BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1063 1127 64 394.04 1.05 2 1063 1136 73 406.84 2.49 3 1064 1067 3 651.68 1.76 4 1064 1068 4 634.51 1.13 5 1065 1068 3 643.51 2.33 6 1065 1069 4 636.13 1.36 7 1066 1069 3 642.01 2.10 8 1066 1070 4 634.59 1.10 9 1066 1120 54 402.19 5.12 10 1066 1123 57 407.42 2.50 11 1066 1124 58 406.92 2.75 12 1066 1127 61 408.25 2.09 13 1066 1139 73 408.25 2.10 14 1066 1143 77 407.34 2.55 15 1067 1070 3 644.41 3.81 16 1067 1071 4 634.59 1.10 17 1067 1139 72 408.26 2.10 18 1067 1142 75 407.69 2.39 19 1068 1071 3 644.41 3.81 20 1068 1072 4 624.95 3.71 21 1069 1072 3 647.54 7.13 22 1069 1073 4 628.59 1.89 23 1069 1120 51 412.47 0.09 24 1070 1073 3 645.32 0.54 25 1070 1074 4 627.32 2.52 26 1070 1120 50 412.49 0.10 27 1070 1139 69 412.98 0.15 28 1070 1142 72 412.67 0.01 29 1070 1143 73 412.36 0.04 30 1070 1146 76 408.19 2.11 31 1071 1074 3 651.19 2.04 32 1071 1075 4 632.23 0.06 33 1071 1142 71 412.98 0.17 34 1072 1075 3 644.76 5.21 35 1072 1076 4 632.22 0.05 36 1073 1076 3 644.04 1.48 37 1073 1077 4 635.75 2.68 38 1073 1113 40 413.10 0.12 39 1073 1116 43 413.04 0.14 40 1073 1117 44 413.04 0.15 41 1073 1146 73 412.89 0.24 42 1073 1150 77 412.50 0.42 43 1074 1077 3 635.76 2.67 44 1074 1078 4 635.75 2.68 45 1074 1142 68 413.16 0.24 46 1074 1146 72 412.90 0.23 47 1075 1078 3 646.20 2.55 48 1075 1079 4 646.14 2.52 49 1076 1079 3 646.14 2.53 50 1076 1080 4 646.13 2.54 51 1076 1109 33 413.69 0.04 52 1076 1110 34 413.64 0.04 53 1076 1113 37 413.39 0.18 54 1076 1150 74 412.67 0.46 55 1076 1153 77 409.64 1.39 56 1077 1080 3 646.54 2.33 57 1077 1081 4 634.02 0.92 58 1077 1146 69 413.19 0.25 59 1077 1149 72 412.73 0.44 60 1077 1150 73 412.67 0.46 61 1077 1153 76 409.59 1.40 62 1078 1081 3 654.94 1.87 63 1078 1082 4 634.02 0.92 64 1079 1082 3 645.48 0.96 65 1079 1083 4 634.00 0.93 66 1080 1083 3 648.94 6.42 67 1080 1084 4 633.59 0.72 68 1080 1106 26 414.13 0.16 69 1080 1110 30 413.70 0.04 70 1080 1153 73 411.87 0.29 71 1081 1084 3 638.60 1.42 72 1081 1085 4 630.85 1.98 73 1081 1149 68 412.80 0.45 74 1081 1152 71 412.60 0.46 75 1081 1153 72 409.64 1.38 76 1081 1156 75 409.13 1.34 77 1082 1085 3 636.37 2.94 78 1082 1086 4 630.94 2.02 79 1083 1086 3 644.10 0.93 80 1083 1106 23 414.28 0.14 81 1084 1103 19 414.41 0.19 82 1084 1106 22 414.29 0.14 83 1084 1153 69 412.49 0.41 84 1084 1156 72 409.23 1.30 85 1084 1157 73 409.09 1.34 86 1084 1160 76 381.87 2.91 87 1086 1160 74 403.78 2.16 88 1088 1099 11 415.82 0.19 89 1088 1160 72 409.23 1.31 90 1089 1160 71 409.30 1.29 91 1089 1163 74 394.12 0.71 92 1089 1167 78 368.56 0.05 93 1089 1240 151 100.39 1.33 94 1090 1095 5 519.58 31.85 95 1090 1096 6 417.04 0.18 96 1090 1099 9 417.03 0.17 97 1090 1164 74 382.35 2.69 98 1090 1167 77 368.56 0.05 99 1090 1171 81 358.60 2.32 100 1091 1095 4 627.65 2.54 101 1091 1171 80 358.95 2.44 102 1092 1095 3 635.87 1.56 103 1092 1096 4 617.25 2.67 104 1092 1171 79 358.94 2.43 105 1093 1096 3 633.70 0.48 106 1093 1097 4 630.10 2.27 107 1093 1168 75 368.80 0.06 108 1093 1171 78 358.65 2.31 109 1093 1172 79 358.45 2.41 110 1093 1175 82 328.82 1.77 111 1094 1097 3 643.19 2.01 112 1094 1098 4 624.09 0.74 113 1095 1098 3 638.13 0.58 114 1095 1099 4 631.49 1.07 115 1096 1099 3 644.16 0.18 116 1096 1100 4 633.64 0.01 117 1096 1168 72 360.83 1.69 118 1096 1171 75 358.44 2.39 119 1097 1100 3 648.34 1.62 120 1097 1101 4 633.75 0.06 121 1097 1168 71 367.18 0.32 122 1098 1101 3 645.92 3.14 123 1098 1102 4 633.36 0.26 124 1099 1102 3 638.73 2.44 125 1099 1103 4 638.72 2.43 126 1099 1160 61 408.80 1.51 127 1099 1164 65 373.01 1.28 128 1100 1103 3 644.88 0.64 129 1100 1104 4 638.72 2.44 130 1100 1164 64 369.53 0.32 131 1100 1165 65 369.52 0.32 132 1100 1168 68 359.16 2.50 133 1101 1104 3 638.72 2.45 134 1101 1105 4 638.72 2.46 135 1102 1105 3 640.62 3.43 136 1102 1106 4 638.70 2.46 137 1103 1106 3 644.61 2.12 138 1103 1107 4 636.68 1.75 139 1103 1157 54 409.31 1.43 140 1103 1160 57 403.92 2.15 141 1103 1161 58 384.59 1.55 142 1103 1164 61 369.55 0.30 143 1104 1107 3 641.89 0.86 144 1104 1108 4 634.97 1.65 145 1105 1108 3 655.66 1.95 146 1105 1109 4 632.49 0.41 147 1106 1109 3 639.20 0.48 148 1106 1110 4 627.99 2.66 149 1106 1157 51 409.13 1.34 150 1107 1110 3 647.38 3.30 151 1107 1111 4 626.68 2.00 152 1107 1157 50 407.63 1.77 153 1108 1111 3 647.38 3.29 154 1108 1112 4 627.26 1.70 155 1109 1112 3 647.14 3.58 156 1109 1113 4 636.19 1.90 157 1110 1113 3 645.67 3.54 158 1110 1114 4 634.62 1.99 159 1110 1150 40 412.94 0.51 160 1110 1153 43 412.19 0.21 161 1110 1157 47 407.31 1.74 162 1111 1114 3 640.16 0.79 163 1113 1116 3 647.64 3.02 164 1113 1117 4 618.98 1.98 165 1113 1150 37 413.05 0.56 166 1114 1117 3 639.98 0.82 167 1114 1118 4 636.33 2.64 168 1114 1150 36 413.06 0.56 169 1115 1118 3 646.27 0.90 170 1115 1119 4 640.94 0.33 171 1116 1119 3 651.98 5.19 172 1116 1120 4 631.84 0.40 173 1117 1120 3 656.12 3.11 174 1117 1121 4 626.68 2.99 175 1117 1146 29 413.74 0.43 176 1117 1147 30 413.73 0.43 177 1117 1150 33 413.11 0.70 178 1118 1121 3 632.26 4.61 179 1118 1122 4 626.42 3.12 180 1119 1122 3 640.20 0.65 181 1119 1123 4 631.12 5.20 182 1120 1123 3 654.36 6.41 183 1120 1124 4 635.66 2.93 184 1120 1143 23 413.99 0.46 185 1120 1146 26 413.89 0.42 186 1121 1124 3 644.80 1.62 187 1121 1125 4 653.85 1.08 188 1121 1147 26 413.80 0.40 189 1122 1125 3 653.86 1.08 190 1122 1126 4 653.85 1.08 191 1123 1126 3 662.46 3.21 192 1123 1127 4 640.89 0.26 193 1124 1127 3 649.43 3.30 194 1124 1128 4 640.88 0.27 195 1124 1143 19 414.06 0.45 196 1125 1128 3 657.02 1.32 197 1125 1129 4 632.14 2.06 198 1125 1143 18 414.18 0.45 199 1126 1129 3 637.27 3.81 200 1127 1131 4 648.54 0.61 201 1127 1136 9 415.06 0.56 202 1127 1139 12 414.88 0.58 203 1128 1131 3 658.65 4.44 204 1128 1136 8 414.88 0.58 205 1128 1139 11 414.88 0.58 206 1128 1140 12 414.86 0.58 207 1128 1143 15 414.71 0.51 208 1131 1136 5 561.46 26.83 209 1132 1137 5 608.90 3.11 210 1133 1136 3 650.26 5.70 211 1133 1137 4 628.42 0.21 212 1134 1137 3 650.12 4.87 213 1134 1138 4 628.43 0.20 214 1135 1138 3 635.83 3.18 215 1135 1139 4 627.79 0.38 216 1136 1139 3 632.81 2.89 217 1136 1140 4 621.86 3.34 218 1137 1140 3 635.63 2.71 219 1137 1141 4 632.66 2.14 220 1138 1141 3 638.84 2.93 221 1138 1142 4 633.84 1.55 222 1139 1142 3 641.26 0.71 223 1139 1143 4 645.43 7.34 224 1140 1143 3 663.30 1.58 225 1140 1144 4 659.48 0.33 226 1141 1144 3 659.48 0.33 227 1141 1145 4 659.49 0.34 228 1142 1145 3 659.49 0.34 229 1142 1146 4 644.90 7.63 230 1143 1146 3 659.48 0.34 231 1143 1147 4 644.88 7.63 232 1144 1147 3 651.31 4.42 233 1144 1148 4 634.18 2.30 234 1145 1148 3 649.02 3.74 235 1145 1149 4 634.11 2.29 236 1146 1149 3 655.48 0.20 237 1146 1150 4 632.84 2.92 238 1147 1150 3 647.04 2.54 239 1147 1151 4 632.93 2.85 240 1148 1151 3 654.46 5.52 241 1148 1152 4 630.61 4.00 242 1149 1152 3 640.05 0.74 243 1149 1153 4 630.83 2.91 244 1150 1153 3 657.22 4.15 245 1150 1154 4 639.33 1.31 246 1151 1154 3 644.89 3.64 247 1151 1155 4 640.53 0.71 248 1152 1155 3 649.90 0.49 249 1152 1156 4 640.52 0.70 250 1153 1156 3 651.42 0.27 251 1153 1157 4 640.54 0.69 252 1154 1157 3 651.53 0.21 253 1154 1158 4 640.59 0.66 254 1155 1158 3 651.08 0.22 255 1155 1159 4 640.56 0.64 256 1156 1159 3 651.08 0.22 257 1156 1160 4 640.54 0.65 258 1157 1160 3 637.68 0.93 259 1157 1161 4 634.60 2.41 260 1158 1161 3 645.32 7.26 261 1158 1162 4 634.59 2.40 262 1159 1162 3 644.81 7.52 263 1159 1163 4 636.64 3.43 264 1160 1163 3 646.23 4.11 265 1160 1164 4 636.64 3.44 266 1161 1164 3 649.39 2.52 267 1161 1165 4 634.02 2.67 268 1162 1165 3 652.81 0.82 269 1162 1166 4 636.65 3.45 270 1163 1166 3 657.58 7.44 271 1163 1167 4 634.60 4.48 272 1163 1240 77 100.03 1.33 273 1164 1167 3 634.35 4.60 274 1164 1168 4 632.10 3.48 275 1165 1168 3 634.86 2.10 276 1165 1169 4 632.03 3.51 277 1166 1169 3 631.62 3.71 278 1166 1170 4 630.64 4.21 279 1166 1239 73 100.07 1.35 280 1166 1240 74 100.23 1.30 281 1167 1170 3 630.82 4.12 282 1167 1171 4 630.57 4.17 283 1167 1241 74 100.25 1.31 284 1168 1171 3 643.76 2.44 285 1168 1172 4 625.51 1.80 286 1169 1172 3 645.97 3.33 287 1169 1173 4 651.88 0.37 288 1169 1234 65 100.35 1.36 289 1170 1173 3 649.68 1.48 290 1170 1174 4 639.73 6.45 291 1170 1234 64 100.45 1.34 292 1170 1241 71 100.36 1.35 293 1170 1244 74 100.36 1.33 294 1170 1245 75 100.19 1.39 295 1171 1174 3 644.97 3.83 296 1171 1175 4 639.73 6.45 297 1172 1175 3 646.95 3.62 298 1172 1176 4 627.23 0.90 299 1173 1176 3 650.74 0.68 300 1173 1177 4 630.47 3.06 301 1173 1227 54 100.70 1.35 302 1173 1230 57 100.66 1.35 303 1173 1231 58 100.59 1.39 304 1173 1234 61 100.62 1.37 305 1173 1245 72 100.41 1.34 306 1173 1248 75 99.03 1.29 307 1173 1249 76 98.84 1.35 308 1174 1177 3 644.53 2.42 309 1174 1178 4 631.99 3.85 310 1174 1248 74 99.46 1.06 311 1175 1178 3 646.87 0.61 312 1175 1179 4 631.99 3.85 313 1176 1179 3 642.68 1.49 314 1176 1180 4 630.47 3.08 315 1176 1227 51 100.75 1.35 316 1177 1180 3 650.16 2.26 317 1177 1181 4 641.08 2.27 318 1177 1227 50 100.77 1.34 319 1177 1248 71 99.33 1.28 320 1177 1252 75 98.25 1.57 321 1178 1181 3 638.58 0.32 322 1178 1182 4 631.75 3.73 323 1179 1182 3 645.71 1.26 324 1179 1183 4 635.50 5.63 325 1179 1223 44 101.05 1.29 326 1180 1183 3 644.34 3.25 327 1180 1184 4 631.66 3.89 328 1180 1223 43 101.05 1.30 329 1180 1224 44 101.00 1.30 330 1180 1227 47 100.93 1.34 331 1180 1252 72 98.44 1.52 332 1181 1184 3 637.46 4.66 333 1181 1185 4 635.33 5.72 334 1181 1248 67 100.44 1.35 335 1181 1252 71 98.44 1.50 336 1181 1255 74 97.73 1.59 337 1182 1185 3 641.78 2.50 338 1182 1186 4 635.33 5.73 339 1183 1186 3 645.18 2.61 340 1183 1187 4 624.71 0.33 341 1183 1220 37 101.89 1.22 342 1183 1223 40 101.77 1.22 343 1183 1224 41 101.78 1.22 344 1184 1187 3 639.56 0.20 345 1184 1188 4 622.21 0.92 346 1184 1224 40 101.80 1.23 347 1184 1252 68 98.75 1.40 348 1184 1255 71 97.95 1.69 349 1184 1256 72 97.31 1.51 350 1184 1259 75 84.06 2.09 351 1185 1188 3 627.36 1.61 352 1185 1189 4 622.21 0.92 353 1185 1255 70 97.98 1.70 354 1186 1189 3 635.54 4.77 355 1186 1190 4 628.17 3.71 356 1187 1190 3 646.06 3.39 357 1187 1191 4 628.18 3.72 358 1187 1213 26 102.56 1.18 359 1187 1217 30 102.17 1.24 360 1187 1259 72 87.67 0.42 361 1188 1191 3 646.32 2.88 362 1188 1192 4 628.16 3.71 363 1188 1255 67 98.48 1.53 364 1188 1259 71 89.99 2.12 365 1189 1192 3 643.57 2.06 366 1190 1193 3 689.41 4.03 367 1190 1213 23 103.00 1.30 368 1191 1210 19 103.09 1.32 369 1191 1213 22 103.05 1.31 370 1191 1259 68 96.78 1.76 371 1191 1262 71 83.76 2.08 372 1191 1263 72 81.08 2.56 373 1191 1266 75 49.98 0.01 374 1192 1262 70 88.06 0.80 375 1194 1266 72 71.78 1.00 376 1194 1269 75 44.41 1.38 377 1194 1270 76 41.90 0.89 378 1197 1202 5 336.49 86.30 379 1197 1203 6 139.40 1.43 380 1198 1202 4 605.45 0.07 381 1199 1202 3 637.37 2.71 382 1199 1203 4 636.60 3.10 383 1200 1203 3 636.60 3.10 384 1200 1204 4 623.33 3.54 385 1201 1204 3 642.90 0.06 386 1201 1205 4 636.59 3.10 387 1202 1205 3 632.76 0.74 388 1202 1206 4 632.32 0.96 389 1203 1206 3 651.52 8.64 390 1203 1207 4 631.56 1.34 391 1204 1207 3 655.65 4.74 392 1204 1208 4 631.82 1.23 393 1205 1208 3 631.83 1.23 394 1205 1209 4 625.57 4.36 395 1206 1209 3 638.13 4.00 396 1206 1210 4 631.16 2.14 397 1207 1210 3 645.69 2.89 398 1207 1211 4 632.78 1.33 399 1207 1270 63 42.46 0.59 400 1208 1211 3 632.72 0.79 401 1208 1212 4 631.59 0.73 402 1209 1212 3 645.66 0.23 403 1209 1213 4 622.85 5.64 404 1210 1213 3 623.00 5.57 405 1210 1214 4 632.09 1.03 406 1210 1263 53 83.48 1.80 407 1210 1266 56 55.97 1.95 408 1210 1267 57 51.39 0.35 409 1210 1270 60 39.09 2.11 410 1211 1214 3 644.40 1.40 411 1211 1215 4 635.74 0.80 412 1212 1215 3 636.79 0.27 413 1212 1216 4 636.80 0.28 414 1213 1216 3 637.05 0.15 415 1213 1217 4 640.79 2.27 416 1214 1217 3 646.73 0.44 417 1214 1218 4 640.82 2.27 418 1214 1263 49 79.59 2.92 419 1214 1267 53 46.16 2.30 420 1215 1218 3 642.82 3.26 421 1215 1219 4 640.82 2.26 422 1216 1219 3 641.90 1.73 423 1216 1220 4 640.82 2.26 424 1217 1220 3 644.73 3.14 425 1217 1221 4 632.15 2.08 426 1217 1259 42 92.36 2.08 427 1217 1260 43 83.93 1.99 428 1217 1263 46 70.66 0.74 429 1218 1221 3 642.28 2.56 430 1220 1223 3 632.63 2.15 431 1220 1224 4 646.10 0.01 432 1221 1224 3 646.11 0.01 433 1221 1225 4 646.12 0.03 434 1221 1256 35 97.21 1.48 435 1222 1225 3 646.31 0.12 436 1222 1226 4 646.12 0.03 437 1223 1226 3 649.73 1.73 438 1223 1227 4 649.73 1.73 439 1224 1227 3 678.10 1.86 440 1224 1252 28 98.45 1.50 441 1224 1256 32 93.05 2.43 442 1225 1228 3 649.73 1.74 443 1225 1229 4 647.43 2.89 444 1225 1256 31 93.14 2.39 445 1226 1229 3 651.37 0.46 446 1226 1230 4 634.43 6.02 447 1227 1230 3 634.96 5.43 448 1227 1231 4 622.88 2.85 449 1227 1252 25 98.27 1.56 450 1228 1231 3 641.16 3.15 451 1228 1232 4 632.32 7.57 452 1228 1249 21 99.00 1.28 453 1228 1252 24 98.27 1.56 454 1228 1253 25 98.03 1.67 455 1229 1232 3 646.61 2.06 456 1229 1233 4 632.31 7.58 457 1230 1233 3 644.20 3.27 458 1230 1234 4 632.80 7.19 459 1231 1234 3 641.62 2.78 460 1231 1235 4 632.83 7.17 461 1231 1245 14 109.36 3.12 462 1231 1249 18 98.84 1.35 463 1232 1235 3 641.61 2.77 464 1232 1236 4 610.56 3.98 465 1232 1249 17 106.83 2.63 466 1233 1236 3 625.75 2.29 467 1233 1237 4 620.67 1.07 468 1234 1237 3 641.67 2.62 469 1234 1238 4 620.67 1.07 470 1234 1239 5 371.56 78.64 471 1234 1245 11 123.48 0.04 472 1235 1238 3 643.60 3.58 473 1235 1242 7 126.48 1.46 474 1235 1245 10 123.49 0.04 475 1235 1246 11 120.52 1.45 476 1239 1242 3 641.15 3.08 477 1239 1245 6 606.20 2.79 478 1241 1244 3 653.50 2.25 479 1241 1245 4 641.08 3.15 480 1242 1245 3 641.09 3.15 481 1242 1246 4 622.39 6.21 482 1243 1246 3 641.62 2.89 483 1243 1247 4 643.00 4.09 484 1244 1247 3 651.83 2.10 485 1244 1248 4 644.30 3.44 486 1245 1248 3 649.41 0.89 487 1245 1249 4 632.65 2.38 488 1246 1249 3 649.24 0.80 489 1246 1250 4 642.35 2.46 490 1247 1250 3 650.22 0.31 491 1247 1251 4 637.46 0.02 492 1248 1251 3 642.37 2.46 493 1248 1252 4 637.41 0.03 494 1249 1252 3 648.51 0.76 495 1249 1253 4 637.38 0.04 496 1250 1253 3 645.16 3.93 497 1250 1254 4 632.62 2.33 498 1251 1254 3 644.26 2.84 499 1251 1255 4 622.72 7.29 500 1252 1255 3 646.75 0.48 501 1252 1256 4 624.89 6.20 502 1253 1256 3 643.10 2.91 503 1253 1257 4 632.73 2.28 504 1254 1257 3 649.61 6.17 505 1254 1258 4 633.13 2.05 506 1255 1258 3 648.27 0.82 507 1255 1259 4 632.67 2.25 508 1256 1259 3 639.08 0.93 509 1256 1260 4 634.46 1.35 510 1257 1260 3 648.73 2.33 511 1257 1261 4 636.62 0.70 512 1258 1261 3 642.58 3.43 513 1258 1262 4 627.97 5.03 514 1259 1262 3 641.11 1.49 515 1259 1263 4 623.11 2.47 516 1260 1263 3 636.87 3.61 517 1260 1264 4 630.36 0.35 518 1261 1264 3 641.11 1.49 519 1261 1265 4 620.30 5.38 520 1262 1265 3 643.26 2.91 521 1262 1266 4 620.29 5.39 522 1263 1266 3 648.07 0.43 523 1263 1267 4 638.82 4.20 524 1264 1267 3 649.65 0.36 525 1264 1268 4 639.55 3.83 526 1265 1268 3 648.07 1.16 527 1265 1269 4 624.00 2.64 528 1266 1269 3 643.31 3.66 529 1266 1270 4 632.89 7.09 530 1267 1270 3 652.87 5.86 531 1267 1271 4 627.17 4.24 532 1268 1271 3 642.06 3.23 533 1268 1272 4 627.16 4.24 534 1269 1272 3 641.89 3.31 535 1269 1273 4 627.07 4.29