BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 116.45 0.88 2 3 228 225 115.73 1.23 3 3 229 226 112.59 0.62 4 4 204 200 142.31 11.65 5 4 205 201 142.31 11.65 6 4 206 202 142.31 11.64 7 4 207 203 141.81 11.45 8 4 229 225 116.97 0.90 9 5 36 31 677.75 0.20 10 5 37 32 677.75 0.20 11 5 38 33 677.74 0.20 12 5 207 202 142.68 11.81 13 6 36 30 677.79 0.21 14 6 37 31 677.76 0.20 15 6 38 32 677.75 0.20 16 6 223 217 117.53 0.77 17 6 229 223 117.53 0.77 18 6 249 243 22.70 0.12 19 7 37 30 677.78 0.20 20 7 38 31 677.75 0.19 21 7 40 33 677.74 0.20 22 7 90 83 238.56 57.48 23 7 163 156 227.18 53.79 24 7 207 200 149.62 15.12 25 7 209 202 143.34 12.11 26 7 229 222 118.00 0.58 27 7 230 223 117.56 0.77 28 7 231 224 117.53 0.77 29 8 37 29 677.83 0.19 30 8 38 30 677.80 0.20 31 8 39 31 677.75 0.19 32 8 40 32 677.75 0.19 33 8 231 223 117.56 0.78 34 8 242 234 55.78 0.73 35 8 246 238 44.70 1.32 36 9 40 31 677.75 0.19 37 9 124 115 236.93 56.88 38 9 231 222 118.03 0.58 39 9 232 223 117.56 0.78 40 9 233 224 117.55 0.78 41 9 242 233 55.80 0.73 42 10 40 30 677.81 0.20 43 10 41 31 677.76 0.20 44 10 42 32 677.75 0.19 45 10 233 223 117.56 0.78 46 10 237 227 86.81 0.09 47 10 242 232 55.80 0.73 48 11 42 31 677.77 0.19 49 11 65 54 463.00 0.20 50 11 95 84 237.96 57.34 51 11 232 221 119.31 0.12 52 11 233 222 117.98 0.57 53 11 234 223 117.56 0.78 54 11 237 226 91.12 0.90 55 12 15 3 881.85 1.17 56 12 21 9 678.89 0.05 57 12 24 12 678.86 0.06 58 12 25 13 678.85 0.07 59 12 28 16 678.84 0.07 60 12 41 29 677.83 0.20 61 12 42 30 677.79 0.20 62 12 43 31 677.76 0.18 63 12 65 53 463.56 0.15 64 12 237 225 114.36 1.35 65 12 238 226 90.95 0.97 66 13 44 31 677.78 0.19 67 13 65 52 463.30 0.09 68 13 95 82 238.49 57.50 69 13 97 84 238.40 57.49 70 13 234 221 119.33 0.12 71 14 44 30 677.80 0.19 72 15 44 29 677.81 0.19 73 15 238 223 117.47 0.74 74 16 21 5 765.12 40.19 75 16 47 31 677.45 0.07 76 17 20 3 872.77 2.04 77 17 21 4 855.14 4.54 78 17 47 30 677.43 0.07 79 18 21 3 872.09 1.70 80 18 22 4 861.65 1.29 81 18 50 32 654.21 9.47 82 19 22 3 868.12 3.68 83 19 23 4 861.65 1.28 84 20 23 3 867.08 3.18 85 20 24 4 861.65 1.28 86 21 24 3 866.25 3.58 87 21 25 4 853.45 3.75 88 21 47 26 677.22 0.12 89 21 50 29 604.87 3.16 90 21 238 217 108.91 4.03 91 22 25 3 861.72 1.30 92 22 26 4 849.83 0.39 93 22 50 28 599.12 3.65 94 22 54 32 465.77 0.24 95 23 26 3 863.34 0.49 96 23 27 4 863.96 0.80 97 24 27 3 863.06 1.25 98 24 28 4 857.19 3.29 99 24 237 213 113.50 1.92 100 24 238 214 99.23 0.72 101 24 239 215 91.87 1.52 102 24 240 216 89.81 1.51 103 25 28 3 862.48 5.93 104 25 29 4 854.73 4.53 105 25 51 26 514.70 24.19 106 25 54 29 465.67 0.21 107 25 55 30 465.34 0.06 108 26 29 3 863.11 1.22 109 26 30 4 852.12 2.71 110 26 54 28 465.64 0.18 111 27 30 3 864.83 1.43 112 27 31 4 852.11 2.71 113 27 240 213 83.62 0.74 114 27 243 216 69.19 3.09 115 28 31 3 871.93 1.53 116 28 41 13 677.85 0.18 117 28 55 27 465.58 0.18 118 28 57 29 464.40 0.12 119 28 60 32 464.17 0.21 120 28 242 214 66.91 4.23 121 29 54 25 465.63 0.18 122 29 55 26 465.34 0.06 123 29 56 27 465.13 0.07 124 29 57 28 464.39 0.12 125 31 57 26 464.38 0.12 126 31 243 212 55.41 0.53 127 33 37 4 862.96 1.71 128 33 39 6 679.27 0.51 129 33 57 24 464.20 0.18 130 33 59 26 463.97 0.20 131 33 60 27 464.03 0.23 132 33 246 213 49.05 0.62 133 35 59 24 463.88 0.20 134 35 246 211 47.97 0.35 135 35 247 212 44.90 1.27 136 35 249 214 37.88 0.32 137 35 250 215 25.70 0.74 138 36 59 23 463.86 0.20 139 37 246 209 44.86 1.25 140 37 249 212 27.19 0.65 141 38 59 21 463.79 0.20 142 38 61 23 463.77 0.20 143 38 90 52 238.56 57.47 144 39 59 20 463.82 0.19 145 39 60 21 463.78 0.20 146 39 61 22 463.77 0.20 147 39 246 207 44.85 1.25 148 40 61 21 463.78 0.20 149 40 63 23 463.65 0.18 150 40 90 50 238.94 57.65 151 41 51 10 465.44 0.18 152 41 55 14 464.20 0.18 153 41 60 19 463.88 0.20 154 41 61 20 463.81 0.20 155 41 62 21 463.76 0.19 156 41 63 22 463.65 0.18 157 42 63 21 463.66 0.18 158 42 65 23 463.52 0.18 159 42 92 50 238.93 57.66 160 43 47 4 773.07 38.56 161 43 48 5 679.79 0.46 162 43 51 8 514.67 24.21 163 43 63 20 463.79 0.20 164 43 65 22 463.66 0.17 165 44 47 3 867.62 3.62 166 44 48 4 850.28 0.04 167 44 65 21 463.75 0.18 168 45 48 3 868.56 3.15 169 45 64 19 463.79 0.20 170 45 66 21 463.75 0.18 171 45 78 33 289.98 34.22 172 45 83 38 289.48 34.47 173 47 50 3 865.44 1.40 174 47 51 4 841.16 0.66 175 48 51 3 864.86 1.10 176 48 52 4 843.85 2.00 177 48 63 15 464.02 0.14 178 48 83 35 288.56 34.89 179 48 86 38 288.47 34.87 180 48 88 40 278.08 39.20 181 49 52 3 869.49 2.81 182 49 53 4 853.09 11.02 183 49 86 37 288.35 34.84 184 50 53 3 869.50 2.82 185 50 54 4 845.36 7.15 186 51 54 3 869.49 2.81 187 51 55 4 832.11 0.53 188 51 62 11 464.09 0.15 189 52 62 10 464.13 0.15 190 52 86 34 288.31 34.83 191 55 60 5 466.75 1.05 192 55 62 7 464.20 0.19 193 57 60 3 870.71 3.98 194 60 242 182 57.54 0.14 195 60 246 186 47.58 0.11 196 61 90 29 238.58 57.47 197 62 88 26 240.54 56.98 198 62 90 28 238.94 57.65 199 63 88 25 241.92 56.91 200 63 90 27 238.94 57.65 201 63 91 28 238.97 57.66 202 64 83 19 288.20 34.79 203 64 88 24 242.38 57.00 204 64 90 26 241.15 56.72 205 64 91 27 240.52 56.96 206 64 92 28 241.09 56.69 207 65 92 27 241.38 56.66 208 66 83 17 289.93 34.24 209 66 91 25 242.39 56.94 210 66 92 26 242.19 56.86 211 66 93 27 242.20 56.84 212 66 98 32 242.66 56.61 213 66 112 46 276.53 39.93 214 67 78 11 290.00 34.23 215 67 79 12 290.00 34.23 216 67 80 13 289.99 34.22 217 67 83 16 289.99 34.23 218 67 91 24 246.52 54.92 219 67 93 26 246.46 54.88 220 67 112 45 277.03 39.69 221 68 74 6 398.81 32.71 222 68 77 9 290.13 34.24 223 68 78 10 290.12 34.24 224 68 79 11 290.01 34.23 225 68 80 12 290.07 34.25 226 68 112 44 278.44 38.99 227 69 80 11 290.11 34.24 228 69 81 12 290.13 34.26 229 69 115 46 288.32 34.79 230 70 74 4 912.24 2.48 231 70 79 9 290.16 34.22 232 71 74 3 986.16 2.35 233 71 79 8 290.19 34.21 234 71 81 10 290.24 34.23 235 71 82 11 290.24 34.23 236 74 77 3 875.09 4.92 237 78 82 4 853.91 2.38 238 78 83 5 853.81 2.33 239 79 82 3 867.72 4.51 240 79 83 4 853.93 2.38 241 80 83 3 858.05 1.67 242 80 84 4 853.88 2.38 243 80 91 11 365.78 1.65 244 80 115 35 366.78 2.21 245 80 116 36 365.71 1.69 246 80 121 41 419.86 26.84 247 81 84 3 863.34 1.70 248 81 85 4 853.88 2.38 249 82 85 3 860.76 0.28 250 82 86 4 859.40 0.40 251 83 86 3 859.41 0.40 252 83 87 4 859.40 0.40 253 83 88 5 755.42 35.33 254 83 91 8 424.39 22.23 255 83 121 38 481.97 1.55 256 84 87 3 856.19 1.21 257 84 88 4 831.85 2.89 258 84 119 35 485.16 0.05 259 84 121 37 484.80 0.12 260 88 121 33 552.10 1.94 261 89 121 32 552.07 1.95 262 89 122 33 552.06 1.95 263 90 121 31 552.12 1.94 264 90 122 32 552.08 1.95 265 90 124 34 552.06 1.94 266 90 163 73 552.08 1.95 267 91 116 25 552.27 1.94 268 91 121 30 552.24 1.94 269 91 122 31 552.16 1.93 270 91 123 32 552.08 1.95 271 91 124 33 552.08 1.95 272 92 95 3 918.68 4.06 273 92 124 32 552.08 1.95 274 92 126 34 552.04 1.94 275 92 232 140 122.98 0.82 276 93 112 19 552.44 1.94 277 93 113 20 552.37 1.95 278 93 116 23 552.44 1.94 279 93 123 30 552.25 1.94 280 93 124 31 552.18 1.94 281 93 125 32 552.08 1.94 282 93 126 33 552.06 1.94 283 94 99 5 823.47 2.56 284 94 104 10 552.38 1.94 285 94 109 15 552.32 1.91 286 94 126 32 552.06 1.93 287 95 98 3 871.13 0.58 288 95 99 4 856.84 4.16 289 95 126 31 552.08 1.94 290 95 167 72 552.04 1.93 291 95 232 137 123.44 0.64 292 96 99 3 856.85 4.16 293 96 100 4 844.70 0.98 294 96 167 71 552.04 1.93 295 96 169 73 548.90 1.32 296 97 100 3 864.89 2.56 297 97 101 4 841.43 2.61 298 97 102 5 827.35 4.44 299 98 101 3 866.41 1.80 300 98 102 4 827.31 4.44 301 98 103 5 827.31 4.44 302 98 104 6 692.09 62.58 303 99 104 5 553.73 2.18 304 99 106 7 553.73 2.18 305 99 109 10 553.66 2.21 306 99 126 27 552.37 1.98 307 99 127 28 552.36 1.99 308 99 146 47 552.49 2.00 309 103 108 5 867.31 8.85 310 104 108 4 848.44 0.04 311 104 109 5 839.78 4.29 312 104 112 8 825.46 2.69 313 105 108 3 873.12 5.94 314 105 109 4 851.04 1.28 315 106 109 3 875.27 4.87 316 106 110 4 851.14 1.23 317 106 145 39 552.85 1.95 318 106 146 40 552.55 2.00 319 106 149 43 552.88 1.96 320 107 110 3 875.27 4.87 321 107 111 4 859.66 2.94 322 108 111 3 871.58 6.88 323 108 112 4 853.72 0.06 324 109 112 3 868.34 1.41 325 109 113 4 851.78 1.03 326 109 150 41 552.93 1.94 327 110 113 3 864.33 0.92 328 110 114 4 851.80 1.04 329 110 149 39 552.93 1.95 330 110 150 40 552.92 1.95 331 110 153 43 553.12 1.96 332 111 114 3 862.98 0.24 333 111 115 4 858.34 2.08 334 112 115 3 866.81 2.16 335 112 116 4 852.06 0.92 336 113 116 3 863.67 3.73 337 113 117 4 852.06 0.92 338 113 123 10 553.04 1.95 339 113 125 12 552.81 1.92 340 113 150 37 553.06 1.95 341 113 154 41 553.75 1.97 342 114 117 3 863.66 3.73 343 114 118 4 847.11 1.56 344 114 153 39 553.63 1.91 345 114 154 40 553.71 1.95 346 115 118 3 866.34 2.39 347 115 119 4 843.38 0.30 348 116 119 3 871.54 0.21 349 116 120 4 845.30 1.27 350 116 121 5 820.27 4.47 351 116 123 7 556.09 3.15 352 117 120 3 866.33 2.39 353 117 121 4 831.13 1.69 354 117 123 6 626.54 32.05 355 117 154 37 692.90 0.64 356 117 158 41 628.14 31.35 357 117 161 44 627.75 31.55 358 117 162 45 627.73 31.54 359 121 161 40 796.93 2.46 360 122 160 38 797.01 2.46 361 122 161 39 796.93 2.46 362 122 163 41 796.91 2.46 363 122 207 85 245.74 25.84 364 123 161 38 796.99 2.47 365 123 162 39 796.95 2.46 366 123 163 40 796.94 2.46 367 124 163 39 796.94 2.46 368 124 165 41 796.90 2.46 369 124 209 85 245.19 26.11 370 124 232 108 123.00 0.83 371 125 150 25 797.12 2.47 372 125 163 38 796.99 2.46 373 125 164 39 796.94 2.46 374 125 165 40 796.94 2.46 375 126 165 39 796.94 2.46 376 126 167 41 796.90 2.47 377 126 232 106 123.42 0.65 378 127 143 16 797.36 2.44 379 127 146 19 797.27 2.48 380 127 147 20 797.26 2.46 381 127 150 23 797.16 2.47 382 127 164 37 797.05 2.46 383 127 165 38 796.99 2.46 384 127 166 39 796.96 2.47 385 127 167 40 796.93 2.46 386 127 190 63 302.08 21.40 387 128 142 14 797.39 2.44 388 128 143 15 797.37 2.44 389 128 146 18 797.36 2.44 390 128 166 38 796.99 2.46 391 128 167 39 796.93 2.46 392 128 169 41 796.69 2.47 393 129 133 4 838.58 0.06 394 129 134 5 836.61 1.05 395 129 139 10 797.43 2.43 396 129 142 13 797.40 2.43 397 129 143 14 797.40 2.43 398 129 166 37 797.00 2.46 399 129 169 40 796.88 2.48 400 129 170 41 796.69 2.48 401 130 133 3 861.88 0.38 402 130 134 4 847.50 4.52 403 130 169 39 796.79 2.52 404 130 170 40 796.65 2.50 405 131 134 3 853.06 1.75 406 131 135 4 847.49 4.50 407 131 170 39 796.78 2.52 408 131 174 43 737.01 2.60 409 131 176 45 721.46 3.84 410 132 135 3 864.31 3.71 411 132 136 4 849.99 3.27 412 133 136 3 857.95 1.30 413 133 137 4 847.49 4.51 414 133 138 5 797.76 2.43 415 133 139 6 797.98 2.54 416 133 142 9 797.65 2.44 417 134 137 3 846.39 5.06 418 134 139 5 797.98 2.54 419 134 166 32 797.09 2.46 420 134 170 36 796.89 2.48 421 134 185 51 302.70 21.37 422 135 176 41 755.05 4.06 423 138 141 3 868.81 1.88 424 138 142 4 842.26 1.28 425 139 142 3 870.73 0.92 426 139 143 4 844.62 2.44 427 139 189 50 302.36 21.33 428 140 143 3 868.77 0.07 429 140 144 4 840.45 2.18 430 140 189 49 302.34 21.34 431 141 145 4 850.59 4.64 432 142 145 3 869.24 7.54 433 142 146 4 869.23 7.54 434 143 146 3 869.24 7.54 435 143 147 4 868.78 7.77 436 143 166 23 797.05 2.46 437 143 189 46 302.28 21.32 438 143 190 47 302.29 21.32 439 144 147 3 863.65 1.88 440 144 148 4 856.48 1.61 441 144 189 45 302.28 21.32 442 144 190 46 302.28 21.32 443 144 193 49 301.81 21.54 444 145 148 3 860.52 0.36 445 145 149 4 856.50 1.62 446 146 149 3 874.19 2.45 447 146 150 4 858.81 1.92 448 147 150 3 866.37 5.71 449 147 151 4 858.82 1.92 450 147 159 12 797.52 2.62 451 147 164 17 797.05 2.46 452 147 190 43 302.17 21.35 453 147 193 46 301.68 21.47 454 147 194 47 301.61 21.47 455 148 151 3 860.54 2.77 456 148 152 4 858.82 1.92 457 148 193 45 301.68 21.47 458 149 152 3 869.66 0.52 459 149 153 4 860.96 2.57 460 150 153 3 884.82 1.67 461 150 154 4 860.55 2.77 462 151 154 3 871.52 0.82 463 151 159 8 797.81 2.48 464 151 193 42 301.68 21.47 465 151 198 47 268.42 15.49 466 154 158 4 835.19 3.64 467 154 159 5 848.60 3.06 468 154 162 8 797.82 2.47 469 155 158 3 863.41 0.66 470 155 159 4 864.95 1.43 471 156 159 3 876.26 4.01 472 156 198 42 266.22 16.50 473 156 201 45 251.05 24.07 474 156 202 46 246.55 25.94 475 157 160 3 886.60 1.16 476 158 161 3 889.00 3.58 477 158 162 4 861.85 2.98 478 159 162 3 864.56 1.55 479 159 164 5 864.95 1.35 480 159 194 35 299.39 21.07 481 159 198 39 266.22 16.51 482 159 202 43 246.50 25.96 483 159 206 47 246.07 25.88 484 162 206 44 245.96 25.93 485 163 207 44 245.71 25.86 486 163 209 46 245.20 26.11 487 164 190 26 301.70 21.51 488 164 206 42 246.05 25.96 489 164 207 43 245.96 25.95 490 164 208 44 245.25 26.08 491 164 209 45 245.20 26.11 492 165 209 44 245.18 26.11 493 165 232 67 123.42 0.65 494 166 186 20 302.03 21.43 495 166 187 21 302.03 21.43 496 166 190 24 301.79 21.53 497 166 209 43 245.26 26.08 498 166 210 44 245.18 26.09 499 166 211 45 245.14 26.10 500 167 172 5 850.36 8.39 501 167 211 44 244.91 26.00 502 168 171 3 881.47 5.33 503 168 172 4 867.51 0.18 504 168 178 10 302.52 21.33 505 168 210 42 245.31 26.05 506 168 211 43 245.22 26.07 507 168 212 44 244.75 26.09 508 169 172 3 867.91 2.46 509 169 174 5 865.08 1.03 510 170 174 4 851.09 8.03 511 170 176 6 706.16 3.42 512 170 178 8 321.10 29.59 513 170 182 12 302.72 21.38 514 171 174 3 867.91 2.45 515 171 176 5 632.23 30.39 516 171 178 7 302.70 21.37 517 172 212 40 244.69 26.13 518 178 182 4 840.32 2.75 519 178 210 32 245.98 26.20 520 179 182 3 865.38 3.01 521 179 183 4 861.25 0.95 522 180 183 3 858.86 2.15 523 180 184 4 858.55 2.30 524 180 221 41 150.01 11.21 525 180 222 42 149.92 11.18 526 180 225 45 143.33 8.13 527 181 184 3 874.16 2.03 528 181 185 4 858.55 2.30 529 182 185 3 862.01 4.04 530 182 186 4 858.52 2.29 531 183 186 3 864.59 2.76 532 183 187 4 853.46 0.23 533 183 210 27 246.25 26.05 534 183 222 39 145.22 8.97 535 184 187 3 856.56 0.86 536 184 188 4 853.02 0.01 537 184 225 41 133.31 5.04 538 184 226 42 133.31 5.03 539 184 227 43 133.25 5.04 540 185 188 3 866.48 1.65 541 185 189 4 853.61 0.28 542 186 189 3 854.97 0.40 543 186 190 4 853.20 0.48 544 187 190 3 854.97 0.41 545 187 191 4 847.08 2.27 546 187 208 21 246.35 26.02 547 187 209 22 246.34 26.03 548 187 210 23 246.29 26.04 549 187 227 40 133.03 5.08 550 187 228 41 132.11 4.63 551 188 191 3 861.45 4.51 552 188 192 4 838.04 2.35 553 188 227 39 133.12 5.07 554 189 192 3 870.29 0.09 555 189 193 4 852.36 0.36 556 190 193 3 867.12 5.24 557 190 194 4 852.36 0.36 558 190 208 18 246.37 26.01 559 191 194 3 867.55 0.65 560 191 195 4 841.54 2.19 561 191 203 12 247.15 25.99 562 191 206 15 246.91 26.04 563 191 208 17 246.39 26.00 564 191 227 36 133.07 5.07 565 191 229 38 133.06 5.07 566 192 195 3 871.45 0.85 567 192 196 4 841.55 2.19 568 193 196 3 865.19 3.15 569 193 197 4 838.66 0.74 570 193 198 5 838.66 0.74 571 194 197 3 865.19 3.14 572 194 198 4 836.12 0.53 573 194 199 5 815.21 7.77 574 194 202 8 249.16 25.22 575 194 203 9 247.36 26.03 576 194 206 12 246.85 26.07 577 195 199 4 606.62 96.41 578 195 200 5 255.37 22.13 579 195 203 8 247.41 26.03 580 198 202 4 845.07 1.03 581 199 202 3 850.43 1.44 582 199 203 4 845.07 1.03 583 200 203 3 838.10 2.46 584 200 204 4 835.51 3.77 585 201 204 3 865.71 5.17 586 201 205 4 845.10 1.03 587 202 205 3 871.02 7.70 588 202 206 4 830.01 1.02 589 203 206 3 863.35 4.00 590 206 229 23 131.25 4.34 591 207 229 22 130.94 4.21 592 208 227 19 131.36 4.34 593 208 228 20 131.26 4.33 594 208 229 21 131.01 4.21 595 209 229 20 130.97 4.20 596 209 230 21 126.26 1.96 597 209 231 22 124.08 0.89 598 209 232 23 124.06 0.90 599 210 214 4 883.61 2.50 600 210 222 12 140.15 8.62 601 210 228 18 131.35 4.29 602 210 230 20 130.32 3.87 603 210 231 21 124.08 0.89 604 210 232 22 124.08 0.89 605 211 232 21 123.88 0.80 606 213 218 5 854.05 0.41 607 214 218 4 838.00 7.62 608 214 219 5 533.32 130.05 609 214 222 8 202.69 1.17 610 214 231 17 129.05 3.19 611 214 233 19 124.21 0.84 612 214 236 22 123.92 0.84 613 214 245 31 54.49 0.68 614 215 218 3 881.43 4.37 615 215 219 4 838.00 7.62 616 215 236 21 123.95 0.83 617 215 241 26 60.52 1.64 618 215 245 30 55.11 0.62 619 216 241 25 56.16 0.54 620 216 244 28 55.32 0.54 621 216 245 29 54.91 0.52 622 218 221 3 884.57 6.42 623 218 222 4 847.10 2.40 624 219 222 3 874.16 11.62 625 219 223 4 844.91 1.29 626 219 231 12 133.19 5.00 627 219 245 26 51.20 0.41 628 219 248 29 42.91 2.25 629 220 223 3 857.66 1.88 630 220 224 4 839.40 1.47 631 220 248 28 41.28 3.04 632 220 250 30 41.27 3.04 633 221 224 3 868.93 3.76 634 221 225 4 844.38 1.02 635 222 225 3 871.13 2.66 636 222 228 6 178.27 24.90 637 222 231 9 133.97 5.08 638 223 228 5 216.82 46.25 639 223 229 6 137.01 6.58 640 223 230 7 136.28 6.24 641 223 231 8 134.58 5.39 642 223 248 25 41.24 3.04 643 223 249 26 33.44 0.83 644 225 228 3 860.21 3.02 645 228 231 3 1051.23 2.98 646 230 249 19 22.46 0.01 647 231 245 14 47.84 0.40 648 231 249 18 25.26 1.23 649 233 236 3 900.95 1.61 650 233 237 4 846.35 1.10 651 233 242 9 107.94 26.82 652 233 245 12 51.50 0.56 653 234 237 3 881.45 0.46 654 235 238 3 881.06 0.66 655 235 239 4 877.41 2.14 656 236 239 3 859.56 1.59 657 236 241 5 361.30 6.03 658 236 242 6 187.92 9.88 659 236 245 9 185.67 11.01 660 237 242 5 299.92 41.05 661 239 242 3 873.77 1.72 662 240 243 3 881.35 7.82 663 241 244 3 868.50 1.40 664 241 245 4 863.85 3.72 665 242 245 3 871.05 2.68 666 242 246 4 854.00 1.21 667 243 246 3 860.59 2.55 668 243 247 4 853.52 0.97 669 244 247 3 874.15 1.41 670 244 248 4 850.80 2.33 671 245 248 3 853.97 0.76 672 245 249 4 866.08 5.28 673 246 249 3 872.89 4.83 674 247 250 3 896.44 0.45