BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 17 15 32.68 0.61 2 2 20 18 28.79 0.01 3 2 21 19 25.56 0.68 4 2 24 22 24.23 0.04 5 2 49 47 25.89 0.72 6 2 50 48 25.68 0.62 7 2 51 49 32.48 0.10 8 3 17 14 33.24 0.45 9 3 51 48 32.48 0.10 10 4 9 5 793.76 5.20 11 4 13 9 47.70 3.19 12 4 14 10 37.30 0.52 13 4 17 13 33.30 0.47 14 4 51 47 33.26 0.45 15 4 52 48 35.86 0.85 16 4 53 49 58.41 0.52 17 4 129 125 64.50 0.64 18 4 132 128 64.34 0.72 19 5 9 4 802.22 6.11 20 5 53 48 60.75 0.79 21 5 57 52 65.58 0.18 22 6 9 3 826.87 0.12 23 6 53 47 64.48 0.66 24 6 54 48 65.02 0.39 25 6 55 49 66.73 0.21 26 6 129 123 64.59 0.68 27 6 132 126 64.95 0.50 28 6 133 127 65.11 0.42 29 6 136 130 65.58 0.19 30 7 55 48 71.15 1.97 31 9 13 4 828.76 5.66 32 9 129 120 82.02 0.10 33 9 175 166 86.95 0.05 34 10 13 3 842.30 0.18 35 10 14 4 843.25 0.28 36 10 175 165 92.73 0.19 37 11 14 3 862.94 3.12 38 11 15 4 843.25 0.28 39 11 172 161 84.09 0.76 40 11 173 162 89.61 1.33 41 11 175 164 93.56 0.17 42 12 15 3 846.12 0.01 43 12 16 4 843.26 0.27 44 13 16 3 843.26 0.27 45 13 17 4 837.52 2.60 46 14 17 3 854.23 5.75 47 14 18 4 837.52 2.60 48 14 129 115 101.32 0.60 49 14 157 143 97.82 0.12 50 14 161 147 93.56 0.17 51 14 168 154 93.58 0.17 52 14 171 157 99.64 0.57 53 14 172 158 97.60 0.46 54 15 18 3 854.27 5.72 55 15 19 4 837.56 2.64 56 15 168 153 97.25 0.29 57 16 19 3 856.74 4.49 58 16 20 4 835.45 3.69 59 17 20 3 856.75 4.48 60 17 21 4 844.47 0.82 61 17 50 33 107.03 0.66 62 17 52 35 124.79 0.18 63 17 158 141 101.37 0.58 64 18 21 3 844.68 0.74 65 18 22 4 844.47 0.83 66 18 157 139 105.28 0.21 67 18 158 140 101.55 0.50 68 18 161 143 101.34 0.57 69 18 162 144 100.48 0.15 70 18 168 150 101.37 0.59 71 19 22 3 847.24 2.02 72 19 23 4 847.03 2.12 73 20 23 3 848.41 1.44 74 20 24 4 846.87 2.21 75 21 24 3 844.58 3.35 76 21 25 4 840.11 5.58 77 21 50 29 125.42 0.20 78 21 64 43 124.88 0.43 79 21 158 137 125.23 0.29 80 21 162 141 120.24 0.42 81 22 25 3 849.17 1.05 82 22 161 139 125.26 0.28 83 22 162 140 122.34 0.61 84 22 164 142 124.62 0.30 85 22 165 143 125.23 0.29 86 22 168 146 125.33 0.26 87 22 238 216 111.61 0.77 88 22 239 217 107.09 0.62 89 25 37 12 125.51 0.18 90 25 64 39 126.09 0.25 91 25 66 41 125.51 0.18 92 25 69 44 125.59 0.12 93 25 239 214 125.50 0.18 94 26 37 11 126.00 0.42 95 26 38 12 126.02 0.42 96 26 41 15 126.14 0.35 97 26 66 40 126.10 0.27 98 27 35 8 126.16 0.33 99 27 36 9 125.99 0.42 100 27 37 10 126.00 0.42 101 27 38 11 126.05 0.43 102 28 36 8 126.23 0.33 103 28 37 9 126.23 0.34 104 28 38 10 126.14 0.40 105 28 39 11 126.37 0.41 106 28 242 214 125.64 0.10 107 29 35 6 126.45 0.35 108 29 36 7 126.30 0.34 109 30 33 3 842.61 1.19 110 30 34 4 732.36 4.94 111 30 35 5 126.59 0.29 112 31 34 3 838.31 3.33 113 31 36 5 732.08 5.08 114 31 200 169 133.32 3.19 115 31 203 172 141.03 7.24 116 31 240 209 126.80 0.22 117 32 199 167 141.55 7.22 118 32 202 170 142.38 7.63 119 32 203 171 142.37 7.63 120 32 207 175 142.39 7.65 121 32 228 196 142.28 7.70 122 34 199 165 150.83 3.44 123 34 233 199 150.99 3.47 124 34 237 203 151.03 3.45 125 34 238 204 150.87 3.37 126 34 239 205 150.85 3.38 127 34 240 206 150.84 3.38 128 35 238 203 151.09 3.46 129 35 239 204 150.88 3.38 130 35 240 205 150.87 3.37 131 36 200 164 151.00 3.46 132 36 240 204 150.87 3.37 133 36 242 206 150.84 3.38 134 37 66 29 151.46 3.29 135 37 67 30 151.44 3.29 136 37 68 31 151.44 3.29 137 37 69 32 381.85 118.49 138 37 192 155 151.22 3.42 139 37 239 202 151.18 3.44 140 37 240 203 151.05 3.43 141 37 242 205 150.84 3.38 142 38 41 3 915.39 2.56 143 38 67 29 396.34 125.73 144 38 242 204 150.88 3.37 145 38 245 207 149.32 2.71 146 39 242 203 150.91 3.37 147 39 244 205 150.78 3.35 148 39 245 206 148.66 2.39 149 40 67 27 676.71 19.39 150 41 45 4 812.50 6.21 151 41 46 5 732.63 3.87 152 41 66 25 682.62 21.55 153 41 67 26 684.90 15.30 154 42 45 3 843.28 0.02 155 42 46 4 806.78 9.07 156 43 46 3 832.72 3.13 157 43 47 4 832.72 3.13 158 43 94 51 634.83 6.59 159 44 94 50 635.53 6.45 160 45 66 21 728.44 4.11 161 46 65 19 728.46 4.11 162 46 66 20 728.44 4.11 163 46 67 21 728.43 4.11 164 46 90 44 680.38 3.06 165 47 64 17 728.51 4.12 166 47 65 18 728.48 4.11 167 47 66 19 728.48 4.12 168 47 72 25 727.13 3.73 169 47 90 43 680.61 2.98 170 47 91 44 680.38 3.07 171 47 94 47 680.34 3.08 172 47 95 48 679.91 3.23 173 48 63 15 728.58 4.10 174 48 64 16 728.57 4.11 175 48 66 18 728.50 4.12 176 49 62 13 728.64 4.09 177 49 63 14 728.58 4.10 178 49 95 46 680.64 2.96 179 49 96 47 680.59 2.96 180 50 61 11 728.72 4.09 181 50 62 12 728.67 4.09 182 50 64 14 728.65 4.09 183 50 158 108 455.51 0.18 184 51 59 8 729.03 4.07 185 51 60 9 729.00 4.08 186 51 61 10 728.79 4.05 187 52 59 7 729.03 4.07 188 52 60 8 729.01 4.07 189 52 62 10 728.93 4.10 190 52 132 80 549.71 0.78 191 52 136 84 551.18 0.01 192 52 154 102 512.92 0.84 193 52 158 106 474.35 0.68 194 53 57 4 733.77 5.79 195 53 58 5 729.14 4.09 196 53 59 6 729.11 4.11 197 53 136 83 551.26 0.01 198 54 57 3 839.53 10.85 199 54 58 4 729.92 3.71 200 54 133 79 550.81 0.23 201 54 136 82 551.35 0.04 202 54 137 83 551.26 0.01 203 55 130 75 551.27 0.04 204 55 133 78 551.27 0.01 205 58 136 78 551.34 0.04 206 58 137 79 551.28 0.07 207 58 140 82 551.17 0.18 208 58 141 83 551.19 0.19 209 59 140 81 551.12 0.20 210 60 74 14 727.48 4.00 211 60 75 15 726.91 3.80 212 60 76 16 726.79 3.78 213 60 136 76 550.89 0.14 214 60 139 79 550.97 0.18 215 60 140 80 550.96 0.18 216 60 154 94 512.97 0.86 217 61 74 13 727.01 3.77 218 61 75 14 726.87 3.79 219 61 96 35 680.71 2.97 220 62 71 9 727.85 4.09 221 62 73 11 727.12 3.75 222 62 74 12 726.88 3.81 223 62 96 34 680.70 2.96 224 62 154 92 512.28 0.56 225 63 71 8 727.68 4.00 226 63 72 9 727.49 3.92 227 63 73 10 727.13 3.75 228 63 95 32 680.63 2.98 229 63 96 33 680.62 2.98 230 64 69 5 728.36 4.13 231 64 70 6 727.69 4.00 232 64 71 7 727.34 3.83 233 65 68 3 865.58 4.84 234 65 69 4 729.57 4.28 235 65 70 5 728.13 4.21 236 65 72 7 727.14 3.73 237 67 241 174 166.50 4.17 238 67 245 178 143.60 5.84 239 68 163 95 438.54 1.73 240 68 192 124 218.44 2.58 241 68 241 173 168.03 3.41 242 69 159 90 445.82 1.40 243 69 162 93 438.57 1.72 244 69 163 94 438.55 1.72 245 69 192 123 221.34 1.16 246 69 239 170 191.22 2.58 247 70 163 93 438.60 1.71 248 71 83 12 681.56 3.10 249 71 154 83 512.00 0.50 250 71 155 84 497.10 1.26 251 71 158 87 455.45 0.21 252 71 159 88 452.65 0.91 253 72 82 10 681.54 3.12 254 72 83 11 681.45 3.16 255 72 87 15 681.21 3.13 256 72 88 16 680.86 3.00 257 72 91 19 680.76 3.01 258 73 81 8 681.55 3.12 259 73 82 9 681.54 3.12 260 74 80 6 681.85 3.12 261 74 81 7 681.71 3.06 262 74 96 22 680.81 2.97 263 74 139 65 550.83 0.24 264 74 142 68 550.36 0.47 265 74 151 77 540.86 0.89 266 75 79 4 769.85 14.09 267 75 80 5 681.86 3.12 268 75 96 21 680.81 2.97 269 75 98 23 680.78 2.96 270 75 99 24 680.49 3.02 271 76 79 3 854.56 5.07 272 76 81 5 740.59 0.56 273 76 140 64 550.79 0.26 274 78 99 21 680.54 3.02 275 78 100 22 680.52 3.01 276 78 101 23 679.86 3.34 277 79 99 20 680.56 3.02 278 79 101 22 679.75 3.37 279 79 103 24 675.20 1.19 280 80 96 16 680.87 2.97 281 80 99 19 680.52 3.02 282 80 101 21 679.70 3.40 283 80 102 22 657.06 7.75 284 80 103 23 623.83 9.81 285 81 103 22 568.45 6.33 286 81 105 24 567.84 6.61 287 81 142 61 550.30 0.48 288 82 88 6 681.48 3.02 289 82 91 9 681.47 3.02 290 82 92 10 681.41 3.02 291 82 95 13 681.28 3.08 292 82 96 14 681.01 3.04 293 82 102 20 636.19 3.22 294 82 103 21 568.45 6.33 295 82 104 22 557.64 1.61 296 82 105 23 557.59 1.59 297 83 88 5 756.15 17.45 298 83 105 22 557.56 1.59 299 83 107 24 555.06 1.26 300 83 108 25 552.85 2.35 301 83 109 26 552.84 2.34 302 83 114 31 552.83 2.33 303 83 151 68 541.26 0.96 304 83 155 72 513.03 0.87 305 84 104 20 567.81 6.62 306 84 105 21 557.64 1.63 307 84 107 23 552.88 2.37 308 84 108 24 552.87 2.35 309 85 88 3 845.47 5.41 310 85 89 4 830.10 2.23 311 85 108 23 552.84 2.36 312 85 109 24 552.85 2.35 313 86 89 3 845.47 5.41 314 86 90 4 830.25 2.15 315 87 90 3 841.75 3.69 316 87 91 4 836.78 1.23 317 88 91 3 840.56 1.89 318 88 92 4 836.93 1.16 319 89 92 3 851.74 3.81 320 89 93 4 856.16 1.59 321 90 93 3 856.63 1.36 322 90 94 4 856.18 1.59 323 91 94 3 856.64 1.36 324 91 95 4 846.38 4.94 325 92 95 3 856.18 1.59 326 92 97 5 827.76 3.36 327 92 102 10 618.53 11.62 328 93 97 4 846.63 4.81 329 94 97 3 887.35 0.47 330 96 99 3 857.13 3.68 331 105 142 37 551.00 0.38 332 105 151 46 549.46 0.83 333 107 113 6 760.14 0.97 334 107 114 7 740.02 6.66 335 107 117 10 556.21 0.61 336 107 147 40 550.70 0.55 337 107 148 41 550.48 0.65 338 107 151 44 550.17 0.77 339 108 113 5 807.57 4.75 340 108 114 6 807.59 4.74 341 109 114 5 817.85 0.36 342 110 113 3 857.95 2.00 343 110 114 4 826.73 3.96 344 111 114 3 842.36 0.91 345 111 115 4 826.72 3.96 346 111 122 11 552.44 0.18 347 111 152 41 550.04 0.83 348 111 155 44 549.66 0.92 349 111 156 45 549.46 0.83 350 111 160 49 506.44 1.15 351 111 189 78 302.11 1.89 352 112 115 3 843.83 1.62 353 112 116 4 831.29 4.63 354 113 116 3 836.85 5.70 355 113 117 4 828.34 3.17 356 114 117 3 846.24 1.01 357 114 118 4 822.97 2.17 358 114 148 34 550.63 0.66 359 114 151 37 550.51 0.70 360 114 152 38 550.09 0.85 361 114 155 41 549.49 0.83 362 115 118 3 843.14 2.56 363 115 119 4 818.22 0.24 364 115 120 5 765.65 13.98 365 115 121 6 558.18 2.10 366 115 122 7 558.20 2.12 367 115 152 37 550.25 0.94 368 116 119 3 829.10 5.28 369 117 148 31 550.75 0.71 370 118 148 30 550.75 0.71 371 118 149 31 550.75 0.71 372 118 152 34 550.68 0.75 373 120 124 4 830.80 6.54 374 120 125 5 801.89 1.08 375 121 124 3 855.52 3.47 376 121 125 4 840.91 1.48 377 122 125 3 841.62 1.13 378 122 152 30 549.87 1.17 379 122 156 34 548.68 1.56 380 122 181 59 425.75 2.81 381 122 186 64 387.95 0.82 382 122 189 67 355.54 5.45 383 122 190 68 348.93 2.12 384 123 180 57 426.69 3.07 385 123 181 58 425.86 2.77 386 123 183 60 422.74 2.48 387 123 186 63 393.05 3.29 388 124 180 56 426.88 3.10 389 125 149 24 549.93 1.22 390 125 152 27 549.89 1.24 391 125 153 28 548.36 2.00 392 125 180 55 426.72 3.06 393 125 181 56 425.92 2.79 394 126 131 5 806.87 1.79 395 126 153 27 548.33 2.04 396 126 178 52 428.27 2.69 397 126 179 53 428.13 2.68 398 126 180 54 426.69 3.07 399 127 131 4 820.60 0.92 400 127 153 26 548.20 2.12 401 127 156 29 547.91 2.25 402 127 171 44 435.99 1.07 403 127 178 51 428.25 2.71 404 127 179 52 428.12 2.68 405 127 181 54 425.50 2.71 406 128 131 3 844.84 6.15 407 128 132 4 847.16 3.50 408 128 171 43 435.93 1.04 409 128 174 46 434.26 1.06 410 128 178 50 428.26 2.71 411 128 179 51 428.25 2.71 412 129 132 3 850.98 1.59 413 129 133 4 847.16 3.50 414 129 174 45 434.26 1.07 415 129 175 46 433.63 0.79 416 130 133 3 847.16 3.50 417 130 134 4 847.19 3.48 418 130 174 44 434.43 1.14 419 130 175 45 434.25 1.06 420 130 176 46 431.93 1.05 421 130 177 47 431.72 1.14 422 130 178 48 428.29 2.69 423 131 134 3 849.12 4.41 424 131 135 4 845.97 2.88 425 131 153 22 548.06 2.25 426 131 178 47 428.25 2.71 427 132 135 3 845.96 2.89 428 132 136 4 845.93 2.90 429 132 153 21 548.00 2.28 430 132 154 22 547.84 2.35 431 132 157 25 533.47 9.47 432 133 136 3 859.43 3.51 433 133 137 4 842.41 4.66 434 134 137 3 849.09 1.32 435 134 138 4 839.73 6.00 436 135 138 3 856.75 2.52 437 135 139 4 839.70 6.00 438 135 150 15 548.30 2.14 439 135 153 18 547.84 2.37 440 136 139 3 849.81 0.96 441 136 140 4 839.68 6.01 442 137 140 3 846.65 2.48 443 137 141 4 837.83 6.90 444 138 141 3 846.14 2.22 445 138 142 4 814.91 0.46 446 138 143 5 810.40 2.71 447 138 146 8 548.98 2.04 448 138 147 9 548.59 2.18 449 138 150 12 548.45 2.21 450 139 142 3 819.29 2.37 451 139 147 8 548.51 2.21 452 139 150 11 548.30 2.29 453 139 151 12 548.28 2.30 454 139 154 15 541.46 5.71 455 142 147 5 762.13 12.50 456 143 146 3 857.40 2.75 457 143 147 4 797.57 5.21 458 144 147 3 843.50 7.48 459 146 149 3 878.25 0.59 460 146 150 4 829.23 2.67 461 147 150 3 847.64 1.44 462 147 151 4 829.23 2.67 463 148 151 3 835.95 0.69 464 148 152 4 829.25 2.67 465 149 152 3 843.71 2.41 466 149 153 4 830.68 1.95 467 150 153 3 848.47 0.02 468 150 154 4 830.66 1.96 469 151 154 3 825.69 0.55 470 151 155 4 825.02 0.86 471 152 155 3 836.81 1.51 472 152 156 4 821.73 0.79 473 153 156 3 841.88 0.81 474 153 157 4 821.15 0.96 475 154 157 3 843.98 1.86 476 154 158 4 831.46 4.20 477 155 158 3 848.01 0.16 478 155 159 4 814.94 2.48 479 155 160 5 787.68 6.64 480 156 160 4 784.68 5.13 481 156 161 5 804.68 4.87 482 156 167 11 437.37 1.66 483 156 171 15 435.95 1.05 484 156 189 33 269.25 14.41 485 157 161 4 806.08 9.22 486 157 171 14 435.86 1.10 487 158 161 3 843.77 4.92 488 158 162 4 826.59 1.03 489 159 162 3 839.93 6.84 490 159 163 4 832.24 3.86 491 160 163 3 848.98 6.98 492 160 164 4 826.58 1.03 493 160 167 7 677.11 43.26 494 160 189 29 266.78 15.49 495 161 164 3 835.04 0.40 496 161 167 6 511.36 38.65 497 161 168 7 437.07 1.72 498 162 238 76 191.38 2.54 499 162 239 77 191.34 2.53 500 163 192 29 227.98 3.71 501 163 237 74 191.97 2.65 502 163 238 75 191.42 2.52 503 163 239 76 191.23 2.58 504 164 167 3 835.58 2.13 505 164 168 4 828.00 0.55 506 164 188 24 302.02 1.90 507 164 192 28 239.51 1.80 508 164 195 31 261.58 12.86 509 164 236 72 191.99 2.64 510 164 237 73 191.99 2.64 511 165 168 3 841.00 1.82 512 165 169 4 829.32 0.43 513 165 236 71 192.00 2.64 514 165 238 73 191.82 2.73 515 166 169 3 866.74 3.45 516 166 170 4 832.34 1.08 517 166 184 18 368.48 1.21 518 166 185 19 359.78 3.15 519 166 188 22 321.24 5.04 520 166 236 70 192.37 2.82 521 167 170 3 859.55 1.71 522 167 171 4 829.37 0.41 523 167 185 18 351.22 1.13 524 167 188 21 314.81 8.25 525 167 189 22 314.81 8.25 526 168 171 3 859.27 2.75 527 168 172 4 829.22 0.40 528 169 172 3 843.07 5.34 529 169 173 4 840.69 5.33 530 170 173 3 859.00 0.04 531 170 174 4 852.21 0.42 532 170 179 9 428.19 2.73 533 170 185 15 372.64 1.27 534 171 174 3 861.16 1.03 535 171 179 8 428.22 2.70 536 172 175 3 890.51 5.71 537 174 177 3 843.06 6.48 538 174 178 4 775.24 4.01 539 174 179 5 755.43 5.41 540 179 185 6 842.58 3.06 541 181 185 4 832.89 1.79 542 181 186 5 822.59 3.40 543 182 185 3 866.44 5.62 544 182 186 4 842.58 3.06 545 183 186 3 846.95 0.87 546 183 187 4 832.25 8.21 547 183 216 33 280.81 0.49 548 183 220 37 280.86 0.55 549 184 187 3 848.22 5.87 550 184 188 4 842.58 3.06 551 184 216 32 280.82 0.49 552 184 217 33 281.29 0.35 553 184 218 34 281.45 0.41 554 185 188 3 846.94 0.86 555 185 189 4 822.65 3.42 556 186 189 3 843.44 2.88 557 186 190 4 820.66 4.41 558 187 190 3 837.63 4.08 559 187 191 4 819.33 3.74 560 187 215 28 281.33 0.42 561 187 216 29 281.63 0.31 562 188 191 3 860.31 6.12 563 188 192 4 820.03 4.49 564 188 215 27 281.72 0.28 565 188 232 44 200.37 0.15 566 188 236 48 200.01 0.33 567 189 192 3 873.44 0.45 568 191 194 3 845.48 6.11 569 191 195 4 827.64 1.58 570 191 213 22 281.80 0.27 571 191 215 24 281.85 0.29 572 192 195 3 848.37 0.12 573 192 196 4 829.55 0.64 574 192 237 45 199.32 0.68 575 192 239 47 199.28 0.67 576 192 241 49 197.32 0.46 577 193 196 3 840.65 3.97 578 193 197 4 829.56 0.65 579 193 241 48 197.28 0.48 580 193 246 53 164.73 3.62 581 194 197 3 834.25 1.45 582 194 198 4 829.50 0.62 583 194 213 19 288.05 1.79 584 195 198 3 854.61 4.19 585 195 199 4 840.26 1.55 586 195 209 14 289.43 1.20 587 195 213 18 288.06 1.79 588 195 215 20 288.07 1.78 589 195 225 30 288.04 1.79 590 195 232 37 209.12 1.58 591 195 237 42 199.44 0.61 592 196 199 3 854.37 4.75 593 196 200 4 840.27 1.55 594 196 240 44 198.66 0.35 595 196 241 45 197.55 0.35 596 196 246 50 179.99 5.08 597 197 200 3 840.27 1.55 598 197 201 4 833.97 1.60 599 197 246 49 181.23 4.47 600 198 201 3 833.96 1.60 601 198 202 4 833.94 1.62 602 198 209 11 289.51 1.25 603 198 210 12 289.72 1.35 604 198 211 13 289.69 1.36 605 198 213 15 289.31 1.53 606 199 202 3 833.00 2.09 607 199 203 4 832.89 2.14 608 199 209 10 289.74 1.33 609 199 233 34 220.56 6.59 610 199 237 38 201.37 0.99 611 199 240 41 199.44 0.70 612 200 203 3 858.00 0.91 613 200 204 4 849.80 3.20 614 200 241 41 199.47 0.70 615 200 242 42 199.40 0.68 616 200 243 43 198.64 0.37 617 200 246 46 198.48 0.23 618 201 204 3 844.58 3.70 619 201 205 4 832.57 2.06 620 201 210 9 289.77 1.32 621 202 205 3 832.58 2.06 622 202 206 4 832.57 2.06 623 202 207 5 292.38 2.12 624 202 208 6 290.25 1.08 625 202 209 7 289.79 1.32 626 202 228 26 288.25 1.97 627 205 208 3 840.61 7.55 628 207 227 20 288.46 1.95 629 207 228 21 288.34 1.98 630 208 226 18 288.55 1.97 631 208 227 19 288.36 2.00 632 209 213 4 864.04 4.00 633 209 225 16 288.59 1.98 634 209 226 17 288.38 2.01 635 209 227 18 288.36 2.00 636 209 228 19 288.36 2.00 637 209 233 24 263.33 14.51 638 212 224 12 289.06 2.08 639 212 225 13 288.67 1.96 640 212 226 14 288.49 1.97 641 213 224 11 289.33 2.21 642 213 225 12 288.78 2.00 643 214 222 8 289.95 2.21 644 214 223 9 289.84 2.26 645 214 224 10 289.36 2.21 646 215 222 7 290.14 2.28 647 215 223 8 289.87 2.25 648 215 225 10 289.80 2.24 649 215 232 17 287.12 2.58 650 216 220 4 321.77 0.27 651 216 221 5 298.50 1.83 652 216 222 6 290.15 2.28 653 217 220 3 834.18 1.38 654 217 221 4 312.66 4.29 655 217 223 6 290.43 2.21 656 225 230 5 314.23 2.02 657 225 232 7 529.85 105.83 658 227 230 3 875.83 0.26 659 227 233 6 541.53 100.39 660 228 233 5 541.53 100.39 661 228 234 6 541.53 100.39 662 229 234 5 684.02 29.27 663 230 233 3 853.68 1.02 664 230 234 4 810.02 7.52 665 231 234 3 841.79 6.96 666 231 235 4 775.83 3.87 667 231 236 5 755.31 6.40 668 232 236 4 755.31 6.40 669 232 237 5 755.31 6.40 670 233 237 4 755.31 6.40 671 237 240 3 936.98 3.17 672 241 245 4 817.46 7.71 673 241 246 5 805.92 11.71 674 242 245 3 839.07 3.10 675 242 246 4 839.48 2.89 676 243 246 3 866.46 4.50