BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 41 40 73.92 0.71 2 1 42 41 75.73 0.07 3 1 92 91 72.85 1.15 4 1 95 94 68.70 0.31 5 2 25 23 87.41 0.47 6 2 26 24 87.42 0.48 7 2 28 26 88.03 0.44 8 2 39 37 87.50 0.43 9 2 40 38 85.81 0.46 10 2 41 39 77.84 0.85 11 2 42 40 77.86 0.83 12 3 20 17 87.70 0.41 13 3 25 22 87.70 0.41 14 3 26 23 87.86 0.37 15 3 27 24 87.86 0.37 16 3 28 25 88.17 0.51 17 3 40 37 86.84 0.76 18 3 42 39 81.17 1.32 19 4 28 24 88.17 0.51 20 4 30 26 88.28 0.48 21 4 37 33 87.64 0.36 22 4 40 36 87.56 0.41 23 4 42 38 86.85 0.75 24 4 43 39 86.84 0.75 25 4 44 40 87.48 0.43 26 5 16 11 87.86 0.41 27 5 20 15 87.94 0.40 28 5 28 23 88.27 0.50 29 5 29 24 88.27 0.49 30 5 30 25 88.31 0.48 31 5 44 39 87.64 0.35 32 5 46 41 87.55 0.41 33 6 30 24 88.31 0.48 34 6 33 27 88.28 0.48 35 6 37 31 88.29 0.48 36 6 43 37 87.83 0.39 37 6 44 38 87.73 0.39 38 6 45 39 87.65 0.36 39 6 46 40 87.65 0.36 40 6 55 49 87.80 0.40 41 7 10 3 916.01 2.16 42 7 13 6 250.06 63.58 43 7 29 22 90.64 0.17 44 7 30 23 89.49 0.49 45 7 31 24 89.08 0.70 46 7 46 39 87.73 0.39 47 8 46 38 88.06 0.46 48 8 51 43 88.11 0.42 49 8 54 46 88.91 0.74 50 9 13 4 854.34 0.77 51 10 13 3 854.34 0.77 52 12 15 3 846.04 2.51 53 12 16 4 831.97 0.76 54 12 81 69 93.17 0.84 55 13 16 3 841.07 0.04 56 13 17 4 836.38 1.45 57 13 29 16 114.64 1.21 58 14 17 3 841.58 0.22 59 14 18 4 836.57 1.41 60 15 18 3 841.52 0.25 61 15 19 4 836.42 1.48 62 15 81 66 120.86 1.56 63 15 84 69 120.87 1.56 64 15 85 70 120.88 1.56 65 16 19 3 836.39 1.50 66 16 20 4 836.38 1.50 67 16 44 28 120.86 1.56 68 16 46 30 120.89 1.56 69 16 68 52 120.88 1.56 70 16 84 68 120.88 1.56 71 17 20 3 841.66 0.18 72 17 21 4 836.38 1.50 73 17 27 10 122.79 0.84 74 17 29 12 122.78 0.82 75 18 21 3 839.43 0.86 76 18 22 4 829.12 2.03 77 19 22 3 838.73 0.52 78 19 23 4 835.53 1.08 79 19 84 65 122.72 0.85 80 19 85 66 122.64 0.82 81 19 88 69 122.63 0.82 82 20 23 3 830.85 3.21 83 20 24 4 820.39 2.02 84 20 25 5 765.00 6.33 85 20 27 7 418.11 146.76 86 20 42 22 122.76 0.83 87 20 44 24 122.78 0.83 88 20 88 68 122.67 0.81 89 21 24 3 834.96 3.17 90 22 85 63 122.81 0.83 91 22 89 67 122.73 0.81 92 23 88 65 122.80 0.83 93 23 89 66 122.70 0.80 94 23 92 69 122.75 0.82 95 25 42 17 122.78 0.80 96 25 92 67 122.72 0.78 97 28 40 12 123.12 0.66 98 30 34 4 837.75 2.03 99 30 36 6 778.09 6.79 100 30 37 7 528.14 95.22 101 30 40 10 137.69 2.87 102 33 37 4 850.04 1.54 103 33 54 21 137.32 3.13 104 33 55 22 135.47 2.21 105 33 58 25 164.80 2.02 106 34 37 3 868.57 2.18 107 34 58 24 167.86 0.58 108 35 38 3 877.58 0.72 109 35 58 23 176.34 4.77 110 36 39 3 850.04 2.28 111 36 40 4 849.18 2.01 112 37 40 3 863.28 1.19 113 37 43 6 185.80 1.08 114 37 55 18 191.88 2.75 115 37 59 22 192.40 2.94 116 37 63 26 187.56 0.64 117 38 59 21 193.61 2.75 118 38 60 22 201.09 0.99 119 38 61 23 192.53 3.00 120 38 63 25 189.25 1.41 121 40 63 23 201.61 0.86 122 41 63 22 201.74 0.92 123 41 64 23 201.80 0.88 124 41 95 54 201.29 0.91 125 42 63 21 201.81 0.88 126 42 64 22 201.72 0.92 127 42 92 50 201.77 0.79 128 42 95 53 201.52 0.91 129 42 232 190 201.61 0.88 130 43 55 12 203.44 0.36 131 43 59 16 202.68 0.70 132 43 63 20 201.94 0.83 133 43 64 21 201.92 0.83 134 43 65 22 201.92 0.83 135 43 66 23 201.93 0.84 136 44 66 22 201.96 0.83 137 44 68 24 204.77 0.35 138 45 51 6 770.06 2.60 139 45 52 7 530.05 105.34 140 45 55 10 207.24 0.84 141 45 65 20 202.63 0.66 142 45 66 21 202.63 0.66 143 45 67 22 202.63 0.66 144 45 68 23 204.77 0.35 145 46 51 5 808.96 0.56 146 46 68 22 210.65 0.23 147 46 70 24 222.13 2.74 148 47 51 4 809.15 0.46 149 47 68 21 222.05 2.74 150 47 69 22 222.08 2.75 151 47 70 23 222.14 2.74 152 47 71 24 223.59 2.94 153 48 51 3 846.09 2.62 154 48 52 4 839.95 0.53 155 48 69 21 222.18 2.73 156 49 52 3 860.00 4.33 157 49 53 4 834.27 3.29 158 49 73 24 235.76 0.16 159 49 74 25 232.27 1.10 160 49 75 26 232.73 1.34 161 49 76 27 223.44 2.12 162 50 53 3 840.26 0.34 163 50 54 4 840.27 0.35 164 51 54 3 840.30 0.35 165 51 55 4 840.27 0.35 166 52 55 3 840.28 0.35 167 52 56 4 839.93 0.53 168 52 65 13 235.92 0.40 169 52 67 15 235.92 0.12 170 52 76 24 236.22 0.26 171 52 77 25 236.05 0.18 172 52 231 179 235.55 0.20 173 53 56 3 852.20 4.16 174 53 57 4 841.57 0.96 175 53 76 23 237.49 0.33 176 53 114 61 238.50 0.61 177 53 115 62 239.64 0.90 178 53 209 156 240.37 1.21 179 54 57 3 849.85 3.36 180 54 211 157 256.69 0.77 181 55 58 3 871.60 0.83 182 55 59 4 841.57 0.96 183 55 65 10 239.03 0.55 184 56 59 3 845.00 0.94 185 56 62 6 807.09 10.24 186 56 65 9 240.35 1.20 187 56 102 46 246.24 0.64 188 56 114 58 245.41 1.02 189 56 229 173 243.47 0.19 190 56 231 175 243.45 0.06 191 57 112 55 263.14 1.79 192 57 211 154 278.04 2.85 193 57 212 155 278.17 2.89 194 59 62 3 857.58 7.30 195 59 63 4 861.72 5.23 196 59 65 6 838.49 0.58 197 59 102 43 277.93 2.91 198 59 112 53 278.18 2.94 199 60 102 42 278.17 2.94 200 60 112 52 278.24 2.91 201 60 227 167 276.55 2.54 202 62 65 3 1011.72 0.61 203 62 102 40 278.26 2.91 204 62 228 166 278.00 2.90 205 62 229 167 277.73 2.99 206 62 230 168 277.20 2.80 207 63 230 167 277.76 3.01 208 64 95 31 277.98 2.91 209 64 97 33 277.69 3.03 210 64 230 166 277.76 3.01 211 64 231 167 277.71 3.02 212 64 232 168 277.25 2.81 213 65 78 13 320.76 15.96 214 65 230 165 278.12 2.93 215 65 231 166 277.78 3.00 216 65 232 167 277.72 3.02 217 66 78 12 320.76 15.96 218 66 79 13 320.76 15.95 219 66 84 18 288.32 7.53 220 66 87 21 278.61 3.00 221 66 88 22 278.51 2.97 222 66 91 25 278.45 2.95 223 66 232 166 278.00 2.88 224 67 77 10 320.78 15.96 225 67 78 11 320.77 15.95 226 67 79 12 320.76 15.95 227 68 77 9 320.81 15.96 228 68 79 11 320.76 15.95 229 68 80 12 320.76 15.96 230 68 81 13 320.76 15.96 231 68 84 16 293.94 10.06 232 69 72 3 928.44 4.83 233 69 73 4 841.85 0.58 234 69 74 5 323.93 14.42 235 69 77 8 320.82 15.96 236 69 79 10 320.81 15.96 237 69 80 11 320.77 15.95 238 69 81 12 320.77 15.95 239 72 249 177 18.40 0.75 240 73 244 171 36.29 0.47 241 73 247 174 25.34 0.80 242 73 248 175 20.29 0.20 243 74 77 3 794.58 1.63 244 74 244 170 36.29 0.47 245 75 156 81 279.31 2.98 246 75 241 166 150.60 2.93 247 75 244 169 36.71 0.70 248 75 245 170 36.27 0.48 249 76 114 38 279.26 2.95 250 76 153 77 279.25 2.96 251 77 234 157 277.66 3.04 252 78 231 153 278.48 2.96 253 78 232 154 278.35 2.92 254 78 234 156 277.33 2.89 255 79 83 4 814.27 11.41 256 79 84 5 353.49 1.56 257 79 87 8 306.80 3.29 258 80 83 3 853.37 4.20 259 80 84 4 797.84 6.95 260 81 84 3 842.46 0.83 261 83 86 3 842.81 0.27 262 83 87 4 838.08 0.32 263 84 87 3 839.86 1.21 264 84 88 4 834.28 1.58 265 85 88 3 834.68 1.43 266 85 89 4 838.11 0.30 267 86 89 3 853.62 1.81 268 86 90 4 840.50 1.51 269 87 90 3 843.71 0.10 270 87 91 4 834.46 1.49 271 88 91 3 839.08 2.19 272 88 92 4 834.45 1.48 273 89 92 3 848.39 2.48 274 89 93 4 825.09 2.09 275 90 93 3 829.00 1.11 276 90 94 4 824.82 2.23 277 91 94 3 841.18 6.08 278 91 95 4 806.36 6.71 279 91 232 141 279.23 3.21 280 94 235 141 281.36 2.96 281 95 232 137 288.75 1.34 282 96 232 136 288.77 1.34 283 96 233 137 288.74 1.33 284 97 230 133 290.12 1.58 285 97 231 134 289.66 1.59 286 97 232 135 288.78 1.33 287 97 233 136 288.77 1.34 288 98 170 72 497.75 1.35 289 98 230 132 294.51 0.40 290 98 231 133 289.88 1.66 291 98 233 135 288.81 1.32 292 98 234 136 288.82 1.32 293 98 237 139 288.42 1.18 294 99 228 129 295.94 0.88 295 99 229 130 295.75 0.92 296 99 230 131 294.59 0.39 297 100 114 14 496.63 1.16 298 100 153 53 496.90 1.03 299 100 169 69 497.81 1.39 300 100 170 70 497.84 1.38 301 100 228 128 296.22 0.87 302 100 229 129 295.87 0.89 303 100 231 131 295.74 0.93 304 100 237 137 253.24 16.50 305 101 114 13 496.90 1.02 306 101 152 51 497.79 1.36 307 101 172 71 497.80 1.36 308 101 193 92 497.74 1.36 309 101 224 123 363.62 2.90 310 101 228 127 297.72 0.47 311 102 112 10 497.37 1.27 312 102 113 11 496.88 1.03 313 102 114 12 497.28 1.22 314 102 225 123 359.83 4.78 315 102 227 125 308.51 3.33 316 102 228 126 298.99 0.08 317 102 229 127 298.97 0.07 318 103 112 9 497.47 1.27 319 103 113 10 497.36 1.23 320 103 117 14 496.92 1.02 321 103 151 48 497.82 1.37 322 103 174 71 497.85 1.34 323 103 193 90 497.86 1.32 324 103 222 119 406.60 0.13 325 103 223 120 404.67 0.01 326 103 224 121 376.32 3.40 327 103 225 122 363.62 2.90 328 104 111 7 497.76 1.41 329 104 112 8 497.69 1.38 330 104 222 118 406.60 0.13 331 104 223 119 406.56 0.12 332 104 225 121 384.39 7.44 333 105 108 3 837.66 2.60 334 105 109 4 788.74 3.63 335 105 117 12 497.69 1.32 336 105 206 101 497.28 1.21 337 105 220 115 416.84 4.46 338 105 221 116 416.72 4.46 339 105 222 117 408.30 0.71 340 106 109 3 827.51 0.58 341 106 220 114 416.84 4.48 342 106 221 115 416.78 4.47 343 107 206 99 497.67 1.31 344 107 214 107 497.30 1.26 345 107 216 109 495.93 1.10 346 107 220 113 416.88 4.46 347 108 111 3 838.03 2.46 348 108 206 98 497.67 1.33 349 108 208 100 497.78 1.30 350 108 212 104 497.74 1.30 351 108 213 105 497.66 1.33 352 108 214 106 497.40 1.27 353 111 208 97 497.86 1.31 354 111 212 101 497.79 1.28 355 112 225 113 363.69 2.91 356 112 227 115 359.69 4.82 357 113 116 3 860.30 3.21 358 113 117 4 808.45 8.91 359 113 208 95 498.04 1.34 360 113 209 96 511.87 0.12 361 114 151 37 638.78 27.34 362 114 153 39 638.79 27.34 363 114 209 95 539.33 0.48 364 114 229 115 298.94 0.07 365 115 150 35 638.83 27.32 366 115 151 36 638.79 27.34 367 115 153 38 638.82 27.36 368 115 154 39 638.78 27.34 369 115 209 94 539.37 0.48 370 116 148 32 638.82 27.28 371 116 149 33 638.87 27.30 372 116 150 34 638.83 27.32 373 116 158 42 638.97 27.27 374 116 208 92 539.60 0.52 375 116 209 93 539.49 0.54 376 117 148 31 638.88 27.31 377 117 149 32 638.86 27.32 378 117 206 89 540.02 0.56 379 117 207 90 539.85 0.48 380 117 208 91 539.60 0.52 381 117 222 105 406.61 0.13 382 118 147 29 638.98 27.27 383 118 148 30 638.88 27.32 384 118 158 40 639.39 27.07 385 118 206 88 540.04 0.56 386 118 207 89 539.87 0.48 387 119 128 9 639.71 26.93 388 119 146 27 639.52 27.00 389 119 147 28 639.28 27.12 390 119 199 80 571.64 2.97 391 119 201 82 570.72 3.23 392 119 204 85 540.44 0.54 393 119 205 86 540.24 0.56 394 119 206 87 540.05 0.56 395 119 216 97 539.39 0.46 396 119 220 101 422.51 1.80 397 120 128 8 639.71 26.93 398 120 145 25 639.63 26.96 399 120 146 26 639.53 27.00 400 120 158 38 639.61 27.00 401 120 179 59 639.62 26.96 402 120 204 84 540.44 0.54 403 120 205 85 540.31 0.53 404 120 207 87 540.21 0.57 405 121 126 5 643.49 28.80 406 121 128 7 639.71 26.93 407 121 145 24 639.69 26.94 408 121 205 84 540.47 0.54 409 122 203 81 569.76 3.22 410 122 204 82 554.46 3.68 411 122 205 83 545.00 0.61 412 122 215 93 539.75 0.46 413 122 216 94 539.75 0.46 414 122 217 95 539.72 0.46 415 123 126 3 854.19 9.30 416 123 203 80 571.56 2.94 417 126 203 77 571.65 2.94 418 127 143 16 648.76 22.46 419 127 144 17 648.74 22.48 420 127 182 55 648.72 22.44 421 128 143 15 648.82 22.45 422 128 144 16 648.77 22.46 423 128 146 18 648.80 22.50 424 128 201 73 573.39 3.08 425 128 202 74 571.59 2.95 426 128 203 75 571.54 2.95 427 128 204 76 570.55 3.27 428 129 141 12 649.07 22.36 429 129 142 13 649.02 22.37 430 129 143 14 648.95 22.39 431 129 201 72 573.69 3.23 432 130 141 11 649.08 22.36 433 130 142 12 649.06 22.36 434 130 144 14 649.07 22.37 435 130 147 17 648.87 22.51 436 130 199 69 576.62 2.73 437 130 200 70 576.46 2.69 438 130 201 71 573.80 3.19 439 131 140 9 649.22 22.36 440 131 141 10 649.08 22.36 441 131 199 68 576.59 2.71 442 131 200 69 576.47 2.70 443 132 138 6 650.02 22.43 444 132 139 7 649.49 22.30 445 132 140 8 649.26 22.38 446 132 147 15 649.16 22.41 447 132 176 44 649.12 22.57 448 132 197 65 581.01 1.75 449 132 198 66 578.30 3.00 450 132 199 67 576.82 2.83 451 133 137 4 733.77 1.57 452 133 138 5 650.03 22.43 453 133 139 6 649.94 22.47 454 133 197 64 581.02 1.76 455 133 198 65 579.84 2.30 456 133 200 67 579.34 2.49 457 133 219 86 520.19 1.14 458 134 137 3 831.28 0.03 459 134 139 5 716.15 10.40 460 134 176 42 649.33 22.55 461 134 191 57 592.05 5.88 462 134 195 61 582.89 1.51 463 134 196 62 582.75 1.54 464 134 197 63 581.02 1.76 465 135 195 60 582.90 1.51 466 135 196 61 582.82 1.53 467 136 198 62 582.67 1.49 468 139 189 50 592.48 5.84 469 140 183 43 712.94 9.27 470 140 189 49 592.50 5.83 471 141 183 42 712.96 9.26 472 142 178 36 700.72 2.96 473 142 181 39 713.01 9.23 474 142 183 41 712.94 9.26 475 142 186 44 689.96 20.43 476 143 181 38 713.04 9.23 477 143 182 39 713.01 9.23 478 144 178 34 713.18 9.12 479 144 179 35 713.37 9.29 480 144 180 36 713.33 9.30 481 144 181 37 713.09 9.21 482 145 179 34 713.50 9.32 483 145 180 35 713.37 9.29 484 146 178 32 713.69 9.23 485 146 179 33 713.50 9.32 486 147 176 29 715.31 9.15 487 147 177 30 714.00 9.33 488 147 178 31 713.70 9.23 489 148 158 10 718.36 8.95 490 148 159 11 718.30 8.94 491 148 162 14 718.32 8.93 492 148 175 27 715.54 9.15 493 148 176 28 715.32 9.15 494 148 177 29 714.08 9.29 495 148 179 31 713.96 9.30 496 149 174 25 715.71 9.12 497 149 175 26 715.56 9.14 498 149 176 27 715.35 9.17 499 149 197 48 576.49 2.73 500 149 199 50 571.53 2.95 501 149 222 73 406.61 0.13 502 150 154 4 758.40 9.04 503 150 159 9 718.49 9.00 504 150 174 24 716.08 9.01 505 150 175 25 715.70 9.11 506 151 154 3 826.68 0.67 507 151 155 4 814.28 5.74 508 151 173 22 716.57 9.03 509 151 175 24 716.10 9.02 510 151 193 42 654.45 38.48 511 152 155 3 827.11 0.46 512 152 167 15 717.94 8.89 513 152 168 16 717.90 8.91 514 152 169 17 717.89 8.91 515 152 171 19 717.50 8.76 516 152 172 20 716.81 9.10 517 152 173 21 716.57 9.03 518 152 175 23 716.57 9.03 519 153 156 3 878.59 0.94 520 153 169 16 717.90 8.91 521 154 157 3 832.44 2.21 522 154 159 5 780.71 1.06 523 155 159 4 780.72 1.07 524 155 160 5 777.35 2.18 525 155 165 10 718.45 9.00 526 155 166 11 718.39 9.00 527 155 167 12 718.35 8.97 528 155 175 20 716.76 9.18 529 156 160 4 798.68 1.59 530 156 166 10 718.43 9.01 531 156 241 85 165.86 2.67 532 156 245 89 36.81 0.65 533 156 248 92 33.61 0.83 534 157 160 3 825.33 0.04 535 157 161 4 829.31 2.02 536 158 161 3 829.82 1.77 537 158 162 4 825.00 0.13 538 158 179 21 715.53 8.63 539 159 162 3 840.64 3.65 540 159 163 4 825.00 0.13 541 159 164 5 779.43 0.89 542 159 175 16 716.85 9.16 543 159 177 18 716.69 9.14 544 160 163 3 833.60 3.50 545 160 164 4 786.53 4.50 546 160 166 6 731.10 3.00 547 160 245 85 39.59 0.50 548 160 248 88 35.86 0.69 549 162 177 15 716.82 9.15 550 162 178 16 716.74 9.16 551 162 179 17 715.48 8.61 552 162 186 24 656.43 37.82 553 163 177 14 716.89 9.12 554 163 188 25 656.12 37.93 555 164 177 13 716.81 9.13 556 164 188 24 656.09 37.95 557 165 175 10 717.01 9.12 558 165 188 23 656.13 37.96 559 165 190 25 656.07 37.95 560 166 241 75 181.20 5.37 561 166 245 79 89.08 24.23 562 167 171 4 817.22 3.72 563 167 173 6 719.97 8.53 564 167 175 8 716.94 9.10 565 168 171 3 847.38 1.40 566 168 238 70 251.58 17.04 567 168 241 73 170.96 0.24 568 169 237 68 252.03 17.11 569 169 241 72 170.93 0.24 570 170 237 67 265.32 10.63 571 170 238 68 252.03 17.11 572 172 193 21 655.51 38.18 573 172 224 52 400.43 2.03 574 173 190 17 655.97 38.02 575 173 191 18 655.90 38.02 576 173 192 19 655.86 37.99 577 173 193 20 655.43 38.15 578 174 190 16 655.95 38.00 579 174 191 17 655.88 38.00 580 174 193 19 655.27 38.07 581 174 195 21 647.03 34.76 582 174 197 23 613.98 19.79 583 174 222 48 414.88 3.78 584 175 189 14 655.96 38.01 585 175 190 15 655.90 37.98 586 176 188 12 656.07 37.95 587 176 189 13 655.90 37.98 588 176 191 15 655.89 37.98 589 176 197 21 579.52 2.39 590 177 186 9 656.36 37.85 591 177 188 11 656.02 37.93 592 178 181 3 828.39 0.76 593 178 186 8 656.38 37.81 594 181 186 5 727.81 9.55 595 183 186 3 901.69 0.39 596 191 195 4 829.25 1.97 597 191 197 6 764.18 11.84 598 192 195 3 845.96 6.25 599 193 224 31 400.44 2.03 600 194 224 30 405.59 0.39 601 196 221 25 416.78 4.49 602 196 222 26 416.50 4.57 603 196 223 27 413.37 3.02 604 197 221 24 416.77 4.48 605 197 222 25 415.63 4.15 606 198 219 21 424.78 2.73 607 198 220 22 417.94 4.01 608 198 221 23 416.78 4.49 609 199 219 20 470.48 20.12 610 199 220 21 418.09 3.96 611 199 222 23 417.94 4.01 612 200 218 18 536.92 0.43 613 200 219 19 472.86 21.32 614 201 204 3 868.81 1.81 615 201 216 15 539.69 0.47 616 201 217 16 539.28 0.47 617 201 218 17 538.06 0.03 618 202 217 15 539.34 0.46 619 202 218 16 539.25 0.45 620 203 217 14 539.42 0.46 621 204 215 11 539.74 0.47 622 204 216 12 539.50 0.50 623 205 213 8 539.78 0.47 624 205 214 9 539.67 0.48 625 205 215 10 539.40 0.46 626 205 216 11 539.39 0.46 627 206 213 7 539.71 0.48 628 206 214 8 539.54 0.52 629 206 216 10 539.39 0.46 630 206 220 14 429.76 1.82 631 207 210 3 826.64 0.30 632 207 211 4 817.34 2.23 633 207 212 5 723.01 8.80 634 207 213 6 539.49 0.42 635 208 211 3 847.94 5.58 636 208 212 4 781.93 11.35 637 216 220 4 858.91 0.92 638 231 237 6 785.95 3.94 639 234 237 3 819.05 1.51 640 236 239 3 840.81 2.48 641 236 240 4 826.75 4.29 642 237 240 3 846.60 0.35 643 237 241 4 817.93 0.01 644 238 241 3 847.93 1.02 645 238 242 4 842.65 3.66 646 240 243 3 849.55 1.17 647 240 244 4 822.87 1.10 648 241 244 3 827.73 0.06 649 241 245 4 822.83 1.12 650 242 245 3 849.43 5.50 651 242 246 4 823.10 0.99 652 243 246 3 844.14 8.14 653 243 247 4 844.01 7.75 654 244 247 3 844.47 7.97 655 244 248 4 824.00 1.43 656 245 248 3 843.18 8.17