BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 671 675 4 824.55 1.38 2 671 840 169 26.34 0.26 3 672 675 3 838.99 3.14 4 672 676 4 844.58 0.35 5 673 676 3 853.58 4.84 6 673 677 4 844.58 0.35 7 673 723 50 29.10 0.58 8 673 806 133 27.41 0.18 9 673 834 161 27.41 0.18 10 673 837 164 27.41 0.18 11 674 677 3 844.59 0.34 12 674 678 4 824.51 1.35 13 674 802 128 28.09 0.07 14 674 840 166 28.28 0.17 15 674 841 167 28.09 0.07 16 675 678 3 850.85 3.23 17 675 679 4 824.64 1.28 18 676 679 3 844.37 2.14 19 676 680 4 833.65 3.22 20 676 721 45 34.02 0.40 21 676 723 47 33.21 0.24 22 677 680 3 856.91 0.79 23 677 681 4 829.40 3.87 24 677 722 45 33.68 0.40 25 677 723 46 33.67 0.40 26 677 805 128 32.95 0.12 27 677 806 129 32.95 0.12 28 677 809 132 32.95 0.11 29 678 681 3 856.91 0.80 30 678 805 127 33.02 0.21 31 680 718 38 34.18 0.35 32 680 721 41 34.08 0.40 33 680 722 42 34.08 0.40 34 681 718 37 34.23 0.33 35 681 804 123 34.09 0.36 36 681 805 124 33.99 0.41 37 681 808 127 34.08 0.36 38 682 714 32 35.69 0.98 39 682 718 36 34.27 0.34 40 684 766 82 86.69 0.35 41 685 707 22 86.71 0.34 42 685 710 25 84.08 0.41 43 685 711 26 78.14 1.29 44 685 714 29 79.23 0.57 45 685 765 80 86.68 0.35 46 685 766 81 86.77 0.33 47 686 707 21 86.76 0.33 48 686 710 24 86.78 0.32 49 686 765 79 86.74 0.33 50 686 766 80 86.78 0.34 51 686 767 81 86.81 0.35 52 687 703 16 86.85 0.34 53 687 706 19 86.85 0.34 54 687 707 20 86.77 0.32 55 687 710 23 86.79 0.32 56 687 765 78 86.79 0.34 57 687 767 80 86.86 0.34 58 687 768 81 86.81 0.35 59 688 710 22 86.87 0.34 60 688 767 79 86.95 0.32 61 689 693 4 888.46 4.20 62 689 699 10 87.06 0.35 63 689 700 11 87.00 0.34 64 689 703 14 86.95 0.32 65 689 767 78 86.98 0.32 66 689 768 79 86.95 0.33 67 689 774 85 155.56 33.92 68 690 693 3 870.38 4.83 69 691 767 76 155.28 33.78 70 691 774 83 162.94 32.54 71 693 699 6 772.71 12.71 72 695 700 5 812.58 7.23 73 695 773 78 177.63 25.32 74 695 774 79 177.00 25.63 75 695 777 82 177.65 25.32 76 696 699 3 843.94 0.41 77 696 700 4 835.10 4.01 78 696 777 81 177.71 25.29 79 697 700 3 840.82 3.38 80 697 701 4 824.26 1.40 81 697 777 80 177.71 25.29 82 697 778 81 177.72 25.28 83 697 779 82 177.72 25.28 84 697 880 183 172.27 13.86 85 697 887 190 173.05 13.32 86 697 889 192 173.00 13.30 87 698 701 3 857.77 6.51 88 698 702 4 827.34 0.14 89 698 887 189 173.05 13.32 90 698 888 190 173.03 13.31 91 698 889 191 172.99 13.29 92 699 702 3 839.52 3.50 93 699 703 4 827.33 0.14 94 700 703 3 846.13 5.00 95 700 704 4 831.80 0.01 96 700 768 68 189.78 21.58 97 700 770 70 187.77 20.66 98 700 774 74 187.70 20.59 99 700 777 77 187.71 20.59 100 700 778 78 187.62 20.59 101 701 704 3 842.84 5.53 102 701 705 4 824.07 3.87 103 701 778 77 187.72 20.59 104 701 876 175 179.90 16.64 105 701 880 179 173.33 13.35 106 701 889 188 175.26 12.23 107 702 705 3 835.27 1.72 108 702 706 4 827.37 2.24 109 702 889 187 175.27 12.23 110 702 890 188 175.25 12.22 111 703 706 3 832.36 0.32 112 703 707 4 824.09 3.88 113 703 768 65 192.09 20.43 114 704 707 3 838.45 3.29 115 704 708 4 838.44 3.29 116 704 764 60 192.14 20.46 117 704 768 64 192.16 20.43 118 704 770 66 192.12 20.44 119 704 780 76 190.15 19.57 120 705 708 3 838.45 3.29 121 705 709 4 838.45 3.29 122 705 889 184 181.78 9.09 123 705 890 185 181.68 9.08 124 705 891 186 181.33 9.20 125 705 892 187 181.72 8.96 126 705 895 190 169.41 6.66 127 706 709 3 838.34 3.35 128 706 710 4 835.41 4.80 129 706 892 186 182.27 8.72 130 707 710 3 843.55 6.37 131 707 711 4 837.99 3.52 132 707 764 57 195.57 18.96 133 707 765 58 195.59 18.96 134 708 711 3 846.94 0.97 135 708 712 4 837.98 3.50 136 708 745 37 195.62 18.93 137 708 895 187 178.12 4.57 138 709 712 3 846.93 0.97 139 709 713 4 846.93 0.97 140 709 892 183 184.68 7.57 141 709 893 184 185.58 7.09 142 709 894 185 184.59 7.49 143 709 895 186 183.07 6.87 144 710 713 3 852.53 1.83 145 710 714 4 842.72 3.08 146 711 714 3 846.69 1.07 147 711 715 4 830.43 1.49 148 711 745 34 201.50 16.08 149 711 752 41 201.88 16.18 150 712 715 3 856.49 1.31 151 712 716 4 830.39 1.48 152 712 741 29 201.55 16.02 153 712 745 33 201.80 16.14 154 712 894 182 185.74 7.02 155 712 895 183 185.69 7.00 156 712 898 186 183.83 6.16 157 713 716 3 858.02 4.74 158 713 717 4 836.22 1.45 159 713 894 181 185.81 7.00 160 714 717 3 836.80 1.75 161 714 718 4 823.36 4.98 162 715 718 3 836.23 1.44 163 715 719 4 822.19 4.35 164 715 737 22 203.18 15.72 165 715 744 29 202.97 15.63 166 715 745 30 202.97 15.63 167 716 719 3 846.16 2.02 168 716 720 4 822.37 4.25 169 716 737 21 205.64 14.30 170 717 720 3 849.26 0.48 171 717 721 4 837.45 6.29 172 718 721 3 864.89 1.98 173 718 722 4 837.77 6.13 174 718 744 26 207.86 14.22 175 718 808 90 208.71 14.64 176 718 809 91 208.83 14.71 177 718 812 94 208.71 14.64 178 719 722 3 837.21 3.79 179 719 725 6 649.94 6.33 180 719 737 18 207.84 14.21 181 719 812 93 207.82 14.21 182 720 725 5 649.94 6.33 183 720 726 6 552.15 16.05 184 722 725 3 834.74 7.03 185 722 809 87 233.11 26.30 186 722 812 90 230.28 23.71 187 722 834 112 268.98 43.01 188 723 830 107 270.23 43.60 189 723 834 111 269.07 43.05 190 724 727 3 861.20 2.88 191 724 826 102 286.26 51.40 192 724 830 106 270.23 43.59 193 725 728 3 861.62 2.66 194 725 733 8 577.26 3.70 195 725 736 11 384.31 100.17 196 725 737 12 384.41 100.12 197 725 812 87 269.33 42.93 198 725 813 88 269.35 42.94 199 725 830 105 274.66 45.59 200 726 733 7 692.74 15.34 201 727 826 99 287.56 51.73 202 728 732 4 824.62 9.98 203 728 733 5 801.11 0.34 204 728 736 8 780.87 5.50 205 728 813 85 287.69 51.66 206 728 826 98 287.56 51.73 207 728 827 99 287.56 51.73 208 728 830 102 287.60 51.71 209 729 732 3 828.31 8.15 210 729 733 4 824.96 9.82 211 729 822 93 308.97 44.73 212 730 733 3 847.58 1.48 213 730 734 4 832.02 6.34 214 731 734 3 860.25 1.32 215 731 735 4 832.01 6.33 216 731 822 91 317.26 42.82 217 732 735 3 836.23 4.24 218 732 736 4 836.23 4.23 219 732 821 89 317.15 42.83 220 732 822 90 317.29 42.83 221 732 823 91 322.12 45.24 222 733 736 3 863.64 4.08 223 733 737 4 842.93 0.89 224 734 737 3 858.06 3.21 225 734 738 4 832.44 2.37 226 735 738 3 847.80 8.35 227 735 739 4 836.08 4.19 228 735 821 86 327.45 44.01 229 736 739 3 846.93 3.27 230 736 740 4 830.38 7.03 231 736 812 76 321.25 40.91 232 736 821 85 334.48 40.52 233 736 827 91 327.48 44.00 234 737 740 3 855.57 2.74 235 737 741 4 820.06 1.88 236 737 812 75 332.10 41.58 237 738 741 3 870.76 0.83 238 738 898 160 227.28 21.86 239 738 902 164 222.90 21.97 240 739 815 76 334.56 40.51 241 739 817 78 339.25 39.51 242 739 902 163 222.93 22.00 243 739 905 166 222.16 22.36 244 739 906 167 217.86 21.20 245 739 911 172 204.44 24.94 246 740 743 3 844.32 7.08 247 740 744 4 827.39 1.50 248 740 811 71 334.57 40.45 249 740 812 72 334.57 40.45 250 740 815 75 334.74 40.42 251 741 744 3 831.48 0.62 252 741 745 4 837.28 6.44 253 741 895 154 240.62 20.46 254 741 898 157 232.50 24.46 255 741 899 158 231.84 24.75 256 742 745 3 850.75 0.28 257 742 746 4 837.28 6.44 258 742 870 128 341.82 33.99 259 742 873 131 328.11 25.79 260 742 874 132 317.74 20.61 261 742 877 135 318.15 17.71 262 743 746 3 850.75 0.28 263 743 747 4 837.29 6.45 264 743 811 68 349.67 37.94 265 743 866 123 344.60 35.43 266 743 870 127 341.92 34.01 267 744 747 3 849.18 0.51 268 744 748 4 849.18 0.51 269 744 808 64 349.75 37.95 270 744 811 67 349.70 37.93 271 744 812 68 349.70 37.93 272 745 748 3 849.18 0.51 273 745 749 4 834.22 7.98 274 746 749 3 846.50 0.85 275 746 750 4 829.55 7.62 276 746 787 41 370.86 43.01 277 746 790 44 355.36 35.26 278 746 869 123 354.92 35.22 279 746 873 127 350.45 32.97 280 747 750 3 838.46 3.17 281 747 751 4 851.03 3.12 282 747 790 43 370.81 42.98 283 747 794 47 369.84 42.40 284 747 804 57 369.30 42.18 285 747 807 60 356.97 36.06 286 747 808 61 356.92 36.04 287 747 811 64 356.46 35.91 288 747 862 115 355.21 35.34 289 747 866 119 355.02 35.27 290 748 751 3 851.07 3.10 291 748 752 4 820.53 3.10 292 748 804 56 369.37 42.27 293 748 808 60 356.88 36.11 294 749 752 3 832.71 2.98 295 749 753 4 820.54 3.10 296 749 762 13 372.83 42.47 297 749 763 14 372.83 42.48 298 749 764 15 372.73 42.45 299 749 787 38 371.26 42.87 300 750 753 3 838.79 0.06 301 750 754 4 820.55 3.10 302 750 791 41 370.96 42.86 303 750 794 44 371.13 42.80 304 751 754 3 834.93 1.99 305 751 755 4 822.24 2.27 306 751 794 43 371.14 42.79 307 751 799 48 370.97 42.70 308 751 800 49 370.98 42.71 309 751 801 50 370.98 42.70 310 751 804 53 369.86 42.19 311 752 755 3 840.68 1.13 312 752 756 4 817.45 0.13 313 752 763 11 372.88 42.47 314 752 764 12 372.95 42.43 315 753 756 3 829.55 4.44 316 753 757 4 804.24 0.84 317 753 758 5 773.63 0.76 318 753 759 6 518.08 1.89 319 753 761 8 380.44 45.90 320 753 762 9 373.10 42.36 321 753 763 10 372.88 42.47 322 753 791 38 371.80 42.61 323 754 758 4 683.74 45.56 324 754 759 5 518.09 1.89 325 754 791 37 371.80 42.60 326 754 794 40 371.75 42.58 327 754 795 41 371.69 42.52 328 756 763 7 373.32 42.36 329 757 760 3 840.34 0.85 330 757 761 4 657.04 31.12 331 757 763 6 380.45 45.91 332 757 769 12 373.25 42.44 333 759 788 29 371.94 42.61 334 759 791 32 371.87 42.60 335 759 792 33 371.88 42.60 336 761 769 8 373.26 42.47 337 761 770 9 373.13 42.48 338 761 771 10 373.08 42.47 339 762 769 7 486.75 14.28 340 762 770 8 373.12 42.49 341 762 775 13 373.12 42.49 342 762 784 22 372.00 42.64 343 762 787 25 371.95 42.63 344 762 788 26 371.91 42.62 345 763 766 3 891.51 1.40 346 763 769 6 502.74 6.28 347 764 768 4 791.05 0.38 348 764 770 6 683.71 17.80 349 764 787 23 371.91 42.62 350 765 768 3 829.87 1.60 351 765 769 4 791.14 0.43 352 766 769 3 879.72 3.85 353 768 774 6 809.26 4.49 354 769 774 5 819.19 0.28 355 770 774 4 809.32 4.51 356 770 775 5 809.26 4.49 357 770 778 8 789.23 5.33 358 770 784 14 381.58 47.31 359 771 774 3 863.58 5.02 360 771 775 4 820.78 0.54 361 772 775 3 829.93 0.36 362 772 776 4 824.47 2.37 363 772 781 9 556.13 12.19 364 773 776 3 842.30 6.54 365 773 777 4 824.47 2.37 366 774 777 3 826.60 1.30 367 774 778 4 829.28 4.80 368 775 778 3 847.24 2.60 369 775 780 5 809.07 4.62 370 775 781 6 560.93 16.79 371 775 784 9 508.51 5.98 372 776 779 3 831.31 3.79 373 776 781 5 629.09 5.94 374 778 876 98 428.05 10.35 375 779 876 97 429.78 11.21 376 780 783 3 834.22 1.96 377 780 784 4 826.56 1.46 378 780 787 7 812.35 0.69 379 780 873 93 430.21 11.43 380 780 876 96 426.32 9.48 381 781 784 3 829.82 0.17 382 781 785 4 826.59 1.47 383 782 785 3 838.35 0.29 384 782 786 4 831.38 0.61 385 783 786 3 841.23 0.58 386 783 787 4 831.38 0.62 387 783 872 89 430.51 11.28 388 783 873 90 430.50 11.27 389 783 876 93 431.65 10.27 390 784 787 3 844.31 3.26 391 784 788 4 823.33 4.65 392 785 788 3 836.19 7.32 393 785 789 4 825.14 5.16 394 786 789 3 840.51 3.95 395 786 790 4 827.50 5.50 396 786 868 82 433.48 10.29 397 786 869 83 433.31 10.20 398 786 872 86 433.40 10.13 399 787 790 3 831.02 3.75 400 787 791 4 831.05 3.75 401 787 869 82 433.39 10.16 402 787 873 86 433.06 9.96 403 788 791 3 831.06 3.75 404 788 792 4 831.27 3.86 405 789 792 3 838.50 0.24 406 789 793 4 838.50 0.24 407 790 793 3 839.47 0.24 408 790 794 4 838.50 0.24 409 790 862 72 433.68 10.31 410 790 865 75 433.65 10.29 411 790 866 76 433.66 10.29 412 790 869 79 433.50 10.14 413 791 794 3 850.38 1.41 414 792 795 3 840.89 1.15 415 792 796 4 821.51 6.59 416 793 796 3 842.57 2.25 417 793 797 4 821.03 6.83 418 793 862 69 434.07 10.42 419 794 798 4 717.89 6.79 420 794 799 5 541.96 1.88 421 794 804 10 523.32 7.44 422 794 862 68 433.81 10.28 423 795 798 3 831.48 3.29 424 797 855 58 477.74 8.52 425 797 858 61 474.73 7.57 426 797 862 65 435.10 10.68 427 798 855 57 477.93 8.52 428 799 803 4 820.39 2.71 429 799 804 5 802.20 2.56 430 799 807 8 778.13 7.33 431 799 855 56 477.94 8.52 432 799 859 60 477.85 8.54 433 799 862 63 477.78 8.53 434 800 803 3 837.39 2.44 435 800 804 4 826.52 0.35 436 801 804 3 835.68 1.57 437 801 805 4 826.52 0.35 438 802 805 3 838.95 0.06 439 802 806 4 828.13 0.46 440 802 841 39 482.89 9.27 441 803 806 3 836.32 1.18 442 803 807 4 825.95 1.55 443 803 841 38 482.85 9.26 444 803 855 52 482.63 9.18 445 803 859 56 481.73 9.27 446 804 807 3 830.75 3.96 447 804 808 4 825.95 1.57 448 805 808 3 845.84 4.57 449 805 809 4 834.74 2.00 450 806 809 3 837.73 0.51 451 806 810 4 837.34 1.02 452 806 834 28 483.50 9.42 453 806 837 31 483.23 9.38 454 806 838 32 482.94 9.26 455 806 841 35 482.97 9.26 456 807 810 3 838.06 0.68 457 807 811 4 837.36 1.02 458 807 838 31 483.19 9.39 459 807 859 52 482.01 9.19 460 807 862 55 481.83 9.23 461 807 863 56 481.66 9.25 462 808 811 3 839.14 1.22 463 808 812 4 843.28 1.92 464 809 812 3 853.66 5.07 465 809 813 4 843.28 1.92 466 809 834 25 483.75 9.47 467 810 813 3 845.32 0.90 468 810 814 4 836.10 1.68 469 810 831 21 484.38 9.20 470 810 834 24 483.75 9.46 471 810 835 25 483.76 9.47 472 810 838 28 483.67 9.47 473 810 863 53 481.98 9.17 474 810 916 106 311.57 83.01 475 811 814 3 840.77 0.66 476 811 863 52 481.79 9.26 477 811 866 55 481.69 9.26 478 811 867 56 481.43 9.38 479 813 827 14 484.62 9.18 480 813 830 17 484.62 9.18 481 813 831 18 484.48 9.23 482 813 834 21 484.48 9.24 483 813 914 101 312.69 83.43 484 814 831 17 484.62 9.18 485 814 867 53 481.75 9.28 486 814 913 99 312.72 83.44 487 814 914 100 312.48 83.48 488 815 906 91 329.12 75.97 489 815 911 96 318.23 81.14 490 815 912 97 316.25 81.80 491 815 913 98 312.66 83.48 492 815 914 99 312.56 83.52 493 816 821 5 578.79 12.46 494 816 824 8 486.90 8.60 495 816 827 11 486.51 8.63 496 816 911 95 324.06 78.24 497 816 912 96 318.16 81.11 498 816 914 98 316.12 81.78 499 817 820 3 802.91 0.81 500 817 821 4 764.36 2.91 501 817 910 93 326.24 77.18 502 817 911 94 324.08 78.25 503 818 909 91 328.94 76.06 504 818 910 92 326.43 77.13 505 818 912 94 326.21 77.19 506 820 824 4 709.43 7.83 507 821 824 3 796.67 2.67 508 821 827 6 753.62 27.72 509 823 826 3 859.26 3.54 510 823 827 4 828.53 2.77 511 824 827 3 835.25 0.14 512 824 828 4 833.43 0.77 513 825 828 3 835.25 0.14 514 825 829 4 833.43 0.77 515 826 829 3 844.25 3.41 516 826 830 4 833.42 0.77 517 827 830 3 844.36 3.35 518 827 831 4 821.50 6.73 519 827 914 87 323.14 78.38 520 828 831 3 849.27 4.20 521 828 832 4 831.24 2.06 522 829 832 3 834.00 3.44 523 829 833 4 834.01 3.43 524 830 833 3 836.39 2.23 525 830 834 4 834.01 3.43 526 831 834 3 844.88 4.75 527 831 835 4 836.07 0.65 528 831 914 83 316.98 81.30 529 831 916 85 316.06 81.45 530 832 835 3 839.05 1.58 531 832 836 4 836.07 0.64 532 833 836 3 838.50 1.85 533 833 837 4 830.88 1.95 534 834 837 3 837.38 1.30 535 834 838 4 837.39 1.30 536 835 838 3 842.99 1.49 537 835 839 4 821.91 3.78 538 835 856 21 482.90 9.09 539 835 916 81 311.77 82.92 540 836 839 3 848.22 1.12 541 836 840 4 839.97 5.25 542 837 840 3 840.47 5.00 543 837 841 4 839.95 5.26 544 838 841 3 841.21 4.63 545 838 842 4 836.95 3.25 546 838 856 18 483.04 9.02 547 838 859 21 482.87 9.08 548 838 863 25 482.80 9.08 549 839 842 3 837.70 2.88 550 839 843 4 823.14 4.80 551 839 856 17 483.06 9.01 552 840 843 3 839.88 1.79 553 840 844 4 823.13 4.79 554 841 844 3 845.57 0.67 555 842 845 3 845.19 0.86 556 842 846 4 828.57 2.10 557 842 852 10 484.80 8.23 558 842 855 13 483.30 8.96 559 842 856 14 483.20 9.01 560 842 859 17 483.23 9.00 561 843 846 3 828.57 2.10 562 843 852 9 484.88 8.20 563 846 851 5 715.54 18.46 564 846 852 6 561.29 2.19 565 846 855 9 581.61 7.97 566 848 851 3 839.30 3.33 567 848 852 4 808.96 7.51 568 849 852 3 844.36 2.21 569 849 853 4 828.64 2.33 570 850 853 3 831.58 0.97 571 850 854 4 828.66 2.33 572 851 854 3 828.89 2.45 573 851 855 4 840.37 5.34 574 852 855 3 841.59 4.74 575 852 856 4 831.53 0.92 576 853 856 3 842.38 4.33 577 853 857 4 831.54 0.92 578 854 857 3 848.62 3.35 579 854 858 4 831.54 0.92 580 855 858 3 838.08 1.93 581 855 859 4 815.70 5.45 582 856 859 3 840.69 0.62 583 856 860 4 826.88 1.30 584 857 860 3 842.74 0.40 585 857 861 4 836.55 3.48 586 857 917 60 311.45 82.99 587 858 861 3 841.70 0.92 588 858 862 4 839.85 1.84 589 859 862 3 839.85 1.84 590 859 863 4 839.85 1.84 591 860 863 3 837.83 0.83 592 860 864 4 833.23 1.48 593 860 916 56 311.54 83.03 594 860 917 57 311.53 83.03 595 861 864 3 836.73 3.25 596 861 865 4 837.16 1.95 597 861 917 56 311.53 83.03 598 862 865 3 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