BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 178.24 0.69 2 4 204 200 171.60 0.53 3 4 205 201 167.44 0.41 4 4 224 220 119.61 0.24 5 4 227 223 119.14 0.12 6 5 35 30 611.83 31.04 7 5 36 31 611.77 31.05 8 5 37 32 611.76 31.05 9 5 38 33 611.73 31.05 10 5 205 200 170.71 0.09 11 6 36 30 611.82 31.03 12 6 37 31 611.77 31.05 13 6 38 32 611.76 31.05 14 6 221 215 119.55 0.23 15 6 227 221 119.55 0.23 16 6 228 222 119.53 0.24 17 6 229 223 119.53 0.24 18 6 246 240 33.34 0.26 19 6 247 241 25.29 0.33 20 7 38 31 611.77 31.05 21 7 40 33 611.76 31.05 22 7 90 83 196.44 0.32 23 7 124 117 182.85 0.26 24 7 161 154 182.80 0.24 25 7 205 198 182.20 0.35 26 7 207 200 170.02 0.42 27 7 227 220 119.63 0.24 28 7 228 221 119.53 0.23 29 7 229 222 119.53 0.24 30 8 37 29 611.86 31.03 31 8 38 30 611.82 31.03 32 8 39 31 611.77 31.05 33 8 40 32 611.76 31.05 34 8 229 221 119.55 0.23 35 8 231 223 119.52 0.23 36 8 240 232 50.73 0.06 37 8 243 235 41.23 0.72 38 8 244 236 35.37 0.67 39 9 40 31 611.76 31.05 40 9 229 220 119.67 0.22 41 9 230 221 119.53 0.24 42 9 231 222 119.52 0.24 43 10 40 30 611.82 31.03 44 10 41 31 611.77 31.05 45 10 42 32 611.76 31.05 46 10 231 221 119.52 0.23 47 10 235 225 78.36 0.88 48 10 240 230 51.27 0.18 49 11 42 31 611.77 31.05 50 11 64 53 285.94 0.91 51 11 92 81 197.02 0.01 52 11 95 84 194.70 0.57 53 11 230 219 120.70 0.53 54 11 231 220 119.63 0.23 55 11 232 221 119.50 0.24 56 11 235 224 87.34 0.67 57 12 15 3 863.93 1.03 58 12 21 9 613.01 30.87 59 12 24 12 612.88 30.85 60 12 25 13 612.89 30.85 61 12 41 29 611.87 31.03 62 12 42 30 611.79 31.04 63 12 43 31 611.78 31.05 64 12 44 32 611.77 31.05 65 12 51 39 526.73 24.23 66 12 64 52 286.17 1.01 67 12 235 223 116.40 0.73 68 12 236 224 83.98 0.66 69 13 44 31 611.77 31.05 70 13 64 51 286.16 1.01 71 13 95 82 197.03 0.02 72 13 97 84 196.91 0.03 73 13 170 157 195.81 0.10 74 13 232 219 120.69 0.53 75 15 21 6 821.16 1.23 76 15 44 29 611.86 31.04 77 15 236 221 119.17 0.14 78 16 20 4 826.31 0.46 79 16 21 5 821.14 1.22 80 16 47 31 611.75 31.05 81 17 20 3 864.14 1.14 82 17 21 4 836.11 3.69 83 17 47 30 611.75 31.05 84 18 21 3 864.14 1.13 85 18 22 4 836.12 3.69 86 18 47 29 611.70 31.04 87 18 50 32 581.25 22.18 88 19 22 3 854.13 3.88 89 19 23 4 841.87 1.86 90 20 23 3 856.03 2.93 91 20 24 4 855.76 3.06 92 21 24 3 847.38 1.40 93 21 25 4 840.59 4.53 94 21 44 23 611.87 31.04 95 21 47 26 610.89 31.35 96 21 50 29 561.41 23.20 97 22 25 3 859.78 5.07 98 22 26 4 840.59 4.53 99 22 50 28 561.41 23.20 100 23 26 3 842.88 3.39 101 23 27 4 840.08 4.79 102 24 27 3 848.21 0.72 103 24 28 4 837.36 3.44 104 24 235 211 112.74 3.25 105 24 236 212 82.84 1.22 106 24 237 213 81.59 0.61 107 25 28 3 851.49 0.92 108 25 29 4 836.51 3.86 109 25 50 25 543.50 32.13 110 25 51 26 527.10 24.04 111 25 54 29 289.42 1.23 112 25 55 30 288.07 0.78 113 26 29 3 852.76 0.28 114 26 30 4 841.04 1.59 115 27 30 3 849.25 0.21 116 27 31 4 852.76 4.27 117 28 31 3 855.28 3.01 118 28 33 5 827.92 0.67 119 28 41 13 611.95 30.98 120 28 55 27 288.07 0.78 121 28 59 31 286.62 1.13 122 28 240 212 50.98 0.01 123 28 241 213 50.80 0.01 124 28 244 216 50.61 0.10 125 29 54 25 288.35 0.70 126 29 55 26 288.06 0.79 127 29 56 27 288.01 0.81 128 31 241 210 50.80 0.01 129 32 245 213 43.87 0.39 130 33 58 25 286.60 1.11 131 33 59 26 286.56 1.10 132 33 241 208 50.72 0.04 133 33 244 211 43.34 0.21 134 33 245 212 41.22 0.61 135 34 37 3 846.86 0.53 136 34 245 211 38.49 0.43 137 34 248 214 30.34 0.49 138 37 244 207 38.39 0.39 139 38 90 52 196.43 0.32 140 38 205 167 182.79 0.24 141 39 59 20 286.25 1.04 142 39 60 21 286.24 1.04 143 39 240 201 50.79 0.03 144 39 244 205 35.37 0.67 145 40 60 20 286.24 1.04 146 40 61 21 286.22 1.03 147 40 62 22 286.20 1.02 148 40 90 50 196.50 0.33 149 40 124 84 192.17 1.46 150 41 55 14 287.83 0.72 151 41 59 18 286.50 1.08 152 41 60 19 286.29 1.04 153 41 61 20 286.23 1.04 154 41 62 21 286.22 1.03 155 42 62 20 286.22 1.03 156 42 64 22 286.07 1.04 157 42 92 50 197.03 0.03 158 43 48 5 529.43 28.02 159 43 51 8 513.72 27.45 160 43 62 19 286.31 1.02 161 43 64 21 286.19 1.02 162 44 47 3 857.03 5.54 163 44 48 4 820.41 0.78 164 44 64 20 286.25 1.04 165 45 48 3 856.74 5.68 166 45 78 33 209.27 0.97 167 47 50 3 848.71 0.83 168 47 51 4 833.50 1.86 169 48 51 3 850.61 7.04 170 48 52 4 834.43 2.32 171 48 61 13 286.64 1.16 172 48 62 14 286.63 1.15 173 48 63 15 286.57 1.14 174 48 78 30 209.27 0.96 175 48 83 35 197.59 0.12 176 48 86 38 199.02 3.35 177 49 52 3 859.71 8.39 178 49 53 4 853.94 5.38 179 49 86 37 190.52 0.67 180 50 53 3 850.65 3.54 181 50 54 4 845.52 1.17 182 51 54 3 858.57 0.42 183 51 55 4 845.51 1.17 184 52 55 3 852.60 1.36 185 52 56 4 865.60 4.35 186 52 57 5 838.62 4.57 187 52 59 7 633.39 29.23 188 52 60 8 287.25 0.94 189 52 61 9 286.70 1.14 190 52 86 34 196.79 0.58 191 52 87 35 192.83 0.26 192 55 59 4 817.62 7.02 193 55 61 6 288.82 0.53 194 56 59 3 851.80 1.24 195 61 88 27 197.58 0.15 196 61 89 28 197.58 0.15 197 61 90 29 196.51 0.33 198 62 90 28 196.51 0.33 199 62 91 29 197.09 0.04 200 62 92 30 197.10 0.04 201 63 78 15 209.23 0.98 202 63 83 20 197.96 0.07 203 63 90 27 197.65 0.15 204 63 91 28 197.58 0.12 205 63 92 29 197.58 0.12 206 64 92 28 197.59 0.12 207 65 78 13 209.33 0.97 208 65 83 18 208.86 0.99 209 65 91 26 197.87 0.09 210 65 92 27 197.87 0.09 211 65 93 28 197.81 0.06 212 65 98 33 197.85 0.09 213 65 104 39 202.34 1.50 214 65 112 47 207.44 0.66 215 66 78 12 209.33 0.97 216 66 79 13 209.27 0.99 217 66 80 14 209.07 0.90 218 66 83 17 209.15 1.04 219 66 91 25 198.00 0.04 220 66 93 27 197.97 0.04 221 66 112 46 208.42 1.02 222 67 74 7 211.01 0.72 223 67 77 10 209.60 0.99 224 67 78 11 209.38 0.99 225 67 80 13 209.33 0.97 226 67 108 41 208.69 0.90 227 67 112 45 208.69 0.91 228 68 108 40 208.72 0.92 229 68 111 43 208.77 0.90 230 68 112 44 208.71 0.91 231 68 115 47 209.19 0.97 232 69 74 5 826.90 1.16 233 69 79 10 209.65 0.98 234 69 115 46 209.33 1.03 235 70 79 9 209.65 0.98 236 70 115 45 209.57 1.02 237 74 77 3 861.45 0.38 238 78 82 4 855.20 2.69 239 78 83 5 836.43 6.70 240 79 82 3 859.23 1.46 241 79 83 4 840.08 4.88 242 80 83 3 855.21 2.69 243 80 84 4 855.22 2.69 244 80 115 35 501.13 26.80 245 80 116 36 357.57 28.09 246 80 119 39 503.17 26.30 247 80 121 41 502.91 26.19 248 81 84 3 856.99 1.80 249 81 85 4 836.46 6.70 250 81 119 38 504.63 27.00 251 82 85 3 842.95 3.46 252 82 86 4 837.52 6.17 253 83 86 3 854.36 2.25 254 83 87 4 835.62 6.29 255 83 88 5 756.99 32.14 256 83 90 7 508.49 27.96 257 83 91 8 504.38 26.62 258 83 121 38 518.49 23.33 259 84 87 3 837.26 6.03 260 84 88 4 826.48 1.77 261 84 119 35 552.96 27.86 262 84 121 37 523.28 20.98 263 88 121 33 606.60 29.55 264 89 121 32 606.60 29.55 265 89 122 33 606.57 29.55 266 90 121 31 606.61 29.55 267 90 122 32 606.58 29.55 268 90 123 33 606.58 29.55 269 90 124 34 606.56 29.55 270 90 159 69 606.60 29.55 271 90 161 71 606.56 29.55 272 91 116 25 606.70 29.57 273 91 121 30 606.70 29.57 274 91 122 31 606.64 29.55 275 91 123 32 606.58 29.55 276 91 124 33 606.58 29.55 277 92 95 3 915.64 2.00 278 92 124 32 606.59 29.55 279 92 126 34 606.56 29.55 280 92 230 138 120.73 0.55 281 93 112 19 606.80 29.54 282 93 113 20 606.70 29.57 283 93 116 23 606.89 29.57 284 93 123 30 606.69 29.57 285 93 124 31 606.68 29.57 286 93 125 32 606.58 29.55 287 93 126 33 606.57 29.55 288 94 99 5 821.21 1.98 289 94 109 15 606.69 29.57 290 94 126 32 606.58 29.55 291 95 98 3 844.76 0.83 292 95 99 4 835.45 2.71 293 95 126 31 606.60 29.57 294 95 165 70 606.54 29.55 295 96 99 3 837.86 1.51 296 96 100 4 827.60 1.21 297 96 167 71 585.16 24.22 298 96 170 74 488.69 5.92 299 97 100 3 866.34 4.30 300 97 101 4 760.12 32.50 301 97 102 5 753.45 35.55 302 97 170 73 536.72 25.30 303 98 102 4 758.46 27.55 304 98 103 5 685.32 9.03 305 98 104 6 607.89 29.38 306 99 103 4 607.99 29.41 307 99 104 5 607.90 29.38 308 99 106 7 607.98 29.41 309 99 109 10 607.90 29.39 310 99 126 27 606.80 29.59 311 99 146 47 606.82 29.59 312 100 130 30 606.68 29.60 313 100 167 67 604.75 29.88 314 104 108 4 831.76 5.25 315 104 109 5 813.54 3.87 316 104 112 8 806.55 4.51 317 105 108 3 848.78 2.81 318 105 109 4 832.05 5.37 319 106 109 3 845.59 7.50 320 106 110 4 832.06 5.37 321 106 146 40 606.85 29.59 322 106 149 43 607.03 29.53 323 107 110 3 859.03 3.79 324 107 111 4 838.68 7.86 325 108 111 3 860.61 6.54 326 108 112 4 832.89 4.96 327 109 112 3 859.63 7.02 328 109 113 4 832.88 4.96 329 109 150 41 607.10 29.55 330 110 113 3 854.07 1.52 331 110 114 4 848.62 1.21 332 110 149 39 607.16 29.53 333 110 153 43 607.24 29.56 334 111 114 3 873.70 0.01 335 111 115 4 848.62 1.21 336 112 115 3 863.15 5.28 337 112 116 4 848.62 1.20 338 113 116 3 846.41 0.07 339 113 117 4 843.90 1.16 340 113 123 10 607.32 29.48 341 113 150 37 607.33 29.48 342 113 154 41 607.62 29.46 343 114 117 3 854.60 1.83 344 114 118 4 843.93 1.17 345 114 153 39 607.47 29.48 346 115 118 3 854.52 3.87 347 115 119 4 843.50 1.39 348 116 119 3 857.68 2.29 349 116 120 4 834.24 6.01 350 116 121 5 751.34 22.88 351 116 123 7 628.38 29.30 352 117 120 3 834.32 3.46 353 117 121 4 814.00 3.89 354 117 123 6 684.86 56.10 355 117 154 37 703.31 1.16 356 117 156 39 703.34 1.17 357 117 159 42 703.33 1.17 358 117 160 43 703.29 1.17 359 120 159 39 787.16 0.24 360 122 159 37 787.16 0.24 361 123 159 36 787.32 0.24 362 123 160 37 787.23 0.23 363 123 161 38 787.23 0.22 364 124 161 37 787.23 0.22 365 124 163 39 787.21 0.22 366 124 207 83 379.60 0.46 367 124 230 106 120.55 0.57 368 125 150 25 787.48 0.23 369 125 161 36 787.33 0.24 370 125 162 37 787.28 0.24 371 125 163 38 787.23 0.21 372 126 163 37 787.28 0.24 373 126 230 104 121.33 0.26 374 127 143 16 787.82 0.23 375 127 146 19 787.74 0.18 376 127 147 20 787.68 0.18 377 127 150 23 787.69 0.19 378 127 162 35 787.37 0.24 379 127 163 36 787.33 0.23 380 127 164 37 787.30 0.24 381 127 165 38 787.28 0.23 382 127 188 61 393.31 1.58 383 128 142 14 787.95 0.18 384 128 143 15 787.94 0.17 385 128 146 18 787.89 0.18 386 128 164 36 787.30 0.24 387 128 165 37 787.29 0.24 388 128 167 39 786.91 0.36 389 129 133 4 834.07 1.95 390 129 134 5 827.36 1.41 391 129 139 10 788.04 0.17 392 129 142 13 788.01 0.17 393 129 164 35 787.38 0.24 394 129 166 37 787.28 0.24 395 129 167 38 787.27 0.24 396 129 168 39 787.22 0.21 397 130 133 3 851.81 3.78 398 130 134 4 842.48 0.91 399 130 167 37 787.28 0.24 400 130 168 38 787.24 0.23 401 131 134 3 856.08 1.67 402 131 135 4 851.86 3.78 403 131 168 37 787.28 0.24 404 132 135 3 854.81 2.31 405 132 136 4 851.86 3.78 406 133 136 3 856.09 1.67 407 133 137 4 851.86 3.78 408 133 138 5 788.38 0.12 409 133 142 9 788.09 0.18 410 134 137 3 842.48 0.90 411 134 139 5 788.11 0.19 412 134 164 30 787.44 0.23 413 134 168 34 787.30 0.24 414 134 180 46 395.18 1.74 415 134 184 50 394.39 1.44 416 138 141 3 842.89 1.78 417 138 142 4 833.03 4.16 418 139 142 3 847.83 3.24 419 139 143 4 832.93 4.11 420 139 184 45 394.03 1.44 421 140 143 3 853.13 2.87 422 140 144 4 832.93 4.11 423 140 187 47 393.99 1.44 424 141 144 3 853.00 2.80 425 141 145 4 842.02 0.41 426 142 145 3 846.11 1.27 427 142 146 4 842.02 0.41 428 143 146 3 845.95 1.34 429 143 147 4 842.02 0.41 430 143 164 21 787.34 0.22 431 143 184 41 393.97 1.44 432 143 188 45 393.54 1.50 433 144 147 3 842.24 0.52 434 144 148 4 841.88 0.48 435 144 187 43 393.74 1.54 436 144 188 44 393.59 1.48 437 144 191 47 393.31 1.57 438 145 148 3 855.87 1.22 439 145 149 4 843.81 0.49 440 146 149 3 855.87 1.22 441 146 150 4 849.59 3.40 442 147 150 3 846.21 1.58 443 147 151 4 841.77 0.45 444 147 162 15 787.38 0.24 445 147 188 41 393.32 1.58 446 147 191 44 393.08 1.64 447 148 151 3 842.80 0.11 448 148 152 4 840.50 1.03 449 148 191 43 393.07 1.64 450 149 153 4 841.61 0.48 451 150 153 3 856.23 1.46 452 150 154 4 838.41 1.11 453 151 154 3 838.41 1.12 454 151 157 6 799.33 2.81 455 151 191 40 392.99 1.60 456 154 157 3 888.99 1.80 457 154 160 6 789.63 0.82 458 156 160 4 859.29 0.14 459 157 160 3 850.47 4.55 460 157 192 35 387.74 2.73 461 157 196 39 381.10 0.58 462 157 200 43 380.12 0.45 463 157 204 47 379.98 0.47 464 160 204 44 379.99 0.48 465 161 205 44 379.92 0.48 466 161 207 46 379.61 0.47 467 162 192 30 390.84 1.25 468 162 205 43 380.04 0.46 469 162 206 44 379.68 0.47 470 162 207 45 379.63 0.47 471 163 207 44 379.69 0.47 472 163 230 67 121.29 0.24 473 164 184 20 393.53 1.47 474 164 185 21 393.31 1.58 475 164 188 24 393.26 1.56 476 164 207 43 379.97 0.48 477 164 208 44 379.63 0.47 478 164 209 45 379.60 0.46 479 165 170 5 822.15 5.17 480 165 209 44 379.60 0.46 481 165 210 45 379.52 0.49 482 165 230 65 121.66 0.40 483 166 169 3 856.69 0.61 484 166 170 4 838.34 0.93 485 166 176 10 394.98 1.64 486 166 208 42 380.00 0.46 487 166 209 43 379.83 0.47 488 167 170 3 856.69 0.61 489 168 172 4 831.35 6.52 490 168 174 6 402.93 2.01 491 168 175 7 402.92 2.01 492 168 176 8 395.59 1.66 493 168 180 12 395.33 1.65 494 169 172 3 854.14 3.35 495 169 174 5 402.88 2.01 496 169 175 6 395.52 1.67 497 169 208 39 380.37 0.45 498 169 210 41 379.62 0.42 499 169 211 42 379.63 0.43 500 170 210 40 379.62 0.43 501 172 211 39 379.74 0.48 502 173 211 38 379.74 0.47 503 174 211 37 379.78 0.46 504 175 208 33 380.56 0.50 505 175 210 35 379.82 0.48 506 175 211 36 379.79 0.46 507 175 216 41 346.58 0.93 508 175 220 45 290.67 14.31 509 176 180 4 824.14 3.63 510 176 181 5 811.99 0.01 511 176 208 32 380.56 0.50 512 176 220 44 289.37 14.91 513 177 180 3 824.15 3.63 514 177 181 4 824.16 3.62 515 178 181 3 851.70 3.64 516 178 182 4 835.62 4.40 517 178 219 41 288.73 14.71 518 178 220 42 288.67 14.69 519 178 223 45 243.61 0.01 520 179 182 3 846.57 1.07 521 179 183 4 835.62 4.40 522 180 183 3 851.11 0.80 523 180 184 4 841.63 1.40 524 181 184 3 860.09 0.01 525 181 185 4 839.28 2.55 526 181 208 27 380.56 0.52 527 181 220 39 288.66 14.69 528 181 226 45 122.57 0.42 529 182 185 3 846.33 4.29 530 182 186 4 840.06 2.16 531 182 223 41 122.64 0.43 532 182 224 42 122.63 0.44 533 182 225 43 122.59 0.43 534 183 186 3 856.97 1.02 535 183 187 4 834.26 0.77 536 184 187 3 851.83 1.54 537 184 188 4 828.97 3.42 538 185 188 3 846.75 3.16 539 185 189 4 838.12 1.15 540 185 206 21 380.74 0.58 541 185 207 22 380.74 0.58 542 185 208 23 380.69 0.58 543 185 225 40 122.50 0.40 544 185 226 41 122.49 0.40 545 186 189 3 847.51 6.95 546 186 190 4 834.21 0.81 547 186 225 39 122.50 0.40 548 187 190 3 837.12 2.27 549 187 191 4 828.96 3.43 550 188 191 3 850.14 0.83 551 188 192 4 831.55 4.74 552 188 206 18 380.72 0.59 553 189 192 3 854.31 2.91 554 189 193 4 831.53 4.75 555 189 201 12 381.07 0.60 556 189 204 15 380.99 0.62 557 189 206 17 380.74 0.58 558 189 225 36 122.48 0.40 559 189 227 38 122.47 0.41 560 190 193 3 837.32 1.86 561 190 194 4 827.99 6.53 562 191 194 3 845.68 2.32 563 191 195 4 816.46 0.76 564 191 196 5 816.33 0.70 565 192 195 3 843.26 2.79 566 192 196 4 816.22 0.68 567 192 197 5 807.13 5.22 568 192 200 8 390.11 3.79 569 192 201 9 381.04 0.63 570 192 204 12 380.86 0.62 571 192 206 14 380.78 0.60 572 193 197 4 735.48 31.88 573 193 198 5 444.47 1.80 574 193 201 8 389.95 5.05 575 196 200 4 829.38 6.06 576 197 200 3 849.45 5.24 577 197 201 4 825.22 3.98 578 198 201 3 837.45 10.10 579 198 202 4 825.22 3.98 580 199 202 3 859.18 0.77 581 199 203 4 824.06 4.55 582 200 203 3 838.42 4.90 583 200 204 4 759.18 36.99 584 205 227 22 122.00 0.40 585 206 226 20 122.37 0.41 586 206 227 21 122.31 0.43 587 207 227 20 122.33 0.43 588 207 228 21 121.78 0.46 589 207 229 22 121.62 0.38 590 207 230 23 121.42 0.29 591 208 212 4 863.21 2.73 592 208 220 12 231.32 5.42 593 208 226 18 122.48 0.41 594 208 228 20 122.37 0.42 595 208 229 21 121.72 0.42 596 208 230 22 121.64 0.39 597 209 212 3 885.26 1.92 598 209 230 21 121.67 0.40 599 211 216 5 852.13 0.14 600 212 216 4 834.67 8.88 601 212 217 5 808.87 0.01 602 212 220 8 418.10 0.17 603 212 229 17 122.47 0.41 604 212 231 19 122.32 0.43 605 212 234 22 103.13 8.92 606 212 243 31 50.45 0.13 607 213 216 3 864.21 0.10 608 213 217 4 834.88 8.76 609 213 234 21 121.68 0.36 610 213 239 26 60.72 0.91 611 213 243 30 50.64 0.20 612 214 217 3 872.23 4.37 613 214 239 25 54.08 1.16 614 214 242 28 50.81 0.09 615 214 243 29 50.55 0.14 616 215 219 4 879.76 0.60 617 216 219 3 865.85 2.70 618 216 220 4 856.28 2.08 619 217 220 3 856.30 2.07 620 217 221 4 841.99 2.47 621 217 229 12 122.62 0.42 622 217 243 26 46.13 0.99 623 217 246 29 33.44 0.30 624 218 221 3 855.90 2.27 625 218 222 4 844.20 3.58 626 218 246 28 33.44 0.30 627 219 222 3 858.89 5.17 628 219 223 4 853.68 3.38 629 220 223 3 866.23 7.04 630 220 226 6 252.85 64.63 631 220 229 9 122.65 0.41 632 221 226 5 393.37 5.63 633 221 229 8 122.66 0.41 634 221 246 25 33.43 0.30 635 223 226 3 857.46 2.69 636 226 229 3 1019.33 2.08 637 229 243 14 41.24 0.72 638 229 246 17 33.43 0.30 639 231 234 3 876.91 0.89 640 231 235 4 820.53 1.05 641 231 240 9 51.34 0.15 642 231 243 12 47.19 0.70 643 232 235 3 864.39 3.10 644 233 236 3 882.83 0.21 645 233 237 4 854.75 0.53 646 234 237 3 869.53 6.86 647 234 239 5 685.54 52.80 648 234 240 6 124.01 0.03 649 234 243 9 88.67 17.70 650 235 240 5 87.45 18.23 651 237 240 3 861.77 1.90 652 238 241 3 864.17 3.19 653 239 242 3 844.87 3.29 654 239 243 4 844.88 3.29 655 240 243 3 840.38 1.02 656 240 244 4 836.38 0.96 657 241 244 3 844.58 1.07 658 241 245 4 836.38 0.96 659 242 245 3 847.58 4.63 660 242 246 4 825.04 6.63 661 243 246 3 827.95 5.12 662 245 248 3 859.86 4.66