BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 17 15 35.13 2.14 2 2 20 18 24.16 0.07 3 2 21 19 23.98 0.12 4 2 24 22 23.98 0.12 5 2 49 47 23.97 0.12 6 2 50 48 24.11 0.05 7 2 51 49 35.61 2.14 8 3 51 48 36.24 2.21 9 4 9 5 721.99 30.37 10 4 13 9 43.20 0.96 11 4 14 10 36.53 2.21 12 4 17 13 36.38 2.28 13 4 51 47 36.57 2.33 14 4 52 48 36.46 2.31 15 4 53 49 57.09 0.14 16 4 129 125 59.33 0.04 17 4 132 128 59.11 0.09 18 5 9 4 776.88 9.64 19 5 53 48 57.28 0.24 20 6 9 3 817.88 3.58 21 6 53 47 59.19 0.05 22 6 54 48 59.32 0.04 23 6 55 49 59.43 0.02 24 6 129 123 59.36 0.05 25 6 132 126 59.35 0.06 26 6 133 127 59.35 0.06 27 6 136 130 59.36 0.06 28 7 55 48 59.69 0.07 29 9 13 4 828.24 1.97 30 9 129 120 78.83 0.63 31 9 175 166 85.48 0.06 32 10 13 3 847.21 1.70 33 10 14 4 830.69 0.74 34 10 175 165 90.23 0.18 35 11 14 3 847.89 1.36 36 11 15 4 841.40 4.61 37 11 172 161 79.50 0.29 38 11 173 162 86.63 0.22 39 11 175 164 92.92 0.10 40 12 15 3 847.22 1.71 41 12 16 4 845.35 2.64 42 13 16 3 854.03 1.70 43 13 17 4 836.67 6.98 44 14 17 3 835.29 1.52 45 14 18 4 835.29 1.52 46 14 129 115 98.25 1.33 47 14 157 143 93.34 0.04 48 14 161 147 93.08 0.15 49 14 168 154 93.12 0.17 50 14 171 157 95.94 0.38 51 14 172 158 94.98 0.85 52 15 18 3 846.13 2.14 53 15 19 4 830.76 0.74 54 15 168 153 93.56 0.15 55 16 19 3 846.13 2.14 56 16 20 4 828.43 1.91 57 17 20 3 852.74 6.48 58 17 21 4 829.96 1.15 59 17 50 33 105.88 0.31 60 17 52 35 117.34 0.60 61 17 158 141 99.73 1.15 62 18 21 3 841.64 5.76 63 18 22 4 831.11 0.58 64 18 157 139 104.78 0.79 65 18 158 140 99.73 1.15 66 18 161 143 99.72 1.14 67 18 162 144 98.42 0.67 68 18 168 150 99.73 1.15 69 19 22 3 841.50 0.38 70 19 23 4 826.13 1.92 71 20 23 3 845.02 7.53 72 20 24 4 826.12 1.92 73 21 24 3 844.01 3.61 74 21 25 4 826.70 1.62 75 21 50 29 119.45 0.48 76 21 64 43 118.65 0.30 77 21 158 137 118.45 0.40 78 21 162 141 112.25 0.01 79 22 25 3 848.44 1.01 80 22 161 139 118.73 0.30 81 22 162 140 115.89 0.07 82 22 164 142 118.45 0.40 83 22 165 143 118.95 0.42 84 22 168 146 119.11 0.37 85 22 238 216 108.90 1.66 86 22 239 217 105.90 0.32 87 25 37 12 120.24 0.09 88 25 64 39 120.55 0.16 89 25 66 41 120.41 0.18 90 25 69 44 120.39 0.17 91 25 239 214 120.33 0.05 92 26 37 11 120.44 0.14 93 26 38 12 120.53 0.14 94 26 41 15 120.60 0.13 95 26 66 40 120.55 0.16 96 27 30 3 936.55 0.85 97 27 35 8 120.55 0.14 98 27 36 9 120.48 0.15 99 27 37 10 120.45 0.14 100 27 38 11 120.53 0.14 101 28 36 8 121.14 0.38 102 28 38 10 120.68 0.15 103 29 35 6 122.94 1.13 104 29 36 7 121.87 0.74 105 30 33 3 846.12 3.42 106 30 34 4 492.66 96.25 107 30 35 5 122.95 1.14 108 30 36 6 122.76 1.10 109 31 34 3 830.77 0.44 110 31 36 5 198.44 38.86 111 31 199 168 140.22 9.79 112 31 200 169 132.32 5.90 113 31 203 172 141.56 10.46 114 31 240 209 123.17 1.22 115 32 199 167 141.59 10.45 116 32 202 170 141.62 10.46 117 32 203 171 141.62 10.46 118 32 207 175 141.71 10.52 119 32 228 196 141.59 10.46 120 34 233 199 155.38 3.76 121 34 237 203 155.37 3.72 122 34 238 204 155.31 3.69 123 34 239 205 155.30 3.70 124 34 240 206 155.28 3.70 125 35 238 203 155.46 3.68 126 35 239 204 155.31 3.69 127 35 240 205 155.30 3.70 128 36 200 164 155.37 3.72 129 36 240 204 155.31 3.70 130 36 241 205 155.31 3.70 131 36 242 206 155.04 3.58 132 37 66 29 155.50 3.65 133 37 67 30 155.49 3.66 134 37 68 31 155.48 3.66 135 37 69 32 155.50 3.65 136 37 192 155 155.56 3.69 137 37 239 202 155.54 3.69 138 37 240 203 155.43 3.68 139 37 241 204 155.33 3.70 140 37 242 205 155.29 3.70 141 38 41 3 919.39 4.57 142 38 67 29 155.50 3.65 143 38 242 204 155.34 3.71 144 39 242 203 155.34 3.71 145 39 244 205 155.29 3.70 146 39 245 206 154.58 3.43 147 40 46 6 743.84 26.40 148 41 45 4 819.21 2.86 149 41 46 5 743.84 26.40 150 41 66 25 672.39 25.27 151 41 67 26 672.38 25.27 152 42 45 3 845.11 0.93 153 42 46 4 819.21 2.85 154 43 46 3 836.72 5.13 155 43 47 4 836.72 5.13 156 43 94 51 575.00 40.68 157 44 94 50 586.65 34.86 158 45 66 21 727.57 19.20 159 46 65 19 727.59 19.21 160 46 66 20 727.57 19.20 161 46 67 21 727.56 19.20 162 47 64 17 727.61 19.21 163 47 65 18 727.60 19.21 164 47 66 19 727.58 19.20 165 47 72 25 727.19 19.23 166 47 90 43 611.65 35.19 167 47 91 44 611.35 35.04 168 47 94 47 611.30 34.90 169 47 95 48 611.01 34.84 170 48 63 15 727.71 19.19 171 48 64 16 727.63 19.21 172 48 66 18 727.61 19.21 173 49 62 13 727.77 19.20 174 49 63 14 727.72 19.19 175 49 95 46 649.85 16.02 176 49 96 47 676.39 2.64 177 50 61 11 727.81 19.19 178 50 62 12 727.78 19.20 179 50 64 14 727.75 19.20 180 50 158 108 448.67 1.94 181 51 59 8 728.30 19.28 182 51 60 9 728.12 19.21 183 51 61 10 727.82 19.20 184 52 59 7 728.31 19.28 185 52 60 8 728.29 19.28 186 52 62 10 728.12 19.21 187 52 132 80 538.94 1.26 188 52 136 84 540.25 0.49 189 52 154 102 501.26 1.23 190 52 158 106 467.69 0.41 191 53 57 4 728.50 19.25 192 53 58 5 728.40 19.28 193 53 59 6 728.31 19.29 194 53 136 83 541.25 0.12 195 54 57 3 809.21 1.14 196 54 58 4 728.50 19.32 197 54 133 79 539.86 0.81 198 54 136 82 541.27 0.12 199 54 137 83 541.45 0.03 200 55 130 75 541.25 0.17 201 55 133 78 541.24 0.15 202 58 136 78 541.50 0.01 203 58 137 79 541.49 0.01 204 58 140 82 541.43 0.06 205 58 141 83 541.48 0.09 206 59 140 81 541.39 0.07 207 60 74 14 727.04 19.14 208 60 75 15 727.00 19.15 209 60 76 16 726.87 19.14 210 60 136 76 540.98 0.01 211 60 139 79 541.38 0.04 212 60 140 80 541.24 0.13 213 61 74 13 727.02 19.14 214 61 75 14 726.92 19.12 215 61 96 35 684.08 1.00 216 62 71 9 727.40 19.21 217 62 73 11 727.22 19.23 218 62 74 12 726.90 19.12 219 62 96 34 683.99 0.97 220 62 154 92 499.22 0.55 221 63 71 8 727.41 19.21 222 63 72 9 727.30 19.25 223 63 73 10 727.20 19.23 224 63 95 32 676.51 2.69 225 63 96 33 681.37 0.16 226 64 69 5 727.52 19.18 227 64 70 6 727.43 19.22 228 64 71 7 727.33 19.25 229 64 72 8 727.27 19.23 230 65 68 3 859.53 1.30 231 65 69 4 727.44 19.22 232 65 70 5 727.40 19.22 233 65 72 7 727.25 19.25 234 67 241 174 172.16 5.28 235 67 245 178 153.32 4.79 236 68 163 95 444.49 0.69 237 68 192 124 273.13 1.19 238 68 241 173 163.75 0.55 239 69 159 90 445.16 0.94 240 69 162 93 444.53 0.70 241 69 163 94 444.49 0.69 242 69 192 123 274.66 1.95 243 69 239 170 195.72 1.23 244 70 163 93 444.53 0.70 245 71 83 12 611.90 35.31 246 71 154 83 496.59 0.75 247 71 155 84 483.37 0.66 248 71 158 87 448.74 1.98 249 71 159 88 448.66 1.95 250 72 82 10 611.90 35.31 251 72 83 11 611.89 35.31 252 72 87 15 612.07 35.22 253 72 88 16 611.80 35.20 254 72 91 19 611.99 35.11 255 73 81 8 612.04 35.25 256 73 82 9 611.90 35.30 257 73 96 23 733.62 25.78 258 74 80 6 632.25 25.43 259 74 81 7 612.38 35.30 260 74 96 22 733.64 25.78 261 74 139 65 541.17 0.15 262 74 142 68 541.17 0.29 263 74 151 77 537.73 0.64 264 75 78 3 909.52 0.64 265 75 79 4 814.48 10.54 266 75 80 5 639.70 21.70 267 75 96 21 733.64 25.78 268 75 98 23 739.88 28.91 269 75 99 24 739.34 29.09 270 76 79 3 855.12 4.78 271 76 81 5 796.66 0.90 272 76 140 64 541.23 0.15 273 78 99 21 739.45 29.11 274 78 100 22 739.40 29.12 275 78 101 23 739.37 29.11 276 79 101 22 739.25 29.16 277 79 103 24 733.52 32.02 278 80 96 16 739.97 28.89 279 80 99 19 739.46 29.15 280 80 101 21 739.30 29.20 281 80 102 22 727.11 35.26 282 80 103 23 723.77 36.92 283 81 101 20 739.34 29.21 284 81 103 22 701.48 48.07 285 81 105 24 682.75 57.41 286 81 142 61 541.15 0.34 287 82 88 6 740.43 28.86 288 82 92 10 740.39 28.86 289 82 95 13 740.38 28.85 290 82 96 14 740.32 28.82 291 82 102 20 727.19 35.29 292 82 103 21 723.79 36.96 293 82 104 22 682.77 57.43 294 82 105 23 682.75 57.42 295 83 88 5 740.71 28.72 296 83 105 22 682.77 57.44 297 83 107 24 671.37 63.02 298 83 108 25 659.84 57.27 299 83 109 26 649.87 52.29 300 83 114 31 626.13 40.41 301 83 151 68 539.82 0.98 302 83 155 72 501.30 1.23 303 84 104 20 682.85 57.43 304 84 105 21 682.79 57.44 305 84 107 23 671.39 63.04 306 84 108 24 671.25 62.96 307 85 88 3 842.89 0.24 308 85 89 4 825.52 1.62 309 85 108 23 671.28 62.98 310 85 109 24 671.28 62.98 311 86 89 3 840.77 0.82 312 86 90 4 833.98 4.21 313 87 90 3 843.37 0.48 314 87 91 4 836.37 3.01 315 88 91 3 840.33 1.04 316 88 92 4 850.89 4.22 317 89 92 3 852.17 3.59 318 89 93 4 853.75 2.80 319 90 93 3 855.72 1.81 320 90 94 4 853.75 2.79 321 91 94 3 853.75 2.79 322 91 95 4 836.39 3.02 323 92 95 3 852.21 2.02 324 92 102 10 723.85 36.98 325 93 97 4 843.63 5.46 326 94 97 3 883.88 1.26 327 96 99 3 856.56 3.97 328 105 142 37 541.51 0.49 329 105 151 46 540.61 0.73 330 106 113 7 768.70 18.74 331 107 113 6 768.69 18.74 332 107 114 7 750.57 9.68 333 107 117 10 623.84 39.59 334 107 147 40 541.31 0.72 335 107 148 41 541.27 0.72 336 107 151 44 541.19 0.78 337 108 113 5 812.70 3.26 338 108 114 6 812.70 3.25 339 109 114 5 812.77 3.25 340 110 113 3 855.28 3.33 341 110 114 4 824.47 2.58 342 111 114 3 833.92 7.31 343 111 115 4 824.48 2.58 344 111 122 11 543.42 0.70 345 111 152 41 541.20 0.78 346 111 155 44 540.36 0.60 347 111 156 45 540.21 0.53 348 111 160 49 498.54 0.59 349 111 189 78 303.46 4.24 350 112 115 3 846.39 1.07 351 112 116 4 824.62 2.50 352 113 116 3 840.03 1.23 353 113 117 4 824.62 2.50 354 114 117 3 827.44 0.43 355 114 118 4 821.27 0.82 356 114 148 34 541.44 0.80 357 114 152 38 541.23 0.83 358 114 155 41 540.39 0.62 359 115 118 3 826.76 1.92 360 115 119 4 812.80 3.42 361 115 120 5 746.11 26.00 362 115 121 6 545.40 0.28 363 115 122 7 545.40 0.27 364 115 152 37 541.37 0.82 365 116 119 3 824.10 1.60 366 117 148 31 541.50 0.83 367 118 148 30 541.49 0.83 368 118 149 31 541.48 0.84 369 118 152 34 541.43 0.86 370 120 124 4 822.56 1.56 371 120 125 5 818.50 1.22 372 120 126 6 820.85 2.00 373 121 124 3 851.74 3.93 374 121 125 4 841.75 8.92 375 122 125 3 851.73 3.93 376 122 152 30 541.47 0.84 377 122 156 34 540.86 0.84 378 122 181 59 428.78 1.29 379 122 186 64 379.39 5.09 380 122 189 67 360.34 1.58 381 122 190 68 359.38 1.16 382 123 180 57 429.80 0.82 383 123 181 58 428.79 1.29 384 123 183 60 427.64 1.01 385 123 186 63 392.06 1.20 386 124 180 56 430.11 0.98 387 125 149 24 541.55 0.84 388 125 152 27 541.28 0.96 389 125 153 28 541.23 0.97 390 125 180 55 429.82 0.83 391 125 181 56 429.78 0.82 392 126 131 5 810.67 0.32 393 126 153 27 541.23 1.01 394 126 178 52 430.63 0.83 395 126 179 53 430.51 0.80 396 126 180 54 429.80 0.82 397 127 131 4 817.60 0.43 398 127 153 26 541.17 1.03 399 127 156 29 540.84 1.19 400 127 171 44 442.33 1.04 401 127 178 51 430.63 0.83 402 127 179 52 430.52 0.79 403 127 181 54 428.48 1.16 404 128 131 3 847.34 7.40 405 128 132 4 837.43 2.45 406 128 171 43 442.25 1.03 407 128 174 46 434.00 0.04 408 128 178 50 430.62 0.83 409 128 179 51 430.62 0.83 410 129 132 3 847.35 7.40 411 129 133 4 835.36 3.48 412 129 171 42 442.25 1.03 413 129 174 45 435.12 0.60 414 129 175 46 433.54 0.11 415 130 133 3 835.39 3.50 416 130 134 4 827.59 0.40 417 130 174 44 435.76 0.84 418 130 175 45 433.61 0.09 419 130 176 46 433.35 0.14 420 130 177 47 430.73 0.82 421 130 178 48 430.65 0.83 422 131 134 3 853.11 2.42 423 131 135 4 822.90 2.74 424 131 153 22 541.17 1.09 425 131 178 47 430.63 0.83 426 132 135 3 838.33 2.79 427 132 136 4 817.90 0.21 428 132 153 21 540.91 1.23 429 132 154 22 540.90 1.23 430 132 157 25 521.03 11.11 431 133 136 3 841.60 1.60 432 133 137 4 829.29 0.88 433 134 137 3 838.75 3.85 434 134 138 4 826.08 2.47 435 135 138 3 838.75 3.85 436 135 139 4 823.88 3.57 437 135 150 15 541.25 1.08 438 135 153 18 540.92 1.24 439 136 139 3 845.50 0.48 440 136 140 4 816.93 0.12 441 137 140 3 824.06 0.01 442 137 141 4 816.91 0.12 443 138 141 3 841.23 0.23 444 138 142 4 820.58 1.73 445 138 143 5 805.68 1.28 446 138 146 8 541.86 0.99 447 138 147 9 541.82 0.99 448 138 150 12 541.39 1.15 449 139 142 3 818.84 2.60 450 139 147 8 541.79 1.02 451 139 150 11 541.47 1.19 452 139 151 12 541.41 1.16 453 139 154 15 528.92 7.39 454 142 147 5 678.85 15.50 455 143 146 3 861.40 0.74 456 143 147 4 806.99 1.16 457 144 147 3 834.78 0.23 458 146 149 3 869.08 5.17 459 146 150 4 820.64 0.41 460 147 150 3 844.00 0.73 461 147 151 4 820.71 0.38 462 148 151 3 832.81 2.58 463 148 152 4 822.24 0.40 464 149 152 3 835.11 3.72 465 149 153 4 825.96 0.85 466 150 153 3 842.10 3.88 467 150 154 4 820.75 0.29 468 151 154 3 820.70 0.26 469 151 155 4 823.98 1.91 470 152 155 3 835.17 0.12 471 152 156 4 827.30 0.26 472 153 156 3 835.22 0.10 473 153 157 4 823.29 2.26 474 154 157 3 837.76 4.97 475 154 158 4 830.18 4.83 476 155 158 3 852.01 2.16 477 155 159 4 783.75 18.36 478 155 160 5 795.11 7.12 479 156 160 4 794.25 7.55 480 156 161 5 795.63 6.87 481 156 167 11 496.44 28.00 482 156 171 15 442.32 1.05 483 156 189 33 287.24 0.06 484 157 161 4 811.60 3.25 485 157 171 14 442.23 1.05 486 158 161 3 839.80 1.06 487 158 162 4 830.31 0.83 488 159 162 3 835.97 0.86 489 159 163 4 830.31 0.83 490 160 163 3 832.88 2.11 491 160 164 4 825.07 3.45 492 160 167 7 564.34 7.91 493 160 189 29 287.22 0.04 494 161 164 3 843.93 1.79 495 161 167 6 550.49 0.99 496 161 168 7 442.72 1.25 497 161 171 10 442.32 1.05 498 162 238 76 196.88 1.66 499 162 239 77 196.68 1.58 500 163 192 29 274.75 1.92 501 163 237 74 197.64 1.78 502 163 238 75 196.89 1.66 503 163 239 76 195.72 1.23 504 164 167 3 839.73 0.06 505 164 168 4 825.87 5.55 506 164 188 24 294.75 0.10 507 164 192 28 285.75 0.17 508 164 195 31 283.18 1.09 509 164 232 68 198.66 1.83 510 164 236 72 198.02 1.71 511 164 237 73 197.64 1.78 512 165 168 3 827.24 4.87 513 165 169 4 825.19 4.83 514 165 236 71 198.03 1.70 515 165 238 73 197.81 1.71 516 166 169 3 835.46 0.95 517 166 170 4 829.25 1.17 518 166 184 18 366.40 2.25 519 166 185 19 357.02 2.44 520 166 188 22 340.64 10.53 521 166 236 70 198.05 1.69 522 167 170 3 846.78 4.67 523 167 171 4 826.43 5.46 524 167 185 18 347.94 6.88 525 167 188 21 336.81 12.26 526 167 189 22 336.81 12.26 527 168 171 3 849.24 2.27 528 168 172 4 834.62 1.38 529 169 172 3 845.88 2.75 530 169 173 4 834.81 1.37 531 170 173 3 860.06 0.48 532 170 174 4 843.57 3.91 533 170 179 9 430.50 0.82 534 170 185 15 369.50 0.78 535 171 174 3 865.03 2.98 536 171 179 8 430.53 0.80 537 172 175 3 878.58 2.52 538 173 177 4 881.77 0.93 539 174 177 3 850.33 3.41 540 174 178 4 815.92 4.39 541 174 179 5 799.47 12.62 542 179 185 6 834.59 1.07 543 181 185 4 834.58 1.07 544 181 186 5 827.07 4.83 545 182 185 3 867.48 5.09 546 182 186 4 834.60 1.08 547 183 186 3 834.62 1.08 548 183 187 4 834.60 1.08 549 183 216 33 250.61 0.97 550 184 187 3 841.94 3.10 551 184 188 4 835.05 1.31 552 184 216 32 250.61 0.97 553 184 217 33 250.77 0.91 554 184 218 34 250.77 0.92 555 185 188 3 841.08 2.03 556 185 189 4 837.35 0.17 557 186 189 3 837.78 0.39 558 186 190 4 817.01 5.58 559 187 190 3 837.35 0.17 560 187 191 4 804.38 0.72 561 187 215 28 251.25 1.05 562 187 216 29 251.25 1.06 563 188 191 3 843.15 2.46 564 188 192 4 816.90 5.67 565 188 215 27 251.36 1.00 566 188 232 44 198.84 1.84 567 188 236 48 198.16 1.71 568 189 192 3 860.50 6.19 569 191 194 3 842.01 1.61 570 191 195 4 817.29 5.48 571 191 213 22 252.75 0.48 572 191 215 24 252.76 0.49 573 192 195 3 831.72 1.47 574 192 196 4 831.45 1.59 575 192 237 45 197.72 1.74 576 192 239 47 197.56 1.81 577 192 240 48 196.59 1.63 578 192 241 49 195.24 1.33 579 193 196 3 833.54 0.56 580 193 197 4 831.45 1.60 581 193 241 48 195.12 1.27 582 193 246 53 170.37 4.36 583 194 197 3 836.32 0.07 584 194 198 4 823.99 5.33 585 194 213 19 254.86 0.56 586 195 198 3 849.99 6.50 587 195 199 4 831.30 1.04 588 195 209 14 254.97 0.52 589 195 213 18 254.89 0.56 590 195 215 20 254.88 0.57 591 195 232 37 215.75 9.85 592 195 237 42 198.03 1.66 593 196 199 3 843.97 3.93 594 196 200 4 832.83 1.80 595 196 240 44 196.69 1.60 596 196 241 45 196.60 1.64 597 196 246 50 174.75 2.54 598 197 200 3 836.33 0.07 599 197 201 4 833.88 1.28 600 197 246 49 176.57 3.32 601 198 201 3 841.36 5.01 602 198 202 4 832.74 0.70 603 198 209 11 255.11 0.55 604 198 210 12 255.30 0.46 605 198 211 13 255.31 0.46 606 198 213 15 255.23 0.47 607 199 202 3 835.80 0.06 608 199 203 4 835.01 0.44 609 199 209 10 255.31 0.46 610 199 233 34 224.59 5.48 611 199 237 38 199.11 2.15 612 199 240 41 197.65 1.79 613 200 203 3 847.43 4.38 614 200 204 4 837.28 0.69 615 200 241 41 197.62 1.79 616 200 242 42 197.24 1.81 617 200 243 43 196.16 1.33 618 200 246 46 195.35 1.33 619 201 204 3 837.28 0.69 620 201 205 4 832.65 0.64 621 201 210 9 258.63 1.16 622 202 205 3 832.60 0.61 623 202 206 4 831.93 0.95 624 202 207 5 289.24 6.67 625 202 208 6 260.89 0.03 626 202 209 7 258.63 1.16 627 202 228 26 255.30 0.47 628 205 208 3 826.14 3.23 629 207 227 20 256.61 0.90 630 207 228 21 255.55 0.56 631 208 226 18 256.91 0.88 632 208 227 19 256.83 0.85 633 209 212 3 880.40 0.18 634 209 213 4 858.20 1.07 635 209 225 16 257.34 0.83 636 209 226 17 256.93 0.87 637 209 227 18 256.91 0.88 638 209 228 19 256.91 0.88 639 209 233 24 256.91 0.88 640 212 224 12 257.53 0.87 641 212 225 13 257.38 0.83 642 212 226 14 257.30 0.83 643 213 224 11 257.86 0.73 644 213 225 12 257.44 0.85 645 214 222 8 260.04 0.13 646 214 223 9 259.53 0.09 647 214 224 10 257.86 0.73 648 215 222 7 260.13 0.18 649 215 223 8 259.63 0.12 650 215 225 10 259.47 0.05 651 215 232 17 257.26 0.81 652 216 220 4 300.37 2.25 653 216 221 5 281.12 10.52 654 216 222 6 260.20 0.14 655 217 220 3 827.71 6.16 656 217 221 4 300.37 2.25 657 217 223 6 260.99 0.47 658 225 230 5 312.78 1.57 659 225 232 7 312.83 1.57 660 227 230 3 854.47 1.08 661 227 233 6 324.40 3.67 662 228 233 5 314.60 1.24 663 228 234 6 314.62 1.25 664 229 234 5 482.80 75.54 665 230 233 3 839.37 2.60 666 230 234 4 814.02 5.51 667 231 234 3 830.84 2.90 668 231 235 4 650.29 27.89 669 231 236 5 482.83 75.56 670 232 236 4 473.04 80.45 671 232 237 5 473.00 80.45 672 233 237 4 472.93 80.50 673 237 240 3 936.32 0.18 674 241 245 4 832.32 0.30 675 241 246 5 704.68 54.01 676 242 245 3 832.34 0.31 677 242 246 4 832.33 0.30 678 243 246 3 817.97 6.90