BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 174.65 0.42 2 4 204 200 168.15 0.12 3 4 205 201 166.52 0.36 4 4 224 220 116.04 0.96 5 4 227 223 115.35 1.02 6 5 35 30 547.19 0.91 7 5 36 31 547.16 0.91 8 5 37 32 547.14 0.91 9 5 38 33 547.11 0.90 10 5 205 200 168.12 0.12 11 6 36 30 547.19 0.91 12 6 37 31 547.14 0.91 13 6 38 32 547.14 0.91 14 6 221 215 115.95 0.91 15 6 227 221 115.95 0.90 16 6 228 222 115.88 0.88 17 6 229 223 115.93 0.90 18 6 246 240 34.45 1.21 19 6 247 241 28.79 0.29 20 7 38 31 547.15 0.90 21 7 40 33 547.14 0.90 22 7 90 83 194.08 0.09 23 7 124 117 183.02 0.91 24 7 161 154 183.00 0.91 25 7 205 198 182.78 0.96 26 7 207 200 167.98 0.09 27 7 227 220 116.11 0.93 28 7 228 221 115.95 0.90 29 7 229 222 115.94 0.90 30 8 37 29 547.21 0.91 31 8 38 30 547.18 0.91 32 8 39 31 547.14 0.90 33 8 40 32 547.14 0.90 34 8 229 221 115.95 0.90 35 8 231 223 115.87 0.88 36 8 240 232 51.81 1.18 37 8 243 235 40.26 0.08 38 8 244 236 38.26 0.15 39 9 40 31 547.14 0.90 40 9 229 220 116.12 0.93 41 9 230 221 115.95 0.90 42 9 231 222 115.92 0.91 43 10 40 30 547.18 0.91 44 10 41 31 547.14 0.90 45 10 42 32 547.13 0.91 46 10 231 221 115.94 0.90 47 10 235 225 76.98 0.42 48 10 240 230 51.85 1.17 49 11 64 53 295.22 1.93 50 11 92 81 195.09 0.10 51 11 95 84 193.24 0.36 52 11 230 219 117.87 0.67 53 11 231 220 116.09 0.91 54 11 232 221 115.92 0.91 55 11 235 224 85.92 0.86 56 12 15 3 860.47 0.91 57 12 21 9 548.50 1.10 58 12 24 12 548.48 1.09 59 12 25 13 548.40 1.06 60 12 41 29 547.22 0.90 61 12 42 30 547.16 0.90 62 12 43 31 547.16 0.90 63 12 44 32 547.15 0.91 64 12 64 52 295.25 1.94 65 12 235 223 108.56 1.36 66 12 236 224 82.88 0.64 67 13 44 31 547.16 0.91 68 13 64 51 295.25 1.94 69 13 95 82 195.10 0.09 70 13 97 84 193.75 0.59 71 13 170 157 193.83 0.56 72 13 232 219 117.86 0.68 73 15 44 29 547.22 0.90 74 15 236 221 115.91 0.90 75 16 20 4 833.96 2.10 76 16 21 5 743.44 34.29 77 16 47 31 546.90 0.82 78 17 20 3 860.41 5.62 79 17 21 4 836.31 0.33 80 17 47 30 546.78 0.77 81 18 21 3 862.52 0.39 82 18 22 4 836.31 0.33 83 18 47 29 546.74 0.79 84 18 50 32 532.12 1.53 85 19 22 3 859.92 0.91 86 19 23 4 836.36 0.30 87 20 23 3 847.45 2.03 88 20 24 4 836.35 0.31 89 21 24 3 847.31 1.95 90 21 25 4 832.63 1.54 91 21 44 23 547.21 0.90 92 21 47 26 546.58 0.85 93 21 50 29 514.28 4.01 94 21 236 215 108.37 1.53 95 22 25 3 847.56 4.32 96 22 26 4 850.83 2.68 97 22 50 28 499.38 2.70 98 23 26 3 850.84 2.68 99 23 27 4 850.85 2.67 100 24 27 3 850.85 2.67 101 24 28 4 850.85 2.67 102 24 235 211 113.56 1.06 103 24 236 212 94.17 1.73 104 25 28 3 861.51 3.60 105 25 29 4 838.12 3.70 106 25 50 25 498.47 2.27 107 25 51 26 487.01 4.11 108 25 54 29 297.68 1.66 109 25 55 30 297.42 1.75 110 26 29 3 847.26 0.88 111 26 30 4 842.41 1.54 112 27 30 3 865.33 1.35 113 27 31 4 847.23 0.90 114 27 238 211 72.85 0.65 115 28 31 3 850.15 0.57 116 28 41 13 547.23 0.91 117 28 55 27 297.38 1.77 118 28 59 31 296.24 1.86 119 28 240 212 56.41 1.09 120 28 241 213 51.93 1.11 121 28 244 216 51.89 1.08 122 29 54 25 297.45 1.78 123 29 55 26 297.38 1.77 124 29 56 27 297.23 1.80 125 31 241 210 51.88 1.12 126 32 245 213 45.28 0.89 127 33 39 6 547.50 0.89 128 33 59 26 295.87 2.04 129 33 241 208 51.70 1.18 130 33 244 211 42.70 0.41 131 33 245 212 39.77 0.21 132 34 37 3 855.52 2.54 133 34 245 211 38.24 0.14 134 34 248 214 32.82 0.41 135 37 244 207 38.28 0.15 136 38 90 52 193.59 0.10 137 38 205 167 182.84 0.97 138 39 59 20 295.36 1.90 139 39 60 21 295.36 1.90 140 39 240 201 51.84 1.17 141 39 244 205 38.26 0.14 142 40 60 20 295.36 1.90 143 40 61 21 295.32 1.91 144 40 62 22 295.31 1.91 145 40 90 50 193.59 0.15 146 40 124 84 187.27 0.42 147 41 55 14 296.97 1.74 148 41 59 18 295.75 2.06 149 41 60 19 295.72 2.08 150 41 61 20 295.32 1.92 151 41 62 21 295.32 1.91 152 41 240 199 51.90 1.18 153 42 62 20 295.32 1.91 154 42 64 22 295.20 1.92 155 42 92 50 195.12 0.10 156 43 48 5 526.96 14.59 157 43 51 8 433.23 10.03 158 43 62 19 295.37 1.91 159 43 64 21 295.31 1.92 160 44 47 3 857.31 2.60 161 44 48 4 823.73 4.87 162 44 64 20 295.34 1.91 163 45 48 3 856.27 3.12 164 45 78 33 208.41 0.41 165 47 50 3 852.13 4.49 166 47 51 4 835.80 2.12 167 48 51 3 856.09 3.06 168 48 52 4 844.61 2.25 169 48 61 13 296.01 1.96 170 48 62 14 295.99 1.95 171 48 63 15 295.73 2.04 172 48 78 30 208.40 0.41 173 48 86 38 194.40 0.92 174 49 52 3 856.57 3.20 175 49 53 4 849.68 0.28 176 49 86 37 187.12 0.48 177 50 53 3 862.96 0.01 178 50 54 4 849.68 0.28 179 51 54 3 867.58 2.43 180 51 55 4 846.93 1.64 181 51 61 10 296.11 2.00 182 52 55 3 867.61 2.41 183 52 56 4 862.96 0.91 184 52 57 5 846.89 1.64 185 52 59 7 499.87 24.30 186 52 60 8 296.48 1.97 187 52 61 9 296.11 2.00 188 52 86 34 192.13 0.53 189 52 87 35 190.85 0.94 190 52 88 36 190.31 1.11 191 55 59 4 826.83 4.04 192 55 61 6 297.87 1.18 193 56 59 3 854.60 1.72 194 61 88 27 193.60 0.19 195 61 89 28 194.07 0.05 196 61 90 29 193.57 0.18 197 62 90 28 194.37 0.58 198 62 91 29 195.15 0.18 199 62 92 30 195.21 0.15 200 63 78 15 208.09 0.54 201 63 83 20 195.78 0.21 202 63 90 27 195.31 0.14 203 63 91 28 195.24 0.15 204 63 92 29 195.29 0.15 205 64 92 28 195.31 0.14 206 65 78 13 208.25 0.53 207 65 91 26 195.81 0.17 208 65 92 27 195.58 0.18 209 65 93 28 195.53 0.18 210 65 98 33 196.15 0.34 211 65 104 39 197.89 0.70 212 65 112 47 201.22 0.60 213 66 78 12 208.21 0.55 214 66 79 13 208.03 0.58 215 66 80 14 208.00 0.59 216 66 83 17 207.91 0.60 217 66 91 25 196.88 0.36 218 66 93 27 196.73 0.43 219 66 112 46 201.40 0.56 220 67 74 7 208.88 0.38 221 67 77 10 208.58 0.48 222 67 78 11 208.47 0.45 223 67 80 13 208.23 0.56 224 67 108 41 203.38 1.51 225 67 112 45 201.47 0.56 226 68 108 40 203.38 1.51 227 68 111 43 203.76 1.33 228 68 112 44 203.38 1.51 229 68 115 47 205.90 0.79 230 69 74 5 816.33 6.25 231 69 79 10 208.57 0.47 232 69 115 46 206.79 1.20 233 70 79 9 208.57 0.47 234 70 81 11 208.47 0.46 235 70 115 45 208.50 0.43 236 74 77 3 857.96 2.21 237 78 82 4 852.98 1.28 238 78 83 5 842.86 3.75 239 79 82 3 854.72 0.41 240 79 83 4 852.98 1.28 241 80 83 3 854.49 0.52 242 80 84 4 842.97 3.71 243 80 115 35 559.76 1.41 244 80 116 36 555.30 0.32 245 80 119 39 559.95 1.32 246 80 121 41 559.75 1.29 247 81 84 3 863.27 3.27 248 81 85 4 842.96 3.71 249 81 119 38 560.24 1.28 250 82 85 3 842.97 3.71 251 82 86 4 842.97 3.71 252 83 86 3 859.89 1.59 253 83 87 4 840.89 2.68 254 83 88 5 733.35 34.33 255 83 90 7 565.86 1.06 256 83 91 8 559.38 1.54 257 83 121 38 569.88 0.84 258 84 87 3 844.30 0.96 259 84 88 4 840.62 2.78 260 84 119 35 603.66 3.84 261 84 121 37 585.09 5.42 262 88 121 33 660.70 1.28 263 89 121 32 660.67 1.29 264 89 122 33 660.64 1.29 265 90 121 31 660.72 1.28 266 90 122 32 660.65 1.29 267 90 123 33 660.64 1.29 268 90 124 34 660.63 1.28 269 90 161 71 660.64 1.29 270 91 116 25 660.81 1.28 271 91 121 30 660.78 1.28 272 91 122 31 660.75 1.28 273 91 123 32 660.66 1.29 274 91 124 33 660.64 1.29 275 92 95 3 901.65 0.07 276 92 124 32 660.70 1.28 277 92 126 34 660.63 1.28 278 92 230 138 119.00 0.14 279 93 112 19 660.93 1.30 280 93 113 20 660.83 1.26 281 93 116 23 660.98 1.28 282 93 123 30 660.78 1.28 283 93 124 31 660.76 1.28 284 93 125 32 660.66 1.29 285 93 126 33 660.66 1.29 286 94 99 5 670.88 3.02 287 94 109 15 660.91 1.29 288 94 126 32 660.65 1.28 289 95 98 3 849.53 0.14 290 95 99 4 822.80 6.32 291 95 126 31 660.71 1.28 292 95 165 70 660.36 1.34 293 95 170 75 505.05 28.52 294 95 230 135 121.70 0.47 295 96 99 3 849.25 0.01 296 96 100 4 822.81 6.33 297 96 167 71 629.38 3.08 298 96 170 74 505.03 28.51 299 97 100 3 854.14 2.39 300 97 101 4 745.60 31.26 301 97 102 5 685.01 0.98 302 97 170 73 593.72 7.55 303 98 102 4 664.18 0.24 304 98 103 5 664.18 0.24 305 98 104 6 661.35 1.20 306 99 103 4 661.47 1.16 307 99 104 5 661.13 1.30 308 99 106 7 661.08 1.28 309 99 109 10 661.10 1.28 310 99 126 27 660.90 1.28 311 99 127 28 660.88 1.29 312 99 146 47 661.01 1.28 313 99 150 51 661.09 1.28 314 100 130 30 660.69 1.26 315 100 167 67 658.84 1.70 316 104 108 4 843.33 0.55 317 104 109 5 835.31 3.46 318 104 112 8 810.04 1.54 319 105 108 3 862.08 0.07 320 105 109 4 843.34 0.55 321 106 109 3 847.93 2.84 322 106 110 4 843.35 0.54 323 106 146 40 661.12 1.32 324 106 149 43 661.18 1.32 325 107 110 3 847.33 1.45 326 107 111 4 843.35 0.54 327 108 111 3 861.30 1.23 328 108 112 4 843.59 0.36 329 109 112 3 847.15 2.14 330 109 113 4 843.87 0.49 331 109 150 41 661.53 1.21 332 110 113 3 855.74 6.42 333 110 114 4 848.32 2.50 334 110 149 39 661.49 1.19 335 110 153 43 661.63 1.21 336 111 114 3 858.28 2.44 337 111 115 4 848.32 2.50 338 112 115 3 858.28 2.44 339 112 116 4 844.87 4.26 340 113 116 3 853.66 0.13 341 113 117 4 838.53 2.12 342 113 123 10 661.65 1.21 343 113 125 12 661.42 1.22 344 113 150 37 661.63 1.22 345 113 154 41 661.74 1.24 346 114 117 3 870.10 9.03 347 114 118 4 837.58 2.48 348 114 153 39 661.64 1.22 349 115 118 3 842.97 0.15 350 115 119 4 838.38 2.07 351 116 119 3 851.99 4.36 352 116 120 4 842.87 0.20 353 116 121 5 742.58 25.04 354 116 123 7 675.93 8.25 355 117 120 3 845.57 1.69 356 117 121 4 805.52 2.77 357 117 123 6 676.49 8.01 358 117 154 37 676.57 7.96 359 117 159 42 693.43 0.45 360 117 160 43 676.54 7.99 361 121 159 38 792.93 1.35 362 122 159 37 792.92 1.35 363 123 159 36 792.99 1.37 364 123 160 37 792.93 1.35 365 123 161 38 792.93 1.35 366 124 161 37 792.93 1.35 367 124 163 39 792.90 1.35 368 124 207 83 370.08 1.60 369 124 230 106 118.89 0.06 370 125 150 25 793.13 1.34 371 125 161 36 793.00 1.36 372 125 162 37 792.95 1.36 373 125 163 38 792.93 1.35 374 126 163 37 792.95 1.36 375 126 230 104 121.54 0.53 376 127 143 16 793.38 1.32 377 127 146 19 793.32 1.33 378 127 147 20 793.25 1.34 379 127 150 23 793.22 1.33 380 127 163 36 793.00 1.36 381 127 164 37 792.95 1.36 382 127 165 38 792.95 1.36 383 127 188 61 375.75 2.46 384 128 143 15 793.38 1.32 385 128 146 18 793.40 1.33 386 128 164 36 792.96 1.35 387 128 165 37 792.96 1.36 388 129 133 4 816.53 5.44 389 129 134 5 815.15 4.75 390 129 139 10 793.49 1.33 391 129 142 13 793.45 1.33 392 129 143 14 793.44 1.33 393 129 164 35 793.01 1.35 394 129 166 37 792.96 1.36 395 129 167 38 792.93 1.35 396 129 168 39 792.93 1.35 397 130 133 3 839.49 5.20 398 130 134 4 817.72 4.85 399 130 167 37 792.95 1.35 400 130 168 38 792.94 1.36 401 131 134 3 868.90 10.10 402 131 135 4 817.89 4.94 403 131 168 37 792.96 1.35 404 132 135 3 866.62 2.68 405 132 136 4 815.30 4.79 406 133 136 3 831.40 1.82 407 133 137 4 831.39 1.81 408 133 138 5 794.25 1.54 409 133 142 9 793.51 1.34 410 134 137 3 845.07 2.75 411 134 139 5 794.08 1.62 412 134 164 30 793.12 1.35 413 134 168 34 792.99 1.35 414 134 184 50 376.51 2.43 415 138 141 3 848.47 3.33 416 138 142 4 843.36 3.57 417 139 142 3 871.50 2.76 418 139 143 4 856.63 10.20 419 140 143 3 872.88 2.08 420 140 144 4 843.37 3.57 421 140 187 47 375.94 2.51 422 141 144 3 872.88 2.08 423 141 145 4 844.34 3.08 424 142 145 3 864.19 6.42 425 142 146 4 848.07 1.65 426 143 146 3 848.08 1.64 427 143 147 4 848.07 1.65 428 143 164 21 793.02 1.35 429 143 188 45 375.85 2.51 430 144 147 3 853.52 1.08 431 144 148 4 839.22 6.07 432 144 187 43 375.92 2.52 433 144 188 44 375.85 2.50 434 144 191 47 375.61 2.50 435 145 148 3 860.61 2.44 436 145 149 4 855.38 0.17 437 146 149 3 857.79 1.36 438 146 150 4 855.38 0.17 439 147 150 3 855.38 0.17 440 147 151 4 839.24 6.03 441 147 162 15 793.05 1.34 442 147 188 41 375.83 2.50 443 147 191 44 375.50 2.54 444 148 151 3 843.71 5.67 445 148 152 4 827.51 0.17 446 148 191 43 375.48 2.56 447 149 153 4 845.91 9.04 448 150 153 3 873.46 2.68 449 150 154 4 827.86 0.01 450 150 162 12 793.04 1.34 451 151 154 3 843.44 1.25 452 151 157 6 794.24 1.51 453 151 191 40 375.46 2.56 454 151 196 45 371.66 1.57 455 154 157 3 886.17 3.91 456 154 160 6 793.32 1.48 457 156 160 4 860.85 2.54 458 157 160 3 860.69 2.62 459 157 192 35 374.76 2.29 460 157 196 39 371.73 1.61 461 157 200 43 371.01 1.75 462 157 204 47 370.86 1.71 463 161 205 44 370.31 1.52 464 161 207 46 370.14 1.57 465 162 192 30 373.75 1.79 466 162 204 42 370.97 1.69 467 162 205 43 370.41 1.54 468 162 206 44 370.17 1.58 469 162 207 45 370.16 1.58 470 163 207 44 370.15 1.57 471 163 230 67 121.28 0.40 472 164 176 12 376.48 2.56 473 164 184 20 375.75 2.46 474 164 185 21 375.74 2.46 475 164 188 24 375.70 2.45 476 164 207 43 370.34 1.51 477 164 208 44 370.22 1.54 478 164 209 45 370.08 1.57 479 165 170 5 800.00 3.90 480 165 209 44 370.01 1.59 481 165 230 65 121.83 0.45 482 166 169 3 859.49 8.05 483 166 170 4 841.91 0.73 484 166 176 10 376.64 2.48 485 166 208 42 370.26 1.54 486 166 209 43 370.22 1.53 487 167 170 3 843.92 1.41 488 168 172 4 839.02 0.71 489 168 174 6 419.48 0.54 490 168 176 8 394.69 6.19 491 169 172 3 846.65 2.38 492 169 174 5 408.09 5.60 493 169 210 41 369.72 1.71 494 169 211 42 369.72 1.71 495 173 211 38 369.92 1.64 496 174 211 37 370.17 1.52 497 175 208 33 370.78 1.58 498 175 210 35 370.24 1.52 499 175 211 36 370.19 1.53 500 175 216 41 352.71 0.32 501 176 180 4 812.78 1.48 502 176 181 5 809.10 3.31 503 176 208 32 370.83 1.60 504 177 180 3 835.56 1.49 505 177 181 4 827.72 2.52 506 178 181 3 853.86 0.51 507 178 182 4 843.82 5.53 508 178 219 41 221.86 2.69 509 178 220 42 173.97 21.25 510 178 223 45 126.59 2.27 511 179 182 3 853.87 0.51 512 179 183 4 843.84 5.52 513 180 183 3 857.87 1.48 514 180 184 4 843.84 5.53 515 181 184 3 859.17 2.13 516 181 185 4 854.84 0.04 517 181 208 27 370.98 1.68 518 181 220 39 173.89 21.27 519 181 226 45 122.73 0.49 520 182 185 3 853.73 0.59 521 182 186 4 843.82 5.54 522 182 223 41 122.78 0.49 523 182 224 42 122.80 0.47 524 182 225 43 122.77 0.48 525 183 186 3 858.15 1.61 526 183 187 4 853.73 0.59 527 184 187 3 853.73 0.60 528 184 188 4 838.03 7.25 529 185 188 3 853.60 0.54 530 185 189 4 826.65 2.08 531 185 206 21 371.18 1.66 532 185 207 22 371.18 1.66 533 185 208 23 371.06 1.64 534 185 225 40 122.71 0.48 535 185 226 41 122.70 0.49 536 186 189 3 861.21 3.27 537 186 190 4 835.50 2.33 538 186 225 39 122.72 0.48 539 187 190 3 855.99 5.88 540 187 191 4 835.52 2.33 541 188 191 3 853.96 6.88 542 188 192 4 836.52 1.84 543 188 206 18 371.25 1.62 544 189 192 3 847.99 3.89 545 189 193 4 839.17 0.52 546 189 201 12 371.83 1.66 547 189 204 15 371.55 1.54 548 189 206 17 371.26 1.63 549 189 225 36 122.65 0.47 550 189 227 38 122.61 0.49 551 190 193 3 848.40 2.28 552 190 194 4 838.36 2.75 553 191 194 3 864.35 5.70 554 191 195 4 838.35 2.75 555 191 196 5 837.38 3.24 556 192 195 3 856.14 5.78 557 192 196 4 827.04 1.92 558 192 197 5 822.96 0.14 559 192 200 8 411.36 0.02 560 192 201 9 371.81 1.65 561 192 204 12 371.52 1.54 562 192 206 14 371.29 1.62 563 193 197 4 817.24 3.00 564 193 198 5 438.73 3.55 565 193 201 8 371.89 1.66 566 196 200 4 833.59 3.12 567 197 200 3 852.05 4.35 568 197 201 4 849.06 5.84 569 198 201 3 858.87 0.95 570 198 202 4 849.04 5.83 571 199 202 3 861.18 0.21 572 199 203 4 827.26 0.04 573 200 203 3 847.57 6.59 574 200 204 4 824.83 1.18 575 204 227 23 122.24 0.36 576 205 227 22 122.23 0.36 577 206 225 19 122.45 0.41 578 206 226 20 122.31 0.39 579 206 227 21 122.24 0.36 580 207 227 20 122.24 0.36 581 207 228 21 121.98 0.42 582 207 229 22 121.93 0.40 583 207 230 23 121.91 0.38 584 208 212 4 873.38 8.61 585 208 220 12 122.58 0.52 586 208 226 18 122.43 0.40 587 208 228 20 122.25 0.36 588 208 229 21 121.94 0.40 589 208 230 22 121.94 0.40 590 209 212 3 886.59 3.93 591 209 230 21 121.81 0.43 592 211 216 5 823.58 0.29 593 212 216 4 827.15 2.08 594 212 217 5 572.70 63.71 595 212 220 8 425.70 6.06 596 212 229 17 122.41 0.38 597 212 231 19 122.24 0.38 598 212 234 22 121.75 0.46 599 212 243 31 48.34 1.18 600 213 216 3 856.64 7.58 601 213 217 4 822.26 0.36 602 213 234 21 121.82 0.45 603 213 239 26 52.92 0.65 604 213 243 30 49.80 1.85 605 214 217 3 851.53 0.69 606 214 239 25 52.91 0.65 607 214 243 29 48.39 1.15 608 216 219 3 867.05 4.02 609 216 220 4 837.20 2.01 610 217 220 3 840.38 3.27 611 217 221 4 826.90 0.53 612 217 229 12 122.70 0.46 613 217 243 26 46.79 1.71 614 217 246 29 35.52 1.73 615 218 221 3 849.99 1.03 616 218 222 4 841.25 2.46 617 218 246 28 35.49 1.72 618 218 248 30 35.39 1.68 619 219 222 3 858.88 0.59 620 219 223 4 853.75 0.77 621 220 223 3 861.62 1.97 622 220 226 6 260.69 68.45 623 220 229 9 122.79 0.48 624 221 226 5 260.70 68.46 625 221 229 8 122.78 0.48 626 221 246 25 35.29 1.62 627 221 247 26 34.88 1.74 628 223 226 3 835.17 5.67 629 226 229 3 974.79 1.80 630 229 243 14 40.26 0.09 631 229 246 17 35.30 1.63 632 231 234 3 883.72 2.94 633 231 235 4 827.29 1.58 634 231 240 9 51.87 1.18 635 231 243 12 43.61 0.04 636 232 235 3 866.76 4.13 637 233 236 3 882.23 4.38 638 233 237 4 856.62 0.94 639 234 237 3 843.80 1.91 640 234 239 5 621.77 94.17 641 234 240 6 379.37 3.06 642 234 243 9 213.72 79.77 643 235 240 5 381.38 2.18 644 237 240 3 869.60 1.90 645 238 241 3 869.60 1.91 646 239 242 3 862.33 1.25 647 239 243 4 856.41 0.18 648 240 243 3 856.00 0.03 649 240 244 4 843.98 6.04 650 241 244 3 843.98 6.04 651 241 245 4 840.45 7.80 652 242 245 3 840.38 7.83 653 242 246 4 823.49 0.60 654 243 246 3 823.45 0.58 655 245 248 3 887.20 1.12