BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 247 244 27.80 0.34 2 3 248 245 19.67 0.09 3 3 249 246 17.27 0.51 4 4 248 244 25.91 0.93 5 5 116 111 42.34 0.07 6 5 240 235 42.94 0.23 7 5 244 239 39.46 0.61 8 5 247 242 29.15 0.60 9 5 248 243 27.12 0.89 10 6 117 111 43.11 0.20 11 6 118 112 39.62 0.93 12 6 247 241 33.20 1.19 13 6 248 242 28.66 0.55 14 8 12 4 858.78 6.42 15 8 117 109 44.79 0.20 16 8 118 110 44.57 0.09 17 9 12 3 865.09 3.31 18 9 13 4 865.06 3.27 19 10 13 3 881.67 0.70 20 10 14 4 865.08 3.26 21 11 14 3 865.89 3.64 22 11 15 4 855.18 1.72 23 11 90 79 519.30 0.94 24 12 15 3 874.66 2.77 25 12 16 4 868.88 5.13 26 12 90 78 519.36 0.92 27 12 91 79 519.29 0.95 28 12 92 80 519.36 0.92 29 12 95 83 519.41 0.94 30 12 117 105 519.40 0.95 31 13 16 3 870.62 4.26 32 13 17 4 865.89 3.64 33 13 117 104 519.40 0.95 34 14 17 3 867.84 2.87 35 14 18 4 856.11 2.19 36 15 18 3 870.50 1.53 37 15 19 4 853.11 3.69 38 15 88 73 523.34 1.57 39 15 90 75 523.35 1.57 40 15 91 76 523.29 1.58 41 16 19 3 869.85 1.21 42 16 20 4 853.12 3.69 43 16 91 75 523.41 1.54 44 16 99 83 524.54 2.10 45 16 115 99 538.51 1.02 46 16 117 101 523.39 1.55 47 17 20 3 881.74 7.14 48 17 21 4 859.16 4.15 49 18 21 3 881.74 7.14 50 18 22 4 854.64 2.91 51 18 88 70 541.29 0.46 52 19 22 3 876.02 4.33 53 19 23 4 861.96 0.76 54 19 88 69 541.29 0.46 55 19 91 72 541.26 0.45 56 19 96 77 541.25 0.46 57 19 99 80 541.27 0.45 58 20 23 3 861.98 0.76 59 20 24 4 861.97 0.76 60 20 99 79 541.27 0.46 61 20 103 83 550.48 1.95 62 20 106 86 556.23 0.46 63 21 24 3 864.19 1.87 64 21 25 4 861.97 0.76 65 22 25 3 870.38 4.97 66 22 26 4 865.50 2.54 67 22 84 62 555.21 0.24 68 22 88 66 555.21 0.24 69 23 26 3 867.75 4.40 70 23 27 4 860.38 0.92 71 23 81 58 555.49 0.36 72 23 84 61 555.25 0.25 73 23 96 73 555.22 0.24 74 23 99 76 555.22 0.24 75 23 100 77 555.22 0.24 76 23 103 80 557.18 1.03 77 24 27 3 874.97 2.66 78 24 28 4 860.58 0.82 79 24 103 79 557.28 0.97 80 24 106 82 560.75 0.40 81 24 107 83 560.75 0.40 82 25 28 3 867.79 4.37 83 25 29 4 856.18 0.21 84 26 29 3 863.87 3.64 85 26 30 4 853.00 1.40 86 26 32 6 832.16 3.41 87 26 80 54 582.46 13.51 88 26 81 55 557.69 1.12 89 26 84 58 557.69 1.12 90 27 30 3 865.27 2.93 91 27 31 4 842.68 6.55 92 27 32 5 816.03 0.95 93 27 33 6 802.12 6.34 94 27 36 9 735.06 24.41 95 27 100 73 560.79 0.41 96 27 103 76 561.22 0.55 97 27 104 77 564.90 0.08 98 27 107 80 566.87 0.90 99 28 31 3 876.12 0.64 100 30 80 50 607.41 1.07 101 31 34 3 1027.51 5.55 102 31 184 153 309.94 6.98 103 32 35 3 875.66 0.78 104 32 36 4 845.11 0.03 105 32 80 48 609.91 0.66 106 33 36 3 871.73 4.29 107 33 37 4 849.83 2.35 108 33 104 71 603.53 0.34 109 33 107 74 603.93 0.24 110 33 108 75 603.88 0.21 111 33 165 132 311.11 6.80 112 34 37 3 861.16 0.51 113 34 38 4 854.49 3.84 114 34 165 131 311.11 6.80 115 34 166 132 310.99 6.82 116 34 183 149 310.42 6.94 117 34 184 150 310.32 6.94 118 35 38 3 863.11 0.46 119 35 39 4 854.50 3.83 120 35 74 39 631.09 1.10 121 35 76 41 631.08 1.11 122 35 77 42 631.00 1.15 123 35 80 45 630.94 1.12 124 35 172 137 312.91 6.68 125 36 39 3 860.60 0.79 126 36 40 4 858.06 2.06 127 36 77 41 630.99 1.15 128 36 80 44 630.94 1.12 129 36 81 45 630.92 1.11 130 36 100 64 606.31 0.31 131 36 101 65 606.31 0.31 132 36 104 68 605.84 0.31 133 37 40 3 868.41 2.19 134 37 41 4 854.56 3.80 135 37 101 64 606.31 0.31 136 37 104 67 605.91 0.28 137 37 105 68 605.99 0.24 138 37 162 125 311.31 6.84 139 37 165 128 311.11 6.80 140 37 235 198 199.03 2.06 141 37 239 202 190.28 2.46 142 38 41 3 860.64 0.76 143 38 42 4 847.12 0.10 144 38 70 32 631.86 1.28 145 38 71 33 631.81 1.30 146 38 72 34 631.81 1.30 147 38 73 35 631.68 1.28 148 38 74 36 631.19 1.15 149 38 172 134 312.94 6.70 150 39 42 3 866.23 3.46 151 39 43 4 854.31 2.49 152 39 60 21 631.35 1.22 153 39 74 35 631.24 1.07 154 39 77 38 631.11 1.11 155 39 78 39 631.10 1.11 156 39 81 42 630.97 1.14 157 39 97 58 608.49 0.82 158 40 43 3 867.78 5.57 159 40 44 4 855.55 0.56 160 40 97 57 608.52 0.82 161 40 98 58 608.35 0.76 162 40 101 61 606.38 0.35 163 41 44 3 859.12 1.22 164 41 45 4 850.01 0.34 165 41 131 90 471.62 2.68 166 41 132 91 471.00 2.37 167 41 145 104 346.82 4.96 168 41 149 108 347.48 4.56 169 41 242 201 181.75 0.69 170 42 46 4 855.56 0.56 171 42 60 18 631.76 1.28 172 42 70 28 631.87 1.28 173 42 71 29 631.84 1.29 174 42 74 32 631.72 1.29 175 42 131 89 471.62 2.68 176 43 46 3 914.63 3.92 177 43 55 12 632.79 1.49 178 43 56 13 632.69 1.53 179 43 60 17 631.73 1.29 180 43 98 55 608.73 0.72 181 44 47 3 901.78 1.56 182 44 55 11 632.78 1.49 183 44 56 12 632.69 1.53 184 44 98 54 608.76 0.74 185 44 242 198 181.75 0.69 186 44 243 199 181.69 0.71 187 44 245 201 181.36 0.83 188 45 131 86 471.66 2.69 189 45 149 104 347.44 4.58 190 45 242 197 182.08 0.85 191 46 54 8 632.91 1.50 192 46 55 9 632.89 1.50 193 46 126 80 544.07 2.19 194 46 130 84 481.13 0.92 195 46 131 85 472.19 2.62 196 47 52 5 669.08 10.62 197 47 53 6 633.24 1.38 198 47 54 7 633.12 1.42 199 47 55 8 632.90 1.50 200 47 148 101 348.07 4.49 201 47 149 102 347.81 4.46 202 47 245 198 181.65 0.74 203 47 246 199 181.43 0.83 204 48 51 3 878.84 4.83 205 48 52 4 720.98 23.48 206 48 53 5 636.50 0.20 207 48 130 82 514.36 6.48 208 48 148 100 348.07 4.49 209 49 52 3 847.77 2.73 210 49 146 97 348.15 4.51 211 49 147 98 348.15 4.51 212 49 148 99 348.14 4.51 213 49 149 100 348.10 4.50 214 49 150 101 348.06 4.49 215 53 130 77 536.97 0.15 216 54 57 3 943.50 0.66 217 54 126 72 538.88 0.14 218 54 130 76 508.22 9.30 219 56 94 38 609.55 0.55 220 56 98 42 608.79 0.74 221 56 119 63 603.72 0.23 222 56 243 187 181.74 0.70 223 56 245 189 181.37 0.83 224 57 94 37 609.58 0.53 225 57 119 62 603.77 0.21 226 57 120 63 603.65 0.25 227 57 121 64 603.25 0.32 228 57 122 65 600.80 0.01 229 57 245 188 181.53 0.78 230 57 246 189 181.35 0.83 231 57 247 190 180.81 0.65 232 58 61 3 901.26 5.11 233 58 93 35 609.63 0.54 234 58 94 36 609.60 0.54 235 58 121 63 603.08 0.30 236 59 62 3 877.02 2.52 237 59 65 6 822.34 0.29 238 59 122 63 596.69 0.71 239 59 124 65 576.61 7.24 240 59 125 66 551.32 1.40 241 59 126 67 551.33 1.39 242 60 65 5 822.36 0.30 243 60 66 6 822.33 0.29 244 60 71 11 636.12 1.22 245 60 74 14 632.31 1.22 246 60 78 18 632.01 1.33 247 60 97 37 608.78 0.73 248 61 66 5 831.83 4.42 249 61 78 17 631.97 1.33 250 61 93 32 609.65 0.55 251 61 97 36 609.09 0.58 252 62 65 3 889.63 1.03 253 62 66 4 870.50 8.54 254 62 122 60 603.14 0.28 255 62 123 61 592.86 1.19 256 62 125 63 557.88 1.62 257 63 66 3 871.50 8.04 258 63 67 4 870.50 8.54 259 64 67 3 889.63 1.02 260 64 68 4 881.68 2.94 261 65 68 3 876.75 0.61 262 65 71 6 737.93 20.58 263 65 125 60 551.79 1.24 264 65 126 61 551.78 1.23 265 66 71 5 690.28 0.40 266 66 74 8 645.31 5.28 267 66 75 9 632.14 1.31 268 66 78 12 632.13 1.31 269 68 71 3 873.96 2.81 270 68 72 4 858.75 0.04 271 68 126 58 552.03 1.35 272 68 127 59 551.45 1.37 273 68 128 60 536.45 0.76 274 69 72 3 868.33 4.83 275 69 128 59 540.09 0.64 276 70 131 61 483.33 0.01 277 71 74 3 865.26 0.77 278 71 131 60 482.42 0.47 279 72 172 100 312.73 6.67 280 73 76 3 880.54 0.47 281 73 172 99 312.73 6.67 282 74 77 3 872.00 4.67 283 74 78 4 850.44 0.03 284 75 78 3 869.06 3.19 285 75 79 4 863.87 3.26 286 76 79 3 871.08 0.34 287 76 80 4 863.94 3.23 288 76 172 96 312.73 6.67 289 77 80 3 883.62 3.35 290 77 81 4 867.14 1.62 291 78 81 3 879.14 5.59 292 78 82 4 869.81 2.96 293 78 97 19 608.76 0.73 294 79 82 3 884.89 2.72 295 79 83 4 893.09 1.38 296 80 83 3 928.26 0.74 297 81 84 3 888.20 3.83 298 81 85 4 868.03 0.07 299 81 96 15 608.84 0.77 300 81 97 16 608.76 0.73 301 81 100 19 607.97 1.04 302 82 85 3 872.19 1.60 303 82 86 4 868.06 0.06 304 82 93 11 622.40 5.85 305 83 86 3 872.15 1.62 306 83 87 4 868.54 0.18 307 84 87 3 872.45 1.77 308 84 88 4 868.54 0.18 309 84 96 12 622.42 5.84 310 85 88 3 874.15 0.58 311 85 89 4 867.18 2.30 312 85 91 6 834.28 2.35 313 85 92 7 817.43 6.25 314 85 93 8 641.75 0.08 315 85 96 11 621.19 5.19 316 86 89 3 886.93 2.47 317 88 91 3 875.06 0.40 318 88 96 8 711.41 1.04 319 91 96 5 837.58 1.16 320 92 95 3 867.10 0.66 321 92 96 4 845.41 2.75 322 92 119 27 604.00 0.14 323 92 120 28 603.85 0.17 324 92 121 29 603.61 0.26 325 93 96 3 873.71 2.75 326 93 97 4 862.79 2.02 327 93 121 28 603.34 0.27 328 94 97 3 880.39 0.59 329 94 98 4 863.00 1.94 330 94 119 25 603.91 0.14 331 94 120 26 603.68 0.25 332 94 121 27 603.08 0.29 333 95 98 3 871.21 1.49 334 95 99 4 863.00 1.93 335 95 115 20 604.39 0.18 336 95 117 22 604.09 0.14 337 95 118 23 604.05 0.12 338 95 119 24 603.92 0.14 339 96 99 3 863.96 2.41 340 96 100 4 862.99 1.93 341 97 100 3 862.97 1.92 342 97 101 4 862.96 1.92 343 98 101 3 871.04 2.15 344 98 102 4 862.92 1.91 345 98 115 17 604.35 0.19 346 98 119 21 603.91 0.14 347 98 243 145 181.43 0.80 348 99 102 3 862.88 1.90 349 99 103 4 862.87 1.90 350 99 115 16 604.35 0.19 351 100 103 3 859.77 0.35 352 100 104 4 856.51 1.28 353 101 104 3 860.03 0.48 354 101 105 4 863.63 2.29 355 101 239 138 190.28 2.46 356 102 105 3 880.33 7.74 357 102 106 4 863.61 2.30 358 102 113 11 604.39 0.17 359 102 239 137 190.28 2.46 360 103 106 3 860.61 3.79 361 103 107 4 860.60 3.80 362 104 107 3 887.34 6.57 363 104 108 4 856.56 1.88 364 105 108 3 876.70 1.25 365 105 109 4 856.53 1.90 366 105 110 5 777.09 0.65 367 105 111 6 777.08 0.65 368 105 113 8 604.47 0.15 369 105 186 81 309.50 7.15 370 105 235 130 199.18 2.13 371 105 239 134 190.29 2.46 372 106 109 3 867.26 3.47 373 106 110 4 795.01 4.82 374 106 112 6 690.02 42.88 375 108 165 57 311.00 6.81 376 108 184 76 309.99 6.99 377 108 185 77 309.90 6.99 378 108 186 78 309.53 7.16 379 109 186 77 309.54 7.16 380 109 232 123 205.60 0.85 381 110 186 76 309.51 7.16 382 110 232 122 204.69 0.45 383 111 186 75 309.53 7.17 384 111 232 121 204.69 0.45 385 111 235 124 199.27 2.14 386 111 236 125 199.05 2.10 387 113 235 122 199.27 2.15 388 113 236 123 198.81 2.03 389 113 239 126 190.41 2.48 390 113 240 127 190.21 2.50 391 114 240 126 190.28 2.46 392 115 119 4 1071.00 0.04 393 116 240 124 190.35 2.48 394 116 244 128 181.69 0.67 395 116 247 131 180.90 0.62 396 117 247 130 180.92 0.61 397 118 247 129 180.93 0.61 398 119 243 124 182.02 0.73 399 119 244 125 181.69 0.71 400 119 247 128 180.78 0.66 401 126 130 4 891.07 0.85 402 127 130 3 940.24 4.52 403 130 144 14 348.47 4.63 404 132 140 8 348.93 4.61 405 132 141 9 348.71 4.67 406 132 144 12 348.51 4.63 407 132 145 13 348.51 4.63 408 133 136 3 871.15 3.89 409 133 140 7 359.85 10.04 410 134 175 41 313.16 6.74 411 134 205 71 291.00 6.86 412 134 206 72 290.95 6.83 413 134 207 73 290.07 7.17 414 135 175 40 313.18 6.74 415 135 206 71 290.97 6.84 416 136 140 4 854.74 3.15 417 136 207 71 290.93 6.83 418 137 140 3 879.06 5.09 419 137 141 4 846.69 0.90 420 137 210 73 287.69 7.31 421 138 141 3 859.10 0.96 422 138 142 4 846.69 0.90 423 138 210 72 287.40 7.47 424 138 214 76 275.64 8.16 425 138 217 79 225.69 2.00 426 139 142 3 859.74 2.57 427 139 143 4 844.88 1.79 428 140 143 3 861.02 6.03 429 140 144 4 837.15 1.86 430 141 144 3 854.87 0.92 431 141 145 4 824.32 4.53 432 141 156 15 347.12 4.37 433 141 207 66 288.91 6.79 434 141 210 69 287.39 7.46 435 141 211 70 287.34 7.49 436 141 217 76 225.14 2.27 437 142 146 4 767.48 2.06 438 142 147 5 767.48 2.06 439 142 153 11 348.62 4.83 440 142 156 14 347.64 4.35 441 142 214 72 276.70 8.52 442 142 216 74 261.04 12.97 443 142 217 75 225.14 2.27 444 143 147 4 767.46 2.07 445 143 148 5 767.46 2.06 446 144 148 4 767.45 2.06 447 144 149 5 767.42 2.08 448 145 149 4 698.04 32.56 449 145 150 5 697.99 32.53 450 145 151 6 679.35 23.29 451 145 156 11 348.16 4.88 452 145 159 14 347.89 5.00 453 146 150 4 697.86 32.53 454 146 151 5 679.40 23.31 455 149 242 93 190.26 2.44 456 149 245 96 181.69 0.72 457 151 155 4 849.72 1.95 458 151 159 8 778.24 2.11 459 151 241 90 192.19 2.42 460 151 242 91 190.91 2.73 461 151 245 94 183.13 0.41 462 152 155 3 855.30 4.73 463 152 156 4 852.87 3.52 464 152 241 89 194.28 1.73 465 153 156 3 856.06 5.11 466 153 157 4 856.11 5.14 467 153 216 63 225.02 2.31 468 153 217 64 225.02 2.32 469 153 220 67 220.56 2.29 470 154 157 3 875.83 3.00 471 154 158 4 856.10 5.15 472 154 220 66 220.53 2.31 473 154 224 70 216.43 1.61 474 154 234 80 205.24 0.55 475 155 158 3 869.95 1.78 476 155 159 4 855.73 5.28 477 155 238 83 196.51 2.85 478 155 241 86 191.97 2.31 479 156 159 3 876.05 3.17 480 156 160 4 855.73 5.28 481 156 211 55 285.22 7.63 482 156 217 61 224.99 2.31 483 157 160 3 874.38 4.00 484 157 161 4 846.57 0.77 485 157 191 34 308.13 6.83 486 157 211 54 283.10 8.66 487 157 217 60 223.52 3.01 488 157 220 63 219.72 1.90 489 157 221 64 219.44 1.79 490 157 224 67 215.16 1.11 491 158 161 3 859.69 3.85 492 158 224 66 213.47 0.65 493 158 231 73 205.83 0.76 494 158 234 76 203.93 0.38 495 158 235 77 199.19 2.12 496 158 238 80 195.16 2.17 497 159 162 3 867.10 0.14 498 159 235 76 199.16 2.12 499 159 238 79 191.97 2.31 500 159 239 80 191.18 2.60 501 159 242 83 186.66 3.14 502 160 163 3 885.35 4.61 503 160 190 30 308.90 7.13 504 160 211 51 280.44 9.98 505 161 164 3 874.31 4.09 506 161 190 29 308.19 6.84 507 161 191 30 308.18 6.85 508 161 231 70 205.82 0.77 509 162 165 3 894.30 1.04 510 162 186 24 310.19 7.04 511 162 235 73 199.12 2.11 512 163 179 16 311.53 7.21 513 163 190 27 308.50 6.71 514 163 205 42 291.56 6.73 515 163 208 45 287.15 7.05 516 164 180 16 311.33 7.31 517 164 185 21 310.43 6.95 518 164 186 22 310.07 6.94 519 164 187 23 309.96 6.99 520 164 190 26 307.66 6.96 521 165 183 18 310.42 6.94 522 165 184 19 310.41 6.94 523 165 185 20 310.33 6.94 524 165 186 21 309.96 6.99 525 166 178 12 314.45 6.62 526 166 179 13 314.45 6.61 527 166 183 17 310.41 6.95 528 167 177 10 314.48 6.61 529 167 178 11 314.45 6.62 530 167 179 12 314.45 6.62 531 168 173 5 553.37 90.34 532 168 175 7 315.03 6.58 533 168 176 8 314.67 6.58 534 168 177 9 314.54 6.64 535 168 179 11 314.54 6.64 536 168 203 35 292.74 6.88 537 169 172 3 854.92 5.87 538 169 173 4 793.08 8.48 539 169 174 5 347.12 9.37 540 169 175 6 316.22 6.04 541 169 176 7 314.75 6.55 542 170 174 4 341.31 6.50 543 170 175 5 341.31 6.50 544 170 176 6 316.20 6.04 545 175 203 28 292.79 6.86 546 175 205 30 292.42 6.75 547 175 206 31 292.43 6.75 548 177 203 26 292.75 6.88 549 178 183 5 310.48 6.92 550 178 201 23 292.81 6.86 551 178 202 24 292.80 6.86 552 178 203 25 292.80 6.86 553 179 190 11 309.19 7.03 554 179 193 14 306.61 6.61 555 179 202 23 292.81 6.86 556 179 203 24 292.75 6.88 557 179 205 26 292.75 6.88 558 179 208 29 287.36 7.07 559 180 183 3 867.73 0.70 560 180 189 9 309.93 6.98 561 180 190 10 309.43 7.12 562 180 193 13 306.61 6.61 563 180 202 22 292.81 6.87 564 181 199 18 292.88 6.87 565 181 202 21 292.81 6.87 566 182 185 3 867.88 1.05 567 186 231 45 205.79 0.78 568 186 232 46 205.49 0.70 569 186 235 49 199.24 2.15 570 187 228 41 205.82 0.77 571 187 231 44 205.81 0.77 572 187 232 45 205.69 0.79 573 188 191 3 881.13 0.04 574 188 192 4 854.62 5.01 575 188 224 36 210.09 0.53 576 188 225 37 209.96 0.46 577 188 228 40 205.81 0.77 578 188 231 43 205.82 0.78 579 189 192 3 858.65 3.00 580 189 193 4 854.62 5.01 581 190 193 3 867.38 0.64 582 190 194 4 854.62 5.01 583 190 208 18 287.31 7.07 584 190 211 21 284.92 7.65 585 191 194 3 856.36 6.15 586 191 195 4 854.34 5.13 587 191 221 30 218.48 1.96 588 191 224 33 213.51 0.62 589 191 225 34 212.86 0.95 590 191 231 40 205.86 0.77 591 192 195 3 868.97 0.15 592 192 196 4 847.88 1.92 593 192 225 33 212.95 0.90 594 193 196 3 863.55 2.87 595 193 197 4 847.88 1.92 596 193 202 9 292.86 6.87 597 193 204 11 292.79 6.89 598 194 197 3 875.61 2.79 599 194 204 10 292.78 6.90 600 194 208 14 288.93 7.85 601 194 209 15 287.31 7.11 602 194 211 17 286.44 6.90 603 194 212 18 280.33 9.90 604 194 221 27 219.67 1.85 605 195 212 17 284.52 7.81 606 195 218 23 223.56 3.01 607 195 221 26 219.71 1.84 608 195 222 27 219.66 1.84 609 195 225 30 213.71 0.65 610 197 204 7 292.89 6.89 611 198 204 6 292.90 6.89 612 198 209 11 290.92 6.88 613 199 202 3 875.10 3.15 614 204 208 4 839.16 2.08 615 204 209 5 823.72 9.77 616 205 208 3 854.00 5.66 617 205 209 4 854.77 5.26 618 206 209 3 855.93 5.83 619 206 210 4 854.89 5.20 620 207 210 3 865.98 0.34 621 207 211 4 856.49 5.11 622 208 211 3 890.48 3.71 623 209 212 3 871.39 1.80 624 210 213 3 878.19 5.20 625 210 214 4 822.36 4.33 626 211 217 6 223.58 3.00 627 211 218 7 223.56 3.01 628 211 221 10 219.81 1.92 629 212 218 6 223.56 3.01 630 212 221 9 221.28 2.06 631 214 217 3 869.09 0.68 632 214 218 4 813.82 9.78 633 215 219 4 869.06 0.68 634 216 219 3 903.82 4.29 635 216 220 4 869.06 0.68 636 217 220 3 871.00 1.66 637 217 221 4 858.84 4.42 638 218 221 3 869.06 0.69 639 218 222 4 856.06 5.82 640 219 222 3 859.04 3.39 641 219 223 4 855.05 5.33 642 220 223 3 853.82 1.20 643 220 224 4 850.12 3.04 644 221 224 3 866.66 2.15 645 221 225 4 850.12 3.04 646 222 225 3 861.09 3.10 647 222 226 4 841.14 1.50 648 223 226 3 860.11 4.83 649 223 227 4 839.64 2.24 650 223 230 7 735.91 16.89 651 223 234 11 671.51 2.10 652 224 227 3 860.95 11.39 653 224 230 6 700.28 2.90 654 224 231 7 671.28 2.22 655 224 234 10 671.29 2.22 656 227 230 3 864.65 4.04 657 227 231 4 842.14 2.52 658 228 231 3 858.80 6.99 659 228 232 4 842.12 2.52 660 229 232 3 869.73 1.52 661 229 233 4 851.90 7.39 662 230 233 3 874.69 4.00 663 230 234 4 869.73 1.52 664 231 234 3 874.69 4.00 665 231 235 4 840.47 3.34 666 232 235 3 858.91 3.89 667 232 236 4 839.66 2.94 668 233 236 3 849.21 0.95 669 233 237 4 847.81 1.06 670 234 237 3 859.18 5.21 671 234 238 4 847.81 1.06 672 235 238 3 888.00 9.21 673 235 239 4 847.80 1.06 674 236 239 3 857.09 3.38 675 236 240 4 839.74 2.46 676 237 240 3 872.89 6.08 677 237 241 4 852.85 4.09 678 238 241 3 850.22 5.41 679 238 242 4 820.80 8.12 680 239 242 3 858.01 4.26 681 239 243 4 820.77 8.14 682 240 243 3 859.77 4.27 683 240 244 4 843.26 3.11 684 241 244 3 857.86 3.31 685 241 245 4 841.19 4.15 686 242 245 3 864.31 1.90 687 244 247 3 793.70 4.54