BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 7 162 155 177.34 0.97 2 7 165 158 177.51 1.05 3 9 115 106 434.12 0.48 4 9 116 107 433.86 0.42 5 9 117 108 433.81 0.40 6 9 165 156 182.30 0.41 7 10 115 105 434.12 0.48 8 10 116 106 433.88 0.40 9 10 118 108 433.89 0.41 10 10 120 110 433.81 0.40 11 11 113 102 434.62 0.62 12 11 114 103 434.30 0.47 13 11 115 104 434.12 0.48 14 12 113 101 434.82 0.68 15 12 114 102 434.36 0.49 16 12 121 109 433.90 0.40 17 12 123 111 433.75 0.35 18 12 128 116 433.50 0.41 19 12 132 120 432.99 0.51 20 13 111 98 435.99 0.58 21 13 112 99 435.89 0.56 22 13 132 119 433.00 0.52 23 14 111 97 436.10 0.59 24 14 112 98 435.96 0.57 25 14 132 118 432.99 0.51 26 15 58 43 587.92 1.18 27 15 59 44 587.89 1.20 28 15 60 45 587.85 1.17 29 15 61 46 587.84 1.14 30 15 79 64 587.38 0.94 31 15 80 65 587.06 0.98 32 15 81 66 586.71 1.02 33 15 111 96 436.50 0.45 34 15 112 97 436.08 0.58 35 15 132 117 433.49 0.41 36 15 133 118 433.31 0.48 37 15 134 119 433.31 0.48 38 15 135 120 433.44 0.41 39 16 79 63 587.62 1.04 40 16 80 64 587.26 0.89 41 16 81 65 587.04 0.97 42 16 82 66 586.70 1.01 43 16 83 67 586.69 1.01 44 16 86 70 586.68 1.02 45 16 109 93 438.12 0.74 46 16 110 94 437.40 0.61 47 16 111 95 436.54 0.46 48 17 77 60 587.91 1.08 49 17 78 61 587.76 1.10 50 17 79 62 587.62 1.03 51 17 86 69 586.72 1.02 52 17 109 92 438.12 0.74 53 17 110 93 437.74 0.79 54 17 112 95 436.81 0.48 55 17 188 171 208.31 0.29 56 18 77 59 587.92 1.08 57 18 78 60 587.78 1.09 58 18 86 68 586.64 0.98 59 18 90 72 585.83 0.97 60 18 108 90 438.44 0.64 61 18 109 91 438.29 0.66 62 19 75 56 588.50 1.01 63 19 76 57 588.19 1.04 64 19 77 58 587.94 1.09 65 19 107 88 438.57 0.60 66 19 108 89 438.48 0.62 67 19 144 125 209.01 0.00 68 19 201 182 207.33 0.09 69 20 75 55 588.51 1.01 70 20 76 56 588.32 0.98 71 20 93 73 584.99 0.83 72 20 104 84 490.00 10.65 73 20 107 87 438.57 0.60 74 21 67 46 589.83 0.82 75 21 72 51 589.86 0.80 76 21 74 53 589.59 0.75 77 21 75 54 588.51 1.01 78 21 104 83 490.12 10.71 79 21 105 84 473.75 5.41 80 21 106 85 448.92 1.62 81 21 107 86 439.88 1.25 82 21 144 123 278.64 4.25 83 21 233 212 85.42 0.47 84 22 72 50 589.93 0.80 85 22 73 51 589.91 0.80 86 22 74 52 589.66 0.78 87 22 76 54 589.59 0.74 88 22 93 71 585.56 0.89 89 22 94 72 585.32 0.99 90 22 99 77 549.20 2.53 91 22 102 80 532.72 5.17 92 22 103 81 523.68 0.77 93 22 104 82 490.15 10.70 94 22 105 83 490.00 10.65 95 23 70 47 590.10 0.83 96 23 71 48 590.10 0.83 97 23 72 49 589.95 0.81 98 23 73 50 589.91 0.80 99 23 102 79 539.31 1.88 100 23 103 80 524.09 0.93 101 23 105 82 497.29 7.27 102 24 29 5 782.92 3.23 103 24 30 6 782.58 3.04 104 24 73 49 589.95 0.81 105 24 98 74 582.70 0.33 106 24 101 77 542.50 0.70 107 24 102 78 541.66 0.71 108 24 246 222 47.74 0.83 109 24 247 223 45.26 0.70 110 25 28 3 812.71 2.28 111 25 29 4 787.50 0.95 112 25 101 76 542.62 0.65 113 26 29 3 801.29 2.75 114 26 30 4 782.59 3.04 115 26 73 47 590.12 0.84 116 26 246 220 48.23 0.99 117 27 31 4 785.36 4.41 118 28 31 3 809.49 4.75 119 28 32 4 782.61 3.03 120 28 100 72 582.75 0.30 121 28 101 73 572.48 2.32 122 29 32 3 794.26 2.23 123 29 37 8 619.69 4.60 124 29 40 11 611.99 8.44 125 29 98 69 583.06 0.20 126 29 101 72 560.85 2.57 127 29 247 218 48.06 0.97 128 29 249 220 47.91 0.92 129 30 37 7 710.27 20.79 130 30 246 216 51.70 0.27 131 30 247 217 48.11 0.96 132 30 248 218 47.93 0.91 133 32 37 5 779.05 2.33 134 32 101 69 582.73 0.30 135 33 36 3 809.10 1.33 136 33 37 4 793.88 8.32 137 34 37 3 808.49 1.02 138 34 38 4 791.93 1.96 139 34 249 215 48.18 0.97 140 35 38 3 801.27 4.62 141 35 39 4 786.29 4.78 142 36 39 3 800.88 5.44 143 36 40 4 794.86 2.43 144 37 40 3 808.00 0.15 145 37 41 4 791.88 1.66 146 37 247 210 48.61 0.80 147 37 248 211 48.17 0.97 148 37 249 212 47.62 0.88 149 38 41 3 796.43 1.65 150 38 42 4 794.17 2.80 151 38 249 211 47.58 0.86 152 39 42 3 809.27 1.49 153 39 43 4 795.56 3.48 154 40 43 3 807.74 0.73 155 40 44 4 795.64 3.44 156 40 97 57 581.76 0.79 157 40 98 58 581.46 0.83 158 41 44 3 807.74 0.73 159 41 45 4 795.70 3.41 160 41 240 199 72.22 0.46 161 41 243 202 61.24 1.10 162 41 244 203 49.47 0.85 163 41 247 206 48.02 0.96 164 41 249 208 45.11 0.77 165 42 45 3 807.84 0.12 166 42 46 4 795.70 3.41 167 43 46 3 823.83 3.93 168 43 47 4 795.70 3.41 169 43 96 53 584.27 0.69 170 43 97 54 581.73 0.78 171 44 47 3 813.55 5.69 172 44 48 4 794.38 2.77 173 44 93 49 586.29 1.15 174 44 98 54 575.75 0.12 175 44 243 199 57.07 0.46 176 44 247 203 47.68 0.82 177 45 48 3 816.62 4.15 178 45 49 4 803.48 1.81 179 45 240 195 67.73 0.42 180 46 49 3 821.13 1.90 181 46 50 4 816.01 4.45 182 47 50 3 810.91 0.66 183 47 51 4 806.03 3.10 184 47 92 45 586.32 1.15 185 47 93 46 586.00 1.01 186 48 51 3 802.75 3.33 187 48 52 4 801.07 3.02 188 48 62 14 629.79 6.11 189 48 64 16 629.78 6.04 190 49 52 3 807.06 0.03 191 49 53 4 792.57 1.21 192 50 53 3 798.60 4.21 193 50 54 4 792.57 1.20 194 50 92 42 586.53 1.04 195 51 54 3 810.03 4.97 196 51 55 4 793.72 0.62 197 51 62 11 629.82 6.12 198 51 64 13 630.01 6.02 199 51 76 25 629.93 5.98 200 51 78 27 629.69 6.05 201 51 80 29 629.66 6.04 202 51 89 38 586.62 1.07 203 52 55 3 803.48 2.44 204 52 56 4 793.72 0.62 205 52 62 10 630.93 5.59 206 53 56 3 809.66 0.66 207 53 57 4 788.77 1.84 208 54 57 3 800.11 3.82 209 54 58 4 788.77 1.85 210 54 59 5 717.78 2.10 211 54 60 6 677.02 18.28 212 54 80 26 629.70 6.05 213 55 58 3 800.19 3.79 214 55 60 5 686.70 13.44 215 55 61 6 631.25 5.61 216 55 62 7 641.47 0.51 217 55 138 83 433.49 0.41 218 58 135 77 433.51 0.42 219 59 80 21 629.82 6.00 220 59 81 22 629.79 6.00 221 60 79 19 629.84 5.99 222 60 80 20 629.79 5.99 223 61 78 17 629.90 5.96 224 61 79 18 629.87 5.96 225 61 80 19 629.87 5.96 226 61 134 73 433.59 0.42 227 61 135 74 433.47 0.42 228 61 136 75 433.46 0.42 229 62 77 15 629.95 5.96 230 62 78 16 629.87 5.96 231 62 136 74 433.47 0.42 232 62 138 76 433.44 0.42 233 63 76 13 630.02 5.98 234 63 77 14 629.96 5.96 235 63 136 73 433.47 0.42 236 63 237 174 72.83 0.56 237 64 74 10 630.48 5.89 238 64 75 11 630.27 5.92 239 64 76 12 630.02 5.97 240 64 237 173 73.03 0.64 241 64 238 174 72.06 0.68 242 64 243 179 47.85 0.87 243 65 74 9 630.48 5.89 244 65 75 10 630.43 5.92 245 65 77 12 630.41 5.92 246 65 136 71 433.62 0.44 247 65 142 77 385.03 6.83 248 65 203 138 208.33 0.34 249 65 235 170 76.14 0.18 250 65 236 171 75.24 0.26 251 65 237 172 73.48 0.86 252 65 238 173 72.89 0.59 253 66 73 7 630.84 5.75 254 66 74 8 630.67 5.79 255 66 235 169 76.92 0.00 256 66 236 170 76.42 0.14 257 66 238 172 75.11 0.30 258 66 246 180 44.86 0.89 259 67 72 5 630.88 5.75 260 67 73 6 630.86 5.75 261 67 74 7 630.85 5.75 262 67 75 8 630.83 5.75 263 67 233 166 85.22 0.22 264 67 234 167 83.40 0.06 265 67 235 168 77.50 0.28 266 68 234 166 85.25 0.24 267 69 72 3 857.11 2.01 268 71 105 34 491.74 9.92 269 71 231 160 89.89 0.61 270 71 232 161 89.87 0.60 271 71 233 162 89.64 0.49 272 72 105 33 491.74 9.92 273 72 233 161 87.73 0.23 274 74 98 24 580.49 0.35 275 74 102 28 538.86 2.10 276 74 238 164 75.14 0.32 277 74 243 169 48.18 0.97 278 74 246 172 46.26 0.21 279 75 142 67 319.44 7.03 280 76 93 17 584.88 0.84 281 76 102 26 538.95 2.06 282 77 136 59 433.46 0.42 283 77 203 126 206.21 0.24 284 78 86 8 589.74 2.53 285 79 86 7 638.25 8.17 286 79 112 33 437.18 0.66 287 79 134 55 433.50 0.42 288 79 136 57 433.45 0.42 289 80 85 5 698.93 6.98 290 80 86 6 669.87 7.54 291 80 89 9 669.88 7.55 292 82 85 3 831.81 1.84 293 82 86 4 814.40 3.58 294 83 86 3 815.27 3.15 295 83 87 4 801.60 2.77 296 83 109 26 438.38 0.61 297 84 87 3 806.50 1.24 298 84 88 4 797.49 0.42 299 85 88 3 801.90 2.62 300 85 89 4 797.42 0.39 301 86 89 3 814.81 1.60 302 86 90 4 793.83 2.19 303 86 109 23 438.38 0.61 304 87 90 3 798.71 0.22 305 87 91 4 793.81 2.19 306 88 91 3 808.07 4.94 307 88 92 4 795.19 2.06 308 89 92 3 797.75 0.78 309 89 93 4 795.26 2.06 310 90 93 3 799.94 0.26 311 90 94 4 795.31 2.06 312 90 104 14 489.68 10.81 313 91 94 3 795.31 2.06 314 91 95 4 795.31 2.06 315 92 95 3 801.49 2.18 316 92 96 4 795.30 2.06 317 93 96 3 797.18 2.75 318 93 99 6 732.72 1.97 319 94 99 5 732.73 1.98 320 96 99 3 818.78 5.78 321 96 100 4 820.67 1.33 322 97 100 3 820.68 1.33 323 97 101 4 820.68 1.34 324 98 101 3 820.68 1.34 325 98 102 4 815.28 4.03 326 98 247 149 47.89 0.91 327 99 102 3 808.76 6.00 328 104 107 3 937.68 5.94 329 106 144 38 242.51 6.27 330 106 146 40 231.64 11.23 331 106 196 90 230.33 10.63 332 106 231 125 89.86 0.60 333 106 233 127 89.34 0.48 334 108 144 36 231.33 11.15 335 108 146 38 230.94 11.30 336 108 190 82 230.18 10.92 337 108 194 86 230.04 10.83 338 108 196 88 229.99 10.80 339 108 198 90 229.30 10.48 340 108 199 91 214.72 3.46 341 109 190 81 229.99 11.02 342 109 193 84 230.11 10.87 343 109 194 85 230.10 10.86 344 110 188 78 230.03 11.13 345 110 189 79 230.03 11.13 346 110 190 80 229.91 11.05 347 110 193 83 230.13 10.88 348 111 188 77 230.08 11.16 349 111 189 78 230.03 11.13 350 111 193 82 230.15 10.90 351 112 131 19 433.50 0.41 352 112 132 20 433.46 0.42 353 112 134 22 433.00 0.50 354 112 186 74 230.10 11.19 355 112 187 75 230.11 11.19 356 112 188 76 230.07 11.17 357 113 187 74 230.15 11.18 358 113 188 75 230.12 11.14 359 113 189 76 230.14 11.13 360 113 191 78 230.36 11.04 361 113 198 85 229.77 10.95 362 114 121 7 434.07 0.41 363 114 128 14 433.48 0.41 364 114 131 17 433.04 0.48 365 114 132 18 432.62 0.31 366 115 121 6 434.85 0.70 367 116 119 3 833.99 3.36 368 116 120 4 698.00 15.54 369 116 121 5 579.81 34.40 370 117 157 40 231.12 11.04 371 117 162 45 231.10 11.02 372 117 165 48 230.77 11.07 373 117 166 49 230.72 11.09 374 117 169 52 230.75 11.08 375 118 162 44 231.13 11.04 376 118 163 45 231.11 11.02 377 118 166 48 230.89 11.03 378 121 128 7 676.75 13.85 379 122 125 3 797.24 6.21 380 122 128 6 716.88 6.21 381 123 128 5 736.74 3.71 382 123 129 6 716.88 6.22 383 124 129 5 744.25 7.42 384 125 128 3 815.12 3.12 385 125 129 4 787.51 3.02 386 125 130 5 789.42 3.93 387 126 130 4 801.85 0.62 388 127 130 3 834.64 4.12 389 127 131 4 815.75 2.83 390 128 131 3 825.33 0.53 391 128 132 4 821.73 0.15 392 129 132 3 821.73 0.15 393 130 133 3 821.73 0.16 394 131 134 3 803.14 1.28 395 134 186 52 205.31 0.51 396 136 203 67 200.03 0.40 397 137 184 47 208.08 0.37 398 137 203 66 202.80 0.49 399 137 237 100 73.81 0.39 400 138 237 99 73.81 0.39 401 139 205 66 201.11 0.11 402 139 237 98 73.86 0.42 403 140 204 64 205.08 0.30 404 140 205 65 198.81 0.14 405 140 236 96 75.18 0.31 406 140 237 97 73.98 0.29 407 141 203 62 208.25 0.30 408 141 204 63 206.64 0.21 409 141 205 64 205.03 0.33 410 141 206 65 204.77 0.45 411 141 207 66 194.27 1.95 412 141 210 69 147.41 0.90 413 141 235 94 78.03 0.56 414 141 236 95 74.56 0.48 415 142 201 59 208.77 0.43 416 142 202 60 208.52 0.38 417 142 203 61 208.28 0.33 418 142 210 68 146.41 0.42 419 142 234 92 79.93 0.25 420 142 235 93 76.89 0.01 421 143 200 57 209.81 0.67 422 143 201 58 208.94 0.48 423 143 202 59 208.53 0.39 424 143 210 67 139.52 3.79 425 143 232 89 87.10 1.12 426 143 233 90 84.63 0.51 427 143 234 91 79.64 0.10 428 144 199 55 212.16 1.81 429 144 200 56 209.80 0.67 430 144 201 57 208.91 0.48 431 144 232 88 85.07 0.35 432 144 233 89 83.40 0.09 433 145 199 54 212.16 1.81 434 145 200 55 210.13 0.83 435 145 202 57 209.80 0.68 436 145 214 69 137.47 3.27 437 145 229 84 90.00 0.60 438 145 231 86 89.63 0.52 439 145 232 87 85.05 0.34 440 146 196 50 228.91 9.01 441 146 198 52 215.30 3.31 442 146 199 53 212.16 1.81 443 146 229 83 90.00 0.60 444 146 230 84 89.87 0.60 445 146 231 85 89.86 0.61 446 147 196 49 229.11 9.10 447 147 197 50 229.06 9.08 448 147 198 51 221.77 5.90 449 147 200 53 215.41 3.35 450 147 227 80 99.01 1.70 451 147 228 81 90.23 0.51 452 147 229 82 90.00 0.60 453 148 196 48 229.22 9.06 454 148 197 49 228.83 8.95 455 148 198 50 228.81 8.95 456 148 228 80 90.29 0.47 457 149 157 8 271.83 5.55 458 149 158 9 259.43 0.70 459 149 197 48 229.24 9.05 460 149 198 49 229.22 9.06 461 149 222 73 114.78 1.17 462 149 226 77 99.69 1.86 463 150 155 5 479.08 83.80 464 150 156 6 278.98 2.08 465 150 157 7 278.84 2.08 466 150 225 75 113.18 1.28 467 150 226 76 100.58 1.49 468 150 227 77 100.14 1.68 469 150 228 78 99.50 1.87 470 151 155 4 741.49 7.50 471 151 156 5 280.45 1.35 472 151 158 7 278.93 2.09 473 152 155 3 819.21 1.41 474 152 156 4 720.20 3.14 475 153 156 3 839.91 3.73 476 154 225 71 114.55 1.08 477 155 225 70 113.92 1.34 478 155 226 71 113.64 1.47 479 156 159 3 818.27 2.60 480 156 160 4 802.15 3.31 481 156 226 70 113.19 1.26 482 157 160 3 809.61 0.14 483 157 165 8 322.58 7.66 484 157 168 11 302.75 2.25 485 157 169 12 302.75 2.25 486 157 222 65 114.80 1.17 487 157 226 69 112.49 1.54 488 158 197 39 231.56 10.10 489 158 198 40 231.56 10.10 490 160 164 4 785.80 3.71 491 160 165 5 777.00 0.68 492 160 168 8 752.53 6.49 493 160 226 66 113.22 1.26 494 161 164 3 802.86 0.40 495 161 165 4 794.80 9.55 496 162 165 3 809.95 1.99 497 162 166 4 794.81 9.55 498 163 166 3 810.72 1.61 499 163 167 4 807.21 3.36 500 164 167 3 810.72 1.61 501 164 168 4 794.82 9.54 502 165 168 3 805.26 4.33 503 165 169 4 794.62 3.81 504 166 169 3 799.83 1.67 505 166 170 4 788.34 7.42 506 167 170 3 795.85 4.42 507 167 171 4 789.47 6.86 508 168 171 3 819.75 4.51 509 168 172 4 786.56 6.49 510 168 221 53 114.80 1.17 511 168 222 54 114.79 1.16 512 168 226 58 114.36 1.12 513 169 172 3 792.94 8.88 514 169 173 4 786.67 6.42 515 169 222 53 114.96 1.08 516 170 173 3 805.27 5.19 517 170 174 4 797.79 2.38 518 171 174 3 811.17 2.25 519 171 175 4 797.79 2.39 520 172 175 3 807.95 3.85 521 172 176 4 810.16 3.79 522 172 217 45 127.57 3.85 523 172 220 48 119.72 1.25 524 172 222 50 114.98 1.07 525 172 223 51 114.98 1.07 526 173 176 3 816.69 0.54 527 173 177 4 801.39 0.58 528 174 177 3 817.10 0.33 529 175 178 3 857.59 0.78 530 175 179 4 819.67 1.63 531 175 216 41 134.88 4.62 532 175 217 42 131.25 2.84 533 175 220 45 121.98 1.31 534 176 179 3 814.48 0.23 535 176 180 4 804.07 3.27 536 176 213 37 138.78 3.56 537 176 217 41 131.25 2.85 538 177 180 3 805.45 3.97 539 178 216 38 138.90 3.51 540 179 182 3 803.14 0.18 541 179 183 4 767.55 4.46 542 179 209 30 139.84 3.71 543 179 212 33 139.74 3.71 544 179 213 34 138.96 3.57 545 179 216 37 138.87 3.49 546 180 183 3 819.03 8.18 547 182 204 22 231.38 9.50 548 182 209 27 146.43 0.42 549 183 202 19 232.47 9.53 550 183 203 20 231.94 9.75 551 183 204 21 231.41 9.50 552 183 209 26 139.98 3.64 553 183 213 30 139.10 3.50 554 184 202 18 232.46 9.52 555 184 203 19 231.93 9.73 556 185 200 15 232.75 9.46 557 185 201 16 232.66 9.46 558 185 202 17 232.44 9.51 559 186 200 14 232.80 9.45 560 186 201 15 232.65 9.42 561 186 203 17 232.56 9.42 562 187 198 11 233.43 9.17 563 187 199 12 233.12 9.28 564 188 198 10 233.44 9.13 565 188 199 11 233.30 9.15 566 189 193 4 814.22 0.22 567 189 197 8 233.53 9.11 568 189 198 9 233.35 9.08 569 190 193 3 772.77 2.44 570 190 194 4 667.33 1.98 571 190 196 6 245.30 3.66 572 190 197 7 233.47 9.12 573 190 198 8 233.39 9.09 574 191 197 6 233.49 9.12 575 191 198 7 233.49 9.12 576 196 228 32 94.44 0.20 577 196 229 33 90.20 0.52 578 196 230 34 89.99 0.60 579 196 231 35 89.99 0.60 580 202 210 8 251.32 10.97 581 202 213 11 249.50 11.86 582 202 214 12 178.35 23.32 583 203 210 7 300.49 7.63 584 204 209 5 732.95 5.04 585 204 210 6 553.33 46.16 586 206 209 3 809.94 3.65 587 207 210 3 795.70 1.26 588 207 211 4 794.35 0.59 589 207 234 27 83.98 0.71 590 208 211 3 813.88 1.38 591 208 212 4 787.83 0.72 592 209 212 3 811.50 0.69 593 209 213 4 784.06 0.65 594 210 213 3 818.20 3.08 595 210 214 4 785.37 0.06 596 210 234 24 84.64 0.42 597 211 214 3 811.29 0.79 598 211 215 4 776.22 0.29 599 212 215 3 812.25 1.22 600 212 216 4 788.07 5.83 601 213 216 3 800.18 5.84 602 213 217 4 785.05 5.45 603 214 217 3 808.66 0.10 604 214 218 4 797.22 4.58 605 214 232 18 89.71 0.58 606 215 218 3 809.22 1.42 607 215 219 4 806.38 0.01 608 216 219 3 814.90 0.29 609 217 220 3 806.38 0.01 610 217 223 6 323.16 6.03 611 218 223 5 323.16 6.03 612 218 224 6 312.94 0.67 613 218 229 11 263.75 5.00 614 218 232 14 163.83 33.95 615 220 223 3 808.65 4.33 616 220 224 4 774.83 0.02 617 221 224 3 799.17 1.42 618 221 225 4 763.62 5.58 619 221 226 5 762.20 6.29 620 222 226 4 762.20 6.29 621 222 227 5 762.18 6.29 622 223 226 3 799.19 1.43 623 223 227 4 739.44 5.03 624 223 229 6 762.16 6.29 625 238 242 4 698.39 9.00 626 238 243 5 692.18 6.05 627 239 242 3 794.74 3.91 628 239 243 4 787.52 6.87 629 240 243 3 807.02 0.67 630 240 244 4 787.53 6.86 631 241 244 3 788.38 6.50 632 241 245 4 787.93 6.67 633 242 245 3 793.73 9.55 634 242 246 4 787.93 6.67 635 243 246 3 799.03 6.40 636 243 247 4 778.53 1.96 637 244 247 3 801.13 0.00 638 244 248 4 778.54 1.96 639 244 249 5 651.11 8.93 640 245 248 3 776.76 1.08