BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 203 199 171.47 0.62 2 4 204 200 171.46 0.62 3 4 205 201 169.74 0.19 4 4 224 220 119.23 0.27 5 4 227 223 118.58 0.30 6 5 35 30 821.22 2.62 7 5 36 31 821.15 2.62 8 5 37 32 821.14 2.62 9 5 38 33 821.12 2.62 10 5 205 200 171.47 0.63 11 6 36 30 821.18 2.62 12 6 37 31 821.14 2.62 13 6 38 32 821.14 2.62 14 6 221 215 119.20 0.23 15 6 227 221 119.24 0.25 16 6 228 222 119.21 0.24 17 6 229 223 119.22 0.24 18 6 246 240 35.74 0.66 19 6 247 241 22.12 0.53 20 7 38 31 821.16 2.62 21 7 40 33 821.14 2.62 22 7 90 83 357.92 0.26 23 7 124 117 327.82 1.24 24 7 161 154 328.24 1.44 25 7 205 198 176.26 0.68 26 7 207 200 172.77 0.02 27 7 227 220 119.29 0.26 28 7 228 221 119.23 0.24 29 7 229 222 119.22 0.24 30 8 37 29 821.22 2.62 31 8 38 30 821.18 2.62 32 8 39 31 821.16 2.62 33 8 40 32 821.14 2.62 34 8 229 221 119.23 0.24 35 8 231 223 119.20 0.24 36 8 240 232 47.93 0.21 37 8 243 235 39.85 0.42 38 8 244 236 37.44 0.12 39 9 40 31 821.14 2.62 40 9 229 220 119.31 0.26 41 9 230 221 119.22 0.24 42 9 231 222 119.20 0.24 43 10 40 30 821.19 2.62 44 10 41 31 821.16 2.62 45 10 42 32 821.16 2.62 46 10 231 221 119.21 0.24 47 10 235 225 76.64 1.13 48 10 240 230 52.04 1.79 49 11 42 31 821.16 2.62 50 11 64 53 358.44 0.20 51 11 95 84 356.54 0.26 52 11 230 219 119.72 0.17 53 11 231 220 119.28 0.26 54 11 232 221 119.21 0.24 55 11 235 224 84.34 0.31 56 12 15 3 870.35 0.90 57 12 21 9 822.10 2.60 58 12 24 12 822.02 2.56 59 12 25 13 822.02 2.56 60 12 28 16 822.01 2.56 61 12 41 29 821.22 2.61 62 12 42 30 821.18 2.62 63 12 43 31 821.16 2.62 64 12 44 32 821.16 2.62 65 12 64 52 358.70 0.14 66 12 235 223 102.28 2.59 67 12 236 224 84.34 0.31 68 13 44 31 821.17 2.62 69 13 64 51 358.70 0.14 70 13 95 82 358.68 0.15 71 13 97 84 358.65 0.14 72 13 232 219 119.64 0.21 73 14 44 30 821.18 2.62 74 15 44 29 821.20 2.62 75 15 236 221 119.11 0.26 76 16 20 4 837.12 2.00 77 16 21 5 824.20 1.81 78 16 47 31 821.13 2.62 79 17 20 3 871.74 1.59 80 17 21 4 831.49 0.98 81 17 47 30 821.13 2.62 82 18 22 4 837.30 1.90 83 18 47 29 821.11 2.60 84 18 50 32 767.47 5.42 85 19 22 3 859.38 0.01 86 19 23 4 846.81 6.28 87 20 23 3 859.75 0.20 88 20 24 4 846.44 6.46 89 21 24 3 846.47 6.45 90 21 25 4 847.64 5.85 91 21 44 23 821.22 2.61 92 21 47 26 820.68 2.53 93 21 50 29 738.26 2.48 94 21 236 215 111.17 0.33 95 22 25 3 849.67 4.23 96 22 26 4 838.84 1.11 97 22 50 28 726.89 8.17 98 23 26 3 841.91 2.65 99 23 27 4 837.53 0.46 100 24 27 3 851.00 1.80 101 24 28 4 841.70 2.85 102 24 235 211 112.34 0.92 103 24 236 212 95.89 1.94 104 25 28 3 857.46 0.67 105 25 29 4 841.70 2.84 106 25 50 25 708.05 5.51 107 25 51 26 705.97 5.29 108 25 54 29 364.36 0.46 109 25 55 30 364.29 0.43 110 26 29 3 856.31 0.10 111 26 30 4 848.46 1.83 112 27 30 3 857.46 0.68 113 27 31 4 846.38 5.07 114 27 241 214 52.48 1.58 115 28 31 3 848.47 1.83 116 28 41 13 821.22 2.62 117 28 55 27 364.25 0.45 118 28 59 31 359.27 0.33 119 28 240 212 52.06 1.79 120 29 54 25 364.31 0.48 121 29 55 26 364.24 0.46 122 29 56 27 364.20 0.47 123 29 58 29 364.18 0.48 124 31 58 27 364.10 0.50 125 31 241 210 47.51 0.12 126 33 37 4 832.13 7.45 127 33 39 6 821.35 2.55 128 33 58 25 359.00 0.14 129 33 244 211 39.62 0.53 130 34 37 3 852.48 1.25 131 34 244 210 39.62 0.53 132 34 245 211 37.45 0.07 133 34 248 214 25.60 1.09 134 37 244 207 37.46 0.07 135 38 90 52 358.19 0.39 136 38 205 167 176.33 0.67 137 39 59 20 358.82 0.20 138 39 60 21 358.82 0.20 139 39 240 201 47.91 0.21 140 39 244 205 37.46 0.08 141 40 59 19 358.87 0.19 142 40 60 20 358.81 0.20 143 40 62 22 358.57 0.21 144 40 90 50 358.56 0.22 145 41 55 14 363.75 0.39 146 41 59 18 358.98 0.17 147 41 60 19 358.92 0.18 148 41 61 20 358.72 0.14 149 41 62 21 358.72 0.14 150 42 62 20 358.72 0.15 151 42 64 22 358.63 0.18 152 43 47 4 828.90 0.62 153 43 48 5 756.23 30.57 154 43 51 8 439.47 4.54 155 43 61 18 358.96 0.17 156 43 62 19 358.88 0.19 157 43 64 21 358.76 0.14 158 44 47 3 854.84 0.22 159 44 48 4 843.25 5.58 160 44 64 20 358.76 0.14 161 45 48 3 854.10 0.15 162 45 78 33 358.39 0.04 163 47 50 3 862.45 6.43 164 47 51 4 831.26 0.39 165 48 51 3 863.89 4.12 166 48 52 4 833.02 0.19 167 48 61 13 359.67 0.01 168 48 63 15 358.99 0.15 169 48 78 30 362.09 1.40 170 48 86 38 364.08 0.60 171 48 88 40 364.03 0.61 172 49 52 3 858.11 1.23 173 49 53 4 841.74 2.10 174 49 86 37 364.07 0.60 175 50 53 3 856.27 0.33 176 50 54 4 841.75 2.11 177 51 54 3 858.33 1.35 178 51 55 4 839.73 3.12 179 51 61 10 362.61 1.32 180 52 55 3 864.65 5.51 181 52 56 4 870.87 6.08 182 52 57 5 834.61 0.85 183 52 59 7 561.45 64.89 184 52 60 8 426.48 0.22 185 52 61 9 363.78 0.74 186 52 86 34 364.57 0.58 187 52 87 35 364.45 0.60 188 55 59 4 843.39 6.92 189 55 61 6 625.78 97.29 190 56 59 3 865.89 3.06 191 60 89 29 423.06 1.05 192 60 90 30 422.88 0.96 193 61 88 27 423.15 1.07 194 61 89 28 423.12 1.06 195 61 90 29 423.09 1.06 196 62 90 28 423.06 1.04 197 62 91 29 423.07 1.05 198 62 92 30 423.06 1.04 199 63 78 15 425.20 0.46 200 63 83 20 423.80 0.82 201 63 90 27 423.51 0.88 202 63 91 28 423.13 1.06 203 63 92 29 423.13 1.06 204 64 92 28 423.53 0.87 205 65 78 13 425.35 0.43 206 65 83 18 425.20 0.46 207 65 91 26 423.79 0.80 208 65 92 27 423.73 0.78 209 65 93 28 423.74 0.79 210 65 98 33 423.83 0.74 211 65 104 39 423.90 0.72 212 65 112 47 423.99 0.74 213 66 78 12 425.35 0.42 214 66 79 13 425.24 0.45 215 66 80 14 425.21 0.46 216 66 83 17 425.23 0.46 217 66 91 25 423.88 0.77 218 66 93 27 423.87 0.77 219 66 112 46 424.19 0.75 220 67 74 7 425.55 0.46 221 67 77 10 425.45 0.42 222 67 78 11 425.41 0.42 223 67 80 13 425.24 0.45 224 67 108 41 424.24 0.77 225 67 112 45 424.24 0.77 226 68 108 40 424.27 0.76 227 68 111 43 424.24 0.77 228 68 112 44 424.24 0.77 229 68 115 47 424.45 0.69 230 69 74 5 771.26 32.97 231 69 79 10 425.45 0.42 232 69 115 46 425.22 0.44 233 70 79 9 425.47 0.42 234 70 115 45 425.25 0.45 235 74 77 3 867.28 0.35 236 78 82 4 787.66 31.95 237 78 83 5 787.62 31.93 238 79 82 3 857.52 2.98 239 79 83 4 857.53 2.99 240 80 83 3 857.57 2.98 241 80 84 4 857.52 2.98 242 80 115 35 429.43 0.67 243 80 116 36 429.28 0.65 244 80 119 39 433.85 1.41 245 80 121 41 433.04 1.04 246 81 84 3 857.57 2.98 247 81 85 4 857.53 2.98 248 82 85 3 857.59 2.96 249 82 86 4 857.54 2.98 250 83 86 3 856.39 0.53 251 83 87 4 792.01 29.78 252 83 88 5 775.05 25.76 253 83 90 7 648.34 35.02 254 83 91 8 433.84 1.53 255 83 121 38 530.86 23.71 256 84 87 3 853.81 0.97 257 84 88 4 780.22 28.34 258 84 119 35 614.91 9.19 259 84 121 37 573.57 10.36 260 88 121 33 675.93 2.47 261 89 121 32 675.93 2.47 262 89 122 33 675.90 2.46 263 90 121 31 675.94 2.46 264 90 122 32 675.90 2.46 265 90 123 33 675.90 2.46 266 90 124 34 675.89 2.46 267 90 159 69 548.77 0.18 268 90 161 71 548.66 0.18 269 91 116 25 676.06 2.46 270 91 121 30 676.03 2.46 271 91 122 31 676.02 2.45 272 91 123 32 675.93 2.46 273 91 124 33 675.91 2.46 274 92 95 3 910.44 2.14 275 92 124 32 675.91 2.46 276 92 126 34 675.89 2.46 277 93 112 19 676.22 2.43 278 93 113 20 676.19 2.43 279 93 116 23 676.20 2.42 280 93 123 30 676.05 2.45 281 93 124 31 676.03 2.45 282 93 125 32 675.90 2.46 283 93 126 33 675.90 2.46 284 94 99 5 685.36 1.70 285 94 109 15 676.21 2.42 286 94 126 32 675.89 2.46 287 95 98 3 853.83 0.83 288 95 99 4 781.02 31.39 289 95 126 31 675.98 2.43 290 95 165 70 548.49 0.13 291 95 230 135 119.87 0.17 292 96 99 3 847.75 2.21 293 96 100 4 691.03 4.53 294 96 165 69 548.19 0.10 295 96 167 71 543.33 0.93 296 97 101 4 682.00 0.03 297 97 102 5 682.00 0.03 298 98 102 4 677.19 2.38 299 98 103 5 677.19 2.37 300 98 104 6 676.87 2.41 301 99 103 4 676.89 2.43 302 99 104 5 676.84 2.42 303 99 106 7 676.84 2.41 304 99 109 10 676.84 2.41 305 99 126 27 676.10 2.45 306 99 127 28 676.06 2.44 307 99 146 47 549.91 0.14 308 99 150 51 549.85 0.14 309 100 130 30 675.95 2.41 310 100 167 67 547.05 0.56 311 104 108 4 846.75 1.79 312 104 109 5 846.75 1.84 313 104 112 8 773.76 30.26 314 105 108 3 846.78 1.81 315 105 109 4 846.78 1.82 316 106 109 3 857.07 3.29 317 106 110 4 846.81 1.83 318 106 146 40 549.57 0.27 319 106 149 43 549.45 0.21 320 107 110 3 860.20 1.74 321 107 111 4 846.80 1.85 322 108 111 3 847.20 2.14 323 108 112 4 846.72 1.90 324 109 112 3 852.63 0.58 325 109 113 4 852.15 0.82 326 110 113 3 852.16 0.82 327 110 114 4 852.15 0.82 328 110 149 39 549.34 0.20 329 110 153 43 549.25 0.18 330 111 114 3 854.28 0.56 331 111 115 4 844.18 0.65 332 112 115 3 866.14 0.73 333 112 116 4 846.29 3.04 334 113 116 3 866.14 0.73 335 113 117 4 845.83 3.27 336 113 123 10 676.13 2.46 337 113 150 37 549.26 0.19 338 113 153 40 549.23 0.18 339 113 154 41 549.21 0.18 340 114 117 3 863.55 4.23 341 114 118 4 845.82 3.27 342 114 153 39 549.24 0.18 343 115 118 3 864.80 0.51 344 115 119 4 840.87 2.22 345 116 119 3 853.27 0.29 346 116 120 4 837.47 0.96 347 116 121 5 713.03 0.32 348 116 123 7 677.13 2.08 349 117 120 3 854.21 0.77 350 117 121 4 769.73 28.03 351 117 123 6 677.16 2.08 352 117 154 37 549.11 0.22 353 117 159 42 548.81 0.20 354 117 160 43 548.79 0.20 355 120 159 39 548.83 0.18 356 122 159 37 548.68 0.17 357 123 159 36 548.82 0.20 358 123 160 37 548.68 0.18 359 123 161 38 548.67 0.18 360 124 161 37 548.66 0.18 361 124 163 39 548.63 0.18 362 124 207 83 188.16 0.10 363 124 230 106 119.43 0.17 364 125 150 25 549.15 0.24 365 125 161 36 548.81 0.20 366 125 162 37 548.68 0.20 367 125 163 38 548.64 0.18 368 126 163 37 548.65 0.18 369 126 165 39 548.61 0.18 370 126 230 104 119.82 0.16 371 127 143 16 549.83 0.22 372 127 146 19 549.62 0.25 373 127 147 20 549.60 0.24 374 127 150 23 549.55 0.27 375 127 163 36 548.79 0.18 376 127 164 37 548.65 0.18 377 127 165 38 548.64 0.18 378 127 188 61 216.19 0.39 379 128 143 15 549.91 0.18 380 128 146 18 549.77 0.20 381 128 164 36 548.77 0.18 382 128 165 37 548.65 0.18 383 129 133 4 843.13 2.51 384 129 134 5 781.94 28.06 385 129 139 10 550.02 0.18 386 129 142 13 550.00 0.18 387 129 143 14 550.00 0.17 388 129 164 35 548.87 0.20 389 129 167 38 548.54 0.21 390 129 168 39 548.35 0.28 391 130 133 3 865.33 5.60 392 130 134 4 852.51 0.24 393 130 167 37 548.48 0.20 394 130 168 38 547.98 0.23 395 131 134 3 856.47 1.74 396 131 135 4 851.11 0.47 397 131 168 37 547.99 0.23 398 131 172 41 519.54 1.68 399 131 174 43 494.44 0.93 400 132 135 3 856.49 1.75 401 132 136 4 855.06 2.44 402 133 136 3 855.78 2.08 403 133 137 4 849.01 5.46 404 133 138 5 639.18 25.14 405 133 142 9 550.12 0.15 406 134 137 3 856.35 1.79 407 134 139 5 570.87 9.02 408 134 168 34 548.56 0.24 409 134 180 46 222.18 0.66 410 134 184 50 221.47 0.62 411 135 174 39 541.31 2.12 412 138 141 3 860.02 3.08 413 138 142 4 841.15 2.12 414 139 142 3 855.89 0.51 415 139 143 4 841.15 2.12 416 139 184 45 217.47 0.13 417 140 143 3 868.66 1.23 418 140 144 4 788.55 28.43 419 140 187 47 216.47 0.35 420 141 144 3 857.45 3.98 421 141 145 4 842.24 1.42 422 142 145 3 852.16 1.50 423 142 146 4 845.31 2.94 424 143 146 3 853.02 0.92 425 143 147 4 849.97 2.44 426 143 164 21 548.89 0.20 427 143 188 45 216.28 0.42 428 144 147 3 861.44 3.29 429 144 148 4 848.70 3.08 430 144 187 43 216.40 0.40 431 144 191 47 216.12 0.38 432 145 148 3 857.00 5.94 433 145 149 4 848.70 3.08 434 146 149 3 865.78 7.36 435 146 150 4 848.69 3.08 436 147 150 3 852.94 0.94 437 147 151 4 848.65 3.08 438 147 162 15 548.92 0.18 439 147 188 41 216.27 0.42 440 148 151 3 855.85 0.52 441 148 152 4 848.62 3.09 442 148 191 43 216.12 0.38 443 149 152 3 855.82 0.53 444 149 153 4 844.95 4.04 445 150 153 3 857.52 0.27 446 150 154 4 841.84 2.49 447 150 162 12 548.86 0.20 448 151 154 3 849.23 1.90 449 151 157 6 712.60 14.29 450 151 191 40 216.10 0.38 451 154 157 3 897.69 1.42 452 154 160 6 549.11 0.28 453 156 159 3 903.89 0.07 454 157 160 3 844.38 0.37 455 157 192 35 215.08 0.62 456 157 196 39 195.00 1.85 457 157 200 43 189.37 0.42 458 157 204 47 188.60 0.12 459 160 204 44 188.52 0.12 460 161 205 44 188.38 0.07 461 161 207 46 188.18 0.10 462 162 192 30 215.16 0.57 463 162 205 43 188.53 0.12 464 162 206 44 188.19 0.09 465 162 207 45 188.16 0.10 466 163 207 44 188.17 0.10 467 163 230 67 119.50 0.20 468 164 184 20 216.16 0.40 469 164 185 21 216.13 0.39 470 164 188 24 216.12 0.39 471 164 207 43 188.35 0.09 472 164 208 44 188.14 0.09 473 164 209 45 188.07 0.06 474 165 209 44 188.03 0.07 475 166 169 3 863.86 2.89 476 166 170 4 857.66 3.53 477 166 208 42 188.40 0.14 478 166 209 43 188.28 0.09 479 166 210 44 188.01 0.07 480 167 170 3 866.60 0.94 481 168 172 4 719.37 6.81 482 168 174 6 467.75 2.59 483 168 176 8 223.68 0.03 484 168 180 12 222.16 0.65 485 169 172 3 839.89 0.79 486 169 174 5 289.04 2.43 487 169 175 6 243.88 1.90 488 169 176 7 222.27 0.68 489 176 180 4 771.53 26.78 490 176 208 32 189.50 0.20 491 177 180 3 848.95 2.27 492 177 181 4 836.86 3.17 493 178 181 3 848.95 2.27 494 178 182 4 785.76 28.72 495 178 219 41 120.56 0.24 496 178 220 42 120.53 0.23 497 178 223 45 120.54 0.22 498 179 182 3 850.57 1.46 499 179 183 4 790.99 26.10 500 180 183 3 855.40 3.46 501 180 184 4 842.96 0.12 502 181 184 3 860.20 3.37 503 181 185 4 838.09 2.31 504 181 208 27 190.29 0.20 505 181 220 39 120.53 0.23 506 182 185 3 852.78 3.21 507 182 186 4 843.51 1.72 508 182 223 41 120.42 0.18 509 182 224 42 120.42 0.18 510 182 225 43 120.37 0.19 511 183 186 3 863.07 1.93 512 183 187 4 852.63 3.29 513 184 187 3 863.07 1.93 514 184 188 4 846.50 0.22 515 185 188 3 860.53 2.43 516 185 189 4 846.50 0.22 517 185 206 21 190.45 0.26 518 185 207 22 190.44 0.26 519 185 208 23 190.44 0.26 520 185 225 40 120.27 0.24 521 185 226 41 120.25 0.24 522 186 189 3 856.18 4.60 523 186 190 4 846.06 0.72 524 186 225 39 120.23 0.24 525 187 190 3 847.17 0.17 526 187 191 4 845.16 1.18 527 188 191 3 848.00 1.58 528 188 192 4 843.09 2.24 529 188 206 18 190.63 0.16 530 189 192 3 857.88 0.51 531 189 193 4 842.99 2.30 532 189 201 12 191.14 0.10 533 189 204 15 190.81 0.12 534 189 206 17 190.69 0.14 535 189 225 36 120.23 0.24 536 189 227 38 120.19 0.23 537 190 193 3 855.68 5.38 538 190 194 4 842.98 2.31 539 191 194 3 854.59 3.56 540 191 195 4 842.96 2.31 541 191 196 5 842.95 2.30 542 192 195 3 848.31 0.42 543 192 196 4 771.24 33.37 544 192 197 5 764.35 36.80 545 192 200 8 254.89 5.25 546 192 201 9 214.00 11.47 547 192 204 12 191.00 0.01 548 192 206 14 190.74 0.11 549 193 197 4 586.36 1.16 550 193 198 5 272.94 2.71 551 193 201 8 231.22 2.86 552 196 200 4 846.76 3.42 553 197 200 3 860.82 3.58 554 197 201 4 849.22 2.19 555 198 201 3 860.82 3.58 556 198 202 4 849.24 2.20 557 199 202 3 862.19 4.26 558 199 203 4 849.25 2.20 559 200 203 3 857.25 4.74 560 200 204 4 846.79 3.42 561 205 227 22 119.83 0.18 562 206 226 20 120.17 0.24 563 206 227 21 120.12 0.24 564 207 227 20 120.20 0.24 565 207 228 21 119.83 0.18 566 207 229 22 119.77 0.14 567 207 230 23 119.75 0.14 568 208 212 4 887.47 1.82 569 208 220 12 120.52 0.23 570 208 226 18 120.35 0.22 571 208 228 20 120.22 0.24 572 208 229 21 120.11 0.24 573 208 230 22 119.84 0.17 574 209 230 21 119.84 0.16 575 212 216 4 713.21 3.39 576 212 220 8 239.15 2.18 577 212 229 17 120.48 0.18 578 212 231 19 120.19 0.26 579 212 234 22 119.45 0.04 580 213 216 3 787.75 26.37 581 213 217 4 713.21 3.39 582 213 243 30 48.00 0.26 583 214 217 3 861.56 8.68 584 214 239 25 52.09 1.76 585 214 242 28 48.01 0.24 586 214 243 29 47.98 0.25 587 216 219 3 875.49 2.18 588 216 220 4 847.00 1.53 589 217 220 3 857.51 2.09 590 217 221 4 847.00 1.53 591 217 229 12 120.56 0.17 592 217 243 26 45.38 0.44 593 217 246 29 37.41 0.16 594 218 221 3 859.72 2.77 595 218 222 4 847.00 1.53 596 218 246 28 37.41 0.16 597 218 248 30 37.36 0.14 598 219 222 3 853.64 1.38 599 219 223 4 847.00 1.53 600 220 223 3 857.07 2.14 601 220 226 6 228.33 7.65 602 220 229 9 120.52 0.15 603 221 226 5 187.13 33.41 604 221 229 8 120.52 0.15 605 221 246 25 37.18 0.07 606 221 247 26 35.48 0.80 607 223 226 3 787.65 40.81 608 229 243 14 41.75 1.34 609 229 246 17 37.02 0.20 610 231 234 3 881.91 0.38 611 231 235 4 709.46 4.54 612 231 240 9 52.12 1.78 613 231 243 12 47.74 0.30 614 232 235 3 880.88 0.89 615 233 236 3 880.38 3.16 616 234 237 3 862.90 2.18 617 234 239 5 248.93 0.51 618 234 240 6 86.69 17.16 619 234 243 9 161.04 20.01 620 235 240 5 215.92 6.65 621 237 240 3 873.40 3.33 622 238 241 3 856.28 3.69 623 239 242 3 854.31 2.70 624 239 243 4 794.97 32.37 625 240 243 3 849.71 5.00 626 240 244 4 782.05 38.83 627 241 244 3 798.36 30.68 628 241 245 4 789.22 35.24 629 242 245 3 801.11 29.76 630 242 246 4 768.10 32.25 631 243 246 3 768.11 32.25 632 245 248 3 854.38 4.91