BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 79 77 50.80 0.14 2 2 80 78 39.24 1.20 3 3 75 72 53.58 0.04 4 4 42 38 122.35 5.08 5 4 43 39 109.02 0.39 6 4 71 67 53.61 0.04 7 4 72 68 53.59 0.04 8 4 75 71 53.62 0.03 9 5 12 7 779.45 6.42 10 5 40 35 128.70 2.02 11 5 60 55 108.28 0.01 12 5 72 67 53.60 0.04 13 5 75 70 53.62 0.03 14 5 80 75 155.24 1.67 15 6 39 33 128.71 2.02 16 6 40 34 128.59 1.98 17 6 42 36 123.39 4.55 18 7 12 5 784.81 3.73 19 7 39 32 155.35 1.61 20 7 80 73 171.85 0.35 21 8 80 72 171.85 0.35 22 9 79 70 193.26 3.44 23 9 80 71 183.05 1.48 24 9 81 72 171.85 0.35 25 10 56 46 305.45 0.93 26 11 37 26 292.25 0.97 27 11 39 28 269.50 0.95 28 11 49 38 269.38 1.00 29 12 39 27 269.37 1.01 30 12 40 28 259.61 2.48 31 12 49 37 267.97 1.69 32 12 56 44 305.80 0.80 33 12 58 46 298.56 1.03 34 12 60 48 265.25 1.15 35 12 76 64 209.91 0.50 36 12 80 68 209.93 0.49 37 12 82 70 209.93 0.49 38 13 16 3 926.97 1.51 39 13 49 36 269.37 1.00 40 13 56 43 305.80 0.80 41 13 57 44 305.72 0.79 42 13 58 45 291.55 0.61 43 13 82 69 209.93 0.49 44 14 55 41 305.91 0.79 45 14 82 68 210.06 0.56 46 14 83 69 209.92 0.50 47 14 84 70 209.92 0.50 48 15 55 40 305.98 0.79 49 15 57 42 305.76 0.79 50 15 58 43 305.75 0.79 51 15 82 67 210.06 0.55 52 15 84 69 209.90 0.51 53 15 232 217 67.81 0.73 54 16 58 42 305.77 0.79 55 16 59 43 305.74 0.79 56 16 76 60 211.12 0.60 57 16 82 66 210.63 0.47 58 16 84 68 209.92 0.50 59 16 85 69 209.80 0.51 60 16 86 70 209.78 0.49 61 17 28 11 320.06 2.04 62 17 57 40 305.93 0.78 63 17 58 41 305.79 0.79 64 17 59 42 305.75 0.79 65 17 60 43 305.74 0.79 66 17 86 69 209.81 0.51 67 17 225 208 95.14 0.72 68 17 228 211 85.62 1.21 69 17 229 212 80.47 1.85 70 18 60 42 305.75 0.78 71 18 61 43 305.75 0.79 72 18 62 44 305.74 0.79 73 18 72 54 227.41 3.17 74 18 73 55 216.66 1.42 75 18 76 58 216.69 1.40 76 18 85 67 210.53 0.48 77 18 86 68 209.92 0.50 78 18 87 69 209.81 0.52 79 18 88 70 209.75 0.49 80 19 24 5 834.24 2.16 81 19 25 6 785.37 2.06 82 19 59 40 305.91 0.78 83 19 60 41 305.79 0.79 84 19 61 42 305.72 0.81 85 19 62 43 305.67 0.82 86 19 88 69 209.75 0.49 87 20 24 4 842.65 2.04 88 20 62 42 305.69 0.82 89 20 63 43 305.69 0.82 90 20 87 67 210.58 0.48 91 22 25 3 812.83 2.09 92 22 26 4 800.89 4.34 93 22 44 22 305.92 0.79 94 22 45 23 305.78 0.77 95 22 61 39 305.94 0.79 96 23 26 3 805.77 5.62 97 23 27 4 815.24 0.88 98 24 27 3 828.53 4.76 99 24 28 4 796.39 2.71 100 24 88 64 215.56 1.03 101 24 89 65 210.89 0.46 102 24 221 197 96.00 0.65 103 25 28 3 794.14 3.83 104 25 29 4 793.23 4.29 105 25 46 21 306.06 0.85 106 25 48 23 306.06 0.85 107 25 59 34 306.29 0.79 108 25 60 35 306.00 0.81 109 25 61 36 305.94 0.79 110 26 29 3 793.96 0.72 111 26 30 4 786.48 1.19 112 26 48 22 306.06 0.85 113 27 30 3 795.80 0.33 114 27 31 4 777.23 3.43 115 27 32 5 774.26 4.90 116 27 218 191 96.07 0.67 117 27 221 194 95.98 0.64 118 27 222 195 95.92 0.63 119 28 31 3 797.56 0.27 120 28 32 4 774.10 4.92 121 28 33 5 773.97 4.86 122 28 59 31 306.33 0.78 123 28 221 193 96.00 0.64 124 28 225 197 95.66 0.71 125 29 33 4 772.41 4.11 126 29 36 7 772.37 4.08 127 29 48 19 306.11 0.84 128 29 59 30 306.30 0.79 129 32 52 20 318.51 2.12 130 32 222 190 95.91 0.65 131 32 225 193 95.66 0.71 132 32 226 194 94.61 0.69 133 32 229 197 94.07 0.81 134 33 50 17 318.56 2.13 135 33 51 18 318.52 2.12 136 33 52 19 318.51 2.12 137 33 57 24 318.41 2.18 138 33 59 26 317.98 2.04 139 33 229 196 83.61 0.47 140 34 50 16 318.53 2.12 141 34 51 17 318.51 2.12 142 36 49 13 319.13 2.25 143 36 50 14 319.07 2.23 144 37 48 11 319.31 2.22 145 37 49 12 319.13 2.25 146 37 56 19 318.88 2.30 147 38 47 9 319.66 2.12 148 38 48 10 319.33 2.20 149 39 47 8 319.58 2.08 150 39 48 9 319.32 2.19 151 39 49 10 319.17 2.26 152 40 45 5 846.68 0.65 153 40 46 6 759.73 8.54 154 40 47 7 319.67 2.12 155 40 60 20 318.65 2.21 156 41 45 4 827.73 2.60 157 41 46 5 452.67 42.84 158 42 45 3 798.79 3.11 159 43 72 29 223.50 1.82 160 45 60 15 319.14 2.25 161 45 61 16 318.82 2.31 162 45 62 17 318.63 2.29 163 45 63 18 318.63 2.29 164 45 72 27 283.80 12.30 165 46 60 14 319.09 2.25 166 47 59 12 319.09 2.25 167 47 60 13 318.66 2.27 168 48 58 10 319.36 2.40 169 48 59 11 318.61 2.29 170 49 56 7 779.68 0.00 171 49 57 8 320.75 1.84 172 49 58 9 318.75 2.31 173 50 56 6 749.56 1.54 174 50 57 7 320.64 1.90 175 51 55 4 827.62 1.55 176 51 56 5 763.08 8.29 177 52 55 3 805.31 2.54 178 52 57 5 320.64 1.90 179 52 229 177 83.09 0.70 180 52 230 178 79.09 1.39 181 52 233 181 65.50 0.43 182 53 230 177 78.34 1.15 183 53 233 180 67.78 0.75 184 55 232 177 79.92 1.81 185 55 233 178 74.95 0.04 186 55 236 181 64.50 0.59 187 57 229 172 81.16 1.61 188 57 232 175 76.76 0.85 189 60 72 12 269.30 27.39 190 60 76 16 216.72 1.41 191 62 68 6 827.66 1.43 192 62 69 7 774.62 1.36 193 62 72 10 300.70 30.57 194 62 87 25 210.59 0.48 195 62 181 119 217.03 1.61 196 62 185 123 217.18 1.54 197 63 68 5 811.75 1.91 198 64 68 4 840.02 1.50 199 64 181 117 217.03 1.61 200 65 68 3 1007.87 0.22 201 65 69 4 798.41 0.46 202 65 180 115 217.03 1.61 203 65 181 116 217.03 1.61 204 66 69 3 824.48 4.46 205 66 70 4 813.32 1.32 206 66 111 45 217.23 1.55 207 66 113 47 217.38 1.49 208 66 115 49 217.64 1.48 209 66 155 89 217.06 1.61 210 66 157 91 217.06 1.62 211 66 180 114 217.05 1.62 212 66 181 115 217.03 1.61 213 66 183 117 217.09 1.60 214 66 184 118 217.22 1.55 215 67 70 3 816.72 0.38 216 67 71 4 806.74 4.62 217 68 71 3 813.33 1.33 218 68 72 4 813.38 1.35 219 69 72 3 824.27 1.88 220 69 73 4 806.92 4.58 221 69 181 112 217.05 1.62 222 69 185 116 217.26 1.52 223 70 73 3 809.77 5.37 224 70 74 4 806.76 4.65 225 70 188 118 217.78 1.54 226 71 74 3 807.56 4.27 227 71 75 4 806.75 4.66 228 72 75 3 811.70 2.19 229 72 76 4 793.21 3.13 230 73 76 3 806.76 4.66 231 73 77 4 781.32 2.81 232 73 85 12 326.42 4.88 233 73 185 112 217.88 1.54 234 73 188 115 218.53 1.35 235 74 77 3 808.60 0.39 236 74 78 4 781.32 2.81 237 74 188 114 218.03 1.55 238 74 192 118 337.15 3.91 239 75 78 3 797.38 5.22 240 75 79 4 772.71 0.55 241 75 80 5 772.70 0.56 242 76 80 4 763.52 1.66 243 76 81 5 763.49 1.65 244 76 82 6 763.48 1.65 245 76 85 9 552.88 94.98 246 77 81 4 742.39 11.95 247 77 82 5 742.39 11.95 248 77 85 8 348.09 5.95 249 77 188 111 353.15 3.92 250 77 192 115 356.76 2.34 251 77 194 117 356.80 2.33 252 78 192 114 353.16 3.92 253 82 194 112 498.28 0.05 254 83 194 111 498.31 0.06 255 83 195 112 498.22 0.04 256 84 133 49 498.27 0.06 257 84 195 111 498.21 0.04 258 84 235 151 60.45 1.06 259 85 133 48 498.27 0.06 260 85 185 100 498.49 0.07 261 85 195 110 498.30 0.06 262 85 196 111 498.22 0.04 263 85 197 112 498.17 0.07 264 86 133 47 498.31 0.06 265 86 197 111 498.22 0.04 266 86 199 113 498.22 0.04 267 86 228 142 87.66 1.55 268 86 231 145 80.02 2.01 269 86 232 146 71.29 1.01 270 87 179 92 498.22 0.04 271 87 181 94 498.69 0.10 272 87 185 98 498.54 0.07 273 87 197 110 498.29 0.05 274 87 198 111 498.22 0.05 275 87 199 112 498.19 0.06 276 88 199 111 498.22 0.04 277 88 224 136 95.98 0.64 278 88 225 137 95.27 0.80 279 88 228 140 88.09 1.33 280 89 199 110 498.29 0.05 281 89 200 111 498.22 0.05 282 89 201 112 498.22 0.04 283 89 224 135 95.97 0.65 284 90 179 89 499.45 0.22 285 90 200 110 498.29 0.05 286 90 201 111 498.22 0.04 287 90 203 113 498.22 0.04 288 90 217 127 96.32 0.64 289 91 177 86 499.62 0.24 290 91 178 87 499.53 0.21 291 91 179 88 499.44 0.22 292 91 200 109 498.34 0.06 293 91 201 110 498.23 0.04 294 91 202 111 498.22 0.04 295 91 203 112 498.22 0.04 296 92 177 85 499.62 0.24 297 92 178 86 499.35 0.27 298 92 204 112 498.21 0.04 299 92 206 114 494.13 1.15 300 93 98 5 879.86 1.27 301 93 176 83 499.69 0.26 302 93 177 84 499.65 0.24 303 93 202 109 498.35 0.06 304 93 204 111 498.22 0.04 305 93 205 112 497.92 0.12 306 93 206 113 460.38 1.79 307 94 167 73 500.66 0.35 308 94 170 76 500.68 0.36 309 94 171 77 500.61 0.35 310 94 175 81 499.90 0.32 311 94 178 84 499.59 0.23 312 94 205 111 498.23 0.05 313 94 206 112 463.80 4.07 314 94 207 113 452.45 1.60 315 95 171 76 500.60 0.34 316 95 174 79 500.58 0.35 317 95 175 80 499.90 0.32 318 95 176 81 499.75 0.27 319 95 205 110 498.29 0.04 320 96 171 75 500.68 0.36 321 97 149 52 527.37 11.15 322 98 149 51 572.60 25.59 323 98 176 78 500.67 0.35 324 98 202 104 498.76 0.01 325 98 205 107 498.45 0.06 326 99 202 103 498.75 0.01 327 99 210 111 464.47 8.10 328 100 202 102 498.76 0.01 329 100 203 103 498.48 0.06 330 100 205 105 498.45 0.06 331 100 210 110 433.54 0.95 332 100 212 112 369.59 0.15 333 101 202 101 498.76 0.01 334 101 212 111 99.76 0.39 335 101 216 115 98.47 0.25 336 101 217 116 97.48 0.19 337 101 219 118 97.05 0.44 338 101 220 119 96.98 0.42 339 102 149 47 715.07 0.30 340 102 201 99 498.85 0.03 341 102 202 100 498.60 0.01 342 103 146 43 715.33 0.34 343 103 147 44 715.26 0.33 344 103 148 45 715.18 0.35 345 103 149 46 715.00 0.27 346 103 199 96 499.09 0.05 347 103 200 97 498.87 0.03 348 103 201 98 498.80 0.02 349 104 149 45 715.00 0.27 350 104 153 49 714.84 0.20 351 104 176 72 595.26 0.58 352 104 199 95 498.89 0.03 353 104 200 96 498.79 0.02 354 104 202 98 498.59 0.01 355 105 134 29 715.66 0.33 356 105 137 32 715.57 0.31 357 105 138 33 715.52 0.33 358 105 148 43 715.31 0.34 359 105 149 44 715.19 0.33 360 105 150 45 714.85 0.20 361 105 151 46 714.85 0.20 362 105 197 92 499.42 0.11 363 105 198 93 498.88 0.03 364 105 199 94 498.74 0.01 365 106 129 23 715.79 0.34 366 106 151 45 715.07 0.30 367 106 153 47 714.98 0.26 368 106 186 80 509.68 3.16 369 106 198 92 498.74 0.01 370 107 128 21 715.88 0.34 371 107 129 22 715.77 0.35 372 107 134 27 715.67 0.33 373 107 150 43 715.30 0.34 374 107 151 44 715.25 0.33 375 108 127 19 715.93 0.33 376 108 128 20 715.91 0.33 377 108 151 43 715.28 0.34 378 108 152 44 715.25 0.33 379 108 153 45 715.24 0.33 380 108 186 78 512.33 4.45 381 109 127 18 715.92 0.33 382 109 152 43 715.28 0.34 383 109 153 44 715.24 0.33 384 109 155 46 715.24 0.34 385 109 182 73 577.38 4.99 386 109 183 74 528.09 2.69 387 109 186 77 531.33 4.30 388 110 152 42 715.34 0.35 389 110 153 43 715.25 0.34 390 110 154 44 715.24 0.33 391 110 155 45 715.22 0.34 392 110 175 65 595.45 0.57 393 110 183 73 528.09 2.69 394 111 154 43 715.25 0.34 395 111 155 44 715.24 0.34 396 111 183 72 570.36 2.04 397 112 154 42 715.34 0.34 398 112 155 43 715.25 0.34 399 112 156 44 715.22 0.34 400 112 157 45 715.22 0.34 401 113 157 44 715.24 0.33 402 114 117 3 900.85 0.89 403 114 118 4 819.93 0.19 404 115 118 3 815.39 0.10 405 115 119 4 788.59 0.27 406 116 119 3 810.78 2.20 407 116 120 4 799.65 3.14 408 116 125 9 717.00 0.13 409 116 127 11 716.27 0.35 410 116 183 67 594.23 0.52 411 116 187 71 552.33 0.36 412 117 120 3 788.88 0.12 413 117 121 4 787.28 0.39 414 117 122 5 788.88 0.12 415 117 123 6 778.93 3.30 416 119 187 68 592.31 1.40 417 119 188 69 548.60 2.16 418 119 191 72 548.05 2.06 419 120 123 3 880.06 3.79 420 120 124 4 830.08 2.23 421 120 125 5 802.19 0.53 422 125 187 62 556.24 0.66 423 125 190 65 547.68 2.21 424 127 183 56 587.36 0.79 425 127 186 59 548.74 2.14 426 128 186 58 531.43 3.95 427 129 134 5 825.76 2.17 428 129 186 57 512.75 3.81 429 129 196 67 499.90 0.36 430 129 197 68 498.69 0.05 431 130 134 4 805.05 3.85 432 130 195 65 500.40 0.16 433 130 196 66 499.84 0.34 434 131 134 3 805.05 3.85 435 131 135 4 800.51 6.12 436 132 135 3 800.52 6.12 437 132 136 4 800.51 6.12 438 133 136 3 812.00 0.38 439 133 137 4 786.64 0.79 440 133 195 62 500.50 0.11 441 133 196 63 500.08 0.24 442 133 197 64 500.01 0.22 443 134 137 3 796.02 1.27 444 134 138 4 762.39 5.97 445 134 150 16 715.45 0.32 446 134 197 63 499.65 0.18 447 135 138 3 799.89 1.09 448 135 139 4 777.26 11.14 449 136 139 3 784.40 8.84 450 136 140 4 777.28 11.14 451 136 231 95 81.23 1.63 452 136 234 98 79.48 2.21 453 136 235 99 64.23 1.03 454 137 140 3 794.20 4.16 455 137 141 4 783.14 8.21 456 137 197 60 499.95 0.24 457 137 199 62 499.44 0.12 458 137 227 90 94.80 0.51 459 137 231 94 80.97 1.76 460 138 141 3 796.81 1.38 461 138 142 4 773.40 1.40 462 138 150 12 715.36 0.34 463 139 142 3 799.00 2.48 464 139 143 4 785.13 4.46 465 139 234 95 80.80 1.84 466 140 143 3 786.05 4.00 467 140 144 4 785.13 4.45 468 140 227 87 95.48 0.60 469 140 230 90 94.38 0.49 470 140 231 91 90.42 2.06 471 140 234 94 80.78 1.86 472 141 144 3 790.42 1.81 473 141 145 4 785.13 4.45 474 141 146 5 785.12 4.45 475 141 148 7 745.25 9.86 476 141 227 86 94.81 0.51 477 142 145 3 790.39 1.79 478 142 146 4 770.59 2.82 479 144 226 82 96.03 0.65 480 144 227 83 95.84 0.66 481 146 223 77 96.23 0.61 482 146 227 81 96.01 0.65 483 149 176 27 595.31 0.59 484 150 176 26 595.31 0.59 485 151 176 25 595.31 0.59 486 152 175 23 595.31 0.59 487 152 176 24 595.31 0.59 488 153 177 24 595.31 0.59 489 153 179 26 595.21 0.62 490 153 182 29 588.51 0.57 491 153 198 45 498.72 0.01 492 153 200 47 498.65 0.03 493 154 170 16 595.50 0.56 494 154 175 21 595.43 0.57 495 154 177 23 595.32 0.59 496 154 178 24 595.20 0.63 497 154 179 25 595.19 0.62 498 155 179 24 595.20 0.63 499 155 180 25 595.19 0.62 500 155 181 26 595.15 0.62 501 155 182 27 594.71 0.41 502 155 183 28 558.48 1.12 503 156 178 22 595.38 0.56 504 156 179 23 595.29 0.58 505 156 180 24 595.19 0.62 506 157 180 23 595.27 0.60 507 158 162 4 809.54 0.80 508 159 180 21 595.39 0.57 509 164 167 3 817.77 2.00 510 165 168 3 839.12 5.92 511 165 169 4 820.41 1.50 512 166 169 3 811.82 0.29 513 166 170 4 801.10 0.04 514 167 170 3 810.89 0.08 515 167 171 4 792.85 4.08 516 167 178 11 595.75 0.53 517 167 206 39 498.30 0.08 518 168 171 3 796.56 0.50 519 168 172 4 790.65 3.46 520 169 172 3 790.65 3.46 521 169 173 4 790.62 3.47 522 170 173 3 791.80 4.05 523 170 174 4 790.63 3.48 524 170 175 5 790.63 3.48 525 170 178 8 744.77 8.03 526 171 174 3 791.80 4.05 527 171 207 36 497.26 0.07 528 179 200 21 498.44 0.06 529 181 184 3 908.48 2.44 530 181 185 4 818.36 2.50 531 182 185 3 816.17 3.59 532 182 186 4 791.97 3.99 533 182 198 16 498.43 0.04 534 183 186 3 817.00 3.18 535 183 187 4 790.01 4.30 536 184 187 3 796.17 1.89 537 184 188 4 790.56 0.91 538 185 188 3 794.26 1.75 539 185 189 4 787.40 2.50 540 185 196 11 499.90 0.41 541 186 189 3 799.77 3.69 542 186 190 4 780.50 5.95 543 186 196 10 499.67 0.48 544 186 197 11 498.51 0.03 545 186 198 12 498.35 0.04 546 187 190 3 799.78 3.69 547 187 191 4 790.31 7.00 548 188 191 3 802.28 2.43 549 188 192 4 777.85 4.61 550 189 192 3 795.66 4.30 551 189 193 4 777.86 4.61 552 189 194 5 777.86 4.61 553 189 195 6 750.96 0.96 554 189 196 7 727.81 0.04 555 190 193 3 800.44 1.93 556 199 224 25 95.99 0.65 557 199 227 28 94.61 0.60 558 199 228 29 92.68 0.96 559 200 224 24 95.98 0.65 560 201 217 16 96.91 0.40 561 201 220 19 96.06 0.64 562 201 224 23 95.99 0.64 563 202 217 15 96.36 0.66 564 203 217 14 96.95 0.40 565 212 217 5 801.46 1.56 566 213 216 3 963.18 7.04 567 213 217 4 796.37 1.02 568 214 217 3 808.30 3.19 569 214 218 4 799.27 2.47 570 215 218 3 818.70 0.34 571 215 219 4 793.23 0.56 572 216 219 3 811.63 3.19 573 216 220 4 793.23 0.55 574 217 220 3 793.24 0.56 575 217 221 4 784.83 3.65 576 218 221 3 796.77 4.24 577 218 222 4 787.15 2.48 578 219 222 3 802.93 2.38 579 219 223 4 778.72 1.75 580 220 223 3 808.02 6.70 581 220 224 4 773.85 4.25 582 221 224 3 781.73 0.32 583 221 225 4 773.87 4.24 584 222 225 3 783.08 0.98 585 222 226 4 775.84 3.25 586 223 226 3 786.91 2.27 587 223 227 4 781.32 0.51 588 224 227 3 786.90 2.29 589 224 228 4 781.70 0.31 590 225 228 3 790.57 3.13 591 225 229 4 781.69 0.30 592 226 229 3 793.06 0.26 593 226 230 4 781.68 0.30 594 227 230 3 789.32 3.75 595 227 231 4 782.98 0.58 596 228 231 3 790.18 1.70 597 228 232 4 782.98 0.59 598 229 232 3 796.68 2.83 599 229 233 4 787.40 2.45 600 230 233 3 802.19 1.43 601 230 234 4 797.33 2.51 602 231 234 3 797.68 2.33 603 231 235 4 793.49 0.59 604 232 235 3 793.49 0.59 605 232 236 4 788.09 2.10 606 233 236 3 790.80 3.46 607 233 237 4 774.88 4.50 608 234 237 3 795.72 2.14