BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 6 4 699.51 8.21 2 3 6 3 699.87 8.04 3 3 7 4 723.67 3.87 4 4 7 3 731.38 3.75 5 4 8 4 727.66 1.90 6 5 8 3 727.66 1.90 7 5 9 4 727.66 1.90 8 6 9 3 737.37 0.82 9 6 10 4 731.44 3.79 10 7 10 3 737.37 0.83 11 7 11 4 727.49 5.76 12 8 11 3 728.85 5.08 13 8 12 4 734.31 2.35 14 9 12 3 734.60 1.53 15 9 13 4 730.57 0.48 16 10 13 3 730.57 0.48 17 10 14 4 730.57 0.48 18 11 14 3 724.79 2.41 19 11 15 4 713.46 1.28 20 11 80 69 457.62 0.55 21 11 84 73 489.41 1.75 22 12 15 3 729.01 0.29 23 12 16 4 713.52 1.12 24 12 81 69 457.06 0.83 25 12 84 72 489.13 1.88 26 12 86 74 480.75 0.43 27 13 16 3 740.66 3.55 28 13 17 4 718.01 1.13 29 13 86 73 497.02 3.28 30 13 87 74 497.03 3.28 31 14 17 3 724.74 4.42 32 14 18 4 718.09 1.09 33 15 18 3 718.75 1.43 34 15 19 4 719.88 0.24 35 15 77 62 488.50 3.48 36 15 80 65 498.90 3.39 37 15 81 66 498.90 3.39 38 16 19 3 719.89 0.24 39 16 20 4 720.27 0.43 40 16 81 65 499.73 3.00 41 16 86 70 502.19 1.92 42 16 92 76 492.96 1.25 43 17 20 3 727.49 0.12 44 17 21 4 727.48 0.13 45 18 21 3 743.07 6.22 46 18 22 4 728.65 0.46 47 18 77 59 503.25 1.69 48 19 22 3 756.50 0.49 49 19 23 4 728.65 0.46 50 19 74 55 503.28 1.65 51 19 77 58 503.25 1.69 52 19 78 59 503.24 1.69 53 19 81 62 503.29 1.71 54 19 92 73 503.01 1.79 55 19 95 76 503.00 1.78 56 19 96 77 502.97 1.79 57 20 23 3 729.60 0.01 58 20 24 4 725.43 2.07 59 20 86 66 503.40 1.76 60 20 88 68 503.37 1.77 61 20 95 75 503.01 1.78 62 21 24 3 729.99 0.21 63 21 25 4 718.87 1.75 64 22 25 3 726.25 3.83 65 22 26 4 744.66 7.12 66 22 73 51 503.54 1.76 67 22 74 52 503.49 1.74 68 22 77 55 503.50 1.75 69 23 26 3 747.68 5.61 70 23 27 4 723.25 1.91 71 23 74 51 503.54 1.76 72 23 95 72 503.04 1.77 73 23 99 76 502.20 1.76 74 24 27 3 748.27 5.32 75 24 28 4 723.25 1.91 76 24 95 71 503.21 1.84 77 25 28 3 741.38 1.86 78 25 29 4 721.59 2.75 79 26 29 3 727.45 5.10 80 26 30 4 720.43 1.78 81 26 71 45 503.61 1.78 82 26 74 48 503.62 1.79 83 26 99 73 502.22 1.75 84 26 103 77 501.20 1.97 85 27 30 3 732.10 2.78 86 27 31 4 719.66 1.40 87 27 95 68 503.23 1.84 88 27 99 72 502.45 1.86 89 28 31 3 737.25 8.37 90 29 33 4 748.60 2.98 91 29 63 34 503.97 1.82 92 29 64 35 504.00 1.83 93 29 67 38 503.97 1.83 94 29 103 74 502.06 1.78 95 29 106 77 491.95 0.02 96 30 33 3 737.12 2.76 97 30 102 72 502.12 1.78 98 30 106 76 489.14 1.38 99 32 35 3 729.12 1.08 100 32 36 4 711.02 3.08 101 33 36 3 711.98 3.56 102 33 37 4 711.03 3.09 103 33 60 27 504.26 1.74 104 33 63 30 504.22 1.77 105 34 37 3 733.03 4.03 106 34 38 4 709.59 3.82 107 34 102 68 502.51 1.90 108 34 105 71 501.93 1.78 109 34 106 72 494.23 0.38 110 34 109 75 486.04 0.37 111 35 38 3 726.40 0.71 112 35 39 4 709.58 3.81 113 36 39 3 732.54 0.62 114 37 57 20 504.36 1.77 115 37 60 23 504.36 1.77 116 37 106 69 501.91 1.77 117 37 109 72 486.09 0.36 118 37 110 73 486.01 0.36 119 37 113 76 473.60 0.51 120 38 109 71 491.63 0.12 121 39 113 74 485.45 0.24 122 40 43 3 777.36 1.23 123 41 53 12 504.95 1.80 124 41 113 72 486.06 0.39 125 42 45 3 700.23 2.62 126 42 50 8 505.04 1.77 127 42 53 11 504.98 1.80 128 42 116 74 474.56 0.03 129 42 117 75 473.57 0.50 130 42 120 78 463.23 4.33 131 43 120 77 468.10 2.60 132 43 202 159 129.88 1.60 133 43 203 160 129.42 1.47 134 45 49 4 716.70 0.24 135 45 50 5 642.58 12.23 136 45 124 79 444.75 0.80 137 46 49 3 716.72 0.23 138 46 50 4 716.72 0.23 139 46 124 78 444.72 0.82 140 47 51 4 718.57 0.67 141 47 121 74 465.79 3.78 142 47 124 77 443.14 1.56 143 47 125 78 440.82 2.06 144 47 128 81 411.53 3.50 145 48 51 3 728.35 3.81 146 48 52 4 720.30 0.20 147 49 52 3 724.36 2.23 148 49 53 4 721.31 0.70 149 50 53 3 725.04 2.56 150 50 54 4 721.31 0.70 151 50 117 67 473.56 0.49 152 50 120 70 465.75 3.77 153 50 121 71 456.28 1.42 154 50 124 74 442.96 1.62 155 51 54 3 732.19 2.71 156 51 55 4 727.98 4.81 157 51 121 70 456.74 1.19 158 51 125 74 439.41 1.71 159 52 55 3 728.44 4.58 160 52 56 4 717.82 0.28 161 53 56 3 719.47 0.56 162 53 57 4 715.53 0.54 163 53 113 60 485.46 0.62 164 53 117 64 473.44 0.48 165 54 57 3 719.14 0.07 166 54 58 4 716.79 1.10 167 54 117 63 473.04 0.67 168 54 118 64 473.03 0.67 169 54 121 67 447.95 0.80 170 55 58 3 723.35 1.45 171 55 59 4 719.36 0.17 172 56 59 3 726.44 2.85 173 56 60 4 719.35 0.18 174 57 60 3 721.07 0.17 175 57 61 4 715.58 0.53 176 57 110 53 488.48 1.61 177 57 113 56 478.88 0.50 178 57 114 57 474.09 0.76 179 57 117 60 473.03 0.67 180 58 61 3 731.87 12.72 181 58 62 4 711.53 2.56 182 58 114 56 475.42 1.23 183 59 62 3 740.78 1.22 184 59 63 4 717.66 7.41 185 60 63 3 759.22 0.21 186 60 64 4 719.19 0.83 187 60 110 50 485.95 0.35 188 61 64 3 739.83 9.49 189 61 65 4 719.20 0.82 190 61 110 49 484.27 0.14 191 61 114 53 473.56 0.51 192 62 65 3 735.61 0.75 193 62 66 4 727.31 3.87 194 63 66 3 753.40 0.43 195 63 67 4 743.34 7.69 196 64 67 3 752.18 3.28 197 64 68 4 745.68 6.53 198 64 103 39 502.01 1.72 199 64 106 42 491.98 0.03 200 64 110 46 483.64 0.15 201 65 68 3 749.60 4.57 202 66 69 3 776.12 0.64 203 66 70 4 755.30 1.72 204 67 70 3 755.28 1.71 205 67 71 4 745.66 6.52 206 67 103 36 501.85 1.65 207 68 71 3 749.54 4.55 208 68 72 4 739.15 9.75 209 68 103 35 501.84 1.65 210 69 72 3 733.67 3.39 211 69 73 4 723.29 1.80 212 70 73 3 741.90 0.64 213 70 74 4 722.60 1.26 214 71 74 3 733.35 4.92 215 71 75 4 719.11 0.48 216 71 99 28 502.28 1.72 217 71 100 29 502.12 1.74 218 71 103 32 498.84 1.69 219 72 75 3 726.44 0.40 220 72 76 4 720.77 0.35 221 73 76 3 730.81 2.97 222 73 77 4 720.93 0.42 223 74 77 3 741.78 1.82 224 74 78 4 720.96 0.45 225 74 96 22 503.24 1.82 226 74 99 25 502.16 1.74 227 75 78 3 725.23 0.21 228 75 79 4 720.91 0.47 229 75 96 21 503.25 1.82 230 75 100 25 502.06 1.72 231 76 79 3 723.35 0.74 232 76 80 4 723.35 0.74 233 77 80 3 727.68 1.42 234 77 81 4 717.95 1.95 235 78 81 3 727.67 1.43 236 78 82 4 717.93 1.95 237 78 92 14 503.31 1.82 238 78 93 15 503.29 1.83 239 78 96 18 503.28 1.83 240 79 82 3 730.25 3.19 241 79 83 4 727.03 2.60 242 80 83 3 726.78 2.73 243 80 84 4 706.12 0.19 244 81 84 3 734.31 7.54 245 81 85 4 700.50 2.98 246 81 86 5 516.40 7.88 247 81 89 8 503.45 1.81 248 81 92 11 503.42 1.83 249 82 85 3 700.47 2.97 250 82 89 7 503.94 2.06 251 82 93 11 503.39 1.82 252 86 89 3 690.53 4.75 253 86 92 6 685.07 6.62 254 88 91 3 744.04 3.62 255 88 92 4 717.29 3.26 256 89 92 3 726.46 2.66 257 89 93 4 702.61 4.07 258 90 93 3 730.62 0.58 259 90 94 4 715.25 2.83 260 91 94 3 720.37 0.27 261 91 95 4 720.37 0.26 262 92 95 3 738.76 3.48 263 92 96 4 718.37 0.74 264 93 96 3 724.07 2.10 265 93 97 4 717.90 0.98 266 94 97 3 730.09 1.03 267 94 98 4 723.94 2.04 268 95 98 3 734.01 1.78 269 95 99 4 714.66 0.64 270 96 99 3 735.87 3.89 271 96 100 4 704.89 4.92 272 97 100 3 731.56 2.67 273 97 101 4 709.52 2.79 274 98 101 3 736.09 0.40 275 98 102 4 728.63 4.12 276 99 102 3 735.21 0.83 277 99 103 4 715.87 2.31 278 100 103 3 735.23 0.82 279 100 104 4 729.88 0.28 280 101 104 3 739.95 2.31 281 101 105 4 732.49 1.39 282 102 105 3 738.16 1.45 283 102 106 4 724.88 2.22 284 103 106 3 732.59 2.19 285 103 107 4 722.19 3.57 286 104 107 3 715.75 2.35 287 104 108 4 714.67 1.84 288 105 108 3 728.00 1.08 289 105 109 4 702.18 6.33 290 106 109 3 733.41 3.79 291 106 110 4 715.94 4.16 292 107 110 3 742.71 0.79 293 107 111 4 721.33 1.46 294 108 111 3 750.22 2.79 295 108 112 4 724.52 0.14 296 109 112 3 724.52 0.14 297 109 113 4 724.52 0.14 298 110 113 3 741.09 1.26 299 110 114 4 722.11 1.81 300 111 114 3 742.29 0.66 301 111 115 4 721.80 1.52 302 112 115 3 741.72 0.93 303 112 116 4 721.80 1.52 304 113 116 3 733.34 0.09 305 113 117 4 711.56 6.48 306 114 117 3 721.90 1.31 307 114 118 4 711.54 6.50 308 115 118 3 733.42 4.44 309 115 119 4 719.87 2.33 310 116 119 3 737.72 2.27 311 116 120 4 719.87 2.32 312 117 120 3 742.79 0.26 313 117 121 4 719.87 2.32 314 118 121 3 735.11 3.58 315 118 122 4 734.02 4.12 316 119 122 3 734.02 4.12 317 119 123 4 718.09 12.09 318 120 123 3 729.73 0.25 319 120 124 4 704.60 5.34 320 120 202 82 130.13 1.71 321 121 124 3 728.39 0.93 322 121 125 4 701.93 6.67 323 122 125 3 720.91 2.81 324 122 126 4 701.94 6.67 325 123 126 3 728.25 4.90 326 123 127 4 702.73 4.91 327 123 198 75 130.29 1.61 328 123 201 78 135.86 4.50 329 123 202 79 135.80 4.53 330 124 127 3 734.06 2.00 331 124 128 4 718.97 3.56 332 125 128 3 735.77 2.57 333 125 129 4 719.29 3.54 334 126 129 3 735.28 2.32 335 126 130 4 719.29 3.54 336 126 198 72 136.19 4.54 337 127 130 3 737.78 1.06 338 127 131 4 719.29 3.54 339 127 195 68 135.88 4.38 340 127 198 71 136.22 4.55 341 127 199 72 140.55 6.73 342 128 131 3 731.08 2.29 343 128 132 4 719.24 3.50 344 128 212 84 135.03 5.04 345 129 132 3 734.90 4.20 346 129 133 4 723.43 1.38 347 130 133 3 737.04 0.70 348 130 134 4 722.67 1.00 349 130 191 61 138.33 5.36 350 130 194 64 142.02 7.20 351 130 195 65 141.51 6.98 352 130 198 68 142.00 7.20 353 131 134 3 741.33 1.44 354 131 135 4 722.66 0.99 355 131 195 64 142.02 7.21 356 131 208 77 141.47 7.38 357 131 210 79 141.07 7.40 358 131 211 80 141.06 7.40 359 132 135 3 759.35 5.87 360 132 136 4 718.21 3.22 361 132 212 80 140.96 7.43 362 133 136 3 729.70 8.96 363 133 137 4 719.02 3.62 364 133 191 58 142.40 7.33 365 134 137 3 745.02 1.30 366 134 138 4 713.84 6.23 367 134 188 54 142.50 7.29 368 134 191 57 142.40 7.33 369 134 192 58 142.37 7.32 370 134 210 76 141.38 7.44 371 134 213 79 141.25 7.49 372 134 215 81 141.08 7.49 373 134 217 83 140.22 7.82 374 134 220 86 140.37 7.79 375 135 138 3 712.27 8.18 376 135 139 4 711.00 7.64 377 135 210 75 141.40 7.46 378 135 212 77 141.26 7.49 379 135 213 78 141.07 7.48 380 136 140 4 748.58 2.54 381 137 140 3 748.59 2.54 382 137 141 4 723.62 7.56 383 137 184 47 142.90 7.23 384 137 187 50 142.82 7.21 385 137 188 51 142.78 7.21 386 137 191 54 142.74 7.21 387 138 141 3 736.93 2.08 388 138 142 4 718.66 5.08 389 138 188 50 142.77 7.21 390 138 220 82 140.44 7.79 391 139 142 3 730.12 1.19 392 139 143 4 717.62 5.59 393 140 143 3 736.95 2.08 394 140 144 4 720.41 4.15 395 141 144 3 736.36 0.11 396 141 145 4 713.88 7.42 397 141 181 40 143.21 7.16 398 141 184 43 143.18 7.17 399 141 185 44 143.16 7.17 400 141 220 79 140.52 7.78 401 141 224 83 139.75 7.73 402 142 145 3 733.62 0.62 403 142 146 4 702.88 2.65 404 142 215 73 141.27 7.49 405 142 223 81 140.12 7.72 406 143 146 3 729.72 2.58 407 143 147 4 710.33 6.35 408 144 147 3 710.35 6.36 409 144 148 4 710.35 6.34 410 144 174 30 144.35 7.27 411 144 177 33 143.91 7.28 412 144 181 37 143.37 7.11 413 144 224 80 140.15 7.71 414 144 227 83 128.08 6.16 415 145 148 3 734.68 3.56 416 145 149 4 710.36 6.35 417 145 220 75 141.06 7.57 418 145 223 78 140.38 7.76 419 145 224 79 140.03 7.76 420 145 227 82 125.11 7.62 421 146 149 3 715.39 6.07 422 146 150 4 710.36 6.35 423 146 223 77 140.41 7.78 424 147 151 4 703.78 3.21 425 148 151 3 716.64 3.21 426 148 152 4 698.25 0.45 427 148 174 26 144.47 7.21 428 148 227 79 130.56 7.40 429 149 152 3 733.91 7.13 430 149 153 4 717.15 2.98 431 149 223 74 140.81 7.59 432 149 226 77 139.99 7.75 433 149 227 78 136.77 8.68 434 149 230 81 110.07 0.76 435 150 153 3 749.32 2.48 436 150 154 4 699.47 1.05 437 151 154 3 738.72 7.79 438 151 234 83 96.42 0.18 439 152 171 19 144.60 7.21 440 152 174 22 144.59 7.21 441 152 227 75 139.95 7.63 442 152 230 78 124.51 7.91 443 152 231 79 123.62 8.34 444 152 234 82 94.85 0.59 445 153 230 77 125.10 7.66 446 154 234 80 105.65 2.29 447 156 167 11 157.75 1.55 448 156 234 78 123.44 8.41 449 156 237 81 96.31 0.17 450 156 238 82 94.17 0.30 451 156 241 85 77.09 0.59 452 158 163 5 619.39 29.83 453 158 164 6 211.55 2.15 454 158 167 9 237.50 15.12 455 158 241 83 88.55 1.32 456 159 163 4 730.25 2.93 457 159 245 86 61.31 1.11 458 160 163 3 755.31 0.96 459 160 164 4 740.02 1.96 460 160 245 85 61.22 1.08 461 161 164 3 753.86 0.79 462 161 165 4 723.38 5.90 463 161 245 84 60.90 0.93 464 161 246 85 60.66 0.81 465 162 165 3 734.31 0.90 466 162 166 4 733.85 0.67 467 163 166 3 750.77 0.70 468 163 167 4 733.81 0.65 469 164 167 3 745.13 3.52 470 164 168 4 725.55 3.19 471 164 238 74 93.89 0.27 472 164 241 77 75.38 1.52 473 164 242 78 72.71 0.55 474 164 245 81 60.61 0.79 475 165 168 3 741.46 3.41 476 165 169 4 725.55 3.19 477 166 169 3 722.65 4.63 478 166 170 4 720.37 5.78 479 167 170 3 726.35 2.77 480 167 171 4 709.14 1.03 481 167 238 71 91.54 1.44 482 168 171 3 719.38 3.06 483 168 172 4 710.16 1.54 484 168 238 70 88.80 2.79 485 169 172 3 711.34 0.99 486 169 173 4 710.21 1.55 487 170 173 3 731.99 9.33 488 170 174 4 734.70 1.88 489 171 174 3 738.96 0.26 490 171 175 4 743.52 2.54 491 171 234 63 97.83 0.24 492 171 235 64 96.84 0.35 493 171 238 67 82.75 1.50 494 172 175 3 743.62 2.59 495 173 176 3 751.55 1.37 496 174 177 3 745.58 1.61 497 174 178 4 745.29 1.68 498 175 178 3 745.50 1.57 499 175 179 4 747.41 2.16 500 175 231 56 104.91 1.18 501 176 179 3 775.83 1.68 502 177 180 3 746.83 2.45 503 177 181 4 709.60 0.35 504 178 181 3 744.61 5.34 505 178 182 4 720.51 3.70 506 178 224 46 140.41 7.82 507 178 231 53 100.56 0.53 508 179 182 3 730.92 1.50 509 179 183 4 722.62 2.64 510 180 183 3 720.75 3.58 511 180 184 4 720.75 3.58 512 181 184 3 720.75 3.58 513 181 185 4 720.73 3.58 514 181 224 43 140.32 7.83 515 182 185 3 719.45 2.94 516 182 186 4 719.45 2.94 517 182 224 42 139.78 7.68 518 183 186 3 726.76 6.37 519 183 187 4 723.53 3.18 520 184 187 3 723.53 3.18 521 184 188 4 728.46 5.63 522 185 188 3 733.77 3.44 523 185 189 4 728.46 5.64 524 185 221 36 140.44 7.83 525 185 224 39 139.49 7.59 526 186 189 3 739.71 0.01 527 186 190 4 712.49 2.35 528 187 190 3 724.33 7.70 529 187 191 4 708.37 0.29 530 188 191 3 722.18 6.58 531 188 192 4 708.46 0.30 532 188 217 29 140.46 7.80 533 188 220 32 140.46 7.79 534 189 192 3 724.02 5.66 535 189 193 4 734.73 0.30 536 189 217 28 140.42 7.82 537 189 221 32 140.25 7.83 538 190 193 3 727.28 3.42 539 190 194 4 721.09 6.52 540 191 194 3 738.88 2.39 541 191 195 4 727.32 3.40 542 192 195 3 745.51 1.69 543 192 196 4 717.18 1.67 544 192 217 25 140.05 7.73 545 193 196 3 730.94 4.08 546 193 197 4 714.64 2.94 547 194 197 3 721.65 0.56 548 194 198 4 721.63 0.57 549 195 198 3 737.20 1.91 550 195 199 4 707.29 1.14 551 196 199 3 717.32 3.82 552 196 200 4 710.93 0.70 553 196 208 12 141.44 7.45 554 197 200 3 717.26 3.85 555 197 201 4 710.94 0.70 556 198 201 3 718.78 4.47 557 198 202 4 722.16 2.78 558 199 202 3 764.99 3.10 559 199 203 4 710.90 0.70 560 199 206 7 169.91 1.11 561 199 207 8 164.34 3.90 562 199 208 9 153.88 1.25 563 200 204 4 444.17 73.89 564 203 206 3 776.37 2.10 565 210 213 3 706.34 4.73 566 214 219 5 733.71 4.71 567 215 219 4 729.34 2.52 568 215 220 5 707.72 0.01 569 216 219 3 744.98 2.35 570 216 220 4 718.88 0.56 571 217 220 3 722.14 1.07 572 217 221 4 718.88 0.56 573 218 221 3 731.54 1.39 574 218 222 4 721.07 6.64 575 219 222 3 721.20 6.58 576 219 223 4 721.05 6.63 577 220 224 4 719.91 6.28 578 221 224 3 723.77 4.36 579 221 225 4 717.13 7.67 580 222 225 3 730.08 10.96 581 222 226 4 717.76 7.37 582 223 226 3 726.96 8.09 583 223 227 4 717.76 7.37 584 224 227 3 718.21 5.01 585 224 228 4 708.56 2.75 586 225 228 3 711.93 1.06 587 225 229 4 723.56 4.75 588 226 229 3 741.02 3.98 589 226 230 4 722.15 5.80 590 227 230 3 737.47 1.20 591 227 231 4 734.41 0.32 592 228 231 3 744.10 0.92 593 228 232 4 748.95 1.51 594 229 232 3 778.05 0.51 595 229 233 4 748.95 1.51 596 230 233 3 747.93 0.99 597 230 234 4 734.55 0.25 598 231 234 3 734.54 0.24 599 231 235 4 725.49 4.28 600 232 235 3 725.48 4.29 601 232 236 4 717.96 0.54 602 233 236 3 727.75 3.42 603 233 237 4 718.60 0.85 604 234 237 3 728.18 3.64 605 234 238 4 715.30 0.79 606 235 238 3 722.42 3.35 607 235 239 4 714.10 0.19 608 236 239 3 718.66 0.82 609 236 240 4 706.39 4.04 610 237 240 3 714.53 1.25 611 237 241 4 708.44 1.81 612 238 241 3 722.68 5.25 613 238 242 4 708.54 1.83 614 239 242 3 732.65 0.74 615 239 243 4 708.54 1.83 616 240 243 3 726.50 10.82 617 240 244 4 726.51 10.82 618 241 244 3 739.95 4.11 619 241 245 4 726.51 10.82 620 242 245 3 731.66 8.25 621 242 246 4 725.55 11.30 622 243 247 4 684.32 8.80 623 244 247 3 685.63 8.14 624 244 248 4 684.32 8.80 625 245 248 3 730.68 6.07