BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 225 222 114.95 0.37 2 3 226 223 114.95 0.38 3 3 228 225 113.83 0.89 4 3 229 226 113.66 0.96 5 4 204 200 143.04 13.61 6 4 205 201 143.03 13.61 7 4 206 202 143.02 13.61 8 4 207 203 142.40 13.42 9 4 229 225 115.61 0.22 10 5 36 31 563.66 53.87 11 5 37 32 563.62 53.89 12 5 38 33 563.63 53.88 13 5 207 202 143.11 13.65 14 6 36 30 563.70 53.88 15 6 37 31 563.65 53.88 16 6 38 32 563.64 53.88 17 6 223 217 116.08 0.14 18 6 229 223 116.08 0.14 19 6 249 243 22.46 1.55 20 7 37 30 563.67 53.88 21 7 38 31 563.64 53.88 22 7 40 33 563.66 53.87 23 7 90 83 159.16 18.29 24 7 163 156 147.25 14.14 25 7 207 200 145.35 13.44 26 7 209 202 143.02 13.61 27 7 229 222 116.31 0.06 28 7 230 223 116.08 0.14 29 7 231 224 115.95 0.14 30 8 37 29 563.72 53.87 31 8 38 30 563.70 53.87 32 8 39 31 563.66 53.87 33 8 40 32 563.66 53.87 34 8 231 223 116.09 0.14 35 8 242 234 52.05 1.65 36 8 246 238 38.77 0.23 37 8 249 241 25.78 0.10 38 9 40 31 563.66 53.87 39 9 124 115 155.03 17.01 40 9 231 222 116.39 0.03 41 9 232 223 115.97 0.14 42 9 233 224 115.94 0.14 43 9 242 233 52.18 1.69 44 10 40 30 563.69 53.88 45 10 41 31 563.68 53.87 46 10 42 32 563.68 53.87 47 10 233 223 116.02 0.18 48 10 237 227 84.16 0.16 49 10 242 232 52.18 1.69 50 11 42 31 563.68 53.87 51 11 65 54 456.59 0.72 52 11 95 84 155.84 17.14 53 11 232 221 118.41 0.14 54 11 233 222 116.45 0.01 55 11 234 223 115.95 0.14 56 11 237 226 92.42 1.02 57 12 15 3 888.97 1.96 58 12 21 9 564.63 53.98 59 12 24 12 564.60 53.97 60 12 25 13 564.60 53.97 61 12 28 16 564.60 53.97 62 12 41 29 563.82 53.83 63 12 42 30 563.78 53.82 64 12 43 31 563.71 53.85 65 12 65 53 457.56 1.08 66 12 237 225 113.09 0.94 67 12 238 226 88.17 1.23 68 13 44 31 563.78 53.82 69 13 65 52 457.54 1.08 70 13 95 82 158.98 18.32 71 13 97 84 156.09 17.21 72 13 234 221 118.53 0.19 73 14 44 30 563.78 53.82 74 15 44 29 563.82 53.80 75 15 238 223 115.86 0.15 76 16 20 4 843.06 1.85 77 16 21 5 840.77 1.53 78 16 47 31 563.86 53.71 79 17 20 3 851.14 1.41 80 17 21 4 842.89 0.53 81 18 21 3 861.66 4.08 82 18 22 4 848.63 2.42 83 18 50 32 546.44 44.92 84 19 22 3 858.88 2.69 85 19 23 4 849.02 2.22 86 20 23 3 859.13 2.58 87 20 24 4 857.42 1.99 88 21 24 3 857.43 1.99 89 21 25 4 857.74 2.15 90 21 47 26 563.36 53.38 91 21 238 217 112.48 1.33 92 22 25 3 857.75 2.15 93 22 26 4 854.18 3.94 94 22 50 28 528.84 34.55 95 22 54 32 461.98 1.11 96 23 26 3 854.66 3.70 97 23 27 4 854.19 3.95 98 24 27 3 864.92 1.42 99 24 28 4 854.09 4.00 100 24 237 213 113.14 1.23 101 24 238 214 107.78 0.76 102 24 239 215 86.98 1.16 103 25 28 3 854.09 4.00 104 25 29 4 853.52 4.28 105 25 51 26 504.38 22.30 106 25 54 29 461.30 0.71 107 25 55 30 461.27 0.71 108 26 29 3 872.74 9.96 109 26 30 4 853.53 4.28 110 26 54 28 461.51 0.60 111 27 30 3 882.76 4.96 112 27 31 4 857.35 2.37 113 27 240 213 79.45 0.29 114 27 243 216 68.94 0.24 115 28 31 3 873.01 5.46 116 28 41 13 565.75 52.88 117 28 55 27 461.56 0.57 118 28 57 29 460.84 0.20 119 28 60 32 460.74 0.18 120 28 242 214 68.94 0.24 121 28 243 215 54.07 0.73 122 29 54 25 461.59 0.57 123 29 55 26 461.56 0.56 124 29 56 27 461.49 0.52 125 29 57 28 460.89 0.23 126 31 57 26 460.91 0.16 127 31 243 212 51.37 1.27 128 31 247 216 52.41 0.49 129 32 247 215 49.55 0.51 130 33 37 4 872.40 0.53 131 33 39 6 744.73 36.43 132 33 57 24 501.07 20.01 133 33 59 26 488.53 13.83 134 33 60 27 488.54 13.83 135 33 246 213 49.55 0.51 136 34 59 25 511.42 25.27 137 35 59 24 512.12 25.64 138 35 246 211 48.69 0.20 139 35 247 212 45.47 0.22 140 35 249 214 36.45 1.29 141 35 250 215 23.81 0.88 142 37 59 22 511.83 25.82 143 37 246 209 39.66 0.21 144 37 249 212 28.09 0.29 145 38 59 21 511.86 25.82 146 38 61 23 511.78 25.83 147 38 90 52 159.19 18.39 148 39 59 20 511.86 25.82 149 39 60 21 511.80 25.83 150 39 61 22 511.79 25.84 151 39 246 207 38.77 0.23 152 40 61 21 511.83 25.82 153 40 63 23 511.78 25.83 154 40 90 50 160.24 18.83 155 41 51 10 513.15 25.27 156 41 55 14 512.77 25.46 157 41 60 19 512.02 25.80 158 41 61 20 511.98 25.79 159 41 62 21 511.81 25.82 160 41 63 22 511.79 25.83 161 42 63 21 511.79 25.83 162 42 65 23 511.04 26.19 163 42 92 50 160.28 18.82 164 43 47 4 834.51 3.37 165 43 48 5 618.93 27.30 166 43 63 20 512.00 25.80 167 43 65 22 511.81 25.82 168 44 47 3 863.25 3.18 169 44 48 4 845.05 8.63 170 44 65 21 511.87 25.84 171 45 48 3 859.62 1.35 172 45 64 19 512.01 25.80 173 45 66 21 511.98 25.80 174 45 78 33 216.48 0.17 175 45 83 38 215.19 0.73 176 47 50 3 866.85 2.33 177 47 51 4 844.85 7.55 178 48 51 3 866.39 2.56 179 48 52 4 855.44 2.25 180 48 62 14 512.31 25.73 181 48 63 15 512.15 25.80 182 48 64 16 512.09 25.78 183 48 83 35 210.62 0.28 184 48 86 38 208.71 1.24 185 48 88 40 204.32 1.31 186 49 52 3 866.83 2.35 187 49 53 4 850.51 4.76 188 49 86 37 208.69 1.25 189 50 53 3 856.53 2.80 190 50 54 4 850.44 4.73 191 51 54 3 869.84 1.64 192 51 55 4 826.81 3.56 193 51 62 11 512.37 25.74 194 52 62 10 512.37 25.73 195 52 86 34 208.63 1.23 196 55 60 5 557.30 3.29 197 55 62 7 512.50 25.68 198 57 60 3 866.53 0.03 199 60 246 186 48.41 0.36 200 61 90 29 159.28 18.36 201 62 88 26 160.74 18.82 202 62 90 28 160.43 18.89 203 63 88 25 160.94 18.80 204 63 90 27 160.43 18.89 205 63 91 28 160.56 18.91 206 64 83 19 208.53 1.26 207 64 88 24 187.09 6.21 208 64 90 26 160.69 18.89 209 64 91 27 160.67 18.89 210 64 92 28 160.69 18.89 211 65 92 27 160.88 18.82 212 66 83 17 216.40 0.17 213 66 91 25 161.45 18.98 214 66 92 26 161.41 19.00 215 66 93 27 161.36 18.99 216 66 98 32 161.53 19.08 217 66 112 46 204.86 2.10 218 67 78 11 216.58 0.21 219 67 79 12 216.58 0.21 220 67 80 13 216.52 0.23 221 67 83 16 216.43 0.18 222 67 91 24 191.08 5.30 223 67 93 26 169.99 14.97 224 67 112 45 205.51 1.78 225 68 74 6 218.75 1.10 226 68 77 9 216.80 0.29 227 68 78 10 216.79 0.29 228 68 79 11 216.71 0.26 229 68 80 12 216.70 0.26 230 68 112 44 205.52 1.78 231 69 80 11 216.72 0.25 232 69 81 12 216.75 0.27 233 69 115 46 212.47 0.53 234 70 79 9 216.84 0.30 235 70 81 11 216.84 0.30 236 71 74 3 971.74 1.07 237 71 79 8 216.87 0.29 238 71 82 11 216.85 0.28 239 74 77 3 873.02 2.91 240 78 82 4 847.64 4.12 241 78 83 5 847.64 4.12 242 79 82 3 871.40 3.69 243 79 83 4 847.66 4.12 244 80 83 3 855.56 1.76 245 80 84 4 847.68 4.13 246 80 91 11 390.22 36.07 247 80 115 35 391.14 35.62 248 80 116 36 390.23 36.07 249 80 121 41 464.32 53.46 250 81 84 3 867.72 8.20 251 81 85 4 847.68 4.13 252 82 85 3 859.70 1.87 253 82 86 4 847.68 4.13 254 83 86 3 853.63 1.17 255 83 87 4 843.41 6.21 256 83 88 5 664.31 6.25 257 83 91 8 478.59 44.76 258 83 121 38 539.67 26.39 259 84 87 3 850.58 2.62 260 84 88 4 826.05 1.79 261 84 119 35 539.95 26.25 262 84 121 37 539.66 26.39 263 88 121 33 597.45 26.57 264 89 121 32 597.44 26.58 265 89 122 33 597.42 26.57 266 90 121 31 597.55 26.59 267 90 122 32 597.43 26.57 268 90 124 34 597.44 26.58 269 91 116 25 597.71 26.57 270 91 121 30 597.66 26.56 271 91 122 31 597.61 26.56 272 91 123 32 597.45 26.58 273 91 124 33 597.45 26.58 274 92 95 3 919.08 4.05 275 92 124 32 597.45 26.59 276 92 126 34 597.43 26.58 277 92 232 140 123.44 2.21 278 93 112 19 597.84 26.55 279 93 113 20 597.71 26.57 280 93 116 23 597.89 26.56 281 93 123 30 597.66 26.56 282 93 124 31 597.59 26.57 283 93 125 32 597.54 26.57 284 93 126 33 597.44 26.58 285 94 99 5 765.52 32.18 286 94 104 10 597.74 26.57 287 94 109 15 597.72 26.56 288 94 126 32 597.50 26.55 289 95 98 3 871.86 6.15 290 95 99 4 844.20 1.71 291 95 126 31 597.57 26.57 292 95 167 72 597.49 26.55 293 95 232 137 123.82 2.08 294 96 99 3 845.54 1.08 295 96 100 4 839.81 3.91 296 96 167 71 597.48 26.54 297 96 169 73 596.78 26.50 298 97 100 3 855.62 6.12 299 97 101 4 839.78 3.90 300 97 102 5 838.76 4.41 301 98 102 4 683.71 68.39 302 98 103 5 683.71 68.39 303 98 104 6 598.23 26.48 304 99 104 5 598.53 26.73 305 99 106 7 598.54 26.73 306 99 109 10 598.12 26.52 307 99 126 27 597.81 26.59 308 99 127 28 597.78 26.59 309 99 146 47 597.88 26.57 310 103 108 5 830.00 4.42 311 104 108 4 830.00 4.42 312 104 109 5 829.97 4.42 313 104 112 8 812.43 2.83 314 105 108 3 862.04 6.30 315 105 109 4 834.04 6.42 316 106 109 3 849.19 2.94 317 106 110 4 841.94 0.53 318 106 145 39 598.08 26.59 319 106 146 40 598.03 26.57 320 106 149 43 598.22 26.55 321 107 110 3 851.98 4.48 322 107 111 4 841.94 0.53 323 108 111 3 870.38 0.77 324 108 112 4 841.98 2.56 325 109 112 3 853.89 3.40 326 109 113 4 841.98 2.56 327 110 113 3 850.17 1.54 328 110 114 4 845.92 4.53 329 110 149 39 598.39 26.54 330 110 150 40 598.26 26.56 331 110 152 42 599.68 27.07 332 110 153 43 599.69 27.07 333 111 114 3 869.52 1.26 334 111 115 4 845.23 5.67 335 111 153 42 599.91 27.12 336 112 115 3 858.78 1.10 337 112 116 4 848.00 4.29 338 113 116 3 855.09 0.91 339 113 117 4 849.31 2.87 340 113 123 10 598.88 26.55 341 113 125 12 598.32 26.56 342 113 150 37 598.84 26.66 343 113 154 41 606.75 23.75 344 114 117 3 850.24 3.33 345 114 118 4 849.38 2.91 346 114 153 39 606.42 23.90 347 114 154 40 606.44 23.89 348 115 118 3 849.69 2.76 349 115 119 4 851.46 1.79 350 116 119 3 851.93 1.63 351 116 120 4 844.18 5.43 352 116 121 5 796.68 4.63 353 116 123 7 610.88 21.67 354 117 120 3 851.16 3.11 355 117 121 4 824.02 4.02 356 117 123 6 630.54 28.27 357 117 154 37 690.97 1.93 358 117 158 41 690.97 1.93 359 117 161 44 691.69 2.33 360 117 162 45 691.68 2.33 361 121 161 40 783.86 1.41 362 122 160 38 784.00 1.42 363 122 161 39 783.86 1.41 364 122 163 41 783.88 1.41 365 122 207 85 299.72 0.97 366 123 161 38 783.99 1.42 367 123 162 39 783.91 1.41 368 123 163 40 783.90 1.40 369 124 163 39 783.91 1.40 370 124 165 41 783.83 1.40 371 124 209 85 297.68 1.94 372 124 232 108 123.59 2.19 373 125 150 25 784.20 1.41 374 125 163 38 783.96 1.40 375 125 164 39 783.91 1.40 376 125 165 40 783.90 1.40 377 126 165 39 783.91 1.40 378 126 167 41 783.89 1.40 379 126 232 106 123.80 2.08 380 127 143 16 784.61 1.39 381 127 146 19 784.58 1.39 382 127 147 20 784.57 1.39 383 127 150 23 784.55 1.39 384 127 164 37 784.05 1.42 385 127 165 38 783.97 1.41 386 127 166 39 783.92 1.39 387 127 167 40 783.91 1.39 388 127 190 63 318.01 5.24 389 128 143 15 784.63 1.40 390 128 146 18 784.62 1.40 391 128 166 38 783.93 1.39 392 128 167 39 783.93 1.40 393 128 169 41 783.83 1.39 394 129 133 4 840.29 4.54 395 129 134 5 827.05 2.09 396 129 139 10 784.73 1.40 397 129 142 13 784.70 1.38 398 129 143 14 784.64 1.40 399 129 166 37 784.03 1.41 400 129 169 40 783.86 1.40 401 129 170 41 783.83 1.39 402 130 133 3 840.99 4.70 403 130 134 4 840.37 4.54 404 130 169 39 783.84 1.40 405 130 170 40 783.81 1.40 406 131 134 3 861.71 3.21 407 131 135 4 840.60 4.66 408 131 170 39 783.83 1.40 409 131 174 43 739.11 0.17 410 131 176 45 685.98 3.83 411 132 135 3 862.39 2.87 412 132 136 4 851.34 9.99 413 132 138 6 825.98 2.63 414 133 136 3 867.49 2.45 415 133 137 4 840.92 4.26 416 133 138 5 799.07 6.22 417 133 139 6 795.28 6.48 418 133 142 9 784.99 1.34 419 134 137 3 860.51 5.94 420 134 139 5 795.26 6.48 421 134 166 32 784.12 1.41 422 134 170 36 783.89 1.41 423 134 182 48 319.86 5.07 424 138 141 3 857.12 2.88 425 138 142 4 846.54 1.60 426 139 142 3 857.12 2.89 427 139 143 4 841.87 3.94 428 140 143 3 857.14 2.88 429 140 144 4 836.75 1.40 430 140 189 49 318.44 5.40 431 141 144 3 855.42 2.52 432 141 145 4 829.30 2.33 433 142 145 3 852.79 2.13 434 142 146 4 849.52 3.75 435 143 146 3 854.09 4.01 436 143 147 4 848.01 7.04 437 143 166 23 784.02 1.42 438 143 189 46 318.44 5.40 439 143 190 47 318.39 5.41 440 144 147 3 854.09 4.01 441 144 148 4 846.44 7.83 442 144 189 45 318.44 5.40 443 144 190 46 318.42 5.41 444 144 193 49 318.34 5.42 445 145 148 3 857.87 2.12 446 145 149 4 850.18 5.95 447 146 149 3 884.00 3.08 448 146 150 4 856.44 2.83 449 147 150 3 867.84 5.00 450 147 151 4 845.11 2.84 451 147 159 12 784.32 1.42 452 147 164 17 784.08 1.41 453 147 190 43 318.36 5.41 454 147 193 46 318.20 5.36 455 147 194 47 317.90 5.24 456 148 151 3 850.40 0.21 457 148 152 4 842.83 3.98 458 148 193 45 318.20 5.36 459 149 152 3 857.21 2.34 460 149 153 4 857.13 2.37 461 150 153 3 869.65 2.44 462 150 154 4 857.13 2.37 463 151 154 3 859.61 0.55 464 151 159 8 788.53 0.45 465 151 193 42 318.19 5.35 466 151 198 47 302.88 0.51 467 154 158 4 846.74 2.10 468 154 159 5 835.16 4.79 469 154 162 8 784.75 1.31 470 155 158 3 857.20 2.38 471 155 159 4 857.22 2.37 472 156 159 3 877.87 2.62 473 156 198 42 302.85 0.49 474 156 199 43 302.83 0.49 475 156 201 45 302.82 0.49 476 156 202 46 302.61 0.47 477 157 160 3 887.46 2.18 478 158 161 3 889.10 0.45 479 158 162 4 861.71 5.28 480 159 162 3 877.64 5.98 481 159 164 5 876.05 6.78 482 159 198 39 302.89 0.52 483 159 202 43 302.47 0.42 484 159 206 47 300.67 1.19 485 162 206 44 300.39 1.23 486 163 206 43 300.50 1.28 487 163 207 44 299.73 0.96 488 163 209 46 297.68 1.94 489 164 190 26 317.87 5.23 490 164 206 42 301.54 0.78 491 164 207 43 300.38 1.22 492 164 208 44 297.89 1.84 493 164 209 45 297.68 1.94 494 165 209 44 297.67 1.94 495 165 232 67 123.60 2.19 496 166 186 20 318.38 5.41 497 166 187 21 318.27 5.38 498 166 190 24 317.94 5.21 499 166 209 43 297.68 1.94 500 166 210 44 297.65 1.94 501 166 211 45 297.15 2.08 502 167 211 44 296.95 2.02 503 168 171 3 871.25 2.55 504 168 172 4 840.08 0.76 505 168 210 42 299.56 1.01 506 168 211 43 297.60 1.97 507 168 212 44 296.93 2.00 508 169 172 3 863.83 0.99 509 169 174 5 843.84 2.64 510 170 174 4 832.38 8.37 511 170 176 6 610.67 26.59 512 170 178 8 320.46 4.80 513 170 182 12 319.82 5.08 514 171 174 3 858.75 4.81 515 171 176 5 410.02 32.77 516 171 178 7 320.43 4.78 517 172 212 40 296.97 2.02 518 172 213 41 296.97 2.02 519 178 182 4 848.37 0.42 520 178 210 32 301.59 0.82 521 179 182 3 855.72 3.25 522 179 183 4 843.61 2.80 523 180 183 3 843.73 2.74 524 180 184 4 855.72 3.26 525 180 221 41 143.97 10.67 526 180 222 42 143.95 10.66 527 180 225 45 141.34 9.72 528 181 184 3 864.21 6.36 529 181 185 4 855.72 3.26 530 182 185 3 859.33 3.47 531 182 186 4 854.91 3.67 532 183 186 3 860.01 3.12 533 183 187 4 855.75 3.25 534 183 210 27 301.69 0.86 535 183 222 39 143.95 10.66 536 184 187 3 865.12 5.68 537 184 188 4 844.19 2.54 538 184 225 41 129.84 4.52 539 184 226 42 129.54 4.37 540 184 227 43 129.22 4.25 541 185 188 3 870.40 8.65 542 185 189 4 853.96 2.35 543 186 189 3 860.51 0.98 544 186 190 4 856.96 0.84 545 187 190 3 874.28 6.71 546 187 191 4 852.96 1.15 547 187 208 21 301.95 0.97 548 187 209 22 301.94 0.97 549 187 210 23 301.85 0.94 550 187 227 40 127.93 3.66 551 187 228 41 127.94 3.67 552 188 191 3 878.96 4.37 553 188 192 4 852.96 1.15 554 188 227 39 127.95 3.67 555 189 192 3 878.96 4.37 556 189 193 4 842.88 3.90 557 190 193 3 852.97 1.13 558 190 194 4 842.33 4.17 559 190 208 18 301.70 0.85 560 191 194 3 852.97 1.14 561 191 195 4 829.23 1.88 562 191 203 12 303.12 0.48 563 191 206 15 302.76 0.59 564 191 208 17 302.54 0.63 565 191 227 36 127.94 3.67 566 191 229 38 127.89 3.69 567 192 195 3 855.43 2.68 568 192 196 4 844.52 2.78 569 193 196 3 855.98 2.94 570 193 197 4 839.82 0.43 571 193 198 5 827.25 1.37 572 194 197 3 864.91 1.63 573 194 198 4 809.81 2.77 574 194 199 5 809.47 2.94 575 194 202 8 566.94 6.06 576 194 203 9 306.74 1.28 577 194 206 12 302.75 0.58 578 195 199 4 685.06 65.14 579 195 200 5 454.85 49.97 580 195 203 8 323.37 3.37 581 198 202 4 842.52 4.61 582 199 202 3 853.90 1.08 583 199 203 4 858.31 3.29 584 200 203 3 866.63 0.88 585 200 204 4 843.46 10.71 586 201 204 3 872.45 2.02 587 201 205 4 858.31 3.29 588 202 205 3 871.48 3.29 589 202 206 4 829.51 1.99 590 203 206 3 843.47 4.98 591 206 229 23 127.60 3.62 592 207 229 22 127.56 3.60 593 208 228 20 127.64 3.60 594 208 229 21 127.53 3.58 595 209 229 20 127.53 3.58 596 209 230 21 127.16 3.44 597 209 231 22 124.28 2.08 598 209 232 23 123.99 2.12 599 210 214 4 870.94 3.72 600 210 222 12 140.34 9.92 601 210 228 18 127.66 3.60 602 210 230 20 127.41 3.52 603 210 231 21 127.06 3.46 604 210 232 22 127.08 3.47 605 211 232 21 124.16 2.01 606 213 218 5 812.12 9.67 607 214 218 4 812.12 9.67 608 214 219 5 334.25 3.21 609 214 222 8 334.22 3.21 610 214 231 17 127.24 3.40 611 214 233 19 124.38 2.02 612 214 236 22 123.85 2.10 613 214 245 31 50.27 0.85 614 215 218 3 857.40 2.00 615 215 219 4 685.46 66.59 616 215 236 21 123.91 2.07 617 215 241 26 58.62 1.51 618 215 245 30 50.28 0.86 619 216 241 25 52.18 1.70 620 216 244 28 51.01 1.20 621 216 245 29 50.47 0.96 622 218 221 3 864.93 0.88 623 218 222 4 852.35 1.19 624 219 222 3 858.95 2.11 625 219 223 4 851.99 1.36 626 219 231 12 129.38 4.33 627 219 245 26 48.22 0.29 628 219 248 29 36.58 1.33 629 220 223 3 864.70 1.00 630 220 224 4 844.36 5.19 631 220 248 28 36.56 1.32 632 220 250 30 36.55 1.32 633 221 224 3 862.72 1.98 634 221 225 4 850.24 4.26 635 222 225 3 872.66 3.05 636 222 228 6 394.40 35.91 637 222 231 9 134.18 6.71 638 223 228 5 246.75 47.40 639 223 229 6 134.40 6.70 640 223 230 7 134.32 6.67 641 223 231 8 134.31 6.68 642 223 248 25 36.55 1.31 643 223 249 26 23.66 0.11 644 225 228 3 850.16 1.04 645 228 231 3 1046.69 0.53 646 231 245 14 46.64 0.26 647 231 249 18 26.09 0.25 648 233 236 3 887.08 1.98 649 233 237 4 851.25 5.70 650 233 242 9 131.36 4.24 651 233 245 12 48.67 0.06 652 234 237 3 887.64 2.33 653 235 238 3 894.28 0.99 654 235 239 4 868.39 0.99 655 236 239 3 875.37 4.48 656 236 241 5 569.06 0.97 657 236 242 6 225.07 50.08 658 236 245 9 224.22 50.49 659 237 242 5 224.28 50.48 660 239 242 3 875.74 5.57 661 240 243 3 878.32 4.28 662 241 244 3 861.46 2.98 663 241 245 4 829.48 3.92 664 242 245 3 852.44 1.53 665 242 246 4 829.56 3.97 666 243 246 3 854.32 2.48 667 243 247 4 838.78 0.65 668 244 247 3 849.21 4.57 669 244 248 4 838.78 0.65 670 245 248 3 848.67 0.49 671 245 249 4 848.66 0.48 672 246 249 3 871.25 3.00 673 247 250 3 882.69 6.48