BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 112.39 1.40 2 3 228 225 112.26 1.34 3 3 229 226 110.91 1.92 4 4 204 200 147.78 17.42 5 4 205 201 141.47 14.93 6 4 206 202 141.18 15.04 7 4 207 203 139.39 14.29 8 4 229 225 113.28 1.33 9 5 36 31 381.36 38.46 10 5 37 32 381.33 38.47 11 5 38 33 381.32 38.47 12 5 207 202 141.19 15.05 13 6 36 30 381.51 38.48 14 6 37 31 381.35 38.46 15 6 38 32 381.35 38.46 16 6 223 217 113.72 1.44 17 6 229 223 113.72 1.44 18 6 249 243 21.78 0.52 19 7 37 30 381.43 38.45 20 7 38 31 381.35 38.46 21 7 40 33 381.38 38.48 22 7 163 156 148.71 17.23 23 7 207 200 148.38 17.32 24 7 209 202 140.17 14.54 25 7 229 222 114.85 1.01 26 7 230 223 113.75 1.43 27 7 231 224 113.72 1.44 28 8 37 29 381.58 38.51 29 8 38 30 381.50 38.48 30 8 39 31 381.36 38.47 31 8 40 32 381.43 38.47 32 8 231 223 113.79 1.41 33 8 242 234 52.05 1.09 34 8 246 238 39.17 0.24 35 9 40 31 381.42 38.50 36 9 124 115 151.94 17.30 37 9 209 200 148.63 17.21 38 9 231 222 114.30 1.29 39 9 232 223 113.70 1.44 40 9 233 224 113.70 1.44 41 9 242 233 52.25 1.16 42 10 40 30 381.56 38.52 43 10 41 31 381.54 38.51 44 10 42 32 381.56 38.52 45 10 233 223 113.74 1.43 46 10 237 227 83.01 0.29 47 10 242 232 52.31 1.19 48 11 42 31 381.58 38.52 49 11 65 54 380.88 38.37 50 11 95 84 152.25 17.45 51 11 232 221 115.89 0.92 52 11 233 222 113.98 1.32 53 11 234 223 113.73 1.42 54 11 237 226 86.81 0.29 55 12 15 3 871.87 1.68 56 12 21 9 383.46 38.42 57 12 24 12 383.37 38.41 58 12 25 13 383.36 38.41 59 12 28 16 382.61 38.55 60 12 41 29 381.69 38.54 61 12 42 30 381.67 38.55 62 12 43 31 381.64 38.54 63 12 65 53 381.12 38.36 64 12 237 225 110.11 2.09 65 12 238 226 84.88 0.41 66 13 44 31 381.71 38.52 67 13 65 52 381.31 38.47 68 13 95 82 153.51 17.22 69 13 97 84 152.58 17.41 70 13 234 221 116.55 0.62 71 14 44 30 381.78 38.52 72 15 44 29 381.81 38.51 73 15 238 223 113.62 1.41 74 16 21 5 829.63 3.53 75 16 47 31 381.80 38.48 76 17 20 3 883.06 5.05 77 17 21 4 855.01 6.03 78 18 21 3 883.06 5.06 79 18 22 4 859.86 3.62 80 18 50 32 382.08 38.55 81 19 22 3 880.25 6.46 82 19 23 4 859.97 3.67 83 20 23 3 882.53 5.30 84 20 24 4 864.77 1.28 85 21 24 3 882.53 5.30 86 21 25 4 859.98 3.69 87 21 47 26 381.91 38.50 88 21 50 29 382.13 38.58 89 21 238 217 108.53 1.41 90 22 25 3 862.50 2.43 91 22 26 4 858.90 4.22 92 23 26 3 862.07 2.65 93 23 27 4 849.24 0.97 94 24 27 3 858.14 2.76 95 24 28 4 851.41 2.07 96 24 237 213 110.62 0.36 97 24 238 214 96.35 0.11 98 24 239 215 84.61 0.48 99 24 240 216 82.98 0.32 100 25 28 3 855.47 4.09 101 25 29 4 851.40 2.08 102 25 51 26 382.62 38.83 103 25 54 29 383.83 38.33 104 25 55 30 383.62 38.38 105 26 29 3 859.20 2.24 106 26 30 4 851.39 2.09 107 27 30 3 855.57 0.00 108 27 31 4 851.39 2.09 109 27 240 213 80.25 1.35 110 27 243 216 60.88 1.23 111 28 31 3 856.84 0.63 112 28 41 13 383.68 38.43 113 28 55 27 385.10 38.28 114 28 57 29 385.08 38.29 115 28 60 32 385.03 38.30 116 28 242 214 60.36 0.96 117 29 54 25 385.11 38.28 118 29 55 26 385.09 38.29 119 29 56 27 385.09 38.29 120 29 57 28 385.08 38.29 121 31 57 26 388.59 36.73 122 31 243 212 51.70 0.90 123 31 247 216 51.12 0.77 124 32 247 215 50.31 0.87 125 33 37 4 864.78 0.10 126 33 39 6 495.92 8.83 127 33 57 24 537.28 5.64 128 33 59 26 479.22 0.48 129 33 60 27 479.22 0.48 130 33 246 213 50.23 0.88 131 35 59 24 572.52 0.45 132 35 246 211 50.22 0.92 133 35 247 212 46.61 0.10 134 35 249 214 36.97 0.58 135 35 250 215 30.59 1.56 136 36 59 23 572.55 0.46 137 37 59 22 572.58 0.46 138 37 246 209 40.90 0.26 139 37 249 212 32.84 1.03 140 38 59 21 572.59 0.46 141 38 60 22 572.58 0.46 142 38 61 23 572.55 0.46 143 38 90 52 153.72 17.36 144 39 59 20 572.62 0.47 145 39 60 21 572.57 0.47 146 39 61 22 572.57 0.47 147 39 246 207 41.38 0.02 148 40 61 21 572.58 0.46 149 40 63 23 572.53 0.46 150 40 90 50 154.03 17.48 151 40 124 84 150.87 17.43 152 41 51 10 572.88 0.39 153 41 55 14 572.87 0.41 154 41 60 19 572.67 0.45 155 41 61 20 572.62 0.46 156 41 62 21 572.56 0.46 157 41 63 22 572.54 0.46 158 42 63 21 572.54 0.46 159 42 65 23 572.26 0.57 160 42 92 50 154.04 17.47 161 43 47 4 826.27 1.61 162 43 48 5 696.57 61.38 163 43 51 8 572.94 0.38 164 43 63 20 572.62 0.46 165 43 65 22 572.54 0.46 166 44 47 3 862.99 2.04 167 44 48 4 830.59 0.53 168 44 65 21 572.61 0.46 169 45 48 3 871.66 2.59 170 45 64 19 572.65 0.45 171 45 66 21 572.64 0.45 172 45 78 33 170.66 16.12 173 47 50 3 868.43 4.75 174 47 51 4 856.45 1.24 175 48 51 3 863.28 1.20 176 48 52 4 856.45 1.24 177 48 62 14 572.81 0.40 178 48 63 15 572.78 0.40 179 48 83 35 163.52 17.82 180 48 86 38 163.48 17.80 181 48 88 40 158.33 16.48 182 49 52 3 865.09 3.08 183 49 53 4 856.45 1.24 184 49 86 37 163.48 17.80 185 50 53 3 867.48 1.89 186 50 54 4 848.47 2.60 187 51 54 3 864.06 3.60 188 51 55 4 827.24 2.74 189 51 62 11 572.78 0.40 190 52 86 34 163.46 17.80 191 55 60 5 696.88 61.52 192 55 62 7 572.75 0.44 193 57 60 3 867.05 2.29 194 60 246 186 50.28 0.86 195 61 90 29 153.98 17.45 196 62 88 26 154.25 17.53 197 62 90 28 154.04 17.48 198 63 88 25 154.88 17.51 199 63 90 27 154.04 17.48 200 63 91 28 154.16 17.54 201 64 83 19 163.09 17.90 202 64 88 24 155.34 17.28 203 64 90 26 154.25 17.55 204 64 91 27 154.25 17.55 205 64 92 28 154.24 17.54 206 65 92 27 154.27 17.55 207 66 91 25 154.97 17.48 208 66 92 26 154.81 17.55 209 66 93 27 154.37 17.59 210 66 98 32 154.62 17.57 211 66 112 46 161.33 16.24 212 67 78 11 170.25 15.96 213 67 79 12 170.22 15.94 214 67 80 13 170.22 15.95 215 67 83 16 170.18 15.96 216 67 91 24 156.38 18.09 217 67 93 26 156.29 18.14 218 67 112 45 162.19 15.84 219 68 74 6 446.13 8.51 220 68 77 9 171.01 16.09 221 68 78 10 170.91 16.14 222 68 79 11 170.30 15.90 223 68 80 12 170.28 15.96 224 68 112 44 162.21 15.85 225 69 80 11 170.69 16.12 226 69 81 12 171.02 16.08 227 69 115 46 167.34 15.48 228 70 74 4 925.03 3.81 229 70 79 9 171.01 16.09 230 70 81 11 171.03 16.07 231 71 79 8 171.05 16.07 232 71 81 10 171.06 16.07 233 71 82 11 171.05 16.07 234 74 77 3 864.13 2.18 235 78 82 4 841.99 0.21 236 78 83 5 841.64 0.40 237 79 82 3 868.75 1.28 238 79 83 4 847.93 3.18 239 80 83 3 854.20 4.73 240 80 84 4 847.58 3.35 241 80 91 11 396.79 33.54 242 80 115 35 399.43 32.41 243 80 116 36 399.42 32.41 244 80 121 41 470.45 0.45 245 81 84 3 859.10 1.75 246 81 85 4 847.58 3.35 247 82 85 3 854.52 0.54 248 82 86 4 847.58 3.35 249 83 86 3 860.61 0.62 250 83 87 4 850.83 4.26 251 83 88 5 811.73 4.67 252 83 91 8 529.39 25.05 253 83 121 38 581.17 0.30 254 84 87 3 857.53 0.91 255 84 88 4 822.78 0.59 256 84 119 35 581.16 0.28 257 84 121 37 581.15 0.27 258 88 121 33 655.66 0.15 259 89 121 32 655.63 0.17 260 89 122 33 655.62 0.18 261 90 121 31 655.71 0.18 262 90 122 32 655.63 0.18 263 90 124 34 655.62 0.18 264 90 163 73 655.64 0.18 265 91 116 25 655.85 0.15 266 91 121 30 655.83 0.15 267 91 122 31 655.76 0.18 268 91 123 32 655.67 0.17 269 91 124 33 655.64 0.17 270 92 124 32 655.69 0.19 271 92 126 34 655.62 0.18 272 92 232 140 160.79 20.41 273 93 112 19 655.92 0.17 274 93 113 20 655.85 0.15 275 93 116 23 655.93 0.17 276 93 123 30 655.82 0.15 277 93 124 31 655.77 0.18 278 93 125 32 655.69 0.15 279 93 126 33 655.64 0.17 280 94 99 5 687.07 8.83 281 94 104 10 655.88 0.17 282 94 109 15 655.85 0.15 283 94 126 32 655.70 0.17 284 95 98 3 850.84 4.83 285 95 99 4 837.73 7.70 286 95 126 31 655.76 0.18 287 95 167 72 655.70 0.14 288 95 232 137 161.47 20.09 289 96 99 3 847.94 6.29 290 96 100 4 837.72 7.70 291 96 167 71 655.67 0.12 292 96 169 73 652.85 0.24 293 97 100 3 849.74 1.93 294 97 101 4 819.03 0.35 295 97 102 5 744.58 37.58 296 98 102 4 666.00 0.65 297 98 103 5 660.75 1.70 298 98 104 6 656.29 0.12 299 99 104 5 656.27 0.11 300 99 106 7 656.27 0.12 301 99 109 10 656.28 0.11 302 99 126 27 655.85 0.14 303 99 127 28 655.84 0.14 304 99 146 47 656.12 0.07 305 103 108 5 858.58 2.71 306 104 108 4 853.62 5.20 307 104 109 5 836.30 3.45 308 104 112 8 818.97 2.88 309 105 108 3 866.95 1.46 310 105 109 4 861.74 1.15 311 106 109 3 866.95 1.46 312 106 110 4 861.84 1.09 313 106 145 39 656.32 0.03 314 106 146 40 656.13 0.07 315 106 149 43 656.38 0.02 316 107 110 3 861.84 1.09 317 107 111 4 861.84 1.09 318 107 153 46 658.39 0.85 319 108 111 3 861.75 1.14 320 108 112 4 853.57 5.18 321 109 112 3 863.25 1.92 322 109 113 4 846.62 4.56 323 110 113 3 863.25 1.93 324 110 114 4 852.28 1.73 325 110 149 39 656.47 0.00 326 110 150 40 656.40 0.03 327 110 153 43 658.42 0.87 328 111 114 3 854.11 2.65 329 111 115 4 852.28 1.73 330 111 153 42 658.47 0.84 331 112 115 3 872.03 1.96 332 112 116 4 852.29 1.73 333 113 116 3 867.90 2.63 334 113 117 4 857.09 4.12 335 113 123 10 658.34 0.91 336 113 125 12 656.39 0.01 337 113 150 37 658.37 0.89 338 113 154 41 658.66 0.92 339 113 162 49 658.32 0.92 340 114 117 3 867.62 0.25 341 114 118 4 850.68 0.90 342 114 153 39 658.60 0.89 343 114 154 40 658.59 0.89 344 115 118 3 851.26 1.19 345 115 119 4 849.44 0.28 346 116 119 3 861.49 2.60 347 116 120 4 846.30 1.28 348 116 121 5 812.76 0.41 349 116 123 7 666.93 4.90 350 117 120 3 854.98 5.85 351 117 121 4 821.45 4.74 352 117 123 6 666.95 4.90 353 117 154 37 681.22 7.41 354 117 158 41 681.23 7.41 355 117 161 44 681.09 7.30 356 117 162 45 666.94 4.90 357 121 161 40 795.42 0.78 358 122 160 38 795.49 0.79 359 122 161 39 795.41 0.78 360 122 163 41 795.41 0.79 361 122 207 85 358.59 26.16 362 123 161 38 795.48 0.79 363 123 162 39 795.43 0.79 364 123 163 40 795.42 0.79 365 124 163 39 795.43 0.79 366 124 165 41 795.35 0.79 367 124 209 85 358.20 26.34 368 125 150 25 795.69 0.80 369 125 163 38 795.49 0.79 370 125 164 39 795.43 0.79 371 125 165 40 795.43 0.79 372 126 165 39 795.43 0.79 373 126 167 41 795.38 0.80 374 126 232 106 161.53 20.13 375 127 146 19 795.85 0.77 376 127 147 20 795.84 0.77 377 127 150 23 795.82 0.78 378 127 164 37 795.52 0.79 379 127 165 38 795.48 0.79 380 127 166 39 795.44 0.79 381 127 167 40 795.41 0.80 382 127 190 63 366.85 25.75 383 128 143 15 796.04 0.74 384 128 146 18 796.03 0.75 385 128 166 38 795.46 0.79 386 128 167 39 795.42 0.79 387 128 169 41 795.20 0.83 388 129 133 4 832.33 5.93 389 129 134 5 824.86 2.20 390 129 139 10 796.16 0.74 391 129 142 13 796.15 0.74 392 129 143 14 796.13 0.75 393 129 166 37 795.51 0.79 394 129 169 40 795.32 0.83 395 129 170 41 795.11 0.88 396 130 133 3 859.93 2.94 397 130 134 4 846.48 1.13 398 130 169 39 795.28 0.85 399 130 170 40 795.09 0.85 400 131 134 3 875.94 1.01 401 131 135 4 846.52 1.12 402 131 170 39 795.27 0.86 403 131 174 43 743.35 0.99 404 131 176 45 688.38 2.46 405 132 135 3 873.65 2.27 406 132 136 4 852.36 4.04 407 133 136 3 872.31 2.94 408 133 137 4 852.24 3.98 409 133 138 5 796.46 0.76 410 133 139 6 796.34 0.82 411 133 142 9 796.32 0.82 412 134 137 3 869.30 4.45 413 134 139 5 796.34 0.82 414 134 166 32 795.64 0.78 415 134 170 36 795.26 0.86 416 134 182 48 368.31 25.68 417 135 176 41 745.44 1.97 418 138 141 3 848.64 0.19 419 138 142 4 834.65 4.12 420 139 142 3 874.36 0.18 421 139 143 4 841.42 0.67 422 139 189 50 367.15 25.77 423 140 143 3 874.54 0.09 424 140 144 4 840.40 0.42 425 140 189 49 367.13 25.76 426 141 144 3 867.25 3.56 427 141 145 4 856.55 1.79 428 142 145 3 868.58 2.72 429 142 146 4 867.08 3.47 430 143 146 3 868.63 4.25 431 143 147 4 867.08 3.47 432 143 166 23 795.62 0.79 433 143 189 46 367.00 25.78 434 143 190 47 366.88 25.75 435 144 147 3 869.77 4.96 436 144 148 4 858.09 4.60 437 144 189 45 367.01 25.78 438 144 190 46 366.90 25.76 439 144 193 49 366.83 25.75 440 145 148 3 869.73 4.38 441 145 149 4 860.62 3.33 442 146 149 3 864.12 1.58 443 146 150 4 858.09 4.59 444 147 150 3 863.59 1.84 445 147 151 4 845.78 1.54 446 147 159 12 796.01 0.78 447 147 164 17 795.65 0.80 448 147 193 46 366.62 25.85 449 148 151 3 863.88 2.71 450 148 152 4 849.68 3.06 451 148 193 45 366.63 25.85 452 149 152 3 876.76 3.74 453 150 153 3 879.99 2.58 454 150 154 4 857.41 0.53 455 151 154 3 861.08 2.36 456 151 159 8 796.41 0.74 457 151 193 42 366.61 25.86 458 151 198 47 361.76 25.88 459 154 158 4 849.82 3.03 460 154 159 5 821.27 1.60 461 154 162 8 796.44 0.71 462 155 158 3 870.29 0.62 463 155 159 4 868.88 0.07 464 156 159 3 882.13 1.96 465 156 198 42 361.56 25.98 466 156 199 43 361.56 25.98 467 156 201 45 361.55 25.98 468 156 202 46 360.99 25.88 469 157 160 3 880.96 1.36 470 158 161 3 880.96 1.36 471 159 162 3 879.81 5.43 472 159 164 5 880.14 5.26 473 159 194 35 365.74 25.97 474 159 198 39 361.56 25.98 475 159 202 43 359.49 25.88 476 159 206 47 358.87 26.09 477 162 206 44 358.86 26.10 478 163 207 44 358.58 26.16 479 163 209 46 358.39 26.24 480 164 190 26 366.62 25.85 481 164 206 42 359.04 26.07 482 164 207 43 358.67 26.19 483 164 208 44 358.54 26.16 484 164 209 45 358.39 26.23 485 165 209 44 358.39 26.23 486 165 232 67 161.46 20.09 487 166 186 20 366.85 25.76 488 166 187 21 366.81 25.75 489 166 190 24 366.63 25.85 490 166 208 42 358.71 26.20 491 166 209 43 358.57 26.16 492 166 210 44 358.38 26.23 493 166 211 45 358.12 26.30 494 167 211 44 357.19 26.68 495 168 171 3 873.20 8.18 496 168 172 4 856.77 6.54 497 168 178 10 367.44 25.87 498 168 210 42 358.68 26.19 499 168 211 43 358.41 26.23 500 168 212 44 358.11 26.30 501 169 172 3 849.82 1.83 502 170 174 4 823.35 3.66 503 170 176 6 584.26 51.99 504 170 178 8 368.36 25.66 505 170 182 12 368.27 25.64 506 171 174 3 872.68 1.75 507 171 176 5 387.79 21.21 508 171 178 7 368.29 25.65 509 172 211 39 358.56 26.14 510 172 212 40 358.27 26.23 511 178 182 4 848.48 1.05 512 178 210 32 359.07 26.14 513 179 182 3 859.01 1.12 514 179 183 4 853.84 3.72 515 180 183 3 861.48 0.10 516 180 184 4 858.74 1.28 517 180 221 41 217.40 0.39 518 180 222 42 217.33 0.42 519 180 225 45 165.62 20.46 520 181 184 3 865.61 2.15 521 181 185 4 862.18 0.44 522 182 185 3 869.16 0.38 523 182 186 4 862.18 0.44 524 183 186 3 873.12 1.60 525 183 187 4 850.03 0.23 526 183 210 27 359.07 26.14 527 183 222 39 171.99 23.09 528 184 187 3 864.77 1.81 529 184 188 4 848.26 1.08 530 184 225 41 164.72 20.80 531 184 226 42 164.81 20.76 532 184 227 43 164.60 20.80 533 185 188 3 854.26 1.92 534 185 189 4 840.83 3.14 535 186 189 3 852.60 2.75 536 186 190 4 851.13 3.48 537 187 190 3 863.60 3.16 538 187 191 4 847.63 1.73 539 187 208 21 359.35 26.14 540 187 209 22 359.35 26.14 541 187 210 23 359.30 26.16 542 187 227 40 164.31 20.73 543 187 228 41 164.22 20.68 544 188 191 3 863.75 3.09 545 188 192 4 847.63 1.73 546 188 227 39 164.36 20.72 547 189 192 3 867.54 0.87 548 189 193 4 854.58 1.50 549 190 193 3 865.25 2.02 550 190 194 4 854.58 1.50 551 190 208 18 359.44 26.07 552 191 194 3 858.73 1.25 553 191 195 4 851.76 1.29 554 191 203 12 361.12 25.80 555 191 206 15 359.93 26.03 556 191 208 17 359.57 26.04 557 191 227 36 164.31 20.73 558 191 229 38 164.27 20.73 559 192 195 3 856.51 1.08 560 192 196 4 851.75 1.29 561 193 196 3 859.87 0.60 562 193 197 4 851.73 1.28 563 193 198 5 837.49 2.40 564 194 197 3 871.21 6.11 565 194 198 4 826.49 5.15 566 194 199 5 826.45 5.16 567 194 202 8 396.75 20.92 568 194 203 9 376.17 21.95 569 194 206 12 364.64 27.68 570 195 199 4 811.27 0.24 571 195 200 5 731.00 35.73 572 195 203 8 376.17 21.95 573 198 202 4 849.93 4.37 574 199 202 3 855.49 3.25 575 199 203 4 848.74 3.77 576 200 203 3 857.19 3.79 577 200 204 4 848.74 3.77 578 201 204 3 877.85 3.33 579 201 205 4 856.71 4.04 580 202 205 3 877.84 3.33 581 202 206 4 831.49 4.80 582 203 206 3 874.65 4.93 583 206 229 23 163.73 20.62 584 207 229 22 163.72 20.62 585 208 227 19 164.12 20.67 586 208 228 20 163.91 20.64 587 208 229 21 163.72 20.61 588 209 229 20 163.74 20.59 589 209 230 21 162.97 20.24 590 209 231 22 162.02 20.06 591 209 232 23 161.99 20.08 592 210 214 4 864.24 5.70 593 210 222 12 164.16 20.75 594 210 228 18 164.10 20.65 595 210 230 20 163.44 20.45 596 210 231 21 162.43 20.23 597 210 232 22 162.34 20.20 598 211 232 21 161.63 20.10 599 213 218 5 837.27 5.29 600 214 218 4 837.26 5.29 601 214 219 5 575.97 113.47 602 214 222 8 409.88 30.46 603 214 231 17 163.24 20.25 604 214 233 19 162.72 20.09 605 214 236 22 161.55 20.08 606 214 245 31 100.73 26.14 607 215 218 3 857.35 0.43 608 215 219 4 812.26 3.93 609 215 236 21 161.72 20.01 610 215 241 26 102.67 26.36 611 215 245 30 100.75 26.14 612 216 219 3 861.03 3.95 613 216 241 25 105.89 28.02 614 216 244 28 101.00 26.17 615 216 245 29 100.98 26.26 616 218 221 3 882.19 5.42 617 218 222 4 849.73 0.93 618 219 222 3 880.06 4.05 619 219 223 4 852.50 2.19 620 219 231 12 164.69 20.67 621 219 245 26 100.58 26.22 622 219 248 29 37.67 0.51 623 220 223 3 857.00 2.71 624 220 224 4 852.51 2.20 625 220 248 28 37.62 0.53 626 220 250 30 37.58 0.51 627 221 224 3 852.81 2.05 628 221 225 4 852.51 2.20 629 222 225 3 863.99 1.94 630 222 228 6 282.16 36.66 631 222 231 9 164.71 20.66 632 223 228 5 215.68 3.42 633 223 229 6 215.67 3.42 634 223 230 7 214.36 4.04 635 223 231 8 164.85 20.70 636 223 248 25 37.44 0.45 637 223 249 26 26.68 0.25 638 225 228 3 840.62 11.22 639 228 231 3 1025.44 4.32 640 231 245 14 94.72 24.93 641 231 249 18 26.45 0.37 642 233 236 3 898.70 4.67 643 233 237 4 821.81 1.75 644 233 242 9 139.21 6.90 645 233 245 12 98.42 25.24 646 234 237 3 871.74 0.41 647 235 238 3 885.88 6.66 648 235 239 4 859.34 0.78 649 236 239 3 862.17 1.35 650 236 241 5 430.08 14.84 651 236 242 6 325.91 57.01 652 236 245 9 283.39 77.52 653 237 242 5 325.69 56.90 654 239 242 3 876.52 3.52 655 240 243 3 888.97 9.74 656 241 244 3 863.78 3.12 657 241 245 4 837.68 9.52 658 242 245 3 838.95 8.88 659 242 246 4 843.88 6.41 660 243 246 3 843.88 6.41 661 243 247 4 840.56 4.75 662 244 247 3 842.94 3.56 663 244 248 4 849.78 0.13 664 245 248 3 858.12 2.65 665 245 249 4 853.49 1.83 666 246 249 3 881.06 5.09 667 247 250 3 886.12 2.79