BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 114.00 0.59 2 3 228 225 113.68 0.63 3 3 229 226 113.31 0.66 4 4 191 187 170.28 0.11 5 4 204 200 170.29 0.11 6 4 205 201 170.29 0.11 7 4 206 202 170.28 0.11 8 4 207 203 169.78 0.06 9 4 229 225 114.31 0.71 10 5 36 31 671.97 2.41 11 5 37 32 672.00 2.40 12 5 38 33 672.00 2.39 13 5 207 202 170.31 0.09 14 6 36 30 672.01 2.40 15 6 37 31 672.00 2.40 16 6 38 32 672.01 2.39 17 6 223 217 114.48 0.75 18 6 230 224 114.45 0.75 19 6 249 243 24.36 0.67 20 7 37 30 672.01 2.40 21 7 38 31 672.01 2.39 22 7 40 33 672.00 2.39 23 7 90 83 192.40 1.74 24 7 163 156 183.27 0.20 25 7 207 200 182.87 0.23 26 7 209 202 170.52 0.04 27 7 230 223 114.47 0.75 28 7 231 224 114.47 0.75 29 8 37 29 672.10 2.37 30 8 38 30 672.08 2.37 31 8 39 31 672.03 2.38 32 8 40 32 672.02 2.38 33 8 231 223 114.49 0.75 34 8 242 234 53.35 0.29 35 8 245 237 48.40 0.07 36 8 246 238 43.00 0.56 37 9 40 31 672.02 2.38 38 9 124 115 189.57 1.93 39 9 209 200 183.31 0.43 40 9 231 222 116.72 0.07 41 9 232 223 114.47 0.75 42 9 233 224 114.45 0.76 43 9 242 233 53.36 0.29 44 10 40 30 672.08 2.37 45 10 41 31 672.04 2.37 46 10 42 32 672.04 2.37 47 10 237 227 83.20 0.41 48 10 242 232 53.36 0.30 49 11 42 31 672.04 2.37 50 11 65 54 379.69 41.45 51 11 95 84 197.04 4.48 52 11 232 221 117.59 0.24 53 11 233 222 114.91 0.62 54 11 237 226 91.86 0.64 55 12 15 3 886.49 3.75 56 12 21 9 673.18 2.09 57 12 24 12 672.78 2.29 58 12 25 13 672.74 2.31 59 12 28 16 672.72 2.31 60 12 41 29 672.12 2.37 61 12 42 30 672.07 2.37 62 12 43 31 672.06 2.37 63 12 51 39 576.25 4.22 64 12 65 53 379.94 41.51 65 12 237 225 113.13 0.58 66 12 238 226 86.34 1.11 67 13 44 31 672.06 2.37 68 13 65 52 379.56 41.40 69 13 95 82 200.46 3.62 70 13 97 84 200.50 3.63 71 13 234 221 117.61 0.24 72 15 44 29 672.11 2.37 73 15 238 223 113.97 0.64 74 16 21 5 832.64 2.48 75 16 47 31 671.82 2.24 76 17 20 3 869.35 2.19 77 17 21 4 842.78 4.95 78 17 47 30 671.86 2.26 79 18 21 3 869.35 2.19 80 18 22 4 849.00 1.83 81 18 50 32 633.00 8.63 82 19 22 3 870.82 1.45 83 19 23 4 858.83 3.08 84 20 23 3 860.02 3.66 85 20 24 4 860.00 3.68 86 21 24 3 858.95 4.21 87 21 25 4 855.24 6.05 88 21 47 26 671.45 2.09 89 21 50 29 588.81 6.83 90 21 238 217 107.56 1.89 91 22 25 3 856.48 5.44 92 22 26 4 854.50 6.42 93 22 54 32 383.75 40.77 94 23 26 3 868.98 2.60 95 23 27 4 850.82 3.31 96 24 27 3 854.53 4.46 97 24 28 4 838.38 1.65 98 24 238 214 91.93 0.36 99 24 240 216 81.07 0.45 100 25 28 3 852.52 5.48 101 25 29 4 837.44 1.18 102 25 50 25 572.82 6.06 103 25 51 26 453.59 6.54 104 25 54 29 383.68 40.76 105 25 55 30 382.56 41.09 106 26 29 3 846.82 0.10 107 26 30 4 843.20 1.71 108 26 54 28 383.68 40.76 109 27 30 3 862.09 3.28 110 27 31 4 847.25 4.13 111 27 240 213 77.34 1.57 112 27 243 216 65.92 1.08 113 28 31 3 867.60 0.53 114 28 41 13 672.23 2.33 115 28 55 27 382.46 41.04 116 28 60 32 381.58 41.05 117 28 242 214 65.50 1.29 118 29 54 25 383.40 40.62 119 29 55 26 382.46 41.04 120 29 56 27 382.25 40.95 121 29 57 28 381.68 41.05 122 31 57 26 381.67 41.05 123 31 243 212 52.49 0.71 124 31 247 216 50.34 0.36 125 32 247 215 49.60 0.04 126 33 37 4 889.55 3.04 127 33 39 6 673.37 2.56 128 33 57 24 381.67 41.05 129 33 59 26 381.49 41.07 130 33 60 27 381.50 41.07 131 33 246 213 49.59 0.06 132 34 59 25 381.49 41.07 133 35 59 24 381.47 41.08 134 35 246 211 49.48 0.10 135 35 247 212 47.95 0.12 136 35 249 214 37.91 0.10 137 35 250 215 29.09 1.02 138 37 59 22 380.16 41.50 139 37 246 209 43.22 0.03 140 38 61 23 380.03 41.55 141 38 90 52 192.54 1.78 142 39 59 20 380.19 41.48 143 39 60 21 380.04 41.55 144 39 61 22 380.04 41.55 145 39 246 207 45.56 1.14 146 40 61 21 380.04 41.55 147 40 63 23 380.00 41.54 148 40 90 50 192.55 1.79 149 41 51 10 383.36 40.62 150 41 55 14 381.88 40.98 151 41 60 19 381.19 41.05 152 41 61 20 380.30 41.44 153 41 62 21 380.00 41.54 154 41 63 22 380.00 41.54 155 42 63 21 379.95 41.52 156 42 65 23 379.54 41.53 157 42 92 50 196.38 3.64 158 43 47 4 844.70 0.49 159 43 48 5 693.50 62.87 160 43 63 20 380.17 41.49 161 43 65 22 379.94 41.51 162 44 47 3 875.99 4.04 163 44 48 4 847.00 1.65 164 44 65 21 380.05 41.55 165 45 48 3 867.84 0.45 166 45 64 19 380.22 41.51 167 45 66 21 380.16 41.49 168 45 78 33 209.47 2.25 169 47 50 3 865.31 0.78 170 47 51 4 829.61 2.78 171 48 51 3 871.88 4.07 172 48 52 4 828.61 2.41 173 48 62 14 381.46 41.08 174 48 63 15 381.45 41.09 175 48 83 35 207.85 2.81 176 48 86 38 204.16 1.50 177 48 88 40 199.88 3.64 178 49 52 3 836.14 2.33 179 49 53 4 828.61 2.41 180 49 86 37 204.13 1.48 181 50 54 4 828.62 2.41 182 51 54 3 844.28 2.85 183 51 55 4 823.06 5.19 184 51 62 11 381.48 41.08 185 52 62 10 381.50 41.09 186 52 86 34 203.80 1.66 187 55 60 5 381.64 41.04 188 55 62 7 381.58 41.05 189 57 60 3 851.34 0.44 190 60 242 182 57.67 2.18 191 60 246 186 49.55 0.02 192 61 90 29 194.94 2.98 193 62 88 26 199.30 3.55 194 62 90 28 195.47 3.22 195 63 88 25 199.87 3.65 196 63 90 27 195.87 3.40 197 63 91 28 195.96 3.35 198 64 83 19 205.03 2.58 199 64 88 24 200.19 3.60 200 64 90 26 198.77 3.19 201 64 91 27 196.50 3.58 202 64 92 28 196.59 3.63 203 65 92 27 198.83 3.55 204 66 83 17 209.72 2.40 205 66 91 25 200.49 3.61 206 66 92 26 200.47 3.61 207 66 93 27 200.48 3.60 208 66 98 32 200.68 3.62 209 66 112 46 202.06 3.58 210 67 78 11 209.78 2.39 211 67 79 12 209.77 2.39 212 67 80 13 209.74 2.40 213 67 83 16 209.74 2.40 214 67 91 24 200.86 3.58 215 67 93 26 200.75 3.65 216 67 112 45 202.06 3.58 217 68 74 6 345.05 63.17 218 68 77 9 210.30 2.46 219 68 78 10 210.12 2.50 220 68 79 11 209.79 2.40 221 68 80 12 209.83 2.39 222 68 112 44 202.43 3.73 223 69 80 11 209.86 2.38 224 69 81 12 210.24 2.44 225 69 115 46 206.42 2.68 226 70 74 4 922.12 2.89 227 70 79 9 210.25 2.44 228 71 74 3 1007.85 5.12 229 71 79 8 210.31 2.46 230 71 81 10 210.30 2.46 231 71 82 11 210.31 2.46 232 74 77 3 849.99 2.81 233 78 82 4 848.25 1.28 234 79 82 3 860.14 1.40 235 79 83 4 849.03 1.68 236 80 83 3 877.79 0.43 237 80 84 4 847.95 2.21 238 80 91 11 238.23 0.35 239 80 115 35 398.09 10.31 240 80 116 36 284.04 15.35 241 80 119 39 411.72 6.17 242 80 121 41 400.88 11.59 243 81 84 3 851.22 2.18 244 81 85 4 841.21 1.15 245 82 85 3 862.16 0.40 246 82 86 4 841.19 1.14 247 83 86 3 845.47 3.28 248 83 87 4 841.19 1.14 249 83 88 5 699.27 60.75 250 83 121 38 477.02 0.46 251 84 87 3 867.15 7.57 252 84 88 4 832.11 5.67 253 84 119 35 479.24 1.56 254 84 121 37 477.03 0.46 255 88 121 33 555.26 0.19 256 89 120 31 555.27 0.20 257 89 121 32 555.24 0.18 258 89 122 33 555.24 0.18 259 89 161 72 555.26 0.19 260 90 121 31 555.27 0.20 261 90 122 32 555.24 0.18 262 90 124 34 555.25 0.18 263 90 163 73 555.25 0.18 264 91 116 25 555.38 0.17 265 91 121 30 555.34 0.17 266 91 122 31 555.28 0.19 267 91 123 32 555.25 0.18 268 91 124 33 555.25 0.18 269 92 95 3 930.85 5.93 270 92 124 32 555.26 0.18 271 92 126 34 555.32 0.14 272 92 232 140 119.37 0.30 273 93 112 19 555.46 0.17 274 93 113 20 555.41 0.17 275 93 116 23 555.44 0.18 276 93 123 30 555.36 0.15 277 93 124 31 555.35 0.15 278 93 125 32 555.32 0.14 279 93 126 33 555.33 0.14 280 94 99 5 827.35 1.03 281 94 109 15 555.46 0.17 282 94 126 32 555.33 0.14 283 95 98 3 852.71 0.19 284 95 99 4 850.11 1.49 285 95 126 31 555.33 0.14 286 95 167 72 555.05 0.18 287 96 99 3 850.20 1.47 288 96 100 4 829.60 0.10 289 96 167 71 554.74 0.34 290 96 169 73 549.56 0.21 291 97 100 3 855.88 1.38 292 97 101 4 848.77 0.83 293 98 101 3 855.33 1.04 294 98 102 4 827.23 1.05 295 98 103 5 827.09 1.13 296 98 104 6 563.28 3.69 297 99 104 5 555.63 0.12 298 99 106 7 557.09 0.60 299 99 109 10 555.63 0.13 300 99 126 27 555.47 0.16 301 103 108 5 831.59 4.36 302 104 108 4 829.15 5.58 303 104 109 5 821.01 1.51 304 104 112 8 809.18 4.41 305 105 108 3 840.06 8.59 306 105 109 4 833.96 5.54 307 106 109 3 861.61 1.12 308 106 110 4 832.09 4.11 309 106 146 40 555.61 0.16 310 106 149 43 555.70 0.15 311 107 111 4 843.68 1.57 312 108 111 3 862.78 1.67 313 108 112 4 853.14 3.16 314 109 112 3 853.15 3.16 315 109 113 4 853.13 3.17 316 109 150 41 555.89 0.10 317 110 113 3 856.12 4.60 318 110 114 4 854.15 2.66 319 110 149 39 556.02 0.03 320 110 150 40 555.89 0.10 321 110 153 43 557.06 0.17 322 111 114 3 867.71 4.12 323 111 115 4 851.69 4.21 324 111 153 42 558.82 0.74 325 112 115 3 870.60 2.67 326 112 116 4 851.69 4.21 327 113 116 3 865.53 0.14 328 113 117 4 846.09 1.41 329 113 123 10 556.71 0.31 330 113 125 12 555.78 0.09 331 113 150 37 556.95 0.13 332 113 154 41 560.57 0.20 333 114 117 3 861.30 1.32 334 114 118 4 844.06 2.43 335 114 153 39 558.98 0.70 336 114 154 40 560.13 0.12 337 115 118 3 875.76 4.32 338 115 119 4 851.55 6.20 339 116 119 3 864.72 1.21 340 116 120 4 843.68 10.13 341 116 121 5 617.99 28.51 342 116 123 7 560.20 0.09 343 117 120 3 853.61 6.76 344 117 123 6 560.28 0.07 345 117 154 37 696.47 7.30 346 117 158 41 644.93 33.08 347 117 161 44 567.74 3.62 348 117 162 45 560.29 0.07 349 120 161 41 783.37 5.13 350 121 161 40 783.40 5.13 351 122 160 38 783.50 5.10 352 122 161 39 783.40 5.13 353 122 163 41 783.38 5.14 354 122 207 85 337.68 16.81 355 123 161 38 783.50 5.10 356 123 162 39 783.41 5.13 357 123 163 40 783.38 5.14 358 124 163 39 783.43 5.12 359 124 165 41 783.28 5.12 360 124 209 85 335.48 17.66 361 124 232 108 119.56 0.40 362 125 150 25 783.75 5.06 363 125 163 38 783.49 5.10 364 125 164 39 783.43 5.12 365 125 165 40 783.43 5.12 366 126 165 39 783.44 5.12 367 126 167 41 783.32 5.12 368 126 232 106 119.70 0.41 369 127 143 16 784.16 5.03 370 127 146 19 784.05 5.08 371 127 147 20 784.05 5.07 372 127 150 23 783.99 5.09 373 127 164 37 783.59 5.10 374 127 165 38 783.50 5.11 375 127 166 39 783.44 5.12 376 127 167 40 783.41 5.11 377 127 190 63 371.37 13.42 378 128 143 15 784.20 5.03 379 128 146 18 784.19 5.04 380 128 166 38 783.50 5.11 381 128 167 39 783.43 5.12 382 128 169 41 783.29 5.12 383 129 133 4 827.73 7.82 384 129 134 5 818.75 5.27 385 129 139 10 784.26 5.01 386 129 142 13 784.28 5.03 387 129 143 14 784.24 5.03 388 129 166 37 783.59 5.09 389 129 169 40 783.32 5.12 390 129 170 41 783.30 5.12 391 130 133 3 842.60 0.40 392 130 134 4 832.43 5.87 393 130 169 39 783.32 5.12 394 130 170 40 783.29 5.12 395 131 134 3 852.18 4.00 396 131 135 4 832.43 5.87 397 131 170 39 783.30 5.12 398 131 174 43 735.24 1.73 399 131 176 45 487.06 3.45 400 132 136 4 841.25 1.41 401 133 136 3 868.58 3.08 402 133 137 4 836.73 3.67 403 133 138 5 784.59 5.03 404 133 142 9 784.49 5.04 405 134 137 3 848.32 2.13 406 134 139 5 784.49 5.04 407 134 170 36 783.30 5.12 408 134 182 48 372.26 13.64 409 138 141 3 864.15 7.67 410 138 142 4 842.42 1.14 411 139 142 3 851.39 2.04 412 139 143 4 850.65 1.68 413 140 143 3 867.09 3.64 414 140 144 4 858.07 2.00 415 140 189 49 371.63 13.44 416 141 144 3 883.33 3.44 417 141 145 4 858.07 2.01 418 142 145 3 877.92 0.10 419 142 146 4 860.66 3.31 420 143 146 3 869.00 4.58 421 143 147 4 863.57 1.85 422 143 166 23 783.62 5.09 423 143 189 46 371.49 13.43 424 143 190 47 371.45 13.45 425 144 147 3 869.01 4.58 426 144 148 4 861.02 0.58 427 144 189 45 371.50 13.43 428 144 193 49 371.38 13.42 429 145 148 3 863.60 1.84 430 145 149 4 850.95 4.46 431 146 149 3 868.18 0.45 432 146 150 4 861.04 0.56 433 147 150 3 861.03 0.56 434 147 151 4 851.77 4.07 435 147 159 12 784.26 5.16 436 147 164 17 783.63 5.09 437 147 190 43 371.38 13.43 438 147 193 46 371.35 13.42 439 147 194 47 371.33 13.43 440 148 151 3 851.94 1.66 441 148 152 4 846.07 1.20 442 148 193 45 371.33 13.43 443 149 152 3 858.87 2.62 444 150 153 3 891.81 1.09 445 150 154 4 851.04 1.28 446 151 154 3 855.73 3.43 447 151 159 8 784.41 5.08 448 151 193 42 371.33 13.43 449 151 198 47 341.87 16.23 450 152 193 41 371.38 13.44 451 154 158 4 849.11 2.67 452 154 159 5 849.22 3.42 453 154 162 8 785.53 4.49 454 155 158 3 849.95 3.08 455 155 159 4 860.77 6.10 456 156 159 3 883.68 5.34 457 156 198 42 341.76 16.21 458 156 199 43 341.68 16.25 459 156 201 45 341.63 16.24 460 156 202 46 341.45 16.25 461 157 160 3 894.62 0.13 462 158 161 3 888.61 3.14 463 159 162 3 850.36 3.31 464 159 164 5 855.52 0.71 465 159 194 35 369.23 14.25 466 159 198 39 341.71 16.26 467 159 202 43 340.47 16.68 468 159 206 47 338.57 16.43 469 162 206 44 338.54 16.41 470 163 206 43 338.54 16.41 471 163 207 44 337.64 16.82 472 163 209 46 336.83 16.98 473 164 190 26 371.27 13.45 474 164 206 42 338.67 16.48 475 164 207 43 338.52 16.41 476 164 208 44 337.41 16.83 477 164 209 45 336.83 16.98 478 165 209 44 335.47 17.66 479 165 232 67 119.68 0.42 480 166 186 20 371.41 13.43 481 166 187 21 371.36 13.42 482 166 190 24 371.35 13.42 483 166 208 42 337.75 16.78 484 166 209 43 337.41 16.84 485 166 210 44 335.45 17.66 486 166 211 45 335.44 17.66 487 167 211 44 334.69 17.66 488 168 171 3 866.69 3.24 489 168 172 4 810.75 3.81 490 168 178 10 372.01 13.61 491 168 210 42 337.37 16.72 492 168 211 43 335.44 17.64 493 168 212 44 334.44 17.55 494 169 172 3 859.82 7.46 495 170 174 4 753.56 27.60 496 170 176 6 404.48 19.54 497 170 177 7 404.48 19.54 498 170 178 8 395.38 24.03 499 170 182 12 372.18 13.68 500 171 176 5 394.40 24.55 501 171 178 7 372.31 13.63 502 172 211 39 335.42 17.64 503 172 212 40 335.38 17.64 504 178 182 4 846.83 0.22 505 178 210 32 338.54 16.43 506 179 182 3 846.85 0.22 507 179 183 4 846.86 0.23 508 180 183 3 853.38 2.95 509 180 184 4 846.84 0.23 510 180 221 41 174.57 24.31 511 180 222 42 174.50 24.28 512 180 225 45 173.95 24.43 513 181 184 3 856.88 1.69 514 181 185 4 850.40 1.55 515 182 185 3 870.11 0.73 516 182 186 4 853.40 0.05 517 183 186 3 858.84 2.77 518 183 187 4 849.30 0.28 519 183 210 27 338.57 16.45 520 183 222 39 174.46 24.28 521 183 228 45 122.34 0.19 522 184 187 3 850.20 0.46 523 184 188 4 850.71 0.41 524 184 225 41 122.48 0.21 525 184 226 42 122.44 0.18 526 184 227 43 122.31 0.21 527 185 188 3 855.06 1.77 528 185 189 4 850.73 0.41 529 186 189 3 859.42 0.41 530 186 190 4 852.17 1.13 531 187 190 3 857.45 1.40 532 187 191 4 852.16 1.14 533 187 208 21 338.82 16.52 534 187 209 22 338.84 16.52 535 187 210 23 338.63 16.46 536 187 227 40 122.06 0.17 537 187 228 41 122.05 0.18 538 188 191 3 852.12 1.17 539 188 192 4 850.68 0.48 540 188 227 39 122.06 0.17 541 189 192 3 859.86 2.71 542 189 193 4 852.92 0.66 543 190 193 3 867.03 1.53 544 190 194 4 850.88 1.68 545 190 206 16 340.22 16.57 546 190 208 18 338.82 16.53 547 191 194 3 868.40 1.41 548 191 195 4 836.74 2.62 549 191 203 12 341.45 16.36 550 191 204 13 341.44 16.36 551 191 206 15 340.22 16.57 552 191 207 16 340.22 16.57 553 191 208 17 339.23 16.73 554 191 227 36 121.85 0.18 555 191 229 38 121.85 0.18 556 192 195 3 868.43 1.42 557 192 196 4 836.83 2.66 558 193 196 3 839.38 4.58 559 193 197 4 831.55 0.68 560 193 198 5 826.32 3.29 561 194 197 3 847.75 2.04 562 194 198 4 812.45 0.89 563 194 199 5 812.45 0.89 564 194 202 8 404.63 22.50 565 194 203 9 341.70 16.30 566 194 206 12 340.22 16.57 567 195 199 4 795.97 7.33 568 195 200 5 677.91 51.68 569 195 203 8 341.70 16.30 570 198 202 4 856.49 2.59 571 199 202 3 876.38 1.44 572 199 203 4 856.48 2.59 573 200 203 3 856.46 2.60 574 200 204 4 856.46 2.60 575 201 204 3 886.20 0.84 576 201 205 4 857.99 1.83 577 202 205 3 857.99 1.83 578 202 206 4 850.14 0.56 579 203 206 3 857.99 1.82 580 207 229 22 121.39 0.23 581 208 227 19 121.80 0.20 582 208 228 20 121.72 0.18 583 208 229 21 121.62 0.21 584 209 229 20 121.63 0.21 585 209 230 21 121.05 0.34 586 209 231 22 120.06 0.52 587 209 232 23 119.81 0.41 588 210 214 4 875.50 5.17 589 210 222 12 122.10 0.04 590 210 228 18 121.75 0.18 591 210 230 20 121.69 0.19 592 210 231 21 121.23 0.10 593 210 232 22 120.06 0.52 594 211 232 21 120.56 0.13 595 213 218 5 807.27 0.69 596 214 218 4 816.49 3.92 597 214 219 5 402.12 24.75 598 214 222 8 385.23 16.30 599 214 231 17 121.91 0.14 600 214 233 19 121.67 0.20 601 214 236 22 121.34 0.15 602 214 245 31 51.80 1.06 603 215 218 3 866.88 2.65 604 215 219 4 735.89 24.15 605 215 236 21 121.52 0.24 606 215 241 26 53.31 0.32 607 215 245 30 51.80 1.06 608 216 220 4 874.37 2.19 609 216 241 25 53.18 0.38 610 216 244 28 51.81 1.05 611 218 221 3 865.77 2.11 612 218 222 4 835.12 4.03 613 219 222 3 855.86 2.85 614 219 223 4 821.04 3.00 615 219 231 12 122.23 0.15 616 219 245 26 47.66 0.60 617 219 248 29 36.33 0.74 618 220 223 3 851.60 3.77 619 220 224 4 836.02 5.59 620 220 248 28 35.52 1.10 621 221 224 3 861.77 1.89 622 221 225 4 856.15 2.94 623 222 225 3 884.31 4.33 624 222 228 6 122.44 0.21 625 222 231 9 122.34 0.21 626 223 228 5 122.44 0.21 627 223 229 6 122.42 0.22 628 223 230 7 122.39 0.23 629 223 231 8 122.39 0.22 630 223 248 25 35.48 1.10 631 223 249 26 32.52 1.14 632 223 250 27 33.62 0.58 633 225 228 3 855.19 2.43 634 228 231 3 1020.26 0.73 635 230 249 19 25.25 0.84 636 231 245 14 48.41 0.04 637 231 249 18 34.04 2.14 638 233 236 3 895.58 2.25 639 233 237 4 848.94 0.49 640 233 242 9 53.36 0.30 641 233 245 12 50.34 0.34 642 234 237 3 871.53 0.74 643 235 238 3 882.07 5.17 644 235 239 4 865.00 3.61 645 236 239 3 853.72 1.91 646 236 241 5 391.19 17.90 647 236 242 6 126.42 1.57 648 236 245 9 88.64 17.32 649 237 242 5 296.55 29.43 650 239 242 3 874.65 0.43 651 240 243 3 883.90 4.01 652 241 244 3 866.51 1.79 653 241 245 4 840.60 4.62 654 242 245 3 840.78 4.68 655 242 246 4 840.63 4.60 656 243 246 3 859.66 0.17 657 243 247 4 840.64 4.59 658 244 247 3 857.98 0.68 659 244 248 4 844.00 6.30 660 245 248 3 863.77 3.58 661 245 249 4 863.77 3.58 662 246 249 3 880.95 1.83 663 247 250 3 890.04 2.06