BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 6 3 804.88 0.76 2 3 7 4 465.50 63.50 3 3 8 5 441.46 75.51 4 3 76 73 24.58 2.65 5 3 80 77 27.04 1.42 6 4 7 3 812.26 1.82 7 4 43 39 50.54 0.35 8 4 76 72 39.80 1.65 9 5 43 38 50.52 0.35 10 5 44 39 51.17 0.03 11 5 46 41 49.66 0.18 12 5 72 67 45.38 1.12 13 5 76 71 40.02 1.56 14 6 40 34 81.77 15.26 15 6 41 35 50.51 0.35 16 6 46 40 50.31 0.45 17 6 61 55 48.42 0.32 18 6 73 67 47.70 0.04 19 6 76 70 45.39 1.12 20 6 80 74 115.06 2.98 21 7 41 34 75.90 12.22 22 7 43 36 57.73 1.74 23 8 12 4 786.30 0.59 24 8 40 32 110.42 0.98 25 8 80 72 155.72 0.94 26 9 12 3 789.75 0.74 27 9 13 4 786.30 0.59 28 9 80 71 155.73 0.94 29 10 13 3 804.56 6.13 30 10 80 70 155.72 0.94 31 12 40 28 233.00 0.47 32 12 50 38 240.99 3.91 33 13 17 4 806.69 1.15 34 13 40 27 240.96 3.90 35 13 50 37 252.40 1.79 36 13 59 46 242.65 3.08 37 13 77 64 211.04 0.46 38 13 80 67 211.49 0.67 39 14 17 3 839.41 2.53 40 14 50 36 267.75 0.39 41 14 57 43 286.19 1.59 42 14 58 44 285.94 1.54 43 14 59 45 268.26 2.41 44 14 82 68 211.50 0.67 45 15 81 66 211.89 0.67 46 15 82 67 211.52 0.68 47 15 83 68 211.49 0.67 48 15 84 69 211.47 0.69 49 16 56 40 286.93 1.42 50 16 57 41 286.70 1.40 51 16 58 42 286.29 1.56 52 16 59 43 286.29 1.56 53 16 83 67 211.55 0.68 54 16 84 68 211.50 0.68 55 16 232 216 70.92 0.04 56 16 233 217 70.88 0.04 57 16 235 219 54.70 0.01 58 17 59 42 286.31 1.57 59 17 61 44 286.26 1.57 60 17 77 60 212.41 0.57 61 17 82 65 212.34 0.58 62 17 84 67 211.56 0.67 63 17 85 68 211.50 0.68 64 17 86 69 211.49 0.67 65 18 29 11 287.06 1.39 66 18 58 40 286.88 1.45 67 18 59 41 286.51 1.52 68 18 60 42 286.32 1.58 69 18 61 43 286.31 1.58 70 18 86 68 211.51 0.68 71 18 88 70 211.49 0.68 72 18 225 207 97.35 0.41 73 18 228 210 92.09 1.03 74 18 229 211 92.06 1.02 75 18 232 214 70.98 0.04 76 19 61 42 286.31 1.58 77 19 63 44 286.17 1.59 78 19 74 55 213.72 1.05 79 19 77 58 213.69 1.02 80 19 85 66 212.22 0.59 81 19 86 67 211.55 0.67 82 19 87 68 211.49 0.67 83 19 88 69 211.50 0.68 84 19 185 166 211.97 0.62 85 20 25 5 748.94 0.31 86 20 26 6 523.42 89.88 87 20 29 9 518.02 92.58 88 20 60 40 286.87 1.44 89 20 61 41 286.62 1.55 90 20 62 42 286.30 1.58 91 20 63 43 286.26 1.60 92 20 88 68 211.52 0.68 93 21 25 4 807.25 4.62 94 21 62 41 286.37 1.58 95 21 63 42 286.27 1.60 96 21 64 43 286.19 1.59 97 21 87 66 212.36 0.56 98 22 25 3 811.52 2.48 99 22 26 4 800.81 2.02 100 22 62 40 286.86 1.44 101 23 26 3 800.82 2.02 102 23 27 4 800.81 2.02 103 23 45 22 286.85 1.44 104 23 47 24 286.85 1.44 105 23 62 39 286.86 1.44 106 24 27 3 800.85 2.04 107 24 28 4 788.90 3.94 108 25 28 3 798.34 3.32 109 25 29 4 782.89 0.99 110 25 88 63 212.05 0.53 111 25 89 64 211.75 0.62 112 25 90 65 211.59 0.68 113 25 221 196 99.30 1.01 114 25 224 199 98.84 0.79 115 26 29 3 799.12 3.71 116 26 30 4 786.10 2.60 117 26 49 23 287.20 1.34 118 26 60 34 287.18 1.35 119 26 62 36 286.83 1.45 120 27 30 3 794.38 3.73 121 27 31 4 783.34 4.00 122 27 47 20 286.90 1.42 123 27 49 22 287.22 1.33 124 28 31 3 805.76 1.07 125 28 32 4 781.66 4.84 126 28 33 5 781.66 4.84 127 28 218 190 99.53 0.96 128 28 221 193 99.42 1.02 129 28 222 194 99.28 1.01 130 28 225 197 98.59 0.73 131 29 32 3 795.36 6.28 132 29 33 4 777.24 7.05 133 29 34 5 777.24 7.05 134 29 60 31 287.21 1.37 135 29 88 59 211.62 0.65 136 29 225 196 97.36 0.40 137 30 34 4 760.15 1.48 138 30 37 7 300.55 3.17 139 30 49 19 287.24 1.35 140 31 37 6 517.36 110.55 141 33 53 20 329.81 1.28 142 33 222 189 99.27 1.00 143 33 225 192 97.67 0.27 144 33 226 193 97.20 0.48 145 33 229 196 92.16 1.02 146 34 37 3 815.21 5.23 147 34 51 17 329.76 1.27 148 34 52 18 329.82 1.28 149 34 53 19 329.81 1.28 150 34 58 24 329.76 1.27 151 34 60 26 326.83 0.04 152 34 229 195 92.14 1.02 153 35 51 16 329.86 1.27 154 35 52 17 329.86 1.27 155 36 51 15 329.89 1.28 156 37 50 13 330.02 1.32 157 37 51 14 329.91 1.27 158 38 49 11 330.44 1.51 159 38 50 12 330.44 1.51 160 39 42 3 878.06 3.46 161 39 47 8 330.94 1.39 162 39 48 9 330.77 1.34 163 39 49 10 330.48 1.50 164 40 48 8 330.89 1.40 165 40 49 9 330.82 1.42 166 40 50 10 330.82 1.43 167 40 59 19 330.69 1.49 168 40 61 21 330.57 1.51 169 41 46 5 533.35 90.48 170 41 47 6 331.04 1.42 171 41 48 7 330.93 1.40 172 42 45 3 782.51 5.04 173 42 47 5 331.06 1.43 174 43 46 3 815.66 2.74 175 45 62 17 330.62 1.49 176 46 61 15 330.63 1.49 177 46 62 16 330.57 1.49 178 46 63 17 330.56 1.50 179 46 64 18 330.28 1.38 180 46 73 27 213.81 1.04 181 47 61 14 330.62 1.50 182 47 62 15 330.57 1.50 183 48 60 12 330.67 1.49 184 48 61 13 330.52 1.49 185 49 59 10 330.78 1.51 186 49 60 11 330.69 1.50 187 50 58 8 330.84 1.53 188 50 59 9 330.78 1.51 189 51 57 6 330.87 1.52 190 51 58 7 330.85 1.53 191 52 55 3 795.10 7.19 192 52 56 4 521.86 96.79 193 52 57 5 330.88 1.52 194 53 56 3 784.02 1.66 195 53 58 5 516.12 94.11 196 53 229 176 92.14 1.02 197 53 230 177 92.12 1.02 198 53 233 180 70.97 0.04 199 54 230 176 92.14 1.02 200 54 233 179 70.98 0.04 201 56 233 177 70.87 0.04 202 58 229 171 91.97 1.06 203 58 232 174 70.95 0.06 204 58 233 175 70.92 0.06 205 61 73 12 213.79 1.04 206 61 77 16 213.38 1.03 207 62 73 11 213.81 1.04 208 63 69 6 251.15 19.70 209 63 70 7 213.77 1.05 210 63 73 10 213.81 1.04 211 64 69 5 559.48 82.25 212 64 181 117 213.50 0.98 213 65 69 4 789.80 2.94 214 65 181 116 213.52 0.99 215 66 69 3 808.91 4.83 216 66 70 4 789.81 2.94 217 66 180 114 213.25 0.92 218 66 181 115 213.59 1.02 219 67 70 3 790.47 2.62 220 67 71 4 779.93 1.99 221 67 113 46 213.63 1.02 222 67 115 48 213.91 0.91 223 67 157 90 213.63 0.99 224 67 180 113 213.58 1.02 225 67 181 114 213.61 1.02 226 67 183 116 213.64 1.02 227 67 184 117 213.64 1.02 228 68 71 3 807.68 5.94 229 68 72 4 785.16 0.04 230 68 115 47 213.93 0.90 231 69 72 3 817.71 0.92 232 69 73 4 795.65 5.28 233 70 73 3 812.88 3.33 234 70 74 4 799.62 3.30 235 70 181 111 213.58 0.89 236 71 74 3 800.56 2.83 237 71 75 4 788.01 1.39 238 71 115 44 214.12 0.80 239 71 119 48 215.34 0.89 240 71 184 113 213.84 0.89 241 71 188 117 214.53 0.70 242 72 75 3 810.70 2.65 243 72 76 4 791.90 0.56 244 73 76 3 810.35 2.90 245 73 77 4 792.82 3.77 246 74 77 3 794.73 4.73 247 74 78 4 794.73 4.73 248 74 85 11 213.79 0.86 249 74 185 111 213.80 0.85 250 74 188 114 215.31 0.94 251 75 78 3 804.40 0.10 252 75 79 4 794.73 4.73 253 75 119 44 216.19 1.11 254 75 188 113 215.53 0.83 255 75 192 117 278.25 29.39 256 76 79 3 803.55 0.33 257 76 80 4 792.39 1.22 258 77 80 3 798.75 1.96 259 77 81 4 787.71 3.56 260 77 82 5 745.03 0.41 261 77 85 8 278.26 29.36 262 78 81 3 795.70 5.62 263 78 82 4 736.92 3.57 264 78 192 114 354.93 2.59 265 78 194 116 370.30 2.07 266 82 85 3 845.14 1.05 267 82 194 112 495.48 1.54 268 83 194 111 495.48 1.54 269 83 195 112 495.48 1.54 270 83 235 152 60.29 2.79 271 84 133 49 495.48 1.54 272 84 195 111 495.48 1.54 273 84 232 148 70.96 0.06 274 84 235 151 53.88 0.40 275 85 185 100 495.53 1.53 276 85 194 109 495.60 1.53 277 85 195 110 495.52 1.53 278 85 196 111 495.49 1.54 279 85 197 112 495.49 1.54 280 86 133 47 495.53 1.52 281 86 137 51 495.49 1.54 282 86 197 111 495.49 1.54 283 86 198 112 495.48 1.54 284 86 199 113 495.48 1.54 285 86 228 142 92.09 1.03 286 86 232 146 70.98 0.04 287 87 179 92 495.52 1.53 288 87 181 94 495.56 1.53 289 87 182 95 495.53 1.53 290 87 185 98 495.62 1.53 291 87 197 110 495.53 1.53 292 87 198 111 495.49 1.54 293 87 199 112 495.49 1.54 294 87 200 113 495.49 1.54 295 88 199 111 495.50 1.54 296 88 224 136 98.69 0.86 297 88 225 137 97.16 0.51 298 88 228 140 92.09 1.03 299 89 199 110 495.54 1.53 300 89 200 111 495.49 1.54 301 89 201 112 495.49 1.54 302 89 224 135 98.83 0.79 303 90 179 89 495.59 1.53 304 90 200 110 495.52 1.53 305 90 201 111 495.49 1.54 306 90 203 113 493.75 0.98 307 90 217 127 99.80 0.96 308 90 221 131 99.41 0.99 309 91 177 86 495.57 1.53 310 91 178 87 495.57 1.53 311 91 179 88 495.59 1.53 312 91 200 109 495.59 1.53 313 91 201 110 495.53 1.53 314 91 202 111 495.45 1.53 315 91 203 112 495.43 1.52 316 92 177 85 495.59 1.53 317 92 178 86 495.61 1.52 318 92 204 112 495.06 1.58 319 92 206 114 418.19 2.07 320 93 98 5 495.50 1.54 321 93 176 83 495.63 1.53 322 93 177 84 495.62 1.52 323 93 202 109 495.58 1.53 324 93 204 111 495.49 1.54 325 93 205 112 435.57 0.23 326 93 206 113 435.03 0.04 327 94 167 73 495.56 1.53 328 94 168 74 495.56 1.53 329 94 171 77 495.56 1.53 330 94 175 81 495.66 1.51 331 94 176 82 495.67 1.51 332 94 177 83 495.68 1.51 333 94 178 84 495.73 1.51 334 94 206 112 435.05 0.04 335 95 98 3 818.86 2.39 336 95 168 73 495.59 1.53 337 95 171 76 495.63 1.53 338 96 168 72 495.73 1.52 339 96 205 109 495.52 1.53 340 97 149 52 495.80 1.52 341 98 102 4 784.01 1.10 342 98 104 6 714.46 2.33 343 98 149 51 495.72 1.51 344 98 176 78 495.78 1.49 345 98 177 79 495.86 1.52 346 98 202 104 495.78 1.51 347 98 205 107 495.55 1.55 348 99 102 3 815.66 0.89 349 99 202 103 495.81 1.50 350 99 205 106 495.68 1.50 351 100 202 102 495.80 1.51 352 100 203 103 495.76 1.51 353 100 204 104 495.75 1.51 354 100 205 105 495.74 1.50 355 100 212 112 100.16 0.94 356 100 216 116 100.09 0.95 357 101 202 101 495.82 1.50 358 101 216 115 100.02 0.96 359 101 220 119 99.67 0.96 360 102 147 45 691.14 2.69 361 102 148 46 691.03 2.73 362 102 149 47 691.00 2.72 363 102 201 99 496.03 1.50 364 102 202 100 495.88 1.52 365 103 146 43 691.26 2.67 366 103 147 44 691.23 2.67 367 103 148 45 691.03 2.73 368 103 149 46 691.02 2.73 369 103 199 96 496.74 1.31 370 103 200 97 496.11 1.52 371 103 201 98 496.05 1.51 372 103 220 117 99.66 0.96 373 103 223 120 99.62 0.94 374 103 224 121 99.50 1.00 375 103 227 124 98.98 0.74 376 104 149 45 691.03 2.73 377 104 153 49 627.83 2.87 378 104 176 72 557.22 0.38 379 104 177 73 549.63 0.73 380 104 199 95 496.74 1.31 381 104 200 96 496.45 1.44 382 104 202 98 496.12 1.52 383 105 134 29 691.50 2.71 384 105 137 32 691.43 2.65 385 105 138 33 691.38 2.68 386 105 149 44 691.25 2.66 387 105 150 45 691.06 2.71 388 105 151 46 691.06 2.71 389 105 198 93 498.11 1.28 390 105 199 94 496.77 1.30 391 106 129 23 692.13 2.69 392 106 134 28 691.56 2.68 393 106 151 45 691.08 2.71 394 106 153 47 685.60 0.98 395 106 186 80 547.96 0.84 396 106 198 92 498.15 1.29 397 106 200 94 497.85 1.38 398 107 128 21 692.20 2.66 399 107 129 22 692.13 2.69 400 107 134 27 691.60 2.67 401 107 150 43 691.31 2.67 402 107 151 44 691.12 2.69 403 108 127 19 692.23 2.66 404 108 128 20 692.20 2.66 405 108 129 21 692.20 2.65 406 108 151 43 691.34 2.67 407 108 152 44 691.13 2.70 408 108 153 45 691.13 2.70 409 108 186 78 548.82 0.66 410 109 127 18 692.23 2.65 411 109 152 43 691.32 2.67 412 109 153 44 691.17 2.69 413 109 155 46 691.08 2.71 414 109 182 73 553.44 1.39 415 109 183 74 551.75 1.49 416 109 186 77 548.91 0.66 417 110 152 42 691.37 2.66 418 110 153 43 691.35 2.66 419 110 154 44 691.12 2.70 420 110 155 45 691.12 2.71 421 110 170 60 572.08 0.10 422 110 175 65 572.02 0.12 423 111 116 5 735.79 4.59 424 111 127 16 692.34 2.69 425 111 183 72 553.79 1.26 426 112 154 42 691.37 2.66 427 112 155 43 691.29 2.66 428 112 156 44 691.14 2.69 429 112 170 58 572.21 0.14 430 112 178 66 572.02 0.11 431 113 116 3 823.08 1.06 432 113 117 4 785.76 1.56 433 113 155 42 691.37 2.66 434 113 157 44 691.15 2.69 435 113 183 70 571.22 0.23 436 114 117 3 792.18 4.77 437 114 118 4 793.96 3.88 438 114 157 43 691.24 2.69 439 115 118 3 813.03 1.54 440 115 119 4 793.97 3.89 441 115 184 69 570.90 0.00 442 116 119 3 801.15 7.48 443 116 120 4 793.96 3.88 444 116 183 67 570.98 0.03 445 116 184 68 569.82 0.20 446 116 187 71 555.09 1.96 447 117 120 3 800.80 7.29 448 117 121 4 782.96 7.71 449 117 122 5 706.43 1.25 450 118 121 3 787.83 5.29 451 119 187 68 555.62 1.74 452 119 188 69 555.18 1.93 453 119 191 72 549.77 0.86 454 119 192 73 549.77 0.86 455 120 123 3 795.96 3.48 456 120 125 5 709.29 5.46 457 120 187 67 555.60 1.73 458 125 187 62 549.83 0.47 459 125 190 65 549.29 0.67 460 127 183 56 551.76 1.48 461 127 186 59 549.04 0.78 462 128 186 58 548.76 0.65 463 128 190 62 539.98 0.45 464 129 134 5 762.68 7.24 465 129 186 57 548.32 0.74 466 129 190 61 539.95 0.46 467 129 196 67 498.48 1.25 468 129 197 68 498.32 1.27 469 130 133 3 799.75 2.22 470 130 134 4 782.22 6.96 471 130 190 60 539.97 0.45 472 130 195 65 498.70 1.31 473 130 196 66 498.67 1.32 474 131 134 3 794.68 0.73 475 131 135 4 782.23 6.96 476 131 190 59 539.99 0.45 477 132 135 3 801.20 3.21 478 132 136 4 782.25 6.97 479 132 195 63 498.80 1.34 480 132 235 103 70.95 0.06 481 133 136 3 795.47 0.36 482 133 137 4 781.28 7.46 483 133 195 62 498.78 1.34 484 133 196 63 498.74 1.33 485 133 197 64 498.66 1.33 486 133 235 102 70.45 0.10 487 134 137 3 803.48 3.61 488 134 138 4 783.35 6.45 489 134 150 16 691.39 2.67 490 135 138 3 794.20 1.02 491 135 139 4 781.29 7.45 492 136 139 3 797.24 0.50 493 136 140 4 771.78 3.04 494 136 231 95 86.22 0.18 495 136 234 98 74.42 1.66 496 136 235 99 70.98 0.04 497 137 140 3 797.92 0.39 498 137 141 4 775.97 0.97 499 137 197 60 498.58 1.36 500 137 198 61 498.58 1.36 501 137 199 62 498.51 1.34 502 137 227 90 93.91 0.62 503 137 228 91 92.16 1.02 504 137 231 94 80.70 0.11 505 138 141 3 807.15 4.22 506 138 142 4 787.81 1.40 507 138 148 10 691.64 2.71 508 138 150 12 691.38 2.67 509 139 142 3 791.95 1.64 510 139 143 4 769.45 4.28 511 140 143 3 784.69 0.15 512 140 144 4 765.13 6.43 513 140 227 87 97.56 0.36 514 140 230 90 92.11 1.02 515 140 231 91 92.09 1.03 516 140 234 94 91.44 1.21 517 141 144 3 790.40 2.21 518 141 145 4 764.73 6.62 519 141 146 5 753.47 1.05 520 141 148 7 692.25 2.50 521 141 227 86 98.13 0.60 522 142 145 3 786.36 10.50 523 142 148 6 708.65 10.65 524 144 226 82 99.59 0.95 525 144 227 83 98.92 0.77 526 144 230 86 98.69 0.78 527 146 222 76 99.88 0.95 528 146 223 77 99.81 0.97 529 146 226 80 99.78 0.97 530 146 227 81 99.72 0.98 531 147 223 76 99.84 0.96 532 149 176 27 571.52 0.10 533 150 176 26 571.60 0.09 534 151 176 25 571.60 0.09 535 152 174 22 571.89 0.10 536 152 175 23 571.76 0.07 537 152 176 24 571.63 0.10 538 153 175 22 571.86 0.09 539 153 177 24 571.61 0.09 540 153 179 26 571.60 0.09 541 153 182 29 554.17 1.76 542 153 200 47 497.86 1.38 543 154 175 21 572.00 0.10 544 154 177 23 571.84 0.05 545 154 178 24 571.61 0.09 546 154 179 25 571.61 0.09 547 155 179 24 571.61 0.09 548 155 180 25 571.58 0.10 549 155 181 26 571.56 0.10 550 155 182 27 570.76 0.10 551 155 183 28 554.49 1.60 552 156 159 3 841.57 1.49 553 156 162 6 572.86 0.22 554 156 178 22 572.00 0.10 555 156 179 23 571.87 0.07 556 156 180 24 571.60 0.09 557 157 180 23 571.87 0.07 558 158 161 3 798.66 2.66 559 158 162 4 783.91 1.80 560 159 180 21 572.00 0.09 561 161 166 5 788.49 4.11 562 162 166 4 788.37 4.05 563 162 167 5 782.50 1.12 564 162 178 16 572.18 0.14 565 163 166 3 788.63 4.04 566 163 167 4 788.37 4.05 567 164 167 3 797.40 2.79 568 164 168 4 792.03 5.48 569 164 206 42 455.54 0.26 570 164 207 43 437.28 1.09 571 165 168 3 809.16 3.08 572 165 169 4 791.63 5.68 573 166 169 3 799.81 3.85 574 166 170 4 791.21 2.61 575 167 170 3 798.21 0.89 576 167 171 4 791.95 2.23 577 167 178 11 572.20 0.14 578 167 206 39 455.94 1.53 579 168 171 3 791.94 2.23 580 168 172 4 791.95 2.22 581 169 172 3 801.49 0.17 582 169 173 4 800.25 0.78 583 170 173 3 800.25 0.78 584 170 174 4 789.03 0.77 585 170 175 5 753.96 1.40 586 170 178 8 572.54 0.01 587 171 174 3 785.45 2.56 588 171 175 4 763.40 6.12 589 175 178 3 901.13 0.97 590 178 206 28 423.76 0.12 591 179 182 3 865.53 0.70 592 179 200 21 496.94 1.21 593 181 184 3 823.98 0.66 594 181 185 4 802.19 0.78 595 182 185 3 802.59 0.58 596 182 186 4 802.59 0.58 597 182 198 16 497.66 1.48 598 183 186 3 803.44 1.00 599 183 187 4 795.61 2.41 600 184 187 3 806.75 2.30 601 184 188 4 786.38 2.21 602 185 188 3 803.27 3.16 603 185 189 4 785.51 2.65 604 185 196 11 498.26 1.29 605 185 197 12 498.24 1.30 606 186 189 3 800.21 4.70 607 186 190 4 800.20 4.70 608 186 196 10 498.32 1.27 609 186 197 11 498.24 1.30 610 186 198 12 498.22 1.31 611 187 190 3 805.12 2.23 612 187 191 4 795.30 7.14 613 188 191 3 784.72 1.98 614 188 192 4 780.87 0.04 615 189 192 3 804.85 2.36 616 189 193 4 768.31 4.21 617 189 194 5 536.82 17.72 618 189 196 7 498.48 1.23 619 190 193 3 780.50 0.15 620 195 235 40 62.79 1.54 621 199 224 25 98.83 0.80 622 199 227 28 94.68 0.14 623 199 228 29 92.09 1.03 624 200 224 24 98.84 0.79 625 201 217 16 99.83 0.96 626 201 220 19 99.56 0.97 627 201 221 20 99.41 0.99 628 201 224 23 99.34 0.95 629 202 217 15 99.87 0.97 630 203 211 8 100.15 0.94 631 203 212 9 100.12 0.93 632 203 217 14 99.84 0.97 633 204 208 4 771.72 4.07 634 204 211 7 100.16 0.94 635 205 208 3 828.73 3.60 636 211 217 6 756.24 8.58 637 212 217 5 765.53 3.94 638 213 216 3 810.37 1.17 639 213 217 4 787.25 6.38 640 214 217 3 802.20 1.10 641 214 218 4 784.61 5.05 642 215 218 3 801.40 0.57 643 215 219 4 788.27 3.23 644 216 219 3 802.71 1.23 645 216 220 4 801.39 0.57 646 217 220 3 791.05 4.62 647 217 221 4 784.64 5.03 648 218 221 3 797.78 1.03 649 218 222 4 784.63 5.04 650 219 222 3 778.15 1.96 651 219 223 4 774.09 0.24 652 220 223 3 789.73 5.15 653 220 224 4 774.76 0.51 654 221 224 3 788.73 4.16 655 221 225 4 775.03 0.65 656 222 225 3 810.69 6.83 657 222 226 4 778.54 1.81 658 223 226 3 807.26 8.54 659 223 227 4 778.56 1.80 660 224 227 3 791.07 6.30 661 224 228 4 778.57 1.80 662 225 228 3 795.51 4.08 663 225 229 4 778.56 1.80 664 226 229 3 795.98 0.98 665 226 230 4 777.96 2.09 666 227 230 3 800.51 2.38 667 227 231 4 781.53 0.06 668 228 231 3 795.21 3.95 669 228 232 4 782.56 0.58 670 229 232 3 800.74 1.18 671 229 233 4 798.35 0.50 672 230 233 3 798.51 0.42 673 230 234 4 798.35 0.50 674 231 234 3 799.54 0.10 675 231 235 4 788.07 3.26 676 232 235 3 807.64 2.58