BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 52 51 26.28 0.13 2 1 62 61 27.28 0.38 3 2 25 23 25.64 0.28 4 2 49 47 25.95 0.24 5 2 50 48 26.26 0.14 6 3 18 15 33.41 1.25 7 3 21 18 26.60 0.03 8 3 22 19 26.28 0.14 9 3 25 22 26.28 0.14 10 3 50 47 26.34 0.16 11 3 51 48 26.60 0.29 12 3 52 49 32.74 0.42 13 4 52 48 34.59 0.48 14 5 10 5 786.10 5.66 15 5 14 9 50.80 3.54 16 5 15 10 39.90 0.62 17 5 18 13 35.20 0.57 18 5 52 47 35.20 0.57 19 5 53 48 36.41 1.11 20 5 54 49 50.80 3.54 21 5 129 124 59.30 0.92 22 5 132 127 59.25 0.90 23 6 10 4 820.03 3.25 24 6 54 48 56.01 0.94 25 7 10 3 825.70 0.42 26 7 54 47 59.23 0.91 27 7 55 48 59.38 0.83 28 7 56 49 59.50 0.86 29 7 129 122 59.44 0.85 30 7 132 125 59.40 0.83 31 7 133 126 59.43 0.85 32 7 136 129 59.40 0.83 33 8 56 48 59.70 0.96 34 10 14 4 809.93 5.49 35 10 129 119 82.58 0.40 36 11 14 3 845.66 2.85 37 11 15 4 831.36 5.21 38 11 175 164 89.44 1.38 39 12 15 3 832.30 4.74 40 12 16 4 831.39 5.19 41 12 172 160 83.84 1.75 42 12 175 163 93.08 0.25 43 13 16 3 837.22 2.28 44 13 17 4 831.39 5.19 45 14 17 3 837.23 2.29 46 14 18 4 831.86 4.97 47 15 18 3 840.33 0.73 48 15 19 4 824.41 3.94 49 15 129 114 104.64 0.70 50 15 157 142 95.59 0.92 51 15 161 146 94.14 0.26 52 15 168 153 95.03 0.19 53 15 171 156 103.66 0.60 54 15 172 157 104.17 0.65 55 16 19 3 830.85 4.00 56 16 20 4 824.43 3.94 57 16 168 152 95.66 0.51 58 16 172 156 104.59 0.69 59 17 20 3 838.76 2.44 60 17 21 4 824.45 3.93 61 18 21 3 837.97 2.84 62 18 22 4 824.44 3.93 63 18 51 33 109.28 0.72 64 18 53 35 116.25 0.99 65 18 158 140 106.98 1.87 66 19 22 3 831.66 0.97 67 19 23 4 824.44 3.93 68 19 157 138 107.34 1.69 69 19 158 139 107.03 1.84 70 19 161 142 104.58 0.68 71 19 162 143 104.37 0.72 72 19 168 149 105.11 0.93 73 20 23 3 829.67 3.08 74 20 24 4 832.66 1.59 75 21 24 3 841.65 5.16 76 21 25 4 826.40 1.55 77 22 25 3 841.89 5.04 78 22 26 4 838.42 4.34 79 22 49 27 117.80 0.14 80 22 51 29 117.38 0.68 81 22 65 43 117.06 0.72 82 22 158 136 116.67 0.93 83 22 162 140 112.45 0.01 84 23 26 3 848.45 1.77 85 23 161 138 116.93 0.99 86 23 162 139 114.28 0.43 87 23 164 141 116.79 0.99 88 23 165 142 116.88 1.02 89 23 168 145 117.21 0.95 90 23 238 215 109.91 0.48 91 23 239 216 109.84 0.49 92 25 49 24 118.59 0.23 93 26 38 12 117.36 0.74 94 26 49 23 118.65 0.25 95 26 67 41 118.20 0.34 96 26 70 44 117.47 0.77 97 26 239 213 118.34 0.34 98 27 39 12 118.62 0.24 99 27 67 40 118.59 0.23 100 28 36 8 118.69 0.23 101 28 37 9 118.42 0.30 102 28 39 11 118.64 0.24 103 29 39 10 118.72 0.24 104 30 36 6 120.28 0.14 105 30 37 7 119.70 0.02 106 31 34 3 838.07 6.64 107 31 35 4 725.31 6.49 108 31 36 5 120.29 0.15 109 31 37 6 120.28 0.14 110 32 35 3 826.49 0.18 111 32 37 5 340.62 110.20 112 32 199 167 134.32 7.16 113 32 200 168 131.81 5.92 114 32 203 171 157.09 0.62 115 32 240 208 121.95 0.81 116 33 199 166 156.59 0.56 117 33 203 170 159.00 0.46 118 33 228 195 159.30 0.33 119 33 233 200 160.03 0.34 120 35 199 164 160.08 0.34 121 35 233 198 160.22 0.41 122 35 237 202 160.25 0.41 123 35 238 203 160.25 0.41 124 35 239 204 160.23 0.41 125 35 240 205 160.05 0.33 126 36 238 202 160.31 0.43 127 36 239 203 160.25 0.41 128 36 240 204 160.23 0.41 129 37 200 163 160.25 0.40 130 37 240 203 160.24 0.41 131 37 241 204 160.24 0.41 132 37 242 205 159.84 0.42 133 38 67 29 160.35 0.39 134 38 68 30 160.34 0.39 135 38 69 31 160.33 0.39 136 38 70 32 160.38 0.41 137 38 192 154 160.40 0.42 138 38 239 201 160.38 0.41 139 38 240 202 160.25 0.41 140 38 241 203 160.25 0.41 141 38 242 204 160.16 0.39 142 39 42 3 897.47 1.14 143 39 68 29 160.38 0.41 144 39 242 203 160.25 0.41 145 40 242 202 160.25 0.41 146 40 244 204 160.06 0.33 147 40 245 205 159.43 0.24 148 41 68 27 628.09 3.83 149 42 47 5 665.71 22.66 150 42 67 25 694.27 2.80 151 42 68 26 677.57 5.55 152 43 46 3 871.36 1.53 153 44 47 3 863.34 1.30 154 44 48 4 855.79 0.88 155 44 95 51 558.62 5.71 156 45 48 3 854.81 1.37 157 45 95 50 627.55 4.11 158 46 67 21 703.29 3.82 159 47 66 19 703.34 3.80 160 47 67 20 703.29 3.82 161 47 68 21 703.25 3.83 162 47 91 44 687.01 0.23 163 48 65 17 703.44 3.77 164 48 66 18 703.33 3.81 165 48 67 19 703.32 3.81 166 48 73 25 701.75 3.68 167 48 91 43 687.09 0.24 168 48 92 44 686.95 0.20 169 48 95 47 686.91 0.21 170 48 96 48 686.58 0.06 171 49 64 15 703.61 3.73 172 49 65 16 703.45 3.77 173 49 67 18 703.41 3.77 174 50 62 12 703.84 3.71 175 50 63 13 703.77 3.71 176 50 64 14 703.62 3.73 177 50 96 46 687.08 0.21 178 50 97 47 687.03 0.20 179 51 62 11 703.86 3.71 180 51 63 12 703.77 3.71 181 51 65 14 703.76 3.71 182 51 72 21 703.01 3.74 183 51 154 103 512.43 1.31 184 51 158 107 445.19 1.51 185 52 60 8 704.17 3.75 186 52 61 9 704.13 3.75 187 52 62 10 703.86 3.71 188 53 60 7 704.25 3.71 189 53 61 8 704.15 3.75 190 53 63 10 704.13 3.74 191 53 132 79 543.74 0.94 192 53 136 83 543.53 0.92 193 53 154 101 512.51 1.34 194 53 158 105 477.91 1.25 195 54 58 4 704.84 3.70 196 54 59 5 704.53 3.62 197 54 60 6 704.26 3.71 198 54 136 82 543.62 0.93 199 55 58 3 812.19 3.69 200 55 59 4 727.56 15.10 201 55 133 78 543.69 0.91 202 55 136 81 543.63 0.93 203 55 137 82 543.63 0.94 204 56 129 73 543.88 0.96 205 56 133 77 543.76 0.94 206 59 136 77 543.62 0.93 207 59 140 81 543.14 0.96 208 60 140 80 543.15 0.96 209 61 75 14 701.83 3.69 210 61 76 15 701.70 3.73 211 61 77 16 701.36 3.68 212 61 136 75 543.52 0.92 213 61 139 78 543.41 0.94 214 61 140 79 543.14 0.96 215 61 154 93 512.66 1.36 216 62 75 13 701.81 3.69 217 62 76 14 701.60 3.67 218 62 97 35 687.20 0.23 219 63 72 9 703.06 3.77 220 63 74 11 702.13 3.52 221 63 75 12 701.48 3.62 222 63 97 34 687.16 0.24 223 63 139 76 543.41 0.94 224 63 151 88 536.37 0.03 225 63 154 91 512.47 1.32 226 64 72 8 703.05 3.77 227 64 73 9 702.15 3.52 228 64 74 10 701.81 3.68 229 64 96 32 687.14 0.24 230 64 97 33 687.08 0.21 231 65 70 5 703.33 3.77 232 65 71 6 703.01 3.79 233 65 72 7 702.13 3.52 234 66 69 3 850.57 3.23 235 66 70 4 704.26 3.73 236 66 71 5 702.82 3.80 237 66 73 7 701.79 3.68 238 68 241 173 162.78 0.66 239 68 245 177 159.37 0.19 240 69 163 94 438.95 1.35 241 69 192 123 302.03 2.04 242 69 241 172 163.69 0.21 243 70 159 89 444.88 1.56 244 70 162 92 438.98 1.36 245 70 163 93 438.94 1.36 246 70 192 122 304.75 3.40 247 70 239 169 181.06 4.19 248 71 163 92 438.97 1.36 249 72 84 12 687.82 0.18 250 72 154 82 508.96 0.07 251 72 155 83 499.72 2.96 252 72 158 86 445.19 1.52 253 73 83 10 687.84 0.18 254 73 84 11 687.81 0.18 255 73 88 15 687.50 0.21 256 73 89 16 687.23 0.21 257 73 92 19 687.27 0.23 258 74 82 8 687.88 0.20 259 74 83 9 687.84 0.18 260 75 81 6 687.95 0.18 261 75 82 7 687.91 0.19 262 75 97 22 687.24 0.23 263 75 139 64 543.40 0.94 264 75 142 67 543.08 0.95 265 75 151 76 536.43 0.01 266 76 79 3 901.78 6.42 267 76 80 4 799.92 0.98 268 76 81 5 687.96 0.18 269 76 97 21 687.24 0.23 270 76 100 24 686.99 0.22 271 77 80 3 861.75 5.64 272 77 82 5 812.64 3.52 273 77 140 63 543.14 0.96 274 79 100 21 687.02 0.22 275 79 101 22 687.00 0.21 276 80 100 20 686.98 0.21 277 80 101 21 686.86 0.19 278 80 103 23 683.56 0.23 279 81 97 16 687.29 0.25 280 81 100 19 686.97 0.21 281 81 101 20 686.73 0.12 282 81 102 21 673.50 0.68 283 81 103 22 657.16 7.44 284 82 103 21 627.79 12.84 285 82 105 23 602.71 7.84 286 82 142 60 542.99 0.90 287 83 89 6 687.72 0.14 288 83 92 9 687.54 0.23 289 83 93 10 687.44 0.26 290 83 96 13 687.39 0.24 291 83 97 14 687.33 0.24 292 83 102 19 664.03 4.00 293 83 103 20 627.78 12.84 294 83 104 21 588.66 0.82 295 83 105 22 588.57 0.86 296 84 89 5 721.41 4.79 297 84 105 21 567.93 9.31 298 84 107 23 566.60 9.40 299 84 108 24 547.25 0.06 300 84 109 25 546.93 0.10 301 84 114 30 547.02 0.06 302 84 151 67 538.07 0.03 303 85 104 19 602.85 7.78 304 85 105 20 588.60 0.86 305 85 107 22 566.95 9.31 306 85 108 23 547.91 0.08 307 86 89 3 851.57 3.85 308 86 90 4 831.13 3.56 309 86 108 22 547.44 0.15 310 86 109 23 547.44 0.15 311 87 90 3 833.14 3.83 312 87 91 4 831.14 3.56 313 88 91 3 838.98 7.24 314 88 92 4 833.46 4.14 315 89 92 3 841.28 0.23 316 89 93 4 841.28 0.23 317 90 93 3 845.00 1.32 318 90 94 4 844.24 1.71 319 91 94 3 847.84 0.84 320 91 95 4 845.26 2.22 321 92 95 3 847.55 3.87 322 92 96 4 831.66 3.27 323 93 102 9 652.45 9.79 324 94 98 4 846.11 0.83 325 95 98 3 880.27 0.34 326 97 100 3 853.94 3.66 327 105 142 37 543.12 0.96 328 105 151 46 540.90 0.46 329 106 142 36 543.33 0.94 330 107 113 6 743.83 4.67 331 107 114 7 743.81 4.68 332 107 117 10 546.77 0.10 333 107 147 40 542.62 0.99 334 107 148 41 542.43 1.06 335 108 113 5 765.09 13.09 336 108 114 6 773.13 9.07 337 109 114 5 795.04 1.88 338 110 113 3 844.62 0.06 339 110 114 4 828.85 2.94 340 111 114 3 835.77 0.53 341 111 115 4 828.85 2.93 342 111 122 11 545.11 0.54 343 111 152 41 542.03 1.03 344 111 155 44 539.98 0.95 345 111 156 45 539.56 1.14 346 111 160 49 503.73 1.76 347 111 189 78 335.69 4.30 348 112 115 3 853.94 2.68 349 112 116 4 830.06 2.33 350 113 116 3 840.40 2.85 351 113 117 4 821.29 0.85 352 114 117 3 830.79 3.54 353 114 118 4 818.36 2.31 354 114 148 34 542.57 1.01 355 114 151 37 542.12 1.02 356 114 152 38 541.57 1.20 357 114 155 41 540.00 0.93 358 115 118 3 834.90 5.59 359 115 119 4 804.77 4.23 360 115 120 5 757.63 21.24 361 115 121 6 545.21 0.58 362 115 122 7 622.72 39.33 363 115 152 37 542.13 1.00 364 116 119 3 820.80 0.58 365 117 148 31 542.61 0.99 366 118 148 30 542.61 0.99 367 118 149 31 542.57 1.01 368 118 152 34 542.19 0.96 369 120 124 4 818.45 2.30 370 120 125 5 784.25 2.88 371 121 124 3 854.88 2.45 372 121 125 4 839.98 1.04 373 122 125 3 843.25 2.68 374 122 152 30 542.39 0.89 375 122 156 34 540.96 0.44 376 122 181 59 424.25 0.18 377 122 186 64 373.21 1.65 378 122 189 67 357.84 2.62 379 122 190 68 355.63 3.64 380 123 180 57 425.37 0.20 381 123 186 63 375.93 2.02 382 124 180 56 425.38 0.20 383 125 152 27 541.65 0.67 384 125 153 28 541.23 0.49 385 125 180 55 424.61 0.18 386 125 181 56 424.56 0.15 387 126 131 5 816.13 1.08 388 126 153 27 541.15 0.52 389 126 178 52 426.48 0.06 390 126 179 53 426.36 0.04 391 126 180 54 424.44 0.26 392 127 131 4 816.16 1.09 393 127 153 26 539.92 0.84 394 127 156 29 537.83 0.14 395 127 171 44 433.75 0.59 396 127 174 47 433.25 0.46 397 127 178 51 426.47 0.06 398 127 179 52 426.36 0.04 399 127 181 54 424.25 0.24 400 128 131 3 837.26 2.43 401 128 132 4 816.28 1.11 402 128 174 46 433.00 0.54 403 128 178 50 426.47 0.06 404 129 132 3 832.62 4.75 405 129 133 4 820.22 2.83 406 129 171 42 433.75 0.58 407 129 174 45 433.00 0.54 408 129 175 46 431.78 0.42 409 130 133 3 834.01 4.05 410 130 134 4 830.65 1.15 411 130 178 48 426.48 0.06 412 131 134 3 837.51 2.25 413 131 135 4 830.65 1.15 414 131 153 22 538.36 0.40 415 131 178 47 426.47 0.06 416 132 135 3 847.13 1.61 417 132 136 4 830.11 1.46 418 132 153 21 538.14 0.29 419 132 154 22 537.62 0.04 420 132 157 25 512.61 1.42 421 132 158 26 512.65 1.39 422 133 136 3 839.40 3.19 423 133 137 4 837.64 2.29 424 134 137 3 845.18 0.28 425 134 138 4 837.66 2.28 426 135 138 3 843.46 0.58 427 135 139 4 837.71 2.31 428 135 150 15 538.41 0.38 429 135 153 18 537.52 0.01 430 135 154 19 536.88 0.32 431 136 139 3 853.95 4.61 432 136 140 4 837.69 2.31 433 136 154 18 533.13 0.40 434 137 140 3 837.71 2.31 435 137 141 4 834.35 3.98 436 138 141 3 856.80 0.54 437 138 142 4 814.24 0.45 438 138 143 5 767.46 2.77 439 138 146 8 542.60 1.00 440 138 150 12 540.84 0.38 441 139 142 3 834.05 4.13 442 139 147 8 541.52 0.60 443 139 150 11 539.96 0.79 444 139 151 12 539.95 0.79 445 139 154 15 528.53 1.90 446 142 147 5 674.50 18.25 447 143 146 3 854.10 3.17 448 143 147 4 817.69 0.27 449 144 147 3 842.16 2.80 450 146 149 3 846.28 0.72 451 146 150 4 814.21 0.79 452 147 150 3 822.90 2.35 453 147 151 4 812.97 0.17 454 148 151 3 829.94 2.66 455 148 152 4 829.94 2.66 456 149 152 3 829.94 2.66 457 149 153 4 829.95 2.67 458 150 153 3 837.22 6.30 459 150 154 4 823.33 0.65 460 151 154 3 837.42 6.41 461 151 155 4 812.99 0.17 462 152 156 4 812.00 0.03 463 153 156 3 840.90 1.95 464 153 157 4 818.10 3.08 465 154 157 3 830.63 3.19 466 154 158 4 818.10 3.08 467 155 158 3 835.05 0.98 468 155 159 4 806.88 2.50 469 155 160 5 794.22 0.58 470 156 160 4 763.12 1.76 471 156 161 5 799.80 5.87 472 156 167 11 433.98 0.63 473 156 181 25 405.43 2.84 474 156 186 30 335.94 4.45 475 156 189 33 335.38 4.33 476 157 161 4 820.65 1.72 477 157 171 14 433.74 0.60 478 158 161 3 843.62 0.55 479 158 162 4 823.21 0.51 480 159 162 3 845.32 1.33 481 159 163 4 824.75 1.07 482 160 163 3 831.61 2.36 483 160 164 4 819.32 3.79 484 160 167 7 662.19 42.80 485 160 189 29 327.50 0.40 486 161 164 3 834.78 3.95 487 161 167 6 521.70 44.49 488 161 168 7 433.97 0.61 489 161 171 10 433.93 0.64 490 162 238 76 183.61 4.60 491 162 239 77 183.50 4.65 492 163 192 29 309.97 5.92 493 163 237 74 183.90 4.49 494 163 238 75 183.60 4.61 495 163 239 76 182.81 4.30 496 164 167 3 834.64 0.67 497 164 168 4 819.60 1.10 498 164 188 24 335.88 4.41 499 164 189 25 335.88 4.42 500 164 192 28 326.50 0.70 501 164 195 31 324.19 1.92 502 164 236 72 184.57 4.82 503 164 237 73 184.79 4.93 504 165 168 3 831.67 2.16 505 165 169 4 825.37 3.98 506 165 236 71 184.81 4.92 507 165 238 73 185.19 5.00 508 166 169 3 825.39 3.99 509 166 170 4 834.73 0.68 510 166 182 16 411.66 5.96 511 166 184 18 375.81 2.15 512 166 185 19 358.38 5.30 513 166 188 22 336.64 4.75 514 166 236 70 185.83 4.74 515 167 170 3 835.46 1.04 516 167 171 4 838.00 2.31 517 167 185 18 357.77 5.02 518 167 188 21 336.61 4.76 519 167 189 22 336.00 4.47 520 168 171 3 853.80 2.65 521 168 172 4 839.12 1.75 522 169 172 3 839.13 1.75 523 169 173 4 838.65 0.01 524 170 173 3 848.90 5.13 525 170 174 4 837.20 0.76 526 170 181 11 421.48 1.05 527 170 182 12 421.48 1.05 528 170 185 15 375.56 1.96 529 171 174 3 841.10 1.19 530 171 175 4 837.70 0.51 531 172 175 3 850.60 5.95 532 172 176 4 869.60 2.27 533 173 176 3 907.81 0.54 534 174 177 3 846.44 4.79 535 174 178 4 787.64 10.04 536 174 179 5 761.52 1.60 537 174 180 6 767.38 4.00 538 174 181 7 544.54 46.76 539 174 182 8 524.88 36.94 540 181 185 4 824.87 1.73 541 181 186 5 817.37 1.79 542 182 185 3 835.34 0.46 543 182 186 4 824.88 1.74 544 183 186 3 836.90 1.59 545 183 187 4 824.86 1.73 546 183 216 33 282.96 2.86 547 184 187 3 831.04 1.35 548 184 188 4 840.92 3.59 549 184 216 32 282.97 2.87 550 184 217 33 282.98 2.87 551 184 218 34 283.04 2.88 552 185 188 3 856.66 0.99 553 185 189 4 839.82 4.15 554 186 189 3 840.93 3.60 555 186 190 4 839.82 4.15 556 187 190 3 845.36 1.38 557 187 191 4 839.29 4.41 558 187 215 28 284.57 2.08 559 187 216 29 284.88 2.15 560 188 191 3 839.59 4.25 561 188 192 4 805.45 1.19 562 188 195 7 756.00 7.04 563 188 215 27 285.80 2.08 564 188 232 44 187.59 4.40 565 188 236 48 186.12 4.81 566 189 192 3 845.96 4.39 567 191 194 3 830.69 1.44 568 191 195 4 817.98 4.96 569 191 213 22 290.16 0.73 570 191 214 23 290.17 0.74 571 191 215 24 289.87 0.88 572 192 195 3 835.69 1.05 573 192 196 4 830.75 1.42 574 192 237 45 186.12 4.80 575 192 239 47 185.99 4.77 576 192 240 48 183.52 3.98 577 192 241 49 183.25 4.08 578 193 196 3 836.48 0.65 579 193 197 4 830.61 1.34 580 193 241 48 179.47 2.19 581 193 246 53 174.63 2.02 582 194 197 3 836.61 0.58 583 194 198 4 836.48 0.65 584 194 213 19 291.39 1.23 585 195 198 3 839.34 1.78 586 195 199 4 831.61 0.83 587 195 209 14 291.87 1.20 588 195 213 18 291.40 1.26 589 195 225 30 291.34 1.28 590 195 232 37 195.58 3.12 591 195 237 42 186.57 4.61 592 196 199 3 839.40 1.75 593 196 200 4 837.16 0.63 594 196 237 41 186.68 4.66 595 196 240 44 184.65 4.42 596 196 241 45 183.35 4.08 597 196 246 50 176.22 2.44 598 197 200 3 839.40 1.75 599 197 201 4 831.30 2.31 600 197 246 49 176.69 2.20 601 197 247 50 175.72 2.19 602 198 201 3 837.12 0.59 603 198 202 4 831.31 2.31 604 198 209 11 292.10 1.32 605 198 210 12 292.39 1.47 606 198 211 13 292.28 1.42 607 198 213 15 292.01 1.29 608 199 202 3 836.25 0.15 609 199 203 4 833.62 1.16 610 199 209 10 292.42 1.47 611 199 233 34 207.24 2.69 612 199 237 38 187.75 4.15 613 199 240 41 186.56 4.59 614 200 203 3 850.14 3.02 615 200 240 40 186.56 4.58 616 200 241 41 186.56 4.58 617 200 242 42 186.44 4.56 618 200 243 43 184.65 4.30 619 201 204 3 856.89 0.35 620 201 205 4 833.49 1.11 621 201 210 9 292.46 1.44 622 202 205 3 829.81 0.72 623 202 206 4 814.16 4.07 624 202 207 5 307.66 5.37 625 202 208 6 292.91 1.23 626 202 209 7 292.50 1.44 627 202 228 26 292.02 1.65 628 203 228 25 292.05 1.66 629 205 208 3 832.50 2.35 630 205 209 4 831.01 2.94 631 205 210 5 746.60 3.77 632 207 227 20 292.45 1.65 633 207 228 21 292.29 1.65 634 208 226 18 292.53 1.64 635 208 227 19 292.30 1.73 636 209 212 3 855.43 0.30 637 209 213 4 843.21 2.96 638 209 225 16 292.87 1.50 639 209 226 17 292.38 1.71 640 209 227 18 292.37 1.71 641 209 228 19 292.37 1.71 642 209 233 24 221.92 1.79 643 212 224 12 293.13 1.57 644 212 225 13 293.01 1.54 645 212 226 14 292.79 1.52 646 213 224 11 293.19 1.58 647 213 225 12 293.02 1.54 648 214 222 8 293.36 1.57 649 214 223 9 293.31 1.55 650 214 224 10 293.28 1.55 651 215 222 7 293.39 1.55 652 215 223 8 293.31 1.55 653 215 225 10 293.30 1.54 654 215 232 17 254.42 20.78 655 216 221 5 293.42 1.56 656 216 222 6 293.39 1.55 657 217 220 3 832.90 0.43 658 217 221 4 295.10 2.39 659 217 223 6 293.38 1.54 660 225 230 5 302.64 1.66 661 225 232 7 298.00 0.10 662 227 230 3 843.35 5.60 663 227 233 6 652.28 29.88 664 228 233 5 652.26 29.88 665 228 234 6 582.88 4.79 666 229 234 5 673.70 19.16 667 230 233 3 833.11 0.21 668 230 234 4 793.58 10.72 669 231 234 3 833.10 0.21 670 231 235 4 708.01 36.30 671 231 236 5 674.89 19.75 672 232 236 4 707.75 36.20 673 232 237 5 582.89 4.80 674 233 237 4 594.19 10.46 675 237 240 3 941.09 1.53 676 241 245 4 832.61 2.03 677 241 246 5 803.99 4.62 678 242 245 3 853.93 0.43 679 242 246 4 832.62 2.03 680 243 246 3 832.61 2.02 681 243 247 4 811.67 8.44