BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 5 13 8 156.44 1.19 2 6 9 3 883.55 5.02 3 6 10 4 694.63 47.36 4 6 11 5 478.90 60.51 5 6 80 74 155.74 1.31 6 6 83 77 156.43 1.19 7 6 84 78 156.44 1.19 8 7 10 3 846.78 3.21 9 7 80 73 156.47 1.21 10 8 47 39 156.46 1.23 11 8 50 42 156.41 1.21 12 8 76 68 156.09 1.32 13 8 80 72 156.58 1.25 14 9 47 38 156.79 1.29 15 9 50 41 156.43 1.22 16 9 65 56 156.41 1.21 17 9 77 68 156.37 1.23 18 9 80 71 156.79 1.34 19 9 81 72 156.58 1.25 20 9 84 75 159.99 1.40 21 10 45 35 158.38 1.11 22 10 47 37 157.83 0.83 23 11 15 4 842.88 7.68 24 11 16 5 834.25 3.44 25 11 44 33 159.67 0.93 26 11 45 34 159.11 0.95 27 11 52 41 159.08 0.95 28 11 84 73 163.65 0.84 29 12 15 3 858.09 0.09 30 12 16 4 858.09 0.08 31 12 84 72 163.69 0.84 32 13 16 3 870.60 6.33 33 13 84 71 164.28 1.09 34 13 85 72 165.06 1.43 35 14 17 3 868.68 2.97 36 15 44 29 224.43 4.95 37 15 54 39 225.03 5.04 38 16 19 3 849.59 6.55 39 16 54 38 225.81 4.65 40 16 61 45 267.43 0.35 41 16 63 47 225.81 4.65 42 16 84 68 203.36 1.80 43 16 86 70 191.68 0.17 44 17 54 37 266.95 0.30 45 17 61 44 276.32 2.25 46 19 61 42 281.93 0.12 47 19 62 43 281.05 0.10 48 19 63 44 278.97 0.94 49 19 86 67 217.05 2.70 50 20 86 66 217.05 2.70 51 20 87 67 217.05 2.70 52 20 88 68 217.03 2.69 53 20 212 192 217.06 2.69 54 21 62 41 283.26 0.53 55 21 63 42 283.28 0.53 56 21 86 65 217.14 2.69 57 21 88 67 217.03 2.69 58 21 90 69 217.00 2.69 59 21 212 191 217.09 2.69 60 21 249 228 52.66 0.53 61 21 252 231 38.95 0.01 62 21 253 232 34.87 1.03 63 22 63 41 283.27 0.53 64 22 81 59 217.38 2.73 65 22 86 64 217.27 2.71 66 22 88 66 217.12 2.71 67 22 89 67 217.01 2.69 68 22 90 68 216.96 2.69 69 23 34 11 283.83 0.46 70 23 62 39 283.69 0.39 71 23 63 40 283.59 0.40 72 23 64 41 283.27 0.53 73 23 65 42 283.27 0.53 74 23 90 67 216.97 2.69 75 23 92 69 216.91 2.67 76 23 245 222 64.20 0.14 77 23 246 223 63.97 0.03 78 23 249 226 52.55 0.53 79 24 65 41 283.28 0.53 80 24 67 43 283.19 0.56 81 24 81 57 217.73 2.68 82 24 90 66 217.12 2.71 83 24 91 67 216.96 2.69 84 25 31 6 283.62 0.43 85 25 34 9 283.93 0.45 86 25 64 39 283.67 0.42 87 25 65 40 283.60 0.43 88 25 66 41 283.25 0.54 89 25 92 67 216.95 2.69 90 26 66 40 283.38 0.52 91 26 67 41 283.23 0.55 92 26 68 42 283.18 0.57 93 26 91 65 217.31 2.74 94 27 30 3 858.83 2.35 95 27 31 4 845.05 3.69 96 27 66 39 283.62 0.42 97 28 31 3 857.99 2.78 98 28 49 21 283.57 0.43 99 28 51 23 283.61 0.42 100 28 66 38 283.69 0.42 101 30 33 3 868.46 2.23 102 30 34 4 852.63 5.12 103 30 238 208 66.67 0.14 104 31 34 3 866.04 4.03 105 31 35 4 853.30 4.79 106 31 51 20 284.12 0.50 107 31 53 22 284.19 0.53 108 31 64 33 284.13 0.49 109 31 66 35 283.88 0.42 110 32 35 3 865.46 3.73 111 32 36 4 853.30 4.79 112 32 53 21 284.22 0.51 113 33 36 3 858.33 7.30 114 33 37 4 853.31 4.79 115 33 235 202 66.85 0.20 116 33 242 209 66.61 0.13 117 34 37 3 859.60 1.65 118 34 38 4 846.07 1.17 119 34 64 30 284.30 0.46 120 34 92 58 216.93 2.69 121 34 242 208 66.61 0.13 122 35 38 3 858.74 0.36 123 35 41 6 284.57 0.48 124 35 53 18 284.33 0.48 125 35 55 20 284.56 0.50 126 35 64 29 284.30 0.46 127 36 41 5 400.98 50.38 128 37 242 205 66.64 0.13 129 37 246 209 64.20 0.14 130 38 55 17 355.11 0.75 131 38 56 18 355.94 0.37 132 38 57 19 355.99 0.35 133 38 62 24 355.06 0.77 134 38 64 26 339.93 1.38 135 38 246 208 64.19 0.14 136 39 55 16 356.06 0.36 137 39 56 17 356.06 0.36 138 39 57 18 356.13 0.35 139 40 54 14 356.48 0.31 140 40 55 15 356.36 0.29 141 40 56 16 356.27 0.34 142 41 53 12 356.56 0.29 143 41 54 13 356.48 0.31 144 41 55 14 356.37 0.29 145 42 53 11 356.56 0.28 146 42 54 12 356.49 0.30 147 43 53 10 356.56 0.29 148 44 52 8 356.63 0.27 149 44 53 9 356.60 0.29 150 44 54 10 356.58 0.29 151 45 51 6 356.77 0.28 152 45 52 7 356.72 0.29 153 46 50 4 816.02 10.37 154 46 51 5 356.78 0.28 155 47 50 3 863.42 1.99 156 49 66 17 356.38 0.30 157 50 65 15 356.38 0.30 158 50 66 16 356.35 0.32 159 50 67 17 356.33 0.32 160 50 76 26 221.28 1.10 161 50 77 27 217.95 2.57 162 51 65 14 356.37 0.30 163 51 66 15 356.33 0.32 164 52 63 11 356.43 0.32 165 52 64 12 356.38 0.30 166 52 65 13 356.25 0.31 167 53 63 10 356.38 0.30 168 53 64 11 356.32 0.32 169 54 63 9 356.32 0.32 170 55 61 6 356.68 0.38 171 55 62 7 356.52 0.36 172 56 59 3 881.57 4.34 173 56 60 4 809.70 9.23 174 56 61 5 356.68 0.38 175 57 60 3 845.43 2.76 176 57 62 5 705.36 42.73 177 57 246 189 64.22 0.14 178 57 250 193 52.62 0.51 179 57 253 196 38.81 0.07 180 58 250 192 52.84 0.46 181 60 253 193 35.90 1.53 182 62 253 191 34.88 1.02 183 65 81 16 217.72 2.67 184 67 73 6 448.76 76.78 185 67 74 7 218.11 2.73 186 67 77 10 217.88 2.62 187 67 78 11 217.88 2.61 188 67 91 24 217.34 2.75 189 68 73 5 725.35 36.17 190 69 73 4 834.69 2.66 191 69 197 128 217.25 2.77 192 69 198 129 217.33 2.77 193 70 73 3 869.40 4.03 194 70 74 4 845.63 2.06 195 70 197 127 217.31 2.77 196 70 198 128 217.36 2.77 197 71 74 3 876.05 0.70 198 71 75 4 837.36 2.08 199 71 201 130 217.40 2.81 200 72 75 3 844.94 1.33 201 72 76 4 847.54 3.02 202 73 76 3 847.92 2.83 203 73 77 4 846.80 3.39 204 74 77 3 845.85 2.03 205 74 78 4 844.96 2.46 206 74 198 124 217.47 2.71 207 74 205 131 217.78 2.71 208 75 78 3 861.77 1.66 209 75 79 4 840.54 5.01 210 75 201 126 217.66 2.69 211 75 205 130 217.76 2.71 212 76 79 3 861.76 1.66 213 76 80 4 849.66 0.45 214 77 80 3 862.00 2.89 215 77 81 4 831.53 0.48 216 78 81 3 844.37 2.06 217 78 82 4 832.20 0.14 218 78 89 11 217.93 2.73 219 78 205 127 217.96 2.69 220 78 206 128 217.98 2.69 221 79 82 3 860.70 5.54 222 79 83 4 845.72 1.39 223 80 83 3 848.16 2.61 224 80 84 4 845.71 1.39 225 81 84 3 839.49 4.50 226 81 85 4 829.70 0.39 227 81 86 5 799.32 0.19 228 81 89 8 722.50 38.13 229 82 85 3 852.24 4.66 230 82 86 4 799.25 0.23 231 82 89 7 787.02 5.88 232 82 211 129 384.39 4.71 233 86 89 3 974.55 0.46 234 87 211 124 485.63 1.71 235 87 212 125 485.63 1.71 236 88 136 48 485.64 1.71 237 88 212 124 485.63 1.71 238 88 252 164 39.77 0.41 239 89 202 113 485.76 1.68 240 89 206 117 486.10 1.62 241 89 211 122 485.99 1.60 242 89 212 123 485.78 1.68 243 89 213 124 485.64 1.71 244 89 214 125 485.64 1.71 245 90 136 46 485.75 1.69 246 90 140 50 485.63 1.71 247 90 214 124 485.64 1.71 248 90 245 155 64.25 0.16 249 90 248 158 53.69 0.04 250 90 249 159 52.55 0.53 251 90 252 162 41.40 1.22 252 91 196 105 485.87 1.62 253 91 198 107 486.04 1.70 254 91 202 111 486.18 1.64 255 91 214 123 485.79 1.68 256 91 215 124 485.65 1.71 257 91 216 125 485.64 1.71 258 92 216 124 485.64 1.71 259 92 241 149 66.58 0.18 260 92 242 150 66.55 0.17 261 92 245 153 64.20 0.14 262 93 216 123 485.84 1.66 263 93 217 124 485.65 1.71 264 93 218 125 485.64 1.71 265 94 217 123 485.75 1.69 266 94 218 124 485.67 1.70 267 94 220 126 485.30 1.78 268 94 238 144 66.69 0.14 269 95 108 13 486.06 1.62 270 95 194 99 486.04 1.61 271 95 195 100 486.13 1.66 272 95 196 101 486.17 1.65 273 95 217 122 485.93 1.61 274 95 218 123 485.74 1.68 275 95 219 124 485.64 1.71 276 95 220 125 485.59 1.72 277 95 221 126 485.29 1.78 278 96 194 98 486.16 1.65 279 96 195 99 486.17 1.65 280 96 221 125 485.12 1.80 281 97 102 5 485.88 1.64 282 97 193 96 486.16 1.65 283 97 194 97 486.14 1.65 284 97 219 122 485.97 1.60 285 97 221 124 485.60 1.72 286 97 222 125 466.69 0.24 287 97 223 126 463.91 1.33 288 98 184 86 486.04 1.61 289 98 188 90 486.04 1.61 290 98 192 94 486.42 1.75 291 98 193 95 486.33 1.71 292 98 194 96 486.51 1.77 293 98 195 97 486.61 1.74 294 98 223 125 467.22 0.01 295 98 224 126 461.64 0.21 296 99 102 3 855.45 2.81 297 99 188 89 486.13 1.62 298 99 192 93 486.59 1.73 299 99 193 94 486.54 1.76 300 99 222 123 485.76 1.62 301 99 224 125 465.47 0.55 302 100 188 88 486.61 1.75 303 100 224 124 480.86 0.28 304 101 152 51 486.67 1.70 305 102 106 4 820.15 6.69 306 102 108 6 707.52 40.79 307 102 152 50 486.61 1.72 308 102 193 91 486.80 1.72 309 102 219 117 486.54 1.61 310 102 222 120 485.88 1.67 311 103 106 3 861.82 4.69 312 103 219 116 486.56 1.61 313 103 225 122 458.56 0.43 314 104 219 115 486.56 1.61 315 104 220 116 486.54 1.61 316 104 221 117 486.28 1.64 317 104 222 118 486.21 1.65 318 104 225 121 457.39 0.16 319 104 233 129 67.38 0.23 320 104 234 130 67.33 0.21 321 105 219 114 486.69 1.65 322 105 237 132 67.04 0.18 323 106 150 44 761.01 0.57 324 106 152 46 760.39 0.65 325 106 218 112 486.94 1.60 326 106 219 113 486.81 1.61 327 107 149 42 761.19 0.51 328 107 150 43 761.12 0.52 329 107 151 44 760.79 0.46 330 107 152 45 760.81 0.45 331 107 216 109 488.17 1.09 332 107 217 110 487.28 1.51 333 107 218 111 486.94 1.60 334 107 241 134 66.69 0.14 335 107 244 137 66.67 0.14 336 108 152 44 760.83 0.44 337 108 193 85 579.86 1.07 338 108 194 86 555.04 0.12 339 108 216 108 488.18 1.09 340 108 217 109 487.40 1.46 341 108 219 111 487.28 1.51 342 109 137 28 761.52 0.58 343 109 140 31 761.49 0.58 344 109 141 32 761.47 0.56 345 109 151 42 761.24 0.53 346 109 152 43 761.22 0.54 347 109 153 44 761.11 0.53 348 109 154 45 761.04 0.50 349 109 193 84 586.41 0.58 350 109 215 106 488.95 0.80 351 109 216 107 488.21 1.09 352 110 113 3 914.32 3.96 353 110 132 22 761.81 0.56 354 110 154 44 761.17 0.56 355 110 155 45 760.64 0.53 356 110 156 46 757.17 0.77 357 110 193 83 586.61 0.66 358 110 203 93 541.74 1.81 359 110 215 105 489.00 0.81 360 111 131 20 761.87 0.57 361 111 132 21 761.81 0.56 362 111 153 42 761.27 0.54 363 111 154 43 761.24 0.55 364 111 155 44 761.23 0.55 365 112 130 18 761.93 0.58 366 112 131 19 761.87 0.57 367 112 155 43 761.24 0.55 368 112 156 44 761.23 0.55 369 112 203 91 542.73 2.31 370 113 130 17 762.01 0.60 371 113 156 43 761.23 0.55 372 113 157 44 761.23 0.55 373 113 199 86 545.19 2.31 374 114 156 42 761.26 0.55 375 114 157 43 761.22 0.55 376 114 158 44 761.22 0.55 377 114 187 73 586.79 0.67 378 114 192 78 586.78 0.66 379 115 120 5 798.99 8.85 380 115 121 6 791.41 5.07 381 115 124 9 762.47 0.59 382 115 125 10 762.40 0.59 383 115 126 11 762.09 0.56 384 115 128 13 762.19 0.61 385 115 130 15 762.07 0.58 386 115 204 89 545.08 1.98 387 116 157 41 761.28 0.54 388 116 158 42 761.24 0.54 389 116 159 43 761.22 0.55 390 116 160 44 761.22 0.55 391 116 179 63 587.15 0.58 392 116 187 71 586.95 0.67 393 117 120 3 904.86 0.42 394 117 158 41 761.27 0.54 395 117 160 43 761.22 0.55 396 117 200 83 579.77 1.01 397 118 121 3 882.93 5.79 398 118 160 42 761.24 0.54 399 119 122 3 877.12 2.89 400 119 201 82 567.35 2.45 401 120 123 3 848.99 1.53 402 120 124 4 838.62 3.05 403 120 201 81 563.00 4.63 404 120 204 84 545.34 2.02 405 121 124 3 862.12 8.09 406 121 126 5 762.88 0.68 407 123 201 78 576.41 1.71 408 123 205 82 545.27 2.02 409 123 208 85 544.60 1.80 410 123 209 86 544.60 1.80 411 124 128 4 825.03 13.05 412 124 204 80 545.31 2.01 413 124 208 84 544.45 1.77 414 125 128 3 851.61 0.24 415 128 207 79 543.79 2.09 416 128 208 80 543.34 2.25 417 130 203 73 542.88 2.39 418 130 204 74 541.75 1.82 419 131 203 72 542.73 2.31 420 131 207 76 535.54 1.08 421 132 137 5 828.19 8.39 422 132 203 71 541.74 1.82 423 132 213 81 489.81 0.83 424 132 214 82 489.44 0.79 425 133 136 3 844.99 1.18 426 133 137 4 839.94 2.54 427 133 212 79 490.01 0.80 428 133 213 80 489.98 0.79 429 134 137 3 844.57 0.97 430 134 138 4 845.43 0.49 431 135 138 3 856.03 2.21 432 135 139 4 845.43 0.48 433 135 212 77 490.03 0.80 434 135 252 117 50.35 0.03 435 136 139 3 855.25 1.81 436 136 140 4 845.45 0.49 437 136 212 76 490.01 0.80 438 136 213 77 489.94 0.78 439 136 214 78 489.91 0.78 440 136 252 116 49.45 0.32 441 137 140 3 860.49 2.79 442 137 141 4 846.96 0.27 443 138 141 3 855.23 4.41 444 138 142 4 836.22 4.11 445 139 143 4 836.27 4.10 446 139 248 109 62.78 0.48 447 139 252 113 50.84 0.01 448 140 143 3 849.33 1.39 449 140 144 4 822.47 2.81 450 140 214 74 489.86 0.77 451 140 215 75 489.85 0.77 452 140 216 76 489.76 0.79 453 140 248 108 61.44 0.62 454 141 144 3 831.10 1.51 455 141 145 4 822.47 2.81 456 141 153 12 761.29 0.55 457 142 145 3 828.64 0.28 458 142 146 4 821.41 3.33 459 142 251 109 63.92 0.02 460 143 146 3 821.35 3.34 461 143 147 4 821.35 3.34 462 143 244 101 66.58 0.17 463 143 247 104 65.94 0.00 464 143 248 105 64.31 0.15 465 143 251 108 63.23 0.28 466 144 148 4 813.13 0.75 467 144 149 5 763.23 0.37 468 144 151 7 761.42 0.49 469 144 244 100 66.64 0.14 470 145 148 3 829.00 4.58 471 146 251 105 65.30 0.32 472 147 240 93 67.03 0.18 473 147 243 96 66.91 0.17 474 147 244 97 66.84 0.17 475 149 244 95 66.86 0.17 476 152 193 41 586.11 0.70 477 153 193 40 586.51 0.62 478 154 193 39 586.58 0.65 479 155 191 36 586.74 0.65 480 155 192 37 586.61 0.66 481 155 193 38 586.62 0.66 482 155 194 39 586.57 0.64 483 156 192 36 586.71 0.65 484 156 194 38 586.56 0.65 485 156 195 39 586.38 0.57 486 156 196 40 586.24 0.63 487 157 192 35 586.78 0.66 488 157 194 37 586.62 0.66 489 157 195 38 586.50 0.62 490 157 196 39 586.25 0.63 491 158 196 38 586.57 0.66 492 158 198 40 585.81 0.60 493 158 199 41 559.97 4.20 494 158 200 42 547.58 1.50 495 159 165 6 588.88 0.42 496 159 166 7 588.58 0.28 497 159 196 37 586.66 0.67 498 159 197 38 586.19 0.61 499 161 164 3 888.72 4.21 500 161 165 4 792.52 10.05 501 161 166 5 792.53 10.04 502 161 197 36 586.72 0.66 503 162 166 4 796.93 7.98 504 162 168 6 603.82 7.70 505 162 197 35 586.73 0.66 506 163 168 5 603.68 7.77 507 163 170 7 603.67 7.77 508 163 171 8 588.71 0.40 509 164 167 3 796.80 7.91 510 164 176 12 587.51 0.55 511 165 180 15 587.51 0.55 512 166 195 29 586.94 0.69 513 166 197 31 586.90 0.68 514 166 223 57 476.53 1.53 515 167 176 9 587.88 0.43 516 167 180 13 587.61 0.53 517 167 181 14 587.62 0.53 518 167 182 15 587.57 0.54 519 167 183 16 587.36 0.58 520 167 184 17 587.32 0.60 521 167 223 56 481.37 0.07 522 168 171 3 906.63 7.67 523 168 176 8 587.89 0.42 524 168 223 55 484.94 1.70 525 169 174 5 588.08 0.38 526 169 176 7 587.89 0.43 527 169 181 12 587.76 0.49 528 171 174 3 827.72 6.99 529 176 180 4 826.70 4.04 530 176 181 5 588.43 0.48 531 177 180 3 857.62 0.90 532 177 181 4 809.20 4.72 533 178 181 3 847.98 3.93 534 180 183 3 835.92 2.18 535 182 185 3 873.05 1.88 536 182 186 4 857.23 0.53 537 183 186 3 859.66 0.15 538 183 187 4 846.38 1.53 539 184 187 3 850.18 0.38 540 184 188 4 838.11 5.62 541 184 195 11 586.98 0.70 542 184 223 39 480.27 0.07 543 185 188 3 837.41 5.96 544 185 189 4 837.39 5.97 545 186 190 4 836.70 6.33 546 187 190 3 846.27 1.54 547 187 191 4 836.69 6.33 548 187 192 5 603.99 6.69 549 187 195 8 587.14 0.73 550 188 191 3 873.85 1.43 551 188 224 36 471.19 3.41 552 194 215 21 488.85 0.80 553 195 223 28 463.90 1.32 554 196 199 3 896.23 4.29 555 196 215 19 488.76 0.76 556 198 201 3 875.19 1.19 557 198 202 4 847.06 1.55 558 199 202 3 866.33 3.21 559 199 203 4 848.53 0.82 560 199 215 16 487.83 0.77 561 200 203 3 868.33 1.39 562 200 204 4 845.78 2.19 563 201 204 3 859.94 4.46 564 201 205 4 831.78 4.82 565 202 205 3 851.72 5.15 566 202 206 4 845.25 1.93 567 202 213 11 489.68 0.70 568 202 214 12 489.20 0.86 569 203 206 3 855.16 3.43 570 203 207 4 845.22 1.93 571 203 213 10 489.61 0.67 572 203 214 11 489.36 0.78 573 204 207 3 866.33 7.29 574 204 208 4 841.85 0.26 575 205 208 3 871.49 4.71 576 205 209 4 869.63 5.64 577 206 209 3 866.26 3.06 578 206 210 4 841.25 0.04 579 206 211 5 556.34 33.97 580 206 212 6 490.02 0.81 581 206 213 7 489.97 0.80 582 212 252 40 48.38 0.45 583 216 241 25 66.66 0.14 584 216 245 29 64.34 0.11 585 218 241 23 66.67 0.14 586 219 222 3 889.18 0.99 587 219 237 18 67.00 0.19 588 220 234 14 66.94 0.15 589 220 237 17 66.94 0.15 590 220 238 18 66.92 0.14 591 222 225 3 828.08 11.94 592 226 229 3 948.53 1.20 593 229 234 5 641.41 6.68 594 230 233 3 862.40 1.20 595 232 236 4 862.72 0.23 596 233 236 3 867.21 1.47 597 233 237 4 845.82 4.38 598 234 237 3 846.39 4.09 599 234 238 4 849.20 2.69 600 235 238 3 859.17 0.45 601 235 239 4 848.28 3.13 602 236 239 3 842.06 0.03 603 236 240 4 831.94 5.09 604 238 241 3 866.49 2.85 605 238 242 4 836.35 8.52 606 239 242 3 849.10 5.87 607 239 243 4 833.13 10.12 608 240 243 3 848.03 12.27 609 240 244 4 833.01 10.06 610 241 244 3 854.52 0.04 611 241 245 4 835.57 8.78 612 242 245 3 867.02 2.50 613 242 246 4 817.94 3.33 614 243 246 3 863.31 4.36 615 243 247 4 813.33 1.03 616 244 247 3 859.61 3.51 617 244 248 4 809.18 0.59 618 245 248 3 828.41 8.60 619 245 249 4 824.14 6.30 620 246 249 3 867.32 2.34 621 246 250 4 848.08 2.96 622 247 250 3 860.16 3.08 623 247 251 4 869.15 1.43 624 248 251 3 879.84 0.71 625 249 252 3 855.31 1.17 626 249 253 4 848.07 2.90 627 250 253 3 873.08 0.11