BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 9 8 780.85 19.37 2 1 34 33 42.27 0.03 3 1 35 34 29.75 0.33 4 1 234 233 37.95 0.33 5 1 236 235 29.75 0.34 6 2 5 3 835.50 3.83 7 2 6 4 834.38 4.38 8 2 33 31 43.03 0.08 9 3 6 3 844.98 2.48 10 3 7 4 851.17 4.00 11 3 27 24 43.80 0.42 12 3 30 27 43.09 0.07 13 3 31 28 43.09 0.07 14 3 32 29 43.73 0.39 15 3 33 30 43.05 0.07 16 3 35 32 42.80 0.14 17 3 92 89 43.03 0.07 18 3 94 91 43.06 0.06 19 4 7 3 851.17 4.01 20 4 8 4 851.16 4.01 21 5 8 3 851.16 4.01 22 5 9 4 834.28 4.42 23 6 9 3 832.34 3.46 24 6 10 4 826.11 0.34 25 6 35 29 52.33 0.91 26 6 38 32 52.32 0.91 27 6 61 55 52.33 0.91 28 6 94 88 52.34 0.91 29 6 230 224 52.32 0.92 30 6 236 230 52.33 0.91 31 7 10 3 845.16 0.84 32 7 11 4 834.27 3.75 33 7 26 19 52.34 0.91 34 7 27 20 52.33 0.91 35 7 94 87 52.33 0.91 36 7 96 89 53.35 0.41 37 8 11 3 837.80 1.98 38 8 12 4 834.26 3.74 39 9 12 3 844.52 3.71 40 9 13 4 831.06 5.34 41 9 229 220 58.84 0.29 42 9 230 221 58.82 0.29 43 9 234 225 58.84 0.29 44 10 13 3 839.46 6.24 45 10 14 4 821.80 0.72 46 10 61 51 58.76 0.32 47 10 96 86 58.99 0.38 48 10 226 216 58.82 0.29 49 10 230 220 58.87 0.30 50 11 14 3 842.77 0.52 51 11 15 4 781.47 19.00 52 11 16 5 625.90 23.74 53 11 96 85 59.18 0.46 54 12 15 3 821.36 0.94 55 13 103 90 64.81 1.83 56 13 105 92 64.80 1.83 57 13 106 93 64.76 1.81 58 13 229 216 64.58 1.79 59 14 96 82 64.78 1.83 60 14 99 85 64.79 1.83 61 14 100 86 64.78 1.83 62 14 103 89 64.81 1.83 63 14 226 212 64.66 1.77 64 15 18 3 863.05 0.24 65 15 19 4 845.10 0.65 66 15 103 88 65.05 1.80 67 16 19 3 853.18 4.69 68 16 25 9 65.00 1.79 69 16 96 80 64.99 1.78 70 16 99 83 65.00 1.78 71 19 98 79 96.28 1.34 72 19 99 80 77.83 4.16 73 20 98 78 113.14 6.11 74 21 25 4 834.44 3.21 75 21 69 48 251.94 30.98 76 21 97 76 115.73 4.86 77 21 98 77 115.70 4.83 78 21 99 78 115.77 4.80 79 22 68 46 251.92 30.98 80 22 69 47 251.93 30.98 81 22 97 75 170.43 6.33 82 23 66 43 251.91 30.96 83 23 67 44 251.91 30.97 84 23 68 45 251.94 30.97 85 23 69 46 251.95 30.98 86 24 28 4 777.01 26.30 87 24 66 42 252.03 30.92 88 24 67 43 252.04 30.93 89 25 95 70 250.15 29.98 90 25 96 71 249.92 29.96 91 25 97 72 249.92 29.96 92 25 99 74 236.66 23.36 93 26 94 68 252.05 30.91 94 26 95 69 252.03 30.90 95 26 96 70 250.19 29.98 96 27 30 3 884.59 4.74 97 27 92 65 254.20 30.64 98 27 93 66 254.12 30.61 99 27 94 67 253.20 30.34 100 28 65 37 254.12 30.61 101 28 66 38 254.12 30.61 102 28 67 39 254.23 30.62 103 28 93 65 254.20 30.64 104 28 94 66 254.12 30.61 105 28 95 67 254.09 30.59 106 29 67 38 254.62 30.67 107 29 83 54 254.69 30.64 108 29 86 57 255.31 30.47 109 29 87 58 255.59 30.46 110 29 92 63 254.60 30.68 111 29 93 64 254.38 30.69 112 30 87 57 255.62 30.46 113 30 92 62 254.67 30.65 114 31 90 59 255.63 30.48 115 31 91 60 255.61 30.48 116 31 92 61 255.41 30.53 117 32 58 26 489.20 84.81 118 32 90 58 265.06 27.24 119 32 92 60 255.69 30.44 120 34 58 24 755.88 18.29 121 35 38 3 933.07 2.58 122 35 58 23 756.60 18.64 123 35 59 24 748.28 18.83 124 35 92 57 404.44 1.58 125 35 236 201 120.56 0.04 126 36 58 22 766.33 22.21 127 36 235 199 120.62 0.07 128 36 236 200 120.52 0.03 129 36 237 201 120.50 0.02 130 37 55 18 766.59 22.16 131 37 56 19 766.57 22.16 132 37 57 20 766.35 22.21 133 37 58 21 766.34 22.21 134 37 59 22 766.33 22.20 135 37 237 200 120.41 0.00 136 38 59 21 766.33 22.21 137 38 61 23 766.30 22.20 138 38 236 198 120.56 0.03 139 38 237 199 120.31 0.01 140 38 238 200 118.20 0.40 141 38 239 201 118.20 0.40 142 39 48 9 766.72 22.14 143 39 52 13 766.61 22.16 144 39 55 16 766.62 22.16 145 39 57 18 766.57 22.17 146 39 59 20 766.50 22.19 147 39 60 21 766.32 22.21 148 39 61 22 766.32 22.21 149 39 239 200 118.17 0.41 150 39 241 202 113.67 0.89 151 40 48 8 766.76 22.14 152 40 61 21 766.31 22.20 153 40 223 183 173.31 0.66 154 40 227 187 160.90 1.41 155 40 238 198 118.20 0.40 156 40 239 199 118.12 0.38 157 40 240 200 113.67 0.88 158 40 241 201 113.67 0.88 159 41 48 7 766.77 22.14 160 41 61 20 766.52 22.18 161 41 62 21 766.30 22.20 162 41 63 22 766.34 22.21 163 41 223 182 173.25 0.67 164 41 240 199 113.68 0.89 165 41 241 200 113.25 0.92 166 42 62 20 766.47 22.18 167 42 63 21 766.34 22.21 168 42 64 22 766.33 22.21 169 42 216 174 191.74 1.54 170 42 219 177 191.55 1.61 171 42 220 178 186.40 0.34 172 42 223 181 173.22 0.68 173 43 48 5 783.70 16.65 174 43 62 19 766.52 22.18 175 43 64 21 766.31 22.20 176 44 48 4 783.73 16.64 177 44 49 5 783.67 16.64 178 44 62 18 766.55 22.18 179 44 64 20 766.38 22.19 180 44 66 22 766.13 22.18 181 44 82 38 418.77 0.18 182 44 93 49 415.27 0.11 183 45 48 3 839.73 0.36 184 45 49 4 790.84 20.18 185 45 82 37 418.78 0.18 186 46 49 3 840.19 0.59 187 46 50 4 830.10 0.56 188 46 81 35 418.75 0.14 189 46 82 36 418.75 0.17 190 46 85 39 418.65 0.14 191 47 50 3 850.16 0.36 192 47 51 4 830.09 0.57 193 47 243 196 107.84 0.68 194 48 51 3 842.54 3.33 195 48 52 4 830.08 0.56 196 48 60 12 766.62 22.16 197 48 62 14 766.52 22.18 198 48 241 193 113.58 0.91 199 48 243 195 107.84 0.68 200 49 52 3 830.11 0.56 201 49 53 4 840.27 4.55 202 49 60 11 766.63 22.16 203 49 66 17 766.35 22.18 204 49 82 33 418.60 0.09 205 49 85 36 418.48 0.07 206 49 86 37 418.46 0.06 207 50 53 3 848.06 0.66 208 50 54 4 840.30 4.54 209 50 85 35 418.54 0.07 210 51 54 3 849.47 0.03 211 51 55 4 840.31 4.54 212 51 241 190 114.30 1.20 213 51 243 192 113.64 0.88 214 52 55 3 849.47 0.04 215 52 56 4 784.14 16.31 216 52 57 5 766.91 22.13 217 52 60 8 766.60 22.14 218 52 91 39 416.26 0.01 219 53 56 3 841.72 3.83 220 53 85 32 418.50 0.05 221 53 89 36 416.55 0.12 222 53 91 38 416.28 0.01 223 55 241 186 118.52 0.24 224 57 91 34 416.25 0.01 225 58 91 33 416.20 0.01 226 58 92 34 416.19 0.01 227 59 91 32 416.19 0.01 228 59 92 33 415.88 0.06 229 59 94 35 415.83 0.06 230 60 91 31 416.19 0.01 231 60 92 32 415.88 0.06 232 60 93 33 415.39 0.05 233 60 94 34 415.28 0.10 234 61 94 33 414.42 0.32 235 61 96 35 414.32 0.36 236 61 223 162 173.19 0.69 237 61 226 165 160.96 1.41 238 61 230 169 142.07 5.42 239 62 65 3 866.99 1.79 240 62 93 31 415.39 0.05 241 62 94 32 414.87 0.10 242 62 95 33 413.97 0.26 243 62 96 34 413.96 0.27 244 62 223 161 173.00 0.61 245 63 96 33 413.95 0.26 246 63 97 34 413.94 0.27 247 63 100 37 380.45 0.60 248 63 101 38 380.47 0.61 249 63 108 45 193.12 1.18 250 63 219 156 191.49 1.66 251 64 68 4 895.21 1.11 252 64 97 33 413.97 0.26 253 64 215 151 192.64 1.85 254 64 219 155 192.09 1.84 255 65 68 3 1041.21 0.58 256 66 82 16 418.76 0.21 257 66 83 17 418.76 0.21 258 66 86 20 418.75 0.21 259 66 93 27 415.69 0.00 260 67 71 4 792.20 27.82 261 67 79 12 418.83 0.17 262 68 71 3 854.18 3.17 263 68 97 29 415.00 0.08 264 71 79 8 549.89 61.14 265 74 79 5 739.08 0.22 266 75 79 4 739.08 0.21 267 75 80 5 734.89 2.28 268 75 83 8 638.19 35.45 269 76 79 3 847.38 0.46 270 76 80 4 739.00 0.25 271 77 80 3 850.87 0.42 272 77 81 4 834.02 8.01 273 78 81 3 850.88 0.42 274 78 82 4 819.86 1.38 275 79 82 3 836.65 6.69 276 79 83 4 824.24 0.86 277 80 83 3 848.06 0.57 278 80 84 4 828.19 1.10 279 81 84 3 850.16 1.62 280 81 85 4 833.08 3.54 281 82 85 3 850.04 1.70 282 82 86 4 850.24 1.59 283 83 86 3 850.25 1.59 284 83 87 4 829.12 5.53 285 84 87 3 855.09 1.29 286 84 88 4 789.52 16.62 287 85 89 4 830.36 5.80 288 86 89 3 847.43 2.73 289 86 90 4 742.51 3.90 290 86 91 5 420.95 2.40 291 86 93 7 415.91 0.07 292 87 90 3 844.43 1.24 293 96 99 3 865.43 0.82 294 96 100 4 788.81 26.71 295 97 100 3 862.66 0.56 296 97 101 4 832.72 4.76 297 98 101 3 833.41 4.42 298 98 102 4 807.05 17.61 299 98 127 29 198.47 3.72 300 99 102 3 844.18 0.97 301 99 103 4 788.77 26.73 302 100 106 6 206.83 5.98 303 100 107 7 197.95 3.56 304 100 108 8 197.93 3.56 305 100 222 122 187.86 6.62 306 100 223 123 184.39 4.90 307 100 226 126 167.34 1.81 308 101 107 6 197.98 3.56 309 101 108 7 197.92 3.54 310 101 109 8 197.97 3.57 311 101 127 26 199.16 3.40 312 101 172 71 197.91 3.54 313 101 219 118 197.69 3.58 314 102 127 25 199.33 3.48 315 103 106 3 831.72 3.65 316 103 107 4 307.82 56.77 317 103 110 7 333.80 43.79 318 104 110 6 427.45 1.57 319 105 110 5 653.35 40.36 320 105 111 6 653.43 40.30 321 106 111 5 653.47 40.32 322 106 222 116 194.49 3.37 323 106 225 119 184.11 4.87 324 106 226 120 170.91 0.37 325 106 229 123 157.63 0.40 326 107 110 3 835.12 4.91 327 107 111 4 827.04 4.62 328 107 222 115 187.91 6.65 329 108 111 3 840.33 2.02 330 108 112 4 830.82 2.74 331 108 172 64 278.88 12.70 332 108 218 110 199.88 3.77 333 108 219 111 199.64 3.68 334 108 222 114 187.91 6.64 335 109 112 3 833.19 3.04 336 109 113 4 830.83 2.74 337 109 127 18 282.33 13.44 338 109 129 20 282.36 13.43 339 109 170 61 281.31 13.51 340 109 171 62 279.28 12.87 341 109 172 63 278.91 12.70 342 110 113 3 841.81 1.27 343 110 114 4 832.31 3.48 344 111 114 3 836.33 1.47 345 111 115 4 829.41 4.92 346 111 221 110 197.50 3.68 347 111 222 111 197.25 3.80 348 111 225 114 192.98 4.69 349 112 115 3 833.36 2.96 350 112 116 4 791.01 14.28 351 112 150 38 283.87 13.52 352 112 170 58 282.89 13.50 353 112 172 60 282.57 13.34 354 112 191 79 282.57 13.34 355 112 218 106 217.00 12.31 356 113 116 3 842.84 2.10 357 113 117 4 785.49 16.35 358 113 118 5 476.76 109.92 359 113 120 7 283.95 13.52 360 113 170 57 283.36 13.34 361 114 117 3 842.07 3.42 362 115 149 34 283.92 13.54 363 115 150 35 283.92 13.54 364 116 133 17 296.51 14.57 365 116 145 29 283.97 13.53 366 116 146 30 283.93 13.53 367 116 149 33 283.94 13.53 368 116 191 75 283.95 13.52 369 117 133 16 296.51 14.56 370 117 134 17 296.53 14.58 371 118 131 13 297.63 14.65 372 118 132 14 297.58 14.70 373 118 133 15 296.53 14.56 374 118 168 50 297.29 14.68 375 118 170 52 296.38 14.56 376 118 191 73 296.48 14.60 377 119 131 12 299.27 13.88 378 119 132 13 298.28 14.37 379 119 134 15 297.97 14.52 380 120 129 9 313.86 7.12 381 120 130 10 299.87 14.12 382 120 131 11 299.81 14.13 383 120 170 50 299.81 14.14 384 121 130 9 314.95 6.99 385 121 131 10 311.58 8.68 386 121 132 11 314.79 7.08 387 122 129 7 318.49 5.44 388 122 130 8 317.66 5.78 389 123 126 3 879.73 1.98 390 123 128 5 318.83 5.38 391 123 129 6 318.45 5.42 392 123 130 7 318.58 5.36 393 124 128 4 615.74 21.38 394 124 130 6 319.51 5.72 395 124 169 45 319.49 5.71 396 124 181 57 321.81 6.69 397 124 182 58 321.47 6.69 398 124 185 61 321.84 6.67 399 124 187 63 321.81 6.69 400 125 128 3 821.16 1.36 401 125 178 53 321.50 6.58 402 125 181 56 321.92 6.63 403 127 171 44 320.25 6.06 404 127 178 51 321.93 6.63 405 128 169 41 322.15 6.54 406 128 170 42 321.85 6.67 407 128 171 43 321.00 6.32 408 128 178 50 321.93 6.63 409 129 169 40 322.21 6.55 410 129 170 41 322.08 6.56 411 130 167 37 323.25 6.39 412 130 168 38 322.26 6.55 413 130 169 39 322.18 6.54 414 130 170 40 322.18 6.54 415 130 185 55 323.26 6.33 416 131 167 36 323.35 6.36 417 131 168 37 323.10 6.38 418 131 170 39 323.10 6.37 419 132 166 34 323.54 6.27 420 132 167 35 323.35 6.36 421 133 136 3 845.71 0.43 422 133 137 4 847.65 0.54 423 133 142 9 329.30 9.33 424 133 165 32 453.69 2.67 425 133 166 33 398.35 24.99 426 133 168 35 323.36 6.35 427 136 164 28 543.47 0.86 428 136 165 29 542.84 0.77 429 136 166 30 533.15 0.24 430 137 141 4 783.20 16.23 431 137 142 5 700.55 16.25 432 137 164 27 543.50 0.85 433 137 165 28 543.35 0.82 434 138 141 3 845.47 6.21 435 138 142 4 826.81 6.42 436 138 163 25 543.60 0.83 437 138 164 26 543.59 0.83 438 139 142 3 842.20 1.27 439 139 143 4 842.20 1.27 440 139 156 17 543.40 0.85 441 139 165 26 544.73 0.28 442 139 209 70 543.40 0.85 443 139 211 72 543.40 0.85 444 140 143 3 847.52 1.39 445 140 144 4 798.68 23.03 446 140 156 16 544.65 0.24 447 141 144 3 847.52 1.39 448 141 145 4 789.96 18.66 449 142 145 3 834.35 5.19 450 142 146 4 792.76 17.27 451 142 165 23 545.45 0.09 452 142 189 47 545.42 0.08 453 142 211 69 545.24 0.07 454 143 146 3 841.75 1.49 455 143 147 4 792.77 17.26 456 143 154 11 545.41 0.09 457 143 156 13 545.40 0.09 458 143 211 68 545.23 0.07 459 144 147 3 826.76 0.26 460 144 148 4 793.12 17.05 461 145 148 3 826.72 0.24 462 145 149 4 824.70 1.24 463 146 149 3 835.83 4.33 464 146 150 4 749.45 3.01 465 146 154 8 545.68 0.12 466 146 191 45 545.55 0.13 467 146 211 65 545.60 0.15 468 146 213 67 545.20 0.07 469 147 150 3 841.78 1.35 470 147 151 4 700.16 21.18 471 147 152 5 545.74 0.10 472 147 153 6 545.69 0.12 473 147 154 7 545.69 0.12 474 150 213 63 545.54 0.13 475 150 217 67 527.53 0.53 476 150 218 68 527.53 0.53 477 150 220 70 527.24 0.52 478 152 212 60 545.96 0.30 479 152 213 61 545.58 0.14 480 152 214 62 545.54 0.14 481 152 217 65 527.53 0.53 482 152 246 94 64.02 0.04 483 154 211 57 545.97 0.32 484 154 212 58 545.94 0.32 485 154 213 59 545.74 0.24 486 155 195 40 546.11 0.26 487 155 200 45 547.02 0.61 488 155 210 55 546.83 0.59 489 155 211 56 546.12 0.25 490 155 212 57 545.98 0.32 491 156 209 53 547.02 0.61 492 156 210 54 546.83 0.59 493 156 211 55 546.19 0.28 494 157 200 43 547.03 0.61 495 157 203 46 547.06 0.60 496 157 204 47 548.71 0.62 497 157 208 51 547.25 0.65 498 157 209 52 547.01 0.60 499 157 210 53 546.99 0.60 500 158 161 3 755.11 4.05 501 158 209 51 547.01 0.60 502 159 204 45 549.95 0.56 503 160 203 43 549.96 0.56 504 160 204 44 549.96 0.56 505 160 207 47 550.07 0.50 506 161 207 46 550.12 0.50 507 161 208 47 550.05 0.49 508 161 209 48 549.94 0.55 509 162 188 26 580.90 13.77 510 162 207 45 592.00 8.23 511 162 208 46 591.46 8.50 512 162 209 47 562.17 2.02 513 165 168 3 914.28 0.99 514 165 188 23 639.25 2.73 515 165 189 24 634.65 0.43 516 165 209 44 631.12 1.33 517 166 188 22 651.25 1.69 518 167 185 18 651.92 1.43 519 167 186 19 651.86 1.43 520 167 187 20 651.62 1.53 521 167 188 21 651.41 1.61 522 167 189 22 651.41 1.61 523 168 187 19 651.75 1.47 524 168 189 21 651.55 1.55 525 168 191 23 651.87 1.41 526 169 182 13 651.89 1.42 527 169 187 18 651.88 1.42 528 169 189 20 651.69 1.52 529 169 190 21 651.63 1.53 530 169 191 22 651.90 1.40 531 170 190 20 651.89 1.41 532 170 191 21 651.90 1.41 533 171 175 4 653.29 0.72 534 171 178 7 652.04 1.36 535 171 190 19 652.04 1.36 536 171 191 20 652.84 0.95 537 171 192 21 652.01 1.34 538 172 218 46 602.58 2.75 539 172 219 47 602.48 2.77 540 173 192 19 652.99 0.89 541 173 193 20 652.94 0.86 542 173 194 21 652.96 0.88 543 173 215 42 639.39 2.32 544 173 218 45 603.28 2.44 545 173 219 46 603.14 2.48 546 174 194 20 652.97 0.87 547 174 215 41 649.52 1.78 548 175 178 3 852.85 1.01 549 175 190 15 653.38 0.86 550 175 192 17 653.11 0.84 551 176 179 3 839.70 2.94 552 176 190 14 653.45 0.86 553 176 192 16 653.17 0.81 554 176 194 18 653.25 0.87 555 176 195 19 653.22 0.90 556 176 196 20 653.28 0.93 557 176 199 23 653.20 0.97 558 178 181 3 849.10 3.29 559 178 182 4 781.66 17.36 560 178 190 12 653.53 0.89 561 179 182 3 831.82 3.60 562 179 183 4 787.63 20.34 563 179 190 11 653.50 0.88 564 179 199 20 653.36 0.95 565 179 202 23 653.38 1.03 566 179 203 24 653.38 1.03 567 179 208 29 652.91 1.28 568 179 210 31 652.73 1.26 569 180 183 3 794.97 16.67 570 180 184 4 757.43 5.24 571 180 202 22 653.37 1.02 572 180 206 26 653.22 1.19 573 181 184 3 834.99 0.70 574 181 185 4 834.98 0.71 575 182 185 3 834.99 0.71 576 182 186 4 757.38 5.26 577 182 187 5 706.22 20.05 578 182 190 8 653.56 0.91 579 183 186 3 822.14 1.19 580 183 206 23 693.79 21.54 581 183 208 25 658.23 3.93 582 187 208 21 731.50 40.55 583 188 208 20 731.48 40.56 584 188 209 21 731.47 40.58 585 189 209 20 731.45 40.58 586 189 211 22 731.45 40.58 587 190 208 18 731.49 40.59 588 190 209 19 731.47 40.59 589 190 210 20 731.41 40.60 590 190 211 21 731.41 40.60 591 191 211 20 731.38 40.62 592 191 213 22 730.63 40.98 593 192 210 18 731.43 40.62 594 192 211 19 731.40 40.62 595 192 212 20 730.64 40.98 596 192 213 21 730.63 40.98 597 192 218 26 636.88 14.39 598 193 212 19 730.68 40.98 599 193 213 20 730.60 41.00 600 193 214 21 729.27 41.35 601 193 215 22 709.10 40.16 602 193 218 25 636.93 14.37 603 194 210 16 731.50 40.63 604 194 212 18 730.68 40.98 605 194 215 21 730.10 41.23 606 195 199 4 839.17 1.86 607 195 200 5 830.41 1.54 608 195 210 15 731.49 40.63 609 195 212 17 730.69 40.98 610 196 199 3 848.84 2.96 611 196 200 4 848.85 2.96 612 197 200 3 860.00 2.29 613 197 201 4 848.85 2.96 614 198 201 3 850.03 2.37 615 198 202 4 848.84 2.97 616 199 202 3 841.48 6.64 617 199 203 4 831.68 1.96 618 199 210 11 731.53 40.64 619 200 203 3 833.44 1.09 620 200 204 4 829.61 2.69 621 200 210 10 731.54 40.65 622 201 204 3 833.54 0.72 623 201 205 4 828.61 3.16 624 202 205 3 833.41 1.97 625 202 206 4 828.61 3.17 626 203 206 3 854.03 0.27 627 203 207 4 828.62 3.16 628 203 208 5 736.41 38.23 629 203 210 7 731.54 40.64 630 204 207 3 829.85 2.58 631 213 217 4 828.38 1.82 632 213 218 5 779.05 17.25 633 214 217 3 829.31 1.36 634 214 218 4 824.04 0.05 635 214 247 33 19.84 2.26 636 215 218 3 847.29 4.30 637 215 219 4 828.69 0.37 638 215 247 32 18.36 1.55 639 216 219 3 835.51 1.59 640 216 220 4 828.69 0.38 641 216 242 26 105.89 0.15 642 216 243 27 90.44 4.17 643 216 244 28 66.48 1.19 644 216 245 29 45.90 6.08 645 216 247 31 19.38 2.06 646 217 220 3 860.77 2.79 647 219 223 4 845.07 5.65 648 220 223 3 859.21 2.50 649 220 224 4 823.08 3.54 650 220 240 20 114.59 1.31 651 220 242 22 109.59 1.79 652 221 224 3 855.61 0.37 653 221 225 4 824.88 4.45 654 222 225 3 834.69 0.46 655 222 226 4 826.31 3.73 656 223 226 3 842.52 6.17 657 223 227 4 822.87 1.99 658 223 240 17 114.59 1.31 659 224 227 3 846.48 4.19 660 224 228 4 830.19 1.68 661 224 240 16 114.59 1.31 662 225 228 3 844.20 5.33 663 225 229 4 822.89 1.99 664 226 229 3 825.16 3.12 665 226 230 4 822.88 1.99 666 227 230 3 838.90 3.48 667 227 231 4 822.75 2.02 668 227 238 11 119.17 0.03 669 227 240 13 114.65 1.33 670 228 231 3 835.51 0.71 671 228 232 4 787.26 14.61 672 229 232 3 833.55 1.70 673 229 233 4 771.47 22.46 674 230 233 3 835.62 0.66 675 230 234 4 769.60 23.32 676 230 236 6 389.69 134.46 677 230 238 8 120.51 0.02 678 231 234 3 849.62 2.48 679 231 236 5 389.69 134.46 680 231 237 6 120.66 0.01 681 231 238 7 120.56 0.00