BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 101 100 31.66 0.08 2 1 170 169 29.89 0.35 3 1 171 170 29.42 0.10 4 1 172 171 30.89 0.12 5 1 176 175 31.79 0.03 6 1 196 195 32.31 0.06 7 1 197 196 31.89 0.01 8 1 198 197 29.89 0.35 9 2 194 192 32.51 0.01 10 2 195 193 32.40 0.07 11 2 196 194 32.31 0.06 12 2 197 195 32.16 0.12 13 3 8 5 822.35 2.83 14 3 9 6 778.14 8.97 15 3 12 9 754.32 2.94 16 3 196 193 32.36 0.07 17 3 198 195 32.36 0.07 18 3 237 234 32.36 0.07 19 4 8 4 847.92 0.71 20 5 8 3 848.65 0.28 21 5 9 4 847.24 0.37 22 6 9 3 842.09 2.20 23 6 10 4 844.91 0.79 24 7 10 3 853.63 2.44 25 7 11 4 843.09 0.12 26 8 11 3 856.93 2.90 27 8 12 4 844.13 0.64 28 8 101 93 81.55 0.90 29 9 12 3 856.93 2.90 30 9 13 4 843.11 0.12 31 9 237 228 56.94 5.02 32 9 244 235 47.17 0.14 33 10 13 3 849.64 0.82 34 10 14 4 846.52 0.57 35 11 14 3 855.44 1.58 36 11 15 4 846.56 0.56 37 11 101 90 89.28 1.21 38 12 15 3 862.62 7.18 39 12 16 4 846.52 0.58 40 12 101 89 93.22 0.73 41 12 102 90 93.21 0.74 42 12 171 159 89.08 1.22 43 12 198 186 86.01 0.08 44 12 237 225 84.45 0.39 45 12 246 234 62.96 0.39 46 13 16 3 854.14 3.00 47 13 17 4 854.02 3.06 48 13 244 231 68.03 1.71 49 13 246 233 61.59 0.35 50 14 17 3 854.21 2.96 51 14 18 4 845.89 1.19 52 14 98 84 108.39 0.42 53 15 18 3 855.25 2.44 54 15 19 4 847.26 0.33 55 15 52 37 111.62 1.32 56 15 96 81 108.49 0.36 57 15 97 82 108.39 0.42 58 15 98 83 108.39 0.42 59 15 101 86 108.38 0.42 60 15 102 87 108.03 0.24 61 15 103 88 107.95 0.22 62 16 19 3 859.23 2.62 63 16 20 4 839.22 2.16 64 16 103 87 108.05 0.26 65 16 246 230 68.00 1.90 66 16 249 231 64.48 0.50 67 17 20 3 854.51 0.69 68 17 21 4 841.14 1.20 69 17 50 33 128.02 1.72 70 17 246 229 68.52 1.96 71 17 251 232 61.48 0.67 72 18 21 3 857.97 2.44 73 18 22 4 849.25 5.25 74 18 47 29 128.90 2.15 75 18 50 32 128.00 1.71 76 18 52 34 127.75 1.62 77 18 98 80 124.73 0.10 78 19 22 3 861.68 0.96 79 19 23 4 848.56 5.60 80 19 94 75 116.03 0.74 81 19 95 76 116.09 0.71 82 19 96 77 118.53 0.62 83 19 103 84 116.09 0.72 84 19 105 86 113.89 0.24 85 19 249 228 68.22 1.99 86 20 23 3 851.27 4.25 87 20 24 4 838.57 0.59 88 20 105 85 119.62 1.03 89 20 249 227 68.36 1.97 90 20 251 229 66.92 1.72 91 21 24 3 858.25 0.76 92 21 25 4 838.56 0.60 93 21 50 29 138.94 1.50 94 21 251 228 66.31 1.27 95 22 25 3 853.50 2.42 96 22 26 4 842.68 2.65 97 22 43 21 139.88 1.03 98 22 46 24 139.89 1.03 99 22 47 25 139.88 1.03 100 22 94 72 129.06 2.25 101 23 26 3 853.50 2.41 102 23 27 4 847.85 5.24 103 23 94 71 132.43 0.66 104 23 105 82 129.06 2.25 105 23 106 83 129.06 2.25 106 23 107 84 129.06 2.25 107 23 234 211 129.06 2.25 108 23 249 224 68.61 2.00 109 24 27 3 860.89 1.28 110 24 28 4 847.86 5.23 111 24 251 225 68.47 1.99 112 25 28 3 848.66 5.67 113 25 29 4 841.76 2.70 114 25 42 17 142.53 0.85 115 25 46 21 142.03 0.77 116 26 29 3 857.82 0.17 117 26 30 4 841.05 3.06 118 26 39 13 142.55 0.85 119 26 40 14 142.55 0.85 120 26 42 16 142.54 0.85 121 26 43 17 142.53 0.85 122 26 66 40 142.50 0.84 123 26 68 42 142.53 0.86 124 26 92 66 141.34 0.58 125 26 94 68 141.34 0.59 126 27 30 3 844.06 4.24 127 27 92 65 141.77 0.79 128 27 107 80 141.35 0.59 129 27 109 82 141.36 0.59 130 29 34 5 797.80 2.46 131 29 39 10 143.08 0.83 132 29 42 13 143.08 0.83 133 30 34 4 834.27 5.45 134 30 39 9 143.10 0.84 135 30 84 54 183.63 1.28 136 30 90 60 143.02 0.87 137 30 92 62 143.02 0.86 138 30 109 79 143.03 0.87 139 30 111 81 143.78 0.52 140 31 34 3 869.50 3.14 141 31 84 53 184.77 1.04 142 31 111 80 155.03 1.42 143 32 84 52 184.77 1.04 144 32 85 53 184.01 1.25 145 32 86 54 183.88 1.18 146 32 111 79 183.83 1.17 147 33 83 50 209.50 0.58 148 33 84 51 205.06 0.56 149 34 38 4 853.41 3.31 150 34 39 5 845.66 0.54 151 34 83 49 209.93 0.49 152 34 84 50 208.94 0.39 153 35 38 3 861.64 0.80 154 35 39 4 853.42 3.31 155 35 80 45 210.16 0.56 156 35 81 46 210.16 0.56 157 35 82 47 210.10 0.53 158 35 83 48 209.93 0.49 159 36 39 3 848.18 0.69 160 36 40 4 849.33 0.22 161 36 68 32 209.76 0.45 162 36 70 34 210.02 0.52 163 36 79 43 209.91 0.51 164 36 80 44 210.18 0.56 165 36 82 46 210.19 0.56 166 36 84 48 210.18 0.56 167 37 40 3 849.37 0.21 168 37 41 4 855.26 2.60 169 37 79 42 210.13 0.53 170 37 80 43 210.19 0.56 171 38 41 3 855.28 2.60 172 38 42 4 855.30 2.60 173 39 42 3 863.75 1.62 174 39 43 4 856.00 2.94 175 39 68 29 211.45 0.10 176 39 84 45 211.56 0.12 177 40 43 3 865.84 2.67 178 40 44 4 855.97 2.94 179 40 64 24 211.69 0.01 180 40 65 25 211.64 0.01 181 40 66 26 211.46 0.10 182 40 68 28 211.47 0.10 183 40 79 39 211.71 0.02 184 41 44 3 855.98 2.94 185 41 45 4 855.68 3.11 186 42 45 3 866.88 2.48 187 42 46 4 854.22 3.85 188 43 46 3 866.88 2.48 189 43 47 4 853.84 4.04 190 43 65 22 211.90 0.00 191 43 66 23 211.88 0.01 192 44 47 3 864.92 1.50 193 44 48 4 853.85 4.03 194 44 65 21 212.20 0.15 195 45 48 3 862.84 0.46 196 45 49 4 847.33 0.78 197 46 49 3 847.98 0.45 198 46 50 4 847.33 0.78 199 47 50 3 862.87 1.40 200 47 51 4 840.16 4.34 201 47 52 5 727.44 0.42 202 47 54 7 212.68 0.03 203 47 65 18 218.73 1.98 204 47 94 47 212.75 0.03 205 47 96 49 212.57 0.04 206 48 51 3 839.28 4.80 207 50 98 48 228.28 1.81 208 51 98 47 228.28 1.81 209 52 96 44 236.92 4.51 210 52 97 45 233.90 4.63 211 52 98 46 228.28 1.81 212 53 58 5 769.60 8.61 213 53 96 43 238.25 4.74 214 53 97 44 237.93 4.81 215 54 57 3 855.04 0.54 216 54 58 4 818.30 15.25 217 54 65 11 238.71 4.63 218 54 95 41 238.37 4.72 219 54 96 42 238.36 4.72 220 54 97 43 238.41 4.71 221 55 58 3 848.73 1.71 222 55 59 4 817.79 15.50 223 55 95 40 238.38 4.72 224 55 96 41 238.39 4.72 225 55 97 42 238.54 4.65 226 55 102 47 238.39 4.71 227 55 174 119 238.38 4.71 228 56 60 4 740.00 3.51 229 56 61 5 727.27 9.87 230 56 62 6 239.98 4.18 231 56 64 8 239.96 4.19 232 56 65 9 239.91 4.17 233 56 66 10 239.71 4.25 234 56 67 11 239.70 4.26 235 56 95 39 238.74 4.62 236 57 61 4 240.02 4.21 237 57 62 5 240.00 4.19 238 57 64 7 239.97 4.18 239 57 65 8 239.90 4.16 240 58 97 39 238.83 4.62 241 58 102 44 238.84 4.61 242 59 102 43 238.88 4.59 243 59 174 115 238.81 4.60 244 60 219 159 292.77 0.70 245 62 66 4 873.10 4.35 246 62 67 5 812.63 6.95 247 62 76 14 292.93 0.79 248 62 77 15 293.73 0.58 249 62 78 16 293.80 0.62 250 62 79 17 310.38 0.12 251 62 219 157 293.41 0.65 252 63 78 15 294.00 0.70 253 63 219 156 303.34 4.15 254 64 79 15 310.95 2.55 255 66 79 13 319.70 0.08 256 66 93 27 292.67 0.77 257 66 94 28 288.10 0.80 258 67 76 9 319.47 0.07 259 67 77 10 319.77 0.04 260 67 92 25 294.18 0.76 261 67 93 26 292.94 0.89 262 68 77 9 319.79 0.04 263 68 79 11 319.80 0.05 264 68 90 22 313.26 0.71 265 68 91 23 302.89 0.43 266 68 92 24 300.75 0.45 267 69 73 4 319.77 0.04 268 69 74 5 319.76 0.03 269 69 75 6 319.76 0.04 270 69 76 7 319.79 0.04 271 69 77 8 319.79 0.04 272 69 90 21 313.46 0.62 273 69 91 22 310.62 0.58 274 70 73 3 831.73 0.93 275 70 82 12 319.88 0.09 276 70 84 14 319.72 0.08 277 70 88 18 319.20 0.15 278 70 89 19 318.93 0.16 279 70 90 20 315.41 1.57 280 71 82 11 320.64 0.24 281 71 88 17 319.22 0.14 282 71 89 18 319.04 0.21 283 71 119 48 319.19 0.14 284 72 82 10 320.95 0.15 285 72 216 144 320.72 0.26 286 72 222 150 319.89 0.09 287 72 223 151 319.79 0.04 288 72 226 154 319.75 0.06 289 73 77 4 756.23 0.79 290 73 82 9 320.97 0.15 291 73 216 143 320.73 0.26 292 73 220 147 320.58 0.21 293 73 221 148 320.47 0.21 294 73 222 149 319.91 0.07 295 74 165 91 317.75 0.40 296 74 204 130 313.75 0.70 297 74 212 138 320.18 0.11 298 74 221 147 320.54 0.24 299 74 222 148 320.33 0.21 300 74 223 149 320.33 0.20 301 74 229 155 318.85 0.22 302 75 165 90 313.75 0.70 303 75 204 129 313.74 0.71 304 75 220 145 320.99 0.14 305 75 221 146 320.61 0.22 306 76 219 143 321.09 0.13 307 76 220 144 321.00 0.14 308 76 221 145 321.00 0.14 309 77 220 143 321.04 0.15 310 78 217 139 321.18 0.11 311 78 219 141 321.18 0.11 312 84 88 4 817.62 2.48 313 84 90 6 564.96 68.21 314 84 111 27 380.31 2.54 315 85 88 3 867.58 2.69 316 85 111 26 425.02 0.78 317 86 111 25 425.23 0.69 318 86 112 26 425.17 0.66 319 86 113 27 425.15 0.67 320 87 110 23 425.42 0.65 321 87 111 24 425.29 0.67 322 87 113 26 425.16 0.66 323 87 114 27 425.04 0.69 324 87 119 32 425.03 0.72 325 88 110 22 425.44 0.66 326 88 111 23 425.35 0.67 327 88 119 31 425.07 0.70 328 89 108 19 425.67 0.66 329 89 109 20 425.55 0.64 330 89 110 21 425.46 0.65 331 89 119 30 425.07 0.71 332 89 122 33 425.06 0.71 333 89 123 34 424.98 0.71 334 89 228 139 425.08 0.70 335 89 229 140 425.03 0.72 336 90 108 18 425.68 0.66 337 90 109 19 425.64 0.66 338 90 111 21 425.52 0.65 339 91 107 16 425.75 0.69 340 91 108 17 425.68 0.66 341 91 231 140 424.88 0.76 342 92 106 14 425.87 0.70 343 92 107 15 425.85 0.69 344 92 109 17 425.82 0.68 345 93 104 11 426.23 0.70 346 93 105 12 425.97 0.67 347 93 106 13 425.87 0.69 348 93 231 138 425.19 0.69 349 93 233 140 424.55 0.67 350 94 104 10 426.23 0.70 351 94 105 11 426.06 0.70 352 95 103 8 426.42 0.74 353 95 104 9 426.23 0.70 354 95 174 79 425.21 0.69 355 96 102 6 426.68 0.78 356 96 103 7 426.59 0.80 357 97 101 4 562.31 66.94 358 97 102 5 426.68 0.78 359 98 101 3 851.22 4.88 360 100 172 72 425.32 0.67 361 101 171 70 425.28 0.68 362 101 172 71 425.30 0.68 363 102 170 68 425.20 0.68 364 102 171 69 425.18 0.67 365 102 172 70 425.28 0.68 366 102 173 71 425.27 0.69 367 102 174 72 425.27 0.70 368 103 235 132 424.80 0.79 369 103 249 144 67.82 1.97 370 104 168 64 425.18 0.68 371 104 170 66 425.01 0.73 372 104 174 70 425.20 0.69 373 104 200 96 424.83 0.75 374 104 233 129 425.19 0.69 375 104 234 130 424.98 0.70 376 104 235 131 424.50 0.69 377 104 249 143 68.52 1.96 378 105 233 128 424.73 0.68 379 105 234 129 424.54 0.67 380 105 249 142 68.56 1.98 381 106 231 125 425.18 0.68 382 106 232 126 424.96 0.71 383 106 233 127 424.54 0.67 384 107 231 124 425.00 0.73 385 107 232 125 424.64 0.64 386 108 123 15 425.09 0.70 387 108 230 122 425.06 0.70 388 108 231 123 424.88 0.76 389 108 232 124 424.62 0.62 390 109 123 14 425.07 0.70 391 110 114 4 835.63 4.11 392 110 119 9 425.17 0.68 393 110 120 10 425.07 0.70 394 110 123 13 424.99 0.72 395 114 119 5 784.85 17.99 396 116 119 3 883.25 1.12 397 116 228 112 500.17 1.55 398 117 120 3 868.75 3.78 399 117 121 4 841.14 4.57 400 118 121 3 849.63 2.33 401 118 122 4 847.08 1.60 402 118 159 41 509.10 0.49 403 118 207 89 506.75 1.19 404 118 228 110 508.10 0.52 405 119 122 3 865.46 1.94 406 119 123 4 847.08 1.60 407 119 228 109 509.00 0.97 408 120 123 3 853.01 0.65 409 120 124 4 844.11 3.05 410 121 124 3 852.26 0.43 411 121 125 4 838.37 0.18 412 121 157 36 532.05 0.04 413 121 159 38 531.93 0.00 414 122 125 3 851.26 0.06 415 122 126 4 838.36 0.18 416 122 159 37 532.03 0.05 417 122 205 83 522.39 0.03 418 122 206 84 531.40 0.21 419 122 207 85 532.03 0.06 420 122 228 106 532.11 0.10 421 122 229 107 529.29 0.07 422 122 230 108 519.64 1.40 423 123 126 3 851.37 0.03 424 123 127 4 838.01 1.54 425 123 230 107 527.60 0.90 426 124 127 3 856.18 2.38 427 124 128 4 838.03 1.53 428 125 128 3 855.49 2.73 429 125 129 4 838.08 1.51 430 125 153 28 532.21 0.03 431 125 154 29 533.00 0.23 432 125 157 32 533.38 0.34 433 125 159 34 533.51 0.40 434 125 205 80 532.58 0.03 435 126 129 3 856.23 2.37 436 126 130 4 839.89 2.12 437 126 203 77 532.11 0.04 438 126 205 79 532.61 0.04 439 126 230 104 532.59 0.03 440 126 231 105 532.26 0.07 441 126 232 106 532.05 0.04 442 127 130 3 859.88 0.74 443 127 131 4 839.90 2.12 444 127 232 105 532.25 0.09 445 128 131 3 854.48 1.96 446 128 132 4 840.25 0.99 447 128 153 25 533.72 0.49 448 128 157 29 534.41 0.33 449 129 132 3 841.85 1.15 450 129 133 4 840.25 0.99 451 129 150 21 533.36 0.34 452 129 153 24 533.82 0.46 453 129 203 74 533.49 0.35 454 129 205 76 533.86 0.31 455 130 133 3 841.23 1.48 456 130 134 4 849.32 5.51 457 130 201 71 533.29 0.35 458 130 203 73 533.64 0.35 459 130 232 102 533.63 0.37 460 130 234 104 533.45 0.37 461 130 236 106 533.29 0.35 462 130 245 115 76.53 1.14 463 131 134 3 854.45 3.98 464 131 135 4 849.33 5.50 465 132 135 3 872.07 1.04 466 132 136 4 853.93 4.24 467 132 149 17 534.36 0.40 468 132 153 21 534.50 0.34 469 133 136 3 872.14 4.87 470 133 137 4 853.92 4.24 471 133 146 13 534.19 0.45 472 133 149 16 534.38 0.39 473 133 150 17 534.40 0.38 474 133 201 68 534.00 0.36 475 134 137 3 848.33 4.33 476 134 201 67 534.02 0.36 477 134 236 102 533.85 0.30 478 134 243 109 77.24 0.99 479 134 245 111 77.09 0.93 480 136 141 5 705.52 1.95 481 136 145 9 534.83 0.65 482 136 149 13 535.03 0.54 483 137 146 9 534.98 0.58 484 137 193 56 679.87 1.35 485 137 201 64 534.81 0.51 486 137 236 99 533.95 0.33 487 137 238 101 532.19 0.23 488 137 243 106 77.93 1.12 489 138 141 3 850.71 3.29 490 138 193 55 689.70 0.53 491 138 238 100 533.65 0.35 492 138 240 102 512.14 0.33 493 139 193 54 690.69 0.61 494 139 194 55 690.73 0.58 495 139 195 56 690.49 0.47 496 139 238 99 533.85 0.30 497 139 239 100 533.78 0.32 498 139 240 101 532.62 0.01 499 140 192 52 693.29 1.06 500 140 193 53 692.83 1.19 501 141 192 51 693.42 1.08 502 141 193 52 693.35 1.08 503 142 145 3 867.19 5.66 504 142 191 49 693.54 1.14 505 142 192 50 693.44 1.09 506 143 146 3 872.94 2.78 507 143 178 35 692.97 1.16 508 143 180 37 693.19 1.07 509 143 181 38 691.78 0.45 510 143 189 46 694.00 1.13 511 143 191 48 693.62 1.17 512 143 193 50 693.45 1.09 513 143 199 56 692.98 1.17 514 144 147 3 860.40 3.44 515 144 148 4 838.41 2.57 516 144 185 41 694.03 1.15 517 144 186 42 694.03 1.15 518 144 189 45 694.01 1.12 519 145 148 3 854.68 0.28 520 145 149 4 838.46 2.57 521 146 149 3 852.92 0.61 522 146 150 4 842.55 4.57 523 146 181 35 693.91 1.08 524 146 193 47 694.03 1.12 525 146 199 53 693.54 1.14 526 146 201 55 692.99 1.19 527 147 150 3 852.91 0.61 528 147 151 4 845.52 6.06 529 147 182 35 693.94 1.08 530 147 183 36 694.06 1.13 531 147 184 37 694.12 1.10 532 147 185 38 694.18 1.12 533 148 151 3 857.16 2.29 534 148 152 4 842.57 4.55 535 148 185 37 694.19 1.13 536 149 152 3 854.69 1.48 537 149 153 4 842.56 4.56 538 150 153 3 851.94 4.69 539 150 154 4 847.38 6.96 540 150 161 11 696.24 0.18 541 150 183 33 694.19 1.15 542 150 203 53 692.98 1.19 543 150 205 55 692.75 1.27 544 151 154 3 849.91 5.70 545 151 155 4 842.40 9.46 546 151 161 10 694.29 1.17 547 151 183 32 694.23 1.14 548 151 184 33 694.24 1.14 549 152 155 3 855.09 3.11 550 152 156 4 844.45 8.43 551 153 156 3 847.30 3.97 552 153 157 4 833.50 2.93 553 154 157 3 853.98 0.62 554 154 158 4 833.49 2.93 555 154 159 5 737.86 14.40 556 154 160 6 731.91 17.37 557 154 161 7 696.73 0.00 558 154 205 51 692.93 1.18 559 155 158 3 839.17 1.10 560 159 205 46 693.00 1.18 561 159 206 47 692.87 1.23 562 159 207 48 692.72 1.28 563 160 205 45 693.00 1.19 564 160 206 46 692.78 1.26 565 160 208 48 689.94 0.73 566 161 183 22 695.48 1.37 567 161 184 23 695.81 1.22 568 161 204 43 693.02 1.20 569 161 205 44 692.76 1.29 570 161 210 49 548.85 0.06 571 162 165 3 868.70 2.95 572 162 183 21 695.84 1.21 573 162 204 42 693.04 1.19 574 162 206 44 692.82 1.27 575 162 209 47 582.90 0.17 576 162 210 48 548.85 0.06 577 162 211 49 548.82 0.07 578 162 212 50 548.82 0.07 579 162 229 67 537.94 0.17 580 163 184 21 696.02 1.11 581 163 210 47 548.86 0.07 582 164 211 47 548.84 0.07 583 164 212 48 548.83 0.07 584 165 183 18 696.56 1.23 585 165 204 39 693.12 1.18 586 165 212 47 548.81 0.07 587 165 221 56 542.51 0.65 588 166 180 14 696.82 1.24 589 166 181 15 696.64 1.21 590 166 182 16 696.63 1.21 591 166 202 36 693.48 1.19 592 167 180 13 696.84 1.23 593 167 181 14 696.66 1.23 594 167 200 33 694.40 1.10 595 167 202 35 693.47 1.19 596 168 174 6 707.26 1.04 597 168 175 7 701.12 1.73 598 168 178 10 699.42 1.48 599 168 179 11 697.89 1.24 600 168 181 13 696.83 1.23 601 168 199 31 695.73 1.17 602 168 200 32 695.03 1.42 603 168 202 34 694.86 1.33 604 168 233 65 536.71 0.20 605 169 175 6 703.59 0.71 606 169 176 7 700.29 1.35 607 169 177 8 699.81 1.47 608 169 178 9 699.44 1.48 609 169 198 29 695.90 1.12 610 169 199 30 695.84 1.11 611 170 173 3 879.39 0.78 612 170 174 4 795.69 4.63 613 170 176 6 703.62 0.70 614 170 198 28 695.91 1.12 615 170 199 29 695.90 1.12 616 170 200 30 695.88 1.12 617 170 235 65 535.74 0.35 618 171 176 5 814.11 9.54 619 172 176 4 867.48 1.95 620 173 176 3 894.22 2.19 621 175 179 4 851.14 2.12 622 178 181 3 909.00 0.01 623 178 197 19 695.97 1.10 624 178 199 21 695.89 1.11 625 181 199 18 695.61 1.20 626 181 201 20 693.40 1.22 627 182 186 4 863.01 1.38 628 183 203 20 693.00 1.20 629 183 204 21 692.99 1.20 630 186 189 3 848.61 4.71 631 192 197 5 845.68 1.23 632 193 197 4 812.95 7.91 633 193 199 6 740.93 6.37 634 193 238 45 533.90 0.33 635 194 197 3 850.00 3.39 636 194 238 44 534.35 0.34 637 195 238 43 534.46 0.29 638 195 239 44 533.81 0.33 639 195 240 45 533.81 0.33 640 196 237 41 534.89 0.32 641 196 238 42 534.65 0.21 642 197 237 40 534.88 0.32 643 197 238 41 534.66 0.21 644 198 235 37 535.50 0.36 645 198 236 38 535.15 0.29 646 198 237 39 534.96 0.35 647 199 235 36 535.53 0.37 648 199 236 37 535.15 0.29 649 200 233 33 536.39 0.27 650 200 234 34 536.22 0.29 651 200 235 35 535.53 0.37 652 201 232 31 536.71 0.20 653 201 233 32 536.43 0.28 654 201 234 33 536.22 0.29 655 201 236 35 535.64 0.32 656 202 231 29 536.78 0.19 657 202 232 30 536.71 0.20 658 202 233 31 536.39 0.26 659 203 231 28 536.77 0.18 660 203 232 29 536.74 0.17 661 204 229 25 537.92 0.17 662 204 230 26 537.47 0.09 663 204 231 27 536.78 0.18 664 205 229 24 537.93 0.17 665 205 230 25 537.79 0.12 666 206 209 3 873.23 3.69 667 206 227 21 538.40 0.20 668 206 228 22 538.16 0.15 669 206 229 23 537.94 0.17 670 207 227 20 538.39 0.19 671 207 228 21 538.28 0.14 672 208 225 17 542.25 0.68 673 208 227 19 538.46 0.21 674 209 223 14 542.37 0.72 675 209 225 16 542.21 0.66 676 209 227 18 538.44 0.21 677 211 223 12 542.34 0.68 678 212 221 9 542.55 0.65 679 212 222 10 542.48 0.65 680 212 223 11 542.33 0.69 681 213 221 8 542.58 0.64 682 213 222 9 542.58 0.64 683 214 217 3 931.53 0.93 684 215 220 5 623.21 39.19 685 215 222 7 542.61 0.64 686 216 222 6 623.22 39.19 687 217 220 3 875.91 5.11 688 223 226 3 827.50 6.38 689 223 227 4 714.95 0.90 690 223 229 6 707.37 2.85 691 234 245 11 68.62 2.00 692 234 249 13 68.61 2.00 693 234 250 14 68.59 1.99 694 235 245 10 68.62 2.00 695 235 246 11 68.61 2.00 696 235 249 12 68.52 2.00 697 236 243 7 307.97 117.64 698 236 244 8 68.67 2.00 699 236 245 9 68.62 2.00 700 237 243 6 307.97 117.64 701 237 244 7 68.62 2.00 702 237 246 9 68.61 2.00 703 239 242 3 833.96 11.18 704 239 244 5 623.59 38.58 705 245 250 3 838.03 4.08