85 1.02 21 6 227 221 131.46 1.15 22 6 237 231 66.85 0.96 23 6 238 232 66.75 0.96 24 6 241 235 65.03 0.29 25 7 44 37 797.50 1.55 26 7 46 39 797.47 1.55 27 7 47 40 797.45 1.56 28 7 48 41 797.44 1.56 29 7 199 192 185.24 15.96 30 7 220 213 155.19 0.99 31 7 221 214 154.88 1.01 32 7 222 215 154.83 1.01 33 7 223 216 154.84 1.01 34 7 227 220 131.49 1.15 35 8 39 31 797.63 1.52 36 8 44 36 797.58 1.54 37 8 46 38 797.50 1.55 38 8 47 39 797.46 1.55 39 8 48 40 797.46 1.55 40 8 223 215 154.86 1.01 41 8 227 219 131.49 1.15 42 9 48 39 797.46 1.55 43 9 50 41 797.44 1.56 44 9 222 213 155.18 0.99 45 9 223 214 154.88 1.01 46 9 224 215 154.83 1.01 47 9 228 219 132.25 0.80 48 10 17 7 800.50 1.03 49 10 32 22 797.89 1.54 50 10 35 25 797.91 1.55 51 10 36 26 797.90 1.54 52 10 39 29 797.89 1.54 53 10 47 37 797.55 1.54 54 10 48 38 797.48 1.55 55 10 49 39 797.47 1.55 56 10 50 40 797.46 1.55 57 10 228 218 135.06 0.41 58 11 17 6 800.53 1.04 59 11 18 7 800.56 1.05 60 11 50 39 797.47 1.55 61 12 16 4 802.29 1.91 62 12 17 5 800.56 1.05 63 12 18 6 800.86 1.20 64 12 32 20 799.32 1.54 65 12 49 37 797.53 1.54 66 12 50 38 797.48 1.55 67 12 51 39 797.44 1.56 68 12 52 40 797.44 1.56 69 12 65 53 283.35 59.45 70 13 16 3 846.75 5.81 71 13 18 5 802.32 1.90 72 13 52 39 797.43 1.56 73 13 53 40 797.32 1.54 74 14 53 39 797.40 1.56 75 14 61 47 314.56 44.18 76 15 32 17 801.01 1.75 77 15 61 46 284.67 59.12 78 16 28 12 801.26 1.80 79 16 30 14 801.23 1.79 80 16 31 15 801.17 1.78 81 16 32 16 801.12 1.80 82 17 20 3 852.17 8.53 83 17 21 4 867.08 1.07 84 17 30 13 801.24 1.79 85 17 31 14 801.23 1.79 86 17 32 15 801.21 1.79 87 17 35 18 801.21 1.79 88 18 52 34 797.43 1.56 89 19 229 210 154.87 0.98 90 20 30 10 801.40 1.79 91 20 228 208 154.86 0.97 92 20 229 209 154.84 0.96 93 21 29 8 801.84 1.95 94 21 35 14 801.53 1.83 95 21 228 207 154.74 0.96 96 21 229 208 154.72 0.97 97 22 228 206 154.79 0.95 98 22 229 207 154.62 0.92 99 22 230 208 154.42 1.02 100 23 26 3 885.20 5.09 101 23 29 6 801.99 1.93 102 23 35 12 801.88 1.94 103 23 38 15 801.85 1.94 104 23 39 16 801.80 1.93 105 23 227 204 154.84 0.96 106 23 228 205 154.78 0.95 107 23 230 207 154.41 1.02 108 25 38 13 801.88 1.94 109 26 29 3 849.60 3.63 110 26 34 8 801.97 1.91 111 26 35 9 801.97 1.91 112 26 38 12 801.88 1.94 113 27 34 7 801.99 1.92 114 28 31 3 861.40 4.80 115 28 34 6 809.40 1.79 116 31 34 3 858.54 1.40 117 31 35 4 825.00 0.64 118 32 35 3 877.40 4.11 119 32 36 4 825.01 0.64 120 32 49 17 797.59 1.54 121 32 65 33 283.33 59.47 122 33 36 3 877.50 4.05 123 33 37 4 853.09 1.26 124 33 65 32 283.32 59.47 125 33 68 35 283.31 59.47 126 33 69 36 283.14 59.51 127 33 72 39 282.80 59.60 128 34 37 3 866.56 0.52 129 34 38 4 853.10 1.26 130 35 38 3 866.57 0.52 131 35 39 4 855.65 0.02 132 35 228 193 154.16 0.95 133 36 39 3 868.51 0.46 134 36 40 4 862.58 3.45 135 36 47 11 797.60 1.55 136 36 73 37 282.75 59.61 137 37 40 3 869.26 0.10 138 37 41 4 854.88 7.30 139 37 72 35 282.79 59.59 140 37 73 36 282.74 59.62 141 38 41 3 869.27 0.11 142 38 42 4 848.86 10.31 143 39 42 3 863.40 3.04 144 39 43 4 848.60 10.44 145 39 44 5 810.93 5.11 146 39 47 8 797.59 1.55 147 39 227 188 152.12 0.91 148 39 228 189 141.36 0.26 149 40 75 35 280.64 60.26 150 42 234 192 66.97 0.96 151 42 235 193 66.91 0.99 152 43 234 191 66.97 0.96 153 43 238 195 66.86 0.97 154 44 47 3 942.75 3.97 155 44 75 31 280.38 60.38 156 44 227 183 136.27 1.24 157 44 234 190 66.95 0.96 158 44 249 205 27.53 1.18 159 45 75 30 279.94 60.38 160 45 76 31 279.95 60.38 161 45 238 193 66.80 0.99 162 45 249 204 27.00 1.09 163 46 75 29 280.14 60.29 164 46 76 30 279.94 60.38 165 46 99 53 226.21 33.61 166 47 73 26 282.69 59.58 167 47 75 28 281.42 59.91 168 47 76 29 280.39 60.38 169 47 77 30 279.71 60.41 170 47 78 31 279.32 60.26 171 48 78 30 279.23 60.66 172 48 222 174 155.19 1.00 173 49 65 16 283.28 59.45 174 49 69 20 283.18 59.41 175 49 77 28 281.41 59.90 176 49 78 29 280.10 60.45 177 49 79 30 278.77 60.44 178 49 80 31 278.75 60.45 179 50 80 30 278.72 60.46 180 50 81 31 278.51 60.54 181 51 58 7 318.65 42.20 182 51 62 11 283.47 59.51 183 51 80 29 279.58 60.37 184 51 81 30 278.63 60.48 185 52 58 6 522.66 141.66 186 53 57 4 855.03 2.65 187 53 58 5 321.29 40.98 188 53 61 8 318.64 42.20 189 54 57 3 893.48 7.13 190 54 58 4 824.82 1.05 191 55 58 3 855.04 2.65 192 57 60 3 891.49 1.12 193 57 61 4 852.61 0.29 194 58 61 3 867.25 5.23 195 58 62 4 852.61 0.29 196 58 81 23 297.23 52.19 197 58 82 24 297.19 52.12 198 59 62 3 874.11 1.80 199 59 63 4 854.22 0.41 200 59 91 32 258.45 28.51 201 60 63 3 870.36 0.07 202 60 64 4 854.21 0.41 203 61 64 3 870.35 0.08 204 61 65 4 856.48 0.59 205 62 65 3 868.01 1.08 206 62 66 4 855.43 1.51 207 62 79 17 301.48 50.19 208 62 80 18 301.50 50.19 209 62 81 19 301.45 50.16 210 62 83 21 301.32 50.05 211 62 92 30 249.44 23.66 212 63 66 3 872.28 1.19 213 63 67 4 856.62 2.10 214 63 91 28 248.93 23.39 215 63 92 29 248.78 23.49 216 63 95 32 248.91 23.41 217 64 67 3 872.29 1.20 218 64 68 4 866.60 4.07 219 65 68 3 871.95 1.41 220 65 69 4 856.88 0.78 221 66 69 3 867.05 3.86 222 66 70 4 856.44 0.55 223 66 77 11 301.64 50.20 224 66 79 13 301.55 50.16 225 66 92 26 248.94 23.35 226 66 95 29 248.36 23.02 227 66 96 30 248.03 23.04 228 66 97 31 247.95 23.02 229 67 70 3 872.47 1.15 230 67 71 4 864.15 5.30 231 67 95 28 248.35 23.02 232 68 71 3 887.32 3.35 233 68 72 4 851.20 1.18 234 69 72 3 868.55 3.05 235 69 73 4 851.13 1.17 236 69 77 8 302.11 49.97 237 70 73 3 887.31 2.08 238 70 77 7 335.33 33.36 239 70 96 26 248.23 22.93 240 73 77 4 893.36 1.63 241 76 98 22 257.79 17.83 242 77 96 19 258.27 17.92 243 77 97 20 258.00 17.83 244 77 98 21 257.91 17.80 245 77 99 22 257.83 17.80 246 78 99 21 257.81 17.82 247 78 100 22 257.88 17.78 248 78 101 23 257.88 17.77 249 78 126 48 227.62 2.66 250 79 83 4 904.45 4.45 251 79 92 13 259.02 18.09 252 79 97 18 258.21 17.89 253 79 99 20 257.97 17.81 254 79 100 21 257.88 17.77 255 79 101 22 257.88 17.77 256 80 83 3 900.73 6.31 257 80 101 21 257.87 17.76 258 83 88 5 689.87 59.98 259 83 89 6 604.60 98.51 260 83 92 9 327.98 39.80 261 83 100 17 258.50 17.61 262 83 101 18 258.46 17.59 263 83 102 19 258.38 17.55 264 83 105 22 259.49 16.04 265 84 105 21 259.49 16.04 266 84 112 28 250.90 3.89 267 85 88 3 883.97 2.03 268 85 89 4 849.51 3.89 269 85 112 27 250.99 3.83 270 86 89 3 860.84 9.54 271 86 90 4 849.50 3.88 272 86 112 26 251.02 3.83 273 86 115 29 250.92 3.59 274 86 116 30 249.97 3.31 275 86 119 33 260.74 3.02 276 86 120 34 260.19 2.85 277 87 90 3 884.82 2.45 278 87 91 4 851.46 2.91 279 88 91 3 885.81 1.96 280 88 92 4 851.46 2.91 281 89 92 3 879.84 1.39 282 89 93 4 847.06 4.55 283 89 100 11 268.70 12.66 284 89 116 27 255.62 0.04 285 90 93 3 869.88 3.60 286 90 94 4 850.73 2.55 287 90 120 30 302.94 24.84 288 91 94 3 866.89 4.50 289 91 95 4 844.83 5.50 290 92 95 3 856.55 1.75 291 92 96 4 850.41 2.71 292 92 97 5 762.35 32.84 293 92 100 8 271.16 11.44 294 93 96 3 849.15 3.18 295 93 97 4 684.59 67.38 296 93 100 7 271.44 11.35 297 93 116 23 323.80 34.12 298 93 120 27 323.49 35.34 299 93 124 31 301.86 34.26 300 94 120 26 338.72 42.88 301 98 124 26 329.31 47.98 302 99 123 24 329.32 47.99 303 99 124 25 329.31 47.98 304 99 126 27 329.29 47.99 305 99 167 68 329.29 47.99 306 100 116 16 339.21 43.20 307 100 124 24 329.35 48.00 308 100 125 25 329.33 47.99 309 100 126 26 329.31 48.00 310 101 126 25 329.31 48.00 311 101 128 27 329.29 47.99 312 102 106 4 836.22 2.94 313 102 112 10 342.12 41.90 314 102 113 11 341.85 41.91 315 102 116 14 340.73 42.59 316 102 125 23 329.49 47.95 317 102 126 24 329.41 47.99 318 102 127 25 329.36 47.98 319 102 128 26 329.37 47.98 320 103 106 3 859.69 1.85 321 103 107 4 852.63 1.67 322 103 128 25 329.41 48.00 323 103 130 27 329.37 47.98 324 104 107 3 863.38 0.01 325 104 108 4 852.64 1.67 326 104 142 38 329.50 47.96 327 104 143 39 330.41 47.52 328 105 108 3 856.21 3.58 329 105 109 4 851.22 2.38 330 105 112 7 481.06 27.39 331 106 109 3 855.91 3.44 332 106 110 4 703.07 5.00 333 106 113 7 388.12 18.93 334 106 127 21 336.94 44.47 335 106 156 50 338.36 43.79 336 107 110 3 855.97 1.08 337 107 140 33 337.06 44.43 338 107 142 35 337.96 43.98 339 107 149 42 338.00 43.96 340 107 151 44 337.41 44.27 341 107 156 49 337.08 44.43 342 108 143 35 338.05 43.94 343 109 112 3 877.71 6.46 344 110 113 3 867.60 3.40 345 110 156 46 339.55 43.37 346 110 163 53 340.69 43.48 347 111 114 3 850.95 0.93 348 111 115 4 844.37 1.02 349 111 163 52 340.81 43.48 350 112 115 3 853.26 0.25 351 112 116 4 849.59 1.58 352 113 116 3 873.55 1.22 353 113 117 4 850.66 2.00 354 113 125 12 339.37 44.30 355 113 127 14 339.34 44.29 356 113 156 43 341.04 43.60 357 113 160 47 340.85 43.59 358 113 163 50 341.05 43.59 359 113 164 51 341.02 43.59 360 113 165 52 340.64 43.66 361 114 117 3 870.20 0.12 362 114 118 4 859.35 2.33 363 114 163 49 341.05 43.59 364 115 118 3 873.21 1.39 365 115 119 4 867.22 1.60 366 116 119 3 882.47 6.02 367 116 120 4 842.41 2.08 368 116 125 9 340.80 43.73 369 117 120 3 859.60 2.21 370 117 121 4 529.34 3.85 371 117 122 5 560.71 66.05 372 117 125 8 374.21 27.04 373 117 164 47 374.26 27.06 374 120 124 4 836.20 13.94 375 121 124 3 877.38 3.50 376 121 125 4 866.45 8.96 377 122 125 3 868.85 2.74 378 122 164 42 509.62 1.51 379 122 165 43 488.66 0.39 380 122 166 44 467.83 1.28 381 123 166 43 483.36 1.92 382 125 165 40 512.33 1.84 383 125 166 41 511.51 2.02 384 125 167 42 511.51 2.02 385 126 167 41 511.72 2.12 386 126 169 43 510.53 1.55 387 126 199 73 511.45 2.01 388 127 156 29 515.19 1.18 389 127 160 33 513.35 1.96 390 127 165 38 512.75 1.92 391 127 167 40 512.51 1.91 392 127 168 41 512.29 1.85 393 127 169 42 511.76 2.12 394 128 169 41 512.29 1.85 395 129 140 11 524.33 2.71 396 129 153 24 523.51 2.82 397 129 156 27 515.31 1.23 398 129 157 28 515.22 1.18 399 129 160 31 515.25 1.20 400 129 168 39 514.63 1.06 401 129 169 40 514.62 0.98 402 129 170 41 514.51 0.93 403 129 171 42 515.07 1.11 404 130 140 10 524.35 2.71 405 130 141 11 524.33 2.72 406 130 171 41 515.16 1.09 407 130 173 43 523.59 3.12 408 131 139 8 525.15 2.31 409 131 140 9 524.36 2.71 410 131 153 22 524.18 2.77 411 131 171 40 523.48 3.03 412 131 172 41 523.49 3.06 413 131 173 42 523.65 3.10 414 131 186 55 523.61 3.06 415 132 138 6 525.27 2.31 416 132 139 7 525.23 2.30 417 132 141 9 524.89 2.50 418 132 173 41 523.67 3.10 419 132 174 42 523.65 3.14 420 132 186 54 523.80 3.04 421 133 137 4 525.38 2.35 422 133 138 5 525.28 2.31 423 133 174 41 523.80 3.16 424 133 183 50 523.88 3.13 425 133 185 52 524.24 2.95 426 134 137 3 846.28 4.32 427 134 139 5 525.40 2.36 428 134 141 7 525.98 2.66 429 134 148 14 524.88 2.81 430 137 152 15 525.04 3.10 431 138 152 14 525.04 3.12 432 138 153 15 524.96 3.08 433 138 185 47 524.74 3.21 434 138 186 48 524.49 3.11 435 138 189 51 524.54 3.12 436 139 148 9 525.64 3.06 437 139 150 11 525.21 3.13 438 139 151 12 525.10 3.15 439 139 152 13 525.05 3.12 440 139 153 14 524.99 3.10 441 140 149 9 525.52 3.12 442 140 151 11 525.11 3.15 443 140 152 12 525.10 3.15 444 140 153 13 525.03 3.14 445 140 156 16 525.03 3.15 446 141 145 4 533.79 1.18 447 141 147 6 525.81 3.08 448 141 148 7 525.68 3.08 449 141 149 8 525.65 3.07 450 141 173 32 524.06 3.30 451 142 145 3 865.80 6.77 452 142 146 4 765.58 43.34 453 142 147 5 526.26 3.01 454 142 149 7 525.78 3.07 455 143 146 3 865.79 6.77 456 143 147 4 672.38 3.26 457 145 173 28 524.27 3.40 458 151 156 5 842.71 2.43 459 152 155 3 861.33 1.19 460 152 156 4 858.46 2.62 461 153 156 3 867.12 0.26 462 153 157 4 861.33 1.19 463 153 170 17 525.52 3.68 464 153 186 33 526.02 3.85 465 153 189 36 526.29 3.82 466 153 190 37 526.25 3.84 467 154 157 3 880.68 6.45 468 154 158 4 861.68 1.03 469 154 189 35 526.29 3.83 470 154 193 39 526.56 3.77 471 155 158 3 880.10 4.64 472 155 159 4 855.81 1.91 473 156 159 3 860.83 4.42 474 156 160 4 852.61 0.31 475 157 160 3 866.76 1.45 476 157 161 4 868.00 2.06 477 157 168 11 526.29 3.82 478 157 190 33 526.32 3.81 479 157 193 36 527.06 3.58 480 157 194 37 527.04 3.58 481 158 161 3 873.76 4.94 482 158 162 4 853.09 5.39 483 158 193 35 527.02 3.55 484 159 162 3 856.08 3.90 485 159 163 4 853.10 5.39 486 160 163 3 879.81 3.85 487 160 164 4 853.09 5.39 488 160 165 5 746.17 33.35 489 160 168 8 527.19 3.65 490 160 196 36 588.10 3.89 491 161 164 3 843.02 0.35 492 161 194 33 552.82 1.39 493 161 195 34 567.18 6.63 494 161 196 35 552.83 1.38 495 165 196 31 660.11 0.86 496 166 196 30 660.11 0.86 497 166 197 31 660.10 0.87 498 167 196 29 660.16 0.85 499 167 197 30 660.10 0.87 500 167 199 32 660.07 0.88 501 168 194 26 660.53 0.83 502 168 196 28 660.26 0.84 503 168 197 29 660.24 0.84 504 168 198 30 660.08 0.88 505 168 199 31 660.07 0.88 506 169 199 30 660.07 0.88 507 169 201 32 660.01 0.90 508 169 222 53 245.36 43.19 509 170 186 16 660.73 0.84 510 170 187 17 660.49 0.96 511 170 190 20 660.71 0.83 512 170 198 28 660.32 0.81 513 170 199 29 660.22 0.85 514 170 200 30 660.11 0.88 515 170 201 31 660.04 0.90 516 171 176 5 661.49 0.69 517 171 201 30 660.05 0.90 518 172 175 3 862.58 5.44 519 172 176 4 693.98 6.32 520 172 183 11 660.53 0.98 521 172 186 14 660.82 0.83 522 172 201 29 660.24 0.85 523 173 176 3 861.11 6.17 524 175 178 3 870.24 5.97 525 175 180 5 661.05 0.81 526 175 181 6 660.97 0.83 527 175 182 7 660.92 0.84 528 175 183 8 660.90 0.84 529 175 202 27 660.47 0.86 530 175 203 28 660.06 0.90 531 176 202 26 660.48 0.86 532 176 203 27 660.47 0.86 533 178 203 25 660.60 0.83 534 179 203 24 660.63 0.83 535 179 204 25 660.60 0.83 536 179 205 26 660.11 0.84 537 179 209 30 631.88 1.76 538 179 231 52 217.63 43.36 539 180 203 23 660.62 0.83 540 180 209 29 627.57 0.11 541 181 203 22 660.58 0.81 542 181 209 28 605.58 0.10 543 182 187 5 847.21 5.94 544 182 200 18 660.69 0.83 545 182 201 19 660.65 0.83 546 182 202 20 660.62 0.83 547 182 204 22 660.38 0.85 548 182 209 27 446.85 50.12 549 182 210 28 472.63 24.84 550 182 213 31 247.34 43.05 551 182 221 39 246.72 42.93 552 182 222 40 246.72 43.02 553 182 223 41 246.45 42.98 554 183 186 3 879.82 3.52 555 183 187 4 851.40 3.85 556 184 187 3 855.75 6.02 557 184 188 4 847.19 5.95 558 184 212 28 247.87 43.30 559 184 213 29 247.34 43.09 560 184 216 32 247.33 43.09 561 185 188 3 873.63 1.02 562 185 189 4 851.41 3.85 563 186 189 3 861.87 1.41 564 186 190 4 873.05 6.62 565 187 190 3 879.17 3.56 566 187 191 4 877.39 4.45 567 187 200 13 660.63 0.83 568 187 213 26 247.13 43.11 569 187 216 29 247.09 43.13 570 187 217 30 247.00 43.11 571 187 218 31 246.91 43.09 572 188 191 3 880.68 4.48 573 188 192 4 880.76 2.77 574 188 215 27 247.21 43.11 575 188 216 28 247.09 43.12 576 189 192 3 933.53 3.77 577 189 193 4 888.62 0.20 578 190 193 3 888.62 0.20 579 190 194 4 879.36 4.83 580 190 198 8 660.97 0.94 581 191 194 3 899.55 5.54 582 191 195 4 885.73 1.65 583 191 196 5 870.68 0.49 584 191 198 7 661.00 0.97 585 191 217 26 246.65 43.19 586 191 219 28 246.56 43.15 587 194 198 4 888.12 1.41 588 197 219 22 245.74 43.30 589 198 218 20 246.30 43.05 590 198 219 21 245.77 43.30 591 198 220 22 245.72 43.32 592 199 220 21 245.42 43.17 593 199 222 23 245.37 43.19 594 200 213 13 246.49 43.14 595 200 218 18 246.68 43.05 596 200 220 20 246.31 43.05 597 200 221 21 245.40 43.18 598 200 222 22 245.39 43.18 599 201 222 21 245.39 43.18 600 204 209 5 829.28 1.32 601 204 210 6 756.62 34.77 602 204 223 19 246.45 42.98 603 204 226 22 245.38 43.18 604 205 209 4 873.33 6.76 605 205 226 21 245.43 43.19 606 205 232 27 169.62 37.69 607 206 209 3 886.45 1.15 608 206 210 4 876.54 5.16 609 206 232 26 168.88 38.06 610 207 210 3 881.58 3.58 611 207 211 4 876.54 5.16 612 207 232 25 160.27 42.36 613 207 236 29 124.53 25.94 614 207 237 30 67.08 1.02 615 207 240 33 66.81 0.92 616 208 211 3 876.54 5.16 617 208 212 4 873.32 6.76 618 208 240 32 66.82 0.92 619 209 212 3 861.49 5.50 620 209 213 4 853.50 3.14 621 210 213 3 859.38 4.63 622 210 214 4 855.70 4.24 623 210 221 11 247.03 43.02 624 210 223 13 246.97 43.05 625 210 227 17 245.71 43.35 626 210 232 22 168.88 38.06 627 210 237 27 66.89 0.93 628 210 241 31 65.14 0.27 629 211 214 3 863.61 0.29 630 211 215 4 853.56 3.18 631 211 240 29 66.68 0.86 632 211 241 30 65.03 0.29 633 211 244 33 53.95 1.42 634 211 246 35 42.81 2.17 635 212 215 3 866.26 3.17 636 212 216 4 852.73 3.60 637 213 216 3 861.84 0.96 638 213 217 4 852.73 3.60 639 213 218 5 826.56 0.03 640 214 217 3 861.83 0.96 641 214 218 4 826.58 0.03 642 214 219 5 826.57 0.04 643 214 220 6 682.41 2.52 644 214 221 7 320.83 6.49 645 214 241 27 65.03 0.29 646 214 246 32 37.88 0.80 647 223 226 3 911.65 1.38 648 223 227 4 866.93 1.32 649 224 227 3 877.30 2.09 650 224 228 4 855.12 2.56 651 225 228 3 855.12 2.56 652 225 229 4 855.10 2.57 653 226 230 4 690.68 5.98 654 226 231 5 215.05 59.77 655 226 232 6 135.99 21.60 656 227 230 3 841.84 3.81 657 227 232 5 161.15 41.91 658 227 234 7 74.06 0.10 659 227 237 10 74.09 0.08 660 232 237 5 849.46 1.00 661 233 236 3 855.07 3.23 662 233 237 4 851.24 1.89 663 234 237 3 869.00 3.33 664 234 238 4 851.27 1.89 665 235 238 3 879.25 1.91 666 235 239 4 854.32 3.38 667 236 239 3 861.72 0.32 668 236 240 4 854.26 3.41 669 237 240 3 864.35 1.62 670 237 241 4 854.33 3.39 671 238 241 3 869.00 3.95 672 238 242 4 854.12 3.48 673 238 248 10 33.52 0.58 674 238 249 11 32.79 0.94 675 238 250 12 29.82 0.31 676 239 242 3 872.97 0.22 677 239 243 4 859.60 0.74 678 239 250 11 33.48 0.59 679 240 243 3 870.66 4.90 680 240 244 4 869.25 2.08 681 241 244 3 880.59 1.25 682 241 245 4 869.25 2.08 683 241 246 5 620.03 27.83 684 241 248 7 128.25 22.71 685 242 245 3 869.65 2.28 686 242 246 4 761.56 37.87 687 242 247 5 629.64 32.63 688 242 248 6 206.63 64.44 689 242 250 8 37.94 1.39