BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 174.33 0.69 2 4 204 200 174.16 0.60 3 4 205 201 168.13 0.66 4 4 224 220 119.77 0.95 5 4 227 223 119.53 0.97 6 5 36 31 458.93 40.63 7 5 38 33 459.11 40.66 8 5 205 200 174.14 0.59 9 6 37 31 459.12 40.65 10 6 38 32 459.12 40.66 11 6 221 215 119.62 0.98 12 6 227 221 119.63 0.98 13 6 228 222 119.59 0.98 14 6 229 223 119.60 0.98 15 6 243 237 46.54 0.43 16 6 244 238 41.78 0.78 17 6 246 240 37.59 0.77 18 6 247 241 28.91 0.36 19 7 38 31 459.23 40.70 20 7 40 33 459.16 40.66 21 7 90 83 196.81 0.10 22 7 124 117 185.77 0.35 23 7 161 154 184.40 0.51 24 7 205 198 183.99 0.59 25 7 207 200 170.84 1.05 26 7 227 220 119.82 0.93 27 7 228 221 119.61 0.98 28 7 229 222 119.60 0.98 29 8 37 29 459.31 40.69 30 8 38 30 459.26 40.70 31 8 39 31 459.23 40.70 32 8 40 32 459.23 40.70 33 8 229 221 119.61 0.98 34 8 231 223 119.59 0.98 35 8 240 232 54.23 0.92 36 8 243 235 46.56 0.44 37 8 244 236 39.89 0.16 38 9 40 31 459.15 40.66 39 9 229 220 119.83 0.93 40 9 230 221 119.61 0.98 41 9 231 222 119.59 0.97 42 9 240 231 54.31 0.90 43 10 40 30 459.28 40.70 44 10 41 31 459.16 40.66 45 10 42 32 459.16 40.66 46 10 231 221 119.61 0.98 47 10 235 225 77.00 0.40 48 10 240 230 54.31 0.90 49 11 42 31 459.17 40.67 50 11 64 53 297.09 0.47 51 11 92 81 196.84 0.15 52 11 95 84 196.12 0.00 53 11 230 219 121.42 0.47 54 11 231 220 119.81 0.93 55 11 232 221 119.59 0.98 56 11 235 224 84.27 0.04 57 12 15 3 853.15 2.75 58 12 21 9 460.94 40.37 59 12 24 12 460.06 40.80 60 12 25 13 460.08 40.80 61 12 41 29 459.33 40.69 62 12 42 30 459.28 40.70 63 12 43 31 459.25 40.70 64 12 44 32 459.24 40.70 65 12 51 39 403.84 37.80 66 12 55 43 300.69 0.50 67 12 64 52 297.12 0.48 68 12 235 223 109.10 0.50 69 12 236 224 82.91 0.64 70 13 44 31 459.24 40.70 71 13 64 51 297.12 0.48 72 13 95 82 197.34 0.41 73 13 97 84 196.50 0.01 74 13 170 157 196.36 0.06 75 13 232 219 121.37 0.46 76 15 21 6 812.83 3.06 77 15 44 29 459.33 40.69 78 15 236 221 119.57 0.99 79 16 20 4 814.37 2.29 80 16 21 5 812.83 3.06 81 16 47 31 459.28 40.69 82 17 20 3 846.32 1.42 83 17 21 4 824.36 2.70 84 17 47 30 459.28 40.69 85 18 22 4 814.86 2.15 86 18 47 29 459.30 40.70 87 18 50 32 451.15 38.36 88 19 22 3 847.28 1.97 89 19 23 4 834.33 1.32 90 20 23 3 843.99 3.08 91 20 24 4 834.92 1.02 92 21 24 3 842.70 2.29 93 21 25 4 829.50 1.75 94 21 44 23 459.41 40.68 95 21 47 26 459.26 40.72 96 21 50 29 434.62 43.85 97 22 25 3 846.88 4.65 98 22 26 4 836.30 1.62 99 22 50 28 433.23 44.61 100 23 26 3 845.20 5.50 101 23 27 4 836.30 1.62 102 24 27 3 847.11 4.54 103 24 28 4 831.91 0.58 104 24 235 211 110.77 0.33 105 24 236 212 85.68 0.27 106 24 237 213 77.12 1.11 107 25 28 3 859.41 2.54 108 25 29 4 824.57 4.25 109 25 50 25 419.02 37.50 110 25 51 26 417.56 37.86 111 25 54 29 299.99 0.89 112 25 55 30 299.88 0.88 113 26 29 3 850.75 0.52 114 26 30 4 841.11 3.96 115 27 30 3 850.20 0.55 116 27 31 4 841.11 3.96 117 28 31 3 850.20 0.55 118 28 33 5 824.40 4.17 119 28 41 13 459.49 40.71 120 28 55 27 299.88 0.90 121 28 59 31 298.63 0.85 122 28 240 212 54.38 0.91 123 28 241 213 54.27 0.92 124 28 244 216 53.98 0.86 125 29 54 25 305.89 2.08 126 29 55 26 299.85 0.89 127 29 56 27 299.71 0.88 128 31 241 210 54.23 0.90 129 32 245 213 47.81 0.07 130 33 59 26 297.52 0.33 131 33 241 208 53.98 0.89 132 33 244 211 47.80 0.07 133 33 245 212 46.43 0.51 134 34 37 3 860.91 0.07 135 34 244 210 47.68 0.06 136 34 245 211 44.30 0.44 137 34 248 214 34.06 0.54 138 36 248 212 33.21 0.28 139 37 244 207 45.20 1.07 140 38 90 52 196.88 0.10 141 38 205 167 186.02 0.21 142 39 59 20 297.14 0.43 143 39 60 21 297.14 0.43 144 39 240 201 54.27 0.92 145 39 244 205 40.13 0.29 146 40 60 20 297.14 0.43 147 40 61 21 297.14 0.43 148 40 62 22 297.12 0.43 149 40 90 50 196.88 0.10 150 40 124 84 191.66 0.89 151 41 55 14 298.58 0.76 152 41 59 18 297.61 0.39 153 41 60 19 297.30 0.43 154 41 61 20 297.18 0.45 155 41 62 21 297.16 0.45 156 41 240 199 54.34 0.91 157 42 62 20 297.17 0.45 158 42 64 22 296.95 0.48 159 42 92 50 196.88 0.14 160 43 48 5 460.94 40.11 161 43 51 8 377.42 24.59 162 43 62 19 297.30 0.43 163 43 64 21 297.16 0.45 164 44 47 3 853.12 4.70 165 44 48 4 824.25 3.53 166 44 64 20 297.19 0.47 167 45 48 3 846.82 7.84 168 45 78 33 218.03 1.84 169 47 50 3 855.55 2.79 170 47 51 4 841.70 4.10 171 48 51 3 849.51 0.21 172 48 52 4 841.71 4.11 173 48 61 13 298.04 0.57 174 48 62 14 298.01 0.56 175 48 63 15 297.73 0.48 176 48 78 30 218.04 1.84 177 48 83 35 198.08 0.26 178 48 86 38 198.03 0.24 179 49 52 3 851.19 0.61 180 49 53 4 846.77 1.56 181 49 86 37 192.74 0.35 182 50 53 3 853.28 0.26 183 50 54 4 846.78 1.56 184 51 54 3 858.57 3.08 185 51 55 4 845.11 0.72 186 51 61 10 298.07 0.58 187 52 55 3 860.41 1.19 188 52 56 4 861.40 1.68 189 52 57 5 834.37 1.56 190 52 59 7 541.00 15.62 191 52 60 8 339.93 20.30 192 52 61 9 298.08 0.58 193 52 86 34 197.25 0.24 194 52 87 35 196.21 0.10 195 52 88 36 195.68 0.17 196 55 59 4 755.75 28.78 197 55 61 6 339.89 20.32 198 56 59 3 850.62 0.27 199 60 90 30 197.06 0.18 200 61 88 27 197.33 0.28 201 61 89 28 197.32 0.29 202 61 90 29 197.06 0.18 203 62 90 28 197.08 0.18 204 62 91 29 197.39 0.34 205 62 92 30 197.38 0.34 206 62 124 62 197.07 0.18 207 63 78 15 217.94 1.88 208 63 83 20 198.40 0.18 209 63 90 27 197.46 0.34 210 63 91 28 197.44 0.33 211 63 92 29 197.44 0.33 212 64 92 28 197.53 0.34 213 65 78 13 218.02 1.84 214 65 91 26 198.35 0.21 215 65 92 27 197.74 0.29 216 65 93 28 197.57 0.36 217 65 98 33 198.05 0.56 218 65 104 39 200.72 1.07 219 65 112 47 214.11 1.75 220 66 78 12 218.02 1.84 221 66 79 13 218.00 1.85 222 66 80 14 217.91 1.80 223 66 83 17 217.81 1.80 224 66 91 25 200.39 1.19 225 66 93 27 198.62 0.34 226 66 112 46 214.25 1.70 227 66 116 50 215.97 1.21 228 67 74 7 218.49 1.68 229 67 77 10 218.12 1.84 230 67 78 11 218.08 1.85 231 67 80 13 218.02 1.86 232 67 108 41 214.36 1.68 233 67 112 45 214.36 1.68 234 67 115 48 216.28 1.17 235 68 108 40 215.68 1.05 236 68 111 43 215.95 1.19 237 68 112 44 215.11 1.33 238 68 115 47 216.14 1.23 239 69 74 5 825.90 2.09 240 69 79 10 218.28 1.77 241 69 115 46 216.38 1.20 242 70 79 9 218.27 1.78 243 70 81 11 218.22 1.76 244 70 115 45 217.26 1.28 245 74 77 3 857.66 2.35 246 78 82 4 845.31 2.52 247 78 83 5 837.22 6.57 248 79 82 3 854.71 0.41 249 79 83 4 845.32 2.52 250 80 83 3 845.11 2.63 251 80 84 4 842.77 3.79 252 80 115 35 545.25 4.71 253 80 116 36 543.96 5.26 254 80 119 39 549.49 6.56 255 80 121 41 549.31 6.48 256 81 84 3 856.72 0.01 257 81 85 4 842.75 3.79 258 81 119 38 565.22 1.22 259 82 85 3 842.77 3.80 260 82 86 4 842.75 3.78 261 83 86 3 859.89 1.59 262 83 87 4 840.71 2.76 263 83 88 5 759.13 37.96 264 83 90 7 566.12 0.92 265 83 91 8 565.23 1.22 266 83 121 38 571.24 1.59 267 84 87 3 844.96 0.63 268 84 88 4 820.20 7.44 269 84 119 35 605.88 3.42 270 84 121 37 578.64 5.29 271 88 121 33 665.14 1.37 272 89 121 32 665.12 1.38 273 89 122 33 665.10 1.38 274 90 121 31 665.16 1.38 275 90 122 32 665.12 1.38 276 90 123 33 665.11 1.38 277 90 124 34 665.10 1.38 278 90 159 69 665.16 1.36 279 90 161 71 665.11 1.38 280 91 116 25 665.25 1.38 281 91 121 30 665.24 1.38 282 91 122 31 665.21 1.39 283 91 123 32 665.12 1.38 284 91 124 33 665.11 1.38 285 92 95 3 892.24 1.07 286 92 124 32 665.12 1.38 287 92 126 34 665.08 1.38 288 92 230 138 121.49 0.45 289 93 112 19 665.31 1.38 290 93 113 20 665.24 1.39 291 93 116 23 665.36 1.37 292 93 123 30 665.24 1.38 293 93 124 31 665.19 1.38 294 93 125 32 665.11 1.39 295 93 126 33 665.11 1.39 296 94 99 5 675.89 3.40 297 94 109 15 665.25 1.40 298 94 126 32 665.12 1.38 299 95 98 3 849.53 0.14 300 95 99 4 841.14 4.07 301 95 126 31 665.15 1.39 302 95 165 70 665.11 1.39 303 95 170 75 582.06 1.49 304 95 230 135 121.75 0.53 305 96 99 3 857.84 1.39 306 96 100 4 821.82 5.59 307 96 165 69 665.12 1.39 308 96 167 71 655.43 1.81 309 96 170 74 571.20 3.96 310 97 100 3 852.22 1.42 311 97 101 4 678.81 2.14 312 97 102 5 677.66 2.71 313 97 170 73 625.38 1.08 314 98 102 4 678.79 2.14 315 98 103 5 675.98 3.54 316 98 104 6 666.04 1.21 317 99 103 4 666.03 1.22 318 99 104 5 666.01 1.22 319 99 106 7 666.01 1.21 320 99 109 10 666.00 1.22 321 99 126 27 665.31 1.35 322 99 127 28 665.27 1.36 323 99 146 47 665.32 1.34 324 99 150 51 665.44 1.35 325 100 130 30 665.18 1.38 326 100 167 67 664.89 1.37 327 104 108 4 843.31 0.54 328 104 109 5 834.01 4.12 329 104 112 8 813.33 6.21 330 105 108 3 862.08 0.07 331 105 109 4 843.62 0.39 332 106 109 3 848.24 2.69 333 106 110 4 843.63 0.39 334 106 146 40 665.33 1.36 335 106 149 43 665.49 1.38 336 107 110 3 847.32 1.46 337 107 111 4 843.63 0.38 338 108 111 3 861.30 1.23 339 108 112 4 840.38 1.23 340 109 112 3 848.85 3.21 341 109 113 4 835.47 3.46 342 109 150 41 665.58 1.37 343 110 113 3 855.50 6.54 344 110 114 4 843.80 4.76 345 110 149 39 665.54 1.39 346 110 153 43 665.74 1.33 347 111 114 3 847.15 3.08 348 111 115 4 846.38 3.47 349 112 115 3 858.25 2.46 350 112 116 4 843.82 4.75 351 113 116 3 862.79 4.74 352 113 117 4 839.43 2.56 353 113 123 10 673.44 2.41 354 113 150 37 665.65 1.36 355 113 153 40 673.47 2.40 356 113 154 41 673.86 2.19 357 114 117 3 878.18 4.99 358 114 118 4 839.42 2.56 359 114 153 39 673.40 2.42 360 115 118 3 848.31 2.52 361 115 119 4 830.91 1.25 362 116 119 3 856.66 5.78 363 116 120 4 838.33 2.46 364 116 121 5 752.27 27.73 365 116 123 7 688.80 1.52 366 117 120 3 843.25 0.59 367 117 121 4 824.04 1.21 368 117 123 6 691.25 2.71 369 117 153 36 691.45 2.69 370 117 154 37 691.45 2.69 371 117 159 42 691.47 2.69 372 117 160 43 691.46 2.69 373 122 159 37 791.91 0.85 374 123 159 36 791.95 0.85 375 123 160 37 791.93 0.85 376 123 161 38 791.93 0.85 377 124 161 37 791.93 0.85 378 124 163 39 791.78 0.87 379 124 207 83 436.55 30.59 380 124 230 106 121.04 0.56 381 125 150 25 792.12 0.84 382 125 160 35 791.99 0.85 383 125 161 36 791.97 0.85 384 125 162 37 791.94 0.85 385 125 163 38 791.92 0.85 386 126 150 24 792.26 0.88 387 126 163 37 791.94 0.85 388 126 165 39 791.91 0.85 389 126 230 104 121.57 0.48 390 127 143 16 792.41 0.87 391 127 146 19 792.38 0.86 392 127 147 20 792.38 0.86 393 127 150 23 792.38 0.86 394 127 162 35 792.01 0.85 395 127 163 36 791.96 0.85 396 127 164 37 791.93 0.85 397 127 165 38 791.93 0.85 398 127 188 61 448.37 27.64 399 128 142 14 792.53 0.89 400 128 143 15 792.44 0.88 401 128 146 18 792.46 0.86 402 128 164 36 791.95 0.85 403 128 165 37 791.93 0.85 404 128 166 38 791.89 0.84 405 129 133 4 817.13 3.51 406 129 134 5 802.72 1.47 407 129 139 10 792.54 0.88 408 129 142 13 792.54 0.88 409 129 143 14 792.51 0.89 410 129 164 35 792.02 0.85 411 129 166 37 791.92 0.85 412 129 167 38 791.92 0.85 413 129 168 39 791.91 0.85 414 130 133 3 845.77 1.48 415 130 134 4 835.31 6.71 416 130 167 37 791.92 0.84 417 130 168 38 791.92 0.85 418 131 134 3 845.77 1.49 419 131 135 4 835.32 6.71 420 131 168 37 791.92 0.84 421 132 135 3 854.62 2.37 422 132 136 4 826.28 2.19 423 133 136 3 841.03 3.85 424 133 137 4 841.02 3.86 425 133 138 5 795.34 2.19 426 133 142 9 792.73 0.89 427 134 137 3 842.23 1.32 428 134 139 5 792.74 0.90 429 134 168 34 791.98 0.85 430 134 180 46 449.51 27.67 431 138 141 3 840.22 7.45 432 138 142 4 831.12 2.91 433 139 142 3 860.55 2.71 434 139 143 4 838.00 6.25 435 139 184 45 448.70 27.64 436 140 143 3 872.87 2.08 437 140 144 4 837.88 6.31 438 140 187 47 448.59 27.66 439 141 144 3 850.46 2.64 440 141 145 4 840.79 4.86 441 142 145 3 850.01 1.59 442 142 146 4 844.34 3.08 443 143 146 3 841.49 1.65 444 143 147 4 841.48 1.65 445 143 164 21 792.02 0.85 446 143 184 41 448.60 27.66 447 143 188 45 448.38 27.64 448 144 147 3 851.85 5.35 449 144 148 4 832.34 2.93 450 144 187 43 448.54 27.64 451 144 188 44 448.44 27.61 452 144 191 47 448.22 27.59 453 145 148 3 864.02 0.74 454 145 149 4 846.01 4.85 455 146 149 3 857.79 1.36 456 146 150 4 846.01 4.85 457 147 150 3 851.91 1.56 458 147 151 4 831.83 2.23 459 147 162 15 792.04 0.84 460 147 188 41 448.25 27.60 461 147 191 44 447.89 27.70 462 148 151 3 846.38 4.45 463 148 152 4 831.78 2.21 464 148 191 43 447.89 27.70 465 149 152 3 867.97 1.03 466 149 153 4 850.14 6.93 467 150 153 3 874.69 2.06 468 150 154 4 845.96 9.02 469 150 162 12 792.01 0.83 470 151 154 3 854.97 4.51 471 151 157 6 794.39 1.58 472 151 191 40 447.86 27.70 473 154 157 3 878.68 1.79 474 154 160 6 793.30 1.00 475 156 160 4 865.00 0.47 476 157 160 3 860.69 2.62 477 157 162 5 858.38 3.78 478 157 192 35 446.75 27.70 479 157 196 39 437.74 30.36 480 157 200 43 437.38 30.39 481 157 204 47 437.09 30.43 482 160 204 44 437.21 30.43 483 161 205 44 437.06 30.43 484 161 207 46 436.56 30.59 485 162 192 30 446.87 27.68 486 162 205 43 437.20 30.43 487 162 206 44 436.80 30.55 488 162 207 45 436.76 30.53 489 163 207 44 436.80 30.55 490 163 230 67 121.59 0.50 491 164 176 12 448.79 27.64 492 164 184 20 448.39 27.64 493 164 185 21 448.37 27.64 494 164 188 24 448.36 27.64 495 164 207 43 437.10 30.43 496 164 208 44 436.72 30.56 497 164 209 45 436.51 30.57 498 165 170 5 698.21 61.59 499 165 209 44 436.38 30.52 500 165 230 65 121.81 0.56 501 166 169 3 862.98 6.32 502 166 170 4 841.91 0.73 503 166 176 10 449.32 27.74 504 166 208 42 437.14 30.43 505 166 209 43 436.78 30.56 506 167 170 3 861.31 10.11 507 168 172 4 839.04 0.70 508 168 174 6 467.49 24.14 509 168 176 8 451.49 27.04 510 169 172 3 842.45 0.36 511 169 174 5 449.79 27.88 512 169 175 6 449.42 27.77 513 169 208 39 437.24 30.46 514 169 210 41 436.27 30.44 515 169 211 42 436.31 30.48 516 170 210 40 436.27 30.44 517 171 211 40 436.62 30.57 518 172 211 39 436.76 30.56 519 173 211 38 436.76 30.56 520 174 211 37 436.81 30.56 521 175 208 33 437.55 30.39 522 175 210 35 436.81 30.55 523 175 211 36 436.80 30.55 524 175 216 41 388.22 28.69 525 176 180 4 815.05 1.53 526 176 181 5 754.69 28.24 527 176 208 32 437.58 30.38 528 176 220 44 223.81 1.70 529 177 180 3 835.47 1.36 530 177 181 4 828.90 1.93 531 177 220 43 223.82 1.70 532 178 181 3 853.86 0.51 533 178 182 4 828.90 1.93 534 178 219 41 122.78 0.55 535 178 220 42 122.71 0.52 536 178 223 45 122.66 0.50 537 179 182 3 851.77 0.54 538 179 183 4 832.84 0.04 539 180 183 3 842.91 5.07 540 180 184 4 832.85 0.04 541 181 184 3 854.62 0.08 542 181 185 4 843.80 5.56 543 181 208 27 437.62 30.37 544 181 220 39 122.72 0.51 545 181 226 45 122.42 0.56 546 182 185 3 853.74 0.59 547 182 186 4 843.77 5.57 548 182 223 41 122.43 0.57 549 182 224 42 122.43 0.57 550 182 225 43 122.42 0.56 551 182 226 44 122.39 0.57 552 183 186 3 858.15 1.61 553 183 187 4 853.72 0.59 554 184 187 3 853.74 0.59 555 184 188 4 853.72 0.59 556 185 188 3 853.60 0.54 557 185 189 4 828.94 1.81 558 185 206 21 437.69 30.38 559 185 207 22 437.68 30.38 560 185 208 23 437.62 30.37 561 185 225 40 122.19 0.50 562 185 226 41 122.14 0.49 563 186 189 3 848.62 3.04 564 186 190 4 828.23 1.47 565 186 225 39 122.14 0.49 566 187 190 3 842.91 5.88 567 187 191 4 828.21 1.47 568 188 191 3 835.48 2.31 569 188 192 4 835.49 2.31 570 188 206 18 437.68 30.37 571 189 192 3 844.05 1.01 572 189 193 4 835.48 2.31 573 189 201 12 437.98 30.32 574 189 204 15 437.86 30.35 575 189 206 17 437.69 30.37 576 189 225 36 122.14 0.49 577 189 227 38 122.11 0.48 578 190 193 3 848.40 2.28 579 190 194 4 837.36 3.25 580 191 194 3 848.41 2.27 581 191 195 4 833.38 5.24 582 191 196 5 823.15 0.12 583 192 195 3 855.78 5.96 584 192 196 4 823.02 0.09 585 192 197 5 821.95 0.15 586 192 200 8 471.44 23.86 587 192 201 9 437.99 30.33 588 192 204 12 437.85 30.35 589 192 206 14 437.69 30.37 590 193 197 4 681.21 3.73 591 193 198 5 467.45 26.75 592 193 201 8 441.38 31.88 593 196 200 4 822.87 2.23 594 197 200 3 834.04 1.66 595 197 201 4 830.24 3.56 596 198 201 3 849.05 5.83 597 198 202 4 825.24 1.05 598 199 202 3 850.35 5.19 599 199 203 4 825.23 1.05 600 200 203 3 834.88 1.25 601 200 204 4 799.86 1.48 602 204 227 23 122.06 0.48 603 205 227 22 121.78 0.57 604 206 226 20 122.14 0.49 605 206 227 21 122.10 0.48 606 207 227 20 122.12 0.48 607 207 228 21 121.77 0.56 608 207 229 22 121.73 0.54 609 207 230 23 121.73 0.54 610 208 212 4 852.98 0.41 611 208 220 12 122.50 0.41 612 208 226 18 122.30 0.51 613 208 228 20 122.14 0.48 614 208 229 21 121.81 0.57 615 208 230 22 121.75 0.53 616 209 230 21 121.75 0.53 617 211 216 5 809.59 1.76 618 212 216 4 808.19 1.06 619 212 217 5 495.45 25.19 620 212 220 8 463.88 24.20 621 212 229 17 122.48 0.53 622 212 231 19 122.14 0.48 623 212 234 22 121.15 0.50 624 212 243 31 51.50 0.09 625 213 216 3 862.36 4.72 626 213 217 4 634.91 32.60 627 213 234 21 121.15 0.70 628 213 239 26 54.31 0.90 629 213 243 30 51.61 0.12 630 214 217 3 851.54 0.69 631 214 239 25 54.24 0.94 632 214 242 28 52.05 0.09 633 214 243 29 51.54 0.12 634 216 219 3 873.71 2.54 635 216 220 4 830.56 1.31 636 217 220 3 857.28 4.97 637 217 221 4 830.56 1.30 638 217 229 12 122.53 0.55 639 217 242 25 51.63 0.10 640 217 243 26 48.94 0.64 641 217 246 29 38.30 0.63 642 218 221 3 854.10 0.59 643 218 222 4 838.20 2.51 644 218 246 28 38.29 0.63 645 218 248 30 38.20 0.58 646 219 222 3 855.03 0.80 647 219 223 4 854.36 0.46 648 220 223 3 858.34 0.32 649 220 226 6 282.69 79.49 650 220 229 9 122.50 0.57 651 221 226 5 282.69 79.49 652 221 229 8 122.50 0.57 653 221 246 25 38.19 0.59 654 221 247 26 37.15 0.93 655 223 226 3 841.44 8.31 656 226 229 3 997.71 0.22 657 229 243 14 47.66 0.14 658 229 246 17 38.18 0.58 659 231 234 3 883.72 2.94 660 231 235 4 809.07 5.44 661 231 240 9 54.30 0.90 662 231 243 12 48.41 0.38 663 232 235 3 866.75 4.14 664 233 236 3 875.18 0.06 665 233 237 4 860.20 6.63 666 234 237 3 836.78 1.43 667 234 239 5 465.49 29.79 668 234 240 6 98.41 17.96 669 234 243 9 321.69 93.69 670 235 240 5 315.24 94.59 671 237 240 3 869.60 1.90 672 238 241 3 869.60 1.91 673 239 242 3 843.70 6.55 674 239 243 4 829.01 1.45 675 240 243 3 843.98 6.03 676 240 244 4 829.01 1.45 677 241 244 3 829.06 1.48 678 241 245 4 821.88 2.10 679 242 245 3 816.69 2.44 680 242 246 4 807.98 4.84 681 243 246 3 829.05 6.34 682 245 248 3 830.76 0.33