BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 9 7 26.72 0.68 2 3 6 3 814.96 3.18 3 3 7 4 676.84 42.18 4 3 8 5 442.78 74.84 5 3 76 73 28.92 0.48 6 3 80 77 28.91 0.48 7 4 7 3 819.26 1.68 8 4 43 39 49.58 0.03 9 4 76 72 39.70 1.70 10 5 43 38 49.48 0.01 11 5 44 39 49.48 0.01 12 5 46 41 48.84 0.20 13 5 72 67 45.56 1.03 14 5 76 71 42.33 0.58 15 6 40 34 74.91 12.70 16 6 41 35 49.47 0.01 17 6 46 40 49.30 0.01 18 6 61 55 46.62 1.22 19 6 73 67 45.57 1.03 20 6 76 70 45.56 1.03 21 6 80 74 137.35 8.18 22 7 41 34 74.96 12.67 23 7 43 36 63.34 0.19 24 8 12 4 756.49 3.74 25 8 40 32 105.36 1.50 26 8 80 72 157.93 0.13 27 9 12 3 790.12 1.08 28 9 80 71 157.93 0.13 29 10 13 3 829.34 0.52 30 10 80 70 157.93 0.13 31 12 40 28 231.86 1.03 32 12 50 38 239.53 4.64 33 13 17 4 801.85 1.30 34 13 40 27 239.52 4.65 35 13 50 37 252.44 1.78 36 13 59 46 240.00 4.42 37 13 77 64 206.79 0.43 38 13 80 67 211.68 0.15 39 13 82 69 211.57 0.19 40 14 17 3 831.38 1.49 41 14 50 36 262.36 0.56 42 14 57 43 292.13 0.32 43 14 58 44 290.61 0.80 44 14 59 45 255.81 0.14 45 14 82 68 211.89 0.14 46 15 81 66 212.01 0.12 47 15 82 67 211.91 0.13 48 15 83 68 211.83 0.12 49 15 84 69 211.81 0.12 50 16 56 40 292.63 0.26 51 16 58 42 292.22 0.34 52 16 59 43 292.22 0.34 53 16 83 67 211.94 0.14 54 16 84 68 211.78 0.10 55 16 232 216 69.27 0.29 56 16 233 217 69.27 0.29 57 16 235 219 53.66 0.32 58 17 59 42 292.23 0.34 59 17 60 43 292.19 0.33 60 17 61 44 292.19 0.33 61 17 77 60 212.41 0.11 62 17 82 65 212.27 0.11 63 17 84 67 211.94 0.14 64 17 85 68 211.84 0.13 65 17 86 69 211.81 0.12 66 18 29 11 292.60 0.28 67 18 58 40 292.65 0.26 68 18 59 41 292.33 0.29 69 18 60 42 292.21 0.33 70 18 61 43 292.20 0.33 71 18 86 68 211.88 0.15 72 18 88 70 211.69 0.08 73 18 225 207 94.76 0.02 74 18 228 210 92.09 1.03 75 18 229 211 92.06 1.03 76 18 232 214 69.29 0.28 77 19 61 42 292.22 0.34 78 19 63 44 292.08 0.37 79 19 74 55 214.25 0.01 80 19 77 58 214.56 0.18 81 19 85 66 212.19 0.07 82 19 86 67 211.94 0.14 83 19 87 68 211.84 0.13 84 19 88 69 211.84 0.14 85 19 185 166 212.11 0.10 86 20 25 5 754.79 3.47 87 20 26 6 349.46 2.29 88 20 29 9 708.04 6.55 89 20 60 40 292.64 0.26 90 20 61 41 292.41 0.27 91 20 62 42 292.21 0.32 92 20 63 43 292.20 0.33 93 20 88 68 211.91 0.14 94 21 25 4 807.26 4.62 95 21 62 41 292.34 0.30 96 21 63 42 292.23 0.32 97 21 64 43 292.14 0.33 98 22 25 3 811.52 2.48 99 22 26 4 788.84 3.97 100 22 62 40 292.62 0.26 101 23 26 3 800.81 2.02 102 23 27 4 800.81 2.02 103 23 45 22 292.50 0.28 104 23 47 24 292.53 0.30 105 23 62 39 292.56 0.23 106 24 27 3 801.98 1.48 107 24 28 4 788.84 3.97 108 25 28 3 798.34 3.32 109 25 29 4 772.48 4.18 110 25 88 63 212.12 0.05 111 25 89 64 211.91 0.14 112 25 90 65 211.91 0.14 113 25 221 196 95.28 0.01 114 25 224 199 95.22 0.04 115 26 29 3 799.12 3.71 116 26 30 4 783.41 1.31 117 26 47 21 292.70 0.28 118 26 49 23 292.85 0.27 119 26 60 34 292.81 0.29 120 26 62 36 292.50 0.20 121 27 30 3 786.57 3.31 122 27 31 4 781.60 4.80 123 27 47 20 292.75 0.29 124 27 49 22 292.87 0.25 125 28 31 3 805.76 1.07 126 28 32 4 771.13 0.42 127 28 33 5 781.59 4.80 128 28 218 190 95.44 0.02 129 28 221 193 95.36 0.02 130 28 222 194 95.27 0.01 131 28 225 197 94.98 0.06 132 29 32 3 787.34 5.33 133 29 33 4 771.09 0.45 134 29 34 5 763.42 0.14 135 29 60 31 292.86 0.31 136 29 88 59 211.90 0.14 137 29 225 196 94.91 0.06 138 30 34 4 760.05 1.43 139 30 37 7 292.83 0.22 140 30 49 19 292.90 0.28 141 33 53 20 341.49 0.61 142 33 222 189 94.98 0.05 143 33 225 192 94.95 0.04 144 33 226 193 94.94 0.04 145 33 229 196 92.16 1.02 146 34 37 3 804.00 5.78 147 34 51 17 340.25 0.02 148 34 52 18 341.38 0.56 149 34 53 19 341.50 0.61 150 34 58 24 339.64 0.92 151 34 60 26 325.42 0.38 152 34 229 195 92.14 1.02 153 35 51 16 342.86 0.02 154 35 52 17 342.54 0.10 155 36 51 15 342.91 0.00 156 36 52 16 342.92 0.01 157 37 50 13 342.92 0.01 158 37 51 14 342.91 0.00 159 38 49 11 343.01 0.01 160 38 50 12 342.95 0.01 161 39 42 3 886.95 0.99 162 39 47 8 343.23 0.04 163 39 48 9 343.13 0.07 164 39 49 10 343.03 0.02 165 40 48 8 343.23 0.03 166 40 49 9 343.15 0.05 167 40 50 10 343.14 0.05 168 40 61 21 342.87 0.01 169 40 77 37 213.80 0.61 170 41 46 5 522.98 85.30 171 41 47 6 343.26 0.01 172 41 48 7 343.23 0.03 173 42 45 3 781.93 4.76 174 42 47 5 343.33 0.01 175 43 46 3 806.75 1.72 176 46 61 15 343.00 0.03 177 46 62 16 342.81 0.04 178 46 63 17 342.79 0.03 179 46 64 18 342.75 0.01 180 46 73 27 215.09 0.09 181 47 61 14 342.95 0.01 182 47 62 15 342.79 0.03 183 48 60 12 342.98 0.03 184 48 61 13 342.70 0.01 185 49 59 10 342.99 0.04 186 49 60 11 342.88 0.06 187 50 58 8 343.29 0.14 188 50 59 9 342.91 0.09 189 51 57 6 343.48 0.18 190 51 58 7 343.37 0.16 191 52 55 3 795.09 7.19 192 52 56 4 529.33 93.06 193 52 57 5 343.50 0.18 194 53 56 3 784.02 1.66 195 53 58 5 523.75 90.30 196 53 229 176 92.13 1.02 197 53 230 177 92.11 1.03 198 53 233 180 69.27 0.28 199 54 230 176 92.14 1.02 200 54 233 179 69.30 0.28 201 56 233 177 69.14 0.21 202 58 229 171 92.01 1.05 203 58 233 175 69.26 0.28 204 61 73 12 214.65 0.21 205 61 77 16 214.25 0.24 206 62 73 11 214.76 0.24 207 63 69 6 401.38 93.04 208 63 70 7 214.60 0.31 209 63 73 10 214.76 0.24 210 64 69 5 705.81 10.04 211 64 181 117 214.59 0.17 212 65 69 4 793.12 1.28 213 65 181 116 214.63 0.18 214 66 69 3 818.25 0.16 215 66 70 4 788.86 0.61 216 66 180 114 214.38 0.07 217 66 181 115 214.72 0.17 218 67 70 3 797.48 0.90 219 67 71 4 779.89 2.01 220 67 113 46 215.13 0.04 221 67 115 48 215.27 0.07 222 67 155 88 215.02 0.05 223 67 180 113 214.86 0.10 224 67 181 114 214.88 0.11 225 67 183 116 215.06 0.07 226 67 184 117 215.06 0.07 227 68 71 3 795.75 0.03 228 68 72 4 788.86 0.60 229 68 115 47 215.39 0.02 230 69 72 3 805.76 5.05 231 69 73 4 795.61 5.30 232 70 73 3 799.62 3.30 233 70 74 4 784.25 3.29 234 70 181 111 215.09 0.04 235 71 74 3 800.56 2.83 236 71 75 4 788.00 1.41 237 71 115 44 215.50 0.03 238 71 119 48 216.12 0.21 239 71 184 113 215.41 0.06 240 71 188 117 215.86 0.18 241 72 75 3 807.31 4.34 242 72 76 4 791.92 0.55 243 73 76 3 800.56 1.54 244 73 77 4 792.83 3.77 245 74 77 3 794.75 4.73 246 74 78 4 793.99 4.34 247 74 85 11 215.52 0.04 248 74 185 111 215.79 0.18 249 74 188 114 216.33 0.07 250 75 78 3 804.40 0.10 251 75 79 4 794.75 4.72 252 75 119 44 216.74 0.03 253 75 188 113 216.38 0.14 254 75 192 117 217.19 0.01 255 76 79 3 795.09 4.55 256 76 80 4 782.99 3.48 257 77 80 3 798.75 1.96 258 77 81 4 782.99 3.49 259 77 82 5 730.58 8.86 260 77 85 8 216.43 0.12 261 78 81 3 795.68 5.63 262 78 82 4 759.50 2.98 263 78 192 114 338.24 13.84 264 78 194 116 357.33 4.45 265 82 85 3 851.46 2.10 266 82 194 112 494.41 2.07 267 83 194 111 494.40 2.08 268 83 195 112 494.40 2.08 269 83 235 152 60.36 1.78 270 84 133 49 494.39 2.08 271 84 195 111 494.40 2.08 272 84 232 148 69.28 0.29 273 84 235 151 53.62 0.34 274 85 185 100 494.44 2.07 275 85 194 109 494.51 2.06 276 85 195 110 494.44 2.07 277 85 196 111 494.41 2.08 278 85 197 112 494.40 2.08 279 86 133 47 494.44 2.07 280 86 137 51 494.41 2.08 281 86 197 111 494.41 2.08 282 86 198 112 494.40 2.08 283 86 199 113 494.38 2.08 284 86 228 142 92.09 1.03 285 86 232 146 69.28 0.29 286 87 179 92 494.43 2.08 287 87 181 94 494.45 2.08 288 87 182 95 494.44 2.07 289 87 185 98 494.57 2.04 290 87 197 110 494.45 2.06 291 87 198 111 494.41 2.08 292 87 199 112 494.41 2.08 293 87 200 113 494.40 2.08 294 88 199 111 494.42 2.07 295 88 224 136 94.97 0.05 296 88 225 137 94.86 0.04 297 88 228 140 92.09 1.03 298 89 199 110 494.46 2.06 299 89 200 111 494.41 2.08 300 89 201 112 494.40 2.07 301 89 224 135 95.19 0.03 302 90 179 89 494.54 2.06 303 90 200 110 494.44 2.07 304 90 201 111 494.41 2.08 305 90 203 113 490.70 0.77 306 91 177 86 494.66 2.01 307 91 178 87 494.62 2.00 308 91 179 88 494.54 2.06 309 91 200 109 494.50 2.06 310 91 201 110 494.46 2.06 311 91 202 111 494.37 2.08 312 91 203 112 494.29 2.09 313 91 204 113 493.19 1.68 314 92 177 85 494.67 2.01 315 92 178 86 494.67 2.01 316 92 204 112 493.25 1.72 317 92 206 114 438.98 1.50 318 93 98 5 494.43 2.08 319 93 176 83 494.68 2.00 320 93 177 84 494.67 2.00 321 93 202 109 494.50 2.06 322 93 204 111 494.39 2.07 323 93 205 112 452.57 0.29 324 93 206 113 443.80 1.02 325 94 167 73 494.64 1.99 326 94 168 74 494.65 1.99 327 94 171 77 494.63 1.99 328 94 175 81 494.68 2.00 329 94 176 82 494.68 2.01 330 94 177 83 494.72 2.00 331 94 178 84 494.73 2.00 332 95 98 3 818.08 2.00 333 95 168 73 494.69 2.00 334 95 171 76 494.70 2.00 335 96 168 72 494.75 2.00 336 96 205 109 494.47 2.06 337 97 149 52 497.92 3.58 338 98 102 4 782.12 2.09 339 98 104 6 710.35 10.46 340 98 149 51 497.87 3.60 341 98 176 78 494.85 1.99 342 98 177 79 494.90 2.00 343 98 202 104 494.75 2.02 344 98 205 107 494.49 2.07 345 99 102 3 815.66 0.89 346 99 202 103 494.78 2.02 347 99 205 106 494.60 2.04 348 100 202 102 494.77 2.02 349 100 204 104 494.68 2.03 350 100 205 105 494.68 2.03 351 100 212 112 97.11 0.01 352 100 216 116 96.44 0.20 353 101 202 101 494.81 2.01 354 101 220 119 96.12 0.14 355 102 149 47 685.04 1.55 356 102 201 99 494.94 2.02 357 102 202 100 494.88 2.03 358 103 146 43 685.85 1.90 359 103 147 44 685.76 1.85 360 103 148 45 685.70 1.87 361 103 149 46 685.70 1.87 362 103 200 97 495.12 2.02 363 103 201 98 494.94 2.02 364 103 220 117 96.09 0.14 365 103 223 120 96.06 0.16 366 103 224 121 95.41 0.04 367 104 149 45 685.70 1.87 368 104 153 49 623.46 1.61 369 104 176 72 560.34 1.36 370 104 177 73 548.43 0.85 371 104 199 95 495.27 2.05 372 104 200 96 495.16 2.01 373 104 202 98 495.12 2.02 374 105 134 29 686.21 1.79 375 105 137 32 686.19 1.80 376 105 138 33 686.11 1.83 377 105 149 44 685.91 1.82 378 105 150 45 685.83 1.81 379 105 151 46 685.83 1.81 380 105 198 93 495.51 2.02 381 105 199 94 495.34 2.04 382 106 129 23 687.13 1.54 383 106 134 28 686.32 1.80 384 106 151 45 685.84 1.80 385 106 153 47 675.91 1.92 386 106 186 80 547.17 0.59 387 106 198 92 495.52 2.02 388 106 200 94 495.44 2.02 389 107 128 21 687.19 1.54 390 107 129 22 687.14 1.53 391 107 134 27 686.33 1.81 392 107 150 43 686.00 1.79 393 107 151 44 685.87 1.80 394 108 127 19 687.22 1.54 395 108 128 20 687.19 1.54 396 108 129 21 687.20 1.54 397 108 152 44 685.88 1.80 398 108 153 45 685.87 1.80 399 108 186 78 547.60 0.55 400 109 127 18 687.24 1.54 401 109 152 43 686.00 1.79 402 109 153 44 685.87 1.80 403 109 155 46 685.83 1.81 404 109 182 73 553.78 1.57 405 109 183 74 552.16 1.69 406 109 186 77 547.65 0.54 407 110 153 43 686.00 1.79 408 110 154 44 685.87 1.80 409 110 155 45 685.85 1.81 410 110 170 60 569.22 1.31 411 110 175 65 569.20 1.32 412 111 116 5 724.78 0.92 413 111 125 14 687.38 1.55 414 111 127 16 687.33 1.52 415 111 183 72 553.51 1.40 416 112 154 42 686.07 1.79 417 112 155 43 685.94 1.81 418 112 156 44 685.86 1.81 419 112 170 58 569.29 1.32 420 112 178 66 569.16 1.32 421 113 116 3 823.08 1.06 422 113 117 4 792.17 4.76 423 113 155 42 686.06 1.80 424 113 157 44 685.86 1.81 425 113 183 70 566.47 2.19 426 114 117 3 804.16 2.89 427 114 118 4 792.20 4.78 428 114 157 43 685.97 1.76 429 115 118 3 813.03 1.54 430 115 119 4 792.19 4.78 431 115 184 69 565.20 2.83 432 116 119 3 792.23 4.76 433 116 120 4 782.08 9.84 434 116 183 67 565.23 2.84 435 116 184 68 564.73 3.02 436 116 187 71 554.19 1.36 437 117 120 3 792.21 4.78 438 117 121 4 760.62 0.67 439 117 122 5 718.44 0.03 440 118 121 3 789.51 6.12 441 119 187 68 554.35 1.38 442 119 188 69 554.28 1.38 443 119 191 72 548.61 0.77 444 119 192 73 548.68 0.74 445 120 123 3 783.13 2.94 446 120 125 5 717.46 6.54 447 120 187 67 554.31 1.38 448 125 187 62 551.19 1.22 449 125 190 65 547.86 0.51 450 127 183 56 551.33 1.26 451 127 186 59 547.65 0.55 452 128 186 58 547.52 0.54 453 128 190 62 539.97 0.45 454 129 134 5 763.55 6.57 455 129 186 57 547.25 0.64 456 129 190 61 539.95 0.46 457 129 196 67 496.45 2.17 458 129 197 68 495.86 2.00 459 130 133 3 794.65 0.73 460 130 134 4 782.24 6.95 461 130 190 60 539.97 0.45 462 130 195 65 496.60 2.15 463 130 196 66 496.60 2.14 464 131 134 3 798.20 1.02 465 131 135 4 785.25 5.45 466 131 190 59 539.99 0.44 467 132 135 3 804.57 1.53 468 132 136 4 785.25 5.45 469 132 195 63 496.78 2.21 470 132 235 103 69.30 0.28 471 133 136 3 795.47 0.36 472 133 137 4 785.25 5.45 473 133 195 62 496.76 2.21 474 133 196 63 496.73 2.21 475 133 197 64 496.50 2.19 476 133 235 102 69.11 0.36 477 134 137 3 803.46 3.62 478 134 138 4 785.28 5.46 479 134 150 16 686.10 1.83 480 135 138 3 794.19 1.01 481 135 139 4 783.31 6.42 482 136 139 3 794.65 1.24 483 136 140 4 774.03 1.76 484 136 231 95 82.94 1.47 485 136 234 98 70.22 0.22 486 136 235 99 69.48 0.36 487 137 140 3 807.15 4.23 488 137 141 4 779.93 1.20 489 137 197 60 496.64 2.27 490 137 198 61 496.56 2.23 491 137 227 90 93.73 0.54 492 137 228 91 92.14 1.03 493 137 231 94 80.48 0.25 494 138 141 3 807.15 4.23 495 138 142 4 787.81 1.40 496 138 148 10 686.39 1.74 497 138 150 12 686.08 1.80 498 139 142 3 787.10 4.06 499 139 143 4 773.97 1.74 500 140 143 3 785.39 0.20 501 140 144 4 773.30 1.88 502 140 227 87 94.69 0.12 503 140 230 90 92.09 1.03 504 140 231 91 92.09 1.03 505 140 234 94 91.56 1.27 506 141 144 3 801.09 3.14 507 141 145 4 773.64 1.71 508 141 146 5 732.54 6.07 509 141 148 7 687.51 1.25 510 141 227 86 94.91 0.07 511 142 145 3 792.21 7.58 512 142 148 6 695.23 0.87 513 144 226 82 96.05 0.20 514 144 227 83 95.75 0.21 515 144 230 86 95.23 0.01 516 146 222 76 96.39 0.23 517 146 223 77 96.25 0.21 518 146 226 80 96.22 0.21 519 146 227 81 96.22 0.21 520 147 223 76 96.32 0.19 521 149 176 27 568.57 1.41 522 150 176 26 568.65 1.38 523 151 176 25 568.66 1.38 524 152 174 22 569.04 1.32 525 152 175 23 568.79 1.31 526 152 176 24 568.79 1.31 527 153 175 22 568.99 1.35 528 153 177 24 568.66 1.38 529 153 179 26 568.64 1.39 530 153 182 29 554.32 1.66 531 153 200 47 495.20 2.06 532 154 175 21 569.16 1.31 533 154 177 23 568.99 1.38 534 154 178 24 568.67 1.38 535 154 179 25 568.66 1.38 536 155 179 24 568.69 1.37 537 155 180 25 568.62 1.38 538 155 182 27 564.99 2.71 539 155 183 28 554.38 1.66 540 156 159 3 863.28 1.43 541 156 162 6 569.70 1.33 542 156 178 22 569.14 1.33 543 156 179 23 568.99 1.38 544 156 180 24 568.66 1.38 545 157 180 23 568.87 1.41 546 158 161 3 798.66 2.66 547 159 180 21 569.18 1.32 548 162 166 4 782.19 3.11 549 162 167 5 781.60 0.92 550 162 206 44 479.23 0.22 551 163 166 3 788.05 2.86 552 163 167 4 785.40 1.53 553 163 206 43 487.47 0.90 554 164 167 3 797.40 2.79 555 164 168 4 788.44 0.02 556 164 206 42 475.70 1.99 557 164 207 43 475.32 2.17 558 165 168 3 809.16 3.08 559 165 169 4 795.70 3.65 560 166 169 3 797.55 2.73 561 166 170 4 788.59 0.04 562 167 170 3 798.16 0.91 563 167 171 4 788.62 0.06 564 167 178 11 569.28 1.33 565 167 206 39 467.32 2.20 566 168 171 3 798.16 0.91 567 168 172 4 798.17 0.91 568 169 172 3 823.40 0.63 569 169 173 4 802.00 3.62 570 170 173 3 806.48 2.50 571 170 174 4 798.85 5.18 572 170 175 5 743.59 6.28 573 170 178 8 569.86 1.36 574 171 174 3 788.61 0.07 575 171 175 4 765.89 4.88 576 175 178 3 901.13 0.97 577 178 206 28 440.56 0.72 578 179 182 3 865.53 0.70 579 179 200 21 494.94 2.21 580 181 184 3 823.98 0.66 581 181 185 4 797.28 3.23 582 182 185 3 802.59 0.58 583 182 186 4 785.53 2.65 584 182 198 16 495.31 2.02 585 183 186 3 802.59 0.58 586 183 187 4 795.61 2.40 587 184 187 3 800.92 0.26 588 184 188 4 785.81 2.50 589 185 188 3 805.51 2.04 590 185 189 4 785.83 2.48 591 185 196 11 495.97 1.84 592 185 197 12 495.88 1.88 593 186 189 3 799.69 4.95 594 186 190 4 799.68 4.96 595 186 196 10 495.88 1.86 596 186 197 11 495.86 1.86 597 186 198 12 495.81 1.85 598 187 190 3 799.69 4.96 599 187 191 4 785.32 2.23 600 188 191 3 781.55 0.35 601 188 192 4 771.23 4.87 602 189 193 4 740.83 4.24 603 189 194 5 496.58 2.12 604 189 196 7 496.50 2.10 605 190 193 3 763.78 2.62 606 199 224 25 95.08 0.08 607 199 227 28 93.45 0.40 608 199 228 29 92.09 1.03 609 200 224 24 95.20 0.03 610 201 217 16 96.22 0.19 611 201 220 19 95.83 0.11 612 201 221 20 95.31 0.01 613 201 224 23 95.35 0.03 614 202 217 15 96.24 0.18 615 203 211 8 97.09 0.01 616 203 212 9 96.91 0.03 617 203 217 14 96.20 0.18 618 204 208 4 738.66 12.46 619 204 211 7 97.12 0.01 620 205 208 3 806.66 2.10 621 213 216 3 811.90 0.41 622 213 217 4 787.25 6.38 623 214 217 3 805.36 2.68 624 214 218 4 773.94 0.28 625 215 218 3 801.26 0.64 626 215 219 4 773.94 0.28 627 217 220 3 780.57 0.41 628 217 221 4 777.19 2.09 629 218 221 3 796.67 1.59 630 218 222 4 777.21 2.08 631 219 222 3 786.10 2.00 632 219 223 4 777.27 2.04 633 220 223 3 790.69 4.67 634 220 224 4 777.26 2.03 635 221 224 3 781.33 0.00 636 221 225 4 778.14 1.57 637 222 225 3 802.84 10.76 638 222 226 4 778.14 1.58 639 223 226 3 787.00 3.08 640 223 227 4 778.33 1.68 641 224 227 3 792.26 5.70 642 224 228 4 778.34 1.68 643 225 228 3 795.51 4.08 644 225 229 4 778.32 1.69 645 226 229 3 799.25 0.65 646 226 230 4 784.49 1.44 647 227 230 3 800.51 2.38 648 227 231 4 781.51 0.04 649 228 231 3 788.68 2.50 650 228 232 4 781.97 0.41 651 229 232 3 799.95 0.99 652 229 233 4 786.62 1.49 653 230 233 3 798.51 0.42 654 230 234 4 790.50 3.43 655 231 234 3 796.96 1.19 656 231 235 4 788.29 3.15 657 232 235 3 807.64 2.58