BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 119.00 0.95 2 3 228 225 118.77 0.86 3 3 229 226 116.70 0.62 4 4 204 200 176.34 2.65 5 4 205 201 174.06 2.00 6 4 206 202 173.43 1.68 7 4 207 203 172.01 1.16 8 4 229 225 119.36 0.96 9 5 36 31 388.62 39.34 10 5 37 32 388.52 39.38 11 5 38 33 388.50 39.38 12 5 207 202 173.41 1.69 13 6 36 30 388.85 39.24 14 6 37 31 388.61 39.34 15 6 38 32 388.51 39.38 16 6 223 217 119.72 0.88 17 6 229 223 119.62 0.91 18 6 249 243 20.62 0.35 19 7 37 30 388.85 39.24 20 7 38 31 388.60 39.33 21 7 40 33 388.77 39.24 22 7 90 83 206.24 0.56 23 7 163 156 183.06 1.50 24 7 207 200 179.75 1.50 25 7 209 202 173.37 1.71 26 7 229 222 120.15 0.81 27 7 230 223 119.69 0.92 28 7 231 224 119.64 0.90 29 8 37 29 388.99 39.19 30 8 38 30 388.90 39.22 31 8 39 31 388.69 39.29 32 8 40 32 388.82 39.23 33 8 231 223 119.70 0.92 34 8 242 234 53.26 0.41 35 8 246 238 38.11 0.96 36 9 40 31 388.82 39.23 37 9 124 115 200.52 1.79 38 9 231 222 120.46 0.96 39 9 232 223 119.66 0.91 40 9 233 224 119.62 0.90 41 9 242 233 53.36 0.44 42 10 40 30 388.93 39.22 43 10 41 31 388.84 39.23 44 10 42 32 388.88 39.23 45 10 233 223 119.70 0.92 46 10 237 227 88.11 1.39 47 10 242 232 55.41 0.58 48 11 42 31 388.89 39.23 49 11 65 54 387.19 39.84 50 11 92 81 203.69 1.83 51 11 95 84 202.66 2.06 52 11 232 221 121.86 0.71 53 11 233 222 120.11 0.79 54 11 234 223 119.64 0.91 55 11 237 226 96.17 2.19 56 12 15 3 893.82 0.70 57 12 21 9 391.53 38.88 58 12 24 12 390.58 39.34 59 12 25 13 390.54 39.32 60 12 28 16 390.54 39.32 61 12 41 29 389.10 39.23 62 12 42 30 389.00 39.19 63 12 43 31 388.99 39.20 64 12 51 39 389.36 39.27 65 12 65 53 388.16 39.37 66 12 237 225 118.48 1.35 67 12 238 226 90.83 0.04 68 13 44 31 389.30 39.22 69 13 65 52 388.03 39.43 70 13 95 82 203.00 2.18 71 13 97 84 202.96 2.20 72 13 172 159 203.29 1.72 73 13 234 221 121.84 0.72 74 14 44 30 389.33 39.24 75 15 44 29 389.38 39.26 76 15 238 223 119.50 1.01 77 16 20 4 842.23 0.69 78 16 21 5 837.16 0.04 79 16 47 31 389.55 39.24 80 17 20 3 866.64 1.78 81 17 21 4 840.17 1.51 82 18 21 3 866.52 1.52 83 18 22 4 859.60 1.35 84 18 50 32 390.55 39.33 85 19 22 3 868.75 4.21 86 19 23 4 861.44 1.04 87 20 23 3 876.42 0.38 88 20 24 4 860.84 0.72 89 21 24 3 861.78 0.07 90 21 25 4 860.66 0.63 91 21 47 26 390.55 39.16 92 21 50 29 390.85 39.31 93 21 51 30 390.70 39.24 94 22 25 3 868.94 2.96 95 22 26 4 868.48 3.20 96 22 50 28 390.86 39.31 97 22 54 32 391.06 39.34 98 23 26 3 869.21 2.83 99 23 27 4 868.51 3.18 100 24 27 3 869.20 2.83 101 24 28 4 868.50 3.18 102 24 237 213 117.57 1.49 103 24 238 214 107.03 3.85 104 24 239 215 93.16 0.10 105 24 240 216 87.79 1.56 106 25 28 3 868.49 3.18 107 25 29 4 860.81 0.66 108 25 51 26 390.98 39.30 109 25 54 29 391.33 39.46 110 25 55 30 391.11 39.35 111 26 29 3 865.21 1.54 112 26 30 4 860.81 0.66 113 27 30 3 866.39 2.29 114 27 31 4 861.97 0.07 115 27 240 213 84.59 1.30 116 27 243 216 63.03 3.25 117 28 31 3 858.90 1.46 118 28 57 29 391.78 39.59 119 28 60 32 391.75 39.58 120 28 237 209 107.12 3.79 121 28 242 214 61.43 2.46 122 29 54 25 391.76 39.59 123 29 55 26 391.76 39.59 124 29 56 27 391.77 39.59 125 29 57 28 391.78 39.59 126 31 57 26 391.93 39.54 127 31 243 212 53.23 0.40 128 31 246 215 53.16 0.38 129 31 247 216 52.87 0.40 130 33 37 4 872.62 2.52 131 33 39 6 636.03 96.18 132 33 57 24 550.12 6.96 133 33 59 26 475.70 16.01 134 33 60 27 475.70 16.01 135 33 246 213 52.37 0.28 136 35 59 24 577.05 0.29 137 35 246 211 52.17 0.37 138 35 247 212 49.51 0.63 139 35 249 214 37.08 0.46 140 35 250 215 24.05 0.73 141 36 59 23 577.12 0.31 142 37 59 22 577.15 0.32 143 37 246 209 42.38 0.36 144 37 249 212 32.04 2.85 145 38 59 21 577.15 0.32 146 38 60 22 577.13 0.32 147 38 61 23 577.12 0.31 148 38 90 52 206.26 0.57 149 39 59 20 577.18 0.30 150 39 60 21 577.14 0.32 151 39 61 22 577.12 0.32 152 39 246 207 38.10 0.96 153 40 61 21 577.15 0.33 154 40 63 23 577.10 0.31 155 40 90 50 206.30 0.56 156 40 124 84 197.00 1.75 157 41 51 10 577.34 0.32 158 41 55 14 577.35 0.34 159 41 60 19 577.24 0.30 160 41 61 20 577.20 0.32 161 41 62 21 577.13 0.33 162 41 63 22 577.11 0.32 163 42 63 21 577.11 0.32 164 42 65 23 576.64 0.12 165 42 92 50 206.37 0.59 166 43 47 4 862.22 0.76 167 43 48 5 834.52 0.80 168 43 51 8 577.53 0.35 169 43 62 19 577.23 0.32 170 43 63 20 577.21 0.32 171 43 65 22 577.12 0.32 172 44 47 3 879.94 1.88 173 44 48 4 869.00 2.64 174 44 65 21 577.16 0.32 175 45 48 3 889.29 2.44 176 45 49 4 893.50 0.33 177 45 64 19 577.21 0.32 178 45 66 21 577.19 0.31 179 45 78 33 215.52 0.10 180 46 49 3 925.93 1.51 181 47 50 3 884.92 1.02 182 47 51 4 868.59 2.66 183 48 51 3 874.89 0.48 184 48 52 4 859.45 0.92 185 48 62 14 577.39 0.33 186 48 64 16 577.23 0.32 187 48 83 35 208.81 0.87 188 48 86 38 208.91 0.92 189 48 88 40 207.94 0.61 190 49 52 3 861.39 0.06 191 49 53 4 858.96 1.16 192 50 53 3 867.51 3.12 193 50 54 4 861.38 0.07 194 51 54 3 862.18 5.16 195 51 55 4 835.01 0.48 196 51 62 11 577.40 0.32 197 52 62 10 577.44 0.33 198 52 86 34 207.86 0.46 199 52 88 36 207.44 0.66 200 53 56 3 1065.38 0.43 201 55 60 5 577.65 0.35 202 55 62 7 577.56 0.37 203 57 60 3 868.11 2.24 204 60 242 182 61.41 2.42 205 60 246 186 46.73 0.47 206 61 90 29 206.28 0.56 207 62 88 26 207.12 0.80 208 62 90 28 206.78 0.80 209 63 88 25 207.21 0.81 210 63 90 27 206.93 0.71 211 63 91 28 206.87 0.68 212 63 92 29 206.90 0.70 213 64 83 19 208.12 0.54 214 64 88 24 207.58 0.80 215 64 90 26 207.14 0.80 216 64 91 27 207.12 0.80 217 64 92 28 207.13 0.79 218 65 92 27 207.15 0.80 219 66 91 25 207.48 0.69 220 66 92 26 207.21 0.78 221 66 93 27 207.19 0.79 222 66 98 32 207.28 0.73 223 66 112 46 208.22 0.59 224 67 78 11 215.74 0.19 225 67 80 13 215.30 0.09 226 67 83 16 215.14 0.07 227 67 91 24 207.66 0.64 228 67 93 26 207.65 0.64 229 67 112 45 208.25 0.61 230 68 74 6 223.52 1.81 231 68 77 9 216.59 0.12 232 68 78 10 216.57 0.12 233 68 79 11 215.67 0.16 234 68 80 12 215.75 0.20 235 68 112 44 208.25 0.60 236 69 80 11 216.25 0.06 237 69 81 12 216.57 0.12 238 69 115 46 213.08 0.75 239 69 119 50 216.13 0.12 240 70 74 4 912.64 0.98 241 70 79 9 216.59 0.11 242 70 81 11 216.58 0.12 243 70 119 49 216.51 0.15 244 71 74 3 959.04 2.02 245 71 79 8 216.61 0.10 246 71 81 10 216.61 0.10 247 71 82 11 216.61 0.10 248 74 77 3 882.78 3.06 249 78 82 4 869.90 0.64 250 78 83 5 845.58 0.67 251 79 82 3 869.90 0.64 252 79 83 4 869.90 0.64 253 80 83 3 869.93 0.63 254 80 84 4 857.71 6.74 255 80 93 13 545.38 19.12 256 80 112 32 548.49 17.58 257 80 115 35 557.27 17.40 258 80 116 36 548.51 17.57 259 80 119 39 588.13 2.62 260 80 121 41 588.06 2.62 261 81 84 3 869.91 0.64 262 81 85 4 850.61 3.19 263 81 119 38 591.90 0.74 264 82 85 3 865.62 2.55 265 82 86 4 856.31 6.09 266 83 86 3 867.45 1.63 267 83 87 4 849.88 3.54 268 83 88 5 643.20 24.78 269 83 121 38 600.38 3.28 270 84 87 3 867.45 1.63 271 84 88 4 837.63 1.92 272 84 89 5 636.60 21.39 273 84 119 35 603.87 1.82 274 84 120 36 605.82 0.61 275 84 121 37 603.50 1.73 276 88 121 33 664.97 0.08 277 89 121 32 664.96 0.08 278 89 122 33 664.86 0.08 279 90 121 31 664.97 0.09 280 90 122 32 664.91 0.08 281 90 124 34 664.90 0.11 282 90 163 73 664.94 0.10 283 91 116 25 665.13 0.10 284 91 121 30 665.07 0.10 285 91 122 31 665.02 0.10 286 91 123 32 664.97 0.08 287 91 124 33 664.96 0.08 288 92 95 3 934.53 1.00 289 92 124 32 664.96 0.08 290 92 126 34 664.87 0.10 291 92 232 140 125.06 0.12 292 93 112 19 665.31 0.12 293 93 113 20 665.12 0.11 294 93 116 23 665.35 0.10 295 93 123 30 665.11 0.10 296 93 124 31 665.08 0.09 297 93 125 32 665.00 0.09 298 93 126 33 664.95 0.08 299 94 99 5 760.12 35.99 300 94 104 10 665.25 0.11 301 94 109 15 665.26 0.10 302 94 126 32 665.01 0.10 303 95 98 3 865.03 1.98 304 95 99 4 840.75 1.41 305 95 126 31 665.06 0.07 306 95 167 72 665.00 0.10 307 96 99 3 863.18 1.04 308 96 100 4 840.74 1.40 309 96 169 73 637.63 2.15 310 96 172 76 417.48 31.50 311 97 100 3 862.70 0.81 312 97 101 4 840.73 1.40 313 97 102 5 840.72 1.40 314 97 172 75 613.35 0.80 315 98 102 4 834.78 1.46 316 98 103 5 834.78 1.46 317 98 104 6 665.69 0.17 318 99 104 5 665.68 0.15 319 99 106 7 665.68 0.16 320 99 109 10 665.68 0.15 321 99 126 27 665.38 0.10 322 99 127 28 665.30 0.14 323 99 146 47 665.39 0.11 324 103 108 5 838.36 3.58 325 104 108 4 838.15 3.69 326 104 109 5 838.13 3.68 327 104 112 8 824.32 3.23 328 105 108 3 858.74 6.59 329 105 109 4 843.43 1.06 330 106 109 3 860.78 4.58 331 106 110 4 844.01 1.99 332 106 146 40 665.45 0.11 333 106 149 43 665.66 0.19 334 107 110 3 863.76 3.10 335 107 111 4 849.26 4.15 336 107 153 46 667.34 0.20 337 108 111 3 870.75 2.19 338 108 112 4 844.65 2.31 339 109 112 3 870.75 2.19 340 109 113 4 847.10 0.51 341 109 150 41 665.83 0.27 342 110 113 3 877.60 1.24 343 110 114 4 847.11 0.51 344 110 149 39 665.84 0.28 345 110 150 40 665.82 0.27 346 110 153 43 667.36 0.21 347 111 114 3 872.05 4.01 348 111 115 4 849.07 1.49 349 111 153 42 667.41 0.23 350 112 115 3 872.05 4.01 351 112 116 4 849.07 1.49 352 113 116 3 869.58 5.24 353 113 117 4 846.18 0.98 354 113 123 10 666.90 0.20 355 113 125 12 665.58 0.15 356 113 150 37 667.21 0.19 357 113 154 41 667.85 0.35 358 113 162 49 667.16 0.28 359 114 117 3 870.94 2.53 360 114 118 4 845.67 0.74 361 114 153 39 667.77 0.35 362 114 154 40 667.78 0.35 363 115 118 3 855.81 3.52 364 115 119 4 846.95 0.34 365 116 119 3 864.31 0.72 366 116 120 4 846.69 0.21 367 116 121 5 681.29 5.58 368 116 123 7 669.19 0.35 369 117 120 3 851.78 2.08 370 117 123 6 681.28 5.57 371 117 154 37 681.52 5.67 372 117 158 41 681.52 5.67 373 117 161 44 681.47 5.66 374 117 162 45 681.45 5.65 375 120 161 41 811.63 1.15 376 121 161 40 811.64 1.16 377 122 160 38 811.72 1.14 378 122 161 39 811.66 1.15 379 122 163 41 811.64 1.16 380 122 207 85 368.73 27.90 381 123 161 38 811.71 1.15 382 123 162 39 811.67 1.15 383 123 163 40 811.64 1.16 384 124 163 39 811.67 1.15 385 124 165 41 811.63 1.16 386 124 232 108 125.93 0.53 387 125 150 25 811.81 1.16 388 125 163 38 811.72 1.15 389 125 164 39 811.68 1.15 390 125 165 40 811.68 1.15 391 126 165 39 811.68 1.15 392 126 232 106 126.47 0.37 393 127 143 16 811.92 1.22 394 127 146 19 811.94 1.15 395 127 147 20 811.91 1.16 396 127 150 23 811.97 1.14 397 127 165 38 811.71 1.14 398 127 166 39 811.68 1.15 399 127 167 40 811.69 1.15 400 127 190 63 386.12 28.94 401 128 143 15 811.93 1.22 402 128 146 18 812.05 1.17 403 128 166 38 811.71 1.14 404 128 167 39 811.69 1.15 405 128 168 40 811.63 1.16 406 128 169 41 811.58 1.16 407 129 133 4 826.60 6.82 408 129 134 5 826.60 6.83 409 129 139 10 812.10 1.16 410 129 142 13 812.07 1.16 411 129 166 37 811.75 1.15 412 129 168 39 811.69 1.15 413 129 169 40 811.67 1.15 414 129 170 41 811.66 1.15 415 130 133 3 855.09 4.83 416 130 134 4 840.80 2.96 417 130 169 39 811.69 1.15 418 130 170 40 811.67 1.15 419 131 134 3 858.27 3.31 420 131 135 4 843.88 2.43 421 131 170 39 811.69 1.15 422 132 135 3 858.27 3.31 423 132 136 4 850.18 0.73 424 133 136 3 856.34 2.34 425 133 137 4 850.18 0.72 426 133 138 5 812.25 1.18 427 133 139 6 812.25 1.18 428 133 142 9 812.22 1.18 429 134 137 3 850.19 0.72 430 134 139 5 812.25 1.18 431 134 166 32 811.84 1.12 432 134 170 36 811.70 1.15 433 134 182 48 387.12 28.91 434 134 186 52 386.79 29.05 435 138 141 3 870.48 2.82 436 138 142 4 852.12 0.38 437 139 142 3 874.46 0.59 438 139 143 4 856.26 1.31 439 139 166 27 811.80 1.14 440 139 186 47 386.31 28.94 441 140 143 3 885.77 4.49 442 140 144 4 851.88 0.27 443 140 189 49 386.25 28.93 444 141 144 3 872.49 4.23 445 141 145 4 851.89 0.28 446 142 145 3 857.25 0.45 447 142 146 4 851.87 0.29 448 143 146 3 863.63 3.64 449 143 147 4 852.96 0.96 450 143 166 23 811.71 1.13 451 143 186 43 386.25 28.95 452 143 189 46 386.23 28.94 453 143 190 47 386.18 28.92 454 144 147 3 861.55 3.33 455 144 148 4 852.95 0.96 456 144 189 45 386.24 28.94 457 144 190 46 386.18 28.93 458 144 193 49 385.92 29.00 459 145 148 3 875.13 2.52 460 145 149 4 851.25 1.81 461 146 149 3 875.14 2.52 462 146 150 4 854.43 0.23 463 147 150 3 879.78 0.47 464 147 151 4 853.29 0.34 465 147 159 12 812.35 1.16 466 147 164 17 811.75 1.14 467 147 190 43 386.10 28.95 468 147 193 46 385.87 29.01 469 148 151 3 851.60 1.18 470 148 152 4 849.60 0.66 471 148 193 45 385.87 29.00 472 149 152 3 861.58 0.25 473 150 153 3 872.68 4.04 474 150 154 4 856.84 3.89 475 151 154 3 863.40 0.60 476 151 159 8 812.30 1.13 477 151 193 42 385.31 28.73 478 151 198 47 370.31 28.19 479 154 158 4 853.50 0.53 480 154 159 5 838.76 3.96 481 154 162 8 812.40 1.17 482 155 158 3 868.97 1.84 483 155 159 4 868.30 2.16 484 156 159 3 882.21 0.46 485 156 198 42 370.18 28.12 486 156 199 43 370.17 28.12 487 156 201 45 370.11 28.09 488 156 202 46 369.88 28.09 489 157 160 3 883.10 0.01 490 158 161 3 882.83 0.22 491 158 162 4 858.94 2.52 492 159 162 3 850.68 0.48 493 159 194 35 378.08 27.78 494 159 198 39 370.29 28.18 495 159 202 43 369.79 28.10 496 159 206 47 369.29 28.04 497 162 206 44 369.14 28.10 498 163 207 44 368.72 27.91 499 163 209 46 368.60 27.92 500 164 206 42 369.44 27.96 501 164 207 43 368.75 27.91 502 164 208 44 368.68 27.91 503 164 209 45 368.68 27.91 504 165 209 44 368.67 27.91 505 165 232 67 126.48 0.40 506 166 186 20 386.16 28.97 507 166 187 21 386.11 28.95 508 166 190 24 386.10 28.95 509 166 209 43 368.74 27.91 510 166 210 44 368.68 27.91 511 166 211 45 368.62 27.94 512 167 172 5 662.05 78.93 513 167 211 44 368.62 27.94 514 167 232 65 127.31 0.36 515 168 171 3 875.08 3.65 516 168 172 4 849.00 0.38 517 168 178 10 387.13 28.86 518 168 210 42 369.06 27.96 519 168 211 43 368.78 27.94 520 169 172 3 889.62 2.48 521 170 174 4 844.21 2.38 522 170 176 6 393.72 31.21 523 170 178 8 387.25 28.87 524 170 182 12 387.19 28.88 525 171 174 3 859.48 5.70 526 171 176 5 387.28 28.89 527 171 177 6 387.26 28.89 528 171 178 7 387.25 28.88 529 171 210 39 369.36 27.96 530 171 212 41 368.61 27.95 531 171 213 42 368.62 27.94 532 172 212 40 368.61 27.94 533 174 213 39 368.72 27.93 534 175 213 38 368.72 27.93 535 176 213 37 368.73 27.92 536 177 210 33 369.41 27.95 537 177 212 35 368.80 27.89 538 177 213 36 368.75 27.91 539 177 218 41 336.76 31.63 540 177 222 45 229.65 2.83 541 178 182 4 858.67 3.97 542 178 183 5 817.12 1.24 543 178 210 32 369.41 27.95 544 178 222 44 140.99 0.46 545 179 182 3 868.06 0.71 546 179 183 4 858.84 3.90 547 180 183 3 868.08 0.71 548 180 184 4 858.85 3.91 549 180 221 41 140.07 0.26 550 180 222 42 140.00 0.24 551 180 225 45 138.91 0.73 552 181 184 3 867.87 0.48 553 181 185 4 859.09 3.79 554 181 225 44 138.96 0.70 555 182 185 3 874.95 3.85 556 182 186 4 874.98 3.51 557 183 186 3 881.98 4.49 558 183 187 4 867.33 0.32 559 183 210 27 369.46 27.96 560 183 222 39 140.00 0.24 561 183 228 45 128.46 0.62 562 184 187 3 876.12 2.94 563 184 188 4 868.21 0.76 564 184 225 41 128.85 0.83 565 184 226 42 128.89 0.83 566 184 227 43 128.47 0.63 567 184 228 44 128.45 0.62 568 185 188 3 876.11 2.94 569 185 189 4 868.23 0.76 570 186 189 3 868.46 0.87 571 186 190 4 868.23 0.76 572 187 190 3 862.52 3.62 573 187 191 4 867.58 1.08 574 187 208 21 369.62 27.95 575 187 209 22 369.61 27.95 576 187 210 23 369.49 27.98 577 187 227 40 128.18 0.58 578 187 228 41 128.06 0.55 579 188 191 3 867.58 1.08 580 188 192 4 867.56 1.07 581 188 227 39 128.19 0.58 582 189 192 3 870.53 0.27 583 189 193 4 867.52 1.05 584 190 193 3 868.25 1.42 585 190 194 4 862.36 3.63 586 190 208 18 369.63 27.95 587 191 194 3 868.96 1.25 588 191 195 4 862.09 3.75 589 191 203 12 370.37 28.20 590 191 206 15 370.00 28.11 591 191 208 17 369.63 27.94 592 191 227 36 128.09 0.56 593 191 229 38 128.05 0.58 594 192 195 3 862.10 3.75 595 192 196 4 862.11 3.75 596 193 196 3 863.80 3.84 597 193 197 4 862.08 3.75 598 193 198 5 862.10 3.75 599 194 197 3 867.02 1.28 600 194 198 4 860.77 4.41 601 194 199 5 843.25 4.36 602 194 202 8 410.64 27.50 603 194 203 9 370.39 28.20 604 194 206 12 369.98 28.11 605 195 199 4 815.30 1.79 606 195 200 5 424.07 16.27 607 195 203 8 370.46 28.19 608 198 202 4 853.52 0.33 609 199 202 3 867.28 0.67 610 199 203 4 856.03 1.30 611 200 203 3 867.27 0.68 612 200 204 4 857.76 0.43 613 201 204 3 878.27 5.73 614 201 205 4 860.81 1.96 615 202 205 3 888.21 2.25 616 202 206 4 855.07 1.77 617 203 206 3 861.09 1.81 618 206 229 23 127.63 0.45 619 207 229 22 127.46 0.36 620 208 227 19 128.00 0.57 621 208 228 20 127.76 0.49 622 208 229 21 127.64 0.45 623 209 229 20 127.64 0.45 624 209 230 21 127.40 0.38 625 209 231 22 127.34 0.36 626 209 232 23 127.33 0.35 627 210 214 4 877.50 6.16 628 210 222 12 131.93 1.03 629 210 228 18 127.87 0.48 630 210 230 20 127.66 0.46 631 210 231 21 127.39 0.38 632 210 232 22 127.39 0.38 633 211 214 3 889.78 3.43 634 211 232 21 127.32 0.36 635 213 218 5 851.82 0.94 636 214 218 4 832.99 6.69 637 214 219 5 393.75 26.57 638 214 222 8 187.72 23.79 639 214 231 17 127.71 0.48 640 214 233 19 127.56 0.44 641 214 236 22 127.27 0.38 642 214 245 31 52.26 0.17 643 215 218 3 870.86 2.85 644 215 219 4 602.75 46.14 645 215 236 21 127.31 0.39 646 215 245 30 52.34 0.16 647 216 241 25 53.26 0.42 648 216 244 28 53.23 0.41 649 216 245 29 52.29 0.15 650 218 221 3 881.88 3.23 651 218 222 4 855.32 0.29 652 219 222 3 866.82 0.01 653 219 223 4 856.90 1.57 654 219 231 12 128.38 0.59 655 219 245 26 52.12 0.17 656 219 248 29 37.41 1.32 657 220 223 3 867.82 0.48 658 220 224 4 856.91 1.57 659 220 248 28 37.34 1.35 660 221 224 3 867.81 0.48 661 222 225 3 876.23 0.83 662 222 228 6 129.00 0.83 663 222 231 9 128.88 0.81 664 223 228 5 129.07 0.76 665 223 229 6 128.96 0.82 666 223 230 7 128.93 0.82 667 223 231 8 128.93 0.82 668 223 248 25 37.34 1.35 669 223 249 26 30.75 3.08 670 225 228 3 877.62 0.17 671 228 231 3 1061.66 3.66 672 230 249 19 20.61 0.14 673 231 245 14 50.19 0.47 674 231 249 18 26.23 0.07 675 233 236 3 894.23 1.59 676 233 237 4 822.16 1.53 677 233 242 9 55.41 0.58 678 233 245 12 52.12 0.20 679 234 237 3 887.20 1.60 680 235 238 3 887.20 1.60 681 235 239 4 878.28 2.86 682 236 239 3 876.18 1.81 683 236 242 6 180.16 23.63 684 236 245 9 53.93 0.11 685 237 242 5 136.31 3.31 686 239 242 3 881.94 2.46 687 240 243 3 887.67 5.46 688 241 244 3 868.74 2.26 689 241 245 4 854.22 0.62 690 242 245 3 872.74 5.71 691 242 246 4 849.24 1.87 692 243 246 3 871.96 6.10 693 243 247 4 851.12 0.93 694 244 247 3 851.84 0.57 695 244 248 4 851.04 0.88 696 245 248 3 856.93 1.42 697 246 249 3 885.35 2.69 698 247 250 3 883.42 1.73