BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 226 222 117.64 2.21 2 4 227 223 117.66 2.21 3 4 229 225 118.03 2.02 4 4 230 226 114.53 2.39 5 5 205 200 148.27 15.32 6 5 206 201 145.84 15.56 7 5 207 202 142.10 13.71 8 5 208 203 141.84 13.69 9 5 230 225 118.20 1.96 10 6 37 31 670.96 0.24 11 6 38 32 670.94 0.24 12 6 39 33 670.94 0.24 13 6 208 202 142.12 13.70 14 7 36 29 671.05 0.21 15 7 37 30 671.00 0.24 16 7 38 31 670.98 0.25 17 7 39 32 670.95 0.24 18 7 224 217 118.45 1.89 19 7 230 223 118.44 1.88 20 7 250 243 19.95 0.68 21 8 38 30 671.00 0.24 22 8 39 31 670.95 0.24 23 8 41 33 670.94 0.24 24 8 91 83 158.43 18.52 25 8 164 156 153.93 17.29 26 8 208 200 150.88 16.54 27 8 210 202 142.02 13.64 28 8 230 222 118.55 1.87 29 8 231 223 118.46 1.88 30 8 232 224 118.45 1.88 31 9 38 29 671.07 0.23 32 9 39 30 671.01 0.24 33 9 40 31 670.97 0.25 34 9 41 32 670.97 0.25 35 9 232 223 118.47 1.87 36 9 243 234 51.64 0.65 37 9 247 238 44.87 0.20 38 10 41 31 670.97 0.26 39 10 125 115 157.06 18.56 40 10 210 200 153.54 17.48 41 10 232 222 118.58 1.84 42 10 233 223 118.45 1.88 43 10 234 224 118.45 1.88 44 10 243 233 52.09 0.42 45 11 41 30 671.00 0.24 46 11 42 31 670.97 0.25 47 11 43 32 670.97 0.26 48 11 234 223 118.45 1.88 49 11 238 227 84.56 0.33 50 11 243 232 52.09 0.42 51 12 43 31 670.99 0.24 52 12 66 54 383.57 36.91 53 12 96 84 157.66 18.48 54 12 233 221 120.43 1.26 55 12 234 222 119.23 1.51 56 12 235 223 118.44 1.87 57 12 238 226 90.44 1.77 58 13 16 3 867.84 1.12 59 13 22 9 671.93 0.35 60 13 25 12 671.91 0.35 61 13 26 13 671.94 0.36 62 13 29 16 671.72 0.44 63 13 42 29 671.07 0.23 64 13 43 30 671.01 0.24 65 13 44 31 670.99 0.24 66 13 52 39 603.46 2.87 67 13 66 53 383.94 37.07 68 13 238 225 117.66 1.83 69 13 239 226 86.04 0.36 70 14 45 31 670.99 0.24 71 14 66 52 383.91 37.06 72 14 96 82 158.33 18.46 73 14 98 84 157.93 18.55 74 14 173 159 157.56 18.39 75 14 235 221 120.40 1.25 76 15 45 30 671.00 0.24 77 16 45 29 671.03 0.24 78 16 239 223 118.20 1.98 79 17 22 5 689.83 5.96 80 17 48 31 670.77 0.33 81 18 21 3 849.72 4.17 82 18 22 4 828.83 1.17 83 18 48 30 670.99 0.25 84 19 22 3 863.27 2.62 85 19 23 4 832.05 0.45 86 19 51 32 628.52 1.81 87 20 24 4 879.08 0.30 88 21 24 3 899.91 1.96 89 21 25 4 879.08 0.30 90 21 239 218 118.05 2.02 91 22 25 3 868.24 5.11 92 22 26 4 859.77 2.50 93 22 48 26 670.62 0.38 94 22 51 29 612.21 1.90 95 22 239 217 117.18 2.14 96 23 26 3 878.29 2.09 97 23 27 4 855.18 1.42 98 23 51 28 615.63 0.23 99 23 55 32 584.48 0.86 100 24 27 3 865.81 3.90 101 24 28 4 855.18 1.42 102 25 28 3 855.19 1.42 103 25 29 4 855.18 1.42 104 25 239 214 98.23 4.49 105 26 29 3 863.05 3.10 106 26 30 4 853.44 0.54 107 26 51 25 608.94 0.26 108 26 52 26 601.79 3.66 109 26 55 29 520.53 27.55 110 26 56 30 445.14 7.17 111 27 30 3 862.98 3.14 112 27 31 4 853.36 0.49 113 28 31 3 853.25 0.44 114 28 32 4 853.23 0.43 115 28 244 216 55.17 1.09 116 29 32 3 854.13 0.03 117 29 42 13 671.13 0.19 118 29 56 27 385.17 37.13 119 29 58 29 384.74 37.08 120 29 61 32 384.56 37.10 121 30 55 25 512.13 23.35 122 30 56 26 385.11 37.16 123 30 57 27 384.94 37.11 124 30 58 28 384.75 37.08 125 32 58 26 384.74 37.08 126 32 244 212 51.62 0.60 127 34 38 4 869.89 7.72 128 34 40 6 674.08 0.36 129 34 60 26 384.48 37.12 130 34 61 27 384.48 37.12 131 34 244 210 51.59 0.61 132 34 247 213 48.34 0.41 133 34 248 214 47.08 0.10 134 36 60 24 384.40 37.11 135 36 247 211 49.82 0.34 136 36 248 212 47.13 0.07 137 36 250 214 37.13 0.43 138 36 251 215 22.14 1.78 139 38 60 22 384.25 37.11 140 38 247 209 45.86 0.68 141 38 250 212 29.05 0.91 142 39 60 21 384.24 37.12 143 39 61 22 384.24 37.12 144 39 62 23 384.23 37.12 145 39 91 52 158.26 18.61 146 40 60 20 384.26 37.11 147 40 61 21 384.25 37.11 148 40 62 22 384.23 37.12 149 40 247 207 45.37 0.43 150 41 62 21 384.23 37.11 151 41 64 23 384.06 37.12 152 41 91 50 158.47 18.51 153 42 52 10 573.56 1.10 154 42 56 14 384.73 37.08 155 42 61 19 384.38 37.11 156 42 62 20 384.28 37.11 157 42 63 21 384.22 37.11 158 42 64 22 384.17 37.09 159 43 64 21 384.14 37.09 160 43 66 23 383.50 36.89 161 43 93 50 158.73 18.63 162 44 48 4 853.34 1.94 163 44 49 5 750.52 33.19 164 44 52 8 627.58 22.35 165 44 63 19 384.37 37.12 166 44 64 20 384.31 37.12 167 44 66 22 384.06 37.12 168 45 48 3 863.93 3.33 169 45 49 4 855.23 1.01 170 45 66 21 384.24 37.11 171 46 49 3 885.28 2.96 172 46 50 4 889.12 3.62 173 46 65 19 384.36 37.12 174 46 67 21 384.29 37.12 175 46 79 33 178.72 17.95 176 46 84 38 176.93 17.32 177 47 50 3 924.48 0.28 178 48 51 3 878.86 0.03 179 48 52 4 853.57 1.41 180 49 52 3 855.33 1.65 181 49 53 4 852.11 0.72 182 49 63 14 384.52 37.12 183 49 65 16 384.38 37.11 184 49 84 35 176.75 17.30 185 49 87 38 174.81 16.37 186 49 89 40 165.47 20.88 187 50 53 3 878.57 1.97 188 50 54 4 849.14 3.62 189 50 87 37 174.72 16.41 190 51 54 3 866.58 7.96 191 51 55 4 852.97 1.72 192 52 55 3 866.02 8.24 193 52 56 4 852.97 1.72 194 52 63 11 384.56 37.09 195 53 63 10 384.59 37.09 196 53 87 34 174.69 16.41 197 53 88 35 171.34 17.98 198 53 89 36 160.17 18.66 199 56 61 5 629.19 32.91 200 56 63 7 384.57 37.09 201 58 61 3 859.86 3.25 202 61 243 182 52.09 0.42 203 61 247 186 47.31 0.03 204 62 91 29 158.15 18.66 205 63 89 26 160.09 18.57 206 63 91 28 158.32 18.62 207 64 89 25 160.50 18.67 208 64 91 27 159.25 18.50 209 64 92 28 159.25 18.51 210 65 84 19 173.18 17.31 211 65 89 24 160.80 18.55 212 65 91 26 160.09 18.57 213 65 92 27 159.76 18.48 214 65 93 28 159.81 18.51 215 66 93 27 160.30 18.47 216 67 84 17 178.87 18.05 217 67 92 25 160.81 18.54 218 67 93 26 160.76 18.52 219 67 94 27 160.78 18.53 220 67 99 32 161.02 18.41 221 67 105 38 166.12 20.55 222 67 113 46 170.23 16.38 223 68 79 11 178.91 18.04 224 68 80 12 178.90 18.04 225 68 81 13 178.89 18.05 226 68 84 16 178.89 18.04 227 68 92 24 161.49 18.30 228 68 94 26 161.07 18.42 229 68 113 45 174.09 16.57 230 69 75 6 182.13 18.09 231 69 78 9 179.41 18.05 232 69 79 10 179.34 18.09 233 69 80 11 178.92 18.04 234 69 81 12 178.92 18.04 235 69 113 44 174.50 16.43 236 70 81 11 179.04 17.98 237 70 112 42 174.74 16.34 238 70 113 43 174.67 16.37 239 70 116 46 174.73 16.34 240 71 75 4 896.22 4.23 241 71 80 9 179.41 18.05 242 72 75 3 948.12 0.52 243 72 80 8 179.47 18.04 244 72 82 10 179.49 18.05 245 72 83 11 179.50 18.05 246 75 78 3 883.91 4.68 247 79 83 4 849.23 0.64 248 79 84 5 849.24 0.61 249 80 83 3 863.72 0.99 250 80 84 4 853.39 2.68 251 81 84 3 874.72 3.59 252 81 85 4 853.39 2.68 253 81 92 11 193.90 13.62 254 81 113 32 268.56 23.11 255 81 116 35 326.55 40.77 256 81 117 36 198.44 14.75 257 81 122 41 403.94 17.72 258 82 85 3 853.40 2.68 259 82 86 4 853.38 2.68 260 83 86 3 852.78 1.87 261 83 87 4 862.40 6.03 262 84 87 3 865.49 4.49 263 84 88 4 862.31 6.08 264 84 89 5 703.24 64.10 265 84 122 38 480.43 8.61 266 85 88 3 862.31 6.08 267 85 89 4 829.00 1.23 268 85 120 35 486.65 5.50 269 85 122 37 479.78 8.94 270 89 122 33 546.91 0.68 271 90 122 32 546.90 0.68 272 90 123 33 546.88 0.67 273 91 122 31 546.93 0.68 274 91 123 32 546.88 0.67 275 91 125 34 546.88 0.69 276 91 164 73 546.89 0.68 277 92 117 25 547.02 0.67 278 92 122 30 547.00 0.66 279 92 123 31 546.99 0.66 280 92 124 32 546.90 0.68 281 92 125 33 546.89 0.68 282 93 96 3 938.25 1.75 283 93 125 32 546.90 0.68 284 93 127 34 546.84 0.68 285 94 113 19 547.24 0.67 286 94 114 20 547.02 0.68 287 94 117 23 547.24 0.67 288 94 124 30 546.99 0.67 289 94 125 31 546.94 0.68 290 94 126 32 546.90 0.68 291 94 127 33 546.84 0.68 292 95 100 5 829.68 3.35 293 95 105 10 547.23 0.66 294 95 110 15 547.22 0.66 295 95 127 32 546.88 0.69 296 96 99 3 860.92 2.46 297 96 100 4 846.96 3.31 298 96 127 31 546.90 0.69 299 96 168 72 546.82 0.69 300 96 173 77 487.78 2.71 301 97 100 3 857.14 3.59 302 97 101 4 839.25 7.17 303 97 170 73 536.83 1.23 304 97 173 76 490.71 1.24 305 98 101 3 856.50 3.23 306 98 102 4 829.71 3.36 307 98 103 5 829.70 3.37 308 98 173 75 499.20 0.31 309 99 103 4 820.27 1.34 310 99 104 5 820.25 1.35 311 99 105 6 547.53 0.70 312 100 105 5 547.48 0.67 313 100 107 7 547.48 0.67 314 100 110 10 547.45 0.66 315 100 127 27 547.09 0.68 316 100 147 47 547.11 0.69 317 101 170 69 544.00 0.01 318 104 109 5 836.66 1.55 319 105 109 4 833.68 3.04 320 105 110 5 820.55 2.08 321 105 113 8 818.46 1.28 322 106 109 3 865.23 8.95 323 106 110 4 837.75 1.07 324 107 110 3 867.36 7.88 325 107 111 4 837.77 1.06 326 107 146 39 547.25 0.76 327 107 147 40 547.11 0.69 328 107 150 43 547.56 0.61 329 108 111 3 853.95 1.16 330 108 112 4 848.11 4.11 331 108 154 46 549.72 0.78 332 109 112 3 887.12 2.01 333 109 113 4 853.98 1.18 334 110 113 3 860.46 2.07 335 110 114 4 852.22 2.04 336 110 151 41 547.66 0.61 337 111 114 3 852.24 2.04 338 111 115 4 848.27 4.01 339 111 150 39 547.62 0.62 340 111 151 40 547.58 0.62 341 111 154 43 549.66 0.74 342 112 115 3 860.75 1.28 343 112 116 4 854.00 1.18 344 112 154 42 549.75 0.77 345 113 116 3 871.64 0.16 346 113 117 4 859.70 1.79 347 114 117 3 867.62 2.17 348 114 118 4 859.71 1.79 349 114 124 10 549.69 0.70 350 114 126 12 547.62 0.61 351 114 151 37 549.51 0.77 352 114 155 41 562.63 6.89 353 115 118 3 874.15 1.11 354 115 119 4 854.37 4.45 355 115 154 39 562.56 6.88 356 115 155 40 562.58 6.89 357 116 119 3 865.51 0.51 358 116 120 4 854.35 4.46 359 117 120 3 858.46 6.75 360 117 121 4 838.59 3.43 361 117 122 5 809.76 0.13 362 117 124 7 624.35 34.95 363 118 121 3 868.08 6.32 364 118 122 4 824.18 1.52 365 118 124 6 624.38 34.96 366 118 155 37 706.33 3.39 367 118 159 41 643.59 34.76 368 118 162 44 692.77 1.19 369 118 163 45 634.53 30.30 370 121 162 41 806.40 1.33 371 122 162 40 806.42 1.33 372 123 162 39 806.41 1.33 373 124 162 38 806.49 1.34 374 124 163 39 806.44 1.32 375 124 164 40 806.44 1.32 376 125 164 39 806.44 1.32 377 125 166 41 806.36 1.33 378 125 233 108 170.28 23.70 379 126 151 25 806.63 1.38 380 126 163 37 806.53 1.34 381 126 164 38 806.49 1.34 382 126 165 39 806.47 1.33 383 126 166 40 806.45 1.33 384 127 166 39 806.47 1.34 385 127 233 106 172.02 23.71 386 128 147 19 806.71 1.42 387 128 148 20 806.71 1.42 388 128 165 37 806.54 1.34 389 128 166 38 806.49 1.34 390 128 167 39 806.48 1.34 391 128 168 40 806.47 1.34 392 128 191 63 367.80 18.12 393 129 143 14 806.96 1.39 394 129 144 15 806.92 1.39 395 129 147 18 806.90 1.39 396 129 167 38 806.48 1.34 397 129 168 39 806.48 1.34 398 129 169 40 806.43 1.33 399 130 134 4 833.31 10.26 400 130 135 5 825.99 6.59 401 130 140 10 806.96 1.39 402 130 143 13 806.94 1.39 403 130 144 14 806.93 1.39 404 130 167 37 806.54 1.33 405 130 169 39 806.47 1.34 406 130 170 40 806.46 1.34 407 130 171 41 806.46 1.34 408 131 134 3 854.71 0.40 409 131 135 4 859.77 2.14 410 131 170 39 806.45 1.33 411 131 171 40 806.45 1.33 412 132 135 3 854.09 5.00 413 132 136 4 854.07 5.00 414 132 171 39 806.45 1.33 415 133 136 3 868.40 2.16 416 133 137 4 854.07 5.00 417 134 137 3 863.25 0.42 418 134 138 4 846.88 8.60 419 134 139 5 807.09 1.40 420 134 143 9 807.08 1.40 421 135 138 3 848.88 2.42 422 135 140 5 807.05 1.39 423 135 167 32 806.62 1.33 424 135 171 36 806.48 1.35 425 135 183 48 369.52 17.96 426 135 187 52 369.31 17.96 427 139 142 3 881.88 1.61 428 139 143 4 842.31 1.46 429 140 143 3 875.67 1.50 430 140 144 4 854.71 4.71 431 140 187 47 368.37 18.14 432 141 144 3 872.33 3.18 433 141 145 4 842.34 1.49 434 141 190 49 368.26 18.11 435 142 145 3 870.21 3.04 436 142 146 4 854.62 4.66 437 143 146 3 860.95 2.95 438 143 147 4 854.62 4.66 439 144 147 3 863.19 0.38 440 144 148 4 854.12 4.91 441 144 167 23 806.50 1.34 442 144 187 43 368.26 18.10 443 144 190 46 368.11 18.15 444 144 191 47 367.93 18.13 445 145 148 3 855.12 4.38 446 145 149 4 854.10 4.89 447 145 190 45 368.07 18.16 448 145 194 49 367.80 18.13 449 146 149 3 865.88 0.99 450 146 150 4 854.52 4.69 451 147 150 3 870.11 0.93 452 147 151 4 855.12 4.38 453 148 151 3 874.74 3.25 454 148 152 4 866.01 1.11 455 148 160 12 806.96 1.24 456 148 165 17 806.58 1.31 457 148 191 43 367.81 18.13 458 148 194 46 367.74 18.12 459 148 195 47 367.31 17.98 460 149 152 3 871.60 0.17 461 149 153 4 866.05 1.12 462 149 194 45 367.74 18.12 463 150 153 3 890.65 1.82 464 150 154 4 866.05 1.12 465 151 154 3 874.54 1.30 466 151 155 4 861.60 3.35 467 152 155 3 863.27 2.52 468 152 160 8 807.32 1.39 469 152 194 42 367.73 18.13 470 155 159 4 846.61 0.64 471 155 160 5 833.38 7.25 472 155 163 8 806.94 1.17 473 156 159 3 874.96 1.90 474 156 160 4 869.71 1.73 475 157 160 3 891.93 3.04 476 157 199 42 346.71 22.36 477 157 203 46 346.51 22.34 478 158 161 3 892.75 2.64 479 159 162 3 886.43 0.52 480 159 163 4 865.03 0.62 481 160 163 3 842.34 1.01 482 160 195 35 364.60 17.00 483 160 199 39 346.73 22.35 484 160 203 43 346.25 22.47 485 160 207 47 345.94 22.37 486 163 207 44 345.94 22.38 487 164 207 43 346.04 22.42 488 164 208 44 345.66 22.50 489 164 210 46 345.27 22.52 490 165 191 26 367.69 18.17 491 165 207 42 346.09 22.44 492 165 208 43 345.91 22.40 493 165 209 44 345.29 22.55 494 165 210 45 345.29 22.55 495 166 210 44 345.31 22.54 496 166 233 67 172.02 23.71 497 167 187 20 367.91 18.12 498 167 188 21 367.92 18.12 499 167 191 24 367.75 18.14 500 167 210 43 345.55 22.55 501 167 211 44 345.24 22.55 502 167 212 45 345.17 22.55 503 168 173 5 810.13 2.45 504 168 212 44 345.10 22.53 505 168 213 45 345.00 22.52 506 168 233 65 172.68 23.82 507 169 172 3 861.10 3.30 508 169 173 4 855.88 5.92 509 169 179 10 369.49 17.95 510 169 211 42 345.59 22.56 511 169 212 43 345.43 22.59 512 170 173 3 861.11 3.31 513 171 175 4 840.22 0.12 514 171 177 6 396.15 17.13 515 171 179 8 372.57 16.93 516 171 183 12 369.62 17.91 517 172 175 3 877.96 5.12 518 172 177 5 372.41 16.85 519 172 178 6 372.08 16.69 520 172 179 7 369.63 17.89 521 172 211 39 345.74 22.54 522 172 213 41 344.99 22.53 523 172 214 42 344.99 22.53 524 173 213 40 344.98 22.53 525 175 214 39 345.11 22.55 526 176 214 38 345.35 22.66 527 177 214 37 345.39 22.64 528 178 211 33 346.07 22.46 529 178 213 35 345.47 22.61 530 178 214 36 345.29 22.59 531 178 219 41 308.49 14.48 532 178 223 45 242.00 2.20 533 179 183 4 837.10 0.80 534 179 184 5 688.84 63.82 535 179 211 32 346.07 22.46 536 179 223 44 241.83 2.15 537 180 183 3 867.87 2.67 538 180 184 4 850.75 5.88 539 180 223 43 241.79 2.12 540 181 184 3 865.12 1.30 541 181 185 4 845.19 3.09 542 181 222 41 241.90 2.27 543 181 223 42 241.69 2.17 544 181 226 45 238.40 3.46 545 182 185 3 859.54 4.07 546 182 186 4 850.45 0.43 547 182 226 44 239.08 3.12 548 183 186 3 865.03 1.32 549 183 187 4 865.03 1.32 550 184 187 3 865.03 1.32 551 184 188 4 860.28 3.68 552 184 211 27 346.11 22.44 553 184 223 39 241.40 2.02 554 184 229 45 173.80 24.17 555 185 188 3 865.01 1.31 556 185 189 4 851.27 0.83 557 185 226 41 178.25 26.37 558 185 227 42 174.02 24.22 559 185 228 43 173.74 24.19 560 185 229 44 173.74 24.20 561 186 189 3 866.25 1.91 562 186 190 4 856.02 3.21 563 187 190 3 862.04 0.20 564 187 191 4 846.40 0.17 565 188 191 3 863.26 0.48 566 188 192 4 846.40 0.17 567 188 209 21 346.34 22.40 568 188 210 22 346.34 22.40 569 188 211 23 346.30 22.41 570 188 228 40 173.58 24.14 571 188 229 41 173.50 24.12 572 189 192 3 864.13 0.97 573 189 193 4 846.35 0.17 574 189 228 39 173.57 24.16 575 190 193 3 875.28 4.60 576 190 194 4 844.88 0.57 577 191 194 3 864.91 1.35 578 191 195 4 857.08 5.27 579 191 209 18 346.34 22.41 580 192 195 3 862.25 0.04 581 192 196 4 852.32 4.29 582 192 204 12 346.66 22.36 583 192 207 15 346.55 22.37 584 192 209 17 346.36 22.40 585 192 228 36 173.55 24.16 586 192 230 38 173.49 24.15 587 193 196 3 855.96 5.22 588 193 197 4 852.73 4.98 589 194 197 3 860.98 0.86 590 194 198 4 854.06 4.32 591 195 198 3 860.98 0.86 592 195 199 4 833.68 5.65 593 195 203 8 347.33 22.09 594 195 204 9 346.68 22.36 595 195 207 12 346.55 22.39 596 196 199 3 866.55 2.82 597 196 200 4 525.88 79.94 598 196 201 5 452.03 51.48 599 196 204 8 346.69 22.36 600 199 203 4 849.88 3.57 601 200 203 3 886.28 0.49 602 200 204 4 849.93 3.56 603 201 204 3 864.21 0.81 604 201 205 4 849.92 3.56 605 202 205 3 863.83 0.63 606 202 206 4 849.90 3.55 607 203 206 3 864.06 0.78 608 203 207 4 835.97 3.41 609 204 207 3 862.16 1.47 610 207 230 23 173.28 24.07 611 208 230 22 173.24 24.08 612 209 228 19 173.47 24.09 613 209 229 20 173.31 24.07 614 209 230 21 173.29 24.06 615 210 230 20 173.09 23.96 616 210 231 21 172.85 23.89 617 210 232 22 172.71 23.84 618 210 233 23 172.71 23.84 619 211 215 4 883.43 3.84 620 211 223 12 185.03 29.92 621 211 229 18 173.41 24.07 622 211 231 20 172.94 23.88 623 211 232 21 172.72 23.84 624 211 233 22 172.72 23.84 625 212 215 3 896.73 1.75 626 212 233 21 172.68 23.82 627 214 219 5 849.00 7.71 628 215 219 4 830.57 1.51 629 215 220 5 695.76 4.46 630 215 223 8 375.63 16.51 631 215 232 17 173.00 23.86 632 215 234 19 172.80 23.81 633 215 237 22 172.24 23.60 634 215 246 31 50.87 0.62 635 216 219 3 866.51 1.04 636 216 220 4 771.83 26.48 637 216 237 21 172.28 23.62 638 216 242 26 114.44 28.59 639 216 246 30 51.34 0.71 640 217 220 3 865.94 1.34 641 217 242 25 114.44 28.59 642 217 245 28 51.57 0.62 643 217 246 29 51.34 0.71 644 219 223 4 854.83 4.83 645 220 223 3 860.47 2.08 646 220 224 4 854.83 4.83 647 220 232 12 173.55 24.02 648 220 246 26 49.72 0.48 649 220 249 29 39.66 0.21 650 221 224 3 869.00 3.80 651 221 225 4 854.83 4.83 652 221 249 28 38.20 0.93 653 221 251 30 38.20 0.94 654 222 225 3 871.16 5.01 655 222 226 4 860.56 1.97 656 223 226 3 874.07 3.23 657 223 229 6 174.22 24.11 658 223 232 9 174.00 24.20 659 224 229 5 174.22 24.11 660 224 230 6 174.21 24.11 661 224 231 7 174.18 24.12 662 224 232 8 174.00 24.19 663 224 249 25 38.17 0.92 664 224 250 26 28.89 2.68 665 226 229 3 860.22 0.35 666 229 232 3 1051.52 2.88 667 231 250 19 19.94 0.69 668 232 246 14 47.38 0.06 669 232 250 18 28.50 0.03 670 234 237 3 900.19 1.33 671 234 238 4 836.10 1.10 672 234 243 9 52.09 0.42 673 234 246 12 49.95 0.36 674 235 238 3 867.73 0.35 675 236 239 3 892.39 2.49 676 236 240 4 873.35 3.42 677 237 240 3 867.81 0.65 678 237 242 5 574.16 64.04 679 237 243 6 269.06 71.33 680 237 246 9 269.04 71.33 681 238 243 5 270.23 70.75 682 240 243 3 890.41 1.43 683 241 244 3 890.67 1.29 684 242 245 3 870.77 4.50 685 242 246 4 870.59 4.58 686 243 246 3 870.58 4.60 687 243 247 4 855.00 3.37 688 244 247 3 873.29 3.25 689 244 248 4 855.03 3.36 690 245 248 3 860.57 0.59 691 245 249 4 855.39 3.18 692 246 249 3 855.41 3.17 693 246 250 4 863.53 0.83 694 247 250 3 874.33 4.58 695 248 251 3 877.06 1.44