BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 116.14 0.21 2 3 204 201 116.09 0.19 3 3 205 202 115.98 0.23 4 3 224 221 116.07 0.21 5 3 225 222 116.02 0.23 6 3 227 224 115.89 0.26 7 4 34 30 729.79 32.79 8 4 35 31 729.77 32.77 9 4 36 32 729.73 32.77 10 4 37 33 729.72 32.77 11 4 38 34 729.72 32.77 12 4 205 201 116.10 0.20 13 5 37 32 729.73 32.77 14 5 38 33 729.73 32.77 15 5 221 216 116.02 0.22 16 5 226 221 116.12 0.21 17 5 227 222 116.03 0.22 18 5 247 242 17.91 0.76 19 6 37 31 729.75 32.77 20 6 38 32 729.73 32.77 21 6 40 34 729.73 32.77 22 6 90 84 119.09 0.14 23 6 124 118 116.56 0.22 24 6 161 155 116.56 0.23 25 6 205 199 116.50 0.24 26 6 207 201 116.09 0.21 27 6 227 221 116.15 0.22 28 6 228 222 116.02 0.21 29 6 229 223 115.98 0.24 30 7 37 30 729.81 32.77 31 7 38 31 729.75 32.77 32 7 39 32 729.73 32.77 33 7 40 33 729.73 32.77 34 7 229 222 116.04 0.21 35 7 240 233 51.19 0.52 36 7 243 236 44.13 0.09 37 7 244 237 37.36 0.09 38 7 247 240 19.75 0.57 39 8 40 32 729.73 32.77 40 8 42 34 729.72 32.77 41 8 229 221 116.14 0.21 42 8 230 222 115.98 0.24 43 8 231 223 115.94 0.23 44 8 240 232 51.20 0.52 45 9 40 31 729.77 32.77 46 9 41 32 729.73 32.77 47 9 42 33 729.73 32.77 48 9 231 222 116.02 0.22 49 9 235 226 81.62 0.65 50 9 240 231 51.20 0.52 51 10 42 32 729.73 32.77 52 10 64 54 513.76 32.57 53 10 92 82 121.42 1.01 54 10 95 85 118.36 0.04 55 10 230 220 116.45 0.23 56 10 231 221 116.10 0.21 57 10 232 222 115.96 0.24 58 10 235 225 85.17 0.31 59 11 14 3 867.37 3.00 60 11 20 9 730.97 32.86 61 11 23 12 730.93 32.83 62 11 24 13 730.92 32.83 63 11 27 16 730.85 32.87 64 11 41 30 729.81 32.77 65 11 42 31 729.75 32.79 66 11 43 32 729.73 32.77 67 11 51 40 588.15 1.54 68 11 64 53 513.95 32.59 69 11 235 224 106.52 1.09 70 11 236 225 84.48 0.65 71 12 44 32 729.73 32.77 72 12 64 52 513.93 32.58 73 12 95 83 129.99 0.61 74 12 97 85 132.31 1.53 75 12 232 220 116.43 0.24 76 13 44 31 729.75 32.78 77 14 44 30 729.77 32.79 78 14 236 222 115.73 0.19 79 15 19 4 833.70 1.42 80 15 20 5 832.66 1.89 81 15 47 32 729.56 32.84 82 16 19 3 842.41 2.77 83 16 20 4 839.40 4.27 84 17 20 3 857.31 0.55 85 17 21 4 841.37 3.29 86 17 47 30 729.62 32.84 87 17 50 33 678.21 28.69 88 18 21 3 847.51 0.22 89 18 22 4 841.32 3.23 90 19 22 3 856.51 0.94 91 19 23 4 848.35 5.03 92 20 23 3 849.00 4.70 93 20 24 4 846.14 5.57 94 20 47 27 729.52 32.83 95 20 50 30 664.04 30.28 96 20 51 31 587.68 1.90 97 20 54 34 581.51 0.70 98 21 24 3 856.65 0.89 99 21 25 4 848.25 5.08 100 21 50 29 664.04 30.28 101 21 54 33 581.58 0.73 102 22 25 3 864.30 4.71 103 22 26 4 848.99 4.72 104 23 26 3 863.05 2.31 105 23 27 4 847.74 4.79 106 23 235 212 110.16 2.75 107 23 236 213 90.81 1.99 108 23 237 214 81.84 0.69 109 23 238 215 80.42 0.04 110 24 27 3 853.50 7.67 111 24 28 4 847.74 4.79 112 24 51 27 581.12 0.98 113 24 54 30 571.72 5.29 114 24 55 31 518.62 31.29 115 25 28 3 873.38 2.27 116 25 29 4 847.75 4.79 117 26 29 3 873.38 2.27 118 26 238 212 77.60 1.04 119 26 241 215 60.08 0.96 120 27 30 3 870.72 2.51 121 27 41 14 729.91 32.76 122 27 55 28 518.58 31.35 123 27 59 32 515.60 32.10 124 27 235 208 90.83 1.99 125 27 237 210 80.51 0.04 126 27 240 213 57.45 2.29 127 27 241 214 50.94 0.43 128 28 54 26 519.34 31.48 129 28 55 27 518.54 31.33 130 28 56 28 518.50 31.34 131 28 58 30 517.94 31.36 132 28 59 31 516.91 31.64 133 30 58 28 517.62 31.23 134 30 241 211 50.96 0.42 135 30 244 214 47.36 0.56 136 30 245 215 47.09 0.68 137 30 248 218 42.73 0.59 138 31 248 217 37.49 0.07 139 32 39 7 730.95 32.59 140 32 58 26 514.86 32.24 141 32 59 27 514.85 32.24 142 32 248 216 32.15 0.46 143 33 36 3 858.77 0.35 144 33 37 4 825.19 5.58 145 34 37 3 839.67 2.36 146 34 58 24 514.17 32.52 147 37 244 207 37.86 0.15 148 37 247 210 23.75 1.31 149 37 248 211 17.84 0.76 150 38 205 167 116.60 0.22 151 39 59 20 514.17 32.54 152 39 60 21 514.14 32.52 153 39 244 205 37.53 0.00 154 40 60 20 514.15 32.52 155 40 61 21 514.14 32.52 156 40 62 22 514.12 32.54 157 40 90 50 120.39 0.50 158 41 51 10 519.74 30.77 159 41 55 14 518.10 31.47 160 41 59 18 514.63 32.37 161 41 60 19 514.56 32.38 162 41 61 20 514.14 32.52 163 41 62 21 514.14 32.52 164 42 62 20 514.14 32.53 165 42 63 21 514.06 32.56 166 42 64 22 513.94 32.59 167 42 90 48 121.64 0.83 168 42 91 49 121.66 0.83 169 42 92 50 121.67 0.87 170 42 124 82 121.45 0.76 171 43 47 4 803.96 2.79 172 43 48 5 690.54 53.50 173 43 51 8 580.96 0.99 174 43 62 19 514.41 32.46 175 43 64 21 514.10 32.54 176 44 47 3 846.69 1.12 177 44 48 4 835.95 1.14 178 44 64 20 514.01 32.57 179 45 48 3 853.38 2.42 180 47 50 3 860.26 1.02 181 47 51 4 839.76 2.90 182 48 51 3 850.90 1.69 183 48 52 4 849.88 2.21 184 48 61 13 514.92 32.44 185 48 62 14 514.87 32.45 186 48 63 15 514.72 32.39 187 48 88 40 122.00 0.85 188 49 52 3 856.41 1.05 189 49 53 4 854.70 0.22 190 50 53 3 871.74 2.50 191 50 54 4 854.70 0.22 192 51 54 3 856.28 1.01 193 51 55 4 854.71 0.22 194 52 55 3 851.75 1.89 195 52 56 4 853.22 1.16 196 52 57 5 837.94 3.83 197 52 59 7 576.43 1.78 198 52 60 8 516.83 31.57 199 52 61 9 515.04 32.38 200 55 59 4 818.61 4.11 201 55 61 6 559.31 52.92 202 56 59 3 855.72 1.02 203 59 244 185 41.90 0.35 204 60 89 29 120.88 0.68 205 60 90 30 120.45 0.59 206 61 89 28 120.99 0.68 207 61 90 29 120.46 0.59 208 62 88 26 121.27 0.71 209 62 90 28 120.52 0.57 210 63 78 15 138.59 0.51 211 63 83 20 135.53 0.18 212 63 88 25 122.11 0.89 213 63 90 27 121.70 0.85 214 63 91 28 121.69 0.84 215 63 92 29 121.74 0.88 216 64 92 28 121.69 0.88 217 65 92 27 133.15 0.93 218 65 98 33 136.63 0.17 219 65 112 47 137.97 0.47 220 66 78 12 139.19 0.74 221 66 79 13 138.64 0.47 222 66 80 14 138.37 0.45 223 66 91 25 136.72 0.14 224 66 93 27 136.73 0.14 225 66 112 46 138.05 0.48 226 66 116 50 137.92 0.49 227 66 121 55 137.91 0.49 228 67 77 10 139.71 0.81 229 67 78 11 139.28 0.77 230 67 79 12 138.65 0.48 231 67 112 45 138.28 0.56 232 68 108 40 138.30 0.57 233 68 111 43 138.30 0.57 234 68 112 44 138.30 0.57 235 69 74 5 289.75 13.87 236 69 79 10 140.16 0.69 237 70 74 4 865.79 1.91 238 70 79 9 140.28 0.64 239 71 79 8 140.82 0.42 240 71 81 10 140.36 0.65 241 71 82 11 140.82 0.42 242 74 77 3 860.41 3.62 243 78 82 4 838.23 6.25 244 79 82 3 856.49 2.88 245 79 83 4 833.29 3.60 246 80 83 3 844.43 1.96 247 80 84 4 838.56 3.83 248 80 115 35 270.08 19.14 249 80 116 36 269.98 19.09 250 80 119 39 329.26 8.68 251 80 121 41 329.23 8.69 252 81 84 3 849.57 0.59 253 81 85 4 849.56 0.59 254 81 119 38 329.78 8.63 255 82 85 3 854.48 0.94 256 82 86 4 844.42 1.98 257 83 86 3 855.93 0.21 258 83 87 4 839.58 4.40 259 83 88 5 589.49 61.70 260 83 121 38 427.30 15.93 261 84 87 3 852.74 1.78 262 84 119 35 438.08 20.54 263 84 120 36 438.07 20.54 264 84 121 37 427.36 15.96 265 88 121 33 499.94 19.22 266 89 121 32 499.94 19.22 267 89 122 33 499.93 19.22 268 90 122 32 499.93 19.21 269 90 124 34 499.92 19.21 270 90 161 71 499.93 19.21 271 91 116 25 500.22 19.27 272 91 121 30 500.22 19.27 273 91 122 31 500.08 19.21 274 91 123 32 499.94 19.21 275 91 124 33 499.94 19.21 276 92 95 3 911.84 2.77 277 92 124 32 499.94 19.21 278 92 126 34 499.93 19.22 279 92 230 138 206.01 0.06 280 93 109 16 500.28 19.28 281 93 112 19 500.34 19.27 282 93 113 20 500.27 19.27 283 93 116 23 500.37 19.28 284 93 123 30 500.20 19.26 285 93 124 31 500.01 19.23 286 93 125 32 499.94 19.21 287 93 126 33 499.93 19.22 288 94 99 5 655.66 83.81 289 94 109 15 500.29 19.28 290 94 126 32 499.93 19.22 291 95 98 3 849.93 0.42 292 95 99 4 830.37 1.73 293 95 126 31 499.94 19.23 294 95 165 70 499.91 19.24 295 95 230 135 206.11 0.10 296 96 99 3 833.55 3.31 297 96 100 4 830.37 1.73 298 96 165 69 499.90 19.24 299 96 167 71 499.76 19.23 300 96 170 74 457.04 18.03 301 97 100 3 832.51 2.79 302 97 101 4 808.75 7.29 303 98 102 4 655.58 83.77 304 98 103 5 645.69 78.82 305 98 104 6 501.87 19.57 306 99 103 4 501.12 19.00 307 99 104 5 500.90 19.08 308 99 106 7 501.07 19.16 309 99 109 10 500.90 19.08 310 99 126 27 500.19 19.31 311 99 127 28 500.17 19.31 312 99 146 47 500.34 19.25 313 104 108 4 835.00 3.13 314 104 112 8 751.83 29.64 315 105 108 3 848.60 1.88 316 105 109 4 840.67 0.32 317 106 109 3 851.48 0.43 318 106 110 4 840.66 0.31 319 106 146 40 500.40 19.28 320 106 149 43 500.60 19.20 321 107 110 3 846.95 2.83 322 107 111 4 840.65 0.32 323 108 111 3 844.96 1.86 324 108 112 4 840.69 0.34 325 109 112 3 845.98 2.98 326 109 113 4 842.49 1.24 327 109 125 16 500.44 19.27 328 109 150 41 500.80 19.21 329 110 113 3 846.84 0.92 330 110 114 4 842.07 1.45 331 110 149 39 500.80 19.21 332 110 150 40 500.79 19.21 333 110 152 42 501.51 19.23 334 110 153 43 507.31 16.32 335 111 114 3 847.44 2.25 336 111 115 4 842.32 1.35 337 112 115 3 859.39 2.60 338 112 116 4 839.05 7.58 339 113 116 3 849.57 2.31 340 113 117 4 842.26 5.96 341 113 123 10 501.55 19.55 342 113 125 12 500.84 19.23 343 113 150 37 501.10 19.12 344 113 153 40 508.09 16.31 345 113 154 41 509.54 16.95 346 113 160 47 507.60 16.54 347 114 117 3 867.28 4.75 348 114 118 4 839.50 0.07 349 114 153 39 507.66 16.48 350 115 118 3 854.98 1.41 351 115 119 4 850.91 1.63 352 116 119 3 861.40 0.97 353 116 120 4 838.06 4.79 354 116 121 5 671.95 5.08 355 117 120 3 833.91 2.71 356 117 121 4 816.08 5.32 357 117 153 36 615.46 31.89 358 117 154 37 615.46 31.89 359 117 160 43 612.58 33.30 360 122 159 37 721.53 30.36 361 123 159 36 721.66 30.32 362 123 160 37 721.64 30.32 363 123 161 38 721.62 30.33 364 124 161 37 721.64 30.32 365 124 163 39 721.62 30.33 366 124 207 83 291.41 0.07 367 124 230 106 206.01 0.06 368 125 150 25 722.01 30.34 369 125 160 35 721.76 30.32 370 125 161 36 721.68 30.32 371 125 162 37 721.65 30.32 372 125 163 38 721.64 30.32 373 126 163 37 721.65 30.32 374 126 165 39 721.61 30.32 375 126 230 104 206.11 0.03 376 127 143 16 722.37 30.36 377 127 146 19 722.27 30.34 378 127 147 20 722.21 30.37 379 127 150 23 722.24 30.37 380 127 162 35 721.75 30.32 381 127 163 36 721.68 30.32 382 127 164 37 721.65 30.32 383 127 165 38 721.64 30.32 384 127 188 61 301.40 2.07 385 128 143 15 722.37 30.36 386 128 146 18 722.36 30.36 387 128 164 36 721.66 30.32 388 128 165 37 721.65 30.32 389 128 167 39 721.51 30.30 390 129 133 4 832.33 2.40 391 129 134 5 825.07 1.24 392 129 139 10 722.40 30.36 393 129 142 13 722.39 30.36 394 129 164 35 721.72 30.30 395 129 167 38 721.61 30.32 396 129 168 39 721.51 30.30 397 130 133 3 839.05 5.75 398 130 134 4 832.38 2.40 399 130 167 37 721.51 30.30 400 130 168 38 721.50 30.30 401 131 134 3 857.58 0.83 402 131 135 4 832.40 2.43 403 131 168 37 721.50 30.31 404 131 172 41 673.18 26.19 405 131 174 43 643.66 29.71 406 132 135 3 848.63 1.71 407 132 136 4 832.40 2.43 408 133 136 3 850.38 5.11 409 133 137 4 832.34 2.42 410 133 138 5 723.32 30.67 411 133 142 9 722.58 30.32 412 134 137 3 831.18 2.98 413 134 139 5 722.61 30.32 414 134 164 30 721.88 30.32 415 134 168 34 721.63 30.32 416 134 184 50 302.15 2.11 417 138 141 3 855.10 2.73 418 138 142 4 844.99 7.78 419 139 142 3 855.10 2.73 420 139 143 4 844.99 7.78 421 139 184 45 301.86 2.10 422 140 143 3 861.75 0.61 423 140 144 4 846.04 8.47 424 140 187 47 301.62 2.14 425 141 144 3 859.85 2.87 426 141 145 4 846.01 8.46 427 142 145 3 864.01 0.79 428 142 146 4 846.37 8.30 429 143 146 3 862.70 0.14 430 143 147 4 848.08 7.44 431 143 164 21 721.77 30.32 432 143 184 41 301.74 2.16 433 143 188 45 301.43 2.08 434 143 191 48 301.36 2.11 435 144 147 3 852.22 5.41 436 144 148 4 851.83 5.57 437 144 191 47 301.18 2.19 438 145 148 3 854.03 4.46 439 145 149 4 854.03 4.46 440 146 149 3 862.94 0.05 441 146 150 4 862.96 0.06 442 147 150 3 861.83 0.62 443 147 151 4 847.80 1.34 444 147 191 44 301.12 2.21 445 147 192 45 301.11 2.20 446 148 151 3 861.82 0.61 447 148 195 47 299.85 2.27 448 150 153 3 868.88 0.18 449 150 154 4 861.44 1.65 450 150 162 12 721.80 30.30 451 151 154 3 864.04 0.78 452 151 192 41 300.58 2.18 453 151 195 44 299.30 2.06 454 151 196 45 298.94 1.93 455 154 157 3 884.63 0.92 456 154 160 6 721.84 30.28 457 154 204 50 298.23 2.04 458 156 159 3 859.40 0.42 459 157 192 35 299.89 1.85 460 157 196 39 298.86 1.88 461 157 200 43 298.53 1.91 462 157 203 46 298.26 2.02 463 157 204 47 298.17 2.04 464 158 161 3 922.96 1.55 465 158 203 45 298.27 2.04 466 158 205 47 298.16 2.05 467 160 204 44 298.08 2.02 468 161 205 44 296.24 2.00 469 161 207 46 291.43 0.07 470 162 188 26 300.87 2.31 471 162 192 30 300.62 2.19 472 162 204 42 298.15 2.05 473 162 205 43 297.94 1.96 474 162 206 44 291.45 0.06 475 162 207 45 291.45 0.06 476 163 207 44 291.39 0.06 477 163 209 46 290.56 0.26 478 163 230 67 206.11 0.03 479 164 184 20 301.41 2.07 480 164 185 21 301.40 2.06 481 164 188 24 301.38 2.08 482 164 207 43 291.46 0.05 483 164 208 44 291.36 0.07 484 164 209 45 291.07 0.04 485 165 170 5 840.30 2.83 486 165 209 44 291.05 0.04 487 166 169 3 874.81 5.80 488 166 170 4 840.30 2.83 489 166 176 10 302.38 2.12 490 166 208 42 291.78 0.21 491 166 209 43 291.35 0.07 492 166 210 44 290.28 0.04 493 167 170 3 851.84 2.94 494 167 172 5 825.93 10.01 495 168 172 4 830.39 3.59 496 168 174 6 675.17 5.08 497 168 176 8 342.34 4.89 498 168 180 12 316.05 4.70 499 169 172 3 850.58 2.17 500 169 174 5 535.98 74.03 501 169 175 6 377.38 2.68 502 169 176 7 330.88 0.84 503 176 180 4 834.48 2.21 504 176 208 32 297.18 2.09 505 177 180 3 853.57 0.54 506 177 181 4 846.76 3.94 507 178 181 3 853.10 0.77 508 178 182 4 853.59 0.52 509 178 219 41 220.52 2.93 510 178 220 42 218.12 1.75 511 178 223 45 214.77 0.27 512 179 182 3 853.61 0.51 513 179 183 4 853.60 0.51 514 180 183 3 859.71 2.54 515 180 184 4 853.61 0.51 516 181 184 3 862.78 1.43 517 181 185 4 845.10 3.73 518 181 208 27 297.44 2.21 519 181 220 39 218.10 1.75 520 181 226 45 213.18 0.21 521 182 185 3 853.42 0.94 522 182 186 4 844.59 3.48 523 182 223 41 213.30 0.19 524 182 224 42 213.30 0.19 525 182 225 43 213.19 0.20 526 182 226 44 213.13 0.23 527 183 186 3 853.35 0.91 528 183 187 4 846.58 2.48 529 184 187 3 846.64 2.52 530 184 188 4 844.22 1.30 531 185 188 3 853.14 0.72 532 185 189 4 855.39 2.23 533 185 206 21 298.18 1.98 534 185 207 22 298.06 2.02 535 185 208 23 297.93 2.06 536 185 225 40 212.73 0.17 537 185 226 41 212.02 0.07 538 186 189 3 857.12 1.36 539 186 190 4 857.12 1.36 540 186 225 39 212.97 0.29 541 187 190 3 857.13 1.36 542 187 191 4 855.39 2.23 543 188 191 3 855.39 2.23 544 188 192 4 855.38 2.23 545 188 206 18 298.16 2.05 546 189 192 3 847.21 2.08 547 189 193 4 839.95 1.18 548 189 201 12 298.79 1.89 549 189 202 13 298.78 1.89 550 189 204 15 298.66 1.90 551 189 206 17 298.24 2.02 552 189 225 36 212.17 0.03 553 189 227 38 212.05 0.08 554 190 193 3 848.66 3.11 555 190 194 4 834.46 1.55 556 191 194 3 857.85 1.48 557 191 195 4 829.33 5.75 558 192 195 3 851.32 4.18 559 192 196 4 708.95 4.30 560 192 197 5 708.64 4.46 561 192 200 8 345.18 7.38 562 192 201 9 298.77 1.89 563 192 204 12 298.44 2.00 564 193 197 4 374.38 6.73 565 193 198 5 335.18 10.13 566 193 201 8 298.82 1.89 567 196 200 4 821.85 1.57 568 197 200 3 852.50 0.76 569 197 201 4 834.85 8.06 570 198 201 3 855.43 2.93 571 198 202 4 838.61 6.19 572 199 202 3 852.51 0.77 573 199 203 4 844.64 3.16 574 200 203 3 852.50 0.77 575 200 204 4 839.56 0.62 576 201 204 3 852.96 0.55 577 204 227 23 210.27 0.61 578 205 227 22 210.11 0.55 579 206 225 19 210.97 0.38 580 206 226 20 210.38 0.62 581 206 227 21 210.16 0.57 582 207 227 20 210.11 0.56 583 207 228 21 207.36 0.35 584 207 229 22 207.29 0.37 585 207 230 23 207.31 0.36 586 208 212 4 866.46 2.57 587 208 220 12 213.88 0.43 588 208 226 18 210.44 0.65 589 208 228 20 209.47 0.48 590 208 229 21 207.29 0.37 591 208 230 22 207.31 0.36 592 209 230 21 206.70 0.16 593 211 216 5 808.25 2.27 594 212 216 4 815.53 5.92 595 212 217 5 589.74 102.14 596 212 220 8 348.95 7.91 597 212 229 17 207.39 0.35 598 212 231 19 207.25 0.29 599 212 234 22 206.47 0.24 600 212 243 31 51.08 0.54 601 213 216 3 856.36 0.79 602 213 217 4 808.37 2.22 603 213 234 21 206.62 0.16 604 213 243 30 51.13 0.53 605 214 217 3 863.34 0.87 606 214 239 25 190.74 0.76 607 214 242 28 51.19 0.52 608 214 243 29 51.09 0.57 609 216 219 3 868.58 3.49 610 216 220 4 834.37 1.72 611 217 220 3 851.27 0.67 612 217 221 4 834.49 1.78 613 217 229 12 212.68 0.21 614 217 243 26 45.40 0.17 615 217 246 29 36.12 0.71 616 218 221 3 877.80 3.91 617 218 222 4 834.48 1.78 618 218 246 28 37.24 0.15 619 219 222 3 877.80 3.90 620 219 223 4 845.31 1.23 621 220 223 3 872.52 3.10 622 220 226 6 410.66 45.49 623 220 229 9 213.16 0.21 624 221 226 5 308.91 0.61 625 221 227 6 307.30 0.18 626 221 228 7 213.25 0.24 627 221 229 8 213.25 0.24 628 221 246 25 35.94 0.78 629 221 247 26 29.98 1.88 630 223 226 3 840.92 1.25 631 229 243 14 44.42 0.06 632 229 246 17 35.96 0.79 633 231 234 3 876.49 1.66 634 231 235 4 818.53 0.84 635 231 240 9 51.27 0.48 636 231 243 12 50.12 0.27 637 232 235 3 870.57 4.62 638 233 236 3 864.07 0.18 639 233 237 4 862.70 5.21 640 234 237 3 867.22 2.94 641 234 240 6 342.50 14.44 642 234 243 9 185.57 66.67 643 235 240 5 350.85 10.27 644 237 240 3 866.19 0.11 645 238 241 3 873.51 0.94 646 239 242 3 857.34 2.06 647 239 243 4 859.59 3.19 648 240 243 3 859.59 3.19 649 240 244 4 844.79 0.76 650 241 244 3 849.84 3.29 651 241 245 4 839.91 1.68 652 242 245 3 844.55 0.65 653 242 246 4 832.89 1.84 654 243 246 3 850.93 2.54 655 243 247 4 849.65 1.28 656 244 247 3 865.32 2.44 657 244 248 4 844.93 3.65 658 245 248 3 846.83 4.60