BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 175 174 66.27 0.09 2 1 179 178 49.61 8.06 3 1 183 182 33.70 0.24 4 2 169 167 68.26 0.20 5 2 170 168 68.61 0.23 6 3 7 4 903.23 3.39 7 3 9 6 731.71 6.69 8 3 10 7 223.56 55.26 9 3 13 10 105.20 0.70 10 3 165 162 44.86 0.63 11 3 166 163 33.92 0.22 12 3 169 166 68.26 0.20 13 3 232 229 66.62 0.60 14 4 169 165 68.75 0.32 15 4 170 166 69.39 0.03 16 5 10 5 800.77 6.51 17 5 169 164 69.49 0.07 18 6 10 4 809.18 2.52 19 7 10 3 848.11 1.40 20 7 11 4 833.11 2.36 21 7 236 229 65.55 0.06 22 8 11 3 836.86 4.23 23 8 12 4 833.12 2.36 24 8 142 134 166.64 5.78 25 8 236 228 67.54 0.20 26 8 239 231 62.91 0.71 27 8 240 232 62.91 0.71 28 8 241 233 62.66 0.59 29 8 242 234 61.76 0.23 30 8 244 236 50.45 0.69 31 9 12 3 861.47 1.79 32 9 13 4 833.11 2.35 33 9 146 137 172.05 2.33 34 9 161 152 75.98 1.74 35 9 165 156 75.89 1.69 36 9 232 223 71.73 0.24 37 9 235 226 68.67 0.27 38 9 236 227 68.13 0.07 39 10 13 3 854.38 1.75 40 10 14 4 834.86 3.23 41 10 126 116 202.52 7.03 42 11 14 3 859.87 2.83 43 11 15 4 838.60 0.51 44 11 128 117 194.60 3.50 45 11 142 131 173.95 2.73 46 11 244 233 61.46 0.03 47 12 15 3 850.66 1.77 48 12 16 4 815.81 10.90 49 12 142 130 173.95 2.73 50 12 143 131 173.94 2.74 51 12 146 134 173.84 2.71 52 12 147 135 173.85 2.71 53 12 158 146 79.56 2.23 54 12 162 150 77.28 2.06 55 13 16 3 850.67 1.77 56 13 17 4 832.68 2.46 57 13 162 149 76.16 1.67 58 13 165 152 76.12 1.65 59 13 166 153 76.13 1.65 60 14 17 3 850.12 1.49 61 14 18 4 827.85 4.88 62 14 126 112 204.03 7.78 63 14 128 114 203.53 7.58 64 15 18 3 832.07 6.99 65 15 19 4 832.08 6.99 66 15 128 113 203.77 7.68 67 15 139 124 184.22 1.05 68 15 142 127 186.87 3.16 69 15 143 128 174.66 2.94 70 15 245 230 61.45 0.03 71 16 19 3 844.98 6.04 72 16 20 4 836.12 4.97 73 16 143 127 174.66 2.94 74 16 147 131 174.64 2.96 75 16 158 142 79.72 2.19 76 16 185 169 78.31 1.96 77 17 20 3 839.29 3.40 78 17 21 4 836.12 4.97 79 17 185 168 77.62 1.85 80 18 21 3 859.55 6.74 81 18 22 4 832.05 7.00 82 18 115 97 267.07 37.95 83 18 118 100 267.31 38.04 84 18 119 101 266.75 38.05 85 18 129 111 204.11 7.84 86 18 130 112 204.12 7.85 87 18 136 118 204.08 7.84 88 19 22 3 832.06 7.00 89 19 23 4 832.01 6.98 90 19 130 111 204.16 7.88 91 19 136 117 204.09 7.86 92 19 139 120 204.09 7.86 93 19 140 121 204.03 7.84 94 20 23 3 848.48 4.41 95 20 24 4 819.86 0.09 96 20 185 165 76.45 1.52 97 21 24 3 861.84 8.31 98 21 25 4 830.35 5.15 99 21 118 97 350.56 3.53 100 21 122 101 350.62 3.58 101 22 25 3 853.20 0.89 102 22 26 4 830.08 5.31 103 22 34 12 204.23 7.86 104 22 111 89 382.00 0.11 105 22 114 92 354.81 5.57 106 22 115 93 267.42 37.98 107 22 118 96 267.46 38.01 108 22 136 114 204.22 7.86 109 23 26 3 870.10 0.03 110 23 27 4 830.09 5.30 111 23 28 5 840.52 3.87 112 23 34 11 204.22 7.86 113 23 140 117 204.21 7.85 114 24 28 4 848.47 1.88 115 25 28 3 895.22 1.12 116 25 29 4 848.84 1.70 117 25 118 93 350.78 3.52 118 25 121 96 350.78 3.53 119 26 29 3 862.47 5.12 120 26 30 4 840.05 0.43 121 26 114 88 405.44 10.54 122 27 30 3 864.14 2.43 123 30 37 7 815.54 0.26 124 30 114 84 496.42 2.25 125 32 36 4 816.91 0.27 126 32 37 5 816.35 0.01 127 32 40 8 813.14 2.04 128 32 74 42 661.44 3.47 129 32 79 47 661.50 3.44 130 32 107 75 503.96 2.75 131 33 36 3 833.53 3.87 132 33 37 4 832.39 3.31 133 34 37 3 840.70 7.46 134 34 38 4 833.53 3.88 135 34 136 102 499.46 3.54 136 34 140 106 496.25 3.54 137 35 38 3 851.69 5.20 138 35 39 4 833.54 3.88 139 35 62 27 663.57 3.55 140 35 66 31 663.49 3.54 141 35 67 32 663.40 3.54 142 35 70 35 663.24 3.60 143 36 39 3 854.88 3.60 144 36 40 4 839.81 0.75 145 36 70 34 663.25 3.60 146 36 73 37 663.16 3.57 147 36 74 38 663.14 3.58 148 37 40 3 848.88 1.77 149 37 41 4 830.68 5.32 150 37 107 70 505.15 2.52 151 37 111 74 503.60 2.81 152 37 114 77 503.04 2.53 153 38 41 3 831.85 5.87 154 38 42 4 830.51 5.24 155 38 62 24 663.75 3.60 156 38 136 98 499.52 3.52 157 38 137 99 499.40 3.49 158 38 140 102 496.34 3.51 159 38 141 103 496.35 3.51 160 39 42 3 837.31 5.23 161 39 43 4 838.06 2.76 162 39 56 17 664.03 3.46 163 39 60 21 664.20 3.54 164 39 67 28 663.49 3.54 165 39 70 31 663.31 3.58 166 39 74 35 663.23 3.60 167 40 43 3 838.08 2.77 168 40 44 4 832.44 0.05 169 40 74 34 663.26 3.60 170 40 79 39 662.40 3.19 171 40 104 64 556.58 27.73 172 40 107 67 505.64 2.74 173 40 108 68 504.76 2.35 174 41 44 3 850.16 3.28 175 41 45 4 838.07 2.73 176 41 108 67 505.03 2.48 177 41 111 70 505.29 2.62 178 41 133 92 499.76 3.41 179 41 134 93 499.68 3.45 180 41 137 96 499.65 3.44 181 42 45 3 857.58 0.42 182 42 46 4 846.65 1.38 183 42 51 9 664.44 3.45 184 42 55 13 664.37 3.48 185 42 56 14 664.35 3.48 186 42 59 17 664.25 3.54 187 42 60 18 664.24 3.54 188 42 137 95 499.66 3.44 189 42 248 206 61.50 0.04 190 42 249 207 61.48 0.03 191 43 46 3 874.80 1.02 192 43 51 8 664.39 3.44 193 43 56 13 664.34 3.46 194 43 74 31 663.39 3.57 195 43 80 37 662.40 3.20 196 43 96 53 654.87 2.69 197 43 101 58 591.48 10.68 198 43 104 61 566.04 23.32 199 44 101 57 591.48 10.68 200 44 104 60 566.04 23.32 201 44 105 61 556.94 27.86 202 44 108 64 505.90 2.67 203 45 134 89 500.38 3.60 204 45 249 204 61.50 0.04 205 46 51 5 802.19 3.96 206 46 101 55 630.43 2.17 207 48 101 53 631.25 2.27 208 49 95 46 660.45 3.39 209 49 96 47 660.27 3.31 210 49 101 52 631.24 2.27 211 50 94 44 660.70 3.44 212 50 95 45 660.45 3.39 213 51 55 4 849.89 0.18 214 51 56 5 838.54 3.16 215 51 93 42 660.72 3.44 216 51 94 43 660.70 3.43 217 52 55 3 863.13 6.79 218 52 56 4 863.12 6.79 219 52 92 40 660.98 3.38 220 52 93 41 660.72 3.44 221 53 56 3 864.41 6.15 222 53 57 4 860.08 3.98 223 53 64 11 664.51 3.69 224 53 67 14 664.32 3.68 225 53 68 15 664.27 3.69 226 53 71 18 664.10 3.64 227 53 92 39 660.94 3.37 228 53 93 40 660.72 3.44 229 53 94 41 660.71 3.43 230 54 57 3 860.08 3.98 231 54 58 4 850.13 8.95 232 54 91 37 661.04 3.34 233 54 92 38 661.03 3.35 234 55 58 3 860.08 3.98 235 55 59 4 850.10 8.93 236 56 59 3 859.28 4.37 237 56 60 4 849.42 9.27 238 56 71 15 664.07 3.71 239 56 94 38 660.70 3.43 240 57 60 3 867.17 0.41 241 57 64 7 664.41 3.74 242 57 67 10 664.36 3.72 243 59 248 189 61.47 0.03 244 60 137 77 499.53 3.51 245 60 141 81 496.35 3.51 246 60 248 188 61.45 0.03 247 61 141 80 496.34 3.51 248 61 144 83 483.44 3.58 249 61 145 84 483.03 3.37 250 62 67 5 803.13 5.50 251 62 141 79 496.34 3.51 252 62 144 82 488.03 0.88 253 63 66 3 856.51 0.21 254 63 67 4 849.98 3.48 255 64 67 3 849.99 3.48 256 64 68 4 837.19 2.92 257 65 68 3 856.50 0.22 258 66 70 4 850.87 3.02 259 67 70 3 847.91 0.01 260 67 71 4 841.33 3.27 261 68 71 3 859.08 3.54 262 68 72 4 843.50 2.18 263 69 72 3 849.96 1.02 264 69 73 4 841.21 3.22 265 70 73 3 843.33 2.10 266 70 74 4 824.41 0.32 267 71 74 3 841.73 2.58 268 71 75 4 828.78 2.56 269 71 80 9 662.90 2.96 270 71 82 11 662.38 3.21 271 71 94 23 660.29 3.33 272 72 75 3 839.47 1.17 273 72 76 4 830.57 1.67 274 72 82 10 662.87 2.96 275 73 76 3 847.64 2.92 276 73 77 4 838.56 7.46 277 74 77 3 850.90 1.28 278 74 78 4 840.36 6.55 279 74 79 5 724.62 12.26 280 74 80 6 680.56 0.89 281 75 78 3 850.90 1.28 282 75 80 5 669.66 6.33 283 75 81 6 662.89 2.96 284 75 82 7 662.89 2.96 285 77 103 26 591.51 10.60 286 78 100 22 625.92 1.71 287 78 103 25 613.64 0.46 288 79 100 21 621.08 4.12 289 79 103 24 591.50 10.60 290 79 104 25 591.47 10.61 291 79 107 28 566.14 23.18 292 80 94 14 660.32 3.33 293 80 95 15 660.12 3.27 294 80 96 16 655.78 2.18 295 80 100 20 628.66 2.27 296 80 104 24 591.50 10.60 297 81 94 13 660.32 3.33 298 81 95 14 660.03 3.33 299 81 100 19 631.14 2.40 300 82 92 10 660.87 3.34 301 82 93 11 660.61 3.37 302 82 94 12 660.32 3.33 303 83 92 9 660.88 3.33 304 83 93 10 660.76 3.29 305 83 95 12 660.60 3.36 306 84 89 5 661.18 3.33 307 84 91 7 661.10 3.31 308 85 88 3 871.63 1.42 309 85 89 4 661.23 3.37 310 85 91 6 661.12 3.33 311 85 93 8 661.00 3.35 312 88 91 3 853.99 1.21 313 95 100 5 840.31 1.95 314 96 100 4 840.31 1.95 315 96 101 5 830.96 2.71 316 96 104 8 806.28 3.54 317 97 100 3 842.09 2.42 318 97 101 4 840.35 1.93 319 98 101 3 842.25 0.98 320 98 102 4 844.89 0.66 321 99 102 3 847.23 0.51 322 99 103 4 844.90 0.66 323 100 103 3 855.86 3.81 324 100 104 4 837.82 0.64 325 101 104 3 845.10 2.31 326 101 105 4 832.49 3.30 327 102 105 3 840.10 0.19 328 102 106 4 842.24 1.57 329 103 106 3 854.39 3.60 330 103 107 4 842.72 1.34 331 104 107 3 842.75 1.33 332 104 108 4 842.73 1.34 333 105 108 3 847.03 0.54 334 105 109 4 842.73 1.34 335 106 109 3 845.70 0.14 336 106 110 4 841.00 2.21 337 107 110 3 858.21 9.08 338 107 111 4 836.75 1.66 339 108 111 3 843.47 1.70 340 108 112 4 836.75 1.66 341 108 133 25 500.38 3.57 342 109 112 3 845.38 4.24 343 109 113 4 836.76 0.09 344 110 113 3 847.08 0.53 345 110 114 4 836.37 0.21 346 111 114 3 849.98 6.64 347 111 115 4 840.62 2.34 348 111 133 22 500.47 3.56 349 111 136 25 500.48 3.57 350 112 115 3 856.24 3.51 351 112 116 4 840.61 2.34 352 112 132 20 500.48 3.56 353 112 133 21 500.48 3.56 354 113 116 3 854.25 4.50 355 113 117 4 840.62 2.35 356 114 117 3 854.25 4.50 357 114 118 4 833.83 1.04 358 115 118 3 854.24 4.50 359 115 119 4 849.58 6.83 360 115 130 15 500.66 3.52 361 115 131 16 500.66 3.52 362 115 132 17 500.65 3.51 363 115 133 18 500.65 3.51 364 115 136 21 500.63 3.52 365 116 119 3 837.10 0.58 366 116 120 4 833.84 1.04 367 117 120 3 845.12 5.75 368 117 121 4 832.69 0.47 369 118 121 3 840.24 1.30 370 118 122 4 831.21 0.23 371 119 122 3 831.80 2.92 372 119 124 5 625.12 49.30 373 119 129 10 500.74 3.52 374 119 130 11 500.74 3.52 375 119 131 12 500.74 3.52 376 124 129 5 708.86 7.41 377 126 129 3 892.38 0.69 378 127 131 4 894.87 1.93 379 128 139 11 501.11 3.42 380 128 245 117 61.30 0.10 381 129 139 10 501.12 3.41 382 130 135 5 506.84 4.00 383 130 136 6 503.40 2.28 384 130 139 9 501.16 3.40 385 131 135 4 816.14 3.31 386 132 135 3 844.12 3.66 387 132 136 4 824.80 0.61 388 133 136 3 838.96 1.11 389 133 137 4 824.94 0.55 390 134 137 3 846.55 3.58 391 134 138 4 832.72 3.33 392 134 249 115 61.06 0.12 393 134 250 116 61.08 0.12 394 135 138 3 855.56 4.17 395 135 139 4 832.73 3.33 396 136 139 3 850.74 0.96 397 136 140 4 833.22 2.16 398 137 140 3 850.50 1.09 399 137 141 4 838.71 0.56 400 137 248 111 61.28 0.10 401 137 249 112 61.12 0.10 402 138 141 3 839.45 0.19 403 138 142 4 838.72 0.55 404 138 245 107 61.49 0.03 405 138 246 108 61.47 0.03 406 138 247 109 61.41 0.05 407 138 248 110 61.13 0.10 408 138 249 111 60.76 0.20 409 139 142 3 844.96 3.14 410 139 143 4 838.72 0.55 411 139 245 106 61.45 0.03 412 140 143 3 848.55 1.34 413 140 144 4 838.72 0.55 414 141 144 3 847.07 2.08 415 141 145 4 833.38 2.12 416 141 247 106 61.30 0.10 417 141 248 107 61.13 0.10 418 142 145 3 838.59 0.60 419 142 146 4 826.82 1.17 420 142 239 97 68.31 0.18 421 142 244 102 61.48 0.03 422 142 245 103 61.47 0.03 423 142 247 105 61.22 0.06 424 143 146 3 851.76 10.09 425 143 147 4 833.28 2.06 426 144 147 3 841.12 1.91 427 144 148 4 833.47 2.17 428 145 148 3 839.52 0.35 429 145 149 4 829.68 4.07 430 145 239 94 73.39 0.99 431 145 242 97 67.53 0.03 432 145 247 102 61.18 0.03 433 146 149 3 839.51 0.35 434 146 150 4 849.01 5.59 435 146 161 15 79.71 2.19 436 146 235 89 76.03 1.33 437 146 239 93 70.19 0.09 438 147 150 3 849.10 5.56 439 147 151 4 849.01 5.60 440 147 158 11 79.74 2.20 441 148 151 3 852.90 3.66 442 148 152 4 848.83 5.69 443 149 152 3 848.84 5.69 444 149 153 4 834.49 1.49 445 149 238 89 76.23 1.64 446 149 239 90 75.14 1.22 447 150 153 3 851.07 0.42 448 150 154 4 815.75 4.70 449 150 156 6 348.73 8.98 450 150 157 7 214.27 48.39 451 150 158 8 214.23 48.40 452 150 161 11 95.14 6.90 453 150 235 85 76.59 1.53 454 153 238 85 77.09 1.71 455 154 161 7 737.99 18.80 456 154 231 77 82.70 0.68 457 154 235 81 78.75 2.50 458 156 160 4 812.80 5.70 459 156 164 8 782.57 2.38 460 156 198 42 263.39 4.67 461 156 201 45 281.70 4.49 462 156 203 47 263.39 4.67 463 157 160 3 834.20 3.66 464 157 161 4 817.99 3.11 465 158 161 3 833.53 1.32 466 158 162 4 826.07 4.83 467 158 186 28 307.31 0.17 468 159 162 3 836.98 0.64 469 159 163 4 826.07 4.82 470 159 186 27 307.14 0.24 471 159 194 35 305.49 0.98 472 160 163 3 839.23 1.74 473 160 164 4 835.57 0.10 474 160 194 34 305.49 0.97 475 160 197 37 305.49 0.98 476 160 198 38 305.48 0.98 477 161 164 3 837.11 0.57 478 161 165 4 835.58 0.10 479 161 231 70 109.39 2.78 480 161 232 71 105.95 2.28 481 161 235 74 103.91 1.28 482 162 165 3 844.90 2.18 483 162 166 4 835.68 0.15 484 162 186 24 307.42 0.11 485 163 166 3 842.33 0.89 486 163 167 4 835.64 0.13 487 163 184 21 307.64 0.24 488 163 191 28 307.18 0.21 489 163 194 31 307.14 0.20 490 163 195 32 307.14 0.19 491 163 198 35 307.13 0.20 492 164 167 3 856.13 7.88 493 164 168 4 835.65 0.14 494 164 198 34 307.13 0.20 495 164 203 39 306.79 0.07 496 164 228 64 123.10 3.64 497 164 231 67 122.71 3.85 498 165 168 3 841.85 2.96 499 165 169 4 838.80 0.79 500 165 232 67 112.70 1.12 501 166 169 3 874.94 1.52 502 166 170 4 848.03 1.65 503 166 180 14 307.77 0.29 504 166 184 18 307.79 0.29 505 167 170 3 853.19 0.66 506 167 175 8 307.78 0.30 507 167 180 13 307.78 0.29 508 167 204 37 306.85 0.05 509 167 218 51 305.96 0.36 510 167 220 53 302.27 2.19 511 168 171 3 865.06 3.45 512 168 220 52 303.00 1.81 513 168 225 57 229.96 1.67 514 168 228 60 126.57 5.17 515 168 229 61 123.35 3.58 516 168 232 64 123.30 3.56 517 170 173 3 844.60 11.34 518 170 175 5 626.61 40.64 519 171 225 54 279.35 0.14 520 172 220 48 305.92 0.36 521 172 222 50 303.10 1.77 522 172 225 53 279.39 0.12 523 173 219 46 306.25 0.32 524 174 217 43 306.47 0.26 525 174 218 44 306.42 0.27 526 174 219 45 306.25 0.32 527 175 180 5 791.12 4.96 528 175 217 42 306.46 0.26 529 175 218 43 306.32 0.32 530 176 179 3 842.34 0.62 531 176 180 4 829.12 1.06 532 176 215 39 306.56 0.27 533 176 216 40 306.53 0.28 534 176 217 41 306.45 0.29 535 177 180 3 835.29 4.15 536 177 181 4 829.12 1.06 537 177 191 14 308.47 0.55 538 177 192 15 308.45 0.55 539 177 195 18 308.33 0.52 540 177 216 39 306.50 0.29 541 177 218 41 306.32 0.33 542 178 181 3 850.99 4.86 543 178 182 4 829.11 1.06 544 179 182 3 834.08 1.43 545 179 183 4 821.40 4.90 546 180 183 3 845.45 2.94 547 180 184 4 833.41 1.13 548 180 191 11 308.49 0.56 549 180 195 15 308.46 0.55 550 180 218 38 306.32 0.32 551 181 184 3 858.22 2.06 552 181 188 7 308.66 0.59 553 181 191 10 308.47 0.55 554 184 191 7 761.97 3.70 555 186 191 5 781.19 1.69 556 187 190 3 867.25 6.58 557 187 191 4 828.49 0.12 558 188 191 3 840.94 3.84 559 188 192 4 827.34 2.95 560 189 192 3 858.73 1.74 561 189 193 4 825.71 2.14 562 190 193 3 852.50 4.85 563 190 194 4 827.50 2.89 564 191 194 3 842.31 3.88 565 191 195 4 836.30 6.88 566 192 195 3 840.21 5.70 567 192 196 4 826.24 1.93 568 193 196 3 834.89 0.85 569 193 197 4 827.56 1.32 570 194 197 3 864.73 6.07 571 194 198 4 828.89 2.00 572 195 198 3 851.58 3.56 573 195 199 4 827.68 1.39 574 195 204 9 317.05 0.99 575 195 218 23 305.86 0.85 576 196 199 3 838.67 2.89 577 196 200 4 837.45 3.50 578 196 206 10 309.94 0.26 579 197 200 3 845.23 0.39 580 197 201 4 837.45 3.50 581 198 201 3 853.21 1.15 582 198 202 4 826.46 2.00 583 198 203 5 594.99 45.52 584 198 204 6 594.99 45.52 585 199 202 3 842.98 6.25 586 199 204 5 336.15 9.46 587 199 206 7 310.01 0.29 588 202 224 22 249.80 10.39 589 203 220 17 304.07 1.95 590 203 224 21 249.76 10.38 591 203 227 24 215.13 6.62 592 204 218 14 306.26 0.99 593 204 219 15 306.05 0.97 594 204 220 16 304.07 1.95 595 204 224 20 274.82 2.14 596 205 218 13 307.05 0.60 597 205 219 14 306.17 0.99 598 205 221 16 304.97 1.50 599 205 224 19 293.69 7.06 600 206 216 10 307.50 0.62 601 206 217 11 307.27 0.68 602 206 218 12 306.31 1.01 603 207 216 9 307.51 0.62 604 207 217 10 307.42 0.66 605 207 219 12 307.31 0.70 606 208 213 5 312.28 0.01 607 208 215 7 307.62 0.64 608 208 216 8 307.53 0.62 609 208 217 9 307.50 0.62 610 209 212 3 831.13 0.55 611 209 213 4 316.00 1.88 612 209 215 6 307.63 0.64 613 209 217 8 307.60 0.64 614 212 215 3 869.62 4.51 615 220 224 4 788.58 8.29 616 220 225 5 755.64 0.61 617 220 228 8 654.70 48.28 618 221 224 3 839.77 2.64 619 221 225 4 827.80 0.69 620 222 225 3 836.25 0.33 621 222 226 4 831.28 1.05 622 223 226 3 851.48 0.61 623 223 227 4 840.72 1.90 624 224 227 3 849.61 1.55 625 224 228 4 831.13 1.13 626 225 228 3 854.19 1.79 627 225 229 4 831.12 1.12 628 226 229 3 852.15 2.82 629 226 230 4 849.32 1.41 630 227 230 3 854.20 1.79 631 227 231 4 849.32 1.42 632 228 231 3 849.90 1.71 633 228 232 4 842.70 4.73 634 229 232 3 848.99 2.17 635 229 233 4 843.12 4.53 636 230 233 3 844.15 5.04 637 230 234 4 843.12 4.53 638 231 234 3 856.50 2.17 639 231 235 4 843.11 4.53 640 232 235 3 856.68 2.08 641 232 236 4 832.41 0.83 642 233 236 3 838.09 1.78 643 233 237 4 832.41 0.82 644 234 237 3 847.43 2.97 645 234 238 4 832.34 0.78 646 235 238 3 843.61 4.88 647 235 239 4 831.98 0.60 648 236 239 3 843.60 4.89 649 236 240 4 831.38 0.89 650 237 240 3 840.76 5.95 651 239 242 3 873.73 6.19 652 242 247 5 830.80 0.76 653 243 246 3 847.65 2.21 654 243 247 4 830.85 0.74 655 244 247 3 863.07 2.43 656 246 249 3 854.07 0.56 657 246 250 4 580.39 99.68