BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 229 227 54.47 1.12 2 3 222 219 84.76 0.88 3 3 223 220 84.51 0.82 4 3 224 221 85.05 0.52 5 4 177 173 242.08 11.53 6 4 178 174 243.12 11.47 7 4 221 217 89.13 1.24 8 4 222 218 84.82 0.86 9 4 223 219 88.02 0.75 10 4 224 220 88.67 1.08 11 4 226 222 87.81 0.86 12 5 219 214 94.36 3.21 13 5 220 215 92.78 3.00 14 5 221 216 90.59 1.96 15 6 169 163 246.62 9.87 16 6 177 171 247.20 9.67 17 6 183 177 247.21 9.64 18 6 185 179 247.22 9.59 19 6 219 213 94.39 3.21 20 6 220 214 93.79 3.50 21 7 45 38 248.83 9.77 22 7 50 43 248.46 9.86 23 7 52 45 248.09 9.89 24 7 183 176 247.30 9.64 25 7 184 177 247.20 9.60 26 7 185 178 247.22 9.59 27 7 217 210 100.79 2.44 28 7 218 211 100.56 2.39 29 7 219 212 94.39 3.21 30 7 235 228 74.73 0.28 31 8 185 177 247.22 9.59 32 8 192 184 227.02 0.48 33 8 195 187 214.94 1.24 34 8 196 188 214.22 0.92 35 8 217 209 100.85 2.43 36 8 218 210 100.61 2.37 37 9 41 32 249.27 9.62 38 9 66 57 248.92 9.60 39 9 70 61 249.19 9.60 40 9 184 175 248.50 9.69 41 9 185 176 248.00 9.62 42 9 186 177 247.59 9.40 43 9 187 178 247.59 9.39 44 9 216 207 115.53 4.01 45 9 217 208 100.85 2.43 46 9 218 209 100.74 2.41 47 10 187 177 247.60 9.39 48 10 188 178 247.59 9.39 49 10 189 179 247.50 9.35 50 10 192 182 227.54 0.63 51 10 212 202 130.19 3.44 52 10 213 203 129.81 3.62 53 10 214 204 128.69 3.47 54 10 215 205 128.57 3.50 55 10 248 238 29.80 0.09 56 11 30 19 249.82 9.36 57 11 62 51 248.66 9.64 58 11 63 52 248.65 9.65 59 11 66 55 248.80 9.67 60 11 187 176 248.40 9.53 61 11 188 177 247.93 9.29 62 11 214 203 128.75 3.50 63 12 30 18 249.97 9.44 64 12 34 22 249.69 9.54 65 12 37 25 249.65 9.56 66 12 214 202 129.44 3.62 67 13 17 4 835.05 4.61 68 13 214 201 130.11 3.50 69 14 17 3 848.67 0.11 70 14 18 4 843.58 0.35 71 14 31 17 250.86 9.44 72 15 18 3 851.83 1.47 73 15 19 4 843.57 0.35 74 15 31 16 325.83 38.12 75 16 19 3 843.58 0.35 76 16 20 4 836.51 3.89 77 16 21 5 736.46 35.94 78 16 22 6 684.91 10.17 79 16 23 7 587.13 4.96 80 16 26 10 500.92 37.41 81 16 28 12 373.88 24.84 82 16 96 80 335.35 1.26 83 16 97 81 335.35 1.26 84 16 132 116 377.68 9.12 85 17 20 3 817.50 1.57 86 17 21 4 817.49 1.56 87 18 33 15 413.84 2.44 88 19 99 80 412.25 2.17 89 20 99 79 412.35 2.17 90 21 97 76 413.23 2.52 91 21 98 77 412.54 2.17 92 21 99 78 412.36 2.16 93 21 102 81 412.65 2.23 94 22 92 70 412.35 2.18 95 22 96 74 413.24 2.50 96 22 97 75 413.31 2.50 97 23 26 3 833.99 3.29 98 23 28 5 419.53 3.64 99 23 62 39 413.94 2.48 100 23 96 73 413.43 2.48 101 24 62 38 414.03 2.48 102 24 89 65 413.55 2.48 103 24 90 66 413.49 2.48 104 24 91 67 413.48 2.48 105 24 92 68 413.45 2.48 106 24 96 72 413.47 2.48 107 25 60 35 413.79 2.42 108 25 85 60 413.64 2.47 109 25 88 63 413.63 2.47 110 25 89 64 413.61 2.48 111 25 90 65 413.59 2.48 112 25 91 66 413.49 2.48 113 25 136 111 413.64 2.47 114 25 142 117 413.81 2.45 115 25 153 128 413.55 2.48 116 26 62 36 414.20 2.52 117 26 96 70 413.51 2.45 118 26 132 106 413.76 2.45 119 26 135 109 413.83 2.43 120 27 60 33 413.93 2.47 121 27 61 34 414.10 2.51 122 27 62 35 414.18 2.51 123 27 141 114 413.96 2.46 124 28 62 34 414.20 2.52 125 29 61 32 414.22 2.52 126 30 34 4 824.59 1.27 127 30 35 5 814.15 4.05 128 30 62 32 414.25 2.52 129 30 65 35 414.45 2.53 130 30 66 36 414.46 2.53 131 31 34 3 847.48 0.09 132 31 35 4 835.80 3.14 133 32 35 3 839.55 1.31 134 32 36 4 833.61 4.28 135 33 36 3 844.72 1.28 136 33 37 4 835.83 3.15 137 33 247 214 45.38 0.06 138 34 37 3 840.46 0.86 139 34 38 4 833.68 4.24 140 35 38 3 858.33 4.95 141 35 39 4 833.66 4.23 142 35 69 34 415.44 2.18 143 36 39 3 854.37 1.64 144 36 40 4 826.81 0.82 145 37 40 3 853.89 1.87 146 37 41 4 826.78 0.83 147 37 214 177 129.43 3.62 148 37 215 178 128.13 3.29 149 38 41 3 840.43 3.55 150 38 42 4 836.22 5.65 151 38 66 28 415.42 2.16 152 38 69 31 415.68 2.21 153 38 70 32 415.69 2.21 154 39 42 3 836.23 5.65 155 39 43 4 838.44 4.54 156 39 69 30 415.67 2.21 157 39 73 34 416.90 2.15 158 40 43 3 845.12 5.63 159 40 44 4 833.33 4.20 160 41 44 3 835.55 3.10 161 41 45 4 833.34 4.20 162 41 70 29 416.37 2.05 163 41 215 174 124.38 3.87 164 41 216 175 124.36 3.88 165 41 217 176 119.89 2.02 166 42 45 3 840.68 0.54 167 42 46 4 833.33 4.21 168 42 70 28 415.91 2.28 169 42 73 31 419.19 1.80 170 43 46 3 837.78 1.98 171 43 47 4 820.20 2.31 172 43 73 30 419.28 1.85 173 44 47 3 833.27 4.24 174 44 48 4 820.65 2.08 175 44 217 173 115.65 3.97 176 44 235 191 76.25 0.30 177 44 236 192 74.70 0.24 178 45 48 3 821.97 1.42 179 45 49 4 820.64 2.08 180 45 50 5 806.44 6.32 181 45 52 7 811.72 3.67 182 45 70 25 420.47 1.89 183 45 74 29 523.26 46.07 184 45 217 172 111.92 3.10 185 46 49 3 821.95 1.41 186 46 73 27 423.94 3.59 187 46 74 28 423.94 3.59 188 48 235 187 75.45 0.17 189 50 74 24 793.43 0.12 190 50 76 26 793.50 0.10 191 50 235 185 75.41 0.18 192 51 76 25 793.50 0.10 193 51 182 131 307.51 2.43 194 52 74 22 793.62 0.12 195 52 76 24 793.56 0.12 196 52 79 27 793.42 0.14 197 52 182 130 307.50 2.43 198 52 183 131 307.50 2.42 199 52 184 132 307.42 2.42 200 53 79 26 793.43 0.14 201 53 80 27 793.44 0.13 202 53 181 128 307.56 2.42 203 53 182 129 307.53 2.42 204 54 79 25 793.47 0.12 205 54 80 26 793.43 0.14 206 54 172 118 368.44 1.84 207 54 176 122 358.64 1.42 208 54 181 127 307.55 2.42 209 54 182 128 307.51 2.41 210 54 183 129 304.45 2.21 211 54 184 130 304.24 2.21 212 55 67 12 793.74 0.14 213 55 71 16 793.68 0.12 214 55 79 24 793.55 0.12 215 55 80 25 793.50 0.13 216 55 81 26 793.41 0.14 217 55 82 27 793.41 0.14 218 55 184 129 301.17 0.73 219 56 82 26 793.45 0.13 220 56 87 31 726.26 4.45 221 56 168 112 385.19 0.57 222 56 172 116 361.19 2.17 223 56 173 117 360.84 2.27 224 56 176 120 359.51 1.80 225 56 183 127 301.30 0.73 226 56 184 128 301.18 0.73 227 56 185 129 301.10 0.73 228 56 186 130 301.10 0.73 229 57 63 6 793.82 0.14 230 57 64 7 793.76 0.15 231 57 67 10 793.78 0.15 232 57 81 24 793.58 0.14 233 57 82 25 793.52 0.14 234 57 83 26 793.44 0.13 235 57 84 27 793.40 0.13 236 57 87 30 726.19 4.48 237 57 186 129 301.06 0.73 238 58 63 5 793.90 0.12 239 58 64 6 793.87 0.12 240 58 83 25 793.48 0.12 241 58 84 26 793.43 0.12 242 58 87 29 726.27 4.44 243 58 88 30 726.15 4.49 244 58 188 130 301.03 0.74 245 59 64 5 806.00 3.14 246 59 83 24 793.53 0.13 247 59 84 25 793.47 0.11 248 59 85 26 793.39 0.13 249 59 88 29 759.29 4.74 250 59 141 82 566.56 2.52 251 59 142 83 566.62 2.56 252 60 63 3 850.89 4.73 253 60 64 4 821.86 0.79 254 60 88 28 770.02 0.64 255 60 89 29 760.20 4.28 256 60 141 81 567.18 2.44 257 60 142 82 567.17 2.44 258 60 188 128 300.97 0.73 259 61 64 3 832.75 0.63 260 61 65 4 816.81 0.08 261 61 141 80 567.18 2.44 262 62 66 4 828.33 2.44 263 62 89 27 726.29 4.44 264 62 188 126 300.97 0.72 265 63 66 3 844.51 5.25 266 63 67 4 843.43 1.02 267 63 186 123 301.05 0.73 268 63 188 125 300.97 0.72 269 64 67 3 843.43 1.02 270 64 68 4 839.37 3.08 271 64 83 19 793.53 0.13 272 65 68 3 849.18 2.58 273 65 69 4 839.37 3.08 274 66 69 3 850.03 2.16 275 66 70 4 834.23 5.66 276 66 186 120 301.06 0.72 277 67 70 3 848.88 1.61 278 67 71 4 832.04 6.75 279 67 186 119 301.06 0.72 280 68 71 3 838.62 3.51 281 68 72 4 832.05 6.75 282 69 72 3 851.10 2.19 283 69 73 4 825.09 1.81 284 70 73 3 835.57 3.43 285 70 74 4 823.02 0.78 286 70 184 114 301.22 0.70 287 71 74 3 828.37 5.05 288 71 75 4 821.00 1.72 289 71 76 5 793.93 0.08 290 71 77 6 793.83 0.13 291 71 79 8 793.83 0.13 292 71 81 10 793.65 0.09 293 72 75 3 815.39 1.14 294 72 77 5 793.83 0.13 295 76 79 3 846.62 0.58 296 82 168 86 390.24 1.35 297 82 172 90 364.11 0.70 298 84 87 3 847.50 1.50 299 84 168 84 385.05 0.52 300 85 88 3 785.98 30.49 301 85 142 57 566.20 2.69 302 85 145 60 566.31 2.67 303 85 146 61 566.35 2.65 304 85 147 62 566.29 2.67 305 85 152 67 558.10 1.39 306 85 153 68 543.56 0.72 307 85 164 79 391.74 1.30 308 86 146 60 566.68 2.50 309 86 147 61 566.35 2.64 310 86 164 78 391.72 1.31 311 86 165 79 391.25 1.35 312 86 167 81 389.44 0.96 313 86 168 82 380.74 0.63 314 87 164 77 391.74 1.30 315 87 165 78 391.28 1.34 316 87 168 81 377.87 0.81 317 87 173 86 360.89 2.29 318 87 188 101 301.04 0.73 319 87 191 104 300.81 0.77 320 88 142 54 567.20 2.44 321 88 164 76 391.73 1.31 322 88 188 100 300.94 0.74 323 88 191 103 300.75 0.75 324 89 164 75 391.74 1.31 325 89 188 99 300.88 0.76 326 89 189 100 300.88 0.76 327 89 190 101 300.85 0.76 328 89 191 102 300.73 0.75 329 90 115 25 557.67 1.95 330 90 123 33 550.15 2.40 331 90 156 66 523.50 0.28 332 90 157 67 519.45 1.39 333 90 161 71 391.84 1.28 334 90 164 74 391.77 1.29 335 90 190 100 300.69 0.77 336 91 96 5 820.10 5.26 337 91 126 35 566.83 2.31 338 91 137 46 567.30 2.43 339 91 153 62 565.95 2.54 340 91 154 63 563.31 1.42 341 91 155 64 530.50 1.26 342 91 156 65 525.61 0.60 343 92 95 3 858.48 5.53 344 92 96 4 817.99 6.30 345 92 123 31 561.88 0.72 346 92 190 98 300.70 0.77 347 93 96 3 864.18 2.68 348 93 124 31 566.78 2.30 349 93 125 32 566.79 2.32 350 93 126 33 566.82 2.31 351 93 154 61 566.54 2.31 352 93 155 62 552.83 1.16 353 94 98 4 866.14 1.70 354 94 124 30 566.88 2.29 355 94 125 31 566.91 2.28 356 94 126 32 566.98 2.31 357 94 130 36 567.31 2.40 358 95 98 3 860.31 1.05 359 95 103 8 576.01 0.45 360 95 107 12 567.31 2.44 361 95 115 20 567.05 2.39 362 95 123 28 566.79 2.25 363 95 124 29 567.05 2.32 364 96 126 30 567.20 2.39 365 96 130 34 567.39 2.39 366 96 132 36 567.32 2.43 367 96 136 40 567.56 2.33 368 98 102 4 828.15 11.02 369 98 103 5 814.81 0.68 370 98 106 8 710.33 4.45 371 99 102 3 845.38 7.20 372 99 103 4 840.18 5.05 373 100 103 3 849.37 3.98 374 100 104 4 842.56 3.87 375 101 104 3 859.98 6.28 376 101 105 4 846.07 5.62 377 102 105 3 854.23 2.77 378 102 106 4 843.70 6.82 379 103 106 3 860.13 0.18 380 103 107 4 830.25 0.09 381 103 112 9 589.92 11.02 382 104 107 3 849.50 0.41 383 104 108 4 677.48 0.76 384 104 109 5 603.99 6.49 385 104 112 8 593.66 11.64 386 107 111 4 839.50 4.65 387 107 112 5 824.33 5.11 388 107 115 8 706.34 6.09 389 107 124 17 567.77 2.35 390 108 111 3 840.59 4.11 391 108 112 4 833.33 7.73 392 109 112 3 851.08 1.12 393 109 113 4 837.68 5.58 394 110 113 3 853.50 2.29 395 111 114 3 880.05 2.44 396 111 115 4 853.52 2.28 397 112 115 3 856.50 0.80 398 112 116 4 853.50 2.29 399 113 116 3 872.34 3.18 400 113 117 4 833.77 7.42 401 114 117 3 844.83 3.52 402 114 118 4 807.12 4.48 403 114 121 7 568.82 1.97 404 115 121 6 568.81 1.97 405 115 122 7 568.81 1.97 406 115 123 8 568.80 1.97 407 115 124 9 568.86 1.99 408 117 208 91 189.08 0.56 409 118 121 3 850.15 0.86 410 118 208 90 189.55 0.34 411 119 157 38 568.91 1.99 412 119 158 39 568.87 1.97 413 119 159 40 568.70 1.89 414 119 162 43 568.51 1.79 415 119 197 78 288.14 0.76 416 119 204 85 193.99 1.80 417 119 207 88 189.61 0.31 418 119 208 89 189.40 0.27 419 120 157 37 568.93 2.00 420 120 159 39 568.87 1.96 421 121 157 36 569.00 1.98 422 122 155 33 569.26 2.03 423 122 156 34 569.15 2.04 424 122 157 35 569.01 1.99 425 122 160 38 567.37 1.16 426 123 155 32 569.37 2.07 427 123 156 33 569.25 2.02 428 123 157 34 569.21 2.01 429 124 155 31 569.50 2.02 430 125 154 29 569.53 2.04 431 125 155 30 569.51 2.04 432 126 130 4 804.12 0.07 433 126 133 7 570.62 1.98 434 126 136 10 570.61 1.98 435 126 137 11 570.61 1.98 436 126 154 28 569.55 2.04 437 127 130 3 818.58 6.28 438 127 133 6 570.62 1.99 439 128 133 5 570.62 1.99 440 130 133 3 822.04 0.63 441 132 135 3 848.68 2.40 442 132 136 4 841.66 5.90 443 133 136 3 851.64 0.91 444 133 137 4 836.10 8.67 445 133 154 21 569.69 2.08 446 134 137 3 851.64 0.91 447 134 138 4 778.13 18.89 448 135 139 4 792.40 22.84 449 136 139 3 847.23 2.93 450 136 142 6 832.15 2.96 451 137 149 12 569.96 2.06 452 137 153 16 569.90 2.04 453 137 154 17 569.82 2.02 454 138 143 5 848.91 0.92 455 139 142 3 868.12 1.26 456 139 143 4 857.50 1.27 457 140 143 3 857.50 1.27 458 140 144 4 857.63 1.21 459 141 144 3 871.62 0.54 460 141 145 4 857.63 1.21 461 142 145 3 867.41 1.56 462 142 149 7 570.12 2.06 463 142 152 10 570.09 2.06 464 143 149 6 570.12 2.06 465 145 152 7 641.35 1.86 466 147 151 4 834.43 1.60 467 147 152 5 827.68 4.97 468 148 151 3 854.83 1.69 469 148 152 4 843.00 1.88 470 149 152 3 863.62 1.72 471 149 154 5 864.38 3.10 472 150 154 4 880.90 0.50 473 153 156 3 900.20 0.95 474 156 160 4 799.92 2.35 475 156 161 5 747.89 28.06 476 156 164 8 744.28 29.87 477 157 160 3 817.48 11.13 478 157 161 4 822.47 4.04 479 158 161 3 844.39 3.32 480 158 162 4 832.71 9.17 481 158 190 32 300.86 0.75 482 158 193 35 297.68 0.59 483 158 194 36 294.08 0.79 484 158 207 49 189.52 0.29 485 158 208 50 185.59 0.63 486 159 162 3 851.02 0.01 487 159 163 4 825.39 5.51 488 160 163 3 834.34 1.04 489 160 164 4 825.39 5.51 490 161 164 3 847.30 5.19 491 161 165 4 823.55 3.54 492 161 190 29 300.89 0.76 493 161 191 30 300.85 0.75 494 161 194 33 294.18 0.78 495 162 165 3 826.44 4.99 496 162 166 4 824.36 5.65 497 162 194 32 294.24 0.75 498 162 197 35 293.89 0.82 499 162 198 36 287.23 0.31 500 163 167 4 841.43 0.32 501 164 167 3 872.02 2.18 502 165 168 3 858.97 4.35 503 165 169 4 840.50 0.14 504 165 191 26 300.93 0.74 505 165 194 29 300.54 0.81 506 165 195 30 296.44 0.00 507 166 169 3 835.84 2.19 508 166 170 4 835.84 2.19 509 166 198 32 293.97 0.81 510 167 170 3 859.35 3.24 511 167 171 4 834.74 2.62 512 168 172 4 764.65 24.52 513 168 173 5 715.02 0.29 514 169 173 4 715.01 0.29 515 169 174 5 715.03 0.29 516 169 175 6 824.04 8.21 517 169 177 8 715.00 0.30 518 169 195 26 300.74 0.77 519 169 198 29 294.21 0.79 520 169 199 30 294.07 0.78 521 169 222 53 93.87 3.48 522 169 226 57 89.97 1.85 523 170 174 4 826.11 7.17 524 170 175 5 840.66 0.10 525 171 175 4 843.05 0.76 526 172 175 3 870.22 1.60 527 172 176 4 843.82 0.38 528 173 176 3 856.38 2.59 529 173 177 4 841.73 0.54 530 173 183 10 304.30 2.14 531 173 185 12 301.35 0.74 532 174 177 3 843.84 0.52 533 174 178 4 841.73 0.54 534 174 226 52 89.88 1.90 535 175 178 3 854.73 2.29 536 175 179 4 845.34 2.41 537 176 179 3 847.32 1.42 538 176 180 4 845.28 2.44 539 176 181 5 694.70 2.44 540 176 183 7 505.60 35.29 541 177 180 3 843.66 0.41 542 177 183 6 373.69 30.68 543 177 222 45 91.45 2.40 544 177 226 49 89.88 1.89 545 178 224 46 89.98 1.92 546 178 226 48 89.77 1.94 547 185 195 10 300.69 0.73 548 187 191 4 767.38 21.93 549 187 192 5 758.80 25.20 550 188 191 3 833.67 1.80 551 188 192 4 823.76 6.25 552 189 192 3 872.37 0.96 553 189 193 4 830.14 4.44 554 189 212 23 130.35 3.50 555 190 193 3 830.14 4.44 556 190 194 4 830.14 4.44 557 191 194 3 845.56 2.49 558 191 195 4 840.58 0.93 559 192 195 3 861.76 5.51 560 192 196 4 838.62 1.90 561 192 212 20 130.22 3.43 562 193 196 3 861.76 5.51 563 193 197 4 838.62 1.90 564 193 207 14 168.78 1.00 565 193 210 17 131.44 3.05 566 193 212 19 130.31 3.48 567 194 197 3 846.46 1.89 568 194 198 4 835.73 3.35 569 195 198 3 845.11 1.97 570 195 199 4 854.69 9.04 571 196 199 3 858.61 7.08 572 196 200 4 832.60 2.00 573 196 202 6 749.76 29.82 574 196 212 16 130.22 3.43 575 196 220 24 100.59 2.37 576 196 232 36 83.18 0.79 577 197 200 3 860.07 6.34 578 197 201 4 752.24 29.67 579 197 202 5 683.31 0.35 580 197 203 6 683.31 0.35 581 197 204 7 683.14 0.43 582 197 207 10 170.93 0.97 583 198 201 3 837.56 3.45 584 199 220 21 100.40 2.35 585 199 222 23 94.28 3.69 586 199 226 27 91.29 2.39 587 199 227 28 89.84 1.92 588 199 228 29 89.79 1.92 589 200 220 20 100.52 2.40 590 200 228 28 89.84 1.91 591 200 232 32 84.37 1.55 592 202 206 4 846.04 1.78 593 202 207 5 830.24 2.40 594 202 210 8 641.25 20.57 595 203 206 3 853.85 2.54 596 203 207 4 846.03 1.78 597 204 207 3 869.30 5.19 598 204 208 4 869.29 5.19 599 205 208 3 881.70 1.00 600 205 209 4 862.24 2.81 601 206 209 3 860.74 2.06 602 206 210 4 852.51 3.20 603 207 210 3 861.13 1.88 604 210 232 22 86.84 0.11 605 211 234 23 84.56 0.74 606 211 245 34 31.57 0.03 607 211 248 37 29.09 0.24 608 212 218 6 124.06 3.29 609 212 248 36 28.94 0.17 610 213 216 3 858.81 0.04 611 213 234 21 83.03 1.43 612 213 244 31 33.72 1.02 613 213 245 32 33.33 0.83 614 213 248 35 29.12 0.09 615 214 244 30 33.73 1.02 616 214 248 34 29.44 0.24 617 216 234 18 78.78 1.03 618 216 235 19 77.74 0.75 619 216 244 28 34.45 0.66 620 217 234 17 76.38 0.38 621 217 235 18 75.64 0.07 622 218 232 14 82.47 1.32 623 218 233 15 77.25 0.48 624 218 234 16 75.67 0.06 625 219 232 13 79.94 0.14 626 219 233 14 75.78 0.09 627 219 235 16 74.64 0.29 628 220 228 8 87.59 0.76 629 220 232 12 75.77 0.10 630 221 228 7 89.45 1.79 631 221 229 8 86.59 0.43 632 221 233 12 75.70 0.05 633 222 226 4 108.20 11.05 634 222 227 5 89.88 1.89 635 222 228 6 88.68 2.43 636 222 229 7 88.43 2.30 637 223 226 3 835.02 0.10 638 223 227 4 357.32 113.50 639 223 229 6 88.69 2.44 640 234 241 7 44.19 2.06 641 234 245 11 33.31 0.88 642 236 241 5 774.07 26.00 643 237 240 3 849.15 2.72 644 237 241 4 822.99 1.79 645 238 241 3 833.43 2.17 646 238 242 4 825.30 0.63 647 239 242 3 847.66 3.87 648 239 243 4 825.30 0.63 649 240 243 3 836.60 0.62 650 240 244 4 832.85 2.57 651 241 244 3 836.65 0.68 652 241 245 4 832.22 2.89 653 242 245 3 832.23 2.90 654 242 246 4 832.20 2.90 655 243 246 3 834.33 1.83 656 243 247 4 832.37 2.81 657 244 247 3 835.16 4.20 658 244 248 4 834.84 4.04 659 245 248 3 842.66 0.46 660 245 249 4 825.71 0.53 661 246 249 3 837.88 2.52