BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 169.17 1.56 2 3 204 201 169.16 1.56 3 3 205 202 167.75 0.96 4 3 227 224 112.80 0.17 5 4 34 30 806.77 0.54 6 4 35 31 806.76 0.55 7 4 36 32 806.72 0.56 8 4 37 33 806.67 0.57 9 4 38 34 806.66 0.57 10 4 205 201 169.17 1.56 11 5 37 32 806.71 0.56 12 5 38 33 806.70 0.56 13 5 221 216 113.06 0.18 14 5 226 221 113.11 0.19 15 5 227 222 112.95 0.20 16 5 247 242 20.63 0.01 17 6 37 31 806.72 0.56 18 6 38 32 806.70 0.56 19 6 40 34 806.69 0.57 20 6 90 84 194.49 0.12 21 6 124 118 170.26 1.53 22 6 161 155 169.95 1.66 23 6 205 199 170.16 1.44 24 6 207 201 169.03 1.50 25 6 227 221 113.37 0.14 26 6 228 222 112.95 0.19 27 6 229 223 112.95 0.19 28 7 37 30 806.77 0.54 29 7 38 31 806.74 0.56 30 7 39 32 806.70 0.56 31 7 40 33 806.70 0.56 32 7 229 222 112.98 0.18 33 7 240 233 55.52 0.90 34 7 243 236 42.52 0.04 35 7 244 237 35.63 0.22 36 7 247 240 24.36 0.31 37 8 40 32 806.70 0.56 38 8 42 34 806.69 0.57 39 8 229 221 113.28 0.15 40 8 230 222 112.94 0.19 41 8 231 223 112.94 0.19 42 8 240 232 55.54 0.91 43 9 40 31 806.74 0.56 44 9 41 32 806.70 0.56 45 9 42 33 806.70 0.57 46 9 231 222 112.96 0.20 47 9 235 226 80.24 0.18 48 9 240 231 55.54 0.91 49 10 42 32 806.71 0.56 50 10 64 54 460.84 2.52 51 10 92 82 197.13 0.30 52 10 95 85 193.33 1.09 53 10 230 220 114.14 0.12 54 10 231 221 113.27 0.14 55 10 232 222 112.92 0.19 56 10 235 225 85.90 0.93 57 11 14 3 853.98 2.86 58 11 20 9 807.85 0.39 59 11 23 12 807.61 0.51 60 11 24 13 807.55 0.54 61 11 27 16 807.55 0.53 62 11 41 30 806.77 0.55 63 11 42 31 806.74 0.55 64 11 43 32 806.70 0.56 65 11 51 40 632.46 27.12 66 11 64 53 461.03 2.62 67 11 235 224 106.41 0.91 68 11 236 225 85.36 1.20 69 12 44 32 806.73 0.56 70 12 64 52 460.97 2.59 71 12 95 83 197.20 0.34 72 12 97 85 196.91 0.48 73 12 232 220 114.09 0.10 74 13 44 31 806.74 0.55 75 14 44 30 806.75 0.55 76 14 236 222 112.74 0.21 77 15 19 4 836.23 0.01 78 15 20 5 829.01 1.83 79 15 47 32 806.37 0.56 80 16 19 3 846.43 3.20 81 16 20 4 837.18 0.45 82 16 47 31 806.36 0.54 83 17 20 3 849.36 0.07 84 17 21 4 838.79 1.24 85 17 50 33 753.58 9.06 86 18 21 3 842.48 2.73 87 18 22 4 841.49 2.23 88 19 22 3 854.77 4.41 89 19 23 4 839.37 1.27 90 20 23 3 848.52 3.06 91 20 24 4 839.05 1.11 92 20 50 30 713.10 0.75 93 20 51 31 633.19 27.49 94 20 54 34 568.40 50.78 95 21 24 3 858.24 1.58 96 21 25 4 841.43 0.09 97 21 50 29 713.10 0.75 98 21 54 33 568.39 50.77 99 22 25 3 853.32 3.12 100 22 26 4 848.59 5.47 101 23 26 3 854.25 3.58 102 23 27 4 853.32 3.11 103 23 235 212 110.61 0.29 104 23 236 213 84.98 1.12 105 23 237 214 82.66 0.81 106 23 238 215 80.10 0.24 107 24 27 3 853.32 3.11 108 24 28 4 846.89 6.32 109 24 51 27 624.41 31.53 110 24 54 30 509.12 21.91 111 24 55 31 463.88 2.46 112 25 28 3 851.84 3.83 113 25 29 4 839.99 1.18 114 26 29 3 857.02 5.91 115 26 238 212 76.31 0.60 116 26 241 215 59.55 0.31 117 27 30 3 879.36 6.32 118 27 41 14 806.70 0.57 119 27 55 28 463.87 2.46 120 27 59 32 461.72 2.71 121 27 235 208 90.25 1.26 122 27 237 210 80.93 0.26 123 27 238 211 71.73 1.47 124 27 240 213 59.02 0.40 125 27 241 214 55.26 0.76 126 28 54 26 509.09 21.69 127 28 55 27 463.87 2.46 128 28 56 28 463.79 2.48 129 28 58 30 463.51 2.34 130 28 59 31 462.67 2.29 131 30 58 28 462.50 2.31 132 30 241 211 55.47 0.90 133 30 244 214 48.36 0.04 134 30 245 215 47.16 0.63 135 30 248 218 43.19 0.36 136 31 58 27 461.59 2.69 137 31 248 217 37.06 0.27 138 32 39 7 807.27 0.46 139 32 58 26 461.41 2.66 140 32 59 27 461.43 2.65 141 32 248 216 29.95 0.59 142 33 36 3 857.84 0.83 143 33 37 4 827.30 6.54 144 34 37 3 848.28 3.95 145 34 58 24 461.26 2.65 146 37 244 207 39.18 0.42 147 37 247 210 24.16 0.41 148 37 248 211 20.50 0.07 149 38 205 167 170.42 1.32 150 39 59 20 461.26 2.64 151 39 60 21 461.19 2.64 152 39 244 205 35.89 0.09 153 40 60 20 461.22 2.62 154 40 61 21 461.17 2.63 155 40 62 22 461.12 2.65 156 40 90 50 197.21 0.17 157 41 51 10 502.10 21.75 158 41 55 14 462.56 2.38 159 41 60 19 461.33 2.64 160 41 61 20 461.18 2.63 161 41 62 21 461.17 2.63 162 42 62 20 461.17 2.63 163 42 63 21 461.10 2.64 164 42 64 22 460.89 2.56 165 42 90 48 197.51 0.20 166 42 91 49 197.50 0.20 167 42 92 50 197.28 0.30 168 42 124 82 197.25 0.26 169 43 47 4 819.89 4.15 170 43 48 5 624.25 31.51 171 43 51 8 514.56 21.18 172 43 62 19 461.33 2.63 173 43 64 21 461.11 2.64 174 44 47 3 845.01 0.25 175 44 48 4 837.48 3.51 176 44 64 20 461.12 2.64 177 45 48 3 853.45 3.66 178 47 50 3 848.77 0.95 179 47 51 4 845.40 0.76 180 48 51 3 847.18 1.62 181 48 52 4 838.89 4.04 182 48 61 13 461.51 2.66 183 48 62 14 461.45 2.68 184 48 63 15 461.41 2.65 185 48 88 40 198.47 0.42 186 49 52 3 851.57 0.45 187 49 53 4 850.63 1.83 188 50 53 3 861.90 3.46 189 50 54 4 852.71 0.80 190 51 54 3 861.57 3.63 191 51 55 4 845.51 0.73 192 52 55 3 864.29 2.27 193 52 56 4 864.29 2.27 194 52 57 5 852.70 0.80 195 52 59 7 759.61 24.30 196 52 60 8 462.66 2.29 197 52 61 9 461.64 2.71 198 55 59 4 826.56 0.09 199 55 61 6 524.21 27.69 200 56 59 3 854.81 3.38 201 59 244 185 41.59 0.40 202 60 89 29 197.26 0.15 203 60 90 30 197.23 0.16 204 61 89 28 197.41 0.19 205 61 90 29 197.22 0.17 206 62 88 26 197.53 0.21 207 62 90 28 197.23 0.16 208 63 78 15 203.38 0.74 209 63 83 20 198.81 0.34 210 63 88 25 198.38 0.26 211 63 90 27 197.59 0.21 212 63 91 28 197.47 0.21 213 63 92 29 197.53 0.19 214 64 92 28 197.52 0.20 215 65 92 27 197.68 0.21 216 65 98 33 197.83 0.27 217 65 112 47 200.51 0.56 218 66 78 12 203.53 0.75 219 66 79 13 203.49 0.72 220 66 80 14 203.22 0.69 221 66 91 25 199.11 0.01 222 66 93 27 199.00 0.02 223 66 112 46 200.60 0.48 224 66 116 50 201.55 0.41 225 66 121 55 201.53 0.40 226 67 77 10 204.16 0.67 227 67 78 11 204.14 0.67 228 67 79 12 203.49 0.73 229 67 112 45 201.06 0.65 230 67 115 48 203.13 0.76 231 68 108 40 201.04 0.65 232 68 111 43 201.59 0.55 233 68 112 44 201.04 0.65 234 68 115 47 202.36 0.49 235 69 74 5 566.42 3.20 236 69 79 10 205.09 1.07 237 70 74 4 899.18 0.90 238 70 79 9 205.11 1.08 239 70 81 11 205.09 1.07 240 71 74 3 951.59 3.03 241 71 79 8 205.12 1.07 242 71 81 10 205.13 1.07 243 71 82 11 205.13 1.07 244 74 77 3 857.15 1.01 245 78 82 4 842.00 3.40 246 79 82 3 847.85 2.44 247 79 83 4 846.93 0.93 248 80 83 3 846.94 0.94 249 80 84 4 847.10 0.84 250 80 115 35 347.11 10.27 251 80 116 36 316.82 3.58 252 80 119 39 348.55 10.28 253 80 121 41 348.91 10.46 254 81 84 3 847.12 0.84 255 81 85 4 845.15 0.14 256 81 119 38 362.60 3.75 257 82 85 3 852.81 3.36 258 82 86 4 845.43 0.00 259 83 86 3 845.15 0.14 260 83 87 4 845.14 0.14 261 83 88 5 550.22 2.31 262 83 121 38 477.24 3.94 263 84 87 3 844.77 0.29 264 84 119 35 477.23 3.94 265 84 120 36 477.24 3.94 266 84 121 37 477.24 3.94 267 88 121 33 538.71 0.18 268 89 121 32 538.71 0.19 269 89 122 33 538.67 0.21 270 90 122 32 538.71 0.19 271 90 124 34 538.65 0.21 272 90 161 71 538.68 0.21 273 91 116 25 538.90 0.19 274 91 121 30 538.85 0.18 275 91 122 31 538.78 0.19 276 91 123 32 538.69 0.21 277 91 124 33 538.69 0.20 278 92 95 3 911.24 2.77 279 92 124 32 538.69 0.21 280 92 126 34 538.68 0.20 281 92 230 138 115.17 0.26 282 93 109 16 538.94 0.20 283 93 112 19 538.96 0.20 284 93 123 30 538.88 0.18 285 93 124 31 538.83 0.18 286 93 125 32 538.71 0.18 287 93 126 33 538.71 0.18 288 94 99 5 754.76 30.32 289 94 104 10 539.00 0.21 290 94 109 15 538.95 0.20 291 94 126 32 538.71 0.18 292 95 98 3 857.51 0.79 293 95 99 4 780.44 31.60 294 95 126 31 538.76 0.20 295 95 165 70 538.66 0.17 296 95 230 135 115.64 0.12 297 96 99 3 847.66 5.25 298 96 100 4 766.34 24.55 299 96 165 69 538.70 0.18 300 96 167 71 536.72 0.47 301 96 170 74 482.97 0.40 302 97 100 3 837.20 3.21 303 97 101 4 766.32 24.55 304 97 102 5 762.18 26.62 305 98 102 4 624.43 36.98 306 98 103 5 622.26 35.90 307 98 104 6 616.44 38.43 308 99 103 4 620.28 36.52 309 99 104 5 539.62 0.04 310 99 106 7 539.65 0.03 311 99 109 10 539.62 0.04 312 99 126 27 538.91 0.18 313 99 127 28 538.89 0.18 314 99 146 47 538.96 0.21 315 104 108 4 829.99 1.90 316 104 109 5 842.82 4.50 317 104 112 8 766.13 26.06 318 105 108 3 845.01 3.40 319 105 109 4 845.01 3.41 320 106 109 3 848.30 1.81 321 106 110 4 845.06 3.43 322 106 146 40 538.96 0.21 323 106 149 43 539.36 0.25 324 107 110 3 856.12 5.57 325 107 111 4 845.10 3.45 326 108 111 3 852.80 3.91 327 108 112 4 845.06 3.45 328 109 112 3 857.28 1.67 329 109 113 4 845.08 3.46 330 109 125 16 539.14 0.29 331 109 150 41 539.62 0.18 332 110 113 3 857.28 1.67 333 110 114 4 845.08 3.46 334 110 149 39 539.62 0.18 335 110 150 40 539.62 0.18 336 110 152 42 541.16 0.06 337 110 153 43 541.22 0.04 338 111 114 3 846.80 2.59 339 111 115 4 845.12 3.42 340 112 115 3 856.31 6.53 341 112 116 4 831.20 1.24 342 113 116 3 853.15 0.70 343 113 117 4 831.20 1.24 344 113 123 10 540.11 0.07 345 113 125 12 539.61 0.18 346 113 153 40 559.99 6.85 347 113 154 41 574.86 0.47 348 113 160 47 540.76 0.10 349 114 117 3 853.66 0.51 350 114 118 4 842.26 4.90 351 114 153 39 543.78 1.25 352 115 118 3 858.34 3.15 353 115 119 4 842.26 4.90 354 116 119 3 862.69 5.32 355 116 120 4 842.26 4.90 356 116 121 5 623.02 23.65 357 117 120 3 856.38 2.16 358 117 121 4 832.60 6.00 359 117 153 36 596.12 2.75 360 117 154 37 595.29 2.33 361 117 155 38 629.72 2.02 362 117 160 43 588.15 5.90 363 122 159 37 662.91 2.91 364 123 159 36 663.03 2.91 365 123 160 37 662.99 2.90 366 123 161 38 663.00 2.90 367 124 161 37 663.00 2.90 368 124 163 39 662.98 2.89 369 124 207 83 295.49 1.19 370 124 230 106 115.14 0.28 371 125 150 25 663.26 2.93 372 125 160 35 663.11 2.91 373 125 161 36 663.04 2.90 374 125 162 37 663.01 2.90 375 125 163 38 663.01 2.90 376 126 163 37 663.01 2.90 377 126 165 39 662.97 2.89 378 126 230 104 115.59 0.13 379 127 143 16 663.76 2.97 380 127 146 19 663.57 2.97 381 127 147 20 663.63 2.94 382 127 150 23 663.56 2.98 383 127 162 35 663.11 2.91 384 127 163 36 663.03 2.90 385 127 164 37 663.02 2.90 386 127 165 38 663.00 2.90 387 127 188 61 305.05 0.47 388 128 143 15 663.77 2.97 389 128 146 18 663.72 2.94 390 128 164 36 663.03 2.90 391 128 165 37 663.01 2.90 392 128 167 39 662.85 2.88 393 129 133 4 825.87 1.58 394 129 134 5 769.82 26.40 395 129 139 10 663.82 2.98 396 129 142 13 663.81 2.98 397 129 143 14 663.79 2.98 398 129 164 35 663.10 2.90 399 129 167 38 662.92 2.92 400 130 133 3 853.63 4.21 401 130 134 4 829.94 2.15 402 130 167 37 662.90 2.92 403 131 134 3 861.30 0.39 404 131 135 4 844.27 8.89 405 131 168 37 662.88 2.93 406 131 174 43 586.25 1.58 407 132 135 3 857.39 2.34 408 132 136 4 843.84 9.10 409 133 136 3 857.39 2.34 410 133 137 4 844.28 8.89 411 133 138 5 685.90 10.42 412 133 142 9 663.89 2.98 413 134 137 3 858.53 1.77 414 134 139 5 663.89 2.98 415 134 164 30 663.21 2.88 416 134 168 34 662.92 2.92 417 134 180 46 306.33 0.54 418 138 141 3 854.71 0.64 419 138 142 4 838.11 7.03 420 139 142 3 859.57 1.25 421 139 143 4 840.75 6.75 422 139 184 45 305.29 0.41 423 140 143 3 858.21 1.93 424 140 144 4 840.36 6.55 425 140 187 47 305.17 0.45 426 141 144 3 858.22 1.93 427 141 145 4 849.21 2.40 428 142 145 3 861.46 0.32 429 142 146 4 840.76 6.38 430 143 146 3 849.10 5.65 431 143 147 4 852.87 0.32 432 143 164 21 663.10 2.89 433 143 188 45 305.08 0.48 434 143 191 48 304.82 0.36 435 144 147 3 857.60 2.65 436 144 148 4 852.87 0.31 437 144 191 47 304.68 0.37 438 145 148 3 855.07 0.38 439 145 149 4 853.31 0.50 440 146 149 3 860.96 0.64 441 146 150 4 860.96 0.64 442 147 150 3 870.12 3.03 443 147 151 4 826.21 1.25 444 147 188 41 305.02 0.46 445 147 191 44 304.67 0.37 446 148 151 3 853.33 0.55 447 148 191 43 304.71 0.40 448 148 195 47 299.75 0.94 449 150 153 3 863.52 0.66 450 150 162 12 663.13 2.90 451 151 154 3 869.41 1.94 452 151 192 41 303.68 0.59 453 151 195 44 299.72 0.95 454 151 196 45 299.69 0.95 455 154 157 3 892.50 4.39 456 154 160 6 663.16 2.85 457 156 159 3 872.32 1.37 458 157 192 35 302.88 0.54 459 157 196 39 298.94 0.58 460 157 200 43 297.93 0.64 461 157 203 46 297.86 0.63 462 157 204 47 297.75 0.58 463 158 161 3 922.75 2.58 464 158 203 45 297.86 0.62 465 158 205 47 297.73 0.57 466 160 204 44 297.65 0.60 467 161 205 44 295.97 0.98 468 161 207 46 295.50 1.20 469 162 188 26 304.72 0.56 470 162 192 30 303.38 0.50 471 162 204 42 297.86 0.61 472 162 205 43 297.24 0.43 473 162 206 44 295.65 1.12 474 162 207 45 295.51 1.20 475 163 207 44 295.50 1.20 476 163 209 46 295.07 1.28 477 163 230 67 115.59 0.13 478 164 184 20 305.08 0.48 479 164 185 21 305.07 0.48 480 164 188 24 305.04 0.48 481 164 207 43 295.68 1.11 482 164 208 44 295.49 1.20 483 164 209 45 295.43 1.17 484 165 170 5 820.57 0.06 485 165 209 44 295.37 1.16 486 166 169 3 853.62 2.56 487 166 170 4 844.25 4.88 488 166 176 10 305.80 0.42 489 166 208 42 295.73 1.14 490 166 209 43 295.50 1.20 491 166 210 44 295.37 1.17 492 167 170 3 854.41 2.96 493 167 172 5 821.20 0.38 494 168 172 4 822.45 1.01 495 168 174 6 556.08 68.39 496 168 176 8 327.82 5.88 497 169 172 3 848.60 0.06 498 169 174 5 355.38 7.89 499 169 175 6 322.53 8.54 500 169 176 7 306.30 0.56 501 169 211 42 295.37 1.17 502 170 209 39 295.47 1.21 503 170 210 40 295.32 1.16 504 176 180 4 821.38 0.74 505 176 208 32 296.97 0.80 506 177 180 3 843.84 0.53 507 177 181 4 822.86 1.48 508 178 181 3 852.54 3.17 509 178 182 4 836.01 5.09 510 178 219 41 168.66 20.25 511 178 220 42 129.57 0.71 512 178 223 45 128.10 1.39 513 179 182 3 852.55 3.17 514 179 183 4 836.01 5.09 515 180 183 3 844.52 0.84 516 180 184 4 844.51 0.84 517 181 184 3 865.16 2.46 518 181 185 4 827.83 1.81 519 181 208 27 297.12 0.85 520 181 220 39 129.56 0.70 521 181 226 45 119.85 0.89 522 182 185 3 853.72 0.60 523 182 186 4 831.57 0.01 524 182 223 41 120.18 0.97 525 182 224 42 120.18 0.97 526 182 225 43 120.02 0.96 527 182 226 44 119.97 0.95 528 183 186 3 854.36 2.10 529 183 187 4 831.58 0.01 530 184 187 3 842.79 3.69 531 184 188 4 839.18 3.79 532 185 188 3 852.56 1.20 533 185 189 4 839.17 3.79 534 185 206 21 297.89 0.61 535 185 207 22 297.89 0.61 536 185 208 23 297.82 0.62 537 185 225 40 119.27 0.76 538 185 226 41 118.81 0.53 539 186 189 3 853.06 0.95 540 186 190 4 838.62 6.27 541 186 225 39 119.34 0.72 542 187 190 3 856.19 0.65 543 187 191 4 851.23 0.04 544 188 191 3 853.55 0.27 545 188 192 4 851.23 0.04 546 188 206 18 297.85 0.59 547 189 192 3 859.73 2.83 548 189 193 4 848.99 1.16 549 189 201 12 298.63 0.70 550 189 204 15 298.27 0.61 551 189 206 17 297.89 0.61 552 189 225 36 119.23 0.75 553 189 227 38 119.20 0.73 554 190 193 3 861.05 2.17 555 190 194 4 834.57 8.37 556 191 194 3 848.72 2.89 557 191 195 4 782.11 31.74 558 192 195 3 845.06 3.90 559 192 196 4 720.87 1.25 560 192 197 5 713.01 1.14 561 192 200 8 338.51 3.23 562 192 201 9 298.63 0.70 563 192 204 12 298.22 0.61 564 193 197 4 353.45 3.21 565 193 198 5 351.83 3.29 566 193 201 8 298.75 0.66 567 196 200 4 815.03 0.62 568 197 200 3 851.96 4.36 569 197 201 4 816.27 0.01 570 198 201 3 847.24 2.00 571 198 202 4 846.96 2.16 572 199 202 3 853.55 1.13 573 199 203 4 844.22 3.53 574 200 203 3 847.27 2.00 575 200 204 4 844.23 3.53 576 201 204 3 860.98 4.84 577 204 227 23 117.05 0.01 578 205 227 22 117.05 0.01 579 206 225 19 118.16 0.40 580 206 226 20 117.86 0.28 581 206 227 21 117.06 0.01 582 207 227 20 117.06 0.01 583 207 228 21 116.95 0.01 584 207 229 22 116.89 0.01 585 207 230 23 116.89 0.01 586 208 212 4 867.19 1.60 587 208 220 12 118.27 0.48 588 208 226 18 117.28 0.01 589 208 228 20 117.06 0.01 590 208 229 21 116.94 0.01 591 208 230 22 116.95 0.01 592 209 230 21 116.23 0.29 593 211 216 5 832.80 3.75 594 212 216 4 834.01 4.35 595 212 217 5 468.74 44.09 596 212 220 8 350.11 0.62 597 212 229 17 117.17 0.04 598 212 231 19 117.04 0.01 599 212 234 22 115.75 0.06 600 212 243 31 55.00 1.01 601 213 216 3 868.35 4.01 602 213 217 4 764.93 30.18 603 213 243 30 55.07 1.04 604 214 217 3 864.60 0.61 605 214 239 25 56.39 1.33 606 214 242 28 55.45 0.94 607 214 243 29 55.12 1.02 608 216 219 3 859.65 2.35 609 216 220 4 842.52 0.03 610 217 220 3 848.77 3.09 611 217 221 4 842.52 0.03 612 217 229 12 119.34 0.71 613 217 243 26 47.36 0.15 614 217 246 29 34.47 0.24 615 218 221 3 854.84 5.67 616 218 222 4 842.98 0.26 617 218 246 28 34.38 0.20 618 219 222 3 861.43 0.86 619 219 223 4 842.96 0.27 620 220 223 3 862.12 0.51 621 220 226 6 317.94 1.78 622 220 229 9 119.89 0.85 623 221 226 5 215.45 46.70 624 221 227 6 215.42 46.70 625 221 228 7 119.95 0.86 626 221 229 8 119.96 0.88 627 221 246 25 33.91 0.41 628 221 247 26 29.24 0.52 629 223 226 3 843.56 0.59 630 229 243 14 42.93 0.17 631 229 246 17 33.94 0.42 632 231 234 3 881.36 0.59 633 231 235 4 844.63 1.38 634 231 240 9 55.55 0.91 635 231 243 12 53.93 0.95 636 232 235 3 864.26 2.46 637 232 236 4 861.57 1.11 638 233 236 3 867.63 1.92 639 234 237 3 874.61 5.40 640 234 240 6 495.70 89.14 641 234 243 9 265.42 22.60 642 235 240 5 314.72 1.35 643 237 240 3 875.99 2.14 644 238 241 3 884.50 0.60 645 239 242 3 883.66 5.74 646 239 243 4 853.82 0.48 647 240 243 3 853.84 0.47 648 240 244 4 852.14 1.31 649 241 244 3 853.83 0.47 650 241 245 4 838.85 5.32 651 242 245 3 832.57 3.77 652 242 246 4 831.85 3.42 653 243 246 3 855.96 9.22 654 244 247 3 873.14 3.46 655 244 248 4 832.02 3.50 656 245 248 3 839.08 7.05