BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 17 185 153 353.78 0.81 2 17 186 154 353.78 0.81 3 17 187 155 353.78 0.81 4 17 188 156 353.76 0.82 5 17 216 184 199.21 1.79 6 18 45 20 435.44 1.67 7 18 46 21 435.44 1.67 8 18 48 23 435.44 1.66 9 18 186 153 353.83 0.82 10 18 187 154 353.83 0.83 11 18 188 155 353.83 0.83 12 19 46 20 435.47 1.68 13 19 47 21 435.44 1.66 14 19 48 22 435.44 1.66 15 19 188 154 353.86 0.81 16 19 190 156 353.83 0.82 17 19 213 179 259.60 0.10 18 19 216 182 235.48 0.39 19 20 48 21 435.44 1.66 20 20 57 30 357.64 0.59 21 20 73 42 354.54 0.69 22 20 187 152 354.43 0.70 23 20 188 153 354.05 0.81 24 20 189 154 353.86 0.82 25 20 190 155 353.86 0.82 26 21 32 11 436.15 1.40 27 21 36 15 435.84 1.57 28 21 47 19 435.54 1.69 29 21 48 20 435.49 1.68 30 21 49 21 435.44 1.67 31 21 50 22 435.44 1.67 32 21 174 138 354.13 0.76 33 21 190 154 353.99 0.78 34 21 209 173 283.55 0.12 35 21 213 177 267.99 0.26 36 22 32 10 438.13 0.55 37 22 50 21 435.44 1.67 38 22 52 23 435.42 1.68 39 22 53 24 435.19 1.65 40 22 54 25 385.38 5.19 41 22 57 28 359.47 0.07 42 22 189 152 354.47 0.71 43 22 190 153 354.19 0.74 44 22 191 154 353.99 0.76 45 23 28 5 798.69 1.12 46 23 29 6 763.52 3.75 47 23 32 9 766.48 2.27 48 23 50 20 435.49 1.68 49 23 51 21 435.44 1.67 50 23 52 22 435.42 1.68 51 23 192 154 354.02 0.77 52 24 51 20 435.49 1.67 53 24 52 21 435.43 1.67 54 24 53 22 435.42 1.67 55 25 28 3 862.00 1.06 56 25 29 4 855.90 1.99 57 25 51 19 435.50 1.67 58 26 29 3 857.25 2.67 59 26 30 4 855.89 1.99 60 26 51 18 435.52 1.66 61 27 30 3 863.12 0.85 62 27 31 4 855.89 1.99 63 27 202 160 285.38 0.37 64 28 31 3 876.53 5.88 65 28 32 4 851.14 0.37 66 28 192 149 354.22 0.79 67 28 195 152 351.68 1.70 68 28 202 159 285.30 0.38 69 28 206 163 284.08 0.07 70 29 32 3 858.75 3.01 71 29 33 4 856.19 2.17 72 29 49 13 435.66 1.67 73 29 50 14 435.63 1.67 74 29 51 15 435.47 1.67 75 30 33 3 866.97 3.27 76 30 34 4 852.67 3.93 77 31 34 3 865.10 4.21 78 31 35 4 858.62 0.97 79 31 202 156 285.32 0.38 80 31 203 157 285.19 0.39 81 31 206 160 284.07 0.07 82 32 35 3 870.89 5.63 83 32 36 4 842.32 1.85 84 32 49 10 435.67 1.67 85 32 190 143 354.46 0.71 86 32 191 144 354.31 0.74 87 32 192 145 354.11 0.79 88 32 206 159 284.03 0.08 89 32 209 162 283.73 0.10 90 33 36 3 873.03 6.99 91 33 49 9 435.68 1.67 92 35 210 160 283.57 0.12 93 35 214 164 268.57 0.29 94 36 47 4 435.93 1.70 95 36 210 159 283.55 0.12 96 36 213 162 268.49 0.26 97 37 214 162 268.24 0.39 98 37 217 165 255.99 0.83 99 37 277 225 36.29 0.93 100 47 213 158 267.70 0.34 101 50 53 3 1071.37 0.05 102 50 56 6 823.02 0.24 103 50 57 7 395.24 19.27 104 53 56 3 866.50 2.94 105 53 57 4 853.25 2.08 106 54 57 3 871.88 9.90 107 54 58 4 855.36 3.14 108 54 75 17 354.94 0.68 109 54 89 31 354.57 0.65 110 54 92 34 354.62 0.67 111 54 93 35 354.63 0.67 112 54 189 127 354.94 0.68 113 55 58 3 855.83 2.90 114 55 59 4 855.39 3.12 115 55 96 37 354.96 0.85 116 56 59 3 865.30 1.83 117 56 60 4 859.42 0.14 118 57 60 3 873.72 4.95 119 57 61 4 855.08 2.31 120 57 189 124 354.89 0.71 121 58 61 3 860.59 0.70 122 58 62 4 860.59 0.70 123 58 96 34 355.30 0.75 124 58 138 72 355.51 0.70 125 58 140 74 355.48 0.70 126 59 62 3 882.73 3.95 127 60 63 3 882.49 3.83 128 61 189 120 355.29 0.75 129 62 65 3 860.82 5.12 130 62 138 68 355.69 0.72 131 71 139 64 713.87 0.57 132 71 186 111 542.34 1.21 133 72 139 63 713.88 0.57 134 72 184 108 542.50 1.19 135 72 185 109 542.44 1.22 136 72 186 110 542.37 1.22 137 72 187 111 542.37 1.22 138 73 139 62 713.91 0.58 139 73 140 63 713.87 0.57 140 73 141 64 713.85 0.56 141 73 142 65 713.79 0.55 142 73 185 108 542.46 1.22 143 73 187 110 542.37 1.22 144 73 189 112 542.36 1.22 145 74 140 62 713.91 0.58 146 74 142 64 713.79 0.55 147 74 144 66 713.74 0.56 148 74 182 104 542.69 1.22 149 74 185 107 542.59 1.21 150 74 187 109 542.46 1.22 151 74 188 110 542.36 1.22 152 74 189 111 542.36 1.22 153 75 89 14 714.29 0.55 154 75 93 18 714.10 0.57 155 75 140 61 713.96 0.58 156 75 142 63 713.88 0.57 157 75 143 64 713.84 0.56 158 75 144 65 713.81 0.55 159 75 189 110 542.37 1.22 160 76 89 13 714.35 0.54 161 76 144 64 713.82 0.55 162 76 146 66 713.76 0.56 163 76 177 97 542.70 1.20 164 76 188 108 542.58 1.21 165 76 189 109 542.45 1.21 166 76 190 110 542.38 1.22 167 76 191 111 542.37 1.22 168 76 193 113 542.27 1.20 169 77 90 13 714.29 0.55 170 77 93 16 714.26 0.54 171 77 143 62 713.91 0.57 172 77 144 63 713.88 0.57 173 77 145 64 713.81 0.55 174 77 146 65 713.80 0.55 175 78 89 11 714.44 0.54 176 78 90 12 714.41 0.54 177 78 146 64 713.85 0.56 178 78 148 66 708.94 0.41 179 78 191 109 542.48 1.20 180 78 193 111 542.28 1.20 181 79 90 11 714.45 0.54 182 79 145 62 713.93 0.57 183 79 146 63 713.89 0.57 184 79 147 64 713.82 0.55 185 79 148 65 713.74 0.57 186 79 149 66 713.48 0.69 187 80 83 3 859.71 0.98 188 80 84 4 827.77 4.35 189 80 145 61 714.00 0.57 190 80 147 63 713.85 0.56 191 80 149 65 713.64 0.60 192 80 150 66 698.13 0.79 193 80 151 67 678.94 1.01 194 80 152 68 671.76 1.25 195 81 114 33 713.97 0.57 196 81 115 34 713.94 0.56 197 81 117 36 713.90 0.57 198 81 147 62 713.88 0.56 199 81 149 64 713.85 0.56 200 81 150 65 698.14 0.79 201 82 107 25 714.31 0.58 202 82 114 32 714.03 0.58 203 82 150 64 713.42 0.71 204 83 106 23 714.32 0.59 205 83 107 24 714.31 0.59 206 83 110 27 714.19 0.57 207 83 145 58 714.03 0.56 208 84 103 19 714.49 0.58 209 84 107 23 714.32 0.59 210 85 90 5 714.71 0.56 211 85 91 6 714.73 0.55 212 85 94 9 714.67 0.57 213 85 102 17 714.51 0.58 214 85 103 18 714.49 0.58 215 85 106 21 714.42 0.57 216 86 91 5 804.15 8.09 217 87 90 3 877.94 2.87 218 87 91 4 843.56 0.53 219 87 197 106 511.94 2.64 220 88 91 3 871.84 0.83 221 88 92 4 868.55 2.47 222 88 197 105 501.56 2.56 223 89 92 3 873.23 1.02 224 89 93 4 856.66 5.09 225 89 191 98 542.29 1.24 226 90 93 3 866.44 4.41 227 90 94 4 846.19 1.59 228 91 94 3 847.81 0.98 229 91 95 4 859.91 1.28 230 92 95 3 865.21 1.36 231 92 96 4 859.91 1.28 232 93 96 3 864.82 3.37 233 93 97 4 859.91 1.28 234 93 143 46 713.99 0.56 235 94 97 3 880.36 4.12 236 94 98 4 857.46 0.04 237 94 99 5 784.07 4.06 238 94 100 6 787.38 5.70 239 94 103 9 728.87 5.64 240 95 98 3 888.02 0.93 241 96 137 37 721.60 1.23 242 96 138 38 721.74 1.17 243 97 102 5 827.43 4.39 244 97 125 28 721.04 1.30 245 97 137 36 721.62 1.23 246 97 140 39 721.63 1.22 247 97 143 42 719.24 0.92 248 98 101 3 893.47 2.25 249 98 102 4 833.27 1.47 250 99 102 3 877.96 1.90 251 99 103 4 862.36 2.27 252 100 103 3 875.74 3.01 253 100 104 4 862.36 2.27 254 101 104 3 871.05 2.06 255 101 105 4 849.41 1.05 256 102 105 3 870.75 2.21 257 102 106 4 848.23 1.65 258 102 125 23 741.53 5.92 259 102 143 37 740.88 6.02 260 103 106 3 865.27 2.62 261 103 107 4 849.16 1.18 262 104 107 3 877.34 2.71 263 104 108 4 858.94 0.24 264 105 108 3 876.62 2.35 265 105 109 4 850.94 4.24 266 105 123 18 743.15 6.44 267 105 124 19 743.21 6.46 268 105 125 20 743.21 6.46 269 105 127 22 743.28 6.46 270 106 109 3 876.62 2.35 271 106 110 4 850.94 4.24 272 106 123 17 743.08 6.45 273 106 143 33 742.75 6.62 274 106 145 35 742.27 6.79 275 107 110 3 854.17 2.30 276 107 111 4 850.50 4.05 277 107 112 5 781.16 4.74 278 108 111 3 854.64 1.99 279 108 112 4 814.44 5.82 280 109 123 14 743.15 6.44 281 109 127 18 743.31 6.47 282 110 115 5 783.20 15.88 283 110 121 11 743.17 6.42 284 110 123 13 743.18 6.45 285 110 145 31 742.63 6.70 286 114 150 32 731.52 2.98 287 115 119 4 845.45 3.05 288 115 121 6 743.62 6.52 289 115 147 28 742.54 6.78 290 116 119 3 890.83 9.41 291 116 147 27 742.65 6.75 292 117 147 26 742.53 6.79 293 117 148 27 742.52 6.79 294 117 149 28 742.50 6.80 295 117 150 29 742.38 6.78 296 118 176 54 543.12 1.19 297 119 146 23 743.22 6.52 298 119 147 24 743.19 6.53 299 120 145 21 743.27 6.52 300 120 146 22 743.20 6.53 301 120 176 52 543.01 1.20 302 120 180 56 542.95 1.17 303 121 144 19 743.38 6.52 304 121 145 20 743.30 6.54 305 122 142 16 743.78 6.49 306 122 143 17 743.66 6.46 307 122 144 18 743.27 6.58 308 122 180 54 542.92 1.18 309 122 182 56 542.69 1.23 310 123 142 15 743.79 6.49 311 123 143 16 743.71 6.45 312 124 127 3 904.97 1.07 313 124 141 13 744.14 6.47 314 124 142 14 743.93 6.42 315 125 128 3 859.71 0.04 316 125 140 11 744.19 6.47 317 125 141 12 744.16 6.46 318 125 142 13 744.13 6.46 319 125 143 14 744.13 6.47 320 126 141 11 744.18 6.48 321 137 140 3 889.23 0.96 322 140 189 49 542.36 1.23 323 142 182 40 542.66 1.22 324 142 184 42 542.65 1.22 325 142 185 43 542.59 1.21 326 144 177 33 542.65 1.20 327 144 180 36 542.94 1.18 328 144 181 37 542.89 1.19 329 144 182 38 542.88 1.19 330 144 188 44 542.59 1.21 331 146 173 27 542.93 1.22 332 146 177 31 543.01 1.21 333 148 156 8 543.01 1.09 334 148 173 25 543.02 1.22 335 148 194 46 542.32 1.20 336 149 152 3 904.02 2.18 337 149 156 7 543.01 1.09 338 149 198 49 511.80 2.46 339 152 197 45 541.09 1.67 340 155 197 42 542.31 1.19 341 155 198 43 539.29 0.81 342 155 199 44 531.70 2.70 343 156 165 9 544.05 1.22 344 156 169 13 543.90 1.16 345 156 194 38 542.57 1.23 346 156 198 42 542.25 1.22 347 156 200 44 530.88 2.89 348 157 162 5 803.07 15.46 349 157 165 8 544.06 1.22 350 157 166 9 544.05 1.22 351 157 198 41 542.30 1.22 352 157 199 42 537.26 0.09 353 157 200 43 524.25 0.36 354 157 201 44 524.25 0.36 355 158 161 3 868.38 1.66 356 158 162 4 844.23 2.83 357 158 165 7 545.28 0.73 358 159 162 3 865.66 2.27 359 161 164 3 873.61 0.28 360 161 165 4 827.37 3.29 361 162 166 4 805.33 5.50 362 162 167 5 816.62 0.13 363 162 267 105 82.43 0.34 364 163 166 3 846.64 1.59 365 163 167 4 816.62 0.13 366 163 168 5 816.62 0.13 367 163 169 6 827.68 5.40 368 163 235 72 204.71 1.02 369 163 236 73 204.66 0.99 370 163 266 103 82.69 0.33 371 164 168 4 827.44 5.54 372 164 169 5 838.78 0.14 373 164 237 73 204.65 0.99 374 165 169 4 849.79 4.98 375 165 200 35 515.83 3.83 376 166 169 3 881.56 0.34 377 166 170 4 849.78 4.98 378 166 200 34 500.20 0.26 379 166 204 38 285.29 0.34 380 167 170 3 870.75 0.91 381 167 171 4 866.01 2.34 382 167 204 37 285.24 0.32 383 167 207 40 284.31 0.05 384 167 208 41 284.10 0.07 385 167 234 67 204.71 1.02 386 167 266 99 79.74 0.92 387 167 267 100 79.74 0.92 388 168 171 3 878.12 1.33 389 168 172 4 866.01 2.34 390 168 235 67 204.52 0.94 391 168 237 69 204.53 0.96 392 169 172 3 867.86 1.42 393 169 173 4 850.39 0.15 394 170 173 3 865.94 2.31 395 170 174 4 850.59 0.28 396 170 193 23 542.48 1.21 397 170 194 24 542.43 1.20 398 170 200 30 485.48 0.12 399 170 205 35 284.10 0.07 400 170 208 38 283.86 0.12 401 170 209 39 283.76 0.12 402 171 174 3 865.94 2.31 403 171 175 4 844.53 1.65 404 171 208 37 283.80 0.10 405 171 211 40 283.57 0.11 406 171 212 41 283.44 0.10 407 171 215 44 280.81 0.42 408 171 231 60 205.58 0.91 409 171 235 64 204.41 0.96 410 172 175 3 863.50 1.95 411 172 176 4 840.82 0.18 412 173 176 3 863.53 1.94 413 173 177 4 848.73 4.03 414 173 193 20 542.41 1.21 415 174 177 3 864.05 1.91 416 174 178 4 850.17 4.75 417 174 190 16 542.58 1.21 418 174 193 19 542.41 1.21 419 174 209 35 283.55 0.12 420 174 212 38 268.78 0.35 421 174 213 39 268.76 0.36 422 175 178 3 866.47 2.27 423 175 179 4 840.81 0.06 424 175 212 37 279.54 0.41 425 175 215 40 268.57 0.32 426 175 231 56 205.50 0.90 427 176 179 3 862.78 3.00 428 176 180 4 840.74 0.10 429 177 180 3 885.69 6.58 430 177 181 4 840.74 0.10 431 178 181 3 865.13 2.72 432 178 188 10 542.58 1.21 433 178 212 34 268.77 0.35 434 178 213 35 268.68 0.38 435 178 216 38 243.06 0.39 436 181 185 4 874.67 2.39 437 181 188 7 542.48 1.20 438 182 185 3 890.05 1.15 439 190 193 3 1007.68 2.14 440 190 209 19 283.55 0.12 441 191 209 18 283.56 0.12 442 192 195 3 869.94 5.98 443 192 209 17 283.55 0.12 444 193 205 12 284.08 0.07 445 193 209 16 283.55 0.12 446 194 198 4 490.53 0.84 447 194 199 5 486.88 0.26 448 194 200 6 465.03 1.67 449 195 200 5 468.50 3.33 450 195 202 7 285.38 0.36 451 195 205 10 284.82 0.44 452 196 199 3 866.76 0.56 453 200 204 4 863.86 0.96 454 200 205 5 804.53 10.62 455 201 204 3 871.99 3.10 456 201 205 4 855.15 7.57 457 202 205 3 865.31 4.43 458 202 206 4 851.00 5.49 459 203 207 4 870.80 12.96 460 204 207 3 883.35 6.69 461 204 208 4 877.59 9.57 462 205 208 3 883.35 6.69 463 205 209 4 847.58 2.35 464 206 209 3 862.72 1.67 465 206 210 4 846.48 2.91 466 207 210 3 855.78 1.79 467 207 211 4 846.70 2.75 468 207 234 27 204.66 1.00 469 207 266 59 77.11 0.58 470 207 269 62 63.12 0.06 471 207 271 64 55.30 0.85 472 208 211 3 862.74 4.35 473 208 212 4 851.04 0.57 474 208 234 26 204.65 1.00 475 209 212 3 864.87 3.29 476 209 213 4 841.95 0.10 477 210 213 3 846.26 1.82 478 210 214 4 841.95 0.11 479 210 271 61 55.29 0.85 480 211 214 3 855.27 1.17 481 211 215 4 848.39 0.55 482 211 230 19 205.59 0.91 483 211 231 20 205.53 0.92 484 211 234 23 204.61 0.98 485 211 271 60 55.29 0.85 486 211 274 63 54.77 1.10 487 211 275 64 53.93 1.12 488 212 215 3 865.21 1.80 489 212 216 4 861.73 4.36 490 213 216 3 869.63 0.41 491 213 217 4 861.73 4.36 492 214 217 3 861.75 4.36 493 214 218 4 861.74 4.35 494 214 271 57 55.29 0.85 495 214 272 58 55.29 0.85 496 214 275 61 53.57 1.24 497 214 277 63 44.24 0.52 498 215 218 3 862.89 4.92 499 215 219 4 866.98 2.89 500 215 227 12 205.57 0.91 501 215 230 15 205.59 0.91 502 215 231 16 205.55 0.92 503 215 275 60 53.63 1.27 504 216 219 3 880.32 4.94 505 218 221 3 866.90 1.74 506 218 222 4 819.48 0.01 507 218 227 9 205.62 0.90 508 218 275 57 53.88 1.19 509 218 279 61 26.54 0.78 510 219 227 8 589.21 107.77 511 221 254 33 113.19 1.04 512 221 279 58 28.39 0.12 513 222 226 4 836.48 1.88 514 222 227 5 832.30 0.24 515 222 230 8 822.20 4.98 516 222 250 28 113.89 1.15 517 222 254 32 113.39 1.15 518 222 275 53 54.71 1.07 519 222 279 57 30.39 0.87 520 223 226 3 858.99 0.04 521 223 227 4 843.50 1.64 522 223 250 27 113.92 1.15 523 224 227 3 857.01 1.03 524 224 228 4 862.05 7.93 525 225 228 3 866.28 5.81 526 225 229 4 862.05 7.93 527 225 244 19 117.75 0.33 528 225 247 22 113.98 1.16 529 226 229 3 876.94 0.48 530 226 230 4 862.02 7.94 531 226 247 21 113.92 1.15 532 226 250 24 113.92 1.15 533 226 251 25 113.91 1.15 534 226 254 28 113.60 1.11 535 226 256 30 113.23 1.03 536 227 230 3 855.49 11.21 537 227 231 4 851.74 9.34 538 228 231 3 868.51 4.70 539 228 232 4 862.55 3.93 540 229 232 3 864.73 2.85 541 229 233 4 848.60 3.05 542 229 242 13 120.08 0.76 543 229 243 14 117.75 0.30 544 229 244 15 117.87 0.33 545 229 247 18 114.36 1.28 546 229 261 32 109.31 0.27 547 230 233 3 862.69 0.76 548 230 234 4 842.66 0.09 549 230 256 26 113.14 1.03 550 230 261 31 97.33 1.35 551 230 274 44 54.82 1.06 552 230 275 45 54.89 1.09 553 231 234 3 858.97 1.10 554 231 235 4 842.66 0.09 555 232 235 3 863.53 3.39 556 232 236 4 841.00 0.76 557 232 237 5 803.57 3.23 558 232 239 7 185.33 1.69 559 232 242 10 125.56 2.02 560 233 236 3 830.99 5.71 561 233 238 5 503.97 153.03 562 233 243 10 129.93 0.53 563 233 261 28 98.33 1.62 564 233 262 29 96.36 1.81 565 233 264 31 87.03 0.83 566 234 264 30 84.98 0.18 567 234 266 32 80.03 0.61 568 234 271 37 60.52 1.63 569 234 274 40 55.30 0.89 570 236 264 28 90.03 0.27 571 236 265 29 87.03 0.83 572 236 266 30 84.92 0.18 573 239 242 3 862.96 0.04 574 240 243 3 854.22 0.46 575 240 248 8 809.20 1.35 576 240 262 22 112.28 0.67 577 243 247 4 835.73 0.06 578 243 248 5 839.26 1.89 579 243 251 8 827.85 3.71 580 243 258 15 113.25 1.02 581 243 261 18 112.89 0.90 582 243 262 19 112.70 0.85 583 244 247 3 873.43 1.04 584 244 248 4 847.57 2.25 585 245 248 3 869.88 4.04 586 245 249 4 857.37 7.15 587 246 249 3 867.30 2.19 588 246 250 4 868.05 1.81 589 247 250 3 859.89 2.27 590 247 251 4 858.25 3.08 591 248 251 3 858.66 2.88 592 248 252 4 850.00 7.21 593 248 258 10 113.25 1.02 594 248 262 14 112.93 0.90 595 249 252 3 854.53 4.95 596 249 253 4 849.96 7.23 597 250 253 3 849.94 7.23 598 250 254 4 843.59 4.14 599 251 254 3 855.62 1.31 600 251 255 4 823.02 1.31 601 251 256 5 347.50 106.70 602 251 258 7 126.19 2.85 603 251 261 10 131.41 0.24 604 252 255 3 862.83 2.29 605 252 258 6 371.31 114.31 606 254 278 24 54.14 1.22 607 254 279 25 53.52 1.28 608 255 276 21 55.20 0.97 609 256 261 5 832.89 5.48 610 256 273 17 55.48 0.89 611 256 274 18 55.48 0.89 612 256 276 20 55.25 0.95 613 257 260 3 860.87 1.48 614 257 261 4 856.77 6.46 615 258 261 3 859.98 4.86 616 258 262 4 832.94 5.46 617 259 262 3 866.97 1.36 618 259 263 4 851.43 5.88 619 260 263 3 866.45 1.62 620 260 264 4 832.86 5.45 621 260 273 13 60.27 1.14 622 261 264 3 834.95 4.36 623 261 274 13 55.84 0.78 624 264 269 5 125.16 2.54 625 264 270 6 125.18 2.54 626 264 274 10 59.75 0.87 627 265 268 3 861.46 3.87 628 265 269 4 134.21 1.97 629 269 273 4 861.35 2.93 630 270 273 3 874.98 1.88 631 271 274 3 867.54 5.59 632 271 275 4 850.72 2.38 633 272 275 3 861.58 2.80 634 272 276 4 785.54 16.48 635 272 277 5 774.56 11.03 636 275 279 4 845.98 0.10 637 276 279 3 866.03 0.12