BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 172.80 0.52 2 4 204 200 171.13 0.18 3 4 205 201 164.63 1.38 4 4 224 220 118.71 2.37 5 4 225 221 118.71 2.37 6 4 227 223 118.45 2.40 7 5 35 30 548.86 0.60 8 5 36 31 548.81 0.61 9 5 38 33 548.75 0.60 10 5 205 200 178.00 1.89 11 6 36 30 548.84 0.60 12 6 37 31 548.77 0.60 13 6 38 32 548.77 0.61 14 6 221 215 118.70 2.36 15 6 227 221 118.67 2.38 16 6 228 222 118.64 2.38 17 6 229 223 118.65 2.38 18 6 246 240 34.12 0.07 19 6 247 241 28.15 0.83 20 7 38 31 548.78 0.60 21 7 40 33 548.78 0.61 22 7 90 83 356.54 1.38 23 7 124 117 334.69 0.80 24 7 161 154 333.99 0.53 25 7 205 198 185.23 0.20 26 7 207 200 172.69 0.78 27 7 227 220 118.80 2.35 28 7 228 221 118.67 2.37 29 7 229 222 118.65 2.38 30 8 37 29 548.86 0.61 31 8 38 30 548.84 0.61 32 8 39 31 548.79 0.60 33 8 40 32 548.78 0.61 34 8 229 221 118.66 2.38 35 8 231 223 118.62 2.38 36 8 240 232 53.72 1.20 37 8 243 235 43.88 0.34 38 8 244 236 35.31 0.18 39 9 40 31 548.78 0.61 40 9 229 220 118.92 2.33 41 9 230 221 118.65 2.38 42 9 231 222 118.64 2.38 43 9 240 231 53.77 1.20 44 10 40 30 548.84 0.61 45 10 41 31 548.79 0.60 46 10 42 32 548.77 0.61 47 10 231 221 118.66 2.38 48 10 235 225 76.19 0.03 49 10 240 230 53.77 1.20 50 11 42 31 548.79 0.60 51 11 64 53 408.04 23.91 52 11 92 81 357.37 0.97 53 11 95 84 357.25 0.90 54 11 230 219 119.42 2.14 55 11 231 220 118.87 2.29 56 11 232 221 118.65 2.38 57 11 235 224 86.11 0.97 58 12 15 3 873.13 5.62 59 12 21 9 550.39 0.35 60 12 24 12 549.79 0.65 61 12 25 13 549.79 0.64 62 12 41 29 548.87 0.61 63 12 42 30 548.82 0.61 64 12 43 31 548.81 0.61 65 12 44 32 548.80 0.61 66 12 51 39 470.69 0.15 67 12 64 52 408.36 24.04 68 12 235 223 104.06 2.23 69 12 236 224 85.34 1.05 70 13 44 31 548.79 0.62 71 13 64 51 408.24 24.03 72 13 95 82 357.81 1.17 73 13 97 84 357.47 0.95 74 13 232 219 119.38 2.16 75 15 21 6 688.35 58.22 76 15 44 29 548.86 0.59 77 15 236 221 118.58 2.41 78 16 20 4 813.83 4.53 79 16 21 5 688.35 58.22 80 16 47 31 548.75 0.59 81 17 20 3 845.02 6.53 82 17 21 4 814.21 4.71 83 17 47 30 548.75 0.59 84 18 22 4 815.91 5.38 85 18 47 29 548.63 0.53 86 18 50 32 514.42 0.30 87 19 22 3 850.23 4.97 88 19 23 4 855.46 3.07 89 20 23 3 855.46 3.07 90 20 24 4 854.74 2.71 91 21 24 3 855.45 3.08 92 21 25 4 839.61 4.84 93 21 44 23 548.92 0.59 94 21 47 26 548.50 0.48 95 21 50 29 500.62 1.18 96 22 25 3 863.30 0.84 97 22 26 4 853.05 4.28 98 22 50 28 500.62 1.18 99 23 26 3 853.04 4.27 100 23 27 4 852.59 4.50 101 24 27 3 857.35 2.12 102 24 28 4 854.59 3.50 103 24 235 211 117.20 2.98 104 24 236 212 84.95 1.25 105 25 28 3 853.15 0.88 106 25 29 4 845.69 2.85 107 25 50 25 494.40 1.84 108 25 51 26 475.43 0.94 109 25 54 29 411.57 23.80 110 25 55 30 411.54 23.81 111 26 29 3 861.47 3.29 112 26 30 4 845.75 2.83 113 27 30 3 862.50 3.80 114 27 31 4 842.58 1.90 115 27 238 211 69.52 0.09 116 28 31 3 852.62 4.01 117 28 33 5 812.75 2.88 118 28 41 13 549.00 0.60 119 28 55 27 411.54 23.81 120 28 59 31 409.99 24.12 121 28 240 212 58.68 1.09 122 28 241 213 53.74 1.21 123 28 244 216 52.88 1.61 124 29 54 25 411.56 23.80 125 29 55 26 411.54 23.81 126 29 56 27 411.50 23.82 127 29 59 30 410.36 24.00 128 31 241 210 53.71 1.21 129 32 245 213 45.73 0.01 130 33 39 6 549.16 0.55 131 33 58 25 409.00 24.04 132 33 59 26 408.95 24.06 133 33 241 208 53.05 1.47 134 33 244 211 44.79 0.03 135 33 245 212 43.96 0.41 136 34 37 3 855.55 3.31 137 34 244 210 44.03 0.39 138 34 245 211 40.91 0.05 139 34 248 214 31.23 0.36 140 36 248 212 30.69 0.08 141 37 244 207 40.74 0.04 142 38 90 52 357.70 1.29 143 38 205 167 185.45 0.28 144 39 59 20 408.54 23.98 145 39 60 21 408.53 23.98 146 39 240 201 53.75 1.20 147 39 244 205 38.94 0.86 148 40 59 19 408.64 23.96 149 40 60 20 408.53 23.99 150 40 61 21 408.49 24.00 151 40 62 22 408.48 24.00 152 40 90 50 357.76 1.31 153 40 124 84 355.06 0.86 154 41 55 14 410.39 24.35 155 41 59 18 408.86 24.04 156 41 60 19 408.65 23.96 157 41 61 20 408.53 23.98 158 41 62 21 408.49 24.00 159 42 62 20 408.45 24.02 160 42 64 22 408.19 23.95 161 42 92 50 357.83 1.22 162 43 48 5 524.53 13.38 163 43 51 8 456.31 1.54 164 43 62 19 408.62 23.96 165 43 64 21 408.43 24.03 166 44 47 3 860.87 0.07 167 44 48 4 767.15 34.41 168 44 64 20 408.43 24.05 169 45 48 3 854.76 3.12 170 45 78 33 364.75 2.46 171 47 50 3 853.63 4.28 172 47 51 4 840.59 2.25 173 48 51 3 857.66 2.69 174 48 52 4 847.49 1.21 175 48 61 13 409.11 24.06 176 48 62 14 409.05 24.09 177 48 63 15 408.95 24.07 178 48 78 30 365.62 2.02 179 48 83 35 361.94 0.54 180 48 86 38 363.37 0.18 181 49 52 3 861.10 3.14 182 49 53 4 848.72 1.82 183 49 86 37 363.36 0.18 184 50 53 3 857.14 2.12 185 50 54 4 847.49 1.22 186 51 54 3 855.44 3.39 187 51 55 4 847.33 1.15 188 51 61 10 409.67 23.84 189 52 55 3 855.60 3.31 190 52 56 4 869.27 3.53 191 52 57 5 846.53 0.77 192 52 59 7 630.99 18.80 193 52 60 8 411.17 23.71 194 52 61 9 409.72 23.81 195 52 86 34 363.38 0.20 196 52 87 35 362.22 0.77 197 52 88 36 359.64 2.04 198 55 59 4 826.90 10.40 199 55 61 6 444.42 7.55 200 56 59 3 874.47 6.55 201 61 88 27 390.37 17.49 202 61 89 28 390.37 17.49 203 61 90 29 390.16 17.45 204 62 90 28 390.16 17.45 205 62 91 29 390.25 17.47 206 62 92 30 390.23 17.41 207 62 124 62 389.56 17.73 208 63 78 15 398.94 14.65 209 63 83 20 394.23 15.79 210 63 90 27 390.44 17.46 211 63 91 28 390.37 17.50 212 63 92 29 390.38 17.48 213 64 92 28 390.44 17.52 214 65 78 13 399.21 14.61 215 65 91 26 390.79 17.50 216 65 92 27 390.73 17.52 217 65 93 28 390.73 17.51 218 65 98 33 390.70 17.50 219 65 104 39 391.10 17.64 220 65 112 47 394.63 15.96 221 66 78 12 399.21 14.61 222 66 79 13 399.04 14.69 223 66 80 14 398.97 14.68 224 66 83 17 398.93 14.70 225 66 91 25 393.28 16.47 226 66 93 27 390.91 17.55 227 66 112 46 394.76 15.94 228 67 74 7 399.43 14.67 229 67 77 10 399.31 14.64 230 67 78 11 399.29 14.63 231 67 80 13 399.14 14.64 232 67 108 41 394.81 15.93 233 67 112 45 395.02 16.03 234 67 115 48 396.19 16.11 235 68 108 40 394.82 15.92 236 68 111 43 394.82 15.92 237 68 112 44 394.81 15.93 238 68 115 47 395.68 16.35 239 69 74 5 753.47 33.30 240 69 79 10 399.39 14.68 241 69 115 46 396.40 16.02 242 70 79 9 399.39 14.69 243 70 81 11 399.38 14.69 244 70 115 45 397.23 15.74 245 74 77 3 880.94 1.45 246 78 82 4 847.43 4.03 247 78 83 5 842.88 6.28 248 79 82 3 856.52 1.54 249 79 83 4 854.46 0.51 250 80 83 3 856.15 1.35 251 80 84 4 856.61 0.58 252 80 115 35 554.98 1.00 253 80 116 36 474.73 40.68 254 80 119 39 559.19 0.97 255 80 121 41 559.12 0.98 256 81 84 3 856.62 0.57 257 81 85 4 856.61 0.57 258 81 119 38 565.70 4.21 259 82 85 3 856.61 0.57 260 82 86 4 856.61 0.58 261 83 86 3 868.43 0.06 262 83 87 4 856.74 0.51 263 83 88 5 747.60 27.08 264 83 90 7 620.33 23.29 265 83 91 8 561.12 2.04 266 83 121 38 578.76 2.02 267 84 87 3 870.33 6.32 268 84 88 4 825.28 4.16 269 84 119 35 595.36 0.29 270 84 121 37 578.83 2.02 271 88 121 33 663.01 0.34 272 89 121 32 663.01 0.34 273 89 122 33 662.99 0.34 274 90 121 31 663.02 0.34 275 90 122 32 663.00 0.34 276 90 123 33 663.00 0.34 277 90 124 34 662.98 0.34 278 90 159 69 663.01 0.34 279 90 161 71 663.00 0.34 280 90 205 115 204.69 7.68 281 91 116 25 663.15 0.35 282 91 121 30 663.14 0.35 283 91 122 31 663.12 0.34 284 91 123 32 663.01 0.34 285 91 124 33 663.00 0.34 286 92 95 3 901.91 0.20 287 92 124 32 663.01 0.34 288 92 126 34 662.99 0.34 289 92 230 138 119.80 1.94 290 93 112 19 663.28 0.33 291 93 113 20 663.16 0.35 292 93 116 23 663.29 0.34 293 93 123 30 663.15 0.35 294 93 124 31 663.12 0.35 295 93 125 32 663.04 0.36 296 93 126 33 663.00 0.34 297 94 99 5 742.58 33.99 298 94 109 15 663.16 0.36 299 94 126 32 663.02 0.35 300 95 98 3 853.62 3.66 301 95 99 4 834.01 4.25 302 95 126 31 663.04 0.36 303 95 165 70 662.99 0.34 304 95 230 135 121.89 0.93 305 96 99 3 848.23 0.24 306 96 100 4 827.59 1.04 307 96 165 69 662.99 0.34 308 96 167 71 656.96 0.78 309 97 100 3 848.75 0.33 310 97 101 4 823.09 3.29 311 97 102 5 820.16 4.75 312 98 102 4 740.98 37.72 313 98 103 5 670.08 2.27 314 98 104 6 664.39 0.21 315 99 103 4 664.41 0.21 316 99 104 5 664.40 0.21 317 99 106 7 664.40 0.21 318 99 109 10 664.38 0.21 319 99 126 27 663.25 0.32 320 99 127 28 663.23 0.32 321 99 146 47 663.28 0.33 322 104 108 4 859.26 2.77 323 104 109 5 827.67 0.93 324 104 112 8 759.38 31.85 325 105 108 3 859.26 2.77 326 105 109 4 853.80 5.50 327 106 109 3 862.62 1.09 328 106 110 4 859.25 2.77 329 106 146 40 663.28 0.34 330 106 149 43 663.36 0.32 331 107 110 3 859.25 2.77 332 107 111 4 859.25 2.77 333 108 111 3 856.85 3.46 334 108 112 4 830.34 1.65 335 109 112 3 855.00 2.54 336 109 113 4 840.31 1.84 337 109 150 41 663.46 0.32 338 110 113 3 850.19 0.84 339 110 114 4 839.72 1.55 340 110 149 39 663.45 0.34 341 110 153 43 663.63 0.38 342 111 114 3 862.76 1.98 343 111 115 4 840.71 1.82 344 112 115 3 863.91 1.40 345 112 116 4 839.60 1.48 346 113 116 3 855.45 0.85 347 113 117 4 848.26 2.74 348 113 123 10 663.65 0.40 349 113 150 37 663.50 0.32 350 113 153 40 663.92 0.37 351 113 154 41 663.95 0.36 352 114 117 3 860.75 0.47 353 114 118 4 841.02 4.03 354 114 153 39 663.91 0.37 355 115 118 3 850.78 4.51 356 115 119 4 840.09 4.49 357 116 119 3 860.53 0.09 358 116 120 4 841.03 4.03 359 116 121 5 752.34 32.66 360 116 123 7 675.13 5.04 361 117 120 3 855.90 6.16 362 117 121 4 809.67 4.00 363 117 123 6 676.05 5.44 364 117 154 37 698.12 0.91 365 117 159 42 697.12 1.41 366 117 160 43 678.84 4.06 367 120 159 39 797.51 1.31 368 121 159 38 797.51 1.31 369 122 159 37 797.51 1.31 370 123 159 36 797.56 1.32 371 123 160 37 797.52 1.31 372 123 161 38 797.51 1.31 373 124 161 37 797.51 1.31 374 124 163 39 797.48 1.30 375 124 207 83 204.24 7.81 376 124 230 106 119.69 1.94 377 125 150 25 797.72 1.32 378 125 161 36 797.56 1.31 379 125 162 37 797.52 1.31 380 125 163 38 797.50 1.31 381 126 163 37 797.51 1.32 382 126 165 39 797.47 1.30 383 126 230 104 121.73 0.97 384 127 143 16 797.99 1.28 385 127 146 19 797.89 1.27 386 127 147 20 797.88 1.28 387 127 150 23 797.88 1.28 388 127 162 35 797.59 1.31 389 127 163 36 797.55 1.31 390 127 164 37 797.50 1.31 391 127 165 38 797.50 1.30 392 127 188 61 245.16 1.09 393 128 143 15 798.10 1.26 394 128 146 18 798.03 1.24 395 128 164 36 797.53 1.31 396 128 165 37 797.50 1.31 397 128 167 39 797.42 1.32 398 129 133 4 828.39 3.93 399 129 134 5 811.81 3.19 400 129 139 10 798.10 1.25 401 129 142 13 798.12 1.26 402 129 143 14 798.10 1.26 403 129 164 35 797.59 1.32 404 129 167 38 797.47 1.31 405 129 168 39 797.44 1.32 406 130 133 3 841.48 0.09 407 130 134 4 831.22 2.54 408 130 167 37 797.48 1.31 409 130 168 38 797.45 1.31 410 131 134 3 850.75 6.40 411 131 135 4 833.92 3.90 412 131 168 37 797.46 1.32 413 131 172 41 727.99 0.68 414 131 174 43 676.03 2.58 415 132 135 3 843.76 1.01 416 132 136 4 833.92 3.91 417 133 136 3 847.59 7.98 418 133 137 4 833.91 3.91 419 133 138 5 798.53 1.15 420 133 142 9 798.21 1.28 421 134 137 3 839.90 0.91 422 134 139 5 798.52 1.15 423 134 164 30 797.66 1.30 424 134 168 34 797.50 1.32 425 134 180 46 248.01 1.19 426 134 184 50 247.49 1.31 427 138 141 3 862.50 6.46 428 138 142 4 839.10 1.19 429 139 142 3 867.66 3.88 430 139 143 4 839.16 1.16 431 139 184 45 246.00 1.27 432 140 143 3 860.48 0.29 433 140 144 4 839.13 1.12 434 140 187 47 245.61 1.13 435 141 144 3 854.74 2.58 436 141 145 4 839.36 1.22 437 142 145 3 847.62 2.62 438 142 146 4 840.83 1.96 439 143 146 3 850.72 2.98 440 143 147 4 840.84 1.97 441 143 164 21 797.60 1.32 442 143 188 45 245.46 1.15 443 144 147 3 844.65 0.10 444 144 148 4 832.25 6.30 445 144 187 43 245.59 1.12 446 144 188 44 245.57 1.14 447 144 191 47 245.16 1.08 448 145 148 3 859.35 0.07 449 145 149 4 847.34 1.24 450 146 149 3 857.59 0.95 451 146 150 4 852.76 1.48 452 147 150 3 857.60 0.94 453 147 151 4 847.33 1.25 454 147 162 15 797.62 1.32 455 147 188 41 245.41 1.18 456 147 191 44 245.16 1.09 457 147 192 45 245.13 1.08 458 148 151 3 847.33 1.25 459 148 152 4 847.32 1.25 460 148 191 43 245.22 1.11 461 149 153 4 847.56 3.46 462 150 153 3 872.19 8.86 463 150 154 4 837.37 6.23 464 150 162 12 797.56 1.31 465 151 154 3 849.14 0.83 466 151 157 6 798.59 1.43 467 151 191 40 245.16 1.09 468 151 196 45 212.39 4.95 469 154 157 3 876.50 3.11 470 154 160 6 798.40 1.28 471 156 160 4 856.45 3.81 472 157 160 3 846.56 2.14 473 157 162 5 845.84 2.49 474 157 192 35 243.61 0.95 475 157 196 39 206.75 7.86 476 157 200 43 205.61 7.49 477 157 204 47 204.98 7.79 478 160 204 44 204.69 7.67 479 161 205 44 204.40 7.82 480 161 207 46 204.21 7.79 481 162 192 30 243.62 0.96 482 162 205 43 204.84 7.75 483 162 206 44 204.26 7.82 484 162 207 45 204.21 7.79 485 163 207 44 204.21 7.79 486 163 230 67 121.67 0.95 487 164 184 20 245.16 1.07 488 164 185 21 245.12 1.07 489 164 188 24 245.12 1.07 490 164 207 43 204.49 7.78 491 164 208 44 204.19 7.78 492 164 209 45 204.16 7.79 493 165 209 44 203.28 7.36 494 166 169 3 873.19 1.21 495 166 170 4 835.87 3.02 496 166 176 10 245.94 1.26 497 166 208 42 204.50 7.78 498 166 209 43 204.28 7.74 499 166 210 44 203.28 7.35 500 167 170 3 857.03 9.29 501 168 172 4 830.91 5.50 502 168 174 6 273.30 2.60 503 168 175 7 248.43 1.36 504 168 176 8 248.43 1.36 505 168 180 12 247.99 1.20 506 169 172 3 869.49 3.06 507 169 174 5 248.93 1.10 508 169 175 6 248.36 1.39 509 169 176 7 248.30 1.32 510 176 180 4 840.34 1.07 511 176 208 32 205.97 7.61 512 177 180 3 858.34 1.76 513 177 181 4 848.77 3.14 514 178 181 3 858.46 1.70 515 178 182 4 848.80 3.15 516 178 219 41 122.88 0.53 517 178 220 42 122.88 0.53 518 178 223 45 122.67 0.64 519 179 182 3 858.76 1.55 520 179 183 4 848.80 3.15 521 180 183 3 845.29 4.91 522 180 184 4 858.52 1.71 523 181 184 3 864.49 1.11 524 181 185 4 858.51 1.71 525 181 208 27 206.06 7.58 526 181 220 39 122.88 0.53 527 182 185 3 862.59 3.73 528 182 186 4 850.46 2.35 529 182 223 41 122.55 0.66 530 182 224 42 122.59 0.64 531 182 225 43 122.55 0.64 532 183 186 3 864.96 2.54 533 183 187 4 858.75 1.76 534 184 187 3 858.76 1.75 535 184 188 4 842.43 6.42 536 185 188 3 862.63 3.69 537 185 189 4 847.90 3.69 538 185 206 21 206.09 7.61 539 185 207 22 206.09 7.61 540 185 208 23 206.07 7.60 541 185 225 40 122.45 0.66 542 185 226 41 122.42 0.68 543 186 189 3 867.14 1.43 544 186 190 4 855.77 0.24 545 186 225 39 122.42 0.64 546 187 190 3 855.78 0.24 547 187 191 4 855.77 0.24 548 188 191 3 860.77 2.73 549 188 192 4 860.79 2.74 550 188 206 18 206.09 7.60 551 189 192 3 860.81 2.74 552 189 193 4 854.62 5.83 553 189 201 12 206.72 7.71 554 189 204 15 206.47 7.71 555 189 206 17 206.10 7.60 556 189 225 36 122.37 0.67 557 189 227 38 122.41 0.68 558 190 193 3 860.80 2.75 559 190 194 4 854.62 5.83 560 191 194 3 860.81 2.74 561 191 195 4 854.60 5.83 562 191 196 5 832.36 5.29 563 192 196 4 816.96 2.35 564 192 197 5 691.74 60.26 565 192 200 8 235.80 6.67 566 192 201 9 206.70 7.72 567 192 204 12 206.27 7.65 568 192 206 14 206.10 7.60 569 193 197 4 275.43 7.38 570 193 198 5 235.85 6.69 571 193 201 8 206.73 7.73 572 196 200 4 843.29 7.98 573 197 200 3 865.51 3.14 574 197 201 4 843.30 7.98 575 198 201 3 864.25 4.79 576 198 202 4 843.30 7.98 577 199 202 3 847.20 6.04 578 199 203 4 843.28 7.98 579 200 203 3 860.13 2.35 580 200 204 4 822.04 0.04 581 205 227 22 121.94 0.86 582 206 225 19 122.39 0.71 583 206 226 20 122.14 0.82 584 206 227 21 121.99 0.85 585 207 227 20 122.04 0.88 586 207 228 21 121.91 0.89 587 207 229 22 121.89 0.90 588 207 230 23 121.89 0.90 589 208 212 4 872.56 4.08 590 208 220 12 122.48 0.72 591 208 226 18 122.33 0.74 592 208 228 20 122.13 0.83 593 208 229 21 121.90 0.90 594 208 230 22 121.89 0.89 595 209 230 21 121.86 0.91 596 211 216 5 838.16 4.13 597 212 216 4 838.16 4.13 598 212 217 5 706.01 61.94 599 212 229 17 122.36 0.75 600 212 231 19 122.06 0.83 601 212 234 22 117.66 1.26 602 212 243 31 49.62 0.62 603 213 216 3 853.38 0.48 604 213 217 4 826.47 6.12 605 213 234 21 112.22 3.98 606 213 239 26 55.38 0.39 607 213 243 30 50.52 0.40 608 214 217 3 869.47 5.29 609 214 239 25 55.29 0.37 610 214 242 28 53.52 1.09 611 214 243 29 51.27 0.02 612 215 219 4 879.46 0.29 613 216 219 3 878.84 0.01 614 216 220 4 851.65 0.53 615 217 220 3 855.62 0.51 616 217 221 4 841.16 4.72 617 217 229 12 122.51 0.74 618 217 243 26 44.46 0.29 619 217 246 29 35.12 0.12 620 218 221 3 863.95 3.66 621 218 222 4 830.84 0.45 622 218 246 28 35.12 0.12 623 219 222 3 854.95 1.29 624 219 223 4 847.61 2.38 625 220 223 3 854.91 1.28 626 220 226 6 122.95 0.63 627 220 229 9 122.59 0.70 628 221 226 5 122.83 0.73 629 221 229 8 122.62 0.72 630 221 246 25 34.52 0.01 631 221 247 26 34.20 0.08 632 223 226 3 838.46 2.34 633 226 229 3 984.03 0.79 634 229 243 14 43.90 0.33 635 229 246 17 34.69 0.10 636 231 234 3 890.11 1.10 637 231 235 4 837.66 8.42 638 231 240 9 87.11 17.84 639 231 243 12 45.55 0.80 640 232 235 3 871.90 1.68 641 233 236 3 875.95 0.35 642 233 237 4 868.18 2.65 643 234 237 3 868.17 2.64 644 234 239 5 542.66 135.17 645 234 240 6 186.06 30.24 646 234 243 9 125.08 0.25 647 235 240 5 140.24 44.41 648 237 240 3 862.69 1.86 649 238 241 3 878.83 3.09 650 239 242 3 861.49 2.28 651 239 243 4 844.97 6.45 652 240 243 3 856.91 0.48 653 240 244 4 825.57 3.25 654 241 244 3 845.00 6.46 655 241 245 4 844.97 6.45 656 242 245 3 845.32 6.31 657 242 246 4 830.25 0.92 658 243 246 3 835.68 1.81 659 245 248 3 858.19 0.22