BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 7 4 802.91 0.66 2 3 8 5 802.91 0.66 3 3 11 8 788.22 2.55 4 3 147 144 27.41 1.62 5 3 170 167 28.93 0.87 6 3 171 168 29.03 0.92 7 3 172 169 24.13 0.01 8 3 178 175 29.78 0.67 9 4 7 3 817.52 0.38 10 4 8 4 825.60 3.63 11 5 8 3 827.64 1.74 12 5 9 4 824.73 3.20 13 6 9 3 827.64 1.74 14 6 10 4 817.56 0.40 15 7 10 3 832.26 1.92 16 7 11 4 814.35 2.00 17 8 11 3 832.27 1.93 18 8 12 4 806.33 2.02 19 8 172 164 43.51 0.01 20 8 178 170 44.10 0.31 21 8 208 200 45.16 0.83 22 9 12 3 830.68 2.72 23 9 13 4 830.40 2.85 24 10 13 3 817.59 3.56 25 10 14 4 817.61 3.54 26 11 14 3 833.28 1.14 27 11 15 4 817.62 3.54 28 11 178 167 45.13 0.83 29 12 15 3 835.71 0.07 30 12 16 4 817.61 3.54 31 12 208 196 46.09 0.48 32 12 209 197 46.15 0.51 33 12 210 198 45.98 0.45 34 13 16 3 834.12 0.71 35 15 19 4 795.08 0.53 36 15 24 9 657.46 1.90 37 15 178 163 47.33 0.06 38 15 179 164 47.28 0.04 39 15 180 165 46.21 0.57 40 17 180 163 50.05 0.04 41 18 25 7 675.10 5.11 42 18 159 141 54.92 0.70 43 18 180 162 53.15 1.56 44 19 22 3 866.18 2.49 45 19 24 5 746.94 1.22 46 19 25 6 698.46 2.49 47 19 180 161 54.93 0.71 48 21 24 3 837.21 2.42 49 21 25 4 823.02 3.15 50 22 25 3 823.02 3.15 51 22 26 4 768.25 11.86 52 23 168 145 105.78 0.75 53 24 167 143 107.41 1.38 54 24 168 144 105.23 0.48 55 25 156 131 134.84 1.14 56 25 159 134 137.13 0.06 57 25 165 140 133.69 1.22 58 25 166 141 121.50 0.51 59 25 167 142 108.70 0.76 60 25 180 155 121.52 0.51 61 26 165 139 134.63 1.20 62 26 166 140 131.93 2.10 63 27 84 57 519.57 4.79 64 27 85 58 519.58 4.80 65 27 163 136 151.38 3.79 66 27 164 137 134.92 1.14 67 27 165 138 134.86 1.13 68 28 31 3 846.58 2.16 69 28 36 8 657.68 2.29 70 28 84 56 519.72 4.73 71 28 153 125 134.93 1.15 72 28 164 136 153.87 2.54 73 28 166 138 134.92 1.15 74 29 84 55 519.77 4.75 75 30 33 3 902.78 1.49 76 31 36 5 717.09 6.73 77 31 37 6 740.70 5.08 78 31 82 51 520.00 4.65 79 31 83 52 520.03 4.67 80 31 84 53 519.97 4.70 81 31 164 133 361.07 1.97 82 32 37 5 740.71 5.08 83 32 82 50 520.03 4.66 84 32 95 63 520.03 4.67 85 32 96 64 520.03 4.67 86 33 36 3 858.69 0.66 87 33 37 4 812.16 3.60 88 34 37 3 883.43 1.86 89 34 134 100 463.00 0.29 90 35 38 3 840.98 1.79 91 35 191 156 269.15 0.07 92 35 195 160 273.75 1.07 93 36 39 3 839.65 0.34 94 36 153 117 377.68 2.31 95 36 164 128 393.61 0.99 96 36 166 130 384.96 1.30 97 37 81 44 627.14 3.45 98 37 82 45 635.55 1.34 99 37 96 59 637.56 0.18 100 37 133 96 463.82 0.65 101 38 53 15 648.72 1.68 102 38 54 16 647.96 1.78 103 38 79 41 642.32 0.70 104 38 133 95 464.06 0.72 105 38 136 98 462.00 0.59 106 39 51 12 649.63 1.83 107 39 52 13 649.22 1.72 108 39 53 14 648.72 1.68 109 39 81 42 639.40 0.48 110 39 82 43 639.38 0.47 111 39 153 114 399.52 2.09 112 39 155 116 401.50 2.05 113 39 164 125 401.24 2.06 114 39 191 152 314.64 0.47 115 40 51 11 649.66 1.81 116 40 52 12 649.57 1.81 117 40 79 39 642.32 0.70 118 40 80 40 640.99 1.03 119 40 81 41 639.40 0.48 120 40 82 42 639.40 0.48 121 40 155 115 401.51 2.04 122 41 49 8 650.07 1.89 123 41 50 9 649.97 1.88 124 41 51 10 649.64 1.82 125 41 78 37 642.64 0.58 126 41 79 38 642.34 0.70 127 41 80 39 641.91 0.90 128 41 83 42 639.38 0.47 129 41 155 114 401.62 2.01 130 42 49 7 650.09 1.89 131 42 50 8 649.98 1.88 132 42 51 9 649.97 1.88 133 42 52 10 649.81 1.80 134 42 60 18 648.50 1.76 135 42 62 20 648.29 1.80 136 42 66 24 648.21 1.83 137 42 77 35 642.73 0.60 138 42 78 36 642.65 0.58 139 42 79 37 642.37 0.70 140 43 46 3 982.17 0.96 141 43 48 5 685.00 0.60 142 43 49 6 650.09 1.89 143 43 76 33 642.79 0.60 144 43 77 34 642.75 0.60 145 43 78 35 642.65 0.59 146 44 47 3 842.07 1.68 147 44 48 4 699.43 5.35 148 44 66 22 648.25 1.82 149 44 69 25 647.35 1.57 150 44 70 26 646.74 1.77 151 44 76 32 642.79 0.60 152 44 77 33 642.75 0.60 153 45 76 31 642.81 0.60 154 48 69 21 647.45 1.49 155 49 157 108 402.19 1.88 156 49 184 135 329.25 2.25 157 50 66 16 648.09 1.86 158 50 184 134 329.18 2.29 159 50 186 136 328.76 2.12 160 51 62 11 648.26 1.79 161 51 154 103 401.42 2.06 162 51 155 104 401.49 2.06 163 51 184 133 329.18 2.29 164 51 185 134 329.04 2.24 165 51 186 135 328.08 2.40 166 51 187 136 325.23 3.78 167 51 188 137 325.10 3.77 168 52 61 9 648.41 1.75 169 52 62 10 648.25 1.79 170 52 79 27 642.38 0.70 171 52 187 135 325.23 3.78 172 53 60 7 648.46 1.74 173 53 61 8 648.43 1.75 174 53 136 83 464.78 0.48 175 53 187 134 327.37 2.71 176 53 189 136 319.91 2.94 177 53 191 138 320.38 2.69 178 53 197 144 311.23 0.18 179 53 200 147 310.49 0.11 180 54 59 5 674.34 6.12 181 54 60 6 648.47 1.74 182 54 79 25 642.50 0.64 183 54 120 66 608.90 10.59 184 54 129 75 465.59 0.73 185 54 130 76 465.88 0.63 186 54 133 79 465.48 0.74 187 54 135 81 464.82 0.49 188 54 136 82 464.78 0.48 189 55 59 4 787.39 4.54 190 55 61 6 658.12 1.99 191 55 135 80 464.99 0.56 192 55 136 81 464.80 0.48 193 55 138 83 464.78 0.48 194 55 197 142 311.23 0.18 195 55 238 183 131.41 0.09 196 55 242 187 101.23 3.96 197 56 59 3 804.80 4.16 198 56 135 79 465.01 0.55 199 56 242 186 101.23 3.95 200 57 128 71 466.21 0.56 201 57 129 72 466.13 0.58 202 57 130 73 465.90 0.62 203 57 131 74 466.09 0.58 204 57 135 78 465.07 0.57 205 57 245 188 80.67 5.35 206 57 246 189 66.14 2.84 207 58 119 61 609.13 10.53 208 58 120 62 608.95 10.57 209 58 128 70 466.18 0.60 210 58 245 187 80.67 5.35 211 59 118 59 609.30 10.45 212 59 119 60 608.93 10.58 213 59 245 186 80.64 5.36 214 60 79 19 642.45 0.67 215 60 117 57 609.56 10.49 216 60 118 58 609.18 10.51 217 60 120 60 608.90 10.59 218 61 115 54 609.64 10.46 219 61 116 55 609.58 10.48 220 61 117 56 609.26 10.46 221 61 200 139 310.49 0.11 222 62 66 4 807.09 0.96 223 62 115 53 609.65 10.46 224 62 116 54 609.50 10.42 225 62 118 56 609.42 10.44 226 62 187 125 327.37 2.71 227 62 200 138 310.49 0.11 228 63 66 3 816.49 5.66 229 63 67 4 807.95 0.55 230 63 114 51 609.65 10.46 231 63 115 52 609.61 10.44 232 63 116 53 609.59 10.44 233 63 186 123 329.01 2.25 234 63 187 124 328.08 2.40 235 63 200 137 310.50 0.11 236 63 201 138 310.48 0.10 237 63 202 139 310.19 0.05 238 64 67 3 826.99 0.42 239 64 68 4 807.94 0.55 240 64 106 42 610.10 10.63 241 64 107 43 610.09 10.63 242 64 111 47 609.83 10.48 243 64 114 50 609.67 10.44 244 64 115 51 609.59 10.43 245 64 116 52 609.51 10.39 246 65 68 3 829.11 1.21 247 65 69 4 823.71 1.22 248 65 114 49 609.71 10.42 249 65 186 121 329.25 2.25 250 65 202 137 310.42 0.09 251 66 69 3 846.08 0.48 252 66 186 120 329.22 2.27 253 67 70 3 835.05 5.04 254 67 104 37 627.00 2.58 255 67 116 49 608.26 9.89 256 67 118 51 603.94 7.88 257 68 71 3 848.54 1.71 258 68 106 38 610.50 10.71 259 70 73 3 832.37 0.48 260 70 76 6 647.39 2.89 261 70 77 7 645.28 0.64 262 70 104 34 637.67 0.28 263 72 104 32 637.77 0.24 264 72 106 34 636.90 0.12 265 73 76 3 829.60 0.01 266 73 104 31 638.43 0.56 267 74 77 3 830.54 2.81 268 74 101 27 638.81 0.59 269 74 102 28 638.79 0.59 270 74 103 29 638.69 0.56 271 74 104 30 638.40 0.57 272 75 100 25 639.04 0.53 273 77 100 23 638.88 0.56 274 77 101 24 638.71 0.55 275 77 104 27 636.65 0.18 276 77 118 41 587.68 0.15 277 78 98 20 639.03 0.54 278 78 99 21 638.90 0.57 279 78 100 22 638.44 0.56 280 79 98 19 638.95 0.54 281 79 99 20 638.78 0.51 282 79 120 41 582.61 1.17 283 80 83 3 830.63 3.64 284 80 94 14 639.28 0.55 285 80 97 17 639.03 0.54 286 80 98 18 638.68 0.58 287 81 96 15 639.05 0.53 288 81 97 16 639.03 0.54 289 81 99 18 638.65 0.51 290 81 133 52 464.48 0.55 291 82 93 11 640.19 0.33 292 82 95 13 639.58 0.48 293 82 96 14 639.27 0.63 294 82 155 73 401.89 1.91 295 83 92 9 640.39 0.29 296 83 93 10 640.20 0.33 297 83 94 11 639.98 0.35 298 83 155 72 401.90 1.90 299 83 162 79 401.91 1.91 300 84 92 8 640.40 0.29 301 84 93 9 640.34 0.27 302 84 162 78 401.92 1.91 303 84 163 79 401.81 1.86 304 84 164 80 401.79 1.85 305 85 91 6 644.50 1.71 306 85 92 7 640.41 0.28 307 85 93 8 640.41 0.29 308 85 160 75 402.20 1.84 309 85 161 76 402.21 1.85 310 85 162 77 402.17 1.85 311 85 163 78 401.93 1.90 312 86 89 3 827.70 0.68 313 86 90 4 704.12 1.84 314 86 91 5 644.53 1.70 315 87 90 3 797.94 5.17 316 92 162 70 402.12 1.81 317 94 97 3 783.86 9.95 318 96 132 36 464.49 0.54 319 96 133 37 464.48 0.54 320 99 120 21 585.60 0.27 321 99 121 22 582.30 1.32 322 99 122 23 569.68 0.79 323 99 123 24 541.60 8.37 324 99 128 29 466.03 0.61 325 99 129 30 465.92 0.57 326 99 132 33 465.87 0.63 327 100 120 20 585.62 0.26 328 100 121 21 584.61 0.18 329 101 118 17 587.62 0.18 330 101 119 18 587.33 0.04 331 101 120 19 585.63 0.26 332 101 121 20 585.62 0.26 333 102 119 17 587.61 0.18 334 102 120 18 586.42 0.35 335 102 121 19 585.73 0.24 336 103 118 15 587.93 0.10 337 103 119 16 587.62 0.18 338 105 108 3 834.25 0.33 339 105 116 11 608.01 9.64 340 105 117 12 588.56 0.04 341 105 119 14 588.27 0.08 342 105 241 136 125.70 1.36 343 106 115 9 609.59 10.21 344 106 116 10 608.01 9.64 345 107 110 3 821.23 6.20 346 107 111 4 740.71 1.17 347 107 115 8 609.61 10.21 348 108 115 7 622.94 16.84 349 108 116 8 609.70 10.22 350 108 117 9 609.62 10.25 351 108 224 116 142.43 0.80 352 109 113 4 789.00 0.52 353 109 115 6 679.29 7.39 354 109 117 8 609.73 10.23 355 109 220 111 213.19 4.25 356 109 224 115 142.78 0.84 357 109 234 125 141.56 0.23 358 109 237 128 141.56 0.23 359 109 238 129 141.27 0.20 360 109 241 132 141.57 0.37 361 110 113 3 861.57 0.12 362 110 224 114 146.33 0.23 363 113 214 101 243.09 1.54 364 113 218 105 240.61 1.07 365 113 234 121 141.38 0.14 366 114 202 88 310.47 0.10 367 114 214 100 241.47 0.73 368 115 200 85 310.62 0.14 369 115 214 99 240.81 1.01 370 115 216 101 240.66 1.07 371 115 234 119 141.28 0.18 372 115 238 123 137.53 1.30 373 117 238 121 131.88 0.01 374 117 241 124 123.80 0.53 375 122 126 4 734.02 1.24 376 122 127 5 690.23 8.97 377 122 128 6 466.39 0.49 378 123 126 3 815.78 4.41 379 123 127 4 734.87 0.88 380 123 132 9 673.52 0.62 381 127 132 5 777.78 0.54 382 128 131 3 817.10 3.54 383 128 132 4 806.06 1.97 384 129 132 3 806.06 1.97 385 129 133 4 801.02 0.54 386 130 133 3 802.39 1.20 387 130 135 5 737.57 2.98 388 136 191 55 313.93 0.21 389 136 197 61 311.22 0.18 390 137 196 59 311.41 0.12 391 137 197 60 311.37 0.12 392 138 197 59 311.37 0.12 393 138 216 78 239.58 1.20 394 138 235 97 138.35 0.78 395 138 238 100 133.22 0.81 396 138 239 101 131.35 0.05 397 139 142 3 859.67 0.61 398 139 196 57 311.63 0.10 399 139 197 58 311.62 0.09 400 139 198 59 311.47 0.12 401 140 239 99 139.31 1.02 402 142 198 56 311.62 0.09 403 142 216 74 240.45 1.11 404 142 217 75 239.62 1.19 405 143 150 7 756.98 6.00 406 144 148 4 795.79 4.45 407 144 150 6 757.00 5.99 408 144 171 27 400.62 2.18 409 144 174 30 365.18 3.43 410 144 198 54 311.81 0.10 411 144 215 71 242.21 0.77 412 144 217 73 240.34 1.10 413 145 148 3 830.47 6.32 414 145 171 26 400.88 2.23 415 146 171 25 400.69 2.13 416 146 172 26 400.53 2.12 417 146 173 27 400.41 2.08 418 147 170 23 401.25 2.10 419 147 171 24 401.06 2.19 420 148 170 22 401.32 2.08 421 148 171 23 401.26 2.10 422 149 168 19 402.22 1.84 423 149 169 20 402.05 1.77 424 149 170 21 401.34 2.08 425 150 168 18 402.23 1.81 426 150 169 19 402.05 1.77 427 150 171 21 401.60 2.00 428 150 190 40 324.89 3.95 429 150 191 41 324.39 4.09 430 150 192 42 323.24 4.60 431 150 198 48 311.25 0.17 432 151 166 15 402.53 1.85 433 151 167 16 402.47 1.84 434 151 168 17 402.30 1.80 435 151 189 38 325.17 3.81 436 151 190 39 324.83 3.81 437 151 191 40 324.73 3.86 438 152 165 13 403.80 1.71 439 152 166 14 402.78 1.78 440 152 167 15 402.47 1.84 441 152 169 17 402.42 1.85 442 152 177 25 331.84 1.76 443 152 181 29 330.63 2.00 444 152 185 33 329.14 2.31 445 152 188 36 325.16 3.79 446 152 189 37 325.09 3.77 447 152 190 38 324.74 3.85 448 152 201 49 310.30 0.09 449 153 164 11 403.83 1.70 450 153 165 12 403.50 1.86 451 153 166 13 402.81 1.79 452 153 188 35 325.09 3.77 453 153 189 36 325.06 3.77 454 153 191 38 324.72 3.85 455 154 164 10 403.86 1.69 456 154 165 11 403.81 1.71 457 154 185 31 328.68 2.54 458 154 188 34 325.07 3.78 459 155 162 7 404.16 1.80 460 155 163 8 404.01 1.75 461 155 164 9 403.88 1.68 462 155 184 29 329.16 2.29 463 156 159 3 826.90 0.25 464 156 160 4 783.39 1.42 465 156 163 7 538.77 65.50 466 156 165 9 403.87 1.68 467 156 182 26 330.19 1.99 468 156 184 28 329.17 2.29 469 156 185 29 329.15 2.31 470 157 161 4 674.67 2.43 471 157 182 25 330.70 2.00 472 157 183 26 330.65 2.01 473 157 184 27 329.19 2.30 474 158 182 24 330.73 1.99 475 158 183 25 330.71 1.99 476 159 165 6 749.42 10.30 477 159 182 23 330.73 1.99 478 160 163 3 828.02 4.91 479 160 165 5 765.00 5.58 480 167 180 13 330.77 2.01 481 168 179 11 330.85 2.01 482 169 177 8 331.85 1.76 483 169 178 9 331.57 1.87 484 169 179 10 331.33 1.77 485 169 190 21 325.26 3.79 486 170 177 7 331.88 1.76 487 170 178 8 331.58 1.88 488 171 174 3 869.38 0.81 489 171 175 4 816.59 0.77 490 171 176 5 736.63 2.92 491 171 177 6 663.16 3.06 492 171 215 44 253.25 0.86 493 172 176 4 786.35 8.64 494 172 177 5 736.62 2.92 495 172 178 6 736.62 2.92 496 172 207 35 309.60 0.01 497 172 208 36 309.35 0.10 498 172 210 38 253.75 0.91 499 173 176 3 807.07 1.64 500 173 207 34 309.94 0.15 501 174 215 41 253.28 0.84 502 175 212 37 253.54 0.91 503 175 213 38 253.47 0.88 504 175 215 40 253.30 0.84 505 176 205 29 310.22 0.07 506 176 206 30 309.96 0.18 507 176 207 31 309.76 0.26 508 176 210 34 253.74 0.91 509 176 211 35 253.72 0.92 510 176 212 36 253.47 0.88 511 177 201 24 310.76 0.19 512 177 210 33 253.72 0.90 513 177 211 34 253.61 0.90 514 177 212 35 253.50 0.88 515 177 213 36 253.49 0.88 516 177 215 38 253.46 0.87 517 178 210 32 253.68 0.88 518 179 211 32 253.58 0.91 519 181 185 4 776.51 5.33 520 181 201 20 310.23 0.10 521 181 204 23 308.68 0.29 522 181 211 30 253.37 0.91 523 182 185 3 817.16 2.42 524 183 186 3 847.06 0.45 525 185 188 3 811.91 0.80 526 185 201 16 309.99 0.15 527 186 201 15 310.20 0.06 528 186 202 16 309.86 0.17 529 187 200 13 310.47 0.10 530 187 201 14 310.37 0.14 531 188 200 12 310.45 0.10 532 188 201 13 310.13 0.09 533 189 197 8 311.23 0.18 534 189 199 10 310.61 0.14 535 189 200 11 310.47 0.10 536 190 197 7 489.31 88.86 537 190 198 8 311.12 0.15 538 190 199 9 310.61 0.15 539 190 200 10 310.60 0.14 540 190 201 11 310.49 0.11 541 190 215 25 240.14 1.05 542 191 195 4 742.07 13.62 543 191 196 5 658.82 4.12 544 191 197 6 489.31 88.86 545 192 195 3 813.98 7.75 546 192 196 4 742.07 13.62 547 192 198 6 489.31 88.86 548 197 216 19 239.56 1.20 549 198 215 17 240.06 0.97 550 198 216 18 239.56 1.20 551 199 214 15 240.61 1.07 552 199 215 16 239.59 1.20 553 199 216 17 239.57 1.20 554 200 213 13 240.84 1.04 555 200 214 14 240.61 1.07 556 200 216 16 240.22 1.09 557 201 211 10 253.39 0.90 558 201 213 12 240.84 1.04 559 202 212 10 246.51 0.60 560 202 213 11 240.88 1.04 561 202 214 12 240.88 1.03 562 203 211 8 253.39 0.91 563 203 212 9 252.97 0.73 564 204 209 5 253.81 0.85 565 204 210 6 253.72 0.90 566 204 211 7 253.39 0.90 567 204 212 8 253.39 0.90 568 205 209 4 473.78 110.84 569 205 210 5 458.97 103.42 570 205 212 7 253.50 0.93 571 206 209 3 800.75 6.12 572 214 234 20 141.28 0.18 573 215 218 3 838.97 2.60 574 215 234 19 141.25 0.18 575 216 233 17 141.28 0.15 576 216 234 18 139.99 0.81 577 216 235 19 139.72 0.82 578 216 238 22 139.44 0.98 579 217 233 16 141.40 0.21 580 218 233 15 141.84 0.09 581 218 234 16 141.46 0.24 582 219 232 13 141.96 0.12 583 219 233 14 141.86 0.10 584 220 224 4 820.91 0.66 585 220 225 5 780.72 13.49 586 220 232 12 142.09 0.12 587 220 234 14 141.97 0.13 588 220 237 17 142.01 0.12 589 221 224 3 831.97 4.30 590 221 225 4 798.10 4.84 591 222 225 3 799.13 7.87 592 222 228 6 145.50 0.33 593 222 229 7 144.43 0.28 594 222 230 8 143.00 0.09 595 224 241 17 142.32 0.07 596 225 228 3 782.36 0.20 597 225 229 4 717.32 6.28 598 225 237 12 142.28 0.04 599 226 237 11 142.40 0.08 600 226 240 14 142.41 0.06 601 226 241 15 142.34 0.07 602 226 244 18 142.11 0.03 603 227 240 13 142.44 0.07 604 229 232 3 849.31 2.35 605 229 240 11 400.80 127.72 606 232 236 4 817.23 1.23 607 232 240 8 781.77 7.42 608 233 236 3 823.08 1.68 609 233 237 4 807.25 3.76 610 234 237 3 828.61 4.46 611 234 238 4 807.27 3.74 612 235 238 3 817.25 1.24 613 235 239 4 814.13 0.32 614 236 239 3 816.49 0.69 615 236 240 4 815.66 1.08 616 237 240 3 808.82 4.52 617 237 241 4 808.82 4.52 618 238 241 3 816.68 8.45 619 238 242 4 808.82 4.52 620 239 242 3 815.49 1.18 621 239 243 4 806.10 3.35 622 240 243 3 815.95 1.59 623 240 244 4 805.90 3.25 624 241 244 3 817.11 1.23 625 241 245 4 805.90 3.25 626 242 245 3 815.98 1.79