BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 169.56 0.24 2 4 204 200 169.28 0.37 3 4 205 201 165.94 0.45 4 4 224 220 112.09 0.57 5 4 227 223 111.95 0.56 6 5 36 31 546.82 1.60 7 5 37 32 546.83 1.60 8 5 38 33 546.83 1.60 9 5 205 200 168.91 0.55 10 6 37 31 546.83 1.60 11 6 38 32 546.84 1.60 12 6 221 215 112.02 0.57 13 6 227 221 112.03 0.58 14 6 228 222 112.01 0.58 15 6 229 223 112.02 0.58 16 6 246 240 35.08 0.02 17 6 247 241 26.70 0.60 18 7 38 31 546.84 1.60 19 7 40 33 546.86 1.60 20 7 90 83 186.63 0.86 21 7 124 117 178.36 1.19 22 7 161 154 176.98 0.95 23 7 205 198 176.87 0.98 24 7 207 200 168.10 0.35 25 7 227 220 112.12 0.58 26 7 228 221 112.02 0.58 27 7 229 222 112.02 0.58 28 8 37 29 546.95 1.60 29 8 38 30 546.93 1.60 30 8 39 31 546.88 1.60 31 8 40 32 546.88 1.60 32 8 229 221 112.02 0.58 33 8 231 223 112.00 0.58 34 8 240 232 51.88 1.17 35 8 243 235 40.98 0.61 36 8 244 236 36.00 0.42 37 9 40 31 546.88 1.61 38 9 229 220 112.17 0.59 39 9 230 221 112.01 0.58 40 9 231 222 112.01 0.58 41 10 40 30 546.94 1.60 42 10 41 31 546.88 1.61 43 10 42 32 546.88 1.61 44 10 231 221 112.02 0.58 45 10 235 225 77.52 0.51 46 10 240 230 51.93 1.19 47 11 42 31 546.88 1.60 48 11 64 53 237.40 26.31 49 11 92 81 186.93 1.02 50 11 95 84 185.45 0.49 51 11 230 219 113.00 0.49 52 11 231 220 112.12 0.58 53 11 232 221 112.00 0.57 54 11 235 224 80.34 0.10 55 12 15 3 864.18 1.25 56 12 21 9 548.09 1.55 57 12 24 12 547.85 1.56 58 12 25 13 547.86 1.56 59 12 41 29 546.96 1.60 60 12 42 30 546.93 1.60 61 12 43 31 546.91 1.61 62 12 44 32 546.89 1.61 63 12 51 39 451.49 1.53 64 12 235 223 103.67 4.00 65 12 236 224 78.55 0.75 66 13 44 31 546.89 1.60 67 13 64 51 237.69 26.41 68 13 95 82 186.52 0.81 69 13 97 84 186.12 0.68 70 13 170 157 186.35 0.83 71 13 232 219 112.89 0.43 72 15 44 29 546.96 1.60 73 15 236 221 111.79 0.58 74 16 20 4 766.40 27.58 75 16 21 5 563.78 0.98 76 16 47 31 546.92 1.60 77 17 20 3 853.76 1.03 78 17 21 4 766.42 27.59 79 17 47 30 546.93 1.59 80 18 22 4 824.37 1.27 81 18 47 29 546.92 1.59 82 18 50 32 527.25 1.37 83 19 22 3 841.47 2.14 84 19 23 4 839.59 2.31 85 20 23 3 852.52 1.51 86 20 24 4 839.59 2.31 87 21 24 3 848.79 2.29 88 21 25 4 831.24 1.85 89 21 44 23 547.00 1.60 90 21 47 26 546.82 1.55 91 21 50 29 500.04 3.03 92 21 236 215 101.34 2.73 93 22 25 3 856.20 6.00 94 22 26 4 830.36 1.41 95 22 50 28 500.05 3.03 96 23 26 3 851.66 6.05 97 23 27 4 841.79 1.11 98 24 27 3 852.81 0.25 99 24 28 4 844.33 0.15 100 24 235 211 108.79 1.72 101 24 236 212 88.21 1.09 102 24 237 213 81.78 1.43 103 25 28 3 848.16 2.08 104 25 29 4 835.98 4.33 105 25 51 26 454.81 1.20 106 25 54 29 299.71 51.94 107 25 55 30 244.22 24.20 108 26 29 3 855.94 1.34 109 26 30 4 841.97 1.34 110 27 30 3 855.83 1.39 111 27 31 4 835.94 4.36 112 28 31 3 848.11 4.42 113 28 33 5 823.34 1.95 114 28 41 13 547.09 1.62 115 28 55 27 244.06 24.24 116 28 59 31 243.19 24.36 117 28 240 212 55.73 0.61 118 28 241 213 52.12 1.18 119 28 244 216 51.80 1.12 120 29 54 25 244.22 24.19 121 29 55 26 244.04 24.23 122 29 56 27 243.95 24.20 123 31 241 210 52.11 1.17 124 32 241 209 52.01 1.22 125 32 245 213 42.84 0.30 126 33 59 26 239.03 26.07 127 33 241 208 51.80 1.17 128 33 244 211 42.45 0.11 129 33 245 212 41.13 0.33 130 34 37 3 853.12 1.80 131 34 244 210 42.42 0.11 132 34 245 211 38.41 1.25 133 34 248 214 33.91 1.10 134 37 244 207 36.46 0.16 135 38 90 52 187.71 1.35 136 38 205 167 176.97 0.95 137 39 59 20 238.03 26.31 138 39 60 21 238.00 26.32 139 39 240 201 51.89 1.17 140 39 244 205 35.95 0.39 141 40 59 19 238.44 26.18 142 40 60 20 238.00 26.32 143 40 62 22 237.90 26.36 144 40 90 50 186.63 0.86 145 40 124 84 185.16 0.54 146 41 55 14 242.34 24.89 147 41 59 18 239.03 26.11 148 41 60 19 238.91 26.07 149 41 61 20 237.91 26.37 150 41 62 21 237.91 26.37 151 41 240 199 52.02 1.22 152 42 62 20 237.85 26.39 153 42 64 22 237.75 26.40 154 42 92 50 186.94 1.01 155 43 48 5 501.11 21.70 156 43 51 8 474.19 35.16 157 43 62 19 238.24 26.38 158 43 64 21 237.83 26.41 159 44 47 3 842.53 5.28 160 44 48 4 756.18 32.15 161 44 64 20 237.87 26.39 162 45 48 3 840.19 6.45 163 45 78 33 199.21 7.67 164 47 50 3 854.02 2.66 165 47 51 4 834.72 2.39 166 48 51 3 841.93 1.22 167 48 52 4 834.75 2.38 168 48 61 13 241.75 25.09 169 48 62 14 239.86 26.03 170 48 63 15 238.66 26.56 171 48 78 30 205.09 4.74 172 48 86 38 195.53 0.30 173 49 52 3 848.28 4.38 174 49 53 4 844.64 7.30 175 49 86 37 195.61 0.35 176 50 53 3 851.81 3.73 177 50 54 4 844.65 7.30 178 51 54 3 857.15 1.05 179 51 55 4 844.64 7.30 180 51 61 10 242.32 24.80 181 52 55 3 864.09 2.23 182 52 56 4 862.58 1.91 183 52 57 5 842.28 6.24 184 52 59 7 550.74 0.40 185 52 60 8 370.81 87.23 186 52 61 9 242.71 24.62 187 52 86 34 194.93 0.81 188 52 87 35 193.85 1.30 189 55 59 4 830.94 2.35 190 55 61 6 370.92 87.22 191 56 59 3 857.62 0.48 192 61 88 27 226.00 16.57 193 61 89 28 226.00 16.56 194 61 90 29 225.81 16.50 195 62 90 28 225.94 16.56 196 62 91 29 225.99 16.57 197 62 92 30 226.11 16.48 198 63 78 15 244.16 14.85 199 63 83 20 226.67 16.44 200 63 90 27 226.16 16.50 201 63 91 28 226.16 16.50 202 63 92 29 226.16 16.50 203 64 92 28 226.21 16.48 204 65 78 13 244.33 14.88 205 65 83 18 244.02 14.83 206 65 91 26 227.08 16.23 207 65 92 27 226.49 16.52 208 65 93 28 226.49 16.52 209 65 98 33 226.58 16.57 210 65 104 39 233.56 19.17 211 65 112 47 238.11 17.51 212 66 78 12 244.33 14.89 213 66 79 13 244.21 14.88 214 66 80 14 244.20 14.88 215 66 83 17 244.09 14.84 216 66 91 25 228.10 15.84 217 66 93 27 227.25 16.27 218 66 112 46 240.02 16.71 219 66 116 50 242.27 15.59 220 67 74 7 245.66 14.31 221 67 77 10 244.47 14.87 222 67 78 11 244.46 14.87 223 67 80 13 244.34 14.90 224 67 112 45 240.06 16.70 225 67 115 48 243.91 14.91 226 68 108 40 240.12 16.71 227 68 111 43 242.65 15.46 228 68 112 44 240.08 16.70 229 68 115 47 243.48 15.12 230 69 74 5 774.26 40.09 231 69 79 10 244.49 14.86 232 69 115 46 244.03 14.96 233 70 79 9 244.49 14.85 234 70 115 45 244.41 14.87 235 74 77 3 860.44 1.00 236 78 82 4 836.00 3.75 237 78 83 5 835.99 3.75 238 79 82 3 852.38 3.77 239 79 83 4 847.21 1.86 240 80 83 3 847.25 1.84 241 80 84 4 847.21 1.86 242 80 115 35 493.18 31.78 243 80 116 36 356.42 21.93 244 80 119 39 566.14 4.43 245 80 121 41 565.78 4.58 246 81 84 3 858.14 4.78 247 81 85 4 841.71 0.88 248 81 119 38 566.16 4.44 249 82 85 3 847.16 1.89 250 82 86 4 847.16 1.89 251 83 86 3 855.22 3.31 252 83 87 4 836.98 1.48 253 83 88 5 809.70 4.40 254 83 91 8 564.40 3.81 255 83 121 38 579.69 2.12 256 84 87 3 846.50 1.57 257 84 88 4 823.71 1.35 258 84 119 35 589.06 2.44 259 84 121 37 579.49 1.99 260 88 121 33 660.38 2.69 261 89 121 32 660.38 2.69 262 89 122 33 660.38 2.69 263 90 122 32 660.38 2.69 264 90 123 33 660.37 2.69 265 90 124 34 660.33 2.68 266 90 159 69 660.38 2.69 267 90 161 71 660.37 2.69 268 91 116 25 660.46 2.68 269 91 121 30 660.45 2.68 270 91 122 31 660.41 2.68 271 91 123 32 660.40 2.68 272 91 124 33 660.37 2.69 273 92 95 3 903.37 0.12 274 92 124 32 660.39 2.68 275 92 126 34 660.34 2.69 276 93 112 19 660.58 2.69 277 93 113 20 660.50 2.69 278 93 116 23 660.60 2.70 279 93 123 30 660.45 2.69 280 93 124 31 660.43 2.69 281 93 125 32 660.37 2.69 282 93 126 33 660.34 2.71 283 94 99 5 668.51 0.83 284 94 109 15 660.51 2.71 285 94 126 32 660.38 2.68 286 95 98 3 845.70 6.54 287 95 99 4 825.24 3.69 288 95 126 31 660.40 2.69 289 95 165 70 660.34 2.73 290 96 99 3 844.66 0.42 291 96 100 4 824.62 0.40 292 96 165 69 660.35 2.73 293 96 167 71 650.14 1.05 294 96 170 74 564.52 2.98 295 97 100 3 865.57 3.40 296 97 101 4 737.40 37.72 297 97 102 5 664.38 1.22 298 97 170 73 616.33 5.75 299 98 102 4 664.36 1.22 300 98 103 5 664.36 1.22 301 98 104 6 661.06 2.69 302 99 103 4 661.16 2.72 303 99 104 5 661.06 2.69 304 99 106 7 661.09 2.69 305 99 109 10 661.04 2.69 306 99 126 27 660.51 2.71 307 99 127 28 660.46 2.70 308 99 146 47 660.54 2.70 309 99 150 51 660.78 2.69 310 100 130 30 660.35 2.72 311 100 167 67 660.19 2.80 312 104 108 4 832.33 5.00 313 104 109 5 818.20 0.11 314 104 112 8 812.69 2.66 315 105 108 3 841.49 0.89 316 105 109 4 840.38 3.17 317 106 109 3 841.27 2.73 318 106 110 4 832.52 4.91 319 106 146 40 660.64 2.65 320 106 149 43 660.75 2.69 321 107 110 3 851.35 0.12 322 107 111 4 838.65 6.23 323 108 111 3 868.37 0.07 324 108 112 4 851.44 0.16 325 109 112 3 860.13 4.19 326 109 113 4 851.44 0.15 327 109 150 41 660.83 2.72 328 110 113 3 859.68 4.27 329 110 114 4 851.48 0.15 330 110 149 39 660.78 2.69 331 110 153 43 661.19 2.60 332 111 114 3 851.48 0.15 333 111 115 4 851.48 0.14 334 112 115 3 851.49 0.14 335 112 116 4 837.68 6.75 336 113 116 3 851.48 0.14 337 113 117 4 846.05 2.56 338 113 123 10 661.18 2.58 339 113 150 37 661.12 2.58 340 113 154 41 662.81 3.23 341 114 117 3 851.70 0.22 342 114 118 4 840.72 5.45 343 114 153 39 661.39 2.54 344 115 118 3 851.70 0.22 345 115 119 4 840.72 5.46 346 116 119 3 851.71 0.21 347 116 120 4 840.70 5.46 348 116 121 5 804.44 1.38 349 116 123 7 665.12 3.05 350 117 120 3 843.80 3.92 351 117 121 4 811.75 2.27 352 117 123 6 674.30 0.75 353 117 154 37 676.18 1.67 354 117 159 42 679.19 1.02 355 117 160 43 674.35 0.75 356 120 159 39 797.03 0.69 357 121 159 38 797.04 0.69 358 122 159 37 797.03 0.69 359 123 159 36 797.12 0.69 360 123 160 37 797.04 0.69 361 123 161 38 797.04 0.69 362 124 161 37 797.03 0.69 363 124 163 39 797.00 0.68 364 124 207 83 379.76 0.53 365 124 230 106 112.63 0.32 366 125 150 25 797.32 0.68 367 125 161 36 797.11 0.69 368 125 162 37 797.07 0.69 369 125 163 38 797.05 0.69 370 126 163 37 797.06 0.69 371 126 165 39 797.03 0.69 372 126 230 104 113.88 0.07 373 127 143 16 797.49 0.68 374 127 146 19 797.46 0.66 375 127 147 20 797.45 0.66 376 127 150 23 797.45 0.66 377 127 163 36 797.10 0.69 378 127 164 37 797.06 0.69 379 127 165 38 797.06 0.70 380 127 188 61 386.86 1.30 381 128 143 15 797.54 0.66 382 128 146 18 797.53 0.68 383 128 164 36 797.08 0.69 384 128 165 37 797.06 0.69 385 128 166 38 797.01 0.70 386 128 167 39 797.00 0.70 387 129 133 4 815.07 0.69 388 129 134 5 813.71 0.54 389 129 139 10 797.65 0.68 390 129 142 13 797.61 0.69 391 129 164 35 797.16 0.69 392 129 166 37 797.06 0.70 393 129 167 38 797.05 0.69 394 129 168 39 797.04 0.69 395 130 133 3 851.17 0.79 396 130 134 4 833.21 0.83 397 130 167 37 797.06 0.70 398 130 168 38 797.05 0.70 399 131 134 3 843.78 2.90 400 131 135 4 833.21 0.83 401 131 168 37 797.07 0.69 402 132 135 3 851.15 0.79 403 132 136 4 833.22 0.84 404 133 136 3 848.14 2.29 405 133 137 4 833.21 0.84 406 133 138 5 800.27 0.55 407 133 139 6 798.00 0.58 408 133 142 9 797.97 0.58 409 134 137 3 855.18 1.23 410 134 139 5 798.00 0.59 411 134 164 30 797.24 0.69 412 134 168 34 797.09 0.69 413 134 180 46 390.12 1.28 414 134 183 49 390.07 1.29 415 134 184 50 389.82 1.33 416 138 141 3 848.14 6.56 417 138 142 4 841.93 3.60 418 139 142 3 863.94 1.34 419 139 143 4 843.13 3.00 420 139 184 45 387.44 1.33 421 140 143 3 853.50 0.51 422 140 144 4 841.85 3.65 423 140 187 47 387.23 1.26 424 141 144 3 850.43 0.74 425 141 145 4 843.11 2.98 426 142 145 3 846.27 2.82 427 142 146 4 841.88 3.59 428 143 146 3 841.88 3.59 429 143 147 4 831.12 2.51 430 143 164 21 797.13 0.68 431 143 184 41 387.14 1.29 432 143 188 45 387.05 1.26 433 144 147 3 841.82 3.61 434 144 148 4 830.22 7.84 435 144 187 43 387.17 1.25 436 144 188 44 387.09 1.28 437 144 191 47 386.73 1.36 438 145 148 3 847.47 0.78 439 145 149 4 838.25 3.85 440 146 149 3 852.07 1.92 441 146 150 4 845.08 0.43 442 147 150 3 850.07 2.92 443 147 151 4 838.25 3.83 444 147 162 15 797.16 0.69 445 147 188 41 386.92 1.32 446 147 191 44 386.56 1.44 447 148 151 3 842.09 3.81 448 148 152 4 815.40 7.58 449 148 191 43 386.69 1.35 450 149 153 4 840.22 2.87 451 150 153 3 848.75 0.45 452 150 154 4 840.22 2.88 453 150 162 12 797.15 0.69 454 151 154 3 840.22 2.88 455 151 157 6 798.22 0.59 456 151 191 40 386.50 1.46 457 154 157 3 890.86 2.75 458 154 160 6 797.67 0.62 459 156 160 4 851.30 1.35 460 157 160 3 841.84 3.38 461 157 162 5 850.99 1.51 462 157 192 35 384.66 1.39 463 157 196 39 380.59 0.61 464 157 200 43 380.10 0.62 465 157 204 47 379.94 0.57 466 160 204 44 379.89 0.52 467 161 205 44 379.85 0.51 468 161 207 46 379.76 0.53 469 162 192 30 385.68 1.13 470 162 204 42 380.02 0.55 471 162 205 43 379.93 0.55 472 162 206 44 379.79 0.53 473 162 207 45 379.78 0.54 474 163 207 44 379.81 0.53 475 163 230 67 113.63 0.01 476 164 184 20 386.88 1.29 477 164 185 21 386.85 1.30 478 164 188 24 386.85 1.30 479 164 207 43 379.89 0.54 480 164 208 44 379.79 0.54 481 164 209 45 379.75 0.53 482 165 170 5 815.37 2.92 483 165 209 44 379.67 0.50 484 165 230 65 114.06 0.02 485 166 169 3 851.38 1.71 486 166 170 4 835.00 1.01 487 166 208 42 379.95 0.55 488 166 209 43 379.88 0.54 489 167 170 3 856.73 3.43 490 168 172 4 834.52 0.77 491 168 174 6 423.47 4.85 492 168 176 8 390.37 1.29 493 168 180 12 390.22 1.23 494 169 172 3 848.31 0.76 495 169 174 5 390.37 1.29 496 169 175 6 390.24 1.36 497 169 208 39 380.06 0.54 498 169 210 41 379.74 0.55 499 169 211 42 379.74 0.55 500 170 210 40 379.80 0.52 501 172 211 39 379.88 0.54 502 173 211 38 379.88 0.54 503 174 211 37 379.88 0.53 504 175 208 33 380.15 0.54 505 175 210 35 379.93 0.56 506 175 211 36 379.93 0.56 507 175 216 41 346.44 0.61 508 175 220 45 262.42 18.08 509 176 180 4 841.75 7.16 510 176 181 5 729.17 34.08 511 176 208 32 380.15 0.56 512 176 220 44 262.23 18.02 513 177 180 3 847.18 4.44 514 177 181 4 834.61 1.53 515 178 181 3 846.88 4.59 516 178 182 4 834.61 1.53 517 178 219 41 262.01 17.96 518 178 220 42 254.93 14.46 519 178 223 45 162.09 23.71 520 179 182 3 846.88 4.59 521 179 183 4 849.99 3.04 522 180 183 3 857.95 6.50 523 180 184 4 842.01 0.96 524 181 184 3 864.51 3.23 525 181 185 4 840.80 1.56 526 181 208 27 380.23 0.53 527 181 220 39 199.28 42.27 528 181 226 45 114.88 0.19 529 182 185 3 863.97 3.50 530 182 186 4 840.75 1.57 531 182 223 41 115.25 0.33 532 182 224 42 115.25 0.33 533 182 225 43 114.92 0.22 534 183 186 3 844.00 3.00 535 183 187 4 837.88 0.12 536 184 187 3 863.37 0.68 537 184 188 4 837.88 0.12 538 185 188 3 848.64 0.72 539 185 189 4 843.13 2.03 540 185 206 21 380.62 0.52 541 185 207 22 380.60 0.51 542 185 208 23 380.55 0.49 543 185 225 40 114.66 0.24 544 185 226 41 114.61 0.23 545 186 189 3 848.77 0.79 546 186 190 4 843.16 2.02 547 186 225 39 114.69 0.26 548 187 190 3 848.77 0.79 549 187 191 4 843.20 2.00 550 188 191 3 837.60 2.62 551 188 192 4 835.12 2.02 552 188 206 18 380.62 0.52 553 189 192 3 843.76 3.04 554 189 193 4 836.32 1.42 555 189 201 12 380.91 0.55 556 189 204 15 380.68 0.53 557 189 206 17 380.62 0.52 558 189 225 36 114.64 0.24 559 189 227 38 114.62 0.22 560 190 193 3 841.65 4.08 561 190 194 4 836.03 1.27 562 191 194 3 845.32 2.27 563 191 195 4 831.24 1.12 564 191 196 5 827.84 2.83 565 192 195 3 839.99 3.25 566 192 196 4 827.80 2.85 567 192 197 5 827.78 2.85 568 192 200 8 396.42 2.04 569 192 201 9 381.05 0.58 570 192 204 12 380.71 0.51 571 192 206 14 380.64 0.52 572 193 197 4 679.43 59.94 573 193 198 5 402.53 0.99 574 193 201 8 384.75 2.42 575 196 200 4 841.05 4.62 576 197 200 3 852.77 3.87 577 197 201 4 844.79 2.75 578 198 201 3 847.53 2.83 579 198 202 4 844.79 2.75 580 199 202 3 844.78 2.75 581 199 203 4 837.32 0.98 582 200 203 3 857.58 0.13 583 200 204 4 815.25 8.27 584 205 227 22 114.42 0.19 585 206 226 20 114.59 0.21 586 206 227 21 114.48 0.18 587 207 227 20 114.52 0.17 588 207 228 21 114.34 0.14 589 207 229 22 114.03 0.01 590 207 230 23 114.01 0.01 591 208 212 4 866.89 2.43 592 208 220 12 115.38 0.24 593 208 226 18 114.78 0.12 594 208 228 20 114.53 0.16 595 208 229 21 114.09 0.01 596 208 230 22 114.08 0.01 597 209 212 3 881.22 3.10 598 209 230 21 114.06 0.02 599 211 216 5 834.11 0.03 600 212 216 4 834.11 0.03 601 212 217 5 640.76 86.00 602 212 229 17 114.81 0.10 603 212 231 19 114.52 0.15 604 212 234 22 113.87 0.06 605 212 243 31 47.57 0.19 606 213 216 3 848.37 0.99 607 213 217 4 817.99 8.09 608 213 234 21 114.20 0.21 609 213 239 26 53.91 0.28 610 213 243 30 47.63 0.17 611 214 217 3 856.59 2.50 612 214 239 25 52.84 0.80 613 214 242 28 51.71 1.08 614 214 243 29 48.48 0.26 615 216 219 3 882.15 3.56 616 216 220 4 833.32 2.47 617 217 220 3 853.58 2.72 618 217 221 4 843.14 2.43 619 217 229 12 114.90 0.12 620 217 243 26 43.71 0.52 621 217 246 29 36.20 0.51 622 218 221 3 850.52 3.42 623 218 222 4 843.15 2.43 624 218 245 27 40.56 0.88 625 218 246 28 35.78 0.30 626 218 247 29 35.78 0.30 627 218 248 30 35.78 0.30 628 219 222 3 856.08 0.64 629 219 223 4 849.22 2.06 630 220 223 3 860.17 1.40 631 220 226 6 261.71 68.56 632 220 229 9 114.94 0.20 633 221 226 5 115.06 0.18 634 221 229 8 114.87 0.27 635 221 246 25 35.77 0.30 636 221 247 26 35.63 0.35 637 223 226 3 844.76 2.93 638 226 229 3 1008.56 5.33 639 229 243 14 41.31 0.44 640 229 246 17 35.77 0.30 641 231 234 3 874.55 2.35 642 231 235 4 827.01 6.70 643 231 240 9 51.88 1.16 644 231 243 12 42.45 0.11 645 232 235 3 864.21 4.62 646 233 236 3 864.21 4.62 647 233 237 4 857.49 1.26 648 234 237 3 859.68 2.31 649 234 239 5 418.46 0.56 650 234 240 6 87.22 16.39 651 234 243 9 84.94 17.73 652 235 240 5 383.69 1.29 653 237 240 3 858.75 2.79 654 238 241 3 858.75 2.79 655 239 242 3 845.83 3.50 656 239 243 4 834.34 1.63 657 240 243 3 841.41 1.91 658 240 244 4 831.06 3.27 659 241 244 3 838.04 5.15 660 241 245 4 826.12 0.81 661 242 245 3 836.05 2.04 662 242 246 4 748.11 39.82 663 243 246 3 821.58 4.33 664 245 248 3 838.78 0.90