BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 21 224 203 57.91 1.40 2 21 225 204 57.85 1.37 3 21 226 205 57.86 1.38 4 21 229 208 57.85 1.37 5 22 31 9 57.85 1.38 6 22 33 11 57.87 1.38 7 23 29 6 389.61 2.94 8 23 30 7 57.94 1.40 9 23 31 8 57.91 1.39 10 23 223 200 58.01 1.41 11 24 30 6 62.73 0.98 12 24 32 8 58.01 1.42 13 24 58 34 57.95 1.42 14 25 28 3 817.11 2.73 15 25 29 4 752.96 4.69 16 26 57 31 58.01 1.42 17 26 58 32 57.95 1.42 18 27 58 31 58.02 1.41 19 27 59 32 57.97 1.41 20 27 234 207 58.00 1.41 21 28 219 191 58.14 1.43 22 28 234 206 58.01 1.41 23 28 237 209 58.02 1.41 24 29 218 189 58.16 1.43 25 29 219 190 58.16 1.43 26 29 223 194 64.66 1.09 27 29 232 203 58.09 1.44 28 30 58 28 58.61 1.24 29 30 231 201 58.28 1.48 30 30 232 202 58.13 1.45 31 31 223 192 64.72 1.06 32 31 225 194 64.81 1.07 33 31 230 199 62.02 0.01 34 31 231 200 60.27 0.49 35 32 37 5 805.81 2.76 36 32 53 21 64.53 1.02 37 32 229 197 64.81 1.07 38 32 230 198 64.32 1.14 39 33 36 3 860.52 1.14 40 33 37 4 828.77 1.23 41 33 229 196 64.84 1.06 42 34 37 3 842.19 1.35 43 34 228 194 65.55 0.89 44 37 51 14 92.68 0.57 45 37 53 16 83.02 0.40 46 37 66 29 92.00 0.96 47 38 51 13 93.16 0.53 48 39 49 10 96.26 0.49 49 39 50 11 96.03 0.41 50 40 50 10 96.26 0.49 51 40 51 11 96.23 0.49 52 40 52 12 96.20 0.48 53 42 45 3 852.48 1.41 54 42 50 8 106.78 2.29 55 42 52 10 99.00 0.23 56 42 65 23 99.00 0.24 57 43 75 32 120.39 1.43 58 44 74 30 128.15 2.23 59 44 75 31 120.14 1.31 60 44 76 32 120.15 1.31 61 44 77 33 119.78 1.13 62 45 48 3 853.62 1.17 63 45 50 5 792.22 2.69 64 45 69 24 163.68 0.41 65 45 74 29 129.18 1.69 66 45 75 30 122.03 0.61 67 45 79 34 121.11 0.83 68 45 122 77 127.99 2.17 69 45 134 89 122.03 0.60 70 46 73 27 161.81 1.10 71 46 74 28 138.94 0.28 72 46 76 30 139.24 0.12 73 47 70 23 166.15 0.12 74 47 71 24 166.21 0.09 75 47 72 25 166.21 0.09 76 47 73 26 165.46 0.38 77 48 67 19 166.13 0.12 78 48 68 20 166.16 0.13 79 48 69 21 166.13 0.12 80 48 73 25 166.13 0.12 81 48 74 26 166.12 0.11 82 49 67 18 166.21 0.12 83 49 68 19 166.19 0.14 84 49 70 21 166.59 0.14 85 50 65 15 166.31 0.09 86 50 66 16 166.28 0.14 87 50 67 17 166.22 0.12 88 50 134 84 166.24 0.11 89 51 65 14 166.37 0.11 90 51 66 15 166.38 0.11 91 52 55 3 922.43 0.06 92 52 63 11 166.57 0.14 93 52 64 12 166.44 0.14 94 52 65 13 166.43 0.14 95 53 64 11 166.93 0.22 96 53 65 12 166.94 0.21 97 53 66 13 166.96 0.20 98 53 133 80 166.96 0.21 99 53 208 155 166.60 0.06 100 53 230 177 166.40 0.10 101 54 58 4 793.64 0.01 102 54 60 6 755.78 3.87 103 54 62 8 568.36 85.36 104 54 63 9 166.94 0.23 105 54 64 10 166.96 0.21 106 54 230 176 166.85 0.13 107 55 58 3 830.98 2.37 108 55 59 4 804.39 4.00 109 55 60 5 765.38 0.90 110 55 62 7 744.57 2.74 111 55 63 8 166.97 0.22 112 56 59 3 814.88 1.25 113 56 60 4 786.54 3.51 114 56 62 6 753.16 5.23 115 56 63 7 752.75 5.44 116 58 232 174 167.09 0.15 117 59 232 173 167.28 0.21 118 60 204 144 167.04 0.20 119 60 232 172 167.32 0.23 120 61 137 76 167.25 0.22 121 61 204 143 167.25 0.23 122 62 135 73 167.64 0.24 123 62 136 74 167.53 0.24 124 62 137 75 167.25 0.22 125 62 204 142 167.51 0.26 126 62 205 143 167.63 0.24 127 62 206 144 167.63 0.25 128 62 230 168 169.69 0.41 129 62 232 170 167.64 0.24 130 63 135 72 167.66 0.25 131 63 136 73 167.63 0.24 132 64 133 69 169.57 0.46 133 64 134 70 169.35 0.44 134 64 135 71 167.66 0.25 135 64 206 142 168.40 0.61 136 64 230 166 169.69 0.41 137 65 79 14 385.68 2.50 138 65 133 68 169.62 0.45 139 65 134 69 169.44 0.43 140 65 136 71 279.68 54.69 141 66 131 65 385.74 2.50 142 66 132 66 385.61 2.49 143 66 208 142 169.77 0.45 144 67 131 64 385.83 2.55 145 67 132 65 385.67 2.50 146 67 134 67 385.68 2.50 147 68 129 61 388.42 2.56 148 68 130 62 387.09 3.11 149 68 131 63 386.62 2.93 150 69 73 4 750.32 0.56 151 69 74 5 602.07 93.86 152 69 122 53 393.03 1.13 153 69 124 55 392.19 0.71 154 69 129 60 388.43 2.56 155 69 130 61 388.31 2.52 156 69 132 63 388.30 2.52 157 70 73 3 809.80 0.89 158 70 124 54 392.33 0.70 159 70 129 59 388.41 2.54 160 71 127 56 394.46 1.77 161 73 123 50 418.14 2.98 162 73 124 51 406.62 2.73 163 74 122 48 421.35 1.39 164 74 123 49 421.35 1.39 165 75 78 3 884.14 9.29 166 75 79 4 824.76 1.00 167 75 121 46 509.86 24.41 168 75 122 47 497.97 30.28 169 76 121 45 567.24 3.31 170 77 121 44 568.87 2.67 171 78 119 41 568.89 2.67 172 78 120 42 568.88 2.67 173 78 121 43 568.87 2.67 174 79 86 7 569.73 2.88 175 79 120 41 569.20 2.83 176 79 134 55 569.71 2.89 177 79 136 57 570.06 2.76 178 80 84 4 783.85 5.16 179 80 86 6 569.75 2.89 180 80 119 39 575.56 0.02 181 80 120 40 570.04 2.75 182 81 84 3 824.59 1.70 183 81 116 35 684.58 1.27 184 81 118 37 617.64 20.81 185 81 119 38 575.62 0.02 186 82 116 34 733.83 0.50 187 82 119 37 705.25 0.65 188 82 138 56 658.00 0.48 189 83 116 33 733.85 0.51 190 83 138 55 657.99 0.48 191 83 139 56 657.87 0.48 192 84 116 32 734.12 0.62 193 84 136 52 658.14 0.49 194 84 137 53 658.04 0.48 195 84 138 54 658.01 0.48 196 85 113 28 734.68 0.48 197 85 114 29 734.57 0.49 198 85 115 30 734.46 0.46 199 85 116 31 734.19 0.59 200 85 136 51 658.16 0.48 201 85 137 52 658.13 0.49 202 85 139 54 658.02 0.48 203 85 181 96 542.98 0.43 204 85 182 97 542.59 0.35 205 86 113 27 734.69 0.48 206 86 114 28 734.59 0.48 207 86 120 34 733.92 0.53 208 86 135 49 658.27 0.51 209 86 136 50 658.17 0.48 210 87 111 24 735.52 0.39 211 87 112 25 734.96 0.56 212 87 113 26 734.70 0.48 213 87 120 33 734.53 0.49 214 87 134 47 658.39 0.49 215 87 135 48 658.35 0.50 216 87 173 86 542.70 0.46 217 87 174 87 543.50 0.64 218 87 184 97 542.00 0.49 219 88 95 7 739.20 0.77 220 88 97 9 736.60 0.53 221 88 106 18 736.91 0.60 222 88 111 23 735.57 0.38 223 88 112 24 735.49 0.41 224 88 120 32 734.61 0.51 225 88 122 34 734.25 0.57 226 88 132 44 659.14 0.32 227 88 133 45 658.85 0.39 228 88 134 46 658.39 0.49 229 89 109 20 736.93 0.60 230 89 110 21 736.59 0.74 231 89 111 22 735.57 0.38 232 89 132 43 659.15 0.32 233 89 133 44 659.06 0.35 234 89 154 65 657.36 0.45 235 89 173 84 543.06 0.42 236 89 253 164 253.31 4.27 237 90 93 3 879.73 0.74 238 90 95 5 758.75 4.96 239 90 106 16 737.43 0.80 240 90 107 17 737.56 0.75 241 90 108 18 737.54 0.74 242 90 109 19 737.43 0.80 243 90 110 20 736.83 0.63 244 90 131 41 659.65 0.45 245 90 132 42 659.47 0.48 246 91 108 17 737.65 0.72 247 91 131 40 659.83 0.43 248 91 132 41 659.67 0.47 249 91 133 42 659.65 0.46 250 91 210 119 253.91 4.49 251 91 253 162 253.41 4.29 252 92 108 16 737.70 0.72 253 92 130 38 659.90 0.40 254 92 131 39 659.86 0.42 255 93 107 14 737.96 0.82 256 93 108 15 737.88 0.81 257 93 126 33 733.96 0.46 258 93 130 37 659.89 0.41 259 94 106 12 738.00 0.83 260 94 107 13 737.96 0.82 261 94 124 30 734.28 0.54 262 94 125 31 733.96 0.45 263 94 126 32 733.92 0.47 264 94 130 36 659.90 0.40 265 95 105 10 738.25 0.81 266 95 106 11 738.12 0.76 267 95 122 27 734.72 0.55 268 95 123 28 734.59 0.57 269 95 124 29 734.20 0.49 270 95 125 30 734.00 0.43 271 95 132 37 659.63 0.45 272 96 103 7 738.66 0.95 273 96 104 8 738.53 0.93 274 96 105 9 738.25 0.80 275 96 122 26 734.72 0.55 276 96 123 27 734.61 0.56 277 96 125 29 734.02 0.42 278 97 103 6 738.67 0.95 279 97 104 7 738.58 0.93 280 97 106 9 738.55 0.94 281 97 112 15 737.99 0.84 282 97 121 24 734.84 0.53 283 97 122 25 734.66 0.58 284 98 102 4 782.27 7.05 285 98 103 5 738.66 0.96 286 98 114 16 737.98 0.85 287 98 120 22 735.22 0.59 288 98 121 23 735.00 0.51 289 99 102 3 846.53 2.82 290 99 114 15 737.98 0.84 291 99 118 19 735.42 0.56 292 99 119 20 735.38 0.55 293 99 121 22 735.23 0.59 294 99 149 50 657.66 0.45 295 100 121 21 735.34 0.55 296 102 149 47 657.83 0.48 297 104 149 45 657.87 0.49 298 104 151 47 657.55 0.44 299 105 125 20 734.02 0.44 300 106 110 4 775.13 7.90 301 106 111 5 774.02 8.46 302 106 112 6 753.06 2.03 303 106 151 45 657.60 0.46 304 107 110 3 814.08 0.53 305 108 155 47 657.36 0.43 306 109 153 44 657.40 0.44 307 109 154 45 657.35 0.45 308 110 152 42 657.46 0.46 309 110 153 43 657.37 0.45 310 111 150 39 657.52 0.46 311 111 151 40 657.45 0.47 312 111 152 41 657.39 0.46 313 111 153 42 657.37 0.45 314 111 154 43 657.37 0.45 315 111 157 46 625.59 2.58 316 111 173 62 543.25 0.62 317 111 174 63 544.24 0.42 318 112 120 8 734.74 0.48 319 112 150 38 657.50 0.46 320 112 151 39 657.40 0.46 321 113 147 34 657.72 0.45 322 113 148 35 657.65 0.47 323 113 149 36 657.49 0.46 324 113 150 37 657.37 0.46 325 113 174 61 542.50 0.41 326 113 180 67 544.42 0.39 327 113 182 69 544.08 0.48 328 114 118 4 804.23 8.13 329 114 119 5 793.94 13.27 330 114 120 6 734.88 0.47 331 114 149 35 657.49 0.46 332 115 118 3 832.51 3.16 333 115 147 32 657.62 0.48 334 115 148 33 657.56 0.48 335 115 149 34 657.49 0.46 336 116 139 23 658.00 0.48 337 116 147 31 657.67 0.46 338 118 149 31 657.56 0.42 339 122 134 12 658.69 0.55 340 124 128 4 849.06 5.58 341 124 129 5 791.37 5.66 342 124 130 6 662.72 1.94 343 126 129 3 926.66 6.55 344 126 130 4 835.01 0.40 345 133 184 51 543.25 0.33 346 133 208 75 253.38 4.32 347 133 230 97 253.37 4.32 348 133 253 120 253.38 4.31 349 135 182 47 543.81 0.57 350 135 184 49 541.00 1.17 351 135 255 120 243.47 0.13 352 137 181 44 542.85 0.46 353 137 182 45 542.34 0.37 354 137 257 120 241.47 0.79 355 139 146 7 657.61 0.46 356 139 147 8 657.59 0.47 357 139 179 40 544.23 0.46 358 139 181 42 542.56 0.38 359 139 260 121 240.72 0.71 360 140 181 41 542.75 0.47 361 140 260 120 240.72 0.71 362 140 261 121 239.88 0.29 363 142 145 3 828.72 2.55 364 142 178 36 544.36 0.40 365 142 260 118 240.76 0.71 366 145 178 33 544.41 0.39 367 145 179 34 544.40 0.38 368 146 179 33 544.39 0.38 369 147 179 32 544.35 0.36 370 147 180 33 544.24 0.35 371 148 175 27 544.31 0.39 372 148 179 31 544.40 0.39 373 148 180 32 544.36 0.41 374 150 173 23 543.02 0.12 375 150 174 24 543.79 0.33 376 150 175 25 544.31 0.39 377 150 180 30 544.50 0.41 378 153 156 3 836.38 2.84 379 153 157 4 831.61 4.24 380 154 157 3 828.49 1.14 381 154 171 17 543.97 0.42 382 154 173 19 544.34 0.42 383 154 186 32 543.62 0.47 384 157 171 14 545.04 0.28 385 157 172 15 545.04 0.28 386 159 169 10 545.14 0.23 387 159 170 11 545.12 0.24 388 159 171 12 545.04 0.28 389 160 169 9 545.20 0.25 390 160 170 10 545.18 0.25 391 160 176 16 545.33 0.32 392 161 164 3 828.91 0.71 393 161 165 4 759.75 3.91 394 161 166 5 546.63 0.06 395 161 168 7 546.23 0.27 396 161 169 8 545.70 0.01 397 162 165 3 835.45 2.56 398 162 168 6 546.63 0.06 399 162 170 8 545.73 0.03 400 162 187 25 545.11 0.33 401 162 195 33 477.25 0.09 402 162 199 37 476.40 0.40 403 163 195 32 477.33 0.06 404 163 196 33 476.51 0.38 405 163 197 34 476.51 0.38 406 165 168 3 842.64 1.04 407 165 194 29 517.95 3.50 408 165 195 30 491.74 2.58 409 165 196 31 477.22 0.07 410 167 189 22 544.95 0.33 411 167 190 23 544.70 0.43 412 167 193 26 502.80 0.91 413 168 187 19 545.12 0.33 414 168 188 20 544.96 0.36 415 168 189 21 544.70 0.43 416 168 193 25 488.73 4.09 417 168 194 26 478.92 0.68 418 169 187 18 545.01 0.39 419 169 188 19 544.96 0.37 420 170 185 15 545.32 0.43 421 170 186 16 545.23 0.39 422 170 187 17 545.00 0.42 423 170 254 84 254.97 4.00 424 171 185 14 545.32 0.43 425 171 186 15 545.25 0.41 426 171 188 17 545.23 0.41 427 172 176 4 828.83 0.63 428 172 184 12 545.46 0.38 429 172 185 13 545.32 0.43 430 173 183 10 545.59 0.35 431 173 184 11 545.46 0.37 432 173 186 13 545.44 0.37 433 174 180 6 644.34 48.62 434 174 182 8 545.81 0.33 435 174 183 9 545.58 0.36 436 175 180 5 750.08 1.90 437 177 180 3 837.50 0.83 438 177 259 82 241.25 0.66 439 178 259 81 240.88 0.70 440 178 260 82 240.88 0.70 441 180 259 79 240.88 0.71 442 180 260 80 240.88 0.70 443 181 257 76 241.63 0.76 444 181 258 77 241.46 0.78 445 181 260 79 240.80 0.70 446 182 256 74 243.47 0.13 447 182 257 75 241.57 0.73 448 182 258 76 240.88 0.70 449 183 255 72 248.47 2.19 450 183 256 73 243.51 0.14 451 184 253 69 254.16 4.04 452 184 254 70 253.27 4.25 453 184 255 71 250.36 3.23 454 185 253 68 253.91 4.19 455 185 254 69 253.47 4.17 456 185 256 71 253.29 4.24 457 186 251 65 255.22 4.01 458 186 252 66 254.91 4.01 459 186 253 67 254.44 3.93 460 187 195 8 546.32 0.46 461 187 251 64 255.31 4.06 462 187 252 65 255.19 4.02 463 187 254 67 254.94 4.00 464 188 249 61 259.46 3.52 465 188 250 62 259.06 3.60 466 188 251 63 256.60 3.98 467 189 193 4 784.63 6.54 468 189 194 5 751.52 1.67 469 189 195 6 751.52 1.67 470 189 243 54 290.66 0.63 471 189 249 60 259.77 3.68 472 189 250 61 259.41 3.51 473 189 252 63 259.33 3.48 474 190 193 3 811.38 1.51 475 190 243 53 292.88 0.09 476 191 243 52 292.88 0.09 477 191 245 54 292.85 0.10 478 191 246 55 292.83 0.11 479 192 243 51 292.90 0.09 480 193 242 49 292.92 0.09 481 193 243 50 292.91 0.09 482 194 241 47 293.18 0.21 483 194 242 48 292.91 0.09 484 195 199 4 850.99 0.91 485 195 240 45 293.49 0.21 486 195 241 46 293.38 0.26 487 195 252 57 292.21 0.41 488 197 240 43 299.98 1.59 489 198 217 19 300.47 1.46 490 198 220 22 301.81 0.96 491 198 238 40 300.78 1.52 492 198 239 41 300.47 1.45 493 198 240 42 300.00 1.60 494 199 237 38 318.06 0.48 495 199 238 39 302.64 0.59 496 199 239 40 300.48 1.46 497 200 236 36 324.35 3.62 498 201 205 4 929.62 2.21 499 201 235 34 348.55 0.77 500 201 236 35 345.78 1.93 501 201 239 38 333.74 2.56 502 202 235 33 348.58 0.75 503 202 236 34 347.81 1.17 504 202 262 60 254.14 3.97 505 203 235 32 348.62 0.77 506 203 256 53 291.75 0.43 507 203 257 54 286.86 0.82 508 204 233 29 349.05 0.97 509 204 234 30 349.05 0.97 510 204 235 31 348.62 0.77 511 204 255 51 293.28 0.27 512 204 256 52 291.08 0.76 513 204 257 53 291.08 0.76 514 205 232 27 355.03 0.28 515 205 233 28 349.06 0.97 516 205 239 34 349.06 0.96 517 205 254 49 293.35 0.30 518 205 255 50 292.98 0.38 519 205 256 51 292.01 0.56 520 206 230 24 359.78 0.39 521 206 231 25 356.87 1.04 522 206 254 48 295.54 1.40 523 206 255 49 293.28 0.28 524 207 230 23 359.85 0.41 525 207 231 24 358.35 0.31 526 207 233 26 358.83 0.07 527 207 252 45 300.36 0.72 528 207 253 46 299.12 0.59 529 207 254 47 293.36 0.31 530 208 228 20 364.45 0.07 531 208 229 21 363.62 0.07 532 208 230 22 360.75 0.09 533 208 252 44 301.49 0.16 534 208 253 45 299.13 0.59 535 209 225 16 365.07 0.29 536 209 226 17 365.62 0.58 537 209 227 18 364.68 0.12 538 209 228 19 364.47 0.07 539 209 229 20 364.30 0.07 540 209 231 22 362.28 0.35 541 209 250 41 303.78 0.47 542 209 251 42 303.65 0.45 543 209 252 43 301.48 0.16 544 210 227 17 365.71 0.62 545 210 251 41 303.75 0.47 546 210 252 42 302.39 0.03 547 210 253 43 303.48 0.52 548 211 227 16 367.68 0.58 549 211 248 37 308.11 0.45 550 211 249 38 306.58 0.23 551 211 250 39 304.45 0.80 552 211 251 40 303.73 0.48 553 212 226 14 367.82 0.52 554 212 227 15 367.80 0.53 555 212 248 36 308.10 0.44 556 212 249 37 306.64 0.26 557 212 250 38 304.42 0.80 558 213 225 12 370.98 0.38 559 213 226 13 367.81 0.53 560 213 243 30 349.33 1.00 561 213 244 31 349.27 1.03 562 213 245 32 348.84 0.84 563 213 250 37 304.68 0.94 564 214 224 10 373.63 0.35 565 214 225 11 371.35 0.57 566 214 242 28 354.54 1.51 567 214 243 29 349.34 1.01 568 214 244 30 349.30 1.02 569 214 250 36 303.71 0.46 570 215 222 7 475.46 36.37 571 215 223 8 379.51 3.08 572 215 224 9 373.64 0.35 573 215 241 26 359.85 0.35 574 215 242 27 357.12 0.21 575 215 244 29 352.76 0.69 576 216 221 5 784.63 0.77 577 216 222 6 475.72 36.25 578 216 223 7 403.10 0.20 579 216 225 9 373.92 0.39 580 216 231 15 368.57 0.85 581 216 233 17 367.84 0.52 582 216 239 23 360.15 0.32 583 216 240 24 360.09 0.33 584 216 241 25 359.16 0.43 585 216 252 36 303.17 0.67 586 217 220 3 822.23 5.44 587 217 221 4 778.04 2.53 588 217 222 5 548.33 0.03 589 217 239 22 360.15 0.32 590 217 240 23 360.12 0.32 591 218 221 3 814.85 0.57 592 218 223 5 779.07 3.54 593 218 231 13 369.98 0.23 594 218 233 15 367.79 0.53 595 218 238 20 360.40 0.24 596 218 239 21 360.35 0.26 597 219 238 19 360.43 0.23 598 220 238 18 360.44 0.23 599 222 241 19 360.12 0.32 600 222 242 20 360.11 0.32 601 225 229 4 795.37 2.72 602 225 231 6 659.77 46.12 603 226 229 3 846.63 1.67 604 227 248 21 319.80 6.29 605 233 237 4 792.81 11.54 606 233 239 6 408.84 0.91 607 234 237 3 834.28 2.99 608 243 250 7 303.24 0.62