BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 232 230 22.51 2.10 2 3 42 39 36.54 0.80 3 4 7 3 844.07 6.84 4 4 8 4 609.69 8.49 5 4 34 30 37.23 0.95 6 4 38 34 36.61 0.79 7 4 232 228 36.91 0.94 8 5 8 3 847.36 5.20 9 5 38 33 37.07 0.99 10 5 42 37 36.79 0.88 11 5 228 223 36.91 0.94 12 6 38 32 37.08 0.99 13 6 41 35 37.05 0.98 14 6 42 36 36.94 0.95 15 7 34 27 37.48 1.06 16 7 37 30 37.24 0.95 17 7 38 31 37.02 0.99 18 8 34 26 38.26 1.27 19 10 34 24 40.41 2.00 20 13 16 3 834.11 2.66 21 13 17 4 829.84 4.79 22 13 33 20 62.77 2.20 23 14 17 3 835.14 2.15 24 14 20 6 602.11 3.38 25 15 21 6 591.38 0.52 26 15 130 115 95.06 0.22 27 15 133 118 95.06 0.22 28 15 134 119 94.85 0.14 29 16 22 6 423.87 83.20 30 16 33 17 94.12 0.39 31 16 64 48 94.16 0.38 32 16 68 52 94.03 0.43 33 16 71 55 94.49 0.29 34 16 72 56 94.48 0.30 35 16 134 118 95.06 0.22 36 17 20 3 853.62 3.15 37 17 64 47 94.86 0.13 38 18 23 5 593.81 1.77 39 18 24 6 105.95 0.42 40 18 30 12 94.42 0.32 41 18 64 46 94.98 0.20 42 19 22 3 838.02 1.76 43 19 24 5 117.88 3.18 44 19 112 93 128.41 0.29 45 20 112 92 128.41 0.29 46 20 127 107 123.28 2.26 47 20 130 110 123.27 2.26 48 21 99 78 122.84 2.40 49 21 105 84 123.17 2.29 50 21 108 87 123.39 2.41 51 21 127 106 123.42 2.25 52 21 130 109 123.27 2.26 53 21 131 110 123.20 2.29 54 21 134 113 123.21 2.27 55 22 64 42 121.39 3.04 56 22 67 45 121.43 3.02 57 22 68 46 121.41 3.03 58 22 71 49 123.08 2.28 59 22 96 74 121.45 3.02 60 22 99 77 122.90 2.42 61 23 92 69 123.05 2.50 62 23 95 72 123.09 2.52 63 23 96 73 122.89 2.42 64 23 99 76 123.46 2.33 65 23 108 85 130.75 1.33 66 25 28 3 854.98 3.12 67 25 92 67 136.43 1.21 68 28 62 34 140.40 0.41 69 28 88 60 140.84 0.30 70 28 89 61 139.64 0.34 71 28 92 64 141.08 0.38 72 29 178 149 141.26 0.28 73 30 64 34 141.56 0.40 74 31 35 4 854.28 5.58 75 31 36 5 835.22 1.17 76 31 39 8 822.40 2.55 77 31 64 33 148.24 1.61 78 31 65 34 141.84 0.26 79 31 175 144 141.59 0.39 80 31 213 182 148.04 1.52 81 31 235 204 158.81 1.14 82 32 35 3 854.28 5.58 83 32 36 4 835.35 1.18 84 32 235 203 161.93 0.28 85 33 36 3 854.28 5.58 86 33 37 4 835.38 1.16 87 33 64 31 164.83 0.58 88 33 68 35 163.93 0.38 89 33 72 39 181.03 0.85 90 34 37 3 861.34 2.06 91 34 38 4 835.40 1.14 92 35 38 3 840.62 1.24 93 35 39 4 840.34 1.38 94 35 230 195 165.83 1.57 95 35 232 197 166.22 1.70 96 35 235 200 175.92 0.10 97 36 39 3 860.82 0.27 98 36 40 4 836.07 3.51 99 36 65 29 182.81 1.12 100 36 68 32 183.00 1.13 101 36 69 33 182.95 1.14 102 37 40 3 853.63 3.86 103 37 41 4 835.33 3.88 104 37 69 32 183.00 1.13 105 37 72 35 183.53 1.35 106 37 73 36 183.09 1.16 107 38 41 3 862.84 0.74 108 38 42 4 836.74 4.59 109 38 230 192 182.43 1.03 110 38 232 194 183.51 1.45 111 39 42 3 849.18 1.62 112 39 43 4 833.13 1.74 113 39 173 134 183.16 1.21 114 39 211 172 182.57 0.98 115 39 213 174 183.16 1.21 116 39 237 198 182.58 0.99 117 39 239 200 182.29 0.85 118 40 43 3 841.26 2.33 119 40 44 4 833.20 1.71 120 40 55 15 183.50 1.36 121 40 69 29 184.27 1.52 122 40 73 33 184.30 1.53 123 40 76 36 184.23 1.51 124 40 77 37 184.10 1.44 125 40 173 133 183.20 1.22 126 41 44 3 834.03 1.29 127 41 45 4 833.20 1.71 128 41 73 32 184.30 1.53 129 42 45 3 853.69 5.86 130 42 46 4 836.79 0.58 131 42 228 186 183.96 1.57 132 42 230 188 184.16 1.52 133 42 239 197 183.81 1.48 134 43 46 3 853.71 5.86 135 43 47 4 842.51 0.27 136 43 171 128 183.97 1.42 137 43 209 166 183.79 1.49 138 43 239 196 183.81 1.48 139 44 47 3 857.44 4.07 140 44 53 9 184.20 1.51 141 44 76 32 184.30 1.52 142 44 171 127 184.17 1.52 143 46 209 163 184.16 1.51 144 46 227 181 184.16 1.52 145 46 228 182 184.18 1.51 146 46 241 195 184.00 1.49 147 47 53 6 417.53 84.48 148 47 170 123 184.19 1.50 149 47 171 124 184.23 1.52 150 47 207 160 184.12 1.51 151 47 209 162 184.17 1.51 152 48 207 159 184.17 1.51 153 52 76 24 815.36 0.82 154 52 77 25 815.34 0.81 155 52 78 26 815.32 0.82 156 53 76 23 815.45 0.84 157 53 77 24 815.38 0.82 158 53 78 25 815.37 0.82 159 53 79 26 815.37 0.83 160 53 170 117 468.69 1.46 161 53 171 118 468.66 1.45 162 54 78 24 815.43 0.83 163 54 79 25 815.37 0.83 164 54 81 27 815.35 0.83 165 54 167 113 468.99 1.39 166 54 169 115 468.71 1.46 167 54 170 116 468.73 1.45 168 54 171 117 468.69 1.46 169 55 66 11 815.45 0.83 170 55 69 14 815.56 0.83 171 55 77 22 815.49 0.85 172 55 79 24 815.45 0.83 173 55 80 25 815.37 0.83 174 55 81 26 815.36 0.83 175 55 171 116 468.70 1.46 176 55 173 118 468.68 1.46 177 56 81 25 815.36 0.83 178 56 83 27 815.35 0.83 179 56 160 104 469.81 1.31 180 56 163 107 469.68 1.25 181 56 164 108 469.58 1.29 182 56 169 113 468.95 1.38 183 56 171 115 468.79 1.44 184 56 172 116 468.69 1.46 185 56 173 117 468.70 1.46 186 56 200 144 335.11 0.32 187 56 204 148 334.92 0.32 188 57 60 3 845.35 4.92 189 57 65 8 815.65 0.84 190 57 66 9 815.57 0.83 191 57 81 24 815.45 0.84 192 57 82 25 815.37 0.83 193 57 83 26 815.35 0.83 194 57 173 116 468.73 1.45 195 57 174 117 468.65 1.44 196 58 83 25 815.35 0.83 197 58 84 26 815.34 0.83 198 58 154 96 469.74 1.11 199 58 172 114 469.00 1.40 200 58 174 116 468.75 1.45 201 58 200 142 335.23 0.28 202 59 83 24 815.45 0.84 203 59 84 25 815.34 0.83 204 59 154 95 516.00 0.31 205 59 174 115 468.77 1.43 206 59 177 118 467.71 1.33 207 60 63 3 843.36 5.83 208 60 82 22 815.50 0.84 209 60 83 23 815.48 0.83 210 60 84 24 815.45 0.84 211 60 90 30 596.12 7.17 212 60 93 33 576.75 2.48 213 61 84 23 815.48 0.83 214 61 89 28 621.37 19.78 215 61 93 32 576.75 2.48 216 62 89 27 621.36 19.78 217 62 92 30 576.76 2.48 218 62 93 31 576.66 2.52 219 62 96 34 576.66 2.52 220 63 66 3 839.88 1.42 221 63 67 4 820.25 0.60 222 63 93 30 576.49 2.47 223 63 96 33 576.50 2.47 224 64 67 3 842.23 5.01 225 64 68 4 825.62 3.29 226 64 96 32 576.46 2.48 227 65 68 3 850.65 0.81 228 65 69 4 842.24 5.01 229 65 173 108 468.78 1.43 230 65 174 109 468.72 1.45 231 65 175 110 468.63 1.44 232 66 69 3 847.70 2.28 233 66 70 4 835.27 0.46 234 66 80 14 815.56 0.86 235 66 81 15 815.55 0.86 236 66 82 16 815.50 0.84 237 66 97 31 574.45 1.78 238 67 70 3 840.59 1.27 239 67 71 4 835.27 0.46 240 67 96 29 574.93 2.03 241 67 97 30 574.40 1.77 242 67 99 32 574.25 1.75 243 67 100 33 574.12 1.81 244 68 71 3 850.55 3.29 245 68 72 4 834.58 0.82 246 69 72 3 840.10 1.94 247 69 73 4 818.56 1.23 248 69 77 8 815.81 0.84 249 69 173 104 468.71 1.45 250 70 74 4 815.84 0.84 251 70 77 7 815.75 0.83 252 70 100 30 573.64 1.60 253 70 145 75 563.52 0.59 254 71 99 28 574.21 1.71 255 71 100 29 574.14 1.71 256 71 105 34 573.50 1.58 257 71 134 63 567.22 1.07 258 71 138 67 567.08 1.12 259 73 76 3 858.48 4.70 260 73 77 4 827.92 3.14 261 74 77 3 856.64 3.78 262 74 138 64 567.03 1.10 263 74 141 67 565.15 0.78 264 74 142 68 565.14 0.78 265 74 143 69 565.10 0.76 266 74 144 70 565.03 0.73 267 74 145 71 563.47 0.59 268 75 141 66 565.16 0.77 269 77 80 3 931.23 6.12 270 77 145 68 563.18 0.58 271 78 145 67 563.19 0.58 272 78 146 68 563.16 0.58 273 79 145 66 563.25 0.60 274 79 146 67 563.16 0.57 275 79 167 88 468.98 1.38 276 80 97 17 574.09 1.63 277 80 100 20 573.56 1.64 278 80 145 65 563.33 0.60 279 80 146 66 563.18 0.58 280 80 147 67 563.07 0.58 281 80 148 68 563.09 0.57 282 81 148 67 563.09 0.57 283 81 163 82 469.62 1.28 284 81 164 83 469.49 1.31 285 81 167 86 469.19 1.36 286 82 97 15 574.20 1.72 287 82 148 66 563.13 0.56 288 82 149 67 562.96 0.62 289 82 150 68 562.82 0.56 290 83 150 67 562.82 0.55 291 83 163 80 469.74 1.24 292 84 90 6 598.45 6.00 293 84 150 66 563.09 0.56 294 84 151 67 562.77 0.55 295 84 152 68 562.68 0.55 296 85 151 66 562.82 0.55 297 85 152 67 562.81 0.55 298 85 153 68 562.69 0.54 299 85 154 69 562.38 0.64 300 86 89 3 889.28 1.45 301 86 90 4 849.03 1.16 302 87 90 3 861.07 1.60 303 87 91 4 849.02 1.16 304 88 91 3 868.39 1.06 305 89 92 3 854.42 1.54 306 89 93 4 833.33 0.73 307 90 93 3 847.38 4.70 308 90 94 4 834.49 0.95 309 90 149 59 563.35 0.53 310 90 150 60 563.25 0.56 311 90 151 61 563.18 0.57 312 91 94 3 868.38 6.62 313 91 95 4 856.08 0.47 314 92 95 3 856.09 0.47 315 92 96 4 850.25 3.29 316 93 96 3 851.02 2.06 317 93 97 4 845.09 0.71 318 93 149 56 563.36 0.52 319 94 97 3 858.54 0.74 320 94 98 4 845.09 0.71 321 94 149 55 563.41 0.55 322 95 98 3 858.55 0.74 323 95 99 4 847.84 0.67 324 96 99 3 854.19 1.40 325 97 100 3 848.89 0.15 326 97 147 50 563.55 0.59 327 97 149 52 563.35 0.52 328 98 101 3 873.24 8.64 329 98 104 6 581.14 6.08 330 99 104 5 613.07 9.31 331 99 105 6 581.73 6.37 332 99 108 9 581.15 6.08 333 100 138 38 567.20 1.15 334 100 144 44 564.02 0.68 335 100 145 45 563.93 0.65 336 100 147 47 563.53 0.58 337 101 104 3 852.60 1.53 338 101 105 4 844.40 6.27 339 101 138 37 567.33 1.20 340 101 144 43 564.03 0.68 341 102 105 3 856.44 0.26 342 102 106 4 804.08 26.43 343 102 135 33 567.55 1.09 344 102 138 36 567.38 1.17 345 102 139 37 567.28 1.17 346 102 143 41 564.99 0.71 347 102 144 42 564.95 0.72 348 103 106 3 850.39 3.27 349 103 107 4 805.22 25.86 350 104 107 3 851.53 2.71 351 104 108 4 850.39 3.27 352 105 108 3 846.53 4.33 353 105 109 4 794.61 21.66 354 105 131 26 567.87 1.08 355 105 134 29 567.83 1.10 356 105 135 30 567.66 1.16 357 105 138 33 567.65 1.17 358 106 109 3 850.49 2.45 359 106 110 4 803.18 17.37 360 106 135 29 567.87 1.09 361 107 110 3 844.78 1.94 362 107 111 4 837.17 0.12 363 108 111 3 841.23 2.14 364 108 112 4 841.24 2.16 365 109 112 3 849.36 1.91 366 109 113 4 841.24 2.16 367 109 128 19 568.06 1.11 368 109 131 22 567.98 1.12 369 109 132 23 567.98 1.12 370 109 135 26 567.98 1.12 371 110 113 3 846.99 0.77 372 110 114 4 841.24 2.16 373 111 114 3 848.24 0.14 374 111 115 4 841.23 2.15 375 112 115 3 846.99 0.76 376 112 116 4 837.19 0.14 377 112 124 12 568.47 1.06 378 112 127 15 568.16 1.15 379 112 128 16 568.12 1.14 380 113 116 3 845.79 1.56 381 113 117 4 837.37 0.29 382 113 125 12 568.46 1.09 383 113 128 15 568.15 1.14 384 114 117 3 839.43 1.32 385 114 118 4 838.06 0.65 386 115 118 3 841.86 1.25 387 115 119 4 832.75 1.99 388 115 124 9 568.54 1.09 389 116 119 3 845.15 0.09 390 116 120 4 829.56 3.56 391 116 124 8 568.54 1.08 392 116 125 9 568.55 1.09 393 119 124 5 744.24 1.54 394 121 124 3 865.12 1.88 395 122 125 3 862.91 3.75 396 122 126 4 841.97 1.50 397 123 126 3 851.56 1.92 398 123 127 4 798.63 23.16 399 124 127 3 842.43 2.66 400 124 128 4 836.69 4.12 401 125 128 3 834.64 3.10 402 125 129 4 842.32 6.94 403 126 129 3 856.48 5.17 404 126 130 4 834.61 3.02 405 127 130 3 842.87 6.68 406 127 131 4 846.37 4.92 407 128 131 3 848.95 3.62 408 128 132 4 836.88 0.18 409 129 132 3 860.07 3.58 410 129 133 4 841.59 2.18 411 130 133 3 847.11 0.57 412 130 134 4 845.61 0.18 413 131 134 3 847.07 0.55 414 131 135 4 847.07 0.55 415 132 135 3 854.71 3.27 416 132 136 4 847.10 0.54 417 133 136 3 847.10 0.54 418 133 137 4 845.66 0.18 419 134 137 3 847.12 0.52 420 134 138 4 845.66 0.18 421 135 138 3 845.65 0.18 422 135 139 4 845.65 0.18 423 136 139 3 845.64 0.19 424 136 140 4 845.55 0.18 425 137 140 3 845.59 0.16 426 137 141 4 845.55 0.17 427 138 141 3 852.39 1.36 428 138 142 4 845.48 0.21 429 138 143 5 726.45 3.49 430 138 144 6 698.64 17.39 431 139 142 3 850.50 2.31 432 139 143 4 657.77 3.05 433 150 162 12 469.95 1.26 434 150 163 13 469.72 1.35 435 150 165 15 469.79 1.31 436 152 159 7 470.20 1.18 437 152 163 11 469.97 1.26 438 153 156 3 781.84 24.11 439 153 159 6 470.35 1.18 440 153 160 7 470.36 1.17 441 154 160 6 470.37 1.17 442 154 196 42 335.76 0.21 443 154 199 45 335.63 0.24 444 154 200 46 335.58 0.26 445 155 180 25 464.48 1.25 446 155 192 37 336.06 0.24 447 155 196 41 335.75 0.21 448 155 199 44 335.71 0.23 449 156 159 3 860.95 2.06 450 156 160 4 834.66 2.19 451 157 160 3 838.67 4.02 452 157 199 42 335.44 0.29 453 157 202 45 335.14 0.28 454 158 161 3 863.99 0.54 455 159 162 3 860.83 1.04 456 159 163 4 840.50 0.09 457 160 163 3 863.05 3.66 458 160 164 4 838.14 0.48 459 160 199 39 335.37 0.37 460 160 200 40 335.19 0.30 461 160 203 43 334.81 0.34 462 161 164 3 865.60 2.38 463 161 165 4 839.90 0.39 464 162 165 3 843.98 1.82 465 164 167 3 783.13 23.30 466 164 169 5 604.88 0.85 467 164 203 39 334.79 0.35 468 168 205 37 334.29 0.35 469 169 172 3 895.66 2.33 470 169 204 35 334.37 0.37 471 169 205 36 334.39 0.39 472 169 208 39 334.01 0.40 473 170 207 37 334.25 0.33 474 170 208 38 333.96 0.42 475 170 209 39 333.96 0.42 476 171 209 38 333.94 0.41 477 172 200 28 335.06 0.34 478 172 204 32 334.93 0.33 479 172 209 37 334.11 0.39 480 172 210 38 333.88 0.40 481 172 211 39 333.88 0.41 482 173 211 38 333.88 0.41 483 174 211 37 334.01 0.41 484 174 212 38 333.81 0.42 485 174 213 39 333.82 0.41 486 175 213 38 333.82 0.41 487 175 235 60 234.35 2.62 488 176 181 5 780.39 22.12 489 176 188 12 337.04 0.50 490 176 196 20 335.74 0.24 491 176 212 36 334.24 0.38 492 176 213 37 333.93 0.40 493 176 214 38 333.87 0.41 494 177 180 3 861.83 3.70 495 177 181 4 832.96 4.17 496 177 188 11 337.81 0.36 497 178 181 3 834.44 2.16 498 178 184 6 340.01 0.69 499 178 235 57 241.20 4.21 500 180 188 8 337.86 0.35 501 180 189 9 337.86 0.35 502 181 187 6 338.19 0.18 503 181 188 7 337.86 0.35 504 181 189 8 337.88 0.35 505 181 214 33 334.54 0.30 506 181 215 34 334.51 0.31 507 182 187 5 338.24 0.20 508 182 189 7 337.90 0.35 509 183 186 3 845.87 3.21 510 187 214 27 334.56 0.29 511 187 216 29 334.12 0.31 512 187 220 33 331.79 0.24 513 188 192 4 846.25 3.71 514 188 193 5 775.38 24.41 515 188 214 26 334.53 0.30 516 189 192 3 868.09 6.04 517 189 193 4 829.59 2.69 518 190 193 3 850.55 1.55 519 190 194 4 829.53 2.66 520 190 220 30 273.78 10.27 521 191 194 3 861.45 2.73 522 191 195 4 845.84 0.80 523 192 195 3 845.85 0.80 524 192 196 4 830.62 2.14 525 193 196 3 839.62 2.31 526 193 197 4 839.63 2.37 527 193 219 26 273.80 10.29 528 193 220 27 273.70 10.29 529 194 197 3 839.62 2.38 530 194 198 4 829.99 2.44 531 195 198 3 839.61 2.39 532 195 199 4 829.84 2.36 533 196 199 3 835.27 5.08 534 196 200 4 830.15 2.52 535 197 200 3 836.27 4.58 536 197 201 4 829.49 2.52 537 197 210 13 334.68 0.35 538 197 240 43 218.84 1.21 539 197 245 48 218.03 1.28 540 198 202 4 827.71 1.57 541 198 245 47 218.01 1.10 542 198 249 51 209.58 1.44 543 198 250 52 200.96 1.24 544 199 202 3 848.43 6.83 545 200 203 3 861.41 0.45 546 200 204 4 762.31 31.13 547 200 210 10 334.60 0.34 548 201 204 3 829.43 2.43 549 201 210 9 334.60 0.34 550 201 242 41 218.23 1.20 551 201 245 44 217.31 1.05 552 201 246 45 216.59 1.35 553 202 246 44 216.13 1.13 554 204 208 4 742.86 39.50 555 204 210 6 456.38 58.45 556 204 242 38 218.21 1.20 557 205 208 3 851.45 1.72 558 205 242 37 218.05 1.11 559 206 241 35 218.42 1.21 560 206 242 36 218.21 1.19 561 206 243 37 217.92 1.07 562 207 241 34 218.38 1.23 563 208 241 33 218.25 1.19 564 208 242 34 218.12 1.15 565 209 239 30 218.55 1.19 566 209 240 31 218.48 1.20 567 209 241 32 218.27 1.19 568 210 238 28 218.84 1.21 569 210 239 29 218.59 1.17 570 210 240 30 218.50 1.19 571 210 242 32 218.36 1.24 572 211 238 27 218.84 1.21 573 211 239 28 218.68 1.22 574 212 219 7 287.90 24.14 575 212 237 25 218.88 1.21 576 212 238 26 218.87 1.22 577 213 219 6 295.39 21.17 578 213 235 22 232.69 1.89 579 213 236 23 228.10 0.14 580 213 237 24 218.87 1.22 581 214 219 5 456.87 59.36 582 214 235 21 235.66 2.54 583 214 236 22 228.54 0.06 584 215 234 19 240.55 4.41 585 216 219 3 895.26 1.76 586 216 234 18 241.22 4.20 587 216 236 20 240.62 4.42 588 217 231 14 241.02 4.23 589 217 233 16 241.31 4.20 590 217 236 19 240.94 4.27 591 218 229 11 241.13 4.18 592 218 236 18 241.19 4.19 593 219 229 10 241.24 4.21 594 219 236 17 241.31 4.18 595 219 237 18 241.28 4.18 596 219 238 19 241.13 4.17 597 221 224 3 871.95 3.83 598 221 226 5 608.21 16.30 599 221 229 8 284.97 25.72 600 221 238 17 241.33 4.18 601 221 240 19 241.21 4.21 602 223 248 25 219.03 1.66 603 224 240 16 241.36 4.19 604 224 245 21 225.53 1.59 605 224 248 24 218.41 1.97 606 226 229 3 915.37 0.83 607 226 238 12 241.81 4.26 608 226 239 13 241.80 4.25 609 226 240 14 241.62 4.19 610 227 239 12 241.82 4.25 611 227 240 13 241.80 4.25 612 227 241 14 241.77 4.24 613 228 238 10 242.09 4.38 614 228 239 11 241.88 4.28 615 229 237 8 242.29 4.46 616 229 238 9 242.10 4.38 617 230 236 6 242.69 4.38 618 230 237 7 242.56 4.33 619 231 235 4 470.46 65.53 620 231 236 5 242.69 4.38 621 232 235 3 777.95 19.87 622 240 245 5 790.86 23.82 623 241 244 3 858.49 0.64 624 241 245 4 799.56 19.54 625 242 245 3 854.72 1.25 626 242 246 4 838.43 0.10 627 243 246 3 849.97 1.13 628 243 247 4 840.23 1.00 629 244 247 3 847.50 0.11 630 244 248 4 838.45 0.11 631 245 248 3 858.41 2.27 632 245 249 4 799.55 19.56 633 246 249 3 838.98 0.41