BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 177.97 1.98 2 4 204 200 177.94 1.97 3 4 205 201 166.03 0.36 4 4 224 220 121.14 1.00 5 4 225 221 121.12 0.99 6 4 227 223 121.00 1.02 7 5 36 31 419.68 60.61 8 5 38 33 419.76 60.67 9 5 205 200 177.94 1.97 10 6 37 31 419.75 60.65 11 6 38 32 419.82 60.65 12 6 221 215 121.08 1.02 13 6 227 221 121.05 1.02 14 6 228 222 121.01 1.02 15 6 229 223 121.02 1.02 16 6 243 237 44.26 0.21 17 6 246 240 33.48 1.03 18 6 247 241 28.43 1.21 19 7 38 31 419.85 60.66 20 7 40 33 419.86 60.65 21 7 90 83 195.65 0.24 22 7 124 117 185.47 0.07 23 7 161 154 185.37 0.10 24 7 205 198 185.22 0.15 25 7 207 200 173.59 0.20 26 7 227 220 121.15 1.00 27 7 228 221 121.05 1.02 28 7 229 222 121.02 1.02 29 8 37 29 419.91 60.66 30 8 38 30 419.91 60.65 31 8 39 31 419.87 60.66 32 8 40 32 419.87 60.66 33 8 229 221 121.02 1.02 34 8 231 223 121.00 1.02 35 8 240 232 57.61 0.15 36 8 243 235 44.21 0.20 37 8 244 236 36.99 0.48 38 9 40 31 419.87 60.66 39 9 229 220 121.14 1.01 40 9 230 221 121.02 1.02 41 9 231 222 121.01 1.02 42 10 40 30 419.91 60.65 43 10 41 31 419.87 60.65 44 10 42 32 419.89 60.63 45 10 231 221 121.02 1.02 46 10 235 225 76.38 0.06 47 10 240 230 57.68 0.13 48 11 42 31 419.89 60.63 49 11 64 53 297.73 0.33 50 11 92 81 195.58 0.27 51 11 95 84 195.14 0.30 52 11 230 219 121.47 0.93 53 11 231 220 121.12 0.99 54 11 232 221 121.02 1.02 55 11 235 224 85.95 1.36 56 12 15 3 859.51 0.76 57 12 21 9 459.19 41.51 58 12 24 12 421.36 60.41 59 12 25 13 421.36 60.41 60 12 41 29 420.26 60.50 61 12 42 30 420.20 60.50 62 12 43 31 420.25 60.47 63 12 44 32 420.39 60.40 64 12 51 39 385.43 42.88 65 12 55 43 300.12 0.06 66 12 64 52 297.75 0.32 67 12 235 223 112.37 1.26 68 12 236 224 81.66 0.03 69 13 44 31 420.40 60.40 70 13 64 51 297.75 0.32 71 13 95 82 195.53 0.26 72 13 97 84 195.49 0.28 73 13 170 157 195.12 0.14 74 13 232 219 121.47 0.93 75 15 21 6 556.20 0.88 76 15 44 29 420.48 60.38 77 15 236 221 120.98 1.01 78 16 20 4 708.35 59.97 79 16 21 5 555.98 0.77 80 16 47 31 421.44 59.88 81 17 20 3 838.59 4.22 82 17 21 4 742.97 30.55 83 17 47 30 421.47 59.87 84 18 22 4 822.42 5.25 85 18 47 29 421.47 59.87 86 18 50 32 415.40 56.83 87 19 22 3 849.34 5.33 88 19 23 4 845.08 3.21 89 20 23 3 849.34 5.34 90 20 24 4 833.22 9.14 91 21 24 3 849.34 5.34 92 21 25 4 823.91 4.50 93 21 44 23 421.72 59.81 94 21 47 26 421.66 59.76 95 21 50 29 403.26 49.83 96 22 25 3 850.39 0.55 97 22 26 4 842.85 2.17 98 22 50 28 403.25 49.83 99 23 26 3 849.24 1.02 100 23 27 4 842.90 2.15 101 24 27 3 860.53 3.71 102 24 28 4 845.00 3.20 103 24 235 211 118.38 2.08 104 24 236 212 82.44 0.01 105 25 28 3 856.21 5.87 106 25 29 4 845.01 3.21 107 25 50 25 404.23 49.33 108 25 51 26 400.64 48.47 109 25 54 29 302.82 1.40 110 25 55 30 302.75 1.44 111 26 29 3 862.34 2.81 112 26 30 4 845.21 3.31 113 27 30 3 864.02 3.65 114 27 31 4 839.59 6.12 115 28 31 3 849.38 5.63 116 28 33 5 830.82 10.30 117 28 41 13 423.89 58.77 118 28 55 27 303.21 1.67 119 28 59 31 302.30 2.12 120 28 240 212 57.70 0.14 121 28 241 213 57.66 0.15 122 28 244 216 57.38 0.09 123 29 54 25 303.29 1.64 124 29 55 26 303.25 1.65 125 29 56 27 303.19 1.69 126 29 59 30 302.32 2.12 127 31 241 210 57.65 0.15 128 32 241 209 57.63 0.14 129 32 245 213 46.51 0.47 130 33 241 208 57.58 0.17 131 33 244 211 46.45 0.49 132 33 245 212 46.05 0.62 133 34 37 3 858.82 0.36 134 34 245 211 43.08 0.46 135 34 248 214 29.98 0.67 136 36 248 212 29.41 0.86 137 37 244 207 39.73 0.62 138 38 90 52 195.77 0.33 139 38 205 167 185.50 0.10 140 39 59 20 335.56 19.13 141 39 60 21 335.56 19.14 142 39 240 201 57.62 0.15 143 39 244 205 37.61 0.18 144 40 59 19 335.62 19.11 145 40 60 20 335.56 19.14 146 40 61 21 335.55 19.14 147 40 62 22 335.43 19.09 148 40 90 50 195.66 0.24 149 40 124 84 190.88 0.00 150 41 55 14 336.20 18.89 151 41 59 18 335.67 19.09 152 41 60 19 335.65 19.09 153 41 61 20 335.44 19.09 154 41 62 21 335.44 19.09 155 41 240 199 57.69 0.13 156 42 62 20 335.44 19.08 157 42 64 22 335.38 19.09 158 42 92 50 195.99 0.43 159 43 48 5 458.84 42.38 160 43 51 8 415.06 20.50 161 43 62 19 335.63 19.09 162 43 64 21 335.44 19.09 163 44 47 3 846.42 2.94 164 44 48 4 822.59 4.91 165 44 64 20 335.55 19.11 166 45 48 3 835.26 1.36 167 45 78 33 212.25 0.24 168 47 50 3 848.68 1.79 169 47 51 4 844.02 0.53 170 48 51 3 856.46 2.10 171 48 52 4 844.02 0.53 172 48 61 13 336.03 19.07 173 48 62 14 336.00 19.07 174 48 63 15 335.80 19.07 175 48 78 30 212.25 0.24 176 48 83 35 196.63 0.68 177 48 86 38 194.15 0.32 178 49 52 3 848.73 1.81 179 49 53 4 844.02 0.53 180 49 86 37 188.46 0.03 181 50 53 3 847.99 1.46 182 50 54 4 840.81 1.07 183 51 54 3 861.13 0.91 184 51 55 4 839.65 1.63 185 51 61 10 336.07 19.05 186 52 55 3 847.65 1.34 187 52 56 4 848.43 1.74 188 52 57 5 842.18 2.90 189 52 59 7 544.56 1.26 190 52 60 8 336.31 19.12 191 52 61 9 336.09 19.05 192 52 86 34 195.59 0.17 193 52 87 35 194.13 0.33 194 52 88 36 191.26 2.35 195 55 59 4 834.28 0.43 196 55 61 6 459.81 41.59 197 56 59 3 869.04 3.82 198 61 88 27 196.12 0.41 199 61 89 28 196.09 0.42 200 61 90 29 195.76 0.29 201 62 90 28 195.76 0.28 202 62 91 29 195.81 0.30 203 62 92 30 196.03 0.42 204 63 78 15 212.16 0.29 205 63 83 20 196.51 0.38 206 63 90 27 196.20 0.42 207 63 91 28 196.15 0.45 208 63 92 29 196.10 0.42 209 64 92 28 196.13 0.42 210 65 78 13 212.25 0.26 211 65 91 26 196.50 0.41 212 65 92 27 196.32 0.43 213 65 93 28 196.23 0.47 214 65 98 33 196.36 0.52 215 65 104 39 201.62 1.03 216 65 112 47 203.31 0.73 217 66 78 12 212.25 0.25 218 66 79 13 212.19 0.28 219 66 80 14 212.19 0.28 220 66 83 17 212.09 0.24 221 66 91 25 196.71 0.46 222 66 93 27 196.69 0.46 223 66 112 46 203.39 0.77 224 67 74 7 212.50 0.23 225 67 77 10 212.36 0.22 226 67 78 11 212.34 0.22 227 67 80 13 212.25 0.26 228 67 108 41 203.52 0.83 229 67 112 45 203.51 0.83 230 68 108 40 203.91 0.64 231 68 111 43 203.99 0.97 232 68 112 44 203.53 0.83 233 68 115 47 206.03 0.83 234 69 74 5 819.09 7.63 235 69 79 10 212.36 0.21 236 69 115 46 206.11 0.86 237 70 79 9 212.36 0.21 238 70 81 11 212.34 0.21 239 70 115 45 206.12 0.86 240 74 77 3 850.53 0.04 241 78 82 4 842.87 1.78 242 78 83 5 840.95 0.78 243 79 82 3 863.98 5.12 244 79 83 4 845.71 0.18 245 80 83 3 857.20 5.56 246 80 84 4 842.76 1.68 247 80 115 35 462.05 29.75 248 80 116 36 412.42 54.55 249 80 119 39 556.54 2.27 250 80 121 41 480.94 25.98 251 81 84 3 858.75 4.78 252 81 85 4 842.75 1.68 253 82 85 3 850.92 2.40 254 82 86 4 842.78 1.69 255 83 86 3 868.15 0.20 256 83 87 4 842.77 1.70 257 83 88 5 712.51 60.90 258 83 90 7 578.85 4.38 259 83 121 38 580.51 5.11 260 84 87 3 853.63 0.11 261 84 88 4 836.76 1.18 262 84 119 35 600.88 0.62 263 84 121 37 586.14 7.98 264 88 121 33 662.90 0.57 265 89 121 32 662.89 0.57 266 89 122 33 662.87 0.58 267 90 121 31 662.90 0.57 268 90 122 32 662.89 0.57 269 90 123 33 662.87 0.58 270 90 124 34 662.86 0.58 271 90 159 69 662.87 0.58 272 90 161 71 662.86 0.58 273 91 116 25 663.02 0.58 274 91 121 30 663.01 0.58 275 91 122 31 663.00 0.58 276 91 123 32 662.87 0.58 277 91 124 33 662.87 0.58 278 92 95 3 901.08 0.61 279 92 124 32 662.87 0.58 280 92 126 34 662.83 0.57 281 92 230 138 121.61 0.88 282 93 112 19 663.12 0.54 283 93 113 20 663.06 0.56 284 93 116 23 663.18 0.57 285 93 123 30 663.00 0.58 286 93 124 31 663.00 0.58 287 93 125 32 662.86 0.57 288 93 126 33 662.86 0.57 289 94 99 5 671.77 4.15 290 94 109 15 663.09 0.54 291 94 126 32 662.85 0.57 292 95 98 3 851.53 1.01 293 95 99 4 843.07 1.79 294 95 126 31 662.90 0.58 295 95 165 70 662.85 0.57 296 95 230 135 121.83 0.83 297 96 99 3 853.49 1.98 298 96 100 4 839.65 3.50 299 96 167 71 643.10 3.73 300 96 170 74 582.62 0.10 301 97 100 3 860.62 1.59 302 97 101 4 692.18 6.03 303 97 102 5 671.78 4.17 304 97 170 73 621.84 0.55 305 98 102 4 671.61 4.25 306 98 103 5 671.61 4.25 307 98 104 6 663.74 0.52 308 99 103 4 663.78 0.53 309 99 104 5 663.75 0.53 310 99 106 7 663.74 0.53 311 99 109 10 663.74 0.53 312 99 126 27 663.02 0.58 313 99 127 28 663.01 0.58 314 99 146 47 663.19 0.57 315 99 150 51 663.39 0.53 316 100 130 30 662.88 0.57 317 100 167 67 661.18 0.82 318 104 108 4 847.05 4.66 319 104 109 5 833.67 1.97 320 104 112 8 819.24 2.93 321 105 108 3 851.91 4.56 322 105 109 4 838.81 1.99 323 106 109 3 862.10 0.83 324 106 110 4 843.72 2.99 325 106 146 40 663.33 0.52 326 106 149 43 663.37 0.53 327 107 110 3 846.24 1.73 328 107 111 4 843.74 2.98 329 108 111 3 850.77 6.50 330 108 112 4 836.33 0.73 331 109 112 3 841.51 1.83 332 109 113 4 840.14 1.10 333 109 150 41 663.46 0.50 334 110 113 3 862.69 2.04 335 110 114 4 845.70 3.81 336 110 149 39 663.47 0.50 337 110 153 43 663.90 0.68 338 111 114 3 860.05 3.35 339 111 115 4 857.50 2.09 340 112 115 3 860.05 3.35 341 112 116 4 852.00 4.84 342 113 116 3 861.40 4.03 343 113 117 4 856.97 2.35 344 113 123 10 663.84 0.64 345 113 125 12 663.43 0.52 346 113 150 37 663.70 0.58 347 113 153 40 664.43 0.48 348 113 154 41 664.43 0.48 349 114 117 3 864.41 5.54 350 114 118 4 847.00 2.62 351 114 153 39 664.07 0.66 352 115 118 3 849.02 1.61 353 115 119 4 847.00 2.62 354 116 119 3 865.02 1.66 355 116 120 4 847.00 2.62 356 116 121 5 795.33 1.78 357 116 123 7 744.92 26.96 358 117 120 3 839.59 0.72 359 117 121 4 813.61 6.67 360 117 122 5 798.67 0.79 361 117 123 6 745.09 26.89 362 117 154 37 691.66 0.12 363 117 156 39 723.34 3.72 364 117 160 43 691.64 0.11 365 122 159 37 785.43 0.43 366 123 159 36 785.48 0.43 367 123 160 37 785.45 0.43 368 123 161 38 785.44 0.43 369 124 161 37 785.45 0.42 370 124 163 39 785.43 0.43 371 124 207 83 434.45 30.34 372 124 230 106 121.36 0.85 373 125 150 25 785.74 0.42 374 125 161 36 785.51 0.43 375 125 162 37 785.47 0.42 376 125 163 38 785.46 0.42 377 126 163 37 785.47 0.42 378 126 230 104 121.64 0.88 379 127 143 16 786.12 0.45 380 127 146 19 786.08 0.47 381 127 147 20 786.00 0.48 382 127 150 23 786.00 0.48 383 127 162 35 785.57 0.42 384 127 163 36 785.54 0.42 385 127 164 37 785.47 0.42 386 127 165 38 785.47 0.42 387 127 188 61 442.44 31.16 388 128 143 15 786.19 0.43 389 128 146 18 786.17 0.43 390 128 164 36 785.51 0.42 391 128 165 37 785.49 0.42 392 128 166 38 785.44 0.43 393 128 167 39 785.42 0.43 394 129 133 4 834.47 3.98 395 129 134 5 820.53 2.94 396 129 139 10 786.27 0.46 397 129 142 13 786.24 0.46 398 129 143 14 786.24 0.45 399 129 164 35 785.57 0.42 400 129 166 37 785.49 0.42 401 129 167 38 785.47 0.42 402 129 168 39 785.46 0.42 403 130 133 3 849.02 5.49 404 130 134 4 847.48 6.07 405 130 167 37 785.48 0.42 406 130 168 38 785.47 0.42 407 131 134 3 847.49 6.07 408 131 135 4 838.90 1.77 409 131 168 37 785.49 0.42 410 132 135 3 857.05 1.28 411 132 136 4 847.49 6.07 412 133 136 3 857.05 1.28 413 133 137 4 844.91 7.36 414 133 138 5 787.69 0.05 415 133 142 9 786.48 0.49 416 134 137 3 843.40 2.66 417 134 139 5 786.47 0.50 418 134 164 30 785.70 0.41 419 134 168 34 785.53 0.42 420 134 176 42 511.07 26.48 421 134 180 46 444.41 30.80 422 134 184 50 443.67 31.14 423 138 141 3 852.10 2.52 424 138 142 4 842.61 3.94 425 139 142 3 871.54 2.74 426 139 143 4 848.79 0.87 427 139 164 25 785.66 0.42 428 139 184 45 442.98 31.21 429 140 143 3 875.15 0.94 430 140 144 4 850.21 0.17 431 140 187 47 442.51 31.16 432 141 144 3 863.11 0.78 433 141 145 4 850.20 0.15 434 142 145 3 852.72 1.41 435 142 146 4 843.50 3.19 436 143 146 3 850.16 0.14 437 143 147 4 838.68 5.59 438 143 164 21 785.57 0.42 439 143 184 41 442.50 31.17 440 143 188 45 442.47 31.16 441 144 147 3 852.75 1.45 442 144 148 4 838.68 5.59 443 144 187 43 442.47 31.16 444 144 188 44 442.47 31.16 445 144 191 47 442.23 31.07 446 145 148 3 858.94 4.54 447 145 149 4 852.74 1.45 448 146 149 3 860.02 2.18 449 146 150 4 852.73 1.46 450 147 150 3 851.09 6.44 451 147 151 4 820.34 1.40 452 147 162 15 785.61 0.42 453 147 188 41 442.39 31.14 454 147 191 44 442.14 31.07 455 148 151 3 842.59 6.54 456 148 152 4 817.71 0.96 457 148 191 43 441.93 30.96 458 149 153 4 858.07 1.28 459 150 153 3 865.25 0.63 460 150 154 4 846.55 4.48 461 150 162 12 785.57 0.42 462 151 154 3 851.20 4.72 463 151 157 6 786.74 0.30 464 151 191 40 441.90 30.95 465 151 196 45 435.60 30.29 466 154 157 3 881.03 5.73 467 154 160 6 785.81 0.40 468 157 160 3 837.65 3.10 469 157 162 5 841.87 1.84 470 157 192 35 440.35 30.61 471 157 196 39 435.63 30.28 472 157 200 43 434.89 30.30 473 157 204 47 434.62 30.33 474 161 205 44 434.56 30.30 475 161 207 46 434.45 30.34 476 162 192 30 440.52 30.70 477 162 205 43 434.72 30.30 478 162 206 44 434.56 30.30 479 162 207 45 434.46 30.34 480 163 207 44 434.53 30.28 481 163 230 67 121.58 0.86 482 164 176 12 443.69 31.16 483 164 184 20 442.46 31.18 484 164 185 21 442.44 31.16 485 164 188 24 442.43 31.16 486 164 207 43 434.68 30.30 487 164 208 44 434.45 30.25 488 164 209 45 434.29 30.30 489 165 170 5 810.53 4.69 490 165 209 44 434.25 30.28 491 165 210 45 434.20 30.29 492 165 230 65 121.89 0.83 493 166 169 3 842.41 3.21 494 166 170 4 847.50 2.80 495 166 176 10 443.93 31.05 496 166 208 42 434.67 30.30 497 166 209 43 434.60 30.28 498 167 170 3 858.66 2.78 499 168 172 4 840.69 0.99 500 168 174 6 540.42 4.60 501 168 176 8 456.78 24.75 502 169 172 3 852.75 0.27 503 169 174 5 495.20 17.95 504 169 175 6 456.69 24.77 505 169 210 41 434.27 30.22 506 169 211 42 434.26 30.22 507 170 210 40 434.18 30.21 508 172 211 39 434.33 30.19 509 174 211 37 434.53 30.23 510 175 208 33 434.89 30.36 511 175 210 35 434.66 30.30 512 175 211 36 434.65 30.30 513 175 216 41 402.73 31.03 514 176 180 4 804.20 4.07 515 176 181 5 756.09 28.14 516 176 208 32 435.04 30.30 517 177 180 3 857.74 9.59 518 177 181 4 834.25 0.65 519 178 181 3 862.79 7.06 520 178 182 4 842.18 4.58 521 178 219 41 234.02 1.53 522 178 220 42 231.49 2.79 523 178 223 45 174.72 25.46 524 179 182 3 865.95 5.49 525 179 183 4 845.22 4.86 526 180 183 3 856.32 0.67 527 180 184 4 853.50 0.72 528 181 184 3 858.92 1.96 529 181 185 4 853.18 0.89 530 181 208 27 435.10 30.27 531 181 220 39 221.08 2.40 532 181 226 45 122.14 0.76 533 182 185 3 854.12 0.42 534 182 186 4 850.32 2.27 535 182 223 41 122.19 0.77 536 182 224 42 122.19 0.77 537 182 225 43 122.12 0.76 538 183 186 3 858.77 1.89 539 183 187 4 847.98 3.52 540 184 187 3 843.13 1.32 541 184 188 4 839.47 0.74 542 185 188 3 855.34 0.17 543 185 189 4 834.22 3.37 544 185 206 21 435.18 30.28 545 185 207 22 435.18 30.28 546 185 208 23 435.07 30.29 547 185 225 40 122.10 0.77 548 185 226 41 122.06 0.77 549 186 189 3 855.47 0.40 550 186 190 4 834.21 3.37 551 186 225 39 122.07 0.77 552 187 190 3 841.14 5.12 553 187 191 4 834.20 3.37 554 188 191 3 860.30 2.96 555 188 192 4 835.89 5.33 556 188 206 18 435.19 30.27 557 189 192 3 853.16 6.53 558 189 193 4 835.00 5.78 559 189 201 12 435.51 30.23 560 189 204 15 435.41 30.21 561 189 206 17 435.18 30.27 562 189 225 36 122.07 0.77 563 189 227 38 122.03 0.76 564 190 193 3 844.74 0.91 565 190 194 4 834.94 5.80 566 191 194 3 849.78 0.84 567 191 195 4 834.37 6.08 568 191 196 5 826.29 2.04 569 192 195 3 844.73 0.91 570 192 196 4 826.26 2.06 571 192 197 5 815.44 3.18 572 192 200 8 451.16 27.48 573 192 201 9 435.69 30.26 574 192 204 12 435.43 30.26 575 192 206 14 435.24 30.24 576 193 197 4 746.32 25.84 577 193 198 5 460.19 23.20 578 193 201 8 435.80 30.30 579 196 200 4 823.06 0.64 580 197 200 3 853.88 5.49 581 197 201 4 824.76 1.50 582 198 201 3 830.10 4.10 583 198 202 4 824.76 1.50 584 199 202 3 840.77 6.50 585 199 203 4 823.05 0.64 586 200 203 3 838.12 5.18 587 200 204 4 818.48 2.89 588 205 227 22 121.63 0.84 589 206 226 20 122.08 0.76 590 206 227 21 121.77 0.81 591 207 227 20 122.08 0.77 592 207 228 21 121.64 0.84 593 207 229 22 121.64 0.85 594 207 230 23 121.62 0.84 595 208 212 4 864.76 0.60 596 208 220 12 122.28 0.78 597 208 228 20 122.08 0.77 598 208 229 21 121.70 0.85 599 208 230 22 121.67 0.86 600 209 212 3 882.55 4.03 601 209 230 21 121.68 0.86 602 211 216 5 825.34 2.23 603 212 216 4 825.34 2.23 604 212 217 5 530.28 25.89 605 212 220 8 477.42 32.42 606 212 229 17 122.19 0.77 607 212 231 19 122.07 0.78 608 212 234 22 109.01 5.32 609 212 243 31 51.02 0.10 610 213 216 3 864.12 5.19 611 213 217 4 825.42 2.19 612 213 234 21 106.70 6.48 613 213 239 26 59.30 0.65 614 213 243 30 51.79 0.48 615 214 217 3 852.94 2.29 616 214 239 25 59.28 0.66 617 214 242 28 56.48 0.26 618 214 243 29 51.78 0.48 619 216 219 3 868.67 3.04 620 216 220 4 830.83 4.02 621 217 220 3 846.73 3.94 622 217 221 4 830.83 4.02 623 217 229 12 122.20 0.76 624 217 243 26 44.38 0.20 625 217 246 29 33.94 1.18 626 218 221 3 848.20 4.67 627 218 222 4 840.87 1.00 628 218 246 28 33.90 1.16 629 219 222 3 857.08 0.23 630 219 223 4 844.78 2.96 631 220 223 3 878.06 6.29 632 220 226 6 318.19 97.13 633 220 229 9 122.12 0.76 634 221 226 5 122.43 0.66 635 221 229 8 122.31 0.67 636 221 246 25 33.72 1.14 637 221 247 26 33.38 1.20 638 223 226 3 850.88 2.85 639 229 243 14 44.22 0.20 640 229 246 17 33.80 1.11 641 231 234 3 873.40 0.45 642 231 235 4 820.94 2.17 643 231 240 9 57.69 0.13 644 231 243 12 46.18 0.01 645 232 235 3 856.32 0.85 646 233 236 3 866.63 2.85 647 233 237 4 854.52 0.04 648 234 237 3 846.93 3.75 649 234 239 5 451.68 24.48 650 234 240 6 224.59 83.57 651 234 243 9 57.45 0.07 652 235 240 5 313.83 45.81 653 237 240 3 868.64 2.38 654 238 241 3 872.85 0.31 655 239 242 3 856.55 0.91 656 239 243 4 849.06 4.65 657 240 243 3 857.15 0.60 658 240 244 4 849.06 4.65 659 241 244 3 843.05 1.91 660 241 245 4 833.96 2.64 661 242 245 3 826.88 0.91 662 242 246 4 826.86 0.90 663 243 246 3 826.69 0.98 664 245 248 3 831.87 1.88