BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1005 1040 35 30.84 1.71 2 1005 1115 110 31.23 0.71 3 1006 1009 3 775.51 2.59 4 1006 1010 4 772.10 4.29 5 1007 1010 3 776.51 2.27 6 1007 1011 4 772.08 4.28 7 1007 1112 105 61.40 13.29 8 1008 1011 3 774.86 3.10 9 1008 1012 4 772.07 4.29 10 1008 1040 32 92.12 2.06 11 1008 1102 94 126.87 10.47 12 1008 1112 104 61.18 13.41 13 1008 1115 107 61.16 13.41 14 1008 1116 108 61.16 13.41 15 1009 1012 3 775.82 2.83 16 1009 1013 4 772.48 4.49 17 1009 1040 31 104.91 4.29 18 1009 1042 33 134.50 10.43 19 1009 1095 86 131.63 9.14 20 1010 1013 3 797.23 1.41 21 1010 1014 4 771.62 4.48 22 1011 1014 3 782.10 0.71 23 1011 1015 4 774.82 3.63 24 1011 1105 94 126.94 10.45 25 1011 1108 97 126.93 10.44 26 1011 1109 98 126.85 10.43 27 1011 1112 101 121.64 12.81 28 1012 1015 3 775.55 3.99 29 1012 1016 4 774.82 3.62 30 1012 1017 5 724.13 3.08 31 1012 1040 28 134.60 10.45 32 1012 1095 83 134.61 10.46 33 1012 1097 85 134.28 10.55 34 1012 1105 93 126.94 10.45 35 1013 1016 3 761.05 3.25 36 1013 1017 4 735.92 2.81 37 1013 1018 5 724.13 3.08 38 1013 1095 82 136.64 11.02 39 1015 1105 90 127.25 10.52 40 1017 1095 78 139.03 10.80 41 1017 1097 80 134.51 10.53 42 1017 1105 88 128.44 10.42 43 1018 1095 77 217.90 44.30 44 1019 1093 74 368.69 8.90 45 1019 1094 75 247.43 47.16 46 1019 1095 76 230.79 50.75 47 1020 1093 73 396.75 3.96 48 1020 1094 74 387.64 0.58 49 1020 1095 75 247.78 47.20 50 1020 1096 76 247.64 47.13 51 1021 1091 70 542.43 3.33 52 1021 1092 71 480.29 3.14 53 1021 1093 72 402.55 1.06 54 1022 1026 4 709.28 7.42 55 1022 1028 6 594.57 7.22 56 1022 1087 65 552.13 1.72 57 1022 1091 69 551.13 1.02 58 1022 1092 70 492.22 0.38 59 1022 1094 72 480.30 3.14 60 1022 1096 74 480.18 3.08 61 1023 1026 3 787.89 7.63 62 1023 1087 64 562.59 0.93 63 1023 1090 67 561.72 0.68 64 1023 1091 68 555.63 0.80 65 1024 1087 63 562.60 0.93 66 1024 1088 64 563.13 1.16 67 1024 1089 65 562.68 0.94 68 1024 1090 66 562.64 0.96 69 1024 1169 145 63.78 4.62 70 1025 1086 61 563.48 1.22 71 1025 1087 62 563.46 1.23 72 1026 1086 60 563.87 1.10 73 1026 1087 61 563.50 1.23 74 1027 1084 57 564.97 0.82 75 1027 1085 58 564.85 0.80 76 1027 1086 59 564.22 1.07 77 1028 1039 11 563.92 0.65 78 1028 1041 13 563.81 1.06 79 1028 1046 18 564.90 0.82 80 1028 1085 57 564.94 0.83 81 1028 1087 59 564.92 0.83 82 1028 1094 66 563.47 1.23 83 1029 1037 8 564.24 0.47 84 1029 1039 10 564.60 0.66 85 1029 1048 19 565.59 0.98 86 1029 1069 40 564.65 0.68 87 1029 1071 42 564.97 0.83 88 1029 1083 54 565.92 0.96 89 1029 1084 55 565.14 0.82 90 1029 1085 56 564.99 0.83 91 1030 1033 3 784.16 1.57 92 1030 1083 53 565.95 0.96 93 1030 1084 54 565.15 0.82 94 1031 1050 19 566.01 0.96 95 1031 1051 20 566.06 0.94 96 1031 1082 51 565.99 0.95 97 1031 1083 52 565.97 0.96 98 1031 1084 53 565.92 0.96 99 1033 1037 4 848.83 0.07 100 1036 1071 35 583.00 4.83 101 1036 1072 36 582.88 4.86 102 1036 1077 41 582.91 4.86 103 1036 1078 42 582.92 4.86 104 1036 1079 43 582.89 4.84 105 1036 1098 62 563.31 1.37 106 1036 1099 63 563.07 1.29 107 1037 1070 33 583.58 4.89 108 1037 1071 34 583.18 4.88 109 1037 1096 59 563.69 1.32 110 1037 1097 60 563.62 1.31 111 1038 1068 30 584.77 5.20 112 1038 1069 31 584.61 5.19 113 1038 1070 32 583.62 4.88 114 1038 1095 57 564.36 1.28 115 1038 1096 58 563.69 1.32 116 1038 1097 59 563.53 1.35 117 1038 1102 64 545.00 0.86 118 1039 1068 29 584.77 5.20 119 1039 1069 30 584.67 5.15 120 1039 1085 46 582.73 4.79 121 1039 1094 55 564.80 1.26 122 1039 1096 57 563.66 1.33 123 1040 1067 27 586.01 4.78 124 1040 1068 28 584.79 5.20 125 1040 1094 54 565.37 0.88 126 1040 1095 55 564.43 1.29 127 1040 1115 75 128.04 10.08 128 1041 1046 5 668.82 23.94 129 1041 1063 22 586.76 5.03 130 1041 1064 23 586.76 5.02 131 1041 1065 24 586.73 5.00 132 1041 1066 25 586.28 4.79 133 1041 1067 26 586.24 4.80 134 1041 1093 52 566.00 0.95 135 1041 1094 53 565.47 0.93 136 1042 1065 23 586.77 5.02 137 1042 1066 24 586.28 4.78 138 1042 1093 51 566.09 0.98 139 1042 1094 52 565.98 0.95 140 1042 1095 53 565.98 0.95 141 1043 1065 22 586.78 5.02 142 1043 1092 49 566.12 0.97 143 1043 1093 50 566.08 0.99 144 1044 1064 20 587.20 4.99 145 1044 1065 21 587.14 4.96 146 1044 1089 45 582.59 4.73 147 1044 1092 48 566.09 0.96 148 1045 1063 18 587.51 5.08 149 1045 1064 19 587.20 4.99 150 1045 1087 42 583.15 4.73 151 1045 1088 43 583.02 4.69 152 1045 1089 44 582.71 4.79 153 1045 1154 109 62.30 3.96 154 1045 1166 121 63.13 4.37 155 1046 1062 16 588.61 5.38 156 1046 1063 17 587.64 5.01 157 1046 1085 39 584.29 5.15 158 1046 1086 40 584.14 5.18 159 1046 1087 41 583.15 4.73 160 1046 1088 42 583.12 4.73 161 1047 1059 12 592.41 5.02 162 1047 1061 14 589.85 5.36 163 1047 1062 15 588.70 5.34 164 1047 1084 37 585.24 4.90 165 1047 1085 38 584.33 5.13 166 1047 1086 39 584.18 5.17 167 1047 1088 41 583.15 4.73 168 1047 1173 126 64.23 4.59 169 1048 1059 11 592.63 4.96 170 1048 1060 12 592.39 5.03 171 1048 1061 13 589.92 5.34 172 1048 1063 15 589.40 5.40 173 1048 1083 35 585.38 4.91 174 1048 1084 36 585.24 4.90 175 1048 1085 37 584.58 5.20 176 1049 1057 8 593.01 5.00 177 1049 1058 9 592.70 4.98 178 1049 1059 10 592.62 4.96 179 1049 1060 11 592.41 5.02 180 1049 1083 34 585.40 4.91 181 1049 1084 35 585.31 4.93 182 1050 1057 7 593.13 5.05 183 1050 1058 8 592.84 5.03 184 1050 1060 10 592.71 4.98 185 1050 1082 32 585.76 5.04 186 1050 1083 33 585.52 4.97 187 1051 1055 4 607.50 0.20 188 1051 1056 5 594.57 5.72 189 1051 1057 6 593.13 5.06 190 1051 1084 33 585.75 5.04 191 1052 1055 3 743.96 4.29 192 1052 1056 4 595.85 6.02 193 1052 1058 6 594.57 5.72 194 1059 1062 3 905.24 0.48 195 1060 1083 23 585.62 5.00 196 1061 1126 65 127.50 10.15 197 1062 1088 26 583.20 4.74 198 1062 1173 111 64.34 4.65 199 1062 1174 112 64.39 4.58 200 1063 1067 4 766.00 0.21 201 1063 1069 6 628.12 6.91 202 1063 1085 22 584.36 5.12 203 1063 1123 60 127.55 10.14 204 1064 1067 3 812.89 0.58 205 1064 1153 89 61.76 3.69 206 1064 1154 90 61.77 3.69 207 1067 1119 52 127.62 10.11 208 1067 1122 55 127.61 10.11 209 1067 1123 56 127.55 10.13 210 1068 1115 47 128.05 10.08 211 1068 1116 48 127.78 10.12 212 1068 1119 51 127.60 10.11 213 1069 1085 16 582.72 4.78 214 1070 1074 4 800.37 2.37 215 1070 1080 10 589.75 3.21 216 1070 1099 29 562.92 1.36 217 1070 1102 32 548.18 2.09 218 1071 1079 8 589.77 3.20 219 1071 1080 9 589.75 3.21 220 1072 1078 6 589.94 3.14 221 1072 1079 7 589.77 3.20 222 1072 1080 8 589.75 3.21 223 1072 1099 27 563.26 1.39 224 1073 1076 3 787.43 1.27 225 1073 1077 4 646.45 16.92 226 1073 1078 5 646.27 17.01 227 1073 1080 7 589.93 3.15 228 1073 1099 26 563.31 1.36 229 1074 1077 3 765.99 2.29 230 1074 1099 25 563.31 1.36 231 1074 1102 28 562.64 1.39 232 1074 1103 29 549.94 2.08 233 1077 1099 22 563.29 1.37 234 1077 1100 23 563.28 1.37 235 1077 1103 26 561.48 1.54 236 1079 1082 3 860.08 3.18 237 1086 1173 87 64.19 4.58 238 1087 1091 4 776.01 4.41 239 1087 1092 5 596.88 3.63 240 1088 1170 82 63.57 4.59 241 1088 1173 85 64.04 4.63 242 1097 1101 4 756.64 0.69 243 1097 1102 5 755.09 1.46 244 1097 1105 8 743.23 0.64 245 1098 1101 3 782.20 3.17 246 1098 1102 4 758.46 1.59 247 1099 1102 3 769.33 1.76 248 1099 1103 4 758.47 1.59 249 1100 1103 3 772.87 7.83 250 1100 1104 4 758.46 1.60 251 1101 1104 3 768.34 2.54 252 1101 1105 4 759.43 1.11 253 1102 1105 3 772.48 5.39 254 1102 1106 4 761.85 0.12 255 1103 1106 3 779.01 2.12 256 1103 1107 4 765.33 1.86 257 1104 1107 3 770.10 0.53 258 1104 1108 4 765.32 1.86 259 1106 1109 3 769.09 3.62 260 1106 1110 4 732.68 1.95 261 1106 1112 6 667.61 3.91 262 1106 1113 7 605.19 1.34 263 1106 1116 10 606.38 1.94 264 1107 1110 3 790.05 0.84 265 1109 1112 3 786.46 1.65 266 1109 1113 4 767.62 4.16 267 1110 1113 3 768.21 3.87 268 1110 1114 4 762.19 1.45 269 1111 1114 3 772.50 3.71 270 1111 1115 4 764.12 0.48 271 1112 1115 3 764.16 0.49 272 1112 1116 4 762.25 0.48 273 1113 1116 3 772.37 1.41 274 1113 1117 4 762.25 0.48 275 1114 1117 3 766.86 1.82 276 1114 1118 4 768.37 3.56 277 1115 1118 3 770.36 2.57 278 1115 1119 4 766.86 1.82 279 1116 1119 3 773.00 1.25 280 1116 1120 4 766.85 1.82 281 1117 1120 3 768.89 2.83 282 1117 1121 4 764.77 2.85 283 1118 1121 3 766.79 3.89 284 1118 1122 4 764.72 2.87 285 1119 1122 3 764.75 2.87 286 1119 1123 4 764.75 2.87 287 1120 1123 3 769.98 3.00 288 1120 1124 4 764.70 2.90 289 1121 1124 3 767.22 1.62 290 1121 1125 4 764.68 2.89 291 1122 1125 3 775.89 1.19 292 1122 1126 4 760.10 1.98 293 1122 1224 93 67.02 2.65 294 1122 1228 97 64.31 3.17 295 1123 1126 3 775.50 1.38 296 1123 1127 4 760.08 1.98 297 1124 1127 3 774.95 1.09 298 1124 1128 4 766.05 2.17 299 1125 1128 3 776.84 1.50 300 1125 1129 4 766.05 2.17 301 1125 1224 90 67.28 2.69 302 1126 1129 3 775.59 0.86 303 1126 1130 4 762.30 0.29 304 1126 1149 23 67.47 2.77 305 1126 1221 86 67.32 2.70 306 1126 1224 89 67.30 2.69 307 1127 1130 3 762.31 0.29 308 1127 1131 4 762.32 0.29 309 1128 1131 3 764.88 0.50 310 1128 1132 4 763.38 0.24 311 1129 1132 3 773.33 1.68 312 1129 1133 4 770.27 3.20 313 1129 1220 82 95.91 11.60 314 1129 1221 83 67.81 2.46 315 1129 1224 86 67.85 2.49 316 1130 1133 3 782.23 2.41 317 1130 1134 4 769.22 3.73 318 1131 1134 3 776.05 3.04 319 1131 1135 4 768.59 4.04 320 1132 1135 3 770.19 3.63 321 1132 1136 4 768.98 4.24 322 1132 1220 79 99.40 10.51 323 1133 1136 3 768.99 4.23 324 1133 1137 4 760.71 0.12 325 1133 1141 8 217.89 45.95 326 1133 1218 76 99.42 10.50 327 1133 1219 77 99.42 10.50 328 1133 1220 78 99.41 10.50 329 1133 1221 79 99.41 10.50 330 1134 1137 3 772.39 2.53 331 1134 1138 4 707.77 7.24 332 1134 1139 5 740.95 7.17 333 1134 1142 8 500.66 62.08 334 1137 1140 3 769.05 2.33 335 1137 1141 4 689.99 7.96 336 1137 1217 71 190.56 1.35 337 1137 1219 73 199.88 3.32 338 1138 1141 3 769.63 4.02 339 1138 1142 4 760.87 0.36 340 1139 1142 3 760.42 0.14 341 1139 1143 4 749.59 3.02 342 1139 1189 41 283.39 6.25 343 1140 1144 4 758.95 0.82 344 1140 1189 40 283.60 6.15 345 1140 1217 68 278.13 6.88 346 1141 1144 3 772.79 1.04 347 1141 1145 4 759.79 0.69 348 1141 1217 67 278.12 6.88 349 1142 1145 3 771.41 0.36 350 1142 1146 4 759.20 0.94 351 1143 1146 3 771.12 1.27 352 1143 1147 4 765.49 4.09 353 1143 1186 34 284.99 6.60 354 1143 1189 37 284.86 6.62 355 1143 1190 38 284.83 6.63 356 1143 1193 41 284.83 6.63 357 1144 1147 3 770.76 1.46 358 1144 1148 4 759.06 1.05 359 1144 1213 60 283.22 6.39 360 1144 1214 61 283.00 6.33 361 1144 1217 64 282.28 6.25 362 1144 1218 65 282.17 6.25 363 1145 1148 3 771.56 5.20 364 1145 1149 4 768.88 2.44 365 1145 1218 64 282.19 6.27 366 1145 1221 67 282.12 6.25 367 1146 1149 3 789.99 3.18 368 1146 1150 4 768.89 2.45 369 1147 1150 3 776.96 1.89 370 1147 1151 4 768.88 2.44 371 1147 1190 34 285.06 6.59 372 1147 1193 37 285.04 6.59 373 1147 1199 43 284.94 6.59 374 1147 1214 58 283.31 6.45 375 1148 1151 3 777.88 2.04 376 1148 1152 4 768.89 2.44 377 1148 1211 54 284.05 6.38 378 1148 1214 57 283.31 6.45 379 1148 1215 58 283.31 6.45 380 1148 1218 61 282.32 6.25 381 1148 1221 64 282.16 6.27 382 1148 1222 65 282.14 6.25 383 1149 1152 3 769.68 4.15 384 1149 1153 4 754.11 3.37 385 1149 1221 63 282.16 6.27 386 1149 1225 67 279.21 5.30 387 1150 1153 3 776.32 0.83 388 1150 1154 4 745.98 0.47 389 1150 1155 5 716.49 8.63 390 1150 1164 14 285.76 6.88 391 1150 1166 16 287.19 6.17 392 1150 1171 21 286.97 6.07 393 1151 1155 4 670.17 4.61 394 1151 1162 11 285.75 6.90 395 1151 1164 13 285.76 6.88 396 1151 1208 48 285.11 6.58 397 1151 1211 51 284.82 6.53 398 1151 1214 54 284.49 6.60 399 1151 1236 76 159.64 24.36 400 1152 1211 50 284.82 6.53 401 1152 1215 54 284.64 6.62 402 1152 1222 61 282.21 6.26 403 1152 1225 64 279.36 5.36 404 1152 1232 71 161.28 24.82 405 1152 1233 72 161.27 24.83 406 1152 1236 75 159.50 24.29 407 1152 1237 76 159.66 24.21 408 1153 1225 63 279.66 5.24 409 1153 1232 70 161.44 24.74 410 1154 1165 11 287.53 6.02 411 1154 1166 12 287.51 6.03 412 1155 1162 7 288.49 5.61 413 1155 1163 8 287.97 5.87 414 1155 1164 9 287.81 5.92 415 1156 1162 6 288.54 5.61 416 1156 1163 7 288.49 5.61 417 1156 1165 9 287.85 5.92 418 1157 1161 4 426.96 12.63 419 1157 1162 5 288.55 5.60 420 1157 1236 70 160.69 24.71 421 1158 1161 3 749.28 0.69 422 1158 1163 5 520.99 37.02 423 1158 1207 40 287.14 5.88 424 1160 1202 33 287.23 5.86 425 1160 1207 38 287.12 5.85 426 1160 1209 40 286.86 5.94 427 1161 1208 38 286.79 5.96 428 1162 1206 35 287.09 5.90 429 1162 1207 36 286.77 5.96 430 1162 1208 37 286.77 5.96 431 1162 1211 40 286.42 6.07 432 1162 1236 65 160.66 24.71 433 1163 1205 33 287.08 5.90 434 1163 1206 34 286.85 5.92 435 1163 1207 35 286.78 5.95 436 1164 1171 7 652.80 5.49 437 1164 1206 33 286.81 5.93 438 1164 1208 35 286.78 5.94 439 1166 1171 5 683.97 1.77 440 1166 1174 8 639.62 1.14 441 1167 1170 3 764.59 0.54 442 1167 1171 4 756.75 3.81 443 1168 1171 3 782.80 5.74 444 1168 1204 27 287.19 5.80 445 1169 1172 3 775.01 0.24 446 1170 1173 3 773.43 1.03 447 1170 1174 4 773.43 1.03 448 1171 1174 3 768.48 1.45 449 1171 1206 26 287.23 5.83 450 1184 1187 3 786.43 4.88 451 1185 1188 3 766.39 5.13 452 1185 1189 4 763.81 0.84 453 1186 1189 3 769.48 1.81 454 1186 1190 4 756.77 2.68 455 1187 1190 3 762.61 1.02 456 1187 1191 4 756.75 2.69 457 1188 1191 3 762.02 1.32 458 1188 1192 4 732.65 7.78 459 1189 1192 3 753.79 2.79 460 1189 1193 4 732.65 7.78 461 1190 1193 3 764.72 2.68 462 1190 1194 4 751.53 3.92 463 1190 1199 9 368.88 19.64 464 1190 1206 16 308.89 4.13 465 1191 1194 3 764.18 0.17 466 1191 1195 4 750.82 4.27 467 1192 1195 3 767.83 1.12 468 1192 1196 4 750.82 4.27 469 1192 1213 21 308.39 4.15 470 1193 1196 3 758.08 2.63 471 1193 1197 4 730.26 4.78 472 1193 1198 5 630.00 6.63 473 1193 1199 6 616.33 10.18 474 1193 1213 20 308.38 4.13 475 1194 1197 3 737.65 5.78 476 1194 1199 5 475.08 62.61 477 1194 1201 7 367.95 20.10 478 1196 1213 17 308.41 4.14 479 1197 1209 12 308.85 4.16 480 1197 1210 13 308.83 4.16 481 1198 1208 10 308.88 4.17 482 1198 1209 11 308.84 4.17 483 1198 1210 12 308.73 4.17 484 1198 1213 15 308.72 4.17 485 1198 1214 16 308.69 4.16 486 1199 1206 7 310.03 4.41 487 1199 1207 8 309.36 4.15 488 1199 1208 9 308.86 4.19 489 1200 1206 6 317.56 5.58 490 1200 1207 7 309.94 4.40 491 1200 1209 9 309.94 4.42 492 1201 1204 3 813.40 1.89 493 1201 1205 4 727.09 7.92 494 1201 1206 5 330.04 0.65 495 1202 1205 3 776.42 0.14 496 1202 1207 5 708.37 17.29 497 1203 1207 4 824.11 2.58 498 1208 1211 3 799.82 6.70 499 1208 1214 6 736.00 11.33 500 1210 1213 3 771.47 3.17 501 1210 1214 4 760.01 0.97 502 1211 1214 3 767.97 0.68 503 1211 1215 4 757.70 2.12 504 1211 1236 25 159.26 24.34 505 1212 1215 3 768.88 1.14 506 1212 1216 4 757.58 2.18 507 1213 1216 3 768.88 1.14 508 1213 1217 4 764.26 1.17 509 1214 1217 3 778.74 3.78 510 1214 1218 4 757.11 2.41 511 1215 1218 3 773.08 3.25 512 1215 1219 4 737.30 10.89 513 1215 1222 7 521.00 62.00 514 1215 1233 18 217.84 53.12 515 1215 1237 22 158.22 24.60 516 1216 1219 3 782.19 4.24 517 1218 1221 3 772.97 1.60 518 1218 1222 4 744.57 7.23 519 1219 1222 3 772.88 1.65 520 1219 1223 4 763.71 2.93 521 1220 1223 3 763.72 2.93 522 1220 1224 4 763.72 2.93 523 1221 1224 3 763.72 2.93 524 1221 1225 4 763.73 2.92 525 1222 1225 3 773.49 4.50 526 1222 1226 4 737.14 10.38 527 1222 1233 11 338.26 0.38 528 1223 1226 3 773.06 3.06 529 1223 1227 4 753.71 2.10 530 1224 1227 3 766.98 2.32 531 1224 1228 4 736.91 10.50 532 1225 1228 3 767.87 2.77 533 1225 1229 4 715.63 11.10 534 1225 1232 7 627.58 1.93 535 1225 1233 8 352.22 7.35 536 1226 1230 4 620.58 1.56 537 1226 1233 7 352.01 6.49 538 1229 1232 3 785.21 0.46 539 1229 1233 4 748.70 1.69 540 1230 1233 3 777.75 3.27 541 1230 1234 4 756.01 1.97 542 1231 1234 3 778.18 0.84 543 1231 1235 4 760.76 0.40 544 1232 1235 3 777.62 1.12 545 1233 1236 3 787.82 1.67 546 1233 1237 4 760.11 0.07 547 1234 1237 3 759.91 0.10