BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 671 675 4 831.62 1.51 2 672 675 3 840.37 2.80 3 672 676 4 832.09 1.28 4 673 676 3 840.01 0.52 5 673 677 4 835.92 0.58 6 673 723 50 111.39 41.51 7 673 806 133 110.66 41.86 8 673 834 161 110.64 41.87 9 673 837 164 110.64 41.87 10 674 677 3 837.05 1.15 11 674 678 4 838.13 2.72 12 674 802 128 111.03 41.70 13 674 840 166 112.24 42.30 14 674 841 167 111.99 42.18 15 675 678 3 844.73 0.57 16 675 679 4 836.07 3.76 17 676 679 3 841.23 1.19 18 676 680 4 836.07 3.76 19 676 723 47 114.82 41.56 20 677 680 3 859.03 0.24 21 677 681 4 841.22 1.19 22 677 722 45 116.22 41.45 23 677 805 128 112.58 42.45 24 677 806 129 112.58 42.45 25 677 809 132 114.57 41.45 26 678 681 3 858.91 0.30 27 678 805 127 114.92 41.55 28 680 718 38 124.53 44.32 29 680 721 41 124.55 44.34 30 680 722 42 124.44 44.36 31 681 718 37 124.89 44.48 32 681 804 123 124.33 44.31 33 681 805 124 124.17 44.40 34 681 808 127 124.35 44.33 35 682 714 32 128.53 42.91 36 682 718 36 124.98 44.43 37 684 766 82 269.14 89.91 38 685 707 22 269.11 89.90 39 685 710 25 268.81 89.93 40 685 711 26 247.68 84.33 41 685 714 29 254.84 86.53 42 685 765 80 268.84 89.92 43 685 766 81 269.19 89.89 44 686 707 21 269.15 89.90 45 686 710 24 269.14 89.89 46 686 765 79 269.13 89.90 47 686 766 80 269.20 89.89 48 686 767 81 269.20 89.89 49 687 703 16 269.19 89.89 50 687 706 19 269.18 89.89 51 687 707 20 269.16 89.90 52 687 710 23 269.17 89.89 53 687 765 78 269.20 89.89 54 687 767 80 269.48 90.00 55 687 768 81 269.21 89.89 56 688 710 22 269.25 89.89 57 688 767 79 269.56 89.97 58 689 699 10 269.56 89.97 59 689 700 11 269.55 89.97 60 689 703 14 269.54 89.97 61 689 767 78 269.56 89.97 62 689 768 79 269.56 89.97 63 689 774 85 269.73 89.99 64 690 693 3 855.59 1.34 65 691 767 76 269.73 89.93 66 691 774 83 269.79 89.96 67 695 700 5 793.35 2.83 68 695 773 78 309.12 91.05 69 695 774 79 308.69 90.98 70 695 777 82 309.51 90.95 71 696 699 3 840.99 4.67 72 696 700 4 824.34 4.34 73 697 700 3 842.74 3.79 74 697 701 4 823.57 4.79 75 697 777 80 315.66 93.36 76 697 778 81 315.00 93.03 77 697 779 82 315.00 93.03 78 697 880 183 177.81 14.18 79 697 887 190 132.72 9.37 80 697 889 192 132.01 9.71 81 698 701 3 858.57 0.35 82 698 702 4 823.60 4.81 83 698 887 189 132.08 9.68 84 698 888 190 132.03 9.71 85 698 889 191 131.94 9.71 86 699 702 3 865.43 3.52 87 699 703 4 830.70 1.25 88 700 703 3 857.59 7.44 89 700 704 4 830.84 1.18 90 700 770 70 332.16 90.33 91 700 774 74 332.19 90.33 92 700 777 77 332.20 90.33 93 700 778 78 332.18 90.33 94 701 704 3 850.39 6.75 95 701 705 4 830.83 1.18 96 701 778 77 332.22 90.33 97 701 780 79 332.22 90.33 98 701 876 175 181.18 14.84 99 701 880 179 165.44 6.96 100 701 889 188 131.90 9.91 101 702 705 3 846.11 4.60 102 702 706 4 838.00 4.76 103 702 889 187 131.88 9.92 104 702 890 188 132.17 10.14 105 703 706 3 838.00 4.76 106 703 707 4 835.57 3.55 107 703 768 65 333.83 90.53 108 704 707 3 838.00 4.77 109 704 708 4 823.40 2.52 110 704 764 60 333.69 90.47 111 704 768 64 335.88 89.84 112 704 770 66 336.54 90.19 113 704 780 76 334.77 90.31 114 705 708 3 844.12 5.53 115 705 709 4 843.44 5.87 116 705 889 184 138.23 13.09 117 705 890 185 138.03 13.19 118 705 891 186 136.02 13.18 119 705 892 187 137.88 14.55 120 705 895 190 133.52 12.50 121 706 709 3 843.26 3.71 122 706 710 4 840.20 1.22 123 706 892 186 139.22 15.23 124 707 710 3 854.03 5.70 125 707 711 4 840.26 1.25 126 707 764 57 338.31 89.75 127 707 765 58 338.38 89.76 128 707 768 61 339.75 89.10 129 708 711 3 860.26 4.79 130 708 712 4 842.23 0.26 131 708 745 37 338.62 88.22 132 708 895 187 140.48 16.03 133 709 712 3 868.82 0.52 134 709 713 4 842.24 0.26 135 709 892 183 140.71 16.08 136 709 893 184 140.75 16.09 137 709 894 185 140.66 16.09 138 709 895 186 140.47 16.04 139 710 713 3 874.44 2.29 140 710 714 4 842.25 0.26 141 711 714 3 838.56 1.58 142 711 715 4 838.53 1.57 143 711 745 34 344.97 86.11 144 711 752 41 345.39 86.28 145 712 715 3 844.40 4.51 146 712 716 4 838.57 1.59 147 712 737 25 345.19 86.09 148 712 741 29 345.05 86.05 149 712 745 33 345.30 86.20 150 712 894 182 140.91 16.23 151 712 895 183 140.80 16.20 152 712 898 186 134.77 19.25 153 713 716 3 856.39 1.48 154 713 717 4 842.80 0.52 155 713 894 181 143.62 17.58 156 714 717 3 859.87 5.11 157 714 718 4 835.78 4.02 158 715 718 3 856.94 6.56 159 715 719 4 842.82 0.50 160 715 744 29 346.61 85.56 161 715 745 30 346.69 85.56 162 716 719 3 844.15 0.12 163 716 720 4 847.12 2.65 164 716 737 21 348.23 84.73 165 717 720 3 849.72 1.36 166 717 721 4 847.13 2.65 167 718 721 3 853.41 3.21 168 718 722 4 847.13 2.66 169 718 744 26 353.97 82.01 170 718 808 90 353.96 81.98 171 718 809 91 353.92 81.98 172 718 812 94 353.77 81.88 173 719 722 3 864.88 2.53 174 719 725 6 574.69 10.85 175 719 737 18 353.93 81.94 176 719 812 93 353.73 81.84 177 720 725 5 689.05 46.32 178 720 726 6 557.09 2.04 179 722 725 3 839.32 2.52 180 722 809 87 354.05 81.95 181 722 812 90 354.10 81.92 182 722 834 112 243.87 26.79 183 723 830 107 244.46 27.07 184 723 834 111 243.92 26.80 185 724 727 3 837.94 1.21 186 724 830 106 244.46 27.07 187 725 728 3 855.52 2.18 188 725 733 8 540.23 10.85 189 725 736 11 529.75 15.58 190 725 737 12 529.73 15.56 191 725 812 87 354.20 81.89 192 725 830 105 244.50 27.05 193 726 733 7 614.03 18.62 194 728 732 4 835.00 4.17 195 728 733 5 797.11 1.35 196 728 813 85 354.50 81.79 197 728 826 98 244.78 26.94 198 728 827 99 244.78 26.94 199 728 830 102 244.65 27.00 200 729 732 3 839.27 6.27 201 729 733 4 833.20 5.08 202 729 822 93 264.29 21.26 203 729 823 94 260.23 23.27 204 730 733 3 835.01 4.17 205 730 734 4 835.00 4.17 206 731 734 3 859.85 0.46 207 731 735 4 835.01 4.17 208 731 822 91 274.89 15.96 209 732 735 3 840.73 1.93 210 732 736 4 834.51 4.40 211 732 821 89 274.89 15.96 212 732 822 90 274.89 15.96 213 732 823 91 275.45 15.68 214 733 736 3 858.90 5.19 215 733 737 4 838.99 2.79 216 734 737 3 846.51 6.54 217 734 738 4 831.62 3.23 218 735 738 3 837.80 4.58 219 735 739 4 832.20 3.51 220 735 821 86 278.22 14.31 221 736 739 3 854.61 3.81 222 736 740 4 823.10 1.03 223 736 812 76 382.12 68.17 224 736 821 85 284.47 11.19 225 736 827 91 272.22 13.21 226 737 740 3 860.60 0.33 227 737 741 4 830.01 2.44 228 737 812 75 384.56 66.95 229 738 741 3 875.86 3.48 230 738 898 160 182.36 43.08 231 738 902 164 173.42 47.29 232 739 815 76 390.26 63.81 233 739 817 78 342.08 37.48 234 739 902 163 173.47 47.31 235 739 905 166 173.32 47.37 236 739 906 167 173.01 47.43 237 739 911 172 155.63 55.37 238 740 743 3 851.39 4.68 239 740 744 4 832.28 1.30 240 740 811 71 392.42 63.03 241 740 812 72 392.37 63.05 242 740 815 75 343.22 38.49 243 741 744 3 833.59 0.66 244 741 745 4 827.09 2.12 245 741 898 157 194.41 49.08 246 741 899 158 194.41 49.08 247 742 745 3 856.49 5.45 248 742 746 4 838.45 3.57 249 742 870 128 248.90 15.16 250 742 873 131 245.31 17.00 251 742 874 132 244.95 17.18 252 742 877 135 247.74 23.57 253 743 746 3 840.61 0.07 254 743 747 4 827.14 2.08 255 743 811 68 398.98 60.00 256 743 866 123 260.49 9.25 257 743 870 127 248.88 15.20 258 744 747 3 842.88 0.39 259 744 748 4 828.96 2.99 260 744 808 64 399.05 59.99 261 744 811 67 399.02 59.98 262 744 812 68 399.02 59.97 263 745 748 3 842.79 1.24 264 745 749 4 824.97 1.10 265 746 749 3 857.12 1.71 266 746 750 4 828.71 3.00 267 746 787 41 426.38 64.98 268 746 790 44 426.41 64.98 269 746 869 123 258.32 18.94 270 746 873 127 253.73 21.30 271 747 750 3 845.05 6.46 272 747 751 4 833.44 0.65 273 747 790 43 426.38 64.97 274 747 794 47 426.28 64.92 275 747 804 57 407.90 55.76 276 747 807 60 407.75 55.72 277 747 808 61 407.63 55.70 278 747 811 64 407.59 55.69 279 747 862 115 274.06 11.07 280 747 863 116 273.80 11.15 281 747 866 119 269.02 13.53 282 748 751 3 842.48 7.75 283 748 752 4 830.64 2.05 284 748 804 56 407.88 55.78 285 748 808 60 407.63 55.70 286 749 752 3 833.20 3.10 287 749 753 4 830.18 2.46 288 749 762 13 429.14 65.28 289 749 763 14 429.12 65.28 290 749 764 15 428.45 64.96 291 749 787 38 426.69 64.86 292 750 753 3 852.79 3.86 293 750 754 4 830.19 2.45 294 750 791 41 426.68 64.84 295 750 794 44 426.56 64.78 296 751 754 3 851.73 4.40 297 751 755 4 830.19 2.45 298 751 794 43 426.56 64.78 299 751 799 48 408.33 55.66 300 751 800 49 408.36 55.67 301 751 801 50 408.28 55.63 302 751 804 53 408.24 55.62 303 752 755 3 850.31 4.71 304 752 756 4 811.57 3.45 305 752 763 11 429.20 65.25 306 752 764 12 429.24 65.23 307 753 756 3 844.39 0.72 308 753 757 4 806.25 0.80 309 753 758 5 758.44 11.22 310 753 759 6 552.84 3.57 311 753 761 8 536.15 11.86 312 753 762 9 429.50 65.11 313 753 763 10 429.20 65.25 314 753 791 38 426.72 64.84 315 754 758 4 615.56 2.14 316 754 759 5 552.83 3.57 317 754 791 37 426.75 64.84 318 754 794 40 426.59 64.78 319 754 795 41 426.57 64.77 320 756 763 7 536.21 11.80 321 757 760 3 848.51 3.46 322 757 761 4 726.60 1.97 323 757 763 6 536.22 11.81 324 759 788 29 427.11 64.78 325 759 791 32 426.98 64.75 326 759 792 33 426.86 64.78 327 761 769 8 429.09 64.97 328 761 770 9 428.88 64.92 329 761 771 10 428.78 64.96 330 762 769 7 429.08 64.95 331 762 770 8 428.88 64.92 332 762 775 13 428.87 64.92 333 762 784 22 427.10 64.78 334 762 787 25 427.09 64.78 335 762 788 26 427.08 64.78 336 763 766 3 899.70 2.49 337 763 769 6 527.48 15.72 338 764 768 4 791.89 5.84 339 764 770 6 599.50 7.54 340 764 787 23 427.07 64.78 341 765 768 3 826.09 0.38 342 765 769 4 809.93 3.17 343 766 769 3 890.34 6.00 344 769 774 5 822.44 2.54 345 770 774 4 822.40 2.54 346 770 775 5 813.27 2.02 347 770 778 8 767.84 6.27 348 771 774 3 839.85 2.17 349 771 775 4 827.25 0.54 350 772 775 3 847.50 0.81 351 772 776 4 836.04 4.92 352 772 781 9 549.29 7.44 353 772 788 16 428.72 64.01 354 773 776 3 845.04 0.42 355 773 777 4 829.73 0.68 356 774 777 3 827.22 3.87 357 774 778 4 817.69 4.27 358 775 778 3 844.27 0.75 359 775 780 5 701.38 0.11 360 775 781 6 622.01 28.94 361 775 784 9 534.45 11.50 362 775 788 13 534.44 11.50 363 776 779 3 844.27 0.75 364 776 781 5 622.27 28.82 365 778 876 98 314.05 50.68 366 779 876 97 314.09 50.69 367 779 887 108 274.49 71.52 368 780 783 3 854.75 0.07 369 780 784 4 819.84 5.19 370 780 787 7 819.85 5.17 371 780 873 93 316.89 49.30 372 780 876 96 314.07 50.70 373 781 784 3 847.90 7.99 374 781 785 4 819.85 5.18 375 782 785 3 841.65 1.21 376 782 786 4 837.14 3.46 377 783 786 3 854.02 4.92 378 783 787 4 827.95 1.12 379 783 872 89 317.28 49.45 380 783 873 90 317.28 49.49 381 783 876 93 315.14 51.05 382 784 787 3 832.07 0.94 383 784 788 4 827.89 1.10 384 785 788 3 842.85 5.98 385 785 789 4 830.93 2.62 386 786 789 3 837.37 1.72 387 786 790 4 828.53 3.85 388 786 868 82 332.15 42.05 389 786 869 83 318.36 48.95 390 786 872 86 318.86 49.40 391 787 790 3 840.79 2.28 392 787 791 4 834.11 1.06 393 787 869 82 318.35 48.95 394 787 873 86 318.12 49.91 395 788 791 3 845.88 0.47 396 788 792 4 845.89 0.47 397 789 792 3 845.89 0.47 398 789 793 4 845.89 0.47 399 789 796 7 805.67 1.62 400 790 793 3 852.12 2.65 401 790 794 4 845.89 0.47 402 790 862 72 334.62 41.36 403 790 866 76 332.99 42.47 404 790 869 79 319.29 49.37 405 791 794 3 859.46 2.92 406 792 795 3 847.18 3.23 407 792 796 4 830.52 0.76 408 793 796 3 843.26 1.27 409 793 797 4 817.16 7.44 410 793 862 69 335.10 41.43 411 794 798 4 800.16 1.02 412 794 799 5 633.35 8.91 413 794 804 10 552.50 5.38 414 794 862 68 335.14 41.44 415 795 798 3 836.41 8.07 416 797 855 58 367.81 56.80 417 797 858 61 365.76 56.05 418 797 862 65 363.89 55.16 419 798 855 57 367.95 56.87 420 799 803 4 829.81 0.56 421 799 804 5 801.30 3.37 422 799 807 8 785.36 9.15 423 799 855 56 367.95 56.87 424 799 859 60 367.51 56.95 425 799 862 63 367.43 56.95 426 800 803 3 842.23 3.31 427 800 804 4 829.83 0.56 428 801 804 3 851.08 1.12 429 801 805 4 829.84 0.55 430 802 805 3 852.15 0.59 431 802 806 4 844.91 0.91 432 802 841 39 391.93 49.02 433 803 806 3 850.91 2.07 434 803 807 4 844.91 0.91 435 803 841 38 391.92 49.02 436 803 855 52 372.03 58.88 437 803 859 56 371.50 58.95 438 804 807 3 850.90 2.08 439 804 808 4 838.07 2.52 440 805 808 3 847.69 2.29 441 805 809 4 844.87 0.88 442 806 809 3 843.65 1.49 443 806 810 4 831.70 0.70 444 806 834 28 394.11 48.35 445 806 837 31 392.73 48.91 446 806 838 32 392.70 48.91 447 807 810 3 843.56 3.50 448 807 811 4 831.73 0.70 449 807 838 31 392.73 48.91 450 807 859 52 371.69 59.05 451 807 862 55 370.75 59.40 452 807 863 56 370.33 59.40 453 808 811 3 848.88 6.16 454 808 812 4 839.60 4.63 455 809 812 3 870.07 0.38 456 809 813 4 856.43 2.73 457 809 834 25 394.21 48.40 458 810 813 3 859.52 1.19 459 810 814 4 835.87 2.77 460 810 831 21 394.69 48.21 461 810 834 24 394.18 48.42 462 810 835 25 394.19 48.44 463 810 838 28 392.91 49.00 464 810 863 53 370.69 59.57 465 810 916 106 245.90 107.89 466 811 814 3 863.49 0.80 467 811 863 52 370.52 59.51 468 811 866 55 370.07 59.72 469 811 867 56 369.92 59.79 470 813 827 14 394.99 48.18 471 813 830 17 394.93 48.16 472 813 831 18 394.79 48.20 473 813 834 21 394.31 48.41 474 813 914 101 248.84 108.48 475 814 831 17 394.91 48.16 476 814 867 53 370.11 59.71 477 814 913 99 249.85 108.03 478 814 914 100 248.28 108.67 479 815 906 91 273.29 97.57 480 815 911 96 255.94 105.78 481 815 912 97 254.56 106.19 482 815 913 98 249.52 108.20 483 816 821 5 649.72 3.54 484 816 824 8 581.11 30.76 485 816 827 11 398.88 46.40 486 816 911 95 256.20 105.64 487 816 912 96 255.09 106.09 488 816 914 98 254.11 106.41 489 817 820 3 824.91 0.25 490 817 821 4 809.63 6.91 491 817 910 93 259.48 104.32 492 817 911 94 258.17 104.67 493 818 909 91 267.62 100.44 494 818 910 92 259.60 104.28 495 818 912 94 258.64 104.74 496 820 824 4 744.01 6.30 497 821 824 3 815.56 8.96 498 821 827 6 762.53 11.57 499 823 826 3 870.01 0.33 500 823 827 4 823.81 3.54 501 824 827 3 849.83 1.37 502 824 828 4 823.71 3.49 503 825 828 3 851.65 0.46 504 825 829 4 835.37 2.18 505 826 829 3 846.49 3.38 506 826 830 4 834.96 2.39 507 827 830 3 839.67 0.04 508 827 831 4 829.18 0.50 509 827 914 87 249.22 108.36 510 828 831 3 850.53 4.39 511 828 832 4 830.13 0.02 512 829 832 3 842.63 2.21 513 829 833 4 833.81 1.06 514 830 833 3 854.35 2.25 515 830 834 4 836.56 0.32 516 831 834 3 843.54 1.75 517 831 835 4 831.80 0.01 518 831 914 83 248.39 108.73 519 831 916 85 247.82 108.85 520 832 835 3 855.38 1.74 521 832 836 4 830.99 0.41 522 833 836 3 855.38 1.74 523 833 837 4 832.04 0.12 524 834 837 3 852.25 0.18 525 834 838 4 832.04 0.12 526 835 838 3 843.45 4.36 527 835 839 4 821.10 1.83 528 835 856 21 372.75 59.40 529 835 916 81 245.38 108.14 530 836 839 3 842.12 4.92 531 836 840 4 832.34 7.46 532 837 840 3 839.69 6.12 533 837 841 4 833.43 6.91 534 838 841 3 846.32 2.92 535 838 842 4 820.27 0.33 536 838 856 18 372.77 59.39 537 838 859 21 372.48 59.26 538 838 863 25 372.08 59.30 539 839 842 3 848.68 0.69 540 839 843 4 820.15 0.29 541 839 856 17 372.86 59.34 542 840 843 3 838.74 5.66 543 840 844 4 808.58 5.50 544 842 846 4 821.85 3.73 545 842 852 10 373.35 59.51 546 842 855 13 373.25 59.49 547 842 856 14 373.26 59.50 548 842 859 17 373.15 59.45 549 843 846 3 851.72 1.15 550 843 852 9 373.35 59.51 551 846 851 5 799.13 4.15 552 846 852 6 666.90 8.52 553 846 855 9 662.93 15.08 554 848 851 3 862.44 6.15 555 848 852 4 817.54 1.50 556 849 852 3 850.37 12.19 557 849 853 4 815.51 2.51 558 850 853 3 859.61 7.57 559 850 854 4 819.31 3.06 560 851 854 3 834.03 4.29 561 851 855 4 828.55 1.55 562 852 855 3 837.53 3.14 563 852 856 4 830.40 2.48 564 853 856 3 833.78 1.27 565 853 857 4 830.88 2.71 566 854 857 3 851.31 7.50 567 854 858 4 830.82 2.75 568 855 858 3 859.80 3.27 569 855 859 4 830.82 2.75 570 856 859 3 855.53 5.40 571 856 860 4 826.64 0.65 572 857 860 3 854.67 3.80 573 857 861 4 833.00 3.42 574 857 917 60 245.21 108.06 575 858 861 3 844.72 2.44 576 858 862 4 833.00 3.42 577 859 862 3 820.24 2.35 578 859 863 4 819.69 2.06 579 860 863 3 853.84 7.04 580 860 864 4 819.89 1.96 581 860 916 56 245.16 108.09 582 860 917 57 245.21 108.06 583 861 864 3 855.64 9.37 584 861 865 4 843.38 3.27 585 861 917 56 245.28 108.09 586 862 865 3 862.03 1.87 587 862 866 4 848.64 0.70 588 863 866 3 862.03 5.99 589 863 916 53 245.91 107.88 590 864 867 3 871.28 1.37 591 864 868 4 863.63 5.19 592 864 915 51 245.91 107.88 593 864 916 52 246.02 107.83 594 864 917 53 245.88 107.89 595 865 868 3 872.39 1.93 596 865 869 4 850.17 0.06 597 866 869 3 850.28 0.01 598 866 870 4 848.71 0.79 599 867 870 3 848.71 0.79 600 867 871 4 848.70 0.79 601 867 913 46 253.13 106.70 602 867 915 48 251.36 107.25 603 868 871 3 846.92 8.06 604 868 872 4 835.99 2.60 605 868 915 47 252.99 106.61 606 869 872 3 833.70 1.46 607 869 873 4 825.10 2.84 608 870 873 3 844.38 1.61 609 870 874 4 820.56 0.65 610 871 874 3 841.33 3.15 611 871 875 4 829.20 5.98 612 871 906 35 274.12 97.76 613 871 907 36 273.91 97.87 614 872 875 3 846.83 2.83 615 872 876 4 820.99 0.43 616 873 876 3 840.32 5.60 617 873 877 4 820.96 0.42 618 874 877 3 845.84 4.87 619 874 878 4 830.49 5.75 620 874 903 29 274.39 97.73 621 874 906 32 274.19 97.79 622 874 907 33 274.13 97.81 623 875 878 3 845.64 1.80 624 875 879 4 830.47 5.76 625 875 907 32 274.17 97.78 626 876 879 3 854.27 1.83 627 876 880 4 830.47 5.77 628 877 880 3 839.13 1.44 629 877 881 4 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