BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 111.26 0.43 2 3 228 225 111.25 0.42 3 3 229 226 110.61 0.30 4 4 204 200 112.67 0.59 5 4 205 201 111.81 0.35 6 4 206 202 111.73 0.30 7 4 207 203 111.63 0.30 8 4 229 225 111.64 0.30 9 5 36 31 555.10 46.97 10 5 37 32 555.10 46.97 11 5 38 33 555.07 46.98 12 5 207 202 111.86 0.37 13 6 36 30 555.15 46.97 14 6 37 31 555.13 46.97 15 6 38 32 555.12 46.98 16 6 223 217 111.73 0.30 17 6 229 223 111.67 0.30 18 6 249 243 21.77 0.59 19 7 37 30 555.15 46.97 20 7 38 31 555.12 46.98 21 7 40 33 555.08 46.96 22 7 163 156 224.87 55.25 23 7 207 200 136.78 11.38 24 7 209 202 121.64 4.65 25 7 229 222 112.34 0.55 26 7 230 223 111.70 0.29 27 7 231 224 111.69 0.29 28 8 37 29 555.18 46.97 29 8 38 30 555.15 46.96 30 8 39 31 555.11 46.97 31 8 40 32 555.12 46.96 32 8 231 223 111.70 0.29 33 8 242 234 56.16 0.78 34 8 246 238 44.98 0.64 35 9 40 31 555.11 46.95 36 9 124 115 227.44 55.05 37 9 231 222 112.08 0.42 38 9 232 223 111.70 0.29 39 9 233 224 111.66 0.29 40 9 242 233 56.18 0.79 41 10 40 30 555.16 46.95 42 10 41 31 555.15 46.95 43 10 42 32 555.13 46.95 44 10 237 227 84.98 0.54 45 10 242 232 56.23 0.82 46 11 65 54 458.62 0.12 47 11 95 84 233.78 57.86 48 11 232 221 113.29 0.56 49 11 233 222 111.88 0.35 50 11 234 223 111.67 0.30 51 11 237 226 89.99 0.35 52 12 15 3 883.27 0.46 53 12 21 9 556.26 46.91 54 12 25 13 555.99 46.90 55 12 28 16 556.06 46.81 56 12 41 29 555.21 46.97 57 12 42 30 555.16 46.95 58 12 43 31 555.14 46.95 59 12 65 53 459.74 0.60 60 12 237 225 109.87 0.08 61 12 238 226 87.80 0.74 62 13 44 31 555.16 46.95 63 13 65 52 458.60 0.14 64 13 95 82 235.05 57.63 65 13 97 84 234.93 57.67 66 15 238 223 111.37 0.43 67 16 21 5 656.71 77.88 68 16 47 31 554.89 46.68 69 17 20 3 850.09 1.92 70 17 21 4 804.37 4.08 71 17 47 30 555.29 46.88 72 18 22 4 843.50 4.79 73 18 50 32 552.79 45.48 74 19 22 3 858.65 2.78 75 19 23 4 851.21 0.95 76 20 23 3 859.23 3.08 77 20 24 4 851.73 6.24 78 21 24 3 861.33 2.04 79 21 25 4 845.46 1.94 80 21 47 26 554.86 46.48 81 21 50 29 532.85 34.93 82 22 25 3 865.22 3.98 83 22 26 4 852.89 5.66 84 22 54 32 462.63 0.66 85 23 26 3 871.76 0.70 86 23 27 4 861.40 1.40 87 24 27 3 854.50 2.06 88 24 28 4 854.50 2.06 89 24 237 213 108.64 0.54 90 24 238 214 89.82 1.51 91 24 239 215 86.56 0.13 92 24 240 216 86.33 0.07 93 25 28 3 858.08 0.28 94 25 29 4 858.07 0.27 95 25 51 26 462.57 0.56 96 25 54 29 462.80 0.81 97 25 55 30 462.53 0.95 98 26 29 3 860.08 0.73 99 26 30 4 858.08 0.28 100 26 54 28 462.88 0.85 101 27 30 3 862.78 3.04 102 27 31 4 848.09 4.72 103 27 240 213 83.11 1.04 104 27 243 216 70.92 2.23 105 28 31 3 872.28 1.71 106 28 55 27 462.62 0.99 107 28 57 29 462.26 1.19 108 28 60 32 462.05 1.27 109 28 242 214 70.92 2.23 110 28 243 215 56.25 0.82 111 29 54 25 462.91 0.86 112 29 55 26 462.62 1.01 113 29 56 27 462.61 1.02 114 29 57 28 462.26 1.19 115 31 57 26 462.40 1.26 116 31 243 212 56.38 0.76 117 33 37 4 851.51 1.04 118 33 39 6 580.85 34.61 119 33 57 24 493.99 17.07 120 33 59 26 485.53 13.05 121 33 60 27 485.53 13.05 122 33 246 213 47.98 0.64 123 35 59 24 511.85 26.25 124 35 246 211 49.55 0.33 125 35 247 212 46.34 0.38 126 35 249 214 41.30 1.41 127 35 250 215 24.21 1.49 128 37 246 209 45.01 0.65 129 37 249 212 29.48 0.73 130 38 59 21 511.72 26.36 131 38 60 22 511.56 26.41 132 38 61 23 511.56 26.41 133 38 90 52 235.22 57.70 134 39 59 20 511.73 26.36 135 39 60 21 511.56 26.41 136 39 61 22 511.56 26.41 137 39 246 207 44.99 0.65 138 40 61 21 511.69 26.37 139 40 63 23 511.55 26.42 140 40 90 50 235.23 57.70 141 41 55 14 512.12 26.21 142 41 60 19 511.86 26.30 143 41 61 20 511.78 26.33 144 41 62 21 511.56 26.43 145 41 63 22 511.56 26.41 146 42 63 21 511.56 26.43 147 42 65 23 511.43 26.46 148 42 92 50 235.25 57.70 149 43 47 4 823.56 13.30 150 43 48 5 608.57 21.80 151 43 63 20 511.78 26.34 152 43 65 22 511.56 26.42 153 44 47 3 854.72 1.82 154 44 48 4 854.27 2.05 155 44 65 21 511.75 26.34 156 45 48 3 871.61 5.62 157 45 64 19 511.80 26.34 158 45 66 21 511.79 26.33 159 45 78 33 245.30 57.16 160 45 83 38 244.61 57.19 161 47 50 3 862.37 6.30 162 47 51 4 843.15 5.29 163 48 51 3 851.95 0.90 164 48 52 4 843.44 0.49 165 48 63 15 511.95 26.27 166 48 83 35 243.44 56.61 167 48 86 38 241.53 55.75 168 49 52 3 857.09 9.02 169 49 53 4 833.44 0.42 170 49 86 37 241.53 55.75 171 50 53 3 846.23 0.85 172 50 54 4 833.72 0.28 173 51 54 3 846.11 0.91 174 51 55 4 833.68 0.26 175 51 62 11 512.21 26.18 176 52 62 10 512.21 26.18 177 52 86 34 240.65 56.08 178 55 60 5 512.36 26.23 179 55 62 7 512.24 26.21 180 57 60 3 858.43 2.00 181 60 246 186 47.09 0.76 182 61 90 29 235.51 57.56 183 62 88 26 236.53 57.52 184 62 90 28 236.01 57.33 185 63 88 25 236.59 57.52 186 63 90 27 235.97 57.34 187 63 91 28 235.97 57.34 188 64 83 19 240.70 55.86 189 64 88 24 236.87 57.46 190 64 90 26 236.47 57.50 191 64 91 27 236.09 57.31 192 64 92 28 236.09 57.30 193 65 92 27 236.18 57.34 194 66 83 17 244.95 57.33 195 66 91 25 236.69 57.47 196 66 92 26 236.61 57.45 197 66 93 27 236.59 57.45 198 66 98 32 236.62 57.47 199 66 112 46 236.83 57.38 200 67 78 11 245.46 57.08 201 67 79 12 245.45 57.09 202 67 80 13 245.38 57.12 203 67 83 16 245.43 57.09 204 67 91 24 236.85 57.41 205 67 112 45 236.88 57.37 206 68 74 6 557.03 3.88 207 68 77 9 245.63 57.09 208 68 78 10 245.59 57.09 209 68 79 11 245.49 57.09 210 68 80 12 245.48 57.09 211 68 112 44 239.42 56.12 212 69 80 11 245.52 57.08 213 69 81 12 245.55 57.06 214 69 115 46 241.24 55.21 215 70 74 4 896.42 3.19 216 70 79 9 245.61 57.09 217 71 74 3 945.86 0.66 218 71 79 8 245.62 57.09 219 74 77 3 874.39 0.58 220 78 82 4 842.16 0.71 221 78 83 5 836.53 2.10 222 79 82 3 852.58 4.49 223 79 83 4 837.03 1.85 224 80 83 3 854.13 3.71 225 80 84 4 837.05 1.85 226 80 91 11 367.07 2.40 227 80 115 35 418.12 27.83 228 80 116 36 418.04 27.87 229 80 119 39 485.75 8.92 230 80 121 41 424.19 30.44 231 81 84 3 848.03 0.66 232 81 85 4 843.74 1.49 233 82 85 3 855.52 2.33 234 82 86 4 838.91 0.93 235 83 86 3 844.16 1.70 236 83 87 4 828.35 4.34 237 83 88 5 808.97 4.77 238 83 91 8 472.76 51.26 239 83 121 38 539.89 25.65 240 84 87 3 833.14 6.74 241 84 88 4 817.77 0.95 242 84 119 35 544.58 27.75 243 84 121 37 539.92 25.61 244 88 121 33 596.44 28.24 245 89 121 32 596.43 28.25 246 89 122 33 596.43 28.25 247 90 121 31 596.46 28.25 248 90 122 32 596.43 28.24 249 90 124 34 596.43 28.24 250 91 116 25 596.60 28.25 251 91 121 30 596.54 28.23 252 91 122 31 596.53 28.23 253 91 123 32 596.47 28.25 254 91 124 33 596.47 28.25 255 92 95 3 918.92 1.57 256 92 124 32 596.47 28.25 257 92 126 34 596.45 28.24 258 92 232 140 163.16 22.66 259 93 112 19 596.69 28.23 260 93 113 20 596.62 28.25 261 93 116 23 596.69 28.23 262 93 123 30 596.55 28.24 263 93 124 31 596.52 28.25 264 93 125 32 596.48 28.25 265 93 126 33 596.47 28.25 266 94 99 5 828.11 4.90 267 94 104 10 596.71 28.23 268 94 109 15 596.67 28.24 269 94 126 32 596.48 28.25 270 95 98 3 855.73 0.61 271 95 99 4 842.37 2.14 272 95 126 31 596.48 28.25 273 95 167 72 596.42 28.25 274 95 232 137 165.45 21.52 275 96 99 3 851.21 1.66 276 96 100 4 842.37 2.14 277 96 167 71 596.39 28.27 278 96 169 73 593.71 28.62 279 97 100 3 863.19 6.63 280 97 101 4 842.36 2.14 281 97 102 5 828.23 4.92 282 98 101 3 863.15 1.69 283 98 102 4 828.11 4.98 284 98 103 5 828.11 4.98 285 98 104 6 681.00 68.19 286 99 103 4 827.95 4.92 287 99 104 5 681.00 68.19 288 99 105 6 681.02 68.19 289 99 106 7 683.12 67.14 290 99 109 10 681.00 68.19 291 99 126 27 596.58 28.25 292 99 146 47 596.76 28.25 293 103 108 5 864.59 10.20 294 104 108 4 845.25 1.53 295 104 109 5 832.80 2.79 296 104 112 8 831.97 2.73 297 105 108 3 875.61 4.70 298 105 109 4 867.45 8.77 299 106 109 3 875.61 4.69 300 106 110 4 846.25 1.03 301 106 145 39 596.85 28.23 302 106 146 40 596.80 28.25 303 106 149 43 596.88 28.24 304 107 110 3 873.29 5.85 305 107 111 4 846.25 1.03 306 108 111 3 858.01 0.69 307 108 112 4 843.34 2.46 308 109 112 3 853.14 1.84 309 109 113 4 842.82 2.71 310 109 150 41 597.08 28.26 311 110 113 3 868.99 1.09 312 110 114 4 842.98 2.79 313 110 149 39 596.95 28.23 314 110 150 40 596.97 28.22 315 110 153 43 597.37 28.23 316 111 114 3 868.98 1.09 317 111 115 4 848.38 2.90 318 111 153 42 597.37 28.24 319 112 115 3 856.00 3.27 320 112 116 4 845.59 1.49 321 113 116 3 849.32 0.37 322 113 117 4 835.22 3.71 323 113 123 10 597.22 28.24 324 113 125 12 596.98 28.19 325 113 150 37 597.28 28.21 326 113 154 41 597.50 28.25 327 114 117 3 849.30 0.73 328 114 118 4 843.31 0.34 329 114 153 39 597.45 28.25 330 114 154 40 597.48 28.24 331 115 118 3 849.39 0.69 332 115 119 4 847.38 1.69 333 116 119 3 849.31 0.73 334 116 120 4 849.30 0.73 335 116 121 5 671.15 8.61 336 116 123 7 597.44 28.24 337 117 120 3 868.86 1.12 338 117 123 6 723.50 34.61 339 117 154 37 672.74 2.24 340 117 158 41 673.99 10.21 341 117 162 45 597.47 28.24 342 120 161 41 783.32 1.56 343 121 161 40 783.34 1.56 344 122 160 38 783.39 1.57 345 122 161 39 783.34 1.56 346 123 161 38 783.38 1.56 347 123 162 39 783.35 1.56 348 123 163 40 783.34 1.56 349 124 163 39 783.34 1.56 350 124 165 41 783.28 1.58 351 124 209 85 244.80 25.53 352 125 162 37 783.45 1.55 353 125 163 38 783.38 1.57 354 125 164 39 783.35 1.56 355 125 165 40 783.33 1.57 356 126 165 39 783.35 1.57 357 126 167 41 783.31 1.57 358 126 232 106 165.44 21.52 359 127 143 16 783.78 1.60 360 127 146 19 783.69 1.57 361 127 147 20 783.68 1.57 362 127 164 37 783.45 1.55 363 127 165 38 783.40 1.56 364 127 166 39 783.34 1.57 365 127 167 40 783.32 1.58 366 127 190 63 279.33 15.65 367 128 143 15 783.87 1.60 368 128 146 18 783.87 1.60 369 128 166 38 783.37 1.56 370 128 167 39 783.34 1.56 371 128 169 41 783.02 1.45 372 129 133 4 834.80 1.90 373 129 134 5 829.73 4.50 374 129 139 10 784.01 1.60 375 129 142 13 784.00 1.60 376 129 143 14 783.91 1.60 377 129 166 37 783.45 1.55 378 129 167 38 783.38 1.56 379 129 169 40 783.02 1.46 380 129 170 41 783.00 1.46 381 130 133 3 857.02 0.28 382 130 134 4 839.45 0.33 383 130 169 39 783.23 1.54 384 130 170 40 783.00 1.47 385 131 134 3 857.02 0.27 386 131 135 4 839.46 0.32 387 131 170 39 783.03 1.45 388 131 174 43 740.03 0.42 389 131 176 45 706.07 2.11 390 132 135 3 853.35 1.77 391 132 136 4 839.45 0.32 392 132 138 6 820.82 8.87 393 133 136 3 854.84 1.02 394 133 137 4 834.92 1.94 395 133 138 5 796.80 4.09 396 133 139 6 796.77 4.08 397 133 142 9 784.45 1.48 398 134 137 3 841.56 1.38 399 134 139 5 785.05 1.78 400 134 170 36 783.08 1.43 401 134 182 48 280.67 15.78 402 138 141 3 861.20 4.08 403 138 142 4 843.86 2.30 404 139 142 3 852.45 3.42 405 139 143 4 843.85 2.30 406 140 143 3 864.72 2.31 407 140 144 4 837.37 0.96 408 140 189 49 279.39 15.66 409 141 145 4 837.41 0.96 410 142 145 3 853.47 0.34 411 142 146 4 839.50 0.07 412 143 146 3 846.90 3.62 413 143 147 4 839.30 0.18 414 143 166 23 783.44 1.55 415 143 190 47 279.35 15.64 416 144 147 3 853.71 7.02 417 144 148 4 845.97 3.16 418 144 193 49 279.16 15.74 419 145 148 3 845.98 3.15 420 145 149 4 845.98 3.16 421 146 149 3 858.59 1.79 422 146 150 4 857.22 2.47 423 147 150 3 857.23 2.47 424 147 151 4 851.85 1.57 425 147 159 12 784.04 1.34 426 147 164 17 783.46 1.56 427 147 190 43 279.34 15.64 428 147 193 46 279.13 15.75 429 147 194 47 279.03 15.75 430 148 151 3 858.57 1.79 431 148 152 4 851.84 1.56 432 148 193 45 279.31 15.66 433 149 152 3 871.36 1.36 434 149 153 4 862.56 3.38 435 150 153 3 875.52 1.19 436 150 154 4 862.15 3.58 437 151 154 3 866.41 5.74 438 151 159 8 784.31 1.23 439 151 193 42 279.11 15.73 440 151 198 47 258.54 19.74 441 154 158 4 825.98 1.82 442 154 159 5 813.29 4.12 443 154 162 8 784.20 1.28 444 155 158 3 862.38 3.85 445 155 159 4 861.68 3.02 446 156 159 3 868.91 0.59 447 156 201 45 246.86 25.59 448 156 202 46 246.59 25.64 449 157 160 3 883.54 0.38 450 158 161 3 867.14 1.72 451 158 162 4 859.00 4.37 452 159 162 3 854.40 2.07 453 159 198 39 246.91 25.59 454 159 202 43 246.29 25.64 455 159 206 47 245.58 25.57 456 162 206 44 245.55 25.59 457 163 207 44 245.40 25.61 458 163 209 46 245.11 25.49 459 164 190 26 279.03 15.75 460 164 206 42 245.84 25.69 461 164 207 43 245.66 25.64 462 164 208 44 245.11 25.48 463 164 209 45 245.09 25.48 464 165 209 44 244.80 25.53 465 165 232 67 165.45 21.52 466 166 186 20 279.33 15.64 467 166 187 21 279.32 15.64 468 166 190 24 279.32 15.65 469 166 208 42 245.59 25.69 470 166 209 43 245.34 25.64 471 166 210 44 244.76 25.52 472 166 211 45 244.56 25.52 473 167 172 5 846.87 3.04 474 168 171 3 885.20 3.46 475 168 172 4 869.09 0.96 476 168 210 42 245.33 25.81 477 168 211 43 244.83 25.59 478 168 212 44 244.31 25.57 479 169 172 3 869.08 0.96 480 169 174 5 850.81 8.17 481 170 174 4 847.65 9.74 482 170 176 6 682.76 6.80 483 170 178 8 314.78 24.20 484 170 182 12 280.51 15.86 485 171 174 3 845.77 3.57 486 171 176 5 375.40 6.08 487 171 177 6 375.38 6.09 488 171 178 7 289.28 11.48 489 172 211 39 244.74 25.63 490 172 212 40 244.14 25.65 491 177 182 5 854.99 2.19 492 178 182 4 842.07 0.37 493 178 210 32 245.74 25.77 494 179 182 3 863.41 1.48 495 179 183 4 851.08 4.13 496 180 183 3 855.00 2.19 497 180 184 4 847.49 3.23 498 180 221 41 173.87 24.62 499 180 222 42 173.87 24.62 500 180 225 45 168.21 22.00 501 181 184 3 848.38 2.79 502 181 185 4 847.48 3.23 503 181 225 44 168.19 22.02 504 182 185 3 847.49 3.23 505 182 186 4 847.76 3.08 506 183 186 3 867.58 4.62 507 183 187 4 857.44 1.77 508 183 210 27 245.94 25.70 509 183 222 39 173.76 24.57 510 184 187 3 869.76 4.54 511 184 188 4 856.61 2.19 512 184 225 41 167.52 22.08 513 184 226 42 167.51 22.08 514 184 227 43 167.37 22.05 515 185 188 3 863.61 1.46 516 185 189 4 857.20 2.48 517 186 189 3 870.24 4.03 518 186 190 4 857.20 2.48 519 187 190 3 866.27 2.05 520 187 191 4 842.02 0.24 521 187 208 21 246.00 25.71 522 187 209 22 246.00 25.71 523 187 210 23 245.97 25.71 524 187 227 40 166.80 21.83 525 187 228 41 166.78 21.84 526 188 191 3 855.47 0.15 527 188 192 4 842.05 0.26 528 188 227 39 166.81 21.83 529 189 192 3 865.45 2.51 530 189 193 4 846.58 2.53 531 190 193 3 865.03 2.69 532 190 194 4 842.05 0.29 533 190 208 18 246.02 25.69 534 191 194 3 858.98 3.39 535 191 195 4 838.33 2.16 536 191 203 12 247.25 25.57 537 191 204 13 247.25 25.57 538 191 206 15 246.77 25.75 539 191 208 17 246.15 25.64 540 191 227 36 166.78 21.84 541 191 229 38 166.77 21.84 542 192 195 3 841.96 2.52 543 192 196 4 838.30 2.16 544 193 196 3 861.84 2.60 545 193 197 4 816.42 8.14 546 193 198 5 816.42 8.15 547 194 197 3 850.86 1.93 548 194 198 4 816.39 8.14 549 194 199 5 816.39 8.14 550 194 202 8 298.90 20.52 551 194 203 9 253.66 28.77 552 194 206 12 246.97 25.66 553 195 199 4 814.31 8.37 554 195 200 5 290.70 15.39 555 195 203 8 247.31 25.55 556 198 202 4 827.40 3.99 557 199 202 3 840.43 6.44 558 199 203 4 831.20 2.10 559 200 203 3 833.09 1.15 560 200 204 4 831.18 2.10 561 201 204 3 857.37 1.59 562 201 205 4 849.03 3.08 563 202 205 3 876.14 1.20 564 202 206 4 848.80 3.20 565 203 206 3 858.49 1.65 566 206 229 23 166.38 21.82 567 207 229 22 166.35 21.81 568 208 228 20 166.56 21.82 569 208 229 21 166.37 21.80 570 208 230 22 166.35 21.80 571 209 229 20 166.44 21.76 572 209 230 21 166.22 21.77 573 209 231 22 166.18 21.75 574 209 232 23 166.16 21.75 575 210 214 4 886.25 3.31 576 210 222 12 166.68 21.86 577 210 230 20 166.41 21.75 578 210 231 21 166.21 21.76 579 210 232 22 166.18 21.75 580 211 232 21 166.16 21.75 581 213 218 5 843.16 5.04 582 214 218 4 784.43 34.41 583 214 219 5 317.75 27.30 584 214 231 17 166.47 21.72 585 214 233 19 166.35 21.71 586 214 236 22 165.98 21.68 587 214 245 31 102.50 25.43 588 215 218 3 876.13 11.01 589 215 219 4 725.04 64.09 590 215 236 21 165.97 21.67 591 215 241 26 118.77 32.04 592 215 245 30 103.60 24.88 593 216 241 25 121.77 33.54 594 216 244 28 107.01 26.25 595 216 245 29 103.72 24.82 596 217 241 24 134.77 34.16 597 218 221 3 872.66 0.47 598 218 222 4 842.46 0.10 599 219 222 3 857.43 3.25 600 219 223 4 842.48 0.10 601 219 231 12 167.30 22.07 602 219 245 26 101.77 25.79 603 219 248 29 91.89 24.01 604 220 223 3 858.45 1.48 605 220 224 4 842.48 0.10 606 220 248 28 45.48 0.81 607 220 250 30 45.41 0.84 608 221 224 3 858.75 1.32 609 221 225 4 842.49 0.10 610 222 225 3 862.42 2.09 611 222 228 6 295.93 16.73 612 222 231 9 168.93 21.32 613 223 228 5 266.87 31.27 614 223 229 6 227.85 50.77 615 223 230 7 169.00 21.35 616 223 231 8 168.97 21.34 617 223 248 25 44.98 0.64 618 223 249 26 39.43 0.45 619 225 228 3 860.06 2.12 620 231 245 14 93.14 23.38 621 231 249 18 29.42 1.69 622 233 236 3 902.40 0.88 623 233 237 4 821.63 2.43 624 233 242 9 140.87 6.25 625 233 245 12 101.78 25.70 626 234 237 3 862.14 1.73 627 235 238 3 857.39 0.46 628 235 239 4 853.95 1.26 629 236 239 3 857.20 6.77 630 236 241 5 263.37 28.88 631 236 242 6 259.56 27.13 632 236 245 9 170.62 21.09 633 237 242 5 214.66 4.69 634 239 242 3 874.07 1.43 635 240 243 3 862.37 0.51 636 241 244 3 839.73 0.04 637 241 245 4 844.65 0.14 638 242 245 3 854.90 0.54 639 242 246 4 844.70 0.12 640 243 246 3 859.88 2.91 641 243 247 4 854.70 0.38 642 244 247 3 862.70 4.32 643 244 248 4 844.72 0.13 644 245 248 3 849.19 2.37 645 245 249 4 854.70 0.39 646 246 249 3 862.63 0.30 647 247 250 3 866.65 1.71