BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 6 49 43 534.76 1.46 2 6 50 44 534.72 1.44 3 6 51 45 534.64 1.47 4 6 52 46 534.64 1.47 5 6 83 77 280.51 77.74 6 6 84 78 282.68 76.66 7 6 226 220 87.34 0.08 8 7 52 45 534.65 1.47 9 7 54 47 534.62 1.48 10 7 81 74 285.11 75.73 11 7 82 75 284.74 75.78 12 7 83 76 283.78 76.16 13 7 84 77 284.28 75.86 14 8 40 32 535.24 1.56 15 8 51 43 534.78 1.45 16 8 52 44 534.71 1.46 17 8 53 45 534.64 1.47 18 8 54 46 534.64 1.47 19 8 84 76 284.28 75.86 20 8 86 78 284.89 75.53 21 9 54 45 534.66 1.46 22 9 56 47 534.09 1.41 23 9 73 64 285.21 75.67 24 9 76 67 285.28 75.70 25 9 77 68 285.25 75.71 26 9 82 73 285.21 75.69 27 9 84 75 285.11 75.64 28 9 85 76 284.95 75.56 29 9 86 77 285.79 75.14 30 10 17 7 568.29 11.83 31 10 53 43 534.79 1.44 32 10 54 44 534.72 1.45 33 10 55 45 534.64 1.47 34 10 56 46 534.63 1.47 35 10 73 63 285.73 75.57 36 10 86 76 286.06 75.02 37 10 87 77 286.05 74.97 38 11 17 6 568.30 11.83 39 11 55 44 534.66 1.47 40 11 56 45 534.65 1.47 41 11 59 48 505.65 4.12 42 11 60 49 496.20 8.84 43 11 61 50 471.79 5.24 44 11 73 62 291.55 72.88 45 11 87 76 290.98 72.56 46 12 17 5 577.58 16.46 47 12 18 6 568.29 11.83 48 12 19 7 568.29 11.83 49 12 32 20 543.62 1.05 50 12 55 43 534.79 1.44 51 13 16 3 884.66 6.34 52 13 17 4 784.02 11.61 53 13 61 48 516.94 1.77 54 13 88 75 294.19 70.95 55 13 173 160 158.94 0.42 56 14 18 4 827.35 1.99 57 14 196 182 157.00 0.48 58 15 18 3 881.45 1.63 59 15 34 19 543.51 1.04 60 15 35 20 543.51 1.04 61 15 196 181 155.96 0.03 62 15 250 235 43.55 0.70 63 15 251 236 43.55 0.70 64 16 34 18 543.50 1.05 65 16 35 19 543.46 1.04 66 16 36 20 543.42 1.03 67 16 88 72 291.40 72.36 68 16 106 90 147.30 0.24 69 16 192 176 154.31 0.85 70 16 193 177 155.72 0.14 71 16 216 200 149.82 1.51 72 16 251 235 43.55 0.70 73 17 32 15 543.76 1.12 74 17 33 16 543.73 1.14 75 17 36 19 543.46 1.05 76 17 37 20 543.46 1.04 77 17 55 38 534.79 1.44 78 18 32 14 543.78 1.14 79 18 33 15 543.75 1.14 80 18 34 16 543.73 1.14 81 18 250 232 43.55 0.70 82 19 31 12 543.90 1.13 83 19 32 13 543.81 1.12 84 19 55 36 535.47 1.54 85 20 26 6 544.18 1.03 86 20 30 10 543.95 1.12 87 20 31 11 543.94 1.12 88 20 32 12 543.91 1.14 89 20 33 13 543.83 1.11 90 22 26 4 826.10 2.72 91 26 30 4 803.88 9.94 92 26 31 5 662.89 48.97 93 27 30 3 852.19 2.64 94 27 31 4 803.89 9.95 95 28 31 3 866.94 0.82 96 29 32 3 866.62 0.97 97 29 51 22 535.75 1.50 98 29 52 23 535.60 1.52 99 29 53 24 535.54 1.51 100 32 37 5 823.72 1.76 101 32 53 21 535.53 1.52 102 32 55 23 535.47 1.50 103 33 37 4 818.25 0.97 104 33 250 217 43.55 0.70 105 34 37 3 843.60 0.07 106 34 38 4 832.15 1.52 107 34 250 216 43.55 0.70 108 35 38 3 847.93 3.00 109 35 39 4 827.40 0.85 110 35 105 70 146.97 0.21 111 35 106 71 147.18 0.25 112 35 243 208 45.32 0.67 113 35 246 211 44.05 0.52 114 35 247 212 44.06 0.54 115 35 250 215 43.55 0.70 116 35 251 216 43.55 0.70 117 36 39 3 843.72 7.30 118 36 40 4 841.52 6.21 119 36 86 50 291.16 72.62 120 36 87 51 291.19 72.53 121 36 88 52 291.12 72.49 122 36 105 69 146.58 0.23 123 37 40 3 841.53 6.21 124 37 41 4 841.51 6.21 125 37 53 16 540.31 1.02 126 38 41 3 851.88 1.04 127 38 42 4 841.54 6.21 128 38 246 208 44.05 0.53 129 38 249 211 43.58 0.69 130 38 250 212 43.55 0.70 131 39 42 3 851.88 1.03 132 39 43 4 837.68 6.06 133 39 86 47 291.17 72.62 134 39 105 66 139.66 2.86 135 39 237 198 73.85 0.02 136 39 243 204 45.29 0.67 137 39 246 207 44.05 0.52 138 40 43 3 837.64 6.08 139 40 44 4 837.58 6.11 140 40 49 9 663.80 43.20 141 40 51 11 541.58 1.35 142 40 53 13 541.01 1.26 143 40 86 46 291.10 72.64 144 41 44 3 844.56 5.42 145 41 45 4 821.99 1.71 146 41 51 10 553.46 3.58 147 42 45 3 837.60 6.09 148 42 46 4 824.28 0.56 149 42 237 195 78.75 2.43 150 42 246 204 44.05 0.52 151 43 46 3 838.97 5.50 152 43 47 4 824.25 0.55 153 43 49 6 781.48 4.49 154 43 218 175 134.08 0.10 155 43 230 187 88.26 0.20 156 43 233 190 85.11 0.35 157 43 234 191 85.09 0.34 158 43 237 194 79.61 2.00 159 44 48 4 773.86 6.58 160 44 49 5 672.15 39.02 161 44 51 7 655.40 47.38 162 45 48 3 817.54 0.14 163 46 233 187 85.11 0.33 164 46 236 190 84.81 0.40 165 46 237 191 84.73 0.39 166 47 229 182 88.23 0.20 167 47 230 183 88.15 0.15 168 47 233 186 85.12 0.34 169 49 84 35 285.19 75.62 170 56 59 3 840.39 5.56 171 56 60 4 804.75 4.17 172 56 72 16 286.92 75.17 173 56 73 17 286.35 75.16 174 56 76 20 286.07 75.31 175 57 76 19 290.91 73.11 176 58 71 13 302.66 69.20 177 58 72 14 302.57 69.25 178 60 71 11 303.41 68.94 179 60 72 12 303.00 69.14 180 60 73 13 303.03 69.12 181 60 138 78 174.17 3.19 182 60 139 79 173.77 3.39 183 61 72 11 303.04 69.12 184 61 73 12 303.03 69.12 185 61 74 13 303.04 69.12 186 61 88 27 300.50 67.95 187 61 89 28 300.24 67.88 188 61 95 34 273.40 53.90 189 61 139 78 173.99 3.27 190 61 140 79 172.59 3.56 191 62 70 8 304.01 68.70 192 62 71 9 303.93 68.73 193 62 72 10 303.57 68.87 194 62 74 12 303.04 69.12 195 62 95 33 270.93 52.47 196 62 139 77 174.15 3.18 197 63 70 7 304.04 68.70 198 63 71 8 304.03 68.71 199 63 139 76 174.16 3.17 200 63 143 80 174.22 3.14 201 64 68 4 491.84 26.80 202 64 69 5 304.19 68.72 203 64 70 6 304.06 68.70 204 65 68 3 845.65 2.01 205 65 69 4 305.15 68.84 206 66 147 81 190.48 4.07 207 70 74 4 843.61 2.39 208 70 75 5 789.54 6.00 209 70 78 8 750.26 3.81 210 70 94 24 287.44 44.20 211 70 95 25 287.24 44.31 212 70 98 28 274.76 38.08 213 71 74 3 861.07 3.49 214 71 75 4 808.28 3.21 215 71 139 68 189.60 4.54 216 72 75 3 859.91 4.07 217 72 76 4 839.78 0.34 218 73 76 3 847.39 2.96 219 73 77 4 840.96 0.25 220 73 85 12 317.78 59.40 221 73 87 14 317.69 59.35 222 73 89 16 316.03 58.67 223 74 77 3 857.12 4.83 224 74 78 4 827.17 1.07 225 74 89 15 316.04 58.67 226 74 95 21 283.92 42.41 227 74 98 24 252.68 26.79 228 74 99 25 179.31 9.86 229 75 78 3 851.08 7.86 230 75 79 4 827.17 1.07 231 76 79 3 847.14 3.08 232 76 80 4 827.16 1.07 233 76 81 5 757.18 7.19 234 76 82 6 562.96 0.92 235 77 80 3 824.45 1.32 236 77 82 5 560.22 0.46 237 77 83 6 560.22 0.46 238 77 84 7 560.21 0.46 239 77 85 8 498.82 31.11 240 77 99 22 178.02 10.52 241 77 219 142 165.92 15.82 242 77 220 143 161.16 14.14 243 77 221 144 157.52 12.42 244 78 98 20 178.78 10.20 245 78 99 21 178.19 10.43 246 80 221 141 161.18 14.16 247 82 221 139 165.19 16.18 248 83 220 137 165.22 16.18 249 83 221 138 165.19 16.18 250 84 218 134 166.44 16.09 251 84 219 135 165.51 16.31 252 84 220 136 165.38 16.26 253 84 226 142 88.84 0.43 254 84 230 146 88.32 0.17 255 85 89 4 834.19 2.46 256 85 218 133 165.79 16.42 257 85 219 134 165.64 16.38 258 86 218 132 166.03 16.52 259 87 105 18 195.28 24.05 260 88 216 128 194.97 24.18 261 89 95 6 820.93 0.95 262 89 99 10 555.50 1.78 263 89 101 12 201.93 21.68 264 89 216 127 195.00 24.19 265 89 217 128 194.99 24.19 266 89 219 130 189.12 26.71 267 90 95 5 829.28 5.12 268 90 101 11 275.79 15.16 269 90 141 51 207.84 17.75 270 90 190 100 200.69 21.30 271 90 214 124 200.41 22.23 272 90 215 125 196.82 23.97 273 90 216 126 195.46 23.96 274 91 96 5 829.26 5.12 275 91 101 10 275.79 15.16 276 91 102 11 208.81 18.34 277 91 104 13 200.56 22.35 278 91 215 124 200.41 22.22 279 91 216 125 196.20 24.26 280 91 217 126 195.90 24.12 281 92 95 3 841.34 0.77 282 92 96 4 829.38 5.08 283 92 101 9 430.24 60.86 284 92 141 49 212.47 15.97 285 93 96 3 841.37 0.78 286 93 97 4 841.10 0.65 287 94 97 3 865.50 0.35 288 94 148 54 213.72 15.63 289 95 98 3 854.78 5.71 290 95 99 4 832.25 3.66 291 95 101 6 799.56 3.32 292 95 141 46 213.06 15.93 293 96 99 3 845.86 5.17 294 96 101 5 809.53 1.68 295 97 222 125 193.96 24.57 296 98 222 124 193.71 24.70 297 99 220 121 195.05 24.21 298 99 222 123 193.62 24.67 299 100 219 119 195.84 24.21 300 100 220 120 195.03 24.20 301 100 222 122 191.83 25.55 302 100 223 123 189.68 26.61 303 100 227 127 89.32 0.39 304 101 217 116 204.22 20.30 305 101 218 117 196.23 24.28 306 101 219 118 195.68 24.12 307 101 227 126 89.31 0.39 308 102 217 115 209.10 17.87 309 102 218 116 196.40 24.21 310 102 227 125 89.29 0.38 311 102 230 128 88.88 0.42 312 102 231 129 88.34 0.15 313 102 234 132 87.91 0.04 314 103 231 128 88.87 0.42 315 103 234 131 87.91 0.04 316 104 215 111 213.66 15.73 317 104 216 112 213.13 15.96 318 104 217 113 213.08 15.97 319 104 234 130 87.72 0.34 320 105 216 111 214.16 15.89 321 105 217 112 213.59 15.73 322 105 218 113 213.59 15.74 323 105 234 129 86.21 0.20 324 105 243 138 45.38 0.69 325 106 192 86 335.61 27.70 326 106 215 109 215.61 15.36 327 106 216 110 214.75 15.77 328 106 247 141 44.30 0.48 329 106 251 145 43.58 0.69 330 107 192 85 335.67 27.69 331 107 214 107 217.94 14.76 332 107 215 108 216.76 14.94 333 108 190 82 340.19 29.21 334 108 191 83 335.67 27.68 335 108 192 84 335.67 27.69 336 108 213 105 219.82 14.98 337 108 214 106 218.02 14.79 338 108 215 107 217.96 14.78 339 108 216 108 217.96 14.78 340 109 190 81 340.62 29.00 341 109 191 82 338.43 28.43 342 109 210 101 244.88 20.36 343 109 212 103 238.47 21.96 344 110 142 32 355.00 26.46 345 110 145 35 355.31 26.41 346 110 146 36 355.34 26.39 347 110 188 78 355.19 26.46 348 110 189 79 354.04 26.32 349 110 190 80 342.59 29.92 350 110 210 100 245.00 20.39 351 110 211 101 244.81 20.39 352 110 212 102 244.72 20.41 353 110 214 104 236.63 22.88 354 111 188 77 355.35 26.40 355 111 189 78 355.11 26.42 356 111 194 83 342.88 29.80 357 111 206 95 315.24 27.91 358 111 207 96 312.89 28.15 359 111 209 98 257.49 23.32 360 111 210 99 245.00 20.39 361 112 122 10 355.88 26.37 362 112 123 11 355.78 26.39 363 112 126 14 355.88 26.37 364 112 186 74 355.67 26.41 365 112 187 75 355.50 26.42 366 112 188 76 355.37 26.39 367 112 194 82 343.01 29.82 368 112 205 93 315.96 28.07 369 112 206 94 315.25 27.91 370 112 207 95 312.82 28.12 371 113 177 64 355.61 26.40 372 113 180 67 355.69 26.41 373 113 186 73 355.70 26.39 374 113 187 74 355.56 26.43 375 113 189 76 355.46 26.43 376 113 194 81 342.98 29.84 377 113 197 84 342.54 29.78 378 113 198 85 342.44 29.80 379 113 204 91 318.27 27.45 380 113 205 92 315.95 28.06 381 114 118 4 883.71 1.57 382 114 120 6 360.29 24.33 383 114 132 18 355.96 26.37 384 114 180 66 355.69 26.41 385 114 184 70 355.91 26.39 386 114 185 71 355.78 26.42 387 114 186 72 355.72 26.41 388 114 204 90 318.44 27.53 389 114 205 91 316.68 28.02 390 114 207 93 315.81 28.08 391 115 118 3 925.23 0.88 392 115 183 68 356.03 26.33 393 115 184 69 356.03 26.33 394 115 203 88 319.16 27.46 395 115 204 89 318.63 27.44 396 115 205 90 318.43 27.53 397 116 202 86 335.57 27.09 398 116 203 87 320.79 26.64 399 116 204 88 319.14 27.46 400 116 205 89 319.05 27.45 401 120 125 5 762.41 4.19 402 120 126 6 766.82 2.15 403 120 207 87 315.99 28.03 404 121 125 4 820.36 7.23 405 121 207 86 315.89 28.05 406 121 208 87 315.70 28.07 407 122 125 3 847.59 4.22 408 122 126 4 831.57 1.62 409 122 207 85 315.65 28.06 410 122 208 86 315.39 27.99 411 123 126 3 846.10 3.47 412 123 127 4 823.99 2.17 413 123 154 31 371.99 27.97 414 123 157 34 372.02 27.95 415 123 211 88 310.90 27.18 416 124 127 3 829.29 0.42 417 124 128 4 828.38 0.04 418 124 153 29 372.33 27.82 419 125 128 3 853.39 3.94 420 125 129 4 834.16 2.02 421 126 129 3 845.66 0.08 422 126 132 6 556.93 3.23 423 126 134 8 372.30 27.73 424 126 157 31 458.94 71.00 425 126 186 60 372.30 27.73 426 127 134 7 372.50 27.79 427 127 153 26 372.50 27.78 428 127 156 29 458.95 70.99 429 127 157 30 372.51 27.78 430 129 132 3 917.86 2.12 431 129 134 5 853.83 0.52 432 130 160 30 707.85 3.36 433 132 184 52 708.61 3.06 434 132 185 53 708.53 3.10 435 132 186 54 708.53 3.10 436 133 160 27 710.62 3.27 437 133 164 31 710.34 3.14 438 133 179 46 708.69 3.08 439 133 184 51 708.63 3.06 440 133 185 52 708.62 3.06 441 133 186 53 708.62 3.06 442 134 157 23 710.63 3.26 443 134 160 26 710.62 3.27 444 134 161 27 710.61 3.27 445 134 168 34 710.21 3.19 446 134 185 51 708.63 3.06 447 134 186 52 708.62 3.06 448 134 188 54 708.53 3.10 449 135 161 26 710.61 3.27 450 135 168 33 710.28 3.16 451 135 185 50 708.67 3.06 452 135 186 51 708.68 3.06 453 135 187 52 708.64 3.06 454 135 188 53 708.53 3.10 455 136 142 6 751.78 11.28 456 136 143 7 710.71 3.23 457 136 169 33 710.22 3.19 458 136 170 34 710.19 3.20 459 136 185 49 708.81 3.02 460 136 187 51 708.67 3.06 461 136 188 52 708.58 3.08 462 137 140 3 844.06 0.56 463 137 143 6 728.66 9.54 464 137 170 33 710.16 3.19 465 137 171 34 709.93 3.31 466 137 172 35 709.89 3.31 467 137 173 36 709.82 3.31 468 138 143 5 786.24 5.53 469 138 165 27 710.62 3.19 470 138 166 28 710.61 3.18 471 138 169 31 710.40 3.16 472 138 170 32 710.38 3.16 473 139 143 4 824.62 1.65 474 140 143 3 832.55 2.10 475 140 144 4 824.62 1.65 476 141 144 3 835.15 3.47 477 141 145 4 830.56 1.32 478 141 190 49 708.70 3.06 479 142 145 3 830.95 1.52 480 142 146 4 830.51 1.35 481 142 188 46 708.70 3.06 482 142 190 48 708.64 3.06 483 143 146 3 844.59 1.11 484 143 147 4 824.71 1.48 485 143 158 15 711.54 3.13 486 143 169 26 710.43 3.15 487 144 147 3 832.47 1.31 488 144 148 4 824.70 1.48 489 145 148 3 845.57 1.25 490 145 149 4 811.17 5.28 491 145 154 9 711.97 3.25 492 145 211 66 245.19 20.41 493 145 212 67 245.12 20.46 494 146 149 3 824.89 2.46 495 146 154 8 711.72 3.12 496 146 157 11 711.65 3.12 497 146 158 12 711.52 3.13 498 146 161 15 711.55 3.13 499 146 188 42 708.67 3.06 500 146 211 65 254.16 24.89 501 147 158 11 711.55 3.12 502 149 154 5 783.58 8.87 503 149 155 6 729.14 5.28 504 150 155 5 802.21 3.01 505 151 154 3 840.84 3.33 506 151 155 4 812.94 1.68 507 152 155 3 834.22 6.61 508 152 156 4 812.95 1.68 509 153 156 3 836.25 3.93 510 153 157 4 824.53 1.77 511 154 157 3 824.92 1.57 512 154 158 4 822.75 2.67 513 154 211 57 270.57 29.69 514 155 158 3 833.12 2.52 515 155 159 4 833.12 2.52 516 156 159 3 833.13 2.52 517 156 160 4 833.12 2.52 518 157 160 3 833.30 2.44 519 157 161 4 830.87 3.66 520 157 188 31 708.63 3.06 521 158 161 3 846.34 6.25 522 158 162 4 837.61 10.61 523 158 169 11 710.51 3.10 524 159 162 3 846.35 6.25 525 159 163 4 816.06 3.76 526 160 163 3 866.63 0.07 527 160 164 4 822.26 0.65 528 161 164 3 835.80 1.24 529 161 165 4 785.77 7.55 530 161 168 7 710.50 3.10 531 161 169 8 710.50 3.10 532 161 188 27 708.62 3.08 533 162 165 3 779.27 6.24 534 164 167 3 836.79 0.73 535 164 168 4 748.68 14.76 536 165 168 3 831.03 3.61 537 165 169 4 753.96 17.41 538 167 179 12 709.64 2.59 539 168 185 17 708.66 3.04 540 170 175 5 779.61 1.78 541 170 176 6 716.09 2.48 542 170 179 9 712.64 3.35 543 170 185 15 708.84 3.01 544 170 187 17 708.75 3.03 545 171 175 4 828.09 5.47 546 172 175 3 841.10 1.03 547 172 176 4 828.09 5.47 548 173 176 3 832.49 3.27 549 173 177 4 832.49 3.27 550 173 189 16 708.75 3.03 551 174 177 3 834.74 2.15 552 174 178 4 834.73 2.15 553 175 178 3 834.74 2.15 554 175 179 4 830.48 2.29 555 176 179 3 830.49 2.29 556 176 180 4 830.47 2.28 557 176 187 11 708.75 3.03 558 177 180 3 830.47 2.28 559 177 181 4 830.47 2.28 560 177 187 10 708.81 3.00 561 177 189 12 708.77 3.01 562 177 197 20 525.21 47.42 563 178 181 3 830.46 2.29 564 178 182 4 826.24 4.38 565 179 183 4 794.91 6.05 566 179 185 6 708.92 3.04 567 180 183 3 838.59 3.79 568 180 185 5 708.92 3.04 569 180 187 7 708.90 3.04 570 180 204 24 320.47 26.78 571 181 201 20 340.56 28.84 572 181 204 23 323.49 25.33 573 188 211 23 245.12 20.45 574 189 193 4 825.55 4.54 575 189 194 5 824.80 4.82 576 189 197 8 804.64 4.99 577 190 193 3 865.62 0.75 578 190 194 4 825.54 4.55 579 190 216 26 218.24 14.70 580 191 194 3 825.56 4.55 581 191 195 4 825.55 4.55 582 191 206 15 314.30 27.46 583 191 210 19 244.89 20.36 584 192 195 3 835.39 0.36 585 192 196 4 825.56 4.55 586 192 216 24 218.09 14.77 587 192 247 55 45.31 0.61 588 192 251 59 43.68 0.64 589 193 196 3 847.23 4.83 590 193 197 4 834.38 0.14 591 194 197 3 845.83 5.86 592 194 198 4 825.56 4.61 593 194 206 12 313.60 27.82 594 195 198 3 848.87 6.73 595 195 199 4 824.91 4.92 596 195 247 52 45.38 0.62 597 195 251 56 44.01 0.55 598 196 199 3 848.87 6.73 599 196 200 4 824.93 4.92 600 196 250 54 44.11 0.56 601 196 251 55 43.57 0.69 602 197 200 3 840.94 2.76 603 197 201 4 824.92 4.93 604 197 204 7 318.46 27.59 605 198 201 3 824.36 5.21 606 198 204 6 317.01 28.03 607 198 205 7 316.41 27.84 608 198 206 8 316.39 27.85 609 199 251 52 44.12 0.57 610 200 250 50 44.93 0.73 611 200 251 51 44.48 0.71 612 200 252 52 44.11 0.56 613 201 204 3 853.03 2.60 614 206 209 3 955.02 0.70 615 206 210 4 896.52 2.21 616 218 230 12 88.56 0.28 617 218 234 16 85.16 0.36 618 220 224 4 767.02 2.07 619 220 227 7 88.58 0.28 620 220 230 10 88.85 0.42 621 221 224 3 822.98 0.06 622 224 227 3 850.10 2.40 623 225 229 4 829.66 2.33 624 226 229 3 829.66 2.33 625 226 230 4 814.58 5.21 626 227 230 3 829.67 2.33 627 227 231 4 814.57 5.22 628 228 231 3 818.50 3.26 629 228 232 4 814.57 5.23 630 229 232 3 828.75 1.86 631 229 233 4 811.58 5.49 632 230 233 3 822.25 0.15 633 230 234 4 811.58 5.49 634 231 234 3 845.88 0.36 635 231 235 4 813.43 0.96 636 232 235 3 824.07 2.71 637 232 236 4 813.47 0.96 638 233 236 3 826.40 1.55 639 233 237 4 819.91 2.25 640 234 237 3 838.06 3.65 641 234 238 4 818.97 1.78 642 234 243 9 663.38 44.02 643 235 238 3 841.59 1.88 644 235 239 4 818.97 1.78 645 236 239 3 841.59 1.88 646 236 240 4 818.97 1.78 647 237 240 3 835.18 1.33 648 237 241 4 813.82 4.35 649 237 242 5 796.86 5.42 650 237 243 6 784.45 0.79 651 238 241 3 822.21 0.16 652 238 243 5 796.85 5.42 653 241 244 3 844.55 7.28 654 241 245 4 822.65 5.03 655 242 245 3 824.02 4.34 656 242 246 4 811.84 0.38 657 243 246 3 838.75 3.46 658 243 247 4 822.21 4.81 659 244 247 3 835.22 0.74 660 244 248 4 822.21 4.82 661 245 248 3 835.07 0.67 662 245 249 4 818.71 6.57 663 246 249 3 842.16 2.88 664 246 250 4 818.69 6.59 665 247 250 3 841.01 2.31 666 247 251 4 808.15 1.49 667 248 252 4 755.59 15.45 668 249 252 3 826.42 3.20