BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 27 26 596.51 0.58 2 1 47 46 542.97 1.48 3 1 48 47 542.97 1.48 4 1 225 224 80.39 0.26 5 1 226 225 80.58 0.15 6 1 228 227 77.04 0.45 7 2 27 25 596.51 0.58 8 2 29 27 595.89 0.39 9 2 44 42 543.19 1.47 10 2 45 43 543.14 1.47 11 2 46 44 543.00 1.47 12 2 47 45 542.99 1.47 13 2 48 46 542.97 1.48 14 3 20 17 596.86 0.60 15 3 26 23 596.81 0.53 16 3 27 24 596.77 0.52 17 3 28 25 596.55 0.57 18 3 29 26 596.53 0.56 19 3 30 27 596.49 0.57 20 3 46 43 543.10 1.46 21 3 48 45 542.99 1.48 22 3 50 47 542.97 1.48 23 3 225 222 82.24 0.09 24 4 28 24 596.73 0.54 25 4 30 26 596.43 0.59 26 4 41 37 543.15 1.48 27 4 46 42 543.18 1.48 28 4 48 44 543.05 1.47 29 4 49 45 542.98 1.48 30 4 50 46 542.98 1.48 31 5 8 3 838.47 2.16 32 5 13 8 597.69 0.68 33 5 16 11 597.24 0.58 34 5 17 12 597.15 0.63 35 5 20 15 597.22 0.57 36 5 28 23 596.82 0.52 37 5 30 25 596.61 0.58 38 5 31 26 596.49 0.57 39 5 32 27 596.48 0.57 40 5 50 45 542.99 1.48 41 6 32 26 596.49 0.57 42 6 38 32 543.49 1.41 43 6 49 43 543.11 1.46 44 6 50 44 543.01 1.48 45 6 51 45 542.97 1.48 46 6 52 46 542.95 1.49 47 6 53 47 542.78 1.52 48 6 61 55 458.88 1.30 49 6 65 59 458.67 1.23 50 7 10 3 836.55 0.49 51 7 13 6 597.74 0.69 52 7 32 25 596.57 0.58 53 7 33 26 596.41 0.59 54 7 52 45 542.97 1.48 55 7 53 46 542.76 1.54 56 8 13 5 597.79 0.68 57 8 14 6 597.75 0.70 58 8 17 9 597.71 0.69 59 8 31 23 596.77 0.51 60 8 32 24 596.76 0.51 61 8 33 25 596.56 0.58 62 9 33 24 596.73 0.50 63 10 13 3 827.18 1.12 64 10 14 4 822.32 3.44 65 10 33 23 596.73 0.50 66 11 14 3 861.37 3.52 67 11 15 4 842.78 3.88 68 11 133 122 102.69 0.05 69 11 135 124 105.06 1.12 70 11 214 203 95.67 1.59 71 12 15 3 845.58 2.68 72 12 16 4 842.19 4.17 73 12 52 40 543.00 1.46 74 12 214 202 95.44 1.50 75 13 16 3 842.19 4.17 76 13 17 4 833.60 0.67 77 13 52 39 542.98 1.47 78 14 17 3 837.43 1.24 79 14 18 4 833.60 0.67 80 15 18 3 841.64 0.87 81 15 19 4 833.60 0.67 82 15 214 199 95.42 1.51 83 15 215 200 95.27 1.55 84 15 218 203 94.31 1.99 85 16 19 3 837.41 1.24 86 16 20 4 833.36 0.79 87 16 50 34 543.07 1.45 88 16 52 36 542.99 1.48 89 16 218 202 94.29 1.99 90 16 221 205 94.23 1.99 91 16 222 206 94.20 1.99 92 17 20 3 856.46 2.95 93 17 21 4 818.98 6.41 94 17 28 11 596.92 0.55 95 17 31 14 596.80 0.52 96 18 21 3 852.34 0.90 97 18 22 4 818.98 6.42 98 19 22 3 842.02 0.69 99 19 23 4 827.59 2.41 100 19 218 199 94.27 1.98 101 19 222 203 94.20 1.99 102 20 23 3 834.09 0.84 103 20 26 6 597.46 0.34 104 20 27 7 597.44 0.35 105 20 28 8 596.91 0.55 106 20 50 30 543.00 1.48 107 20 222 202 94.17 1.98 108 21 28 7 596.93 0.54 109 23 26 3 833.85 5.00 110 23 222 199 94.19 1.98 111 23 226 203 82.86 0.39 112 25 226 201 82.24 0.12 113 26 222 196 93.76 1.79 114 26 225 199 82.28 0.10 115 26 226 200 82.20 0.10 116 28 31 3 968.94 1.19 117 29 44 15 543.19 1.48 118 29 46 17 543.00 1.47 119 30 41 11 543.13 1.50 120 30 46 16 543.14 1.50 121 32 36 4 609.23 4.82 122 32 37 5 609.23 4.82 123 32 38 6 570.07 14.73 124 32 40 8 543.42 1.49 125 32 41 9 543.36 1.47 126 33 36 3 861.13 4.38 127 36 40 4 847.14 1.02 128 37 40 3 853.38 1.55 129 38 41 3 847.95 1.17 130 38 42 4 847.39 0.89 131 38 61 23 458.97 1.35 132 38 64 26 458.91 1.34 133 39 42 3 843.18 2.99 134 39 43 4 830.11 0.96 135 39 64 25 458.91 1.34 136 40 43 3 834.23 1.62 137 40 44 4 829.24 1.40 138 41 44 3 850.35 2.29 139 41 45 4 825.38 3.30 140 41 46 5 693.27 35.42 141 41 49 8 543.21 1.50 142 41 64 23 458.86 1.32 143 41 65 24 458.66 1.29 144 41 70 29 457.75 1.21 145 42 45 3 833.10 2.06 146 42 64 22 458.86 1.33 147 42 68 26 457.81 1.18 148 42 70 28 457.69 1.19 149 46 49 3 910.49 2.14 150 46 70 24 457.64 1.20 151 47 70 23 457.61 1.21 152 47 71 24 457.61 1.22 153 48 70 22 457.62 1.21 154 48 71 23 457.60 1.21 155 48 73 25 457.54 1.19 156 48 228 180 82.01 0.01 157 49 65 16 457.78 1.25 158 49 70 21 457.69 1.21 159 49 71 22 457.66 1.21 160 49 72 23 457.59 1.21 161 49 73 24 457.54 1.19 162 50 73 23 457.57 1.21 163 50 221 171 94.09 2.07 164 50 222 172 94.03 2.04 165 50 225 175 85.79 1.09 166 51 61 10 458.76 1.27 167 51 73 22 457.65 1.20 168 51 74 23 457.57 1.21 169 51 75 24 457.55 1.20 170 51 76 25 456.83 1.05 171 52 75 23 457.57 1.21 172 52 76 24 457.31 1.30 173 52 105 53 240.57 1.03 174 52 221 169 94.26 2.00 175 53 57 4 829.31 0.97 176 53 61 8 602.83 70.58 177 53 76 23 457.53 1.20 178 54 57 3 844.63 0.78 179 54 58 4 804.04 13.60 180 54 76 22 457.62 1.20 181 55 58 3 844.63 0.78 182 55 76 21 457.67 1.20 183 55 77 22 457.59 1.21 184 55 78 23 457.52 1.20 185 55 83 28 411.43 1.87 186 57 60 3 832.47 6.84 187 57 61 4 823.06 3.02 188 58 61 3 839.97 3.27 189 58 62 4 831.25 1.08 190 58 74 16 457.81 1.21 191 58 76 18 457.66 1.19 192 58 79 21 456.20 1.12 193 58 83 25 395.81 0.96 194 59 62 3 831.26 1.07 195 59 63 4 831.25 1.08 196 59 79 20 455.17 1.65 197 59 83 24 382.08 7.46 198 59 86 27 278.29 7.50 199 59 87 28 276.50 6.62 200 59 90 31 251.06 2.44 201 60 63 3 850.15 0.42 202 60 64 4 828.32 3.89 203 61 64 3 842.75 0.54 204 61 65 4 824.66 2.16 205 62 65 3 836.37 2.66 206 62 66 4 824.66 2.16 207 62 72 10 457.91 1.21 208 62 74 12 457.81 1.21 209 62 79 17 453.27 0.70 210 62 87 25 250.83 2.46 211 62 90 28 250.69 2.50 212 62 99 37 249.90 2.31 213 62 101 39 249.83 2.33 214 63 66 3 840.86 0.42 215 63 67 4 823.28 1.49 216 63 90 27 250.69 2.50 217 64 67 3 828.20 3.96 218 64 68 4 823.25 1.50 219 65 68 3 834.77 0.67 220 65 69 4 823.13 1.44 221 65 70 5 623.09 81.39 222 65 72 7 457.89 1.20 223 66 69 3 826.50 4.81 224 66 87 21 250.79 2.44 225 66 90 24 250.56 2.43 226 66 91 25 250.44 2.41 227 66 94 28 250.13 2.33 228 66 97 31 249.93 2.31 229 66 99 33 249.89 2.30 230 69 97 28 249.90 2.31 231 70 97 27 249.90 2.31 232 71 96 25 250.00 2.31 233 71 97 26 249.90 2.31 234 71 98 27 249.87 2.30 235 71 228 157 82.11 0.06 236 71 229 158 81.89 0.04 237 72 98 26 249.89 2.31 238 72 99 27 249.73 2.29 239 72 100 28 249.65 2.30 240 72 228 156 82.20 0.09 241 73 100 27 249.61 2.30 242 73 105 32 231.61 1.84 243 73 221 148 94.11 2.08 244 73 224 151 92.81 2.23 245 73 225 152 87.99 1.69 246 73 228 155 82.22 0.09 247 74 79 5 852.87 3.42 248 74 99 25 249.90 2.31 249 74 100 26 249.77 2.32 250 74 101 27 249.60 2.29 251 74 102 28 249.59 2.28 252 74 105 31 238.10 0.20 253 75 102 27 249.55 2.31 254 75 105 30 238.09 0.20 255 76 79 3 1032.79 3.33 256 78 83 5 830.50 7.37 257 79 83 4 817.22 0.73 258 79 84 5 817.23 0.73 259 79 87 8 784.75 5.40 260 79 101 22 249.84 2.31 261 80 83 3 833.35 5.94 262 80 84 4 817.25 0.73 263 81 84 3 843.42 0.91 264 81 85 4 817.25 0.73 265 82 85 3 843.41 0.91 266 82 86 4 820.17 0.72 267 83 86 3 823.15 0.93 268 83 87 4 820.17 0.72 269 84 87 3 833.15 6.05 270 84 88 4 827.80 8.72 271 84 101 17 250.06 2.32 272 84 234 150 76.64 0.95 273 84 236 152 71.55 0.74 274 85 88 3 837.77 3.74 275 85 89 4 824.33 2.99 276 86 89 3 837.82 3.72 277 86 90 4 818.71 5.84 278 87 90 3 837.84 3.71 279 87 91 4 826.00 2.21 280 87 99 12 250.10 2.31 281 87 101 14 250.07 2.32 282 87 234 147 74.25 0.17 283 88 91 3 843.50 0.89 284 88 92 4 837.85 3.71 285 88 234 146 74.25 0.17 286 89 92 3 842.00 1.71 287 90 93 3 867.22 2.35 288 90 94 4 833.52 1.55 289 91 94 3 847.55 3.84 290 91 95 4 819.75 0.94 291 91 97 6 754.90 4.04 292 91 99 8 250.12 2.31 293 91 233 142 74.25 0.18 294 91 234 143 74.08 0.26 295 92 95 3 858.50 0.09 296 94 97 3 859.66 3.31 297 96 232 136 71.44 0.84 298 97 233 136 64.66 0.20 299 98 228 130 82.23 0.07 300 98 229 131 82.13 0.07 301 98 231 133 79.61 1.18 302 98 232 134 66.45 0.09 303 98 233 135 64.66 0.20 304 99 233 134 69.11 0.08 305 99 234 135 62.07 1.08 306 99 235 136 62.00 1.11 307 100 105 5 854.90 1.25 308 100 224 124 92.91 2.27 309 100 228 128 84.88 0.64 310 100 235 135 57.06 0.58 311 100 237 137 53.80 0.27 312 101 234 133 65.02 0.03 313 101 235 134 61.27 1.41 314 101 236 135 53.84 0.29 315 101 237 136 53.80 0.28 316 102 105 3 859.99 1.29 317 102 237 135 53.47 0.43 318 103 113 10 745.34 2.35 319 103 143 40 117.53 0.42 320 103 146 43 130.16 1.93 321 103 237 134 53.81 0.28 322 104 109 5 776.45 2.43 323 104 110 6 765.95 7.67 324 104 113 9 752.31 0.85 325 104 143 39 128.00 2.87 326 104 217 113 95.57 1.68 327 105 217 112 95.53 1.68 328 105 220 115 94.36 1.96 329 105 221 116 94.38 1.96 330 105 224 119 94.38 1.96 331 105 238 133 51.84 0.65 332 106 109 3 825.86 2.43 333 106 110 4 821.10 1.83 334 106 209 103 117.95 0.29 335 106 211 105 114.98 1.09 336 106 216 110 95.71 1.69 337 106 217 111 95.58 1.70 338 107 110 3 835.07 5.15 339 107 111 4 821.08 1.82 340 107 220 113 95.59 1.69 341 107 238 131 52.70 0.60 342 107 242 135 41.48 2.35 343 108 111 3 831.55 3.41 344 108 112 4 821.05 1.83 345 108 209 101 117.95 0.29 346 109 112 3 830.78 1.56 347 109 113 4 833.01 2.68 348 109 209 100 117.97 0.29 349 110 113 3 840.97 1.30 350 110 114 4 828.88 0.61 351 110 238 128 52.91 0.70 352 110 239 129 52.72 0.60 353 110 242 132 48.20 0.77 354 111 114 3 832.51 0.70 355 111 115 4 815.50 4.66 356 112 115 3 838.93 6.67 357 112 116 4 815.53 4.66 358 112 207 95 134.52 0.82 359 112 209 97 133.11 0.41 360 113 116 3 847.93 2.17 361 113 117 4 825.00 0.08 362 113 143 30 139.19 2.23 363 113 146 33 139.41 2.31 364 113 147 34 139.15 2.21 365 113 150 37 143.34 0.42 366 114 117 3 840.32 0.91 367 114 118 4 827.85 2.85 368 115 118 3 834.18 0.32 369 115 119 4 826.04 0.60 370 115 207 92 139.15 2.21 371 116 119 3 838.88 1.79 372 116 120 4 811.52 0.54 373 116 147 31 143.10 0.54 374 116 151 35 166.00 7.50 375 116 196 80 139.17 2.21 376 116 198 82 173.16 3.91 377 117 120 3 845.04 0.21 378 117 150 33 152.99 0.83 379 117 154 37 386.80 2.64 380 118 122 4 866.16 1.87 381 119 122 3 863.91 3.00 382 119 123 4 847.82 2.59 383 119 198 79 265.36 3.99 384 119 205 86 193.35 2.33 385 120 123 3 874.40 4.21 386 120 151 31 623.36 3.52 387 120 154 34 651.73 0.27 388 120 155 35 653.62 0.68 389 120 198 78 304.79 0.42 390 121 154 33 657.78 2.75 391 121 155 34 656.53 0.77 392 121 156 35 721.58 0.99 393 121 158 37 722.49 0.86 394 123 155 32 722.71 0.79 395 123 198 75 335.05 0.77 396 123 199 76 354.81 0.41 397 123 200 77 355.42 0.66 398 123 205 82 354.00 0.41 399 124 155 31 722.73 0.79 400 124 161 37 722.85 0.82 401 124 162 38 722.88 0.81 402 124 199 75 355.48 0.65 403 124 200 76 355.44 0.66 404 125 161 36 722.85 0.82 405 125 162 37 722.93 0.80 406 125 198 73 355.48 0.65 407 125 199 74 355.49 0.66 408 126 151 25 722.69 0.79 409 126 161 35 722.94 0.80 410 126 180 54 722.46 0.87 411 126 181 55 722.36 0.91 412 126 197 71 355.60 0.67 413 126 198 72 355.51 0.65 414 127 161 34 722.99 0.81 415 127 173 46 722.67 0.81 416 127 180 53 722.68 0.80 417 127 181 54 722.36 0.91 418 127 183 56 721.84 1.12 419 127 196 69 356.12 0.60 420 127 197 70 355.93 0.52 421 128 144 16 722.62 0.82 422 128 147 19 722.68 0.81 423 128 151 23 722.84 0.80 424 128 180 52 722.72 0.80 425 128 181 53 722.67 0.80 426 128 182 54 722.25 0.92 427 128 183 55 721.85 1.11 428 128 194 66 356.80 0.68 429 128 195 67 356.64 0.64 430 128 196 68 356.12 0.59 431 129 183 54 722.32 0.93 432 129 185 56 721.83 1.12 433 129 194 65 356.82 0.68 434 129 195 66 356.64 0.64 435 129 197 68 356.60 0.66 436 130 140 10 722.67 0.80 437 130 141 11 722.68 0.81 438 130 144 14 722.70 0.80 439 130 183 53 722.66 0.80 440 130 184 54 722.28 0.90 441 130 185 55 721.84 1.12 442 130 186 56 721.84 1.12 443 130 193 63 357.32 0.47 444 130 194 64 356.93 0.64 445 131 185 54 722.09 0.99 446 131 190 59 358.37 0.42 447 131 192 61 357.50 0.47 448 131 193 62 357.33 0.46 449 131 194 63 357.31 0.47 450 132 136 4 816.98 2.48 451 132 141 9 722.75 0.81 452 132 185 53 722.67 0.80 453 132 186 54 722.25 0.92 454 132 187 55 721.84 1.12 455 132 190 58 367.94 5.19 456 132 191 59 358.32 0.42 457 132 192 60 357.60 0.42 458 132 193 61 357.49 0.47 459 133 136 3 863.35 2.93 460 133 186 53 722.66 0.81 461 133 187 54 722.29 0.90 462 133 190 57 470.51 45.12 463 133 191 58 358.35 0.42 464 133 214 81 355.30 0.59 465 134 186 52 722.68 0.81 466 134 187 53 722.66 0.81 467 134 188 54 722.37 0.92 468 136 141 5 780.87 9.48 469 136 186 50 722.73 0.81 470 138 186 48 722.95 0.78 471 139 184 45 723.00 0.79 472 139 186 47 722.98 0.79 473 140 144 4 812.04 4.77 474 140 175 35 723.15 0.80 475 140 177 37 723.22 0.80 476 140 182 42 723.15 0.80 477 140 184 44 723.00 0.79 478 140 186 46 723.00 0.79 479 141 144 3 832.08 5.25 480 141 145 4 816.68 2.45 481 141 186 45 723.07 0.80 482 142 145 3 839.45 1.04 483 142 146 4 816.68 2.45 484 143 146 3 844.45 1.46 485 143 147 4 816.69 2.45 486 143 194 51 357.34 0.46 487 144 147 3 854.65 1.62 488 144 148 4 819.12 1.20 489 144 182 38 723.21 0.79 490 144 194 50 357.25 0.48 491 145 148 3 833.59 2.03 492 145 149 4 827.03 5.16 493 145 177 32 723.26 0.81 494 146 149 3 827.20 5.23 495 146 150 4 819.10 1.19 496 146 236 90 76.67 2.64 497 147 150 3 825.06 3.68 498 147 151 4 829.27 3.60 499 147 194 47 357.29 0.46 500 147 196 49 356.56 0.59 501 148 151 3 837.19 0.35 502 148 152 4 829.27 3.60 503 148 177 29 723.39 0.79 504 148 180 32 723.39 0.79 505 148 182 34 723.29 0.81 506 149 152 3 837.19 0.35 507 149 153 4 828.88 4.50 508 149 177 28 723.53 0.76 509 149 178 29 723.54 0.76 510 150 153 3 835.45 0.52 511 150 154 4 825.13 2.63 512 151 154 3 835.24 0.41 513 151 155 4 833.22 1.41 514 151 180 29 723.61 0.79 515 151 196 45 356.47 0.60 516 151 198 47 356.13 0.60 517 152 155 3 846.20 5.08 518 152 156 4 833.22 1.41 519 152 178 26 723.63 0.78 520 152 180 28 723.62 0.79 521 153 156 3 846.96 1.36 522 155 158 3 847.52 1.64 523 155 159 4 847.52 1.64 524 155 161 6 790.60 5.79 525 155 180 25 723.72 0.81 526 161 181 20 723.09 0.51 527 163 173 10 723.80 0.83 528 165 168 3 901.01 3.48 529 165 173 8 723.86 0.81 530 169 172 3 824.95 2.60 531 171 174 3 802.15 5.16 532 172 183 11 724.38 0.84 533 173 181 8 724.44 0.83 534 173 183 10 724.40 0.82 535 174 182 8 724.41 0.84 536 174 183 9 724.38 0.83 537 175 180 5 771.41 20.82 538 175 181 6 724.76 0.96 539 175 182 7 724.54 0.84 540 176 179 3 838.22 4.03 541 176 180 4 771.41 20.82 542 177 180 3 819.87 3.40 543 177 182 5 735.06 2.69 544 185 190 5 818.68 9.02 545 187 190 3 845.49 3.56 546 190 193 3 937.93 5.12 547 191 211 20 355.73 0.71 548 191 212 21 355.58 0.69 549 191 213 22 355.38 0.61 550 191 214 23 355.27 0.58 551 192 210 18 355.74 0.71 552 192 211 19 355.40 0.60 553 192 217 25 287.88 8.45 554 193 209 16 355.74 0.71 555 193 210 17 355.50 0.64 556 193 211 18 355.40 0.60 557 194 208 14 355.73 0.71 558 194 209 15 355.50 0.65 559 195 206 11 355.88 0.70 560 195 207 12 355.73 0.71 561 195 208 13 355.50 0.65 562 196 205 9 355.94 0.73 563 196 206 10 355.73 0.71 564 196 207 11 355.71 0.71 565 197 205 8 355.94 0.73 566 197 206 9 355.86 0.76 567 198 204 6 356.01 0.72 568 198 205 7 355.95 0.73 569 198 207 9 355.96 0.73 570 199 203 4 735.29 0.98 571 199 204 5 356.00 0.72 572 200 203 3 862.49 8.88 573 211 216 5 733.22 2.00 574 211 217 6 733.23 1.99 575 213 216 3 844.87 1.02 576 214 217 3 851.50 2.30 577 215 218 3 850.68 1.85 578 215 219 4 850.68 1.85 579 216 219 3 893.33 0.34 580 217 221 4 816.83 3.50 581 218 221 3 835.94 1.99 582 218 222 4 813.91 4.96 583 219 222 3 843.14 1.93 584 219 223 4 813.89 4.96 585 220 223 3 840.87 1.86 586 220 224 4 821.31 1.26 587 220 238 18 49.98 0.09 588 221 224 3 840.87 1.86 589 221 225 4 812.56 0.97 590 222 225 3 823.88 6.63 591 222 226 4 812.59 0.96 592 223 226 3 837.26 3.31 593 223 227 4 815.17 2.25 594 223 238 15 47.97 0.88 595 223 241 18 34.65 0.51 596 223 242 19 33.56 0.48 597 224 227 3 835.75 4.08 598 224 228 4 812.86 1.11 599 224 238 14 47.98 0.87 600 224 241 17 33.61 0.52 601 225 228 3 833.32 5.29 602 227 230 3 846.55 3.77 603 227 241 14 33.61 0.52 604 227 244 17 32.88 0.29 605 227 245 18 25.30 0.82 606 228 231 3 871.80 3.92 607 231 235 4 821.77 2.75 608 231 240 9 42.98 1.68 609 231 241 10 38.40 0.54 610 235 240 5 814.71 0.04 611 237 240 3 909.68 0.60 612 238 241 3 855.40 1.18 613 238 242 4 843.48 2.16 614 239 242 3 845.11 1.34 615 239 243 4 819.07 10.04 616 241 245 4 845.90 1.20 617 242 245 3 852.86 2.52